Spo00222 (gene) | SpinachBase

Overview


Name	Spo00222
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Unknown protein
Location	chr1 : 31162606 .. 31163004 (-)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTCCAACTTCCTTCACAAAGTTGAACCCGTCGATTCTTTCTGGAACTGGAAACAGCATCAATCCCGCAAAAACAACAATATCAACAAATGGAAGTTTGAAGACAAACGGTTCTCTGCGCCGGAACTTTTCGGCACCAATCGCCGGAAAAACGGCGCAACTGAGGCGTTCATTTTGGGCCCGGGAGCTGGGGCTGGGGTTGGTTGTGGTGTTGGGTTCGGGTTTGGGCTGGTAGGCGGAGCTGGGTTTTCTGGTGGGCTTTGGAATCACATGCATCTTGCTTTCGGGTTTGGGTACGGTTGCGGTGCGGGAGCTGGACTTGGATTTGGGCAGGGGTTGGGTTATGGTTCAAGTTGGAAAACGATTAAGTCGGACGTGCTTGGATTTGAGGAGTAA

mRNA sequence

ATGGATTCCAACTTCCTTCACAAAGTTGAACCCGTCGATTCTTTCTGGAACTGGAAACAGCATCAATCCCGCAAAAACAACAATATCAACAAATGGAAGTTTGAAGACAAACGGTTCTCTGCGCCGGAACTTTTCGGCACCAATCGCCGGAAAAACGGCGCAACTGAGGCGTTCATTTTGGGCCCGGGAGCTGGGGCTGGGGTTGGTTGTGGTGTTGGGTTCGGGTTTGGGCTGGTAGGCGGAGCTGGGTTTTCTGGTGGGCTTTGGAATCACATGCATCTTGCTTTCGGGTTTGGGTACGGTTGCGGTGCGGGAGCTGGACTTGGATTTGGGCAGGGGTTGGGTTATGGTTCAAGTTGGAAAACGATTAAGTCGGACGTGCTTGGATTTGAGGAGTAA

Coding sequence (CDS)

ATGGATTCCAACTTCCTTCACAAAGTTGAACCCGTCGATTCTTTCTGGAACTGGAAACAGCATCAATCCCGCAAAAACAACAATATCAACAAATGGAAGTTTGAAGACAAACGGTTCTCTGCGCCGGAACTTTTCGGCACCAATCGCCGGAAAAACGGCGCAACTGAGGCGTTCATTTTGGGCCCGGGAGCTGGGGCTGGGGTTGGTTGTGGTGTTGGGTTCGGGTTTGGGCTGGTAGGCGGAGCTGGGTTTTCTGGTGGGCTTTGGAATCACATGCATCTTGCTTTCGGGTTTGGGTACGGTTGCGGTGCGGGAGCTGGACTTGGATTTGGGCAGGGGTTGGGTTATGGTTCAAGTTGGAAAACGATTAAGTCGGACGTGCTTGGATTTGAGGAGTAA

Protein sequence

MDSNFLHKVEPVDSFWNWKQHQSRKNNNINKWKFEDKRFSAPELFGTNRRKNGATEAFILGPGAGAGVGCGVGFGFGLVGGAGFSGGLWNHMHLAFGFGYGCGAGAGLGFGQGLGYGSSWKTIKSDVLGFEE

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo00222.1	Spo00222.1	mRNA

Homology

[Close]

BLAST of Spo00222.1 vs. NCBI nr
Match: gi|902196641|gb|KNA13162.1| (hypothetical protein SOVF_119290 [Spinacia oleracea])

HSP 1 Score: 270.4 bits (690), Expect = 1.700e-69
Identity = 132/132 (100.00%), Postives = 132/132 (100.00%), Query Frame = 1


		  
Query: 1   MDSNFLHKVEPVDSFWNWKQHQSRKNNNINKWKFEDKRFSAPELFGTNRRKNGATEAFIL 60
           MDSNFLHKVEPVDSFWNWKQHQSRKNNNINKWKFEDKRFSAPELFGTNRRKNGATEAFIL
Sbjct: 1   MDSNFLHKVEPVDSFWNWKQHQSRKNNNINKWKFEDKRFSAPELFGTNRRKNGATEAFIL 60

Query: 61  GPGAGAGVGCGVGFGFGLVGGAGFSGGLWNHMHLAFGFGYGCGAGAGLGFGQGLGYGSSW 120
           GPGAGAGVGCGVGFGFGLVGGAGFSGGLWNHMHLAFGFGYGCGAGAGLGFGQGLGYGSSW
Sbjct: 61  GPGAGAGVGCGVGFGFGLVGGAGFSGGLWNHMHLAFGFGYGCGAGAGLGFGQGLGYGSSW 120

Query: 121 KTIKSDVLGFEE 133
           KTIKSDVLGFEE
Sbjct: 121 KTIKSDVLGFEE 132

[Close]

BLAST of Spo00222.1 vs. NCBI nr
Match: gi|731315926|ref|XP_010693515.1| (PREDICTED: fibroin heavy chain [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 180.3 bits (456), Expect = 2.300e-42
Identity = 85/127 (66.93%), Postives = 107/127 (84.25%), Query Frame = 1


		  
Query: 6   LHKVEPVDSFWNWKQHQSRKNNNINKWKFEDKRFSAPELFGTNRRKNGATEAFILGPGAG 65
           LHKV+PVDSFWN++  Q +K NN+NKWK ++KRF+  ELF +NR++N + + F+LGPGAG
Sbjct: 15  LHKVDPVDSFWNFRT-QPKKTNNLNKWKVDEKRFAVTELFSSNRQRNASKDTFVLGPGAG 74

Query: 66  AGVGCGVGFGFGLVGGAGFSGGLWNHMHLAFGFGYGCGAGAGLGFGQGLGYGSSWKTIKS 125
           AGVGCGVG G GL GGAG+S GLWN + + FGFG+GCG GAG G+GQGLGYGSSWK+IKS
Sbjct: 75  AGVGCGVGLGLGLTGGAGYSFGLWNELRVVFGFGFGCGVGAGFGYGQGLGYGSSWKSIKS 134

Query: 126 DVLGFEE 133
           DVLGF++
Sbjct: 135 DVLGFKD 140

[Close]

BLAST of Spo00222.1 vs. NCBI nr
Match: gi|870867729|gb|KMT18598.1| (hypothetical protein BVRB_2g027290 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 110.9 bits (276), Expect = 1.700e-21
Identity = 64/117 (54.70%), Postives = 77/117 (65.81%), Query Frame = 1


		  
Query: 12  VDSFWNWKQHQSRKNNNINKWKFEDKRFSAPELFGTNRRKNGATEAFILGPGAGAGVGCG 71
           +DSFWN+KQ    K NN+N+W  +      P +F  +       E+FI   GAGAGVGCG
Sbjct: 14  LDSFWNYKQP---KKNNLNQWLDD----VMPTIFTWSNLWLVIRESFIHETGAGAGVGCG 73

Query: 72  VGFGFGLVGGAGFSGGLWNHMHLAFGFGYGCGAGAGLGFGQGLGYGSSWKTIKSDVL 129
           VGFGFGL GGAGFS G WN +    GFG+GCG G G G+GQGLGYGSSWK+I   +L
Sbjct: 74  VGFGFGLAGGAGFSDGPWNDLRAFSGFGFGCGVGVGFGYGQGLGYGSSWKSIGDALL 123

[Close]

BLAST of Spo00222.1 vs. NCBI nr
Match: gi|703146813|ref|XP_010108896.1| (hypothetical protein L484_027090 [Morus notabilis])

HSP 1 Score: 104.4 bits (259), Expect = 1.600e-19
Identity = 55/115 (47.83%), Postives = 75/115 (65.22%), Query Frame = 1


		  
Query: 13  DSFWNWKQHQSRKNNNINKWKFEDKRFSAPELFGTNRRKNGATEAFILGPGAGAGVGCGV 72
           ++ WNW +      + +NK +  +KRF   E    +R++    +  +LGPGAGAG+GCGV
Sbjct: 13  NALWNWNE------SKLNKKRNSEKRFPVAEFLDRSRQR---AQESVLGPGAGAGIGCGV 72

Query: 73  GFGFGLVGGAGFSGGLWNHMHLAFGFGYGCGAGAGLGFGQGLGYGSSWKTIKSDV 128
           G GFGLVGG GF G  WNH+ L  G G GCG G G+GFGQG GYG S ++++SD+
Sbjct: 73  GLGFGLVGGFGFGGPQWNHLKLVLGIGLGCGVGLGIGFGQGFGYGYSLESLESDL 118

[Close]

BLAST of Spo00222.1 vs. NCBI nr
Match: gi|590652501|ref|XP_007033168.1| (Uncharacterized protein TCM_019361 [Theobroma cacao])

HSP 1 Score: 103.6 bits (257), Expect = 2.700e-19
Identity = 57/121 (47.11%), Postives = 76/121 (62.81%), Query Frame = 1


		  
Query: 8   KVEPVDSFWNWKQHQSRKNNNINKWKFEDKRFSAPELFGTNRRKNGATEAFILGPGAGAG 67
           K +  +S W WK + ++K N         K+F   E    +R+K    E  +LGPG GAG
Sbjct: 4   KSKDTNSLWKWKGNMNKKKNG--------KQFPVSEFLRESRQK---VEENVLGPGGGAG 63

Query: 68  VGCGVGFGFGLVGGAGFSGGLWNHMHLAFGFGYGCGAGAGLGFGQGLGYGSSWKTIKSDV 127
           +GCG+G GFGLVGG G+ G  WNH+ LAFG G GCG G G GFGQG+GYG S ++++S +
Sbjct: 64  IGCGIGVGFGLVGGIGYGGWPWNHLKLAFGVGAGCGVGLGFGFGQGIGYGFSLESLESYM 113

Query: 128 L 129
           L
Sbjct: 124 L 113

[Close]

BLAST of Spo00222.1 vs. UniProtKB/TrEMBL
Match: A0A0K9R2Z5_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_119290 PE=4 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 1.200e-69
Identity = 132/132 (100.00%), Postives = 132/132 (100.00%), Query Frame = 1


		  
Query: 1   MDSNFLHKVEPVDSFWNWKQHQSRKNNNINKWKFEDKRFSAPELFGTNRRKNGATEAFIL 60
           MDSNFLHKVEPVDSFWNWKQHQSRKNNNINKWKFEDKRFSAPELFGTNRRKNGATEAFIL
Sbjct: 1   MDSNFLHKVEPVDSFWNWKQHQSRKNNNINKWKFEDKRFSAPELFGTNRRKNGATEAFIL 60

Query: 61  GPGAGAGVGCGVGFGFGLVGGAGFSGGLWNHMHLAFGFGYGCGAGAGLGFGQGLGYGSSW 120
           GPGAGAGVGCGVGFGFGLVGGAGFSGGLWNHMHLAFGFGYGCGAGAGLGFGQGLGYGSSW
Sbjct: 61  GPGAGAGVGCGVGFGFGLVGGAGFSGGLWNHMHLAFGFGYGCGAGAGLGFGQGLGYGSSW 120

Query: 121 KTIKSDVLGFEE 133
           KTIKSDVLGFEE
Sbjct: 121 KTIKSDVLGFEE 132

[Close]

BLAST of Spo00222.1 vs. UniProtKB/TrEMBL
Match: A0A0J8FSB1_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g027320 PE=4 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 1.600e-42
Identity = 85/127 (66.93%), Postives = 107/127 (84.25%), Query Frame = 1


		  
Query: 6   LHKVEPVDSFWNWKQHQSRKNNNINKWKFEDKRFSAPELFGTNRRKNGATEAFILGPGAG 65
           LHKV+PVDSFWN++  Q +K NN+NKWK ++KRF+  ELF +NR++N + + F+LGPGAG
Sbjct: 15  LHKVDPVDSFWNFRT-QPKKTNNLNKWKVDEKRFAVTELFSSNRQRNASKDTFVLGPGAG 74

Query: 66  AGVGCGVGFGFGLVGGAGFSGGLWNHMHLAFGFGYGCGAGAGLGFGQGLGYGSSWKTIKS 125
           AGVGCGVG G GL GGAG+S GLWN + + FGFG+GCG GAG G+GQGLGYGSSWK+IKS
Sbjct: 75  AGVGCGVGLGLGLTGGAGYSFGLWNELRVVFGFGFGCGVGAGFGYGQGLGYGSSWKSIKS 134

Query: 126 DVLGFEE 133
           DVLGF++
Sbjct: 135 DVLGFKD 140

[Close]

BLAST of Spo00222.1 vs. UniProtKB/TrEMBL
Match: A0A0J8D330_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g027290 PE=4 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.200e-21
Identity = 64/117 (54.70%), Postives = 77/117 (65.81%), Query Frame = 1


		  
Query: 12  VDSFWNWKQHQSRKNNNINKWKFEDKRFSAPELFGTNRRKNGATEAFILGPGAGAGVGCG 71
           +DSFWN+KQ    K NN+N+W  +      P +F  +       E+FI   GAGAGVGCG
Sbjct: 14  LDSFWNYKQP---KKNNLNQWLDD----VMPTIFTWSNLWLVIRESFIHETGAGAGVGCG 73

Query: 72  VGFGFGLVGGAGFSGGLWNHMHLAFGFGYGCGAGAGLGFGQGLGYGSSWKTIKSDVL 129
           VGFGFGL GGAGFS G WN +    GFG+GCG G G G+GQGLGYGSSWK+I   +L
Sbjct: 74  VGFGFGLAGGAGFSDGPWNDLRAFSGFGFGCGVGVGFGYGQGLGYGSSWKSIGDALL 123

[Close]

BLAST of Spo00222.1 vs. UniProtKB/TrEMBL
Match: W9S8Y9_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_027090 PE=4 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 1.100e-19
Identity = 55/115 (47.83%), Postives = 75/115 (65.22%), Query Frame = 1


		  
Query: 13  DSFWNWKQHQSRKNNNINKWKFEDKRFSAPELFGTNRRKNGATEAFILGPGAGAGVGCGV 72
           ++ WNW +      + +NK +  +KRF   E    +R++    +  +LGPGAGAG+GCGV
Sbjct: 13  NALWNWNE------SKLNKKRNSEKRFPVAEFLDRSRQR---AQESVLGPGAGAGIGCGV 72

Query: 73  GFGFGLVGGAGFSGGLWNHMHLAFGFGYGCGAGAGLGFGQGLGYGSSWKTIKSDV 128
           G GFGLVGG GF G  WNH+ L  G G GCG G G+GFGQG GYG S ++++SD+
Sbjct: 73  GLGFGLVGGFGFGGPQWNHLKLVLGIGLGCGVGLGIGFGQGFGYGYSLESLESDL 118

[Close]

BLAST of Spo00222.1 vs. UniProtKB/TrEMBL
Match: A0A0J8D2S8_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g027300 PE=4 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 1.900e-19
Identity = 61/121 (50.41%), Postives = 76/121 (62.81%), Query Frame = 1


		  
Query: 12  VDSFWNWKQHQSRKNNNINKWKFEDKRFSAPELFGTNRRKNGATEAFIL----GPGAGAG 71
           VDSFWN+KQ   +KN N  +W       +   +    +R    +E+ +     G G GAG
Sbjct: 7   VDSFWNYKQ--PKKNKNSYQWLD-----AMSSILTWTKRWFVISESLVYKTGAGAGVGAG 66

Query: 72  VGCGVGFGFGLVGGAGFSGGLWNHMHLAFGFGYGCGAGAGLGFGQGLGYGSSWKTIKSDV 129
           VGCGVGFG GL GGAGFS G WNH+ +  GFG+GCG G G G+GQGLGYGSSWK+I   +
Sbjct: 67  VGCGVGFGLGLAGGAGFSHGPWNHLRIFSGFGFGCGVGVGFGYGQGLGYGSSWKSIGDAL 120

[Close]

BLAST of Spo00222.1 vs. TAIR (Arabidopsis)
Match: AT1G66820.1 (glycine-rich protein)

HSP 1 Score: 54.7 bits (130), Expect = 5.100e-8
Identity = 35/67 (52.24%), Postives = 43/67 (64.18%), Query Frame = 1


		  
Query: 60  LGPGAGAGVGCGVGFGFGLVGGAGF--SGGLWNHMHLAFGFGYGCGAGAGLGFGQGLGYG 119
           +GPG G G+GCG G G GL GG G   S GL +H ++  GFG GCG G G G+G G+G G
Sbjct: 39  VGPGIGGGIGCGAGIGIGLSGGLGIGASEGL-DHSNVVLGFGIGCGIGFGFGYGFGVGGG 98

Query: 120 SSWKTIK 125
            S+  IK
Sbjct: 99  YSFDDIK 104

[Close]

BLAST of Spo00222.1 vs. TAIR (Arabidopsis)
Match: AT4G10330.1 (glycine-rich protein)

HSP 1 Score: 54.3 bits (129), Expect = 6.600e-8
Identity = 33/66 (50.00%), Postives = 43/66 (65.15%), Query Frame = 1


		  
Query: 60  LGPGAGAGVGCGVGFGFGLVGGAGFSGGLWNHMHLAFGFGYGCGAGAGLGFGQGLGYGSS 119
           +GP  G GVGCG GFG GL+GG GF  G+     L FG G+G G G G+GFG G+G G++
Sbjct: 29  VGPAFGLGVGCGFGFGAGLIGGVGFGPGV---PGLQFGLGFGAGCGIGVGFGYGVGRGAA 88

Query: 120 WKTIKS 126
           +   +S
Sbjct: 89  YDHSRS 91

The following BLAST results are available for this feature:

BLAST of Spo00222.1 vs. NCBI nr
BLAST of Spo00222.1 vs. UniProtKB/TrEMBL
BLAST of Spo00222.1 vs. ExPASy Swiss-Prot
BLAST of Spo00222.1 vs. TAIR (Arabidopsis)

BLAST of Spo00222.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902196641\|gb\|KNA13162.1\|	1.7e-69	100.	hypothetical protein SOVF_1192...	[more]
gi\|731315926\|ref\|XP_010693515.1\|	2.3e-42	66.9	PREDICTED: fibroin heavy chain...	[more]
gi\|870867729\|gb\|KMT18598.1\|	1.7e-21	54.7	hypothetical protein BVRB_2g02...	[more]
gi\|703146813\|ref\|XP_010108896.1\|	1.6e-19	47.8	hypothetical protein L484_0270...	[more]
gi\|590652501\|ref\|XP_007033168.1\|	2.7e-19	47.1	Uncharacterized protein TCM_01...	[more]

BLAST of Spo00222.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9R2Z5_SPIOL	1.2e-69	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8FSB1_BETVU	1.6e-42	66.9	Uncharacterized protein OS=Bet...	[more]
A0A0J8D330_BETVU	1.2e-21	54.7	Uncharacterized protein OS=Bet...	[more]
W9S8Y9_9ROSA	1.1e-19	47.8	Uncharacterized protein OS=Mor...	[more]
A0A0J8D2S8_BETVU	1.9e-19	50.4	Uncharacterized protein OS=Bet...	[more]

BLAST of Spo00222.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Spo00222.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 2


Match Name	E-value	Identity	Description
AT1G66820.1	5.1e-8	52.2	glycine-rich protein	[more]
AT4G10330.1	6.6e-8	50.0	glycine-rich protein	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR34201	FAMILY NOT NAMED	coord: 13..125 score: 1.3
None	No IPR available	PANTHER	PTHR34201:SF2	GLYCINE-RICH PROTEIN	coord: 13..125 score: 1.3

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo01868	0.74	Barchart \| Table
Spo17628	0.74	Barchart \| Table
Spo12742	0.74	Barchart \| Table
Spo24440	0.73	Barchart \| Table
Spo02915	0.73	Barchart \| Table
Spo16321	0.72	Barchart \| Table
Spo19822	0.72	Barchart \| Table
Spo15850	0.71	Barchart \| Table
Spo22528	0.71	Barchart \| Table
Spo19196	0.71	Barchart \| Table
Spo27330	0.70	Barchart \| Table
Spo20966	0.70	Barchart \| Table
Spo16817	0.69	Barchart \| Table
Spo05752	0.69	Barchart \| Table
Spo26661	0.69	Barchart \| Table
Spo11493	0.68	Barchart \| Table
Spo14333	0.68	Barchart \| Table
Spo14697	0.68	Barchart \| Table
Spo21542	0.68	Barchart \| Table
Spo15428	0.68	Barchart \| Table
Spo10752	0.68	Barchart \| Table
Spo07718	0.68	Barchart \| Table
Spo26850	0.68	Barchart \| Table
Spo16568	0.68	Barchart \| Table
Spo04884	0.68	Barchart \| Table
Spo19195	0.68	Barchart \| Table
Spo06829	0.67	Barchart \| Table
Spo04763	0.67	Barchart \| Table
Spo06115	0.67	Barchart \| Table
Spo18834	0.67	Barchart \| Table
Spo20806	0.67	Barchart \| Table
Spo22732	0.67	Barchart \| Table
Spo23932	0.67	Barchart \| Table
Spo20628	0.66	Barchart \| Table
Spo08205	0.66	Barchart \| Table
Spo15429	0.66	Barchart \| Table
Spo14857	0.66	Barchart \| Table
Spo14130	0.66	Barchart \| Table
Spo07623	0.66	Barchart \| Table
Spo22876	0.66	Barchart \| Table
Spo06881	0.66	Barchart \| Table
Spo10798	0.66	Barchart \| Table
Spo26195	0.66	Barchart \| Table
Spo18746	0.66	Barchart \| Table
Spo16806	0.66	Barchart \| Table
Spo24050	0.65	Barchart \| Table
Spo17803	0.65	Barchart \| Table
Spo25351	0.65	Barchart \| Table
Spo25873	0.65	Barchart \| Table