Spo00320 (gene) | SpinachBase

Overview


Name	Spo00320
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Auxin transport protein BIG
Location	SpoScf_01756 : 51563 .. 51847 (+)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTAGCCTGGCGATATCTTCAAGATTGCTCCAGGTTCCATTCCCAAAGCAAACTTTGTTGGCTACCGATGATGCGGTAGACAATGCTACTTCAGCAGCTGCTCATGCTGATGCAGTGACCGCATCTGTAGGCAATGCACAGGTCATGATGGAAGAAGATTCTATTACATCCTCAGTACAATATTGTTGCTCCACTGTTCCTATACTAAGGCGAAGGTGGCATTGCACCATTTGCTCAGATTTAGATGGCTTCCTCCACTACATTCCTGAGATCATCCAATGA

mRNA sequence

ATGAGTAGCCTGGCGATATCTTCAAGATTGCTCCAGGTTCCATTCCCAAAGCAAACTTTGTTGGCTACCGATGATGCGGTAGACAATGCTACTTCAGCAGCTGCTCATGCTGATGCAGTGACCGCATCTGTAGGCAATGCACAGGTCATGATGGAAGAAGATTCTATTACATCCTCAGTACAATATTGTTGCTCCACTGTTCCTATACTAAGGCGAAGGTGGCATTGCACCATTTGCTCAGATTTAGATGGCTTCCTCCACTACATTCCTGAGATCATCCAATGA

Coding sequence (CDS)

ATGAGTAGCCTGGCGATATCTTCAAGATTGCTCCAGGTTCCATTCCCAAAGCAAACTTTGTTGGCTACCGATGATGCGGTAGACAATGCTACTTCAGCAGCTGCTCATGCTGATGCAGTGACCGCATCTGTAGGCAATGCACAGGTCATGATGGAAGAAGATTCTATTACATCCTCAGTACAATATTGTTGCTCCACTGTTCCTATACTAAGGCGAAGGTGGCATTGCACCATTTGCTCAGATTTAGATGGCTTCCTCCACTACATTCCTGAGATCATCCAATGA

Protein sequence

MSSLAISSRLLQVPFPKQTLLATDDAVDNATSAAAHADAVTASVGNAQVMMEEDSITSSVQYCCSTVPILRRRWHCTICSDLDGFLHYIPEIIQ

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo00320.1	Spo00320.1	mRNA

Homology

[Close]

BLAST of Spo00320.1 vs. NCBI nr
Match: gi|902170058|gb|KNA07772.1| (hypothetical protein SOVF_168850 isoform B [Spinacia oleracea])

HSP 1 Score: 184.9 bits (468), Expect = 6.600e-44
Identity = 93/94 (98.94%), Postives = 94/94 (100.00%), Query Frame = 1


		  
Query: 1  MSSLAISSRLLQVPFPKQTLLATDDAVDNATSAAAHADAVTASVGNAQVMMEEDSITSSV 60
          MSSLAISSRLLQVPFPKQTLLATDDAVDNATSAAAHADAVTASVGNAQVMMEEDSITSSV
Sbjct: 1  MSSLAISSRLLQVPFPKQTLLATDDAVDNATSAAAHADAVTASVGNAQVMMEEDSITSSV 60

Query: 61 QYCCSTVPILRRRWHCTICSDLDGFLHYIPEIIQ 95
          QYCCSTVPILRRRWHCTICSDLDGFLH+IPEIIQ
Sbjct: 61 QYCCSTVPILRRRWHCTICSDLDGFLHHIPEIIQ 94

[Close]

BLAST of Spo00320.1 vs. NCBI nr
Match: gi|902170057|gb|KNA07771.1| (hypothetical protein SOVF_168850 isoform A [Spinacia oleracea])

HSP 1 Score: 183.0 bits (463), Expect = 2.500e-43
Identity = 92/93 (98.92%), Postives = 93/93 (100.00%), Query Frame = 1


		  
Query: 2   SSLAISSRLLQVPFPKQTLLATDDAVDNATSAAAHADAVTASVGNAQVMMEEDSITSSVQ 61
           SSLAISSRLLQVPFPKQTLLATDDAVDNATSAAAHADAVTASVGNAQVMMEEDSITSSVQ
Sbjct: 53  SSLAISSRLLQVPFPKQTLLATDDAVDNATSAAAHADAVTASVGNAQVMMEEDSITSSVQ 112

Query: 62  YCCSTVPILRRRWHCTICSDLDGFLHYIPEIIQ 95
           YCCSTVPILRRRWHCTICSDLDGFLH+IPEIIQ
Sbjct: 113 YCCSTVPILRRRWHCTICSDLDGFLHHIPEIIQ 145

[Close]

BLAST of Spo00320.1 vs. NCBI nr
Match: gi|902193211|gb|KNA12234.1| (hypothetical protein SOVF_127340 [Spinacia oleracea])

HSP 1 Score: 147.9 bits (372), Expect = 8.900e-33
Identity = 77/85 (90.59%), Postives = 78/85 (91.76%), Query Frame = 1


		  
Query: 2    SSLAISSRLLQVPFPKQTLLATDDAVDNATSAAAHADAVTASVGNAQVMMEEDSITSSVQ 61
            SSLAISSRLLQVPFPKQT+LATDDA DNATSAAAH DAVTASVGNAQVMMEEDSITSSVQ
Sbjct: 2355 SSLAISSRLLQVPFPKQTMLATDDAADNATSAAAHPDAVTASVGNAQVMMEEDSITSSVQ 2414

Query: 62   YC---CSTVPILRRRWHCTICSDLD 84
            YC   CSTVPILRRRWHCTIC D D
Sbjct: 2415 YCCDGCSTVPILRRRWHCTICPDFD 2439

[Close]

BLAST of Spo00320.1 vs. NCBI nr
Match: gi|469469090|gb|AGH32778.1| (auxin transport protein BIG [Krascheninnikovia arborescens])

HSP 1 Score: 142.5 bits (358), Expect = 3.700e-31
Identity = 74/85 (87.06%), Postives = 75/85 (88.24%), Query Frame = 1


		  
Query: 2    SSLAISSRLLQVPFPKQTLLATDDAVDNATSAAAHADAVTASVGNAQVMMEEDSITSSVQ 61
            SSLAISSRLLQVPFPKQT+LATDDA DNA SA AH DAVTAS GNAQVMMEEDSITSSVQ
Sbjct: 2536 SSLAISSRLLQVPFPKQTMLATDDAADNAASAPAHPDAVTASAGNAQVMMEEDSITSSVQ 2595

Query: 62   YC---CSTVPILRRRWHCTICSDLD 84
            YC   CSTVPILRRRWHCTIC D D
Sbjct: 2596 YCCDGCSTVPILRRRWHCTICPDFD 2620

[Close]

BLAST of Spo00320.1 vs. NCBI nr
Match: gi|870867827|gb|KMT18696.1| (hypothetical protein BVRB_2g028210 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 139.4 bits (350), Expect = 3.200e-30
Identity = 70/85 (82.35%), Postives = 76/85 (89.41%), Query Frame = 1


		  
Query: 2    SSLAISSRLLQVPFPKQTLLATDDAVDNATSAAAHADAVTASVGNAQVMMEEDSITSSVQ 61
            SSLAISSRLLQVPFPKQT+LATDDA DNATSA+AH D VTAS GNAQVMMEE+++TSSVQ
Sbjct: 2011 SSLAISSRLLQVPFPKQTMLATDDAADNATSASAHVDTVTASGGNAQVMMEEETVTSSVQ 2070

Query: 62   YC---CSTVPILRRRWHCTICSDLD 84
            YC   CSTVPILRRRWHCT+C D D
Sbjct: 2071 YCCDGCSTVPILRRRWHCTVCPDFD 2095

[Close]

BLAST of Spo00320.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QKK0_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_168850 PE=4 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 4.600e-44
Identity = 93/94 (98.94%), Postives = 94/94 (100.00%), Query Frame = 1


		  
Query: 1  MSSLAISSRLLQVPFPKQTLLATDDAVDNATSAAAHADAVTASVGNAQVMMEEDSITSSV 60
          MSSLAISSRLLQVPFPKQTLLATDDAVDNATSAAAHADAVTASVGNAQVMMEEDSITSSV
Sbjct: 1  MSSLAISSRLLQVPFPKQTLLATDDAVDNATSAAAHADAVTASVGNAQVMMEEDSITSSV 60

Query: 61 QYCCSTVPILRRRWHCTICSDLDGFLHYIPEIIQ 95
          QYCCSTVPILRRRWHCTICSDLDGFLH+IPEIIQ
Sbjct: 61 QYCCSTVPILRRRWHCTICSDLDGFLHHIPEIIQ 94

[Close]

BLAST of Spo00320.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QMH5_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_168850 PE=4 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 1.700e-43
Identity = 92/93 (98.92%), Postives = 93/93 (100.00%), Query Frame = 1


		  
Query: 2   SSLAISSRLLQVPFPKQTLLATDDAVDNATSAAAHADAVTASVGNAQVMMEEDSITSSVQ 61
           SSLAISSRLLQVPFPKQTLLATDDAVDNATSAAAHADAVTASVGNAQVMMEEDSITSSVQ
Sbjct: 53  SSLAISSRLLQVPFPKQTLLATDDAVDNATSAAAHADAVTASVGNAQVMMEEDSITSSVQ 112

Query: 62  YCCSTVPILRRRWHCTICSDLDGFLHYIPEIIQ 95
           YCCSTVPILRRRWHCTICSDLDGFLH+IPEIIQ
Sbjct: 113 YCCSTVPILRRRWHCTICSDLDGFLHHIPEIIQ 145

[Close]

BLAST of Spo00320.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QY85_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_127340 PE=4 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 6.200e-33
Identity = 77/85 (90.59%), Postives = 78/85 (91.76%), Query Frame = 1


		  
Query: 2    SSLAISSRLLQVPFPKQTLLATDDAVDNATSAAAHADAVTASVGNAQVMMEEDSITSSVQ 61
            SSLAISSRLLQVPFPKQT+LATDDA DNATSAAAH DAVTASVGNAQVMMEEDSITSSVQ
Sbjct: 2355 SSLAISSRLLQVPFPKQTMLATDDAADNATSAAAHPDAVTASVGNAQVMMEEDSITSSVQ 2414

Query: 62   YC---CSTVPILRRRWHCTICSDLD 84
            YC   CSTVPILRRRWHCTIC D D
Sbjct: 2415 YCCDGCSTVPILRRRWHCTICPDFD 2439

[Close]

BLAST of Spo00320.1 vs. UniProtKB/TrEMBL
Match: M4QUG0_9CARY (Auxin transport protein BIG OS=Krascheninnikovia arborescens GN=BIG PE=2 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 2.600e-31
Identity = 74/85 (87.06%), Postives = 75/85 (88.24%), Query Frame = 1


		  
Query: 2    SSLAISSRLLQVPFPKQTLLATDDAVDNATSAAAHADAVTASVGNAQVMMEEDSITSSVQ 61
            SSLAISSRLLQVPFPKQT+LATDDA DNA SA AH DAVTAS GNAQVMMEEDSITSSVQ
Sbjct: 2536 SSLAISSRLLQVPFPKQTMLATDDAADNAASAPAHPDAVTASAGNAQVMMEEDSITSSVQ 2595

Query: 62   YC---CSTVPILRRRWHCTICSDLD 84
            YC   CSTVPILRRRWHCTIC D D
Sbjct: 2596 YCCDGCSTVPILRRRWHCTICPDFD 2620

[Close]

BLAST of Spo00320.1 vs. UniProtKB/TrEMBL
Match: A0A0J8FSK4_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g028210 PE=4 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 2.200e-30
Identity = 70/85 (82.35%), Postives = 76/85 (89.41%), Query Frame = 1


		  
Query: 2    SSLAISSRLLQVPFPKQTLLATDDAVDNATSAAAHADAVTASVGNAQVMMEEDSITSSVQ 61
            SSLAISSRLLQVPFPKQT+LATDDA DNATSA+AH D VTAS GNAQVMMEE+++TSSVQ
Sbjct: 2011 SSLAISSRLLQVPFPKQTMLATDDAADNATSASAHVDTVTASGGNAQVMMEEETVTSSVQ 2070

Query: 62   YC---CSTVPILRRRWHCTICSDLD 84
            YC   CSTVPILRRRWHCT+C D D
Sbjct: 2071 YCCDGCSTVPILRRRWHCTVCPDFD 2095

[Close]

BLAST of Spo00320.1 vs. ExPASy Swiss-Prot
Match: BIG_ORYSJ (Auxin transport protein BIG OS=Oryza sativa subsp. japonica GN=Os09g0247700 PE=2 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 5.800e-24
Identity = 59/85 (69.41%), Postives = 66/85 (77.65%), Query Frame = 1


		  
Query: 2    SSLAISSRLLQVPFPKQTLLATDDAVDNATSAAAHADAVTASVGNAQVMMEEDSITSSVQ 61
            SSLAISSR LQVPFPKQT++A DDA DN   A+A +++ T   GNAQVM+EED  TSSVQ
Sbjct: 2415 SSLAISSRFLQVPFPKQTMIANDDAPDNHAKASAASNSTT---GNAQVMIEEDPATSSVQ 2474

Query: 62   YC---CSTVPILRRRWHCTICSDLD 84
            YC   CSTVPILRRRWHC IC D D
Sbjct: 2475 YCCDGCSTVPILRRRWHCNICPDFD 2496

[Close]

BLAST of Spo00320.1 vs. ExPASy Swiss-Prot
Match: BIG_ARATH (Auxin transport protein BIG OS=Arabidopsis thaliana GN=BIG PE=1 SV=2)

HSP 1 Score: 110.2 bits (274), Expect = 1.300e-23
Identity = 57/83 (68.67%), Postives = 64/83 (77.11%), Query Frame = 1


		  
Query: 4    LAISSRLLQVPFPKQTLLATDDAVDNATSAAAHADAVTASVGNAQVMMEEDSITSSVQYC 63
            LAISSRLLQVPFPKQT+L TDD VDN T+ +     +  + GN  VM+EEDSITSSVQYC
Sbjct: 2561 LAISSRLLQVPFPKQTMLTTDDLVDNVTTPSV---PIRTAGGNTHVMIEEDSITSSVQYC 2620

Query: 64   ---CSTVPILRRRWHCTICSDLD 84
               CSTVPILRRRWHCT+C D D
Sbjct: 2621 CDGCSTVPILRRRWHCTVCPDFD 2640

[Close]

BLAST of Spo00320.1 vs. TAIR (Arabidopsis)
Match: AT3G02260.1 (auxin transport protein (BIG))

HSP 1 Score: 110.2 bits (274), Expect = 7.200e-25
Identity = 57/83 (68.67%), Postives = 64/83 (77.11%), Query Frame = 1


		  
Query: 4    LAISSRLLQVPFPKQTLLATDDAVDNATSAAAHADAVTASVGNAQVMMEEDSITSSVQYC 63
            LAISSRLLQVPFPKQT+L TDD VDN T+ +     +  + GN  VM+EEDSITSSVQYC
Sbjct: 2561 LAISSRLLQVPFPKQTMLTTDDLVDNVTTPSV---PIRTAGGNTHVMIEEDSITSSVQYC 2620

Query: 64   ---CSTVPILRRRWHCTICSDLD 84
               CSTVPILRRRWHCT+C D D
Sbjct: 2621 CDGCSTVPILRRRWHCTVCPDFD 2640

The following BLAST results are available for this feature:

BLAST of Spo00320.1 vs. NCBI nr
BLAST of Spo00320.1 vs. UniProtKB/TrEMBL
BLAST of Spo00320.1 vs. ExPASy Swiss-Prot
BLAST of Spo00320.1 vs. TAIR (Arabidopsis)

BLAST of Spo00320.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902170058\|gb\|KNA07772.1\|	6.6e-44	98.9	hypothetical protein SOVF_1688...	[more]
gi\|902170057\|gb\|KNA07771.1\|	2.5e-43	98.9	hypothetical protein SOVF_1688...	[more]
gi\|902193211\|gb\|KNA12234.1\|	8.9e-33	90.5	hypothetical protein SOVF_1273...	[more]
gi\|469469090\|gb\|AGH32778.1\|	3.7e-31	87.0	auxin transport protein BIG [K...	[more]
gi\|870867827\|gb\|KMT18696.1\|	3.2e-30	82.3	hypothetical protein BVRB_2g02...	[more]

BLAST of Spo00320.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9QKK0_SPIOL	4.6e-44	98.9	Uncharacterized protein OS=Spi...	[more]
A0A0K9QMH5_SPIOL	1.7e-43	98.9	Uncharacterized protein OS=Spi...	[more]
A0A0K9QY85_SPIOL	6.2e-33	90.5	Uncharacterized protein OS=Spi...	[more]
M4QUG0_9CARY	2.6e-31	87.0	Auxin transport protein BIG OS...	[more]
A0A0J8FSK4_BETVU	2.2e-30	82.3	Uncharacterized protein OS=Bet...	[more]

BLAST of Spo00320.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 2


Match Name	E-value	Identity	Description
BIG_ORYSJ	5.8e-24	69.4	Auxin transport protein BIG OS...	[more]
BIG_ARATH	1.3e-23	68.6	Auxin transport protein BIG OS...	[more]

BLAST of Spo00320.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1


Match Name	E-value	Identity	Description
AT3G02260.1	7.2e-25	68.6	auxin transport protein (BIG)	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR21725	PUSHOVER/RETINOBLASTOMA-ASSOCIATED FACTOR 600	coord: 2..83 score: 2.6
None	No IPR available	PANTHER	PTHR21725:SF1	E3 UBIQUITIN-PROTEIN LIGASE UBR4	coord: 2..83 score: 2.6
None	No IPR available	unknown	SSF57850	RING/U-box	coord: 48..83 score: 8.

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0008270	zinc ion binding

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo26726	0.67	Barchart \| Table