Spo00478 (gene) | SpinachBase

Overview


Name	Spo00478
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Uroporphyrinogen-III C-methyltransferase
Location	SpoScf_01216 : 130176 .. 134470 (+)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AGACTAGTAAAGTTCCATAGACCATAGGTTTCCAAATTTGATTTTAGCATCCTTTTGTTTATGTTGGATGAACCTGTCCCTTATATGGGCTAAAAGGTCACCCACCGCCTTAACTTTGGCTTTCCATCTTCCCCAAAGTCTACTGCTGCGGCTGCTTGGCTGGCTGCCCTTTGCCACCACCACCAACATACATGAACCATTACACCATTTTTTCACCATTCTCATCATTCCTCATAAAACCCCATTCAATCGTTATTGTACAACACCATTGTTGAAAGCTACCCTTTTCAATGGCTTCTCTAAATCATCTTCATTCATTATCAATTCAAACTTCCACCGCATTTCATACCAATCTGCAAAAACCCAAAACTTTTCACACCCAAATCTGCGTTAATTATAGGCAAATTGTATGTTGTTCAACTTCTTCACCCTTCACTGAGAAGAATTCGGTGGAGCGATACCAAAGGGAGCACTGGGTTTATCCTGTTATATCTCAATTTCAATCATCTCAATCTCAAGAACACTGTCAATCGTCTTCGCCGCCGCAGCCGTCTAAGGATAGAAGGGAAGATGACATTGCTTCCCAACTACCAGAACTCAAGAAGCTGCTTGAGGTATTGAGAGAAAAGAGAGAGAGCCAATCACAAGGTGGCGGCAATTCTGGTGTTATTGGAAATGTGTATTTGGTGGGCACTGGTCCAGGGGACCCTGAGTTACTCACTGTAAAAGCTCTCAAAATAATTCAATCTGCTGATCTTCTATTGTACGATCGCCTCGTCTCGAATGATGTGTTGGAAATGGTGGGCCCCAATGCTAAGCTTCTGTATGTTGGGAAGACTGCTGGCTACCATAGTAGGACACAGGAGGAGATTCATCAGCTGCTGTTGAGTTTTGCTGAAGCTGGTGCTAATGTTGTTAGACTTAAAGGCGGAGATCCTCTTGTAAGTAATGTTTATGTGCTAACAATGACTATTTGTTTGTGTCTACGATTGAGTAAAGCATATCACTGTGGTTTATTTTGTGTGATGTTGTGCTAGATTTTCGGACGCGGTGGCGAAGAGATGGATTTCCTGCAGCAGAAGGGGATTCAAGTTAAAGTTGTTCCAGGTACTGCTCTATTAGTTGTTGTTGAATGTACTAACTACAAGTGTTGATCTTTAATTCTTTCGAATGTTACAAGAGCACATAATGACATACGTTAACTTAGTTAATTAATATTGCAGAAAGCATATAAACCTGTTTAGTTTTTATATCAAAGTATTGGAAGGAATAATGCGGGAAGAATTGTAGTATAAGTTGGAATCAGGTTTCTAACTGTGACAGATTAGTGTGCAAGAGGAGTAAAGGGTGAGTGTCTTGATCATTAGCTTTTACTTGCCTCCCTGTGTCTACCAATAGGTCAGGTTTTGTTAGTTTTTTTGCTGTGTTTAGCTGTAGGGTCGAATATGTTATCGGCCTGAACTTCAGGGTGCACTGTCAGAATAACTCTTTCCTTACTCTGCAATTATCTTTGACTTGAAGTGGAATTACTACTCATGAGAAAATGGTGGGTTTTGTACTTATTCTGCGAGCTTCTTGATGTTACCTTTATGTATAAATTAAACTACCTTTCCAGATATAATTGATGTTTCCTTTATGTACAACTATAATATGAAAGTACTTGTCGAGATATAATTTAAACTACCTTTGTGGACATTTAGTTTTCACATTACTAGTCTAATGAAAATGTCATACTTCAATGCATAAGCTCTCTGCTTTTATTTGTCATACTTCAATACACAAGCCTATTTTTATCAAACTTTGTATTGTGCTCGTCATTGAGTTGTACTATGTCGTGCTTCATAGGTATAACACAGTGATGCATTATGACTTTCTTGCAATAAACTAAAATTTCCATTTATCATTGCAATAAAACCACAGCAACCAAAGCATCTGTTATTCGTATAATCGTATGAAGCATAGAAATGTTTCTAGTAGTATCTAAGGATAACTCATCCATAGAGCAATCAATGTAGTACTATTTTATTTAATTTCCCATCAGGACCTGCTTCATTCTGCACATTTCTCTTGGAATGTATCTTTATTCATACTTCATATTTTATCGTGAACTAGAAAAAATAGTTGCTCAAAGCATCAATGTTTAGTAAACTCCACTTTCTAGGAAGGGCTAGAACACCAACCCTTCATATGCAACAACTAAATTACATAACAAGCAACTCATCTCATCAAAACTTCTACAGGCAACTTTAAACACAATAGACCTTAACAAATTTCTACTGTTAACAAGGCAATTGTTGAAGATCTTCTGGTTCCGTCAAAGCTAAATGTTGTAAACACATTGCAATTTATGATCTGGCTTTTTACTCATGCACCCTTTTGCGGCCCGAAGAACTTCTAATTGGAAGTCATCAATTGATCTATTGCACCTGAGCATATGCAAACATTTACTCCAAACATTTGTTGAAATAGTAGCAAGCAAAGAAAAGATGAGAAATGTTCTCATCAGCTTGTCCACACAGGACACAAGTACCATCTGTTGCCACATTCCACCTTTGAGTTCTGTCGTTAGGTAGTTAATAGTTAACTTATTAAGAATTGCAAGCCAAGTAATAAAGATACTCCTTGTACTGGACATAATATTGCACTAACTTGTATATACAAATAGTTATATAAAATCATAAGTGTCCCTTTAGATAATGTGATACGCAGTACTTGTATAAAGTCATTCTCAGTCTCTATAACAAAGAAAAAGTAGTTAGTTCTCAGTCGTTATATGTTATCATAAGGGTCCCTTTGGTTTCTCACCTGTTCTCCCTTTGCCACGCCCTATGAAAAAGTTGCTTTACTTTAATACTGACTAAATGCTGAAGCAAATATTATTTACCAGCTGAAATTTTCTTCCTTGTAAGGTATTACTGCTGCTTCAGGTATATCAGCTGAACTAGGAATTCCGTTGACTCATCGAGGCATCGCAACTAGTGTTAGATATCTCACTGGACACTCAAGGAATGGGGGAACTGATCCGTTGTATGTAGCAGAGAATGCTGCTGACCCTGACTGTACCCTAGTTGTATATATGGGTTTGGCAACACTTCCTGCTCTTGCCCAGAAATTGGTGCAACATGGCCTGCCTAATGATACTCCTGCTGTTGCAGTGGAAAGAGGGACAACTGCTCAACAGCGTTTGGTAAGGAATACCTAACCGTTCCTTGATTCAATTTTGTTTATAATTTTTATATACTGTCTGCATTGGCATTACATAATTTGAGGTGTAGATTGGTAGGGATTTTTATTTCAAGGCTTGAACACATTTGTTATCTTTCTATGATTTCTGCACCTCCTCCATTATAATCAGCAAGTCAATAAAAAAGAAAAGGGGTGTGATCTGATGTGGAAGAGTTGAGACAACCCATAGACTGTTACCTTTTCTTTTGATCTCAAACAACCGAGCTCTGATTAAAATTCAGAAAATTCCTCAGTCGAATACCATTACTTCTATTCTGAATTCGTCACTAAAGCTGCATAAAATTATCAGAAAGGAGCCAAGGCAAAGCGTTTGCAGACGGAGCAACACTCAGCAGATCTTCCCACATTTTCTTCCTGGTTTCAACTGTATGCAGGCATAAATTGCACCAAACAAAGAAAGCAAAATGTTCCCTTGATTGTCATAGAGCTTACATTGAGTAAACTGCTAATGGTCTTGAAGGACAGTACAATAACATTAACCTCTAAGTGATTGGGCCAAATTCTCCTCTTATGAGAATGGGAGTAAGCAGATCCATTTCCAATGCTGACCAAACTTGCACATAATTTTTGGACATGTTTCAAGTTTCAACTAGGGCTTTGGGATACATTTATAAGATCATATTTTGTTTCTAATGAAGGAATGCTGGAATTAGTGGAAATGCCAATCATGTATTCAATTGTATTATATATAGTACTCACTTTACCTCTGTCCTGGAATGAATTATCGGCTACCTTTTAGAGTAGGATCTGGATAGTCCCTTGCCTTTTATATTGAGTTTGTCTTGTCATAAAATAGTAATATGAGGTAAATTTGGGTAATGTGATTTCTATTCATACAACGAACTCAACTAGGGAGGCACATTTTAGATTACTGTGTGACTAGATTCTCATTTGAACAGGTATTTTCAAAGTTGGAGAATCTTGCTGATAAAATTAGATGTAGTGGACTGGTATCCCCTACACTGATCATCATCGGGAAGGTTGTTGCACTTTCCCCATTGTGGATGCAATCCTGTGAAGAACCAGCTGAGCTTGTGGATGCAAAGTAG

mRNA sequence

AGACTAGTAAAGTTCCATAGACCATAGGTTTCCAAATTTGATTTTAGCATCCTTTTGTTTATGTTGGATGAACCTGTCCCTTATATGGGCTAAAAGGTCACCCACCGCCTTAACTTTGGCTTTCCATCTTCCCCAAAGTCTACTGCTGCGGCTGCTTGGCTGGCTGCCCTTTGCCACCACCACCAACATACATGAACCATTACACCATTTTTTCACCATTCTCATCATTCCTCATAAAACCCCATTCAATCGTTATTGTACAACACCATTGTTGAAAGCTACCCTTTTCAATGGCTTCTCTAAATCATCTTCATTCATTATCAATTCAAACTTCCACCGCATTTCATACCAATCTGCAAAAACCCAAAACTTTTCACACCCAAATCTGCGTTAATTATAGGCAAATTGTATGTTGTTCAACTTCTTCACCCTTCACTGAGAAGAATTCGGTGGAGCGATACCAAAGGGAGCACTGGGTTTATCCTGTTATATCTCAATTTCAATCATCTCAATCTCAAGAACACTGTCAATCGTCTTCGCCGCCGCAGCCGTCTAAGGATAGAAGGGAAGATGACATTGCTTCCCAACTACCAGAACTCAAGAAGCTGCTTGAGGTATTGAGAGAAAAGAGAGAGAGCCAATCACAAGGTGGCGGCAATTCTGGTGTTATTGGAAATGTGTATTTGGTGGGCACTGGTCCAGGGGACCCTGAGTTACTCACTGTAAAAGCTCTCAAAATAATTCAATCTGCTGATCTTCTATTGTACGATCGCCTCGTCTCGAATGATGTGTTGGAAATGGTGGGCCCCAATGCTAAGCTTCTGTATGTTGGGAAGACTGCTGGCTACCATAGTAGGACACAGGAGGAGATTCATCAGCTGCTGTTGAGTTTTGCTGAAGCTGGTGCTAATGTTGTTAGACTTAAAGGCGGAGATCCTCTTATTTTCGGACGCGGTGGCGAAGAGATGGATTTCCTGCAGCAGAAGGGGATTCAAGTTAAAGTTGTTCCAGGTATTACTGCTGCTTCAGGTATATCAGCTGAACTAGGAATTCCGTTGACTCATCGAGGCATCGCAACTAGTGTTAGATATCTCACTGGACACTCAAGGAATGGGGGAACTGATCCGTTGTATGTAGCAGAGAATGCTGCTGACCCTGACTGTACCCTAGTTGTATATATGGGTTTGGCAACACTTCCTGCTCTTGCCCAGAAATTGGTGCAACATGGCCTGCCTAATGATACTCCTGCTGTTGCAGTGGAAAGAGGGACAACTGCTCAACAGCGTTTGAAAGGAGCCAAGGCAAAGCGTTTGCAGACGGAGCAACACTCAGCAGATCTTCCCACATTTTCTTCCTGGTTTCAACTGTATGCAGGCATAAATTGCACCAAACAAAGAAAGCAAAATGTTCCCTTGATTGTCATAGAGCTTACATTGAGTAAACTGCTAATGGTATTTTCAAAGTTGGAGAATCTTGCTGATAAAATTAGATGTAGTGGACTGGTATCCCCTACACTGATCATCATCGGGAAGGTTGTTGCACTTTCCCCATTGTGGATGCAATCCTGTGAAGAACCAGCTGAGCTTGTGGATGCAAAGTAG

Coding sequence (CDS)

ATGGCTTCTCTAAATCATCTTCATTCATTATCAATTCAAACTTCCACCGCATTTCATACCAATCTGCAAAAACCCAAAACTTTTCACACCCAAATCTGCGTTAATTATAGGCAAATTGTATGTTGTTCAACTTCTTCACCCTTCACTGAGAAGAATTCGGTGGAGCGATACCAAAGGGAGCACTGGGTTTATCCTGTTATATCTCAATTTCAATCATCTCAATCTCAAGAACACTGTCAATCGTCTTCGCCGCCGCAGCCGTCTAAGGATAGAAGGGAAGATGACATTGCTTCCCAACTACCAGAACTCAAGAAGCTGCTTGAGGTATTGAGAGAAAAGAGAGAGAGCCAATCACAAGGTGGCGGCAATTCTGGTGTTATTGGAAATGTGTATTTGGTGGGCACTGGTCCAGGGGACCCTGAGTTACTCACTGTAAAAGCTCTCAAAATAATTCAATCTGCTGATCTTCTATTGTACGATCGCCTCGTCTCGAATGATGTGTTGGAAATGGTGGGCCCCAATGCTAAGCTTCTGTATGTTGGGAAGACTGCTGGCTACCATAGTAGGACACAGGAGGAGATTCATCAGCTGCTGTTGAGTTTTGCTGAAGCTGGTGCTAATGTTGTTAGACTTAAAGGCGGAGATCCTCTTATTTTCGGACGCGGTGGCGAAGAGATGGATTTCCTGCAGCAGAAGGGGATTCAAGTTAAAGTTGTTCCAGGTATTACTGCTGCTTCAGGTATATCAGCTGAACTAGGAATTCCGTTGACTCATCGAGGCATCGCAACTAGTGTTAGATATCTCACTGGACACTCAAGGAATGGGGGAACTGATCCGTTGTATGTAGCAGAGAATGCTGCTGACCCTGACTGTACCCTAGTTGTATATATGGGTTTGGCAACACTTCCTGCTCTTGCCCAGAAATTGGTGCAACATGGCCTGCCTAATGATACTCCTGCTGTTGCAGTGGAAAGAGGGACAACTGCTCAACAGCGTTTGAAAGGAGCCAAGGCAAAGCGTTTGCAGACGGAGCAACACTCAGCAGATCTTCCCACATTTTCTTCCTGGTTTCAACTGTATGCAGGCATAAATTGCACCAAACAAAGAAAGCAAAATGTTCCCTTGATTGTCATAGAGCTTACATTGAGTAAACTGCTAATGGTATTTTCAAAGTTGGAGAATCTTGCTGATAAAATTAGATGTAGTGGACTGGTATCCCCTACACTGATCATCATCGGGAAGGTTGTTGCACTTTCCCCATTGTGGATGCAATCCTGTGAAGAACCAGCTGAGCTTGTGGATGCAAAGTAG

Protein sequence

MASLNHLHSLSIQTSTAFHTNLQKPKTFHTQICVNYRQIVCCSTSSPFTEKNSVERYQREHWVYPVISQFQSSQSQEHCQSSSPPQPSKDRREDDIASQLPELKKLLEVLREKRESQSQGGGNSGVIGNVYLVGTGPGDPELLTVKALKIIQSADLLLYDRLVSNDVLEMVGPNAKLLYVGKTAGYHSRTQEEIHQLLLSFAEAGANVVRLKGGDPLIFGRGGEEMDFLQQKGIQVKVVPGITAASGISAELGIPLTHRGIATSVRYLTGHSRNGGTDPLYVAENAADPDCTLVVYMGLATLPALAQKLVQHGLPNDTPAVAVERGTTAQQRLKGAKAKRLQTEQHSADLPTFSSWFQLYAGINCTKQRKQNVPLIVIELTLSKLLMVFSKLENLADKIRCSGLVSPTLIIIGKVVALSPLWMQSCEEPAELVDAK

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo00478.1	Spo00478.1	mRNA

Homology

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BLAST of Spo00478.1 vs. NCBI nr
Match: gi|902207872|gb|KNA15795.1| (hypothetical protein SOVF_094870 [Spinacia oleracea])

HSP 1 Score: 656.4 bits (1692), Expect = 3.600e-185
Identity = 335/341 (98.24%), Postives = 337/341 (98.83%), Query Frame = 1


		  
Query: 1   MASLNHLHSLSIQTSTAFHTNLQKPKTFHTQICVNYRQIVCCSTSSPFTEKNSVERYQRE 60
           MASLNHLHSLSIQTSTAFHTNLQKPKTFHTQICVNYRQIVCCSTSSPFTEKNSVERYQRE
Sbjct: 1   MASLNHLHSLSIQTSTAFHTNLQKPKTFHTQICVNYRQIVCCSTSSPFTEKNSVERYQRE 60

Query: 61  HWVYPVISQFQSSQSQEHCQSSSPPQPSKDRREDDIASQLPELKKLLEVLREKRESQSQG 120
           HWVYPVISQFQSSQSQEHCQSSSPPQPSKDRREDDIASQLPELKKLLEVLREKRESQSQG
Sbjct: 61  HWVYPVISQFQSSQSQEHCQSSSPPQPSKDRREDDIASQLPELKKLLEVLREKRESQSQG 120

Query: 121 GGNSGVIGNVYLVGTGPGDPELLTVKALKIIQSADLLLYDRLVSNDVLEMVGPNAKLLYV 180
           GGNSGVIGNVYLVGTGPGDPELLTVKALKIIQSADLLLYDRLVSNDVLEMVGPNAKLLYV
Sbjct: 121 GGNSGVIGNVYLVGTGPGDPELLTVKALKIIQSADLLLYDRLVSNDVLEMVGPNAKLLYV 180

Query: 181 GKTAGYHSRTQEEIHQLLLSFAEAGANVVRLKGGDPLIFGRGGEEMDFLQQKGIQVKVVP 240
           GKTAGYHSRTQEEIHQLLLSFAEAGANVVRLKGGDPLIFGRGGEEMDFLQQKGIQVKVVP
Sbjct: 181 GKTAGYHSRTQEEIHQLLLSFAEAGANVVRLKGGDPLIFGRGGEEMDFLQQKGIQVKVVP 240

Query: 241 GITAASGISAELGIPLTHRGIATSVRYLTGHSRNGGTDPLYVAENAADPDCTLVVYMGLA 300
           GITAASGISAELGIPLTHRGIATSVRYLTGHSRNGGTDPLYVAENAADPDCTLVVYMGLA
Sbjct: 241 GITAASGISAELGIPLTHRGIATSVRYLTGHSRNGGTDPLYVAENAADPDCTLVVYMGLA 300

Query: 301 TLPALAQKLVQHGLPNDTPAVAVERGTTAQQRLKGAKAKRL 342
           TLPALAQKLVQHGLPNDTPAVAVERGTTAQQRL  +K + L
Sbjct: 301 TLPALAQKLVQHGLPNDTPAVAVERGTTAQQRLVFSKLENL 341

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BLAST of Spo00478.1 vs. NCBI nr
Match: gi|731376080|ref|XP_010667452.1| (PREDICTED: uroporphyrinogen-III C-methyltransferase [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 510.4 bits (1313), Expect = 3.200e-141
Identity = 274/348 (78.74%), Postives = 299/348 (85.92%), Query Frame = 1


		  
Query: 1   MASLNHLHSLSIQTSTAFHTNLQKPKTFHTQICVNYRQIVCCSTSSPFTEKNSVERYQRE 60
           MASLN L SLS Q  T F T+LQKPK FHTQ  ++Y++I C S SSPFTEK+SVERYQRE
Sbjct: 1   MASLNQLQSLSSQNPTTFLTHLQKPKFFHTQNSIHYKKITCLS-SSPFTEKHSVERYQRE 60

Query: 61  HWVYPVI----SQFQSSQSQEHCQSSSPPQPS---KDRREDDIASQLPELKKLLEVLREK 120
           HW+YPV     SQ  SS  +    SSS    S     RRE+DIA QLPELKKLLEVL+EK
Sbjct: 61  HWIYPVSVPINSQLPSSNDESSSLSSSSSPSSLSNSSRRENDIALQLPELKKLLEVLKEK 120

Query: 121 RESQSQGGGNSGVIGNVYLVGTGPGDPELLTVKALKIIQSADLLLYDRLVSNDVLEMVGP 180
           RE QS+G   + V GNV+LVGTGPGDP+LLT+KALK+IQSADLLLYDRLVSN VL++VGP
Sbjct: 121 REKQSEGLTENSV-GNVFLVGTGPGDPDLLTLKALKVIQSADLLLYDRLVSNHVLDLVGP 180

Query: 181 NAKLLYVGKTAGYHSRTQEEIHQLLLSFAEAGANVVRLKGGDPLIFGRGGEEMDFLQQKG 240
           NA+LLYVGKTAGYHSRTQEEIHQLLLSFAEAGANVVRLKGGDPLIFGRGGEEMDFLQQKG
Sbjct: 181 NARLLYVGKTAGYHSRTQEEIHQLLLSFAEAGANVVRLKGGDPLIFGRGGEEMDFLQQKG 240

Query: 241 IQVKVVPGITAASGISAELGIPLTHRGIATSVRYLTGHSRNGGTDPLYVAENAADPDCTL 300
           IQVKVVPGITAASGI AELGIPLTHRGIATSVRYLTGHSRNGGTDPL+VAENAADPDCTL
Sbjct: 241 IQVKVVPGITAASGIGAELGIPLTHRGIATSVRYLTGHSRNGGTDPLFVAENAADPDCTL 300

Query: 301 VVYMGLATLPALAQKLVQHGLPNDTPAVAVERGTTAQQRLKGAKAKRL 342
           VVYMGLATLP+LAQKL+QHGLP +TPAVAVERGTTAQQR+  AK + L
Sbjct: 301 VVYMGLATLPSLAQKLMQHGLPTETPAVAVERGTTAQQRMVFAKLEDL 346

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BLAST of Spo00478.1 vs. NCBI nr
Match: gi|970003229|ref|XP_015062390.1| (PREDICTED: siroheme synthase [Solanum pennellii])

HSP 1 Score: 438.3 bits (1126), Expect = 1.500e-119
Identity = 242/358 (67.60%), Postives = 284/358 (79.33%), Query Frame = 1


		  
Query: 1   MASLNHLHSLSIQTSTAFHTNLQKPKTF--HTQICVNYRQIVCCSTSSPFTEKNSVERYQ 60
           MA ++ + S+S   S++  T L K K+F  +   C+NY      S+SSPFTEK+S+ERYQ
Sbjct: 1   MALVSRIPSIS---SSSGATQLTKIKSFCVNPVCCINYAS---SSSSSPFTEKHSIERYQ 60

Query: 61  REHWVYPVISQFQSSQSQEHCQSSSP--PQPSKDRREDDIASQLPELKKLLEVLREKRES 120
           R+ W+Y            E   SS P  P PS  R + DIA QLPELKK+L+VLREKRE 
Sbjct: 61  RDSWIY--------KNELEQQSSSCPILPDPSYVR-DYDIALQLPELKKMLQVLREKREG 120

Query: 121 QSQGGGNSGVIGNVYLVGTGPGDPELLTVKALKIIQSADLLLYDRLVSNDVLEMVGPNAK 180
           +  GG +    GNV+LVGTGPGDPELLTVKAL++IQ+ADLLLYDRLVSN+VL++VGP+A+
Sbjct: 121 E--GGCDRKGPGNVFLVGTGPGDPELLTVKALRVIQNADLLLYDRLVSNEVLDLVGPDAR 180

Query: 181 LLYVGKTAGYHSRTQEEIHQLLLSFAEAGANVVRLKGGDPLIFGRGGEEMDFLQQKGIQV 240
           LLYVGKTAGYHSRTQEEIH+LLLSFAEAGANVVRLKGGDPL+FGRGGEEMDFLQQKGIQV
Sbjct: 181 LLYVGKTAGYHSRTQEEIHELLLSFAEAGANVVRLKGGDPLVFGRGGEEMDFLQQKGIQV 240

Query: 241 KVVPGITAASGISAELGIPLTHRGIATSVRYLTGHSRNGGTDPLYVAENAADPDCTLVVY 300
           KV+PGITAASGISAELGIPLTHRG+A SVR+LTGHSR GGTDPL+VAENAADPD TLVVY
Sbjct: 241 KVIPGITAASGISAELGIPLTHRGVANSVRFLTGHSRKGGTDPLFVAENAADPDSTLVVY 300

Query: 301 MGLATLPALAQKLVQHGLPNDTPAVAVERGTTAQQRLKGAKAKRLQTE--QHSADLPT 353
           MGL+TLP+LA KL+ HGLP DTPAVA+ERGTT QQR+  A+ K L  +   H  + PT
Sbjct: 301 MGLSTLPSLASKLIHHGLPTDTPAVAIERGTTPQQRMVFAELKNLADDIVSHQLESPT 341

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BLAST of Spo00478.1 vs. NCBI nr
Match: gi|460367465|ref|XP_004229594.1| (PREDICTED: uroporphyrinogen-III C-methyltransferase [Solanum lycopersicum])

HSP 1 Score: 438.3 bits (1126), Expect = 1.500e-119
Identity = 242/358 (67.60%), Postives = 284/358 (79.33%), Query Frame = 1


		  
Query: 1   MASLNHLHSLSIQTSTAFHTNLQKPKTF--HTQICVNYRQIVCCSTSSPFTEKNSVERYQ 60
           MA ++ + S+S   S++  T L K K+F  +   C+NY      S+SSPFTEK+S+ERYQ
Sbjct: 1   MALVSRIPSIS---SSSGATQLTKIKSFCVNPVCCINYAS---SSSSSPFTEKHSIERYQ 60

Query: 61  REHWVYPVISQFQSSQSQEHCQSSSP--PQPSKDRREDDIASQLPELKKLLEVLREKRES 120
           R+ W+Y            E   SS P  P PS  R + DIA QLPELKK+L+VLREKRE 
Sbjct: 61  RDSWIY--------KNELEQQSSSCPILPDPSYVR-DYDIALQLPELKKMLQVLREKREG 120

Query: 121 QSQGGGNSGVIGNVYLVGTGPGDPELLTVKALKIIQSADLLLYDRLVSNDVLEMVGPNAK 180
           +  GG +    GNV+LVGTGPGDPELLTVKAL++IQ+ADLLLYDRLVSN+VL++VGP+A+
Sbjct: 121 E--GGCDRKGPGNVFLVGTGPGDPELLTVKALRVIQNADLLLYDRLVSNEVLDLVGPDAR 180

Query: 181 LLYVGKTAGYHSRTQEEIHQLLLSFAEAGANVVRLKGGDPLIFGRGGEEMDFLQQKGIQV 240
           LLYVGKTAGYHSRTQEEIH+LLLSFAEAGANVVRLKGGDPL+FGRGGEEMDFLQQKGIQV
Sbjct: 181 LLYVGKTAGYHSRTQEEIHELLLSFAEAGANVVRLKGGDPLVFGRGGEEMDFLQQKGIQV 240

Query: 241 KVVPGITAASGISAELGIPLTHRGIATSVRYLTGHSRNGGTDPLYVAENAADPDCTLVVY 300
           KV+PGITAASGISAELGIPLTHRG+A SVR+LTGHSR GGTDPL+VAENAADPD TLVVY
Sbjct: 241 KVIPGITAASGISAELGIPLTHRGVANSVRFLTGHSRKGGTDPLFVAENAADPDSTLVVY 300

Query: 301 MGLATLPALAQKLVQHGLPNDTPAVAVERGTTAQQRLKGAKAKRLQTE--QHSADLPT 353
           MGL+TLP+LA KL+ HGLP DTPAVA+ERGTT QQR+  A+ K L  +   H  + PT
Sbjct: 301 MGLSTLPSLASKLIHHGLPTDTPAVAIERGTTPQQRMVFAELKNLADDIVSHQLESPT 341

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BLAST of Spo00478.1 vs. NCBI nr
Match: gi|697144117|ref|XP_009626179.1| (PREDICTED: uroporphyrinogen-III C-methyltransferase [Nicotiana tomentosiformis])

HSP 1 Score: 437.6 bits (1124), Expect = 2.600e-119
Identity = 238/354 (67.23%), Postives = 282/354 (79.66%), Query Frame = 1


		  
Query: 1   MASLNHLHSLSIQTSTAFHTNLQKPKTFHTQ--ICVNYRQIVCCSTSSPFTEKNSVERYQ 60
           MA ++ + S+S  +     T L+K K+F  +   C+NY       +SSPFTEK+S+ERYQ
Sbjct: 1   MALVSRIPSISPSSGA---TQLRKIKSFGLKPVSCINY----VSQSSSPFTEKHSIERYQ 60

Query: 61  REHWVYPVISQFQSSQSQEHCQSSSP--PQPSKDRREDDIASQLPELKKLLEVLREKRES 120
           R++W+Y            E    S P  P PS  R + DIA QLPELKK+L VL+EKR+S
Sbjct: 61  RDNWIY--------KNELEEKSGSCPILPDPSYVR-DYDIALQLPELKKMLRVLKEKRKS 120

Query: 121 QSQGGGNSGVIGNVYLVGTGPGDPELLTVKALKIIQSADLLLYDRLVSNDVLEMVGPNAK 180
           +  GGG+    GNVYLVGTGPGDPELLT+KAL++IQ+ADLLLYDRLVSN+VL++VGP+A+
Sbjct: 121 E--GGGDKRGPGNVYLVGTGPGDPELLTLKALRVIQNADLLLYDRLVSNEVLDLVGPDAR 180

Query: 181 LLYVGKTAGYHSRTQEEIHQLLLSFAEAGANVVRLKGGDPLIFGRGGEEMDFLQQKGIQV 240
           LLYVGKTAGYHSRTQEEIH+LLLSFAEAGANVVRLKGGDPL+FGRGGEEMDFLQQ+GIQV
Sbjct: 181 LLYVGKTAGYHSRTQEEIHELLLSFAEAGANVVRLKGGDPLVFGRGGEEMDFLQQQGIQV 240

Query: 241 KVVPGITAASGISAELGIPLTHRGIATSVRYLTGHSRNGGTDPLYVAENAADPDCTLVVY 300
           KV+PGITAASGISAELGIPLTHRG+A SVR+LTGHSR GGTDPL+VAENAADPDCTLVVY
Sbjct: 241 KVIPGITAASGISAELGIPLTHRGVANSVRFLTGHSRKGGTDPLFVAENAADPDCTLVVY 300

Query: 301 MGLATLPALAQKLVQHGLPNDTPAVAVERGTTAQQRLKGAKAKRLQTEQHSADL 351
           MGL+TLP+LA KL+ HGLP DTPAVA+ERGTT QQR+  A+ K L  +  S  L
Sbjct: 301 MGLSTLPSLASKLIHHGLPTDTPAVAIERGTTPQQRMVFAELKNLADDIASNQL 336

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BLAST of Spo00478.1 vs. UniProtKB/TrEMBL
Match: A0A0K9R8H8_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_094870 PE=3 SV=1)

HSP 1 Score: 656.4 bits (1692), Expect = 2.500e-185
Identity = 335/341 (98.24%), Postives = 337/341 (98.83%), Query Frame = 1


		  
Query: 1   MASLNHLHSLSIQTSTAFHTNLQKPKTFHTQICVNYRQIVCCSTSSPFTEKNSVERYQRE 60
           MASLNHLHSLSIQTSTAFHTNLQKPKTFHTQICVNYRQIVCCSTSSPFTEKNSVERYQRE
Sbjct: 1   MASLNHLHSLSIQTSTAFHTNLQKPKTFHTQICVNYRQIVCCSTSSPFTEKNSVERYQRE 60

Query: 61  HWVYPVISQFQSSQSQEHCQSSSPPQPSKDRREDDIASQLPELKKLLEVLREKRESQSQG 120
           HWVYPVISQFQSSQSQEHCQSSSPPQPSKDRREDDIASQLPELKKLLEVLREKRESQSQG
Sbjct: 61  HWVYPVISQFQSSQSQEHCQSSSPPQPSKDRREDDIASQLPELKKLLEVLREKRESQSQG 120

Query: 121 GGNSGVIGNVYLVGTGPGDPELLTVKALKIIQSADLLLYDRLVSNDVLEMVGPNAKLLYV 180
           GGNSGVIGNVYLVGTGPGDPELLTVKALKIIQSADLLLYDRLVSNDVLEMVGPNAKLLYV
Sbjct: 121 GGNSGVIGNVYLVGTGPGDPELLTVKALKIIQSADLLLYDRLVSNDVLEMVGPNAKLLYV 180

Query: 181 GKTAGYHSRTQEEIHQLLLSFAEAGANVVRLKGGDPLIFGRGGEEMDFLQQKGIQVKVVP 240
           GKTAGYHSRTQEEIHQLLLSFAEAGANVVRLKGGDPLIFGRGGEEMDFLQQKGIQVKVVP
Sbjct: 181 GKTAGYHSRTQEEIHQLLLSFAEAGANVVRLKGGDPLIFGRGGEEMDFLQQKGIQVKVVP 240

Query: 241 GITAASGISAELGIPLTHRGIATSVRYLTGHSRNGGTDPLYVAENAADPDCTLVVYMGLA 300
           GITAASGISAELGIPLTHRGIATSVRYLTGHSRNGGTDPLYVAENAADPDCTLVVYMGLA
Sbjct: 241 GITAASGISAELGIPLTHRGIATSVRYLTGHSRNGGTDPLYVAENAADPDCTLVVYMGLA 300

Query: 301 TLPALAQKLVQHGLPNDTPAVAVERGTTAQQRLKGAKAKRL 342
           TLPALAQKLVQHGLPNDTPAVAVERGTTAQQRL  +K + L
Sbjct: 301 TLPALAQKLVQHGLPNDTPAVAVERGTTAQQRLVFSKLENL 341

[Close]

BLAST of Spo00478.1 vs. UniProtKB/TrEMBL
Match: A0A0J8B653_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_008870 PE=3 SV=1)

HSP 1 Score: 510.4 bits (1313), Expect = 2.200e-141
Identity = 274/348 (78.74%), Postives = 299/348 (85.92%), Query Frame = 1


		  
Query: 1   MASLNHLHSLSIQTSTAFHTNLQKPKTFHTQICVNYRQIVCCSTSSPFTEKNSVERYQRE 60
           MASLN L SLS Q  T F T+LQKPK FHTQ  ++Y++I C S SSPFTEK+SVERYQRE
Sbjct: 1   MASLNQLQSLSSQNPTTFLTHLQKPKFFHTQNSIHYKKITCLS-SSPFTEKHSVERYQRE 60

Query: 61  HWVYPVI----SQFQSSQSQEHCQSSSPPQPS---KDRREDDIASQLPELKKLLEVLREK 120
           HW+YPV     SQ  SS  +    SSS    S     RRE+DIA QLPELKKLLEVL+EK
Sbjct: 61  HWIYPVSVPINSQLPSSNDESSSLSSSSSPSSLSNSSRRENDIALQLPELKKLLEVLKEK 120

Query: 121 RESQSQGGGNSGVIGNVYLVGTGPGDPELLTVKALKIIQSADLLLYDRLVSNDVLEMVGP 180
           RE QS+G   + V GNV+LVGTGPGDP+LLT+KALK+IQSADLLLYDRLVSN VL++VGP
Sbjct: 121 REKQSEGLTENSV-GNVFLVGTGPGDPDLLTLKALKVIQSADLLLYDRLVSNHVLDLVGP 180

Query: 181 NAKLLYVGKTAGYHSRTQEEIHQLLLSFAEAGANVVRLKGGDPLIFGRGGEEMDFLQQKG 240
           NA+LLYVGKTAGYHSRTQEEIHQLLLSFAEAGANVVRLKGGDPLIFGRGGEEMDFLQQKG
Sbjct: 181 NARLLYVGKTAGYHSRTQEEIHQLLLSFAEAGANVVRLKGGDPLIFGRGGEEMDFLQQKG 240

Query: 241 IQVKVVPGITAASGISAELGIPLTHRGIATSVRYLTGHSRNGGTDPLYVAENAADPDCTL 300
           IQVKVVPGITAASGI AELGIPLTHRGIATSVRYLTGHSRNGGTDPL+VAENAADPDCTL
Sbjct: 241 IQVKVVPGITAASGIGAELGIPLTHRGIATSVRYLTGHSRNGGTDPLFVAENAADPDCTL 300

Query: 301 VVYMGLATLPALAQKLVQHGLPNDTPAVAVERGTTAQQRLKGAKAKRL 342
           VVYMGLATLP+LAQKL+QHGLP +TPAVAVERGTTAQQR+  AK + L
Sbjct: 301 VVYMGLATLPSLAQKLMQHGLPTETPAVAVERGTTAQQRMVFAKLEDL 346

[Close]

BLAST of Spo00478.1 vs. UniProtKB/TrEMBL
Match: K4AY58_SOLLC (Uncharacterized protein OS=Solanum lycopersicum PE=3 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 1.100e-119
Identity = 242/358 (67.60%), Postives = 284/358 (79.33%), Query Frame = 1


		  
Query: 1   MASLNHLHSLSIQTSTAFHTNLQKPKTF--HTQICVNYRQIVCCSTSSPFTEKNSVERYQ 60
           MA ++ + S+S   S++  T L K K+F  +   C+NY      S+SSPFTEK+S+ERYQ
Sbjct: 1   MALVSRIPSIS---SSSGATQLTKIKSFCVNPVCCINYAS---SSSSSPFTEKHSIERYQ 60

Query: 61  REHWVYPVISQFQSSQSQEHCQSSSP--PQPSKDRREDDIASQLPELKKLLEVLREKRES 120
           R+ W+Y            E   SS P  P PS  R + DIA QLPELKK+L+VLREKRE 
Sbjct: 61  RDSWIY--------KNELEQQSSSCPILPDPSYVR-DYDIALQLPELKKMLQVLREKREG 120

Query: 121 QSQGGGNSGVIGNVYLVGTGPGDPELLTVKALKIIQSADLLLYDRLVSNDVLEMVGPNAK 180
           +  GG +    GNV+LVGTGPGDPELLTVKAL++IQ+ADLLLYDRLVSN+VL++VGP+A+
Sbjct: 121 E--GGCDRKGPGNVFLVGTGPGDPELLTVKALRVIQNADLLLYDRLVSNEVLDLVGPDAR 180

Query: 181 LLYVGKTAGYHSRTQEEIHQLLLSFAEAGANVVRLKGGDPLIFGRGGEEMDFLQQKGIQV 240
           LLYVGKTAGYHSRTQEEIH+LLLSFAEAGANVVRLKGGDPL+FGRGGEEMDFLQQKGIQV
Sbjct: 181 LLYVGKTAGYHSRTQEEIHELLLSFAEAGANVVRLKGGDPLVFGRGGEEMDFLQQKGIQV 240

Query: 241 KVVPGITAASGISAELGIPLTHRGIATSVRYLTGHSRNGGTDPLYVAENAADPDCTLVVY 300
           KV+PGITAASGISAELGIPLTHRG+A SVR+LTGHSR GGTDPL+VAENAADPD TLVVY
Sbjct: 241 KVIPGITAASGISAELGIPLTHRGVANSVRFLTGHSRKGGTDPLFVAENAADPDSTLVVY 300

Query: 301 MGLATLPALAQKLVQHGLPNDTPAVAVERGTTAQQRLKGAKAKRLQTE--QHSADLPT 353
           MGL+TLP+LA KL+ HGLP DTPAVA+ERGTT QQR+  A+ K L  +   H  + PT
Sbjct: 301 MGLSTLPSLASKLIHHGLPTDTPAVAIERGTTPQQRMVFAELKNLADDIVSHQLESPT 341

[Close]

BLAST of Spo00478.1 vs. UniProtKB/TrEMBL
Match: W8SGE9_TOBAC (Siroheme synthase OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 2.400e-119
Identity = 236/345 (68.41%), Postives = 279/345 (80.87%), Query Frame = 1


		  
Query: 1   MASLNHLHSLSIQTSTAFHTNLQKPKTFHTQ--ICVNYRQIVCCSTSSPFTEKNSVERYQ 60
           MA ++ + S+S  +     T L+K K+F  +   C+NY       +SSPFTEK+S+ERYQ
Sbjct: 1   MALVSRIPSISPSSGA---TQLRKIKSFGLKPVSCINY----VSQSSSPFTEKHSIERYQ 60

Query: 61  REHWVYPVISQFQSSQSQEHCQSSSP--PQPSKDRREDDIASQLPELKKLLEVLREKRES 120
           R++W+Y            E    S P  P PS  R + DIA QLPELKK+L VL+EKR+S
Sbjct: 61  RDNWIY--------KNELEEKSGSCPILPDPSYVR-DYDIALQLPELKKMLRVLKEKRKS 120

Query: 121 QSQGGGNSGVIGNVYLVGTGPGDPELLTVKALKIIQSADLLLYDRLVSNDVLEMVGPNAK 180
           +  GGG+    GNVYLVGTGPGDPELLT+KAL++IQ+ADLLLYDRLVSN+VL++VGP+A+
Sbjct: 121 E--GGGDKRGPGNVYLVGTGPGDPELLTLKALRVIQNADLLLYDRLVSNEVLDLVGPDAR 180

Query: 181 LLYVGKTAGYHSRTQEEIHQLLLSFAEAGANVVRLKGGDPLIFGRGGEEMDFLQQKGIQV 240
           LLYVGKTAGYHSRTQEEIH+LLLSFAEAGANVVRLKGGDPL+FGRGGEEMDFLQQ+GIQV
Sbjct: 181 LLYVGKTAGYHSRTQEEIHELLLSFAEAGANVVRLKGGDPLVFGRGGEEMDFLQQQGIQV 240

Query: 241 KVVPGITAASGISAELGIPLTHRGIATSVRYLTGHSRNGGTDPLYVAENAADPDCTLVVY 300
           KV+PGITAASGISAELGIPLTHRG+A SVR+LTGHSR GGTDPL+VAENAADPDCTLVVY
Sbjct: 241 KVIPGITAASGISAELGIPLTHRGVANSVRFLTGHSRKGGTDPLFVAENAADPDCTLVVY 300

Query: 301 MGLATLPALAQKLVQHGLPNDTPAVAVERGTTAQQRLKGAKAKRL 342
           MGL+TLP+LA KL+ HGLP DTPAVA+ERGTT QQR+  A+ K L
Sbjct: 301 MGLSTLPSLASKLIHHGLPTDTPAVAIERGTTPQQRMVFAELKNL 327

[Close]

BLAST of Spo00478.1 vs. UniProtKB/TrEMBL
Match: M4E9T5_BRARP (Uncharacterized protein OS=Brassica rapa subsp. pekinensis PE=3 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 2.400e-119
Identity = 238/350 (68.00%), Postives = 279/350 (79.71%), Query Frame = 1


		  
Query: 12  IQTSTAFHTNLQKPK----TFHTQIC-VNYRQIVCCSTSSPFTEKNSVERYQREHWVYPV 71
           +Q   +F +NL+  K    T H  +  ++Y+  +  S+SSPFTEK+SVERYQR+ W+Y  
Sbjct: 4   VQRIPSFSSNLRNWKSTNSTNHKPVSSLHYKTQITASSSSPFTEKHSVERYQRDQWLYKA 63

Query: 72  ISQFQSSQSQEHCQSSSPPQPSKDR------REDDIASQLPELKKLLEVLREKRESQSQG 131
            +            S SPP PS ++      RE+DIASQLPELKKLLEVLREKRES  +G
Sbjct: 64  STP-----------SPSPPPPSPNQQDEVFVRENDIASQLPELKKLLEVLREKRESGCRG 123

Query: 132 GGNSGVIGNVYLVGTGPGDPELLTVKALKIIQSADLLLYDRLVSNDVLEMVGPNAKLLYV 191
           G      G+V+LVGTGPGDPELLT+KA+++IQSADLLLYDRLVSNDVLE+V P+A+LLYV
Sbjct: 124 GDCGP--GDVFLVGTGPGDPELLTLKAVRVIQSADLLLYDRLVSNDVLELVAPDARLLYV 183

Query: 192 GKTAGYHSRTQEEIHQLLLSFAEAGANVVRLKGGDPLIFGRGGEEMDFLQQKGIQVKVVP 251
           GKTAGYHSRTQEEIH+LLLSFAEAGA VVRLKGGDPL+FGRGGEEMDFLQQ+GI+V+V+P
Sbjct: 184 GKTAGYHSRTQEEIHELLLSFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIRVQVIP 243

Query: 252 GITAASGISAELGIPLTHRGIATSVRYLTGHSRNGGTDPLYVAENAADPDCTLVVYMGLA 311
           GITAASGI+AELGIPLTHRG+ATSVR+LTGHSR GGTDPL+VAENAADPD TLVVYMGL 
Sbjct: 244 GITAASGIAAELGIPLTHRGVATSVRFLTGHSRKGGTDPLFVAENAADPDTTLVVYMGLG 303

Query: 312 TLPALAQKLVQHGLPNDTPAVAVERGTTAQQRLKGAKAKRLQTEQHSADL 351
           TLP+LAQKL+ HGLP DTPAVAVERGTT  QR   A+ K   TE  +A L
Sbjct: 304 TLPSLAQKLMDHGLPCDTPAVAVERGTTPLQRNVFAELKDFATEIQAAGL 340

[Close]

BLAST of Spo00478.1 vs. ExPASy Swiss-Prot
Match: CYSG_PSEA7 (Siroheme synthase OS=Pseudomonas aeruginosa (strain PA7) GN=cysG PE=3 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 2.400e-56
Identity = 120/240 (50.00%), Postives = 157/240 (65.42%), Query Frame = 1


		  
Query: 115 ESQSQGGGNSGVIGNVYLVGTGPGDPELLTVKALKIIQSADLLLYDRLVSNDVLEMVGPN 174
           E +  GG      G VYLVG GPGDP+LLT +AL+++Q AD++LYDRLV+  +LE+   +
Sbjct: 204 EERLAGGLAHMATGEVYLVGAGPGDPDLLTFRALRLMQQADVVLYDRLVAPAILELCRRD 263

Query: 175 AKLLYVGKTAGYHSRTQEEIHQLLLSFAEAGANVVRLKGGDPLIFGRGGEEMDFLQQKGI 234
           A+ LYVGK    H+  QE I++LL+  A  G  V+RLKGGDP IFGRGGEE+D L   GI
Sbjct: 264 AERLYVGKRRAEHAVPQERINRLLVELASQGKRVLRLKGGDPFIFGRGGEEIDELAAHGI 323

Query: 235 QVKVVPGITAASGISAELGIPLTHRGIATSVRYLTGHSRNGGTDPLYVAENAADPDCTLV 294
             +VVPGITAASG +A  GIPLTHR  A SVR++TGH ++G TD  +  ++   P  TLV
Sbjct: 324 PFQVVPGITAASGCAAYAGIPLTHRDHAQSVRFVTGHLKDGTTDLPW--KDLVAPGQTLV 383

Query: 295 VYMGLATLPALAQKLVQHGLPNDTPAVAVERGTTAQQRLKGAKAKRLQ--TEQHSADLPT 353
            YMGL  LP + ++LV HG    TPA  +++GTTAQQR+     + L     +H    PT
Sbjct: 384 FYMGLVGLPVICEQLVAHGRSAQTPAALIQQGTTAQQRVFSGTLENLPQLVAEHEVHAPT 441

[Close]

BLAST of Spo00478.1 vs. ExPASy Swiss-Prot
Match: CYSG_PSEAB (Siroheme synthase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=cysG PE=3 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 2.400e-56
Identity = 119/240 (49.58%), Postives = 158/240 (65.83%), Query Frame = 1


		  
Query: 115 ESQSQGGGNSGVIGNVYLVGTGPGDPELLTVKALKIIQSADLLLYDRLVSNDVLEMVGPN 174
           E +  GG      G VYLVG GPGDP+LLT +AL+++Q AD++LYDRLV+  +LE+   +
Sbjct: 204 EERLAGGLAHIATGEVYLVGAGPGDPDLLTFRALRLMQQADVVLYDRLVAPSILELCRRD 263

Query: 175 AKLLYVGKTAGYHSRTQEEIHQLLLSFAEAGANVVRLKGGDPLIFGRGGEEMDFLQQKGI 234
           A+ LYVGK    H+  Q+ I++LL+  A  G  V+RLKGGDP IFGRGGEE+D L  +GI
Sbjct: 264 AERLYVGKRRAEHAVPQDRINRLLVELASQGKRVLRLKGGDPFIFGRGGEEIDELAARGI 323

Query: 235 QVKVVPGITAASGISAELGIPLTHRGIATSVRYLTGHSRNGGTDPLYVAENAADPDCTLV 294
             +VVPGITAASG +A  GIPLTHR  A SVR++TGH ++G TD  +  ++   P  TLV
Sbjct: 324 PFQVVPGITAASGCAAYAGIPLTHRDHAQSVRFVTGHLKDGTTDLPW--QDLVAPGQTLV 383

Query: 295 VYMGLATLPALAQKLVQHGLPNDTPAVAVERGTTAQQRLKGAKAKRLQ--TEQHSADLPT 353
            YMGL  LP + ++LV HG    TPA  +++GTTAQQR+     + L     +H    PT
Sbjct: 384 FYMGLVGLPVICEQLVAHGRSAQTPAALIQQGTTAQQRVFTGTLENLPQLVAEHEVHAPT 441

[Close]

BLAST of Spo00478.1 vs. ExPASy Swiss-Prot
Match: CYSG_PSEAE (Siroheme synthase OS=Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) GN=cysG PE=3 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 7.000e-56
Identity = 119/240 (49.58%), Postives = 156/240 (65.00%), Query Frame = 1


		  
Query: 115 ESQSQGGGNSGVIGNVYLVGTGPGDPELLTVKALKIIQSADLLLYDRLVSNDVLEMVGPN 174
           E    GG      G VYLVG GPGDP+LLT +AL+++Q AD++LYDRLV+  +LE+   +
Sbjct: 204 EEHLAGGLAHIATGEVYLVGAGPGDPDLLTFRALRLMQQADVVLYDRLVAPSILELCRRD 263

Query: 175 AKLLYVGKTAGYHSRTQEEIHQLLLSFAEAGANVVRLKGGDPLIFGRGGEEMDFLQQKGI 234
           A+ LYVGK    H+  Q+ I++LL+  A  G  V+RLKGGDP IFGRGGEE+D L   GI
Sbjct: 264 AERLYVGKRRAEHAVPQDRINRLLVELASQGKRVLRLKGGDPFIFGRGGEEIDELAAHGI 323

Query: 235 QVKVVPGITAASGISAELGIPLTHRGIATSVRYLTGHSRNGGTDPLYVAENAADPDCTLV 294
             +VVPGITAASG +A  GIPLTHR  A SVR++TGH ++G TD  +  ++   P  TLV
Sbjct: 324 PFQVVPGITAASGCAAYAGIPLTHRDHAQSVRFVTGHLKDGTTDLPW--QDLVAPGQTLV 383

Query: 295 VYMGLATLPALAQKLVQHGLPNDTPAVAVERGTTAQQRLKGAKAKRLQ--TEQHSADLPT 353
            YMGL  LP + ++LV HG    TPA  +++GTTAQQR+     + L     +H    PT
Sbjct: 384 FYMGLVGLPVICEQLVAHGRSAQTPAALIQQGTTAQQRVFTGTLENLPQLVAEHEVHAPT 441

[Close]

BLAST of Spo00478.1 vs. ExPASy Swiss-Prot
Match: CYSG_PSEA8 (Siroheme synthase OS=Pseudomonas aeruginosa (strain LESB58) GN=cysG PE=3 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 7.000e-56
Identity = 119/240 (49.58%), Postives = 156/240 (65.00%), Query Frame = 1


		  
Query: 115 ESQSQGGGNSGVIGNVYLVGTGPGDPELLTVKALKIIQSADLLLYDRLVSNDVLEMVGPN 174
           E    GG      G VYLVG GPGDP+LLT +AL+++Q AD++LYDRLV+  +LE+   +
Sbjct: 204 EEHLAGGLAHIATGEVYLVGAGPGDPDLLTFRALRLMQQADVVLYDRLVAPSILELCRRD 263

Query: 175 AKLLYVGKTAGYHSRTQEEIHQLLLSFAEAGANVVRLKGGDPLIFGRGGEEMDFLQQKGI 234
           A+ LYVGK    H+  Q+ I++LL+  A  G  V+RLKGGDP IFGRGGEE+D L   GI
Sbjct: 264 AERLYVGKRRAEHAVPQDRINRLLVELASQGKRVLRLKGGDPFIFGRGGEEIDELAAHGI 323

Query: 235 QVKVVPGITAASGISAELGIPLTHRGIATSVRYLTGHSRNGGTDPLYVAENAADPDCTLV 294
             +VVPGITAASG +A  GIPLTHR  A SVR++TGH ++G TD  +  ++   P  TLV
Sbjct: 324 PFQVVPGITAASGCAAYAGIPLTHRDHAQSVRFVTGHLKDGTTDLPW--QDLVAPGQTLV 383

Query: 295 VYMGLATLPALAQKLVQHGLPNDTPAVAVERGTTAQQRLKGAKAKRLQ--TEQHSADLPT 353
            YMGL  LP + ++LV HG    TPA  +++GTTAQQR+     + L     +H    PT
Sbjct: 384 FYMGLVGLPVICEQLVAHGRSAQTPAALIQQGTTAQQRVFTGTLENLPQLVAEHEVHAPT 441

[Close]

BLAST of Spo00478.1 vs. ExPASy Swiss-Prot
Match: CYSG_PSEU2 (Siroheme synthase OS=Pseudomonas syringae pv. syringae (strain B728a) GN=cysG PE=3 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 7.000e-56
Identity = 118/227 (51.98%), Postives = 155/227 (68.28%), Query Frame = 1


		  
Query: 128 GNVYLVGTGPGDPELLTVKALKIIQSADLLLYDRLVSNDVLEMVGPNAKLLYVGKTAGYH 187
           G VYLVG GPGDP+LLT +AL+++Q AD++LYDRLV+  +L++   +A+ +YVGK    H
Sbjct: 216 GEVYLVGAGPGDPDLLTFRALRLMQQADVVLYDRLVAPTILDLCRRDAERVYVGKRRAEH 275

Query: 188 SRTQEEIHQLLLSFAEAGANVVRLKGGDPLIFGRGGEEMDFLQQKGIQVKVVPGITAASG 247
           +  QE+I+Q L++ A+ G  VVRLKGGDP IFGRGGEE++ L   GI  +VVPGITAASG
Sbjct: 276 AVPQEQINQQLVALAKQGKRVVRLKGGDPFIFGRGGEEIEELAAHGIPFQVVPGITAASG 335

Query: 248 ISAELGIPLTHRGIATSVRYLTGHSRNGGTDPLYVAENAADPDCTLVVYMGLATLPALAQ 307
            +A  GIPLTHR  A SVR++TGH +NG TD  +   +   P  TLV YMGL  LP + +
Sbjct: 336 CAAYAGIPLTHRDHAQSVRFITGHLKNGTTDLPW--SDLVAPAQTLVFYMGLIGLPVICE 395

Query: 308 KLVQHGLPNDTPAVAVERGTTAQQRL-KGAKAKRLQ-TEQHSADLPT 353
           +L++HG   DTPA  VE+GTT  QR+  G  A   Q   +H    PT
Sbjct: 396 QLIRHGRSADTPAALVEQGTTVNQRVFTGTLANLPQLVAEHDVHAPT 440

[Close]

BLAST of Spo00478.1 vs. TAIR (Arabidopsis)
Match: AT5G40850.1 (urophorphyrin methylase 1)

HSP 1 Score: 430.6 bits (1106), Expect = 1.100e-120
Identity = 235/343 (68.51%), Postives = 273/343 (79.59%), Query Frame = 1


		  
Query: 12  IQTSTAFHTNLQKPKTFHTQICVNYRQIVCCSTSSPFTEKNSVERYQREHWVYPVISQFQ 71
           I  S++   N Q+ +T  T IC  +      S+SSPFTEK+SVERYQR+ W+Y  +    
Sbjct: 7   IPISSSSIRNWQQARTNLTPICCLHYN-TASSSSSPFTEKHSVERYQRDQWLYKAV---- 66

Query: 72  SSQSQEHCQSSSP-PQPSKDR---REDDIASQLPELKKLLEVLREKRESQSQGGGNSGVI 131
                E    S+P P P +D    RE+DIASQLPELKKLL VL+EKR    +GG      
Sbjct: 67  -----EPTPPSTPSPSPFEDEVFVRENDIASQLPELKKLLAVLKEKRVKGCKGGDCGP-- 126

Query: 132 GNVYLVGTGPGDPELLTVKALKIIQSADLLLYDRLVSNDVLEMVGPNAKLLYVGKTAGYH 191
           G+VYLVGTGPGDPELLT+KA+++IQSADLLLYDRLVSNDVLE+V P+A+LLYVGKTAGYH
Sbjct: 127 GDVYLVGTGPGDPELLTLKAVRVIQSADLLLYDRLVSNDVLELVAPDARLLYVGKTAGYH 186

Query: 192 SRTQEEIHQLLLSFAEAGANVVRLKGGDPLIFGRGGEEMDFLQQKGIQVKVVPGITAASG 251
           SRTQEEIH+LLL+FAEAGA VVRLKGGDPL+FGRGGEEMDFLQQ+GI+V+V+PGITAASG
Sbjct: 187 SRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIRVQVIPGITAASG 246

Query: 252 ISAELGIPLTHRGIATSVRYLTGHSRNGGTDPLYVAENAADPDCTLVVYMGLATLPALAQ 311
           I+AELGIPLTHRG+ATSVR+LTGHSR GGTDPL+VAENAADPD TLVVYMGL TLP+LAQ
Sbjct: 247 IAAELGIPLTHRGVATSVRFLTGHSRKGGTDPLFVAENAADPDTTLVVYMGLGTLPSLAQ 306

Query: 312 KLVQHGLPNDTPAVAVERGTTAQQRLKGAKAKRLQTEQHSADL 351
           KL+ HGLP+DTPAVAVERGTT  QR   A+ K   TE  SA L
Sbjct: 307 KLMDHGLPSDTPAVAVERGTTPLQRTVFAELKDFATEIQSAGL 337

The following BLAST results are available for this feature:

BLAST of Spo00478.1 vs. NCBI nr
BLAST of Spo00478.1 vs. UniProtKB/TrEMBL
BLAST of Spo00478.1 vs. ExPASy Swiss-Prot
BLAST of Spo00478.1 vs. TAIR (Arabidopsis)

BLAST of Spo00478.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902207872\|gb\|KNA15795.1\|	3.6e-185	98.2	hypothetical protein SOVF_0948...	[more]
gi\|731376080\|ref\|XP_010667452.1\|	3.2e-141	78.7	PREDICTED: uroporphyrinogen-II...	[more]
gi\|970003229\|ref\|XP_015062390.1\|	1.5e-119	67.6	PREDICTED: siroheme synthase [...	[more]
gi\|460367465\|ref\|XP_004229594.1\|	1.5e-119	67.6	PREDICTED: uroporphyrinogen-II...	[more]
gi\|697144117\|ref\|XP_009626179.1\|	2.6e-119	67.2	PREDICTED: uroporphyrinogen-II...	[more]

BLAST of Spo00478.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9R8H8_SPIOL	2.5e-185	98.2	Uncharacterized protein OS=Spi...	[more]
A0A0J8B653_BETVU	2.2e-141	78.7	Uncharacterized protein OS=Bet...	[more]
K4AY58_SOLLC	1.1e-119	67.6	Uncharacterized protein OS=Sol...	[more]
W8SGE9_TOBAC	2.4e-119	68.4	Siroheme synthase OS=Nicotiana...	[more]
M4E9T5_BRARP	2.4e-119	68.0	Uncharacterized protein OS=Bra...	[more]

BLAST of Spo00478.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
CYSG_PSEA7	2.4e-56	50.0	Siroheme synthase OS=Pseudomon...	[more]
CYSG_PSEAB	2.4e-56	49.5	Siroheme synthase OS=Pseudomon...	[more]
CYSG_PSEAE	7.0e-56	49.5	Siroheme synthase OS=Pseudomon...	[more]
CYSG_PSEA8	7.0e-56	49.5	Siroheme synthase OS=Pseudomon...	[more]
CYSG_PSEU2	7.0e-56	51.9	Siroheme synthase OS=Pseudomon...	[more]

BLAST of Spo00478.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1


Match Name	E-value	Identity	Description
AT5G40850.1	1.1e-120	68.5	urophorphyrin methylase 1	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000878	Tetrapyrrole methylase	PFAM	PF00590	TP_methylase	coord: 130..337 score: 7.2
IPR000878	Tetrapyrrole methylase	unknown	SSF53790	Tetrapyrrole methylase	coord: 127..346 score: 2.36
IPR003043	Uroporphiryn-III C-methyltransferase, conserved site	PROSITE	PS00839	SUMT_1	coord: 133..147 scor
IPR003043	Uroporphiryn-III C-methyltransferase, conserved site	PROSITE	PS00840	SUMT_2	coord: 208..241 scor
IPR006366	Uroporphyrin-III C-methyltransferase	TIGRFAMs	TIGR01469	TIGR01469	coord: 128..346 score: 2.5
IPR014776	Tetrapyrrole methylase, subdomain 2	GENE3D	3.30.950.10		coord: 244..333 score: 8.1E-29coord: 368..421 score: 1.
IPR014777	Tetrapyrrole methylase, subdomain 1	GENE3D	3.40.1010.10		coord: 128..243 score: 3.4
None	No IPR available	PANTHER	PTHR21091	METHYLTETRAHYDROFOLATE:HOMOCYSTEINE METHYLTRANSFERASE RELATED	coord: 77..350 score: 1.4
None	No IPR available	PANTHER	PTHR21091:SF16	SIROHEME SYNTHASE	coord: 77..350 score: 1.4

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015994	chlorophyll metabolic process
biological_process	GO:0032259	methylation
biological_process	GO:0006779	porphyrin-containing compound biosynthetic process
biological_process	GO:0008152	metabolic process
biological_process	GO:0010207	photosystem II assembly
biological_process	GO:0016556	mRNA modification
biological_process	GO:0019354	siroheme biosynthetic process
biological_process	GO:0006567	threonine catabolic process
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0009535	chloroplast thylakoid membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0009523	photosystem II
molecular_function	GO:0043115	precorrin-2 dehydrogenase activity
molecular_function	GO:0004851	uroporphyrin-III C-methyltransferase activity
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0008168	methyltransferase activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo14350	0.66	Barchart \| Table
Spo03592	0.66	Barchart \| Table
Spo08407	0.66	Barchart \| Table
Spo00214	0.66	Barchart \| Table
Spo10835	0.65	Barchart \| Table
Spo00943	0.65	Barchart \| Table
Spo09434	0.65	Barchart \| Table
Spo06953	0.65	Barchart \| Table