Spo00496 (gene) | SpinachBase

Overview


Name	Spo00496
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	hydroxyproline-rich glycoprotein family protein
Location	SpoScf_00043 : 415999 .. 416373 (-)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTTCGTCAAAGTGTATGTTGATGTTCTGTCTACTTATCACCATGTCAATGTCATGCATCGACGCAGCACGCCATCTGTTGCAGGCAGTGCCTGGGTTGCCCAATCCGACCACTGTCATGCCACCATTGCCCTCTATTCCGACAACTCTTCCTCAGCCAGCAGCTCTACCTAAGCTTAGTATTCCACCTATGTCAAGCACACAAATTCCGTCTTTGCCAAACATGCCCTCCATTCCAAACATGGCTCTCCCTCCCATGCCAAACCTACCAACCACCATTCCCACTTCCATTCCTAACTTTCCAACCTCCATTCCCAACTTTCCAACCTCTATCCCATCTATTCCTTTCCTTTCCCCACCTCCTTCAAACTAA

mRNA sequence

ATGGGTTCGTCAAAGTGTATGTTGATGTTCTGTCTACTTATCACCATGTCAATGTCATGCATCGACGCAGCACGCCATCTGTTGCAGGCAGTGCCTGGGTTGCCCAATCCGACCACTGTCATGCCACCATTGCCCTCTATTCCGACAACTCTTCCTCAGCCAGCAGCTCTACCTAAGCTTAGTATTCCACCTATGTCAAGCACACAAATTCCGTCTTTGCCAAACATGCCCTCCATTCCAAACATGGCTCTCCCTCCCATGCCAAACCTACCAACCACCATTCCCACTTCCATTCCTAACTTTCCAACCTCCATTCCCAACTTTCCAACCTCTATCCCATCTATTCCTTTCCTTTCCCCACCTCCTTCAAACTAA

Coding sequence (CDS)

ATGGGTTCGTCAAAGTGTATGTTGATGTTCTGTCTACTTATCACCATGTCAATGTCATGCATCGACGCAGCACGCCATCTGTTGCAGGCAGTGCCTGGGTTGCCCAATCCGACCACTGTCATGCCACCATTGCCCTCTATTCCGACAACTCTTCCTCAGCCAGCAGCTCTACCTAAGCTTAGTATTCCACCTATGTCAAGCACACAAATTCCGTCTTTGCCAAACATGCCCTCCATTCCAAACATGGCTCTCCCTCCCATGCCAAACCTACCAACCACCATTCCCACTTCCATTCCTAACTTTCCAACCTCCATTCCCAACTTTCCAACCTCTATCCCATCTATTCCTTTCCTTTCCCCACCTCCTTCAAACTAA

Protein sequence

MGSSKCMLMFCLLITMSMSCIDAARHLLQAVPGLPNPTTVMPPLPSIPTTLPQPAALPKLSIPPMSSTQIPSLPNMPSIPNMALPPMPNLPTTIPTSIPNFPTSIPNFPTSIPSIPFLSPPPSN

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo00496.1	Spo00496.1	mRNA

Homology

[Close]

BLAST of Spo00496.1 vs. NCBI nr
Match: gi|567867323|ref|XP_006426284.1| (hypothetical protein CICLE_v10027253mg [Citrus clementina])

HSP 1 Score: 85.1 bits (209), Expect = 9.300e-14
Identity = 65/130 (50.00%), Postives = 86/130 (66.15%), Query Frame = 1


		  
Query: 1   MGSSKCMLMFCLLITMSMSCID---AARHLLQAVPGLPNPTTVMPPLPSIPTTLPQPAAL 60
           M + K   +  ++I +S+S  +   AARHLLQA+P  P P   +PP+PSIPT LPQ A L
Sbjct: 1   MAALKHCFLLSIIIALSLSSFEMSMAARHLLQALP--PMPKVALPPMPSIPT-LPQ-ATL 60

Query: 61  PKLSIPPMSST--QIPSLPNMPSIPNMALPPMPNLPT-TIPTSIPNFPTSIPNFPTSIPS 120
           P L   P   T  ++P+LP MP+ P + LPP+P+ P  TIPT+ P    SIPN P++IP+
Sbjct: 61  PPLPTLPNQPTLPKLPTLPPMPAAPKVTLPPLPSNPLPTIPTTFP----SIPNIPSTIPT 120

Query: 121 IPFLSPPPSN 125
           IPFLSPPPSN
Sbjct: 121 IPFLSPPPSN 122

[Close]

BLAST of Spo00496.1 vs. NCBI nr
Match: gi|951044207|ref|XP_014518565.1| (PREDICTED: vegetative cell wall protein gp1-like [Vigna radiata var. radiata])

HSP 1 Score: 84.0 bits (206), Expect = 2.100e-13
Identity = 73/154 (47.40%), Postives = 93/154 (60.39%), Query Frame = 1


		  
Query: 1   MGSSKCMLMFCLLITMSMSCID---AARHLLQA----VPGLP---NPTTVMPPLPSIPTT 60
           M SS C + F   I MS S +D   AARHLLQ     +PG+P   NPT  +PPLPS PT 
Sbjct: 1   MASSSCFI-FAFFIAMSFSSMDIGLAARHLLQTTTPNLPGIPSFTNPT--LPPLPSFPT- 60

Query: 61  LPQPAALPKLSIPPMSSTQIPSLPNMPSIPNM------ALPPMPNLPTT---IPTSIPNF 120
           LPQ    P+ ++PP+ +   P+LP +PSIP +       LP +P++PTT   +PT+IPN 
Sbjct: 61  LPQ----PQATVPPLPTIPAPTLPTLPSIPALPQGNVPPLPTIPSIPTTLPPLPTNIPNI 120

Query: 121 PT----------SIPNF--PTSIPSIPFLSPPPS 124
           PT          SIP F  PT++PSIPFLSPPPS
Sbjct: 121 PTIPQLKVPPLPSIPTFTIPTTMPSIPFLSPPPS 146

[Close]

BLAST of Spo00496.1 vs. NCBI nr
Match: gi|643734128|gb|KDP40971.1| (hypothetical protein JCGZ_24970 [Jatropha curcas])

HSP 1 Score: 83.6 bits (205), Expect = 2.700e-13
Identity = 61/132 (46.21%), Postives = 78/132 (59.09%), Query Frame = 1


		  
Query: 1   MGSSKCMLMFCLLITMSMSCID---AARHLLQ-----AVPGLPNPTTVMPPLPSIPTTLP 60
           M S KC ++    I +S + +D   AARHLLQ     ++P +P PT   PPLPS   TLP
Sbjct: 1   MASFKCSILIAFFIALSFASMDVGLAARHLLQMPPLPSIPNMPKPT--FPPLPSNIPTLP 60

Query: 61  QPAALPKLSIPPMSSTQIPSLPNMPSIPNMALPPMPNLPTTIPTSIPNFPTSIPNFPTSI 120
           QP  LP L   P +   +PSLP MP++P + LPP+P++P            SIP  PT+I
Sbjct: 61  QPT-LPTL---PTTQPPLPSLPTMPTVPKVTLPPLPSMP------------SIPTIPTTI 114

Query: 121 PSIPFLSPPPSN 125
           PSIPFLSPPP N
Sbjct: 121 PSIPFLSPPPGN 114

[Close]

BLAST of Spo00496.1 vs. NCBI nr
Match: gi|802578018|ref|XP_012069305.1| (PREDICTED: proline-rich receptor-like protein kinase PERK2 [Jatropha curcas])

HSP 1 Score: 83.6 bits (205), Expect = 2.700e-13
Identity = 62/134 (46.27%), Postives = 80/134 (59.70%), Query Frame = 1


		  
Query: 1   MGSSKCMLMFCLLITMSMSCID---AARHLLQ-----AVPGLPNPTTVMPPLPSIPTTLP 60
           M S KC ++    I +S + +D   AARHLLQ     ++P +P PT   PPLPS   TLP
Sbjct: 1   MASFKCSILIAFFIALSFASMDVGLAARHLLQMPPLPSIPNMPKPT--FPPLPSNIPTLP 60

Query: 61  QPAALPKLSIPPMSSTQIPSLPNMPSIPNMALPPMPNLPTTIPTSIPNFPT--SIPNFPT 120
           QP  LP L   P +   +PS P MP++P   LP +P +PT    ++P  P+  SIP  PT
Sbjct: 61  QPT-LPTL---PTTQPPLPSWPTMPTVPKPPLPSLPTMPTVPKVTLPPLPSMPSIPTIPT 120

Query: 121 SIPSIPFLSPPPSN 125
           +IPSIPFLSPPP N
Sbjct: 121 TIPSIPFLSPPPGN 128

[Close]

BLAST of Spo00496.1 vs. NCBI nr
Match: gi|224140731|ref|XP_002323732.1| (hypothetical protein POPTR_0017s07310g [Populus trichocarpa])

HSP 1 Score: 82.8 bits (203), Expect = 4.600e-13
Identity = 66/135 (48.89%), Postives = 84/135 (62.22%), Query Frame = 1


		  
Query: 1   MGSSKCMLMFCLLITMSMSCIDAARHLLQ-----AVPGLPNPTTVMPPLPSIPTTLPQPA 60
           M S  C ++  L I +S+S  +AAR LLQ     AVP LP PT  +PP+PSIPT LPQP 
Sbjct: 1   MASFSCFIL-ALFIALSISGGEAARQLLQLPPLPAVPNLPKPT--LPPMPSIPT-LPQPT 60

Query: 61  ------ALPKLSIPPMSSTQIPSLPNMPSIPNMALPPMPNLPTTIPTSIPNFPTSIPNFP 120
                 +LPK ++PP+     PSLP MPS+P + LPP+P++P     S+P  P      P
Sbjct: 61  LPTAQPSLPKPTLPPL-----PSLPTMPSLPKVTLPPLPSMP-----SMPTIP-----IP 116

Query: 121 TSIPSIPFLSPPPSN 125
           T+IPSIPFLSPPP N
Sbjct: 121 TTIPSIPFLSPPPGN 116

[Close]

BLAST of Spo00496.1 vs. UniProtKB/TrEMBL
Match: V4UJJ2_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10027253mg PE=4 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 6.500e-14
Identity = 65/130 (50.00%), Postives = 86/130 (66.15%), Query Frame = 1


		  
Query: 1   MGSSKCMLMFCLLITMSMSCID---AARHLLQAVPGLPNPTTVMPPLPSIPTTLPQPAAL 60
           M + K   +  ++I +S+S  +   AARHLLQA+P  P P   +PP+PSIPT LPQ A L
Sbjct: 1   MAALKHCFLLSIIIALSLSSFEMSMAARHLLQALP--PMPKVALPPMPSIPT-LPQ-ATL 60

Query: 61  PKLSIPPMSST--QIPSLPNMPSIPNMALPPMPNLPT-TIPTSIPNFPTSIPNFPTSIPS 120
           P L   P   T  ++P+LP MP+ P + LPP+P+ P  TIPT+ P    SIPN P++IP+
Sbjct: 61  PPLPTLPNQPTLPKLPTLPPMPAAPKVTLPPLPSNPLPTIPTTFP----SIPNIPSTIPT 120

Query: 121 IPFLSPPPSN 125
           IPFLSPPPSN
Sbjct: 121 IPFLSPPPSN 122

[Close]

BLAST of Spo00496.1 vs. UniProtKB/TrEMBL
Match: A0A067EZN3_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g047312mg PE=4 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 6.500e-14
Identity = 65/130 (50.00%), Postives = 86/130 (66.15%), Query Frame = 1


		  
Query: 1   MGSSKCMLMFCLLITMSMSCID---AARHLLQAVPGLPNPTTVMPPLPSIPTTLPQPAAL 60
           M + K   +  ++I +S+S  +   AARHLLQA+P  P P   +PP+PSIPT LPQ A L
Sbjct: 1   MAALKHCFLLSIIIALSLSSFEMSMAARHLLQALP--PMPKVALPPMPSIPT-LPQ-ATL 60

Query: 61  PKLSIPPMSST--QIPSLPNMPSIPNMALPPMPNLPT-TIPTSIPNFPTSIPNFPTSIPS 120
           P L   P   T  ++P+LP MP+ P + LPP+P+ P  TIPT+ P    SIPN P++IP+
Sbjct: 61  PPLPTLPNQPTLPKLPTLPPMPAAPKVTLPPLPSNPLPTIPTTFP----SIPNIPSTIPT 120

Query: 121 IPFLSPPPSN 125
           IPFLSPPPSN
Sbjct: 121 IPFLSPPPSN 122

[Close]

BLAST of Spo00496.1 vs. UniProtKB/TrEMBL
Match: A0A067KXM6_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_24970 PE=4 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 1.900e-13
Identity = 61/132 (46.21%), Postives = 78/132 (59.09%), Query Frame = 1


		  
Query: 1   MGSSKCMLMFCLLITMSMSCID---AARHLLQ-----AVPGLPNPTTVMPPLPSIPTTLP 60
           M S KC ++    I +S + +D   AARHLLQ     ++P +P PT   PPLPS   TLP
Sbjct: 1   MASFKCSILIAFFIALSFASMDVGLAARHLLQMPPLPSIPNMPKPT--FPPLPSNIPTLP 60

Query: 61  QPAALPKLSIPPMSSTQIPSLPNMPSIPNMALPPMPNLPTTIPTSIPNFPTSIPNFPTSI 120
           QP  LP L   P +   +PSLP MP++P + LPP+P++P            SIP  PT+I
Sbjct: 61  QPT-LPTL---PTTQPPLPSLPTMPTVPKVTLPPLPSMP------------SIPTIPTTI 114

Query: 121 PSIPFLSPPPSN 125
           PSIPFLSPPP N
Sbjct: 121 PSIPFLSPPPGN 114

[Close]

BLAST of Spo00496.1 vs. UniProtKB/TrEMBL
Match: B9IJ95_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0017s07310g PE=4 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 3.200e-13
Identity = 66/135 (48.89%), Postives = 84/135 (62.22%), Query Frame = 1


		  
Query: 1   MGSSKCMLMFCLLITMSMSCIDAARHLLQ-----AVPGLPNPTTVMPPLPSIPTTLPQPA 60
           M S  C ++  L I +S+S  +AAR LLQ     AVP LP PT  +PP+PSIPT LPQP 
Sbjct: 1   MASFSCFIL-ALFIALSISGGEAARQLLQLPPLPAVPNLPKPT--LPPMPSIPT-LPQPT 60

Query: 61  ------ALPKLSIPPMSSTQIPSLPNMPSIPNMALPPMPNLPTTIPTSIPNFPTSIPNFP 120
                 +LPK ++PP+     PSLP MPS+P + LPP+P++P     S+P  P      P
Sbjct: 61  LPTAQPSLPKPTLPPL-----PSLPTMPSLPKVTLPPLPSMP-----SMPTIP-----IP 116

Query: 121 TSIPSIPFLSPPPSN 125
           T+IPSIPFLSPPP N
Sbjct: 121 TTIPSIPFLSPPPGN 116

[Close]

BLAST of Spo00496.1 vs. UniProtKB/TrEMBL
Match: J3MTU3_ORYBR (Uncharacterized protein OS=Oryza brachyantha PE=4 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 5.500e-13
Identity = 61/141 (43.26%), Postives = 87/141 (61.70%), Query Frame = 1


		  
Query: 1   MGSSKCMLMFCLLITMSMSCIDAARHLLQAVPGLPNPTTVMPPLPSIPTTLP-QPA--AL 60
           M +S C L+  LL   +++C  AARHLL      P     +P +P++PTTLP  P+  A+
Sbjct: 1   MAASGCHLLALLLAFTAINCSYAARHLLDTAAAAPEAAPALPSIPTVPTTLPPMPSIPAV 60

Query: 61  PKLSIPPMSSTQIPSLP------NMPSIPNM--ALPPMPNLPTTIPTSIPNFPTSIPNFP 120
           PKL++PP+ S  +PSLP       +P+IP +   LPPMP++PTTIP+     PT+IP+ P
Sbjct: 61  PKLTVPPIPS--VPSLPIPAIPTTVPTIPTVPVTLPPMPSIPTTIPSIPTTIPTTIPSIP 120

Query: 121 TSIPS--------IPFLSPPP 123
           T+IP+        IPF+SPPP
Sbjct: 121 TTIPTIPGFQMPPIPFMSPPP 139

[Close]

BLAST of Spo00496.1 vs. TAIR (Arabidopsis)
Match: AT2G22510.1 (hydroxyproline-rich glycoprotein family protein)

HSP 1 Score: 70.9 bits (172), Expect = 6.400e-13
Identity = 59/131 (45.04%), Postives = 72/131 (54.96%), Query Frame = 1


		  
Query: 8   LMFCLLITMSMSCIDAARHLLQAVPGL---------PNPTTVMPPLPSIPTTLPQPAALP 67
           L+  L  T   + + A+RHLLQ+ P +         P PTT MPP P   T+LPQP A P
Sbjct: 8   LVIALTFTSFFTGLSASRHLLQSTPAITPPVTTTFPPLPTTTMPPFPP-STSLPQPTAFP 67

Query: 68  KLSIPPMSSTQIPSLPNMPSIPNMALPPMP-NLPTTIPTSIPNFPTSIPN-----FPTSI 124
                P+ S+QIPSLPN         P  P N+P     +IPNFP SIPN      PTSI
Sbjct: 68  -----PLPSSQIPSLPN---------PAQPINIPNFPQINIPNFPISIPNNFPFNLPTSI 123

[Close]

BLAST of Spo00496.1 vs. TAIR (Arabidopsis)
Match: AT4G38080.1 (hydroxyproline-rich glycoprotein family protein)

HSP 1 Score: 63.2 bits (152), Expect = 1.300e-10
Identity = 58/131 (44.27%), Postives = 72/131 (54.96%), Query Frame = 1


		  
Query: 8   LMFCLLITMSMSCIDAARHLLQAVPGL--------PNPTTVMPPLPSIPT----TLPQPA 67
           L+  L  T   + + A RHLLQ+ P          P P T MPP+PS+PT    TLPQP 
Sbjct: 12  LVIALTFTSFFTSLSAHRHLLQSTPVTQPPALTFPPLPKTTMPPVPSLPTPGQQTLPQPQ 71

Query: 68  -ALPK-LSIPPMSSTQIPSLPNMPSIPNMALPPMPNLPTTIPTSIPNFPTSIPNFPTSIP 124
             LP+   +PPM STQIPSL      PN   P +PN+P        NFP+   NFP + P
Sbjct: 72  PTLPQPTGLPPMPSTQIPSL------PNQVQPTIPNIPQI------NFPS---NFPFNFP 127

The following BLAST results are available for this feature:

BLAST of Spo00496.1 vs. NCBI nr
BLAST of Spo00496.1 vs. UniProtKB/TrEMBL
BLAST of Spo00496.1 vs. ExPASy Swiss-Prot
BLAST of Spo00496.1 vs. TAIR (Arabidopsis)

BLAST of Spo00496.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|567867323\|ref\|XP_006426284.1\|	9.3e-14	50.0	hypothetical protein CICLE_v10...	[more]
gi\|951044207\|ref\|XP_014518565.1\|	2.1e-13	47.4	PREDICTED: vegetative cell wal...	[more]
gi\|643734128\|gb\|KDP40971.1\|	2.7e-13	46.2	hypothetical protein JCGZ_2497...	[more]
gi\|802578018\|ref\|XP_012069305.1\|	2.7e-13	46.2	PREDICTED: proline-rich recept...	[more]
gi\|224140731\|ref\|XP_002323732.1\|	4.6e-13	48.8	hypothetical protein POPTR_001...	[more]

BLAST of Spo00496.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
V4UJJ2_9ROSI	6.5e-14	50.0	Uncharacterized protein OS=Cit...	[more]
A0A067EZN3_CITSI	6.5e-14	50.0	Uncharacterized protein OS=Cit...	[more]
A0A067KXM6_JATCU	1.9e-13	46.2	Uncharacterized protein OS=Jat...	[more]
B9IJ95_POPTR	3.2e-13	48.8	Uncharacterized protein OS=Pop...	[more]
J3MTU3_ORYBR	5.5e-13	43.2	Uncharacterized protein OS=Ory...	[more]

BLAST of Spo00496.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Spo00496.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 2


Match Name	E-value	Identity	Description
AT2G22510.1	6.4e-13	45.0	hydroxyproline-rich glycoprote...	[more]
AT4G38080.1	1.3e-10	44.2	hydroxyproline-rich glycoprote...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR33088	FAMILY NOT NAMED	coord: 12..124 score: 1.7
None	No IPR available	PROFILE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..20 score:

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046486	glycerolipid metabolic process
biological_process	GO:0042967	obsolete acyl-carrier-protein biosynthetic process
molecular_function	GO:0004366	glycerol-3-phosphate O-acyltransferase activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo15589	0.85	Barchart \| Table
Spo24296	0.85	Barchart \| Table
Spo21916	0.75	Barchart \| Table
Spo02244	0.72	Barchart \| Table
Spo08107	0.71	Barchart \| Table
Spo20985	0.68	Barchart \| Table
Spo00119	0.67	Barchart \| Table
Spo01756	0.67	Barchart \| Table
Spo21915	0.66	Barchart \| Table
Spo19880	0.66	Barchart \| Table
Spo19280	0.66	Barchart \| Table
Spo20591	0.65	Barchart \| Table