Spo01586 (gene) | SpinachBase

Overview


Name	Spo01586
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Cysteine synthase (2.5.1.47)
Location	chr2 : 50185267 .. 50189219 (+)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAACTATCCACAACGCATCAATCCCAAAACCAATTCGTCACCTTTAACCGAAACCCTGTTCACGATCATAAATTCTTAACAGCAGTTAAGGTACATCCTGACATCCACAAAAGCAGCCGAAGTTAAGTGCAAAGTATCAAATCAACAGTTGAACACCACTCACTATTATAAATTATAATGACGACAAAAGAGTTAAAGACCATTTCCCTACACAACCCACATGCTCTGTAGAAATGTCACTACAATACACAAATTCCCCCTTTCCCCAAAATTGCATTGAAAATTTGAGCCACCCTGGCCTTCAAAACCTTCACTTTCCACCCACCAACCATATATATACTTCTATATAACAAACATTAACAGACTCATATAAAACACATTGTTATTGCTATTTGTTCTGCTCAAAATTGTAGATTTGTAGATTTGTAGTTGGTAAACTGAAGAAATCATGGCAACTGTGTCAAGCTGTTTGCTGAGGAGATCAAGAACAGCCTCACGGATCTTTAAAACATCACTCAGATGTTTCTCCACCACTTCTTCTTCTGCACAAACTGTTTCTGGTTCATCTCCCTTTCCTTTTACTGGGACTAATATTAAAACCAATGTTTCCCAAGTGAGTTTATTTTAATTTTATTTTCTTTGAAAAATAATTTCATTTTATTAAGTGAATTTATTTGATTTGATCTAATCATGGTTCTTGTTAACATTGTTGATGGATTAACCTAATAACCTGGTGGATGTTGCATGGTACAACCGATTGTACAATACAAACTGTCGTTAATTTAGATCAGTTTTAGCTCTTATCTCATGTTTTAGCAATAAGTTTTTTCAAAGAGATTGATTTAAGGAGAAAAGACAAGATAGATTGTATTATAAGGTGGAGATTTCTCAATAGTGATCCAAATCAGTTGGATACCTTCTGTTTTTAGATAAGGGCAGCAGCTAAAATTGAGCATTGTTGCCTCTTCAACCCAGGGGCCCCACCTAGAATGGATTCCACTAGTGGGGACCACATGTTTTGATAATTTTGTACAAGTAATAATTCCTGAACTCAAGTGGTCTATCTAAGTGTCTTGAATTTTCTCTGACAATGAACCTTTGCCTGATGTTTACAATTGAATAGCCAAATATTCCCTGCTTTACTAAATAAAAACTACAGTATGACGGCTATGTTGACGACTTGACATAACCACTTCCTTATACTTTGTTCCATTGGCAATAAAGTGTATGTATCCCGTTTTGTGTCTCATATTATGTTCGATTATCTCTACTTATGCCGCGTTATCCACGTTCAACAATAACATTAGATATGGTTATGTGTCAAAATCGGAAAGATGTGACGTAAATTGGGACATATGATTTGATTGATTTCAACCTGTTTAATGTTGAGTGTAATTTGGATAGCTGATAGCTATGTGCTGATGCAGCACAAAATTCCCCTTCAGATTCTGCAAGTTAACTAAGCATGTTCTGACAAATGTGATAGAAATGTCAGAAAATTAAAATCATTTGGTATTGATTTTGTTGTACATGATTTTTATTGCAGCTTATTGGGAGAACTCCCCTAGTTTATCTAAGCAAAATTAGTGAAGGATCTGGAGCTTACATAGCCGTCAAGCAAGAGATGATGCAGCCTACTGCTAGTGTTAAAGATAGGTATTTACATACCGTTAATAAACCAACAATAGGGCAGTAATTAAGCCAGTAATTAAGATTTAAGACTTGACATATATATGTGCTAATTAAATACTTCTTGGAATATCATCTGCTGTAGACCTGCATTGGCTATGATTGAAGATGCAGAGAAAAAGGGATTAATTTCTCCAGGAAAGGTAAGATAAAACAGTCAAGTTTTCTGCAACTGAACTTAATGCTAACTGTTATGCTGTGTTGATGATCAGACGGTGTTGATCGAGCCAACATCAGGAAACATGGGAATAAGCATGGCGTTCATGGCGGCTATGAAGGGATACAAAATGGTATTGACAATGCCATCATACACAAGCATGGAAAGAAGGGTAGTAATGAGGGCTTTTGGTGCTGATCTTATTCTCACTGACCCTGACAAAGGAATGGGGGGAACTGTCAAAAAGGCTAACCAGCTTTTAGACTCCACCCCTGATGGTTTCATGCTTCAACAATTTAATAATCCTGCTAATACTCAGGTCCTCTCTTCTCTTGCACTCTTCATAATAGACAAATTTGTCAATTTAATCACTTCTACGATTAATATTGTCAGAGATAGGGATAATTAGTTGACTAATAATGGCTGAAAAAAACAGGTTCATTTTGAGACGACAGGTCCTGAAATATGGGAGGATACTCAAGGGAAAGTGGACATCTTTGTCATGGGAATCGGAAGTGGAGGCACTGTTTCTGGTGTTGGACGCTATCTCAAATCTCAAAATCCTAATGTGAAGGTATCATCTATATATATATATATATATATTTATAAGTGATGCTGCTTTGAATTGTGCTAGAGTAGTTTAATTACATATATGCCTAATGTGATTCATTTTTACTGATGTTATAGATCTATGGAGTTGAACCAGCTGAAAGCAACATACTCAATGGAGGCAAACCAGGTATTTCATGCGAAAACTTAAGTTAACAGCTCCTTAGTTAGTTGAACAAATAATCCGGCTTCTTGGCTTCTTGCTAATCTACTTTTTCTGTTAATGTAAATAGGTCCTCACCTTATAACTGGAAACGGGGTTGGGTTTAAACCAGAGATTTTAGACATGGATGTAATGGATGCAGTTCTCGAGGTAAAATTTCTGTTTTCTTTTCTTCTGTTTTTCCAACAATATCAAAGCTTAGTTCTCTATGTGCGAGTTTTCTGAACACGTACTTCATCACAGGTTAAAAGTGACGATGCTGTTAAGATGGCCAGGCAGTTGGCATTACAGGAAGGACTTTTGGTAAGAACAAATTATATGCTGGTCAGTGGCGGAGCCAGAATTTTTTTTTCGGGGTGTCGGTAGTGGCGTATCCAGGATTTTTAGTGTGTTGTGTCTGATAATCTTCCACAAAACACAATATATTACTAAAAAAAAAAGTATAGTCTTTTCTGATCCCCCTTGCACCTACCAGGCTCCGCCACTGATGCTGGTTTACTACACTTCTGAATTTCTGATCATATCTGCATCACAAACTTTCAGAACTAGCTATAATCTTAACAGAGATCTGAACATCTTTAGTATGGCTAAAATTATCTGATTTTCACTTTGTTGAATATGTAGGTTGGGATATCATCAGGGGCTAATACAATTGCTGCCCTTGATCTTGCTAAGAGGCCAGAAAACAAGGGCAAACTAATTGTGGTATGAAATAGCTGTCCTATTTATATATGAAGTTATGAACTACTACTACTAACTCTGTTTGGATATAATTGTCACGTCGATAGTTCAATTATGCTATTAAAGTGGCAATTAATTCCAGATAGAGGCAGTGCTGTTTTATAATTGTAGAGTTGTAGTACTAATAAGGATTATGTTTGTTTCTTGATCAAAGACGATTCACCCGAGTTTTGGAGAGCGATATCTGTCGTCTGCCTTGTTCAAGGAGCTGAGGGAAGAGGCTGAAAACATGCAACCAGTCCCTGTTGAATGATTCCAGCATCTTATAGCTCCAGATATGCTAAATAAAGGATCTGTTACTGTTTTGTCACTACTACTAGTATATGACAGTAACTACATGATATCTCTTTGAGAAAAATTGTAAAATTGCAAGTCAATTGTGTATTAATTTTCAATGAAATTGTTAGGGATGAAAATTATTTATCAATGAAGTAATTGTAATTCTTTTATGATGCAGCATTAATAGTATCCTTAATTCACCATTTTATAGTACTCTGTATAATGATTAGTATGGAGTAGCTCTTAGGCTATGTTCTCCTCAACTTAAACCATGTTCTTTAGAGCAGAGCTGAACTGAACCGAACTGAATGCT

mRNA sequence

AAAAACTATCCACAACGCATCAATCCCAAAACCAATTCGTCACCTTTAACCGAAACCCTGTTCACGATCATAAATTCTTAACAGCAGTTAAGGTACATCCTGACATCCACAAAAGCAGCCGAAGTTAAGTGCAAAGTATCAAATCAACAGTTGAACACCACTCACTATTATAAATTATAATGACGACAAAAGAGTTAAAGACCATTTCCCTACACAACCCACATGCTCTGTAGAAATGTCACTACAATACACAAATTCCCCCTTTCCCCAAAATTGCATTGAAAATTTGAGCCACCCTGGCCTTCAAAACCTTCACTTTCCACCCACCAACCATATATATACTTCTATATAACAAACATTAACAGACTCATATAAAACACATTGTTATTGCTATTTGTTCTGCTCAAAATTGTAGATTTGTAGATTTGTAGTTGGTAAACTGAAGAAATCATGGCAACTGTGTCAAGCTGTTTGCTGAGGAGATCAAGAACAGCCTCACGGATCTTTAAAACATCACTCAGATGTTTCTCCACCACTTCTTCTTCTGCACAAACTGTTTCTGGTTCATCTCCCTTTCCTTTTACTGGGACTAATATTAAAACCAATGTTTCCCAACTTATTGGGAGAACTCCCCTAGTTTATCTAAGCAAAATTAGTGAAGGATCTGGAGCTTACATAGCCGTCAAGCAAGAGATGATGCAGCCTACTGCTAGTGTTAAAGATAGACCTGCATTGGCTATGATTGAAGATGCAGAGAAAAAGGGATTAATTTCTCCAGGAAAGACGGTGTTGATCGAGCCAACATCAGGAAACATGGGAATAAGCATGGCGTTCATGGCGGCTATGAAGGGATACAAAATGGTATTGACAATGCCATCATACACAAGCATGGAAAGAAGGGTAGTAATGAGGGCTTTTGGTGCTGATCTTATTCTCACTGACCCTGACAAAGGAATGGGGGGAACTGTCAAAAAGGCTAACCAGCTTTTAGACTCCACCCCTGATGGTTTCATGCTTCAACAATTTAATAATCCTGCTAATACTCAGGTTCATTTTGAGACGACAGGTCCTGAAATATGGGAGGATACTCAAGGGAAAGTGGACATCTTTGTCATGGGAATCGGAAGTGGAGGCACTGTTTCTGGTGTTGGACGCTATCTCAAATCTCAAAATCCTAATGTGAAGATCTATGGAGTTGAACCAGCTGAAAGCAACATACTCAATGGAGGCAAACCAGGTCCTCACCTTATAACTGGAAACGGGGTTGGGTTTAAACCAGAGATTTTAGACATGGATGTAATGGATGCAGTTCTCGAGGTTAAAAGTGACGATGCTGTTAAGATGGCCAGGCAGTTGGCATTACAGGAAGGACTTTTGGTTGGGATATCATCAGGGGCTAATACAATTGCTGCCCTTGATCTTGCTAAGAGGCCAGAAAACAAGGGCAAACTAATTGTGACGATTCACCCGAGTTTTGGAGAGCGATATCTGTCGTCTGCCTTGTTCAAGGAGCTGAGGGAAGAGGCTGAAAACATGCAACCAGTCCCTGTTGAATGATTCCAGCATCTTATAGCTCCAGATATGCTAAATAAAGGATCTGTTACTGTTTTGTCACTACTACTAGTATATGACAGTAACTACATGATATCTCTTTGAGAAAAATTGTAAAATTGCAAGTCAATTGTGTATTAATTTTCAATGAAATTGTTAGGGATGAAAATTATTTATCAATGAAGTAATTGTAATTCTTTTATGATGCAGCATTAATAGTATCCTTAATTCACCATTTTATAGTACTCTGTATAATGATTAGTATGGAGTAGCTCTTAGGCTATGTTCTCCTCAACTTAAACCATGTTCTTTAGAGCAGAGCTGAACTGAACCGAACTGAATGCT

Coding sequence (CDS)

ATGGCAACTGTGTCAAGCTGTTTGCTGAGGAGATCAAGAACAGCCTCACGGATCTTTAAAACATCACTCAGATGTTTCTCCACCACTTCTTCTTCTGCACAAACTGTTTCTGGTTCATCTCCCTTTCCTTTTACTGGGACTAATATTAAAACCAATGTTTCCCAACTTATTGGGAGAACTCCCCTAGTTTATCTAAGCAAAATTAGTGAAGGATCTGGAGCTTACATAGCCGTCAAGCAAGAGATGATGCAGCCTACTGCTAGTGTTAAAGATAGACCTGCATTGGCTATGATTGAAGATGCAGAGAAAAAGGGATTAATTTCTCCAGGAAAGACGGTGTTGATCGAGCCAACATCAGGAAACATGGGAATAAGCATGGCGTTCATGGCGGCTATGAAGGGATACAAAATGGTATTGACAATGCCATCATACACAAGCATGGAAAGAAGGGTAGTAATGAGGGCTTTTGGTGCTGATCTTATTCTCACTGACCCTGACAAAGGAATGGGGGGAACTGTCAAAAAGGCTAACCAGCTTTTAGACTCCACCCCTGATGGTTTCATGCTTCAACAATTTAATAATCCTGCTAATACTCAGGTTCATTTTGAGACGACAGGTCCTGAAATATGGGAGGATACTCAAGGGAAAGTGGACATCTTTGTCATGGGAATCGGAAGTGGAGGCACTGTTTCTGGTGTTGGACGCTATCTCAAATCTCAAAATCCTAATGTGAAGATCTATGGAGTTGAACCAGCTGAAAGCAACATACTCAATGGAGGCAAACCAGGTCCTCACCTTATAACTGGAAACGGGGTTGGGTTTAAACCAGAGATTTTAGACATGGATGTAATGGATGCAGTTCTCGAGGTTAAAAGTGACGATGCTGTTAAGATGGCCAGGCAGTTGGCATTACAGGAAGGACTTTTGGTTGGGATATCATCAGGGGCTAATACAATTGCTGCCCTTGATCTTGCTAAGAGGCCAGAAAACAAGGGCAAACTAATTGTGACGATTCACCCGAGTTTTGGAGAGCGATATCTGTCGTCTGCCTTGTTCAAGGAGCTGAGGGAAGAGGCTGAAAACATGCAACCAGTCCCTGTTGAATGA

Protein sequence

MATVSSCLLRRSRTASRIFKTSLRCFSTTSSSAQTVSGSSPFPFTGTNIKTNVSQLIGRTPLVYLSKISEGSGAYIAVKQEMMQPTASVKDRPALAMIEDAEKKGLISPGKTVLIEPTSGNMGISMAFMAAMKGYKMVLTMPSYTSMERRVVMRAFGADLILTDPDKGMGGTVKKANQLLDSTPDGFMLQQFNNPANTQVHFETTGPEIWEDTQGKVDIFVMGIGSGGTVSGVGRYLKSQNPNVKIYGVEPAESNILNGGKPGPHLITGNGVGFKPEILDMDVMDAVLEVKSDDAVKMARQLALQEGLLVGISSGANTIAALDLAKRPENKGKLIVTIHPSFGERYLSSALFKELREEAENMQPVPVE

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo01586.1	Spo01586.1	mRNA

Homology

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BLAST of Spo01586.1 vs. NCBI nr
Match: gi|75282478|sp|Q43153.1|CYSC_SPIOL (RecName: Full=Bifunctional L-3-cyanoalanine synthase/cysteine synthase, mitochondrial; AltName: Full=Beta-cyanoalanine synthase; Short=SoCAS; AltName: Full=Beta-substituted Ala synthase 3-1; Short=SPIol-Bsas3-1; AltName: Full=Cysteine synthase C; Flags: Precursor)

HSP 1 Score: 711.8 bits (1836), Expect = 6.000e-202
Identity = 368/368 (100.00%), Postives = 368/368 (100.00%), Query Frame = 1


		  
Query: 1   MATVSSCLLRRSRTASRIFKTSLRCFSTTSSSAQTVSGSSPFPFTGTNIKTNVSQLIGRT 60
           MATVSSCLLRRSRTASRIFKTSLRCFSTTSSSAQTVSGSSPFPFTGTNIKTNVSQLIGRT
Sbjct: 1   MATVSSCLLRRSRTASRIFKTSLRCFSTTSSSAQTVSGSSPFPFTGTNIKTNVSQLIGRT 60

Query: 61  PLVYLSKISEGSGAYIAVKQEMMQPTASVKDRPALAMIEDAEKKGLISPGKTVLIEPTSG 120
           PLVYLSKISEGSGAYIAVKQEMMQPTASVKDRPALAMIEDAEKKGLISPGKTVLIEPTSG
Sbjct: 61  PLVYLSKISEGSGAYIAVKQEMMQPTASVKDRPALAMIEDAEKKGLISPGKTVLIEPTSG 120

Query: 121 NMGISMAFMAAMKGYKMVLTMPSYTSMERRVVMRAFGADLILTDPDKGMGGTVKKANQLL 180
           NMGISMAFMAAMKGYKMVLTMPSYTSMERRVVMRAFGADLILTDPDKGMGGTVKKANQLL
Sbjct: 121 NMGISMAFMAAMKGYKMVLTMPSYTSMERRVVMRAFGADLILTDPDKGMGGTVKKANQLL 180

Query: 181 DSTPDGFMLQQFNNPANTQVHFETTGPEIWEDTQGKVDIFVMGIGSGGTVSGVGRYLKSQ 240
           DSTPDGFMLQQFNNPANTQVHFETTGPEIWEDTQGKVDIFVMGIGSGGTVSGVGRYLKSQ
Sbjct: 181 DSTPDGFMLQQFNNPANTQVHFETTGPEIWEDTQGKVDIFVMGIGSGGTVSGVGRYLKSQ 240

Query: 241 NPNVKIYGVEPAESNILNGGKPGPHLITGNGVGFKPEILDMDVMDAVLEVKSDDAVKMAR 300
           NPNVKIYGVEPAESNILNGGKPGPHLITGNGVGFKPEILDMDVMDAVLEVKSDDAVKMAR
Sbjct: 241 NPNVKIYGVEPAESNILNGGKPGPHLITGNGVGFKPEILDMDVMDAVLEVKSDDAVKMAR 300

Query: 301 QLALQEGLLVGISSGANTIAALDLAKRPENKGKLIVTIHPSFGERYLSSALFKELREEAE 360
           QLALQEGLLVGISSGANTIAALDLAKRPENKGKLIVTIHPSFGERYLSSALFKELREEAE
Sbjct: 301 QLALQEGLLVGISSGANTIAALDLAKRPENKGKLIVTIHPSFGERYLSSALFKELREEAE 360

Query: 361 NMQPVPVE 369
           NMQPVPVE
Sbjct: 361 NMQPVPVE 368

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BLAST of Spo01586.1 vs. NCBI nr
Match: gi|902197360|gb|KNA13398.1| (hypothetical protein SOVF_117360 [Spinacia oleracea])

HSP 1 Score: 699.9 bits (1805), Expect = 2.400e-198
Identity = 368/388 (94.85%), Postives = 368/388 (94.85%), Query Frame = 1


		  
Query: 1   MATVSSCLLRRSRTASRIFKTSLRCFSTTSSSAQTVSGSSPFPFTGTNIKTNVSQLIGRT 60
           MATVSSCLLRRSRTASRIFKTSLRCFSTTSSSAQTVSGSSPFPFTGTNIKTNVSQLIGRT
Sbjct: 1   MATVSSCLLRRSRTASRIFKTSLRCFSTTSSSAQTVSGSSPFPFTGTNIKTNVSQLIGRT 60

Query: 61  PLVYLSKISEGSGAYIAVKQEMMQPTASVKDRPALAMIEDAEKKGLISPGKTVLIEPTSG 120
           PLVYLSKISEGSGAYIAVKQEMMQPTASVKDRPALAMIEDAEKKGLISPGKTVLIEPTSG
Sbjct: 61  PLVYLSKISEGSGAYIAVKQEMMQPTASVKDRPALAMIEDAEKKGLISPGKTVLIEPTSG 120

Query: 121 NMGISMAFMAAMKGYKMVLTMPSYTSMERRVVMRAFGADLILTDPDKGMGGTVKKANQLL 180
           NMGISMAFMAAMKGYKMVLTMPSYTSMERRVVMRAFGADLILTDPDKGMGGTVKKANQLL
Sbjct: 121 NMGISMAFMAAMKGYKMVLTMPSYTSMERRVVMRAFGADLILTDPDKGMGGTVKKANQLL 180

Query: 181 DSTPDGFMLQQFNNPANTQVHFETTGPEIWEDTQGKVDIFVMGIGSGGTVSGVGRYLKSQ 240
           DSTPDGFMLQQFNNPANTQVHFETTGPEIWEDTQGKVDIFVMGIGSGGTVSGVGRYLKSQ
Sbjct: 181 DSTPDGFMLQQFNNPANTQVHFETTGPEIWEDTQGKVDIFVMGIGSGGTVSGVGRYLKSQ 240

Query: 241 NPNVKIYGVEPAESNILNGGKPGPHLITGNGVGFKPEILDMDVMDAVLE----------- 300
           NPNVKIYGVEPAESNILNGGKPGPHLITGNGVGFKPEILDMDVMDAVLE           
Sbjct: 241 NPNVKIYGVEPAESNILNGGKPGPHLITGNGVGFKPEILDMDVMDAVLEVKSDDAVKMAR 300

Query: 301 ---------VKSDDAVKMARQLALQEGLLVGISSGANTIAALDLAKRPENKGKLIVTIHP 360
                    VKSDDAVKMARQLALQEGLLVGISSGANTIAALDLAKRPENKGKLIVTIHP
Sbjct: 301 QLALQEGLLVKSDDAVKMARQLALQEGLLVGISSGANTIAALDLAKRPENKGKLIVTIHP 360

Query: 361 SFGERYLSSALFKELREEAENMQPVPVE 369
           SFGERYLSSALFKELREEAENMQPVPVE
Sbjct: 361 SFGERYLSSALFKELREEAENMQPVPVE 388

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BLAST of Spo01586.1 vs. NCBI nr
Match: gi|731311581|ref|XP_010670142.1| (PREDICTED: bifunctional L-3-cyanoalanine synthase/cysteine synthase, mitochondrial [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 630.6 bits (1625), Expect = 1.800e-177
Identity = 325/369 (88.08%), Postives = 354/369 (95.93%), Query Frame = 1


		  
Query: 1   MATVSSCLLRRSRTASRIFKTS-LRCFSTTSSSAQTVSGSSPFPFTGTNIKTNVSQLIGR 60
           MATVSS ++RRSRT S+IFK++ +RCFS++S ++QTV+ S+   F  T IKT+VSQL+GR
Sbjct: 1   MATVSSFMMRRSRTNSQIFKSAVVRCFSSSSDASQTVADSAFSSFPPT-IKTHVSQLVGR 60

Query: 61  TPLVYLSKISEGSGAYIAVKQEMMQPTASVKDRPALAMIEDAEKKGLISPGKTVLIEPTS 120
           TPLV+L+K++EG GAYIAVKQEMMQPT+SVKDRPALAM+EDAEKKGLISPGKTVLIEPTS
Sbjct: 61  TPLVFLNKVTEGCGAYIAVKQEMMQPTSSVKDRPALAMLEDAEKKGLISPGKTVLIEPTS 120

Query: 121 GNMGISMAFMAAMKGYKMVLTMPSYTSMERRVVMRAFGADLILTDPDKGMGGTVKKANQL 180
           GNMGISMAFMAAMKGYKMVLTMPSYTSMERRV MRAFGA+LILTDPDKGMGGTVKKA QL
Sbjct: 121 GNMGISMAFMAAMKGYKMVLTMPSYTSMERRVTMRAFGAELILTDPDKGMGGTVKKAYQL 180

Query: 181 LDSTPDGFMLQQFNNPANTQVHFETTGPEIWEDTQGKVDIFVMGIGSGGTVSGVGRYLKS 240
           LDSTPDGFMLQQF+NPANTQVHFETTGPEIWEDTQGKVDIFVMGIGSGGTVSGVG+YLKS
Sbjct: 181 LDSTPDGFMLQQFSNPANTQVHFETTGPEIWEDTQGKVDIFVMGIGSGGTVSGVGQYLKS 240

Query: 241 QNPNVKIYGVEPAESNILNGGKPGPHLITGNGVGFKPEILDMDVMDAVLEVKSDDAVKMA 300
           QNPNVKIYGVEPAESNILNGGKPGPHLITGNGVGFKPEILDMDVM+AVLEVKSDDA+KMA
Sbjct: 241 QNPNVKIYGVEPAESNILNGGKPGPHLITGNGVGFKPEILDMDVMEAVLEVKSDDAIKMA 300

Query: 301 RQLALQEGLLVGISSGANTIAALDLAKRPENKGKLIVTIHPSFGERYLSSALFKELREEA 360
           RQLAL+EGL+VGISSGANT+AAL+LAKRPENKGKLIVTIHPSFGERYLSSALFKELREEA
Sbjct: 301 RQLALKEGLMVGISSGANTVAALELAKRPENKGKLIVTIHPSFGERYLSSALFKELREEA 360

Query: 361 ENMQPVPVE 369
           E MQPVPVE
Sbjct: 361 EQMQPVPVE 368

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BLAST of Spo01586.1 vs. NCBI nr
Match: gi|747045159|ref|XP_011092647.1| (PREDICTED: bifunctional L-3-cyanoalanine synthase/cysteine synthase 1, mitochondrial [Sesamum indicum])

HSP 1 Score: 559.7 bits (1441), Expect = 3.800e-156
Identity = 291/376 (77.39%), Postives = 325/376 (86.44%), Query Frame = 1


		  
Query: 1   MATVSSCLLRRSRTASRIFKTSLRCFSTTSSSAQTVSGSS--------PFPFTGTNIKTN 60
           MA +S+ + +RS  +  IFK     FSTT+S++  V   S        P    GTNIK  
Sbjct: 1   MAALSNLMKKRSHFS--IFKEKF--FSTTASASPLVDSPSFAQRLRDLPKDLPGTNIKKE 60

Query: 61  VSQLIGRTPLVYLSKISEGSGAYIAVKQEMMQPTASVKDRPALAMIEDAEKKGLISPGKT 120
           VSQLIGRTPLVYL+K++EG GAY+AVKQEMMQPTAS+KDRPA AMI DAEKKGLI+PGKT
Sbjct: 61  VSQLIGRTPLVYLNKVTEGCGAYVAVKQEMMQPTASIKDRPAYAMITDAEKKGLITPGKT 120

Query: 121 VLIEPTSGNMGISMAFMAAMKGYKMVLTMPSYTSMERRVVMRAFGADLILTDPDKGMGGT 180
            LIEPTSGNMGISMAFMAAMKGYKMVLTMPSYTS+ERRV MRAFGADLILTDP KGMGGT
Sbjct: 121 TLIEPTSGNMGISMAFMAAMKGYKMVLTMPSYTSLERRVTMRAFGADLILTDPTKGMGGT 180

Query: 181 VKKANQLLDSTPDGFMLQQFNNPANTQVHFETTGPEIWEDTQGKVDIFVMGIGSGGTVSG 240
           VKKA +LL+STPD FMLQQF+NPANTQVHFETTGPEIWEDT GKVDIF+MGIGSGGTVSG
Sbjct: 181 VKKAYELLESTPDAFMLQQFSNPANTQVHFETTGPEIWEDTNGKVDIFIMGIGSGGTVSG 240

Query: 241 VGRYLKSQNPNVKIYGVEPAESNILNGGKPGPHLITGNGVGFKPEILDMDVMDAVLEVKS 300
           VG+YLKS+NP+VKIYG+EP ESNILNGGKPGPH ITGNGVGFKP+ILDMDVM+ VL V S
Sbjct: 241 VGQYLKSKNPDVKIYGLEPEESNILNGGKPGPHHITGNGVGFKPDILDMDVMEEVLMVSS 300

Query: 301 DDAVKMARQLALQEGLLVGISSGANTIAALDLAKRPENKGKLIVTIHPSFGERYLSSALF 360
           +DAV MAR+LAL+EGL+VGISSGANT+AAL LA+RPENKGKLIVT+HPSFGERYLSS LF
Sbjct: 301 EDAVNMARELALKEGLMVGISSGANTVAALRLARRPENKGKLIVTVHPSFGERYLSSVLF 360

Query: 361 KELREEAENMQPVPVE 369
           +ELR EAE MQPV V+
Sbjct: 361 QELRNEAEKMQPVSVD 372

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BLAST of Spo01586.1 vs. NCBI nr
Match: gi|470142107|ref|XP_004306760.1| (PREDICTED: L-3-cyanoalanine synthase 2, mitochondrial [Fragaria vesca subsp. vesca])

HSP 1 Score: 557.4 bits (1435), Expect = 1.900e-155
Identity = 291/377 (77.19%), Postives = 327/377 (86.74%), Query Frame = 1


		  
Query: 1   MATVSSCLLRRSRTASRIFKTSLRCFSTTSSSAQTVSGSSPF---------PFTGTNIKT 60
           MA+++S L +RS  +  +   +LR     S++AQ ++ SS F            GT+IKT
Sbjct: 1   MASLTSFLKKRSTLS--VCNEALRMKRLFSTTAQPIADSSSFVERIRNLPKALPGTHIKT 60

Query: 61  NVSQLIGRTPLVYLSKISEGSGAYIAVKQEMMQPTASVKDRPALAMIEDAEKKGLISPGK 120
            VSQLIGRTPLVYL+K++EG GAYIAVKQEM QPTAS+KDRPALAM  DAEKKGLI+PG 
Sbjct: 61  EVSQLIGRTPLVYLNKVTEGCGAYIAVKQEMFQPTASIKDRPALAMFNDAEKKGLITPGV 120

Query: 121 TVLIEPTSGNMGISMAFMAAMKGYKMVLTMPSYTSMERRVVMRAFGADLILTDPDKGMGG 180
           T LIEPTSGNMGISMAFMAAMKGYKMVLTMPSYTS+ERRV MRAFGA+LILTDP KGMGG
Sbjct: 121 TTLIEPTSGNMGISMAFMAAMKGYKMVLTMPSYTSLERRVCMRAFGAELILTDPTKGMGG 180

Query: 181 TVKKANQLLDSTPDGFMLQQFNNPANTQVHFETTGPEIWEDTQGKVDIFVMGIGSGGTVS 240
           TVKKA  LL+STP+  MLQQF+NPANTQVHFETTGPEIWEDT GKVDIFVMGIGSGGTVS
Sbjct: 181 TVKKAYDLLESTPNALMLQQFSNPANTQVHFETTGPEIWEDTDGKVDIFVMGIGSGGTVS 240

Query: 241 GVGRYLKSQNPNVKIYGVEPAESNILNGGKPGPHLITGNGVGFKPEILDMDVMDAVLEVK 300
           GVG+YLKSQNP+ KIYGVEPAESNILNGGKPGPH ITGNGVGFKP+ILDMDVM+ VLEV+
Sbjct: 241 GVGQYLKSQNPDCKIYGVEPAESNILNGGKPGPHSITGNGVGFKPDILDMDVMERVLEVR 300

Query: 301 SDDAVKMARQLALQEGLLVGISSGANTIAALDLAKRPENKGKLIVTIHPSFGERYLSSAL 360
           S+DAV MARQLAL+EGL+VGISSGANT+AAL+LAK+PENKGKLIVTIH SFGERYLSS L
Sbjct: 301 SEDAVNMARQLALKEGLMVGISSGANTVAALELAKKPENKGKLIVTIHASFGERYLSSVL 360

Query: 361 FKELREEAENMQPVPVE 369
           F+ELR+EAENMQPV V+
Sbjct: 361 FEELRKEAENMQPVSVD 375

[Close]

BLAST of Spo01586.1 vs. UniProtKB/TrEMBL
Match: A0A0K9R1K5_SPIOL (Cysteine synthase OS=Spinacia oleracea GN=SOVF_117360 PE=3 SV=1)

HSP 1 Score: 699.9 bits (1805), Expect = 1.700e-198
Identity = 368/388 (94.85%), Postives = 368/388 (94.85%), Query Frame = 1


		  
Query: 1   MATVSSCLLRRSRTASRIFKTSLRCFSTTSSSAQTVSGSSPFPFTGTNIKTNVSQLIGRT 60
           MATVSSCLLRRSRTASRIFKTSLRCFSTTSSSAQTVSGSSPFPFTGTNIKTNVSQLIGRT
Sbjct: 1   MATVSSCLLRRSRTASRIFKTSLRCFSTTSSSAQTVSGSSPFPFTGTNIKTNVSQLIGRT 60

Query: 61  PLVYLSKISEGSGAYIAVKQEMMQPTASVKDRPALAMIEDAEKKGLISPGKTVLIEPTSG 120
           PLVYLSKISEGSGAYIAVKQEMMQPTASVKDRPALAMIEDAEKKGLISPGKTVLIEPTSG
Sbjct: 61  PLVYLSKISEGSGAYIAVKQEMMQPTASVKDRPALAMIEDAEKKGLISPGKTVLIEPTSG 120

Query: 121 NMGISMAFMAAMKGYKMVLTMPSYTSMERRVVMRAFGADLILTDPDKGMGGTVKKANQLL 180
           NMGISMAFMAAMKGYKMVLTMPSYTSMERRVVMRAFGADLILTDPDKGMGGTVKKANQLL
Sbjct: 121 NMGISMAFMAAMKGYKMVLTMPSYTSMERRVVMRAFGADLILTDPDKGMGGTVKKANQLL 180

Query: 181 DSTPDGFMLQQFNNPANTQVHFETTGPEIWEDTQGKVDIFVMGIGSGGTVSGVGRYLKSQ 240
           DSTPDGFMLQQFNNPANTQVHFETTGPEIWEDTQGKVDIFVMGIGSGGTVSGVGRYLKSQ
Sbjct: 181 DSTPDGFMLQQFNNPANTQVHFETTGPEIWEDTQGKVDIFVMGIGSGGTVSGVGRYLKSQ 240

Query: 241 NPNVKIYGVEPAESNILNGGKPGPHLITGNGVGFKPEILDMDVMDAVLE----------- 300
           NPNVKIYGVEPAESNILNGGKPGPHLITGNGVGFKPEILDMDVMDAVLE           
Sbjct: 241 NPNVKIYGVEPAESNILNGGKPGPHLITGNGVGFKPEILDMDVMDAVLEVKSDDAVKMAR 300

Query: 301 ---------VKSDDAVKMARQLALQEGLLVGISSGANTIAALDLAKRPENKGKLIVTIHP 360
                    VKSDDAVKMARQLALQEGLLVGISSGANTIAALDLAKRPENKGKLIVTIHP
Sbjct: 301 QLALQEGLLVKSDDAVKMARQLALQEGLLVGISSGANTIAALDLAKRPENKGKLIVTIHP 360

Query: 361 SFGERYLSSALFKELREEAENMQPVPVE 369
           SFGERYLSSALFKELREEAENMQPVPVE
Sbjct: 361 SFGERYLSSALFKELREEAENMQPVPVE 388

[Close]

BLAST of Spo01586.1 vs. UniProtKB/TrEMBL
Match: A0A0J8D7W0_BETVU (Cysteine synthase OS=Beta vulgaris subsp. vulgaris GN=BVRB_1g004430 PE=3 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 1.200e-177
Identity = 325/369 (88.08%), Postives = 354/369 (95.93%), Query Frame = 1


		  
Query: 1   MATVSSCLLRRSRTASRIFKTS-LRCFSTTSSSAQTVSGSSPFPFTGTNIKTNVSQLIGR 60
           MATVSS ++RRSRT S+IFK++ +RCFS++S ++QTV+ S+   F  T IKT+VSQL+GR
Sbjct: 1   MATVSSFMMRRSRTNSQIFKSAVVRCFSSSSDASQTVADSAFSSFPPT-IKTHVSQLVGR 60

Query: 61  TPLVYLSKISEGSGAYIAVKQEMMQPTASVKDRPALAMIEDAEKKGLISPGKTVLIEPTS 120
           TPLV+L+K++EG GAYIAVKQEMMQPT+SVKDRPALAM+EDAEKKGLISPGKTVLIEPTS
Sbjct: 61  TPLVFLNKVTEGCGAYIAVKQEMMQPTSSVKDRPALAMLEDAEKKGLISPGKTVLIEPTS 120

Query: 121 GNMGISMAFMAAMKGYKMVLTMPSYTSMERRVVMRAFGADLILTDPDKGMGGTVKKANQL 180
           GNMGISMAFMAAMKGYKMVLTMPSYTSMERRV MRAFGA+LILTDPDKGMGGTVKKA QL
Sbjct: 121 GNMGISMAFMAAMKGYKMVLTMPSYTSMERRVTMRAFGAELILTDPDKGMGGTVKKAYQL 180

Query: 181 LDSTPDGFMLQQFNNPANTQVHFETTGPEIWEDTQGKVDIFVMGIGSGGTVSGVGRYLKS 240
           LDSTPDGFMLQQF+NPANTQVHFETTGPEIWEDTQGKVDIFVMGIGSGGTVSGVG+YLKS
Sbjct: 181 LDSTPDGFMLQQFSNPANTQVHFETTGPEIWEDTQGKVDIFVMGIGSGGTVSGVGQYLKS 240

Query: 241 QNPNVKIYGVEPAESNILNGGKPGPHLITGNGVGFKPEILDMDVMDAVLEVKSDDAVKMA 300
           QNPNVKIYGVEPAESNILNGGKPGPHLITGNGVGFKPEILDMDVM+AVLEVKSDDA+KMA
Sbjct: 241 QNPNVKIYGVEPAESNILNGGKPGPHLITGNGVGFKPEILDMDVMEAVLEVKSDDAIKMA 300

Query: 301 RQLALQEGLLVGISSGANTIAALDLAKRPENKGKLIVTIHPSFGERYLSSALFKELREEA 360
           RQLAL+EGL+VGISSGANT+AAL+LAKRPENKGKLIVTIHPSFGERYLSSALFKELREEA
Sbjct: 301 RQLALKEGLMVGISSGANTVAALELAKRPENKGKLIVTIHPSFGERYLSSALFKELREEA 360

Query: 361 ENMQPVPVE 369
           E MQPVPVE
Sbjct: 361 EQMQPVPVE 368

[Close]

BLAST of Spo01586.1 vs. UniProtKB/TrEMBL
Match: A0A166CYY9_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_005355 PE=4 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 3.900e-155
Identity = 275/328 (83.84%), Postives = 302/328 (92.07%), Query Frame = 1


		  
Query: 41  PFPFTGTNIKTNVSQLIGRTPLVYLSKISEGSGAYIAVKQEMMQPTASVKDRPALAMIED 100
           P     T I+  VSQLIG+TPLVYL+K++EG GAYIAVKQEMMQPT+S+KDRPALAMI D
Sbjct: 48  PKDLPSTKIRREVSQLIGKTPLVYLNKVTEGCGAYIAVKQEMMQPTSSIKDRPALAMIND 107

Query: 101 AEKKGLISPGKTVLIEPTSGNMGISMAFMAAMKGYKMVLTMPSYTSMERRVVMRAFGADL 160
           AEKK LI+PGKTVLIEPTSGNMGISMAFMAAMKGYKMVLTMPSYTS+ERRV M+AFGADL
Sbjct: 108 AEKKKLITPGKTVLIEPTSGNMGISMAFMAAMKGYKMVLTMPSYTSLERRVCMKAFGADL 167

Query: 161 ILTDPDKGMGGTVKKANQLLDSTPDGFMLQQFNNPANTQVHFETTGPEIWEDTQGKVDIF 220
           ILTDP KGMGGTVKKA  LL++TPDGFMLQQF+NPANTQ+HFETTGPEIWEDT G VDIF
Sbjct: 168 ILTDPTKGMGGTVKKAYDLLENTPDGFMLQQFSNPANTQIHFETTGPEIWEDTNGNVDIF 227

Query: 221 VMGIGSGGTVSGVGRYLKSQNPNVKIYGVEPAESNILNGGKPGPHLITGNGVGFKPEILD 280
           VMGIGSGGTVSGVG+YLKSQNPNVKIYG+EP ESN+LNGGKPGPH ITGNGVGFKP+ILD
Sbjct: 228 VMGIGSGGTVSGVGQYLKSQNPNVKIYGIEPTESNVLNGGKPGPHWITGNGVGFKPDILD 287

Query: 281 MDVMDAVLEVKSDDAVKMARQLALQEGLLVGISSGANTIAALDLAKRPENKGKLIVTIHP 340
           MDVMD VL V S DAV MAR+LAL+EGL+VGISSGANT+AAL+LAKRPENKGKLIVTIHP
Sbjct: 288 MDVMDEVLMVSSQDAVDMARKLALKEGLMVGISSGANTVAALELAKRPENKGKLIVTIHP 347

Query: 341 SFGERYLSSALFKELREEAENMQPVPVE 369
           SFGERYLSS LF+E+R+EAENMQPV V+
Sbjct: 348 SFGERYLSSVLFQEIRQEAENMQPVSVD 375

[Close]

BLAST of Spo01586.1 vs. UniProtKB/TrEMBL
Match: M5X090_PRUPE (Cysteine synthase OS=Prunus persica GN=PRUPE_ppa007292mg PE=3 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 5.000e-155
Identity = 279/363 (76.86%), Postives = 321/363 (88.43%), Query Frame = 1


		  
Query: 6   SCLLRRSRTASRIFKTSLRCFSTTSSSAQTVSGSSPFPFTGTNIKTNVSQLIGRTPLVYL 65
           S LL       R+F T +      S S      + P    GT++KT VSQLIGRTP+VYL
Sbjct: 12  STLLCNEAVRKRLFSTQVSQPIIDSPSFAQRVRNLPKDLPGTHVKTEVSQLIGRTPIVYL 71

Query: 66  SKISEGSGAYIAVKQEMMQPTASVKDRPALAMIEDAEKKGLISPGKTVLIEPTSGNMGIS 125
           +K++EG GAYIAVKQEM QPT+S+KDRPAL+MI DAEKKGLI+PGKTVL+EPTSGNMGIS
Sbjct: 72  NKVTEGCGAYIAVKQEMFQPTSSIKDRPALSMINDAEKKGLITPGKTVLVEPTSGNMGIS 131

Query: 126 MAFMAAMKGYKMVLTMPSYTSMERRVVMRAFGADLILTDPDKGMGGTVKKANQLLDSTPD 185
           MAFMAAM+GYKMVLTMPSYTS+ERRV MRAFGA+LILTDP +GMGGTVKKA  LL+STP+
Sbjct: 132 MAFMAAMRGYKMVLTMPSYTSLERRVCMRAFGAELILTDPARGMGGTVKKAYDLLESTPN 191

Query: 186 GFMLQQFNNPANTQVHFETTGPEIWEDTQGKVDIFVMGIGSGGTVSGVGRYLKSQNPNVK 245
             MLQQF+NPANT+VHFETTGPEIWEDT G+VDIF+MGIGSGGTVSGVG+YLKS+NPNV+
Sbjct: 192 AHMLQQFSNPANTRVHFETTGPEIWEDTDGQVDIFIMGIGSGGTVSGVGQYLKSKNPNVQ 251

Query: 246 IYGVEPAESNILNGGKPGPHLITGNGVGFKPEILDMDVMDAVLEVKSDDAVKMARQLALQ 305
           IYGVEPAESN+LNGGKPGPHLITGNGVGFKP+ILD+DVMD V+EVKSDDAVKMAR+LAL+
Sbjct: 252 IYGVEPAESNVLNGGKPGPHLITGNGVGFKPDILDLDVMDRVIEVKSDDAVKMARRLALE 311

Query: 306 EGLLVGISSGANTIAALDLAKRPENKGKLIVTIHPSFGERYLSSALFKELREEAENMQPV 365
           EGL+VGISSGANT+AA++LAK+PENKGKLIVT+HPSFGERYLSS LF+ELR+EAENMQPV
Sbjct: 312 EGLMVGISSGANTVAAIELAKKPENKGKLIVTVHPSFGERYLSSVLFEELRKEAENMQPV 371

Query: 366 PVE 369
            V+
Sbjct: 372 SVD 374

[Close]

BLAST of Spo01586.1 vs. UniProtKB/TrEMBL
Match: A5AEP0_VITVI (Cysteine synthase OS=Vitis vinifera GN=VITISV_028023 PE=3 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 8.600e-155
Identity = 294/375 (78.40%), Postives = 323/375 (86.13%), Query Frame = 1


		  
Query: 1   MATVSSCLLRRSRTASRIFKTSL---RCFSTT----SSSAQTVSGSSPFPFTGTNIKTNV 60
           MA +SS   R+S     I  TSL   R FST     S S        P    GTNIK  V
Sbjct: 1   MAALSSFFKRKSS----IPFTSLAIRRWFSTQPILDSPSFSQRIRDLPKDLPGTNIKKEV 60

Query: 61  SQLIGRTPLVYLSKISEGSGAYIAVKQEMMQPTASVKDRPALAMIEDAEKKGLISPGKTV 120
           SQLIG+TPLV+L+K++EG GAY+AVKQEMMQPTAS+KDRPALAMI DAE K LI+PGKTV
Sbjct: 61  SQLIGKTPLVFLNKVTEGCGAYVAVKQEMMQPTASIKDRPALAMITDAEBKQLITPGKTV 120

Query: 121 LIEPTSGNMGISMAFMAAMKGYKMVLTMPSYTSMERRVVMRAFGADLILTDPDKGMGGTV 180
           LIEPTSGNMGISMAFMAAMKGYKMVLTMPSYTS+ERRV MRAFGADLILTDP KGMGGTV
Sbjct: 121 LIEPTSGNMGISMAFMAAMKGYKMVLTMPSYTSLERRVTMRAFGADLILTDPTKGMGGTV 180

Query: 181 KKANQLLDSTPDGFMLQQFNNPANTQVHFETTGPEIWEDTQGKVDIFVMGIGSGGTVSGV 240
           KKA +LL+STPD FMLQQF+NPANTQVHFETTGPEIWEDT+G+VDIF+MGIGSGGTVSGV
Sbjct: 181 KKAYELLESTPDAFMLQQFSNPANTQVHFETTGPEIWEDTRGQVDIFIMGIGSGGTVSGV 240

Query: 241 GRYLKSQNPNVKIYGVEPAESNILNGGKPGPHLITGNGVGFKPEILDMDVMDAVLEVKSD 300
           GRYLKSQNPNVKIYG+EP ESN+LNGGKPGPH ITGNGVGFKP+ILDMDVM+ VL V S+
Sbjct: 241 GRYLKSQNPNVKIYGLEPTESNVLNGGKPGPHHITGNGVGFKPDILDMDVMEEVLMVSSE 300

Query: 301 DAVKMARQLALQEGLLVGISSGANTIAALDLAKRPENKGKLIVTIHPSFGERYLSSALFK 360
           DAV MARQLAL+EGL+VGISSGANT+AAL LA+RPENKGKLIVTIHPSFGERYLSS LF+
Sbjct: 301 DAVNMARQLALKEGLMVGISSGANTVAALRLARRPENKGKLIVTIHPSFGERYLSSVLFQ 360

Query: 361 ELREEAENMQPVPVE 369
           ELR+EAENMQPV V+
Sbjct: 361 ELRKEAENMQPVSVD 371

[Close]

BLAST of Spo01586.1 vs. ExPASy Swiss-Prot
Match: CYSC_SPIOL (Bifunctional L-3-cyanoalanine synthase/cysteine synthase, mitochondrial OS=Spinacia oleracea GN=CYSC PE=1 SV=1)

HSP 1 Score: 711.8 bits (1836), Expect = 3.800e-204
Identity = 368/368 (100.00%), Postives = 368/368 (100.00%), Query Frame = 1


		  
Query: 1   MATVSSCLLRRSRTASRIFKTSLRCFSTTSSSAQTVSGSSPFPFTGTNIKTNVSQLIGRT 60
           MATVSSCLLRRSRTASRIFKTSLRCFSTTSSSAQTVSGSSPFPFTGTNIKTNVSQLIGRT
Sbjct: 1   MATVSSCLLRRSRTASRIFKTSLRCFSTTSSSAQTVSGSSPFPFTGTNIKTNVSQLIGRT 60

Query: 61  PLVYLSKISEGSGAYIAVKQEMMQPTASVKDRPALAMIEDAEKKGLISPGKTVLIEPTSG 120
           PLVYLSKISEGSGAYIAVKQEMMQPTASVKDRPALAMIEDAEKKGLISPGKTVLIEPTSG
Sbjct: 61  PLVYLSKISEGSGAYIAVKQEMMQPTASVKDRPALAMIEDAEKKGLISPGKTVLIEPTSG 120

Query: 121 NMGISMAFMAAMKGYKMVLTMPSYTSMERRVVMRAFGADLILTDPDKGMGGTVKKANQLL 180
           NMGISMAFMAAMKGYKMVLTMPSYTSMERRVVMRAFGADLILTDPDKGMGGTVKKANQLL
Sbjct: 121 NMGISMAFMAAMKGYKMVLTMPSYTSMERRVVMRAFGADLILTDPDKGMGGTVKKANQLL 180

Query: 181 DSTPDGFMLQQFNNPANTQVHFETTGPEIWEDTQGKVDIFVMGIGSGGTVSGVGRYLKSQ 240
           DSTPDGFMLQQFNNPANTQVHFETTGPEIWEDTQGKVDIFVMGIGSGGTVSGVGRYLKSQ
Sbjct: 181 DSTPDGFMLQQFNNPANTQVHFETTGPEIWEDTQGKVDIFVMGIGSGGTVSGVGRYLKSQ 240

Query: 241 NPNVKIYGVEPAESNILNGGKPGPHLITGNGVGFKPEILDMDVMDAVLEVKSDDAVKMAR 300
           NPNVKIYGVEPAESNILNGGKPGPHLITGNGVGFKPEILDMDVMDAVLEVKSDDAVKMAR
Sbjct: 241 NPNVKIYGVEPAESNILNGGKPGPHLITGNGVGFKPEILDMDVMDAVLEVKSDDAVKMAR 300

Query: 301 QLALQEGLLVGISSGANTIAALDLAKRPENKGKLIVTIHPSFGERYLSSALFKELREEAE 360
           QLALQEGLLVGISSGANTIAALDLAKRPENKGKLIVTIHPSFGERYLSSALFKELREEAE
Sbjct: 301 QLALQEGLLVGISSGANTIAALDLAKRPENKGKLIVTIHPSFGERYLSSALFKELREEAE 360

Query: 361 NMQPVPVE 369
           NMQPVPVE
Sbjct: 361 NMQPVPVE 368

[Close]

BLAST of Spo01586.1 vs. ExPASy Swiss-Prot
Match: CAS1_SOLTU (Bifunctional L-3-cyanoalanine synthase/cysteine synthase 1, mitochondrial OS=Solanum tuberosum GN=PCAS-1 PE=1 SV=1)

HSP 1 Score: 546.2 bits (1406), Expect = 2.700e-154
Identity = 272/345 (78.84%), Postives = 309/345 (89.57%), Query Frame = 1


		  
Query: 24  RCFSTTSSSAQTVSGSSPFPFTGTNIKTNVSQLIGRTPLVYLSKISEGSGAYIAVKQEMM 83
           R +S+ SS AQ +    P    GTNIKT VSQLIG+TPLVYL+K+SEG GAYIAVKQEMM
Sbjct: 8   RFYSSESSFAQRLR-DLPKYLPGTNIKTQVSQLIGKTPLVYLNKVSEGCGAYIAVKQEMM 67

Query: 84  QPTASVKDRPALAMIEDAEKKGLISPGKTVLIEPTSGNMGISMAFMAAMKGYKMVLTMPS 143
           QPT+S+KDRPA AMI DAEKKGLI+PGKT LIEPTSGNMGISMAFMAAMKGYKM+LTMPS
Sbjct: 68  QPTSSIKDRPAFAMINDAEKKGLITPGKTTLIEPTSGNMGISMAFMAAMKGYKMILTMPS 127

Query: 144 YTSMERRVVMRAFGADLILTDPDKGMGGTVKKANQLLDSTPDGFMLQQFNNPANTQVHFE 203
           YTS+ERRV MRAFGADL+ TDP KGMGGT+KKA  LL+STP+ +MLQQF+NPANTQ HFE
Sbjct: 128 YTSLERRVTMRAFGADLVTTDPTKGMGGTIKKAYDLLESTPNAYMLQQFSNPANTQAHFE 187

Query: 204 TTGPEIWEDTQGKVDIFVMGIGSGGTVSGVGRYLKSQNPNVKIYGVEPAESNILNGGKPG 263
           TTGPEIWEDTQG VDIFVMGIGSGGTVSGVG+YLKS+NPNVKIYG+EP ESN+LNGG PG
Sbjct: 188 TTGPEIWEDTQGNVDIFVMGIGSGGTVSGVGQYLKSKNPNVKIYGIEPTESNVLNGGNPG 247

Query: 264 PHLITGNGVGFKPEILDMDVMDAVLEVKSDDAVKMARQLALQEGLLVGISSGANTIAALD 323
           PH ITGNGVGFKP+ILDMDVM+ VL V S+++V MAR+LAL+EGL+VGISSGANT+AAL 
Sbjct: 248 PHEITGNGVGFKPDILDMDVMEEVLMVSSEESVNMARELALKEGLMVGISSGANTVAALR 307

Query: 324 LAKRPENKGKLIVTIHPSFGERYLSSALFKELREEAENMQPVPVE 369
           LA RPENKGKLIVTIHPSFGERYLSS L++++R+EA+NMQPV V+
Sbjct: 308 LANRPENKGKLIVTIHPSFGERYLSSVLYEDIRKEAQNMQPVSVD 351

[Close]

BLAST of Spo01586.1 vs. ExPASy Swiss-Prot
Match: CAS1_MALDO (L-3-cyanoalanine synthase 1, mitochondrial OS=Malus domestica GN=CAS1 PE=1 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 1.000e-153
Identity = 277/374 (74.06%), Postives = 322/374 (86.10%), Query Frame = 1


		  
Query: 1   MATVSSCLLRRSRTAS------RIFKTSLRCFSTTSSSAQTVSGSSPFPFTGTNIKTNVS 60
           MA + S L +RS          ++F T +    T S S      + P    GT IKT VS
Sbjct: 1   MAALRSFLKKRSSVLCNGAMRRKLFSTEVSQPLTDSPSFAQRIRNLPKDLPGTQIKTQVS 60

Query: 61  QLIGRTPLVYLSKISEGSGAYIAVKQEMMQPTASVKDRPALAMIEDAEKKGLISPGKTVL 120
           QLIGRTP+VYL+K++EG GAYIAVKQEM QPTAS+KDRPAL+MI DAE+KGLI+PG+T+L
Sbjct: 61  QLIGRTPIVYLNKVTEGCGAYIAVKQEMFQPTASIKDRPALSMINDAEEKGLITPGETIL 120

Query: 121 IEPTSGNMGISMAFMAAMKGYKMVLTMPSYTSMERRVVMRAFGADLILTDPDKGMGGTVK 180
           IEPTSGNMGISMAFMAAM+GYKMVLTMPSYTS+ERRV MR FGADLILTDP KGMGGTVK
Sbjct: 121 IEPTSGNMGISMAFMAAMRGYKMVLTMPSYTSLERRVCMRCFGADLILTDPTKGMGGTVK 180

Query: 181 KANQLLDSTPDGFMLQQFNNPANTQVHFETTGPEIWEDTQGKVDIFVMGIGSGGTVSGVG 240
           KA  LL+STP+ +MLQQF+NPANT+VHFETTGPEIWEDT G+VDIFVMGIGSGGTVSGVG
Sbjct: 181 KAYDLLESTPNAYMLQQFSNPANTKVHFETTGPEIWEDTNGQVDIFVMGIGSGGTVSGVG 240

Query: 241 RYLKSQNPNVKIYGVEPAESNILNGGKPGPHLITGNGVGFKPEILDMDVMDAVLEVKSDD 300
           +YLKS+NPNV+IYGVEPAESN+LNGGKPGPH I GNGVGFKP+ILD+D+++ V+EV S+D
Sbjct: 241 QYLKSKNPNVQIYGVEPAESNVLNGGKPGPHSIMGNGVGFKPDILDLDMLERVIEVTSED 300

Query: 301 AVKMARQLALQEGLLVGISSGANTIAALDLAKRPENKGKLIVTIHPSFGERYLSSALFKE 360
           AV MARQLAL+EGL+VGISSGANT+AA++LAK+PENKGKLIVT+HPSFGERYLSS LF+E
Sbjct: 301 AVNMARQLALKEGLMVGISSGANTVAAMELAKKPENKGKLIVTVHPSFGERYLSSVLFQE 360

Query: 361 LREEAENMQPVPVE 369
           LR+EAENMQPV V+
Sbjct: 361 LRQEAENMQPVAVD 374

[Close]

BLAST of Spo01586.1 vs. ExPASy Swiss-Prot
Match: CAS2_MALDO (L-3-cyanoalanine synthase 2, mitochondrial OS=Malus domestica GN=CAS2 PE=1 SV=1)

HSP 1 Score: 543.9 bits (1400), Expect = 1.400e-153
Identity = 275/364 (75.55%), Postives = 315/364 (86.54%), Query Frame = 1


		  
Query: 5   SSCLLRRSRTASRIFKTSLRCFSTTSSSAQTVSGSSPFPFTGTNIKTNVSQLIGRTPLVY 64
           SS L        R+F   +      S S     G+ P     T IKT VSQLIGRTP+VY
Sbjct: 12  SSVLCNGGAVRHRLFSAQVSQPIPDSPSFAQRIGNLPKDLPATQIKTQVSQLIGRTPIVY 71

Query: 65  LSKISEGSGAYIAVKQEMMQPTASVKDRPALAMIEDAEKKGLISPGKTVLIEPTSGNMGI 124
           L+K++EG GA+IAVKQEM QPTAS+KDRPAL+MI DAE+KGLI+PG+T LIEPTSGNMGI
Sbjct: 72  LNKVTEGCGAFIAVKQEMFQPTASIKDRPALSMINDAEEKGLITPGETTLIEPTSGNMGI 131

Query: 125 SMAFMAAMKGYKMVLTMPSYTSMERRVVMRAFGADLILTDPDKGMGGTVKKANQLLDSTP 184
           SMAFMAAMKGYKMVLTMPSYTS+ERRV MR FGADLILTDP KGMGGTVKKA  LL+STP
Sbjct: 132 SMAFMAAMKGYKMVLTMPSYTSLERRVCMRCFGADLILTDPTKGMGGTVKKAYDLLESTP 191

Query: 185 DGFMLQQFNNPANTQVHFETTGPEIWEDTQGKVDIFVMGIGSGGTVSGVGRYLKSQNPNV 244
           + FMLQQF+NPANT+VHFETTGPEIWEDT G+VDIFVMGIGSGGTVSGVG+YLKS+NPNV
Sbjct: 192 NAFMLQQFSNPANTKVHFETTGPEIWEDTNGQVDIFVMGIGSGGTVSGVGQYLKSKNPNV 251

Query: 245 KIYGVEPAESNILNGGKPGPHLITGNGVGFKPEILDMDVMDAVLEVKSDDAVKMARQLAL 304
           +IYGVEPAESN+LNGGKPGPH ITGNGVGFKP+ILDMD+M+ V+EV+S+DAV MARQLAL
Sbjct: 252 QIYGVEPAESNVLNGGKPGPHSITGNGVGFKPDILDMDMMERVIEVRSEDAVNMARQLAL 311

Query: 305 QEGLLVGISSGANTIAALDLAKRPENKGKLIVTIHPSFGERYLSSALFKELREEAENMQP 364
           +EGL+VGISSGANT+AA++LAK+PENKGKLIVT+H SFGERYLSS LF++LR+EAENMQP
Sbjct: 312 KEGLMVGISSGANTVAAMELAKKPENKGKLIVTVHASFGERYLSSVLFQDLRQEAENMQP 371

Query: 365 VPVE 369
           V V+
Sbjct: 372 VAVD 375

[Close]

BLAST of Spo01586.1 vs. ExPASy Swiss-Prot
Match: CAS2_SOLTU (Bifunctional L-3-cyanoalanine synthase/cysteine synthase 2, mitochondrial OS=Solanum tuberosum GN=PCAS-2 PE=1 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 6.300e-151
Identity = 274/368 (74.46%), Postives = 313/368 (85.05%), Query Frame = 1


		  
Query: 1   MATVSSCLLRRSRTASRIFKTSLRCFSTTSSSAQTVSGSSPFPFTGTNIKTNVSQLIGRT 60
           MAT+S  L +RS  ++R+F T L                       TNIK+ VSQLIG+T
Sbjct: 1   MATLSRFLKKRSLASNRLFSTQL---------------------PHTNIKSEVSQLIGKT 60

Query: 61  PLVYLSKISEGSGAYIAVKQEMMQPTASVKDRPALAMIEDAEKKGLISPGKTVLIEPTSG 120
           P+VYL K++EG GAYIAVKQEM QPT+S+KDRPALAMI DAEKKGLISP KT LIEPTSG
Sbjct: 61  PMVYLKKVTEGCGAYIAVKQEMFQPTSSIKDRPALAMINDAEKKGLISPEKTTLIEPTSG 120

Query: 121 NMGISMAFMAAMKGYKMVLTMPSYTSMERRVVMRAFGADLILTDPDKGMGGTVKKANQLL 180
           NMGISMAFMAAMKGYKMVLTMPSYTSMERRV MRAFGADLILTDP KGMGGTVKKA +LL
Sbjct: 121 NMGISMAFMAAMKGYKMVLTMPSYTSMERRVTMRAFGADLILTDPTKGMGGTVKKAYELL 180

Query: 181 DSTPDGFMLQQFNNPANTQVHFETTGPEIWEDTQGKVDIFVMGIGSGGTVSGVGRYLKSQ 240
           +STP+ FMLQQF+NPANTQVHF+TTGPEIWE+T G VDIFVMGIGSGGTV+GVG YLKS+
Sbjct: 181 ESTPNAFMLQQFSNPANTQVHFDTTGPEIWEETLGNVDIFVMGIGSGGTVTGVGLYLKSK 240

Query: 241 NPNVKIYGVEPAESNILNGGKPGPHLITGNGVGFKPEILDMDVMDAVLEVKSDDAVKMAR 300
           NPNVKIYG+EP ESNILNGGKPGPH ITGNGVG KP+I+DMD+M+ VL V S+DAV MAR
Sbjct: 241 NPNVKIYGLEPTESNILNGGKPGPHHITGNGVGSKPDIVDMDLMEEVLMVSSEDAVNMAR 300

Query: 301 QLALQEGLLVGISSGANTIAALDLAKRPENKGKLIVTIHPSFGERYLSSALFKELREEAE 360
           +LA++EGL+VGISSGANT+AAL LA++PENKGKLIVT+H SFGERYLSS L+++LR+EAE
Sbjct: 301 ELAVKEGLMVGISSGANTVAALRLAQKPENKGKLIVTVHASFGERYLSSVLYQDLRKEAE 347

Query: 361 NMQPVPVE 369
           NMQPV V+
Sbjct: 361 NMQPVSVD 347

[Close]

BLAST of Spo01586.1 vs. TAIR (Arabidopsis)
Match: AT3G61440.1 (cysteine synthase C1)

HSP 1 Score: 526.2 bits (1354), Expect = 1.700e-149
Identity = 275/370 (74.32%), Postives = 311/370 (84.05%), Query Frame = 1


		  
Query: 1   MATVSSCLLRRSRTASRIFKTSLRCFSTTSSS--AQTVSGSSPFPFTGTNIKTNVSQLIG 60
           MA+VS  LLRR  T      T  + FST  S   AQ +    P  F  TN K + S LIG
Sbjct: 1   MASVSRRLLRRE-TIPCFSHTVRKLFSTVGSPSFAQRLR-DLPKDFPSTNAKRDASLLIG 60

Query: 61  RTPLVYLSKISEGSGAYIAVKQEMMQPTASVKDRPALAMIEDAEKKGLISPGKTVLIEPT 120
           +TPLV+L+K++EG  AY+A KQE  QPT S+KDRPA+AMI DAEKK LI PGKT LIEPT
Sbjct: 61  KTPLVFLNKVTEGCEAYVAAKQEHFQPTCSIKDRPAIAMIADAEKKKLIIPGKTTLIEPT 120

Query: 121 SGNMGISMAFMAAMKGYKMVLTMPSYTSMERRVVMRAFGADLILTDPDKGMGGTVKKANQ 180
           SGNMGIS+AFMAAMKGY++++TMPSYTS+ERRV MR+FGA+L+LTDP KGMGGTVKKA  
Sbjct: 121 SGNMGISLAFMAAMKGYRIIMTMPSYTSLERRVTMRSFGAELVLTDPAKGMGGTVKKAYD 180

Query: 181 LLDSTPDGFMLQQFNNPANTQVHFETTGPEIWEDTQGKVDIFVMGIGSGGTVSGVGRYLK 240
           LLDSTPD FM QQF NPANTQ+HF+TTGPEIWEDT G VDIFVMGIGSGGTVSGVGRYLK
Sbjct: 181 LLDSTPDAFMCQQFANPANTQIHFDTTGPEIWEDTLGNVDIFVMGIGSGGTVSGVGRYLK 240

Query: 241 SQNPNVKIYGVEPAESNILNGGKPGPHLITGNGVGFKPEILDMDVMDAVLEVKSDDAVKM 300
           S+NPNVKIYGVEPAESNILNGGKPGPH ITGNGVGFKPEILDMDVM++VLEV S+DA+KM
Sbjct: 241 SKNPNVKIYGVEPAESNILNGGKPGPHAITGNGVGFKPEILDMDVMESVLEVSSEDAIKM 300

Query: 301 ARQLALQEGLLVGISSGANTIAALDLAKRPENKGKLIVTIHPSFGERYLSSALFKELREE 360
           AR+LAL+EGL+VGISSGANT+AA+ LAK PENKGKLIVTIH SFGERYLSS LF ELR+E
Sbjct: 301 ARELALKEGLMVGISSGANTVAAIRLAKMPENKGKLIVTIHASFGERYLSSVLFDELRKE 360

Query: 361 AENMQPVPVE 369
           AE M+PV V+
Sbjct: 361 AEEMKPVSVD 368

[Close]

BLAST of Spo01586.1 vs. TAIR (Arabidopsis)
Match: AT3G59760.1 (O-acetylserine (thiol) lyase isoform C)

HSP 1 Score: 422.5 bits (1085), Expect = 2.600e-118
Identity = 199/319 (62.38%), Postives = 261/319 (81.82%), Query Frame = 1


		  
Query: 46  GTNIKTNVSQLIGRTPLVYLSKISEGSGAYIAVKQEMMQPTASVKDRPALAMIEDAEKKG 105
           G NI  NVSQLIG+TP+VYL+ I++G  A IA K E+M+P  SVKDR   +M+ DAE+KG
Sbjct: 110 GLNIADNVSQLIGKTPMVYLNSIAKGCVANIAAKLEIMEPCCSVKDRIGYSMVTDAEQKG 169

Query: 106 LISPGKTVLIEPTSGNMGISMAFMAAMKGYKMVLTMPSYTSMERRVVMRAFGADLILTDP 165
            ISPGK+VL+EPTSGN GI +AF+AA +GY+++LTMP+  SMERRV+++AFGA+L+LTDP
Sbjct: 170 FISPGKSVLVEPTSGNTGIGLAFIAASRGYRLILTMPASMSMERRVLLKAFGAELVLTDP 229

Query: 166 DKGMGGTVKKANQLLDSTPDGFMLQQFNNPANTQVHFETTGPEIWEDTQGKVDIFVMGIG 225
            KGM G V+KA ++L +TPD +MLQQF+NPAN ++H+ETTGPEIW+DT+GKVDIFV GIG
Sbjct: 230 AKGMTGAVQKAEEILKNTPDAYMLQQFDNPANPKIHYETTGPEIWDDTKGKVDIFVAGIG 289

Query: 226 SGGTVSGVGRYLKSQNPNVKIYGVEPAESNILNGGKPGPHLITGNGVGFKPEILDMDVMD 285
           +GGT++GVGR++K +NP  ++ GVEP ES+IL+GGKPGPH I G G GF P+ LD  +MD
Sbjct: 290 TGGTITGVGRFIKEKNPKTQVIGVEPTESDILSGGKPGPHKIQGIGAGFIPKNLDQKIMD 349

Query: 286 AVLEVKSDDAVKMARQLALQEGLLVGISSGANTIAALDLAKRPENKGKLIVTIHPSFGER 345
            V+ + S++A++ A+QLAL+EGL+VGISSGA   AA+ +AKRPEN GKLI  + PSFGER
Sbjct: 350 EVIAISSEEAIETAKQLALKEGLMVGISSGAAAAAAIKVAKRPENAGKLIAVVFPSFGER 409

Query: 346 YLSSALFKELREEAENMQP 365
           YLS+ LF+ +REE E MQP
Sbjct: 410 YLSTPLFQSIREEVEKMQP 428

[Close]

BLAST of Spo01586.1 vs. TAIR (Arabidopsis)
Match: AT2G43750.1 (O-acetylserine (thiol) lyase B)

HSP 1 Score: 409.5 bits (1051), Expect = 2.300e-114
Identity = 192/319 (60.19%), Postives = 256/319 (80.25%), Query Frame = 1


		  
Query: 46  GTNIKTNVSQLIGRTPLVYLSKISEGSGAYIAVKQEMMQPTASVKDRPALAMIEDAEKKG 105
           G NI  N +QLIG+TP+VYL+ + +G  A +A K E+M+P  SVKDR   +MI DAE+KG
Sbjct: 72  GLNIADNAAQLIGKTPMVYLNNVVKGCVASVAAKLEIMEPCCSVKDRIGYSMITDAEEKG 131

Query: 106 LISPGKTVLIEPTSGNMGISMAFMAAMKGYKMVLTMPSYTSMERRVVMRAFGADLILTDP 165
           LI+PGK+VL+E TSGN GI +AF+AA KGYK++LTMP+  S+ERRV++RAFGA+L+LT+P
Sbjct: 132 LITPGKSVLVESTSGNTGIGLAFIAASKGYKLILTMPASMSLERRVLLRAFGAELVLTEP 191

Query: 166 DKGMGGTVKKANQLLDSTPDGFMLQQFNNPANTQVHFETTGPEIWEDTQGKVDIFVMGIG 225
            KGM G ++KA ++L  TP+ +MLQQF+NPAN ++H+ETTGPEIWEDT+GK+DI V GIG
Sbjct: 192 AKGMTGAIQKAEEILKKTPNSYMLQQFDNPANPKIHYETTGPEIWEDTRGKIDILVAGIG 251

Query: 226 SGGTVSGVGRYLKSQNPNVKIYGVEPAESNILNGGKPGPHLITGNGVGFKPEILDMDVMD 285
           +GGT++GVGR++K + P +K+ GVEP ES IL+GGKPGPH I G G GF P+ LD+ ++D
Sbjct: 252 TGGTITGVGRFIKERKPELKVIGVEPTESAILSGGKPGPHKIQGIGAGFVPKNLDLAIVD 311

Query: 286 AVLEVKSDDAVKMARQLALQEGLLVGISSGANTIAALDLAKRPENKGKLIVTIHPSFGER 345
             + + S++A++ ++QLALQEGLLVGISSGA   AA+ +AKRPEN GKLI  + PSFGER
Sbjct: 312 EYIAISSEEAIETSKQLALQEGLLVGISSGAAAAAAIQVAKRPENAGKLIAVVFPSFGER 371

Query: 346 YLSSALFKELREEAENMQP 365
           YLS+ LF+ +REE E MQP
Sbjct: 372 YLSTQLFQSIREECEQMQP 390

[Close]

BLAST of Spo01586.1 vs. TAIR (Arabidopsis)
Match: AT4G14880.1 (O-acetylserine (thiol) lyase (OAS-TL) isoform A1)

HSP 1 Score: 406.4 bits (1043), Expect = 1.900e-113
Identity = 195/316 (61.71%), Postives = 250/316 (79.11%), Query Frame = 1


		  
Query: 47  TNIKTNVSQLIGRTPLVYLSKISEGSGAYIAVKQEMMQPTASVKDRPALAMIEDAEKKGL 106
           + I  +V++LIG TPLVYL+ ++EG    +A K EMM+P +SVKDR   +MI DAEKKGL
Sbjct: 3   SRIAKDVTELIGNTPLVYLNNVAEGCVGRVAAKLEMMEPCSSVKDRIGFSMISDAEKKGL 62

Query: 107 ISPGKTVLIEPTSGNMGISMAFMAAMKGYKMVLTMPSYTSMERRVVMRAFGADLILTDPD 166
           I PG++VLIEPTSGN G+ +AF AA KGYK+++TMP+  S ERR+++ AFG +L+LTDP 
Sbjct: 63  IKPGESVLIEPTSGNTGVGLAFTAAAKGYKLIITMPASMSTERRIILLAFGVELVLTDPA 122

Query: 167 KGMGGTVKKANQLLDSTPDGFMLQQFNNPANTQVHFETTGPEIWEDTQGKVDIFVMGIGS 226
           KGM G + KA ++L  TP+G+MLQQF NPAN ++H+ETTGPEIW+ T GK+D FV GIG+
Sbjct: 123 KGMKGAIAKAEEILAKTPNGYMLQQFENPANPKIHYETTGPEIWKGTGGKIDGFVSGIGT 182

Query: 227 GGTVSGVGRYLKSQNPNVKIYGVEPAESNILNGGKPGPHLITGNGVGFKPEILDMDVMDA 286
           GGT++G G+YLK QN NVK+YGVEP ES IL+GGKPGPH I G G GF P +L++D++D 
Sbjct: 183 GGTITGAGKYLKEQNANVKLYGVEPVESAILSGGKPGPHKIQGIGAGFIPSVLNVDLIDE 242

Query: 287 VLEVKSDDAVKMARQLALQEGLLVGISSGANTIAALDLAKRPENKGKLIVTIHPSFGERY 346
           V++V SD+++ MARQLAL+EGLLVGISSGA   AA+ LA+RPEN GKL V I PSFGERY
Sbjct: 243 VVQVSSDESIDMARQLALKEGLLVGISSGAAAAAAIKLAQRPENAGKLFVAIFPSFGERY 302

Query: 347 LSSALFKELREEAENM 363
           LS+ LF   R+EAE M
Sbjct: 303 LSTVLFDATRKEAEAM 318

[Close]

BLAST of Spo01586.1 vs. TAIR (Arabidopsis)
Match: AT3G04940.1 (cysteine synthase D1)

HSP 1 Score: 374.8 bits (961), Expect = 6.200e-104
Identity = 183/315 (58.10%), Postives = 241/315 (76.51%), Query Frame = 1


		  
Query: 48  NIKTNVSQLIGRTPLVYLSKISEGSGAYIAVKQEMMQPTASVKDRPALAMIEDAEKKGLI 107
           +IK + +QLIG TP+VYL+ I +G  A IA K EMM+P +SVK+R A  MI+DAE KGLI
Sbjct: 7   SIKDDATQLIGNTPMVYLNNIVDGCVARIAAKLEMMEPCSSVKERIAYGMIKDAEDKGLI 66

Query: 108 SPGKTVLIEPTSGNMGISMAFMAAMKGYKMVLTMPSYTSMERRVVMRAFGADLILTDPDK 167
           +PGK+ LIE TSGN GI +AF+ A KGYK+VLTMPS  S+ER++++ A GA++ LTDP K
Sbjct: 67  TPGKSTLIEATSGNTGIGLAFIGAAKGYKVVLTMPSSMSLERKIILLALGAEVHLTDPSK 126

Query: 168 GMGGTVKKANQLLDSTPDGFMLQQFNNPANTQVHFETTGPEIWEDTQGKVDIFVMGIGSG 227
           G+ G + KA ++    PD  ML+QF NP+N Q H+ TTGPEIW D+ G+VDI V G+G+G
Sbjct: 127 GVQGIIDKAEEICSKNPDSIMLEQFKNPSNPQTHYRTTGPEIWRDSAGEVDILVAGVGTG 186

Query: 228 GTVSGVGRYLKSQNPNVKIYGVEPAESNILNGGKPGPHLITGNGVGFKPEILDMDVMDAV 287
           GT+SG GR+LK +N + K+YGVEP ES +++GGKPG HLI G G G  P+ LD +V+D V
Sbjct: 187 GTLSGSGRFLKEKNKDFKVYGVEPTESAVISGGKPGTHLIQGIGAGLIPDNLDFNVLDEV 246

Query: 288 LEVKSDDAVKMARQLALQEGLLVGISSGANTIAALDLAKRPENKGKLIVTIHPSFGERYL 347
           ++V S +A++ A+ LAL+EGLLVGISSGA   AA+ +AKRPEN GKLIV I PS GERYL
Sbjct: 247 IQVTSVEAIETAKLLALKEGLLVGISSGAAAAAAIKVAKRPENAGKLIVVIFPSGGERYL 306

Query: 348 SSALFKELREEAENM 363
           S++LF+ +R EAEN+
Sbjct: 307 STSLFESVRHEAENL 321

The following BLAST results are available for this feature:

BLAST of Spo01586.1 vs. NCBI nr
BLAST of Spo01586.1 vs. UniProtKB/TrEMBL
BLAST of Spo01586.1 vs. ExPASy Swiss-Prot
BLAST of Spo01586.1 vs. TAIR (Arabidopsis)

BLAST of Spo01586.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|75282478\|sp\|Q43153.1\|CYSC_SPIOL	6.0e-202	100.	RecName: Full=Bifunctional L-3...	[more]
gi\|902197360\|gb\|KNA13398.1\|	2.4e-198	94.8	hypothetical protein SOVF_1173...	[more]
gi\|731311581\|ref\|XP_010670142.1\|	1.8e-177	88.0	PREDICTED: bifunctional L-3-cy...	[more]
gi\|747045159\|ref\|XP_011092647.1\|	3.8e-156	77.3	PREDICTED: bifunctional L-3-cy...	[more]
gi\|470142107\|ref\|XP_004306760.1\|	1.9e-155	77.1	PREDICTED: L-3-cyanoalanine sy...	[more]

BLAST of Spo01586.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9R1K5_SPIOL	1.7e-198	94.8	Cysteine synthase OS=Spinacia ...	[more]
A0A0J8D7W0_BETVU	1.2e-177	88.0	Cysteine synthase OS=Beta vulg...	[more]
A0A166CYY9_DAUCA	3.9e-155	83.8	Uncharacterized protein OS=Dau...	[more]
M5X090_PRUPE	5.0e-155	76.8	Cysteine synthase OS=Prunus pe...	[more]
A5AEP0_VITVI	8.6e-155	78.4	Cysteine synthase OS=Vitis vin...	[more]

BLAST of Spo01586.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
CYSC_SPIOL	3.8e-204	100.	Bifunctional L-3-cyanoalanine ...	[more]
CAS1_SOLTU	2.7e-154	78.8	Bifunctional L-3-cyanoalanine ...	[more]
CAS1_MALDO	1.0e-153	74.0	L-3-cyanoalanine synthase 1, m...	[more]
CAS2_MALDO	1.4e-153	75.5	L-3-cyanoalanine synthase 2, m...	[more]
CAS2_SOLTU	6.3e-151	74.4	Bifunctional L-3-cyanoalanine ...	[more]

BLAST of Spo01586.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT3G61440.1	1.7e-149	74.3	cysteine synthase C1	[more]
AT3G59760.1	2.6e-118	62.3	O-acetylserine (thiol) lyase i...	[more]
AT2G43750.1	2.3e-114	60.1	O-acetylserine (thiol) lyase B	[more]
AT4G14880.1	1.9e-113	61.7	O-acetylserine (thiol) lyase (...	[more]
AT3G04940.1	6.2e-104	58.1	cysteine synthase D1	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001216	Cysteine synthase/cystathionine beta-synthase, pyridoxal-phosphate attachment site	PROSITE	PS00901	CYS_SYNTHASE	coord: 79..97 scor
IPR001926	Tryptophan synthase beta subunit-like PLP-dependent enzyme	PFAM	PF00291	PALP	coord: 54..340 score: 3.7
IPR001926	Tryptophan synthase beta subunit-like PLP-dependent enzyme	unknown	SSF53686	Tryptophan synthase beta subunit-like PLP-dependent enzymes	coord: 49..357 score: 1.7
IPR005856	Cysteine synthase	TIGRFAMs	TIGR01136	TIGR01136	coord: 54..352 score: 1.5E
IPR005859	Cysteine synthase CysK	TIGRFAMs	TIGR01139	TIGR01139	coord: 54..352 score: 2.0E
None	No IPR available	GENE3D	3.40.50.1100		coord: 183..354 score: 9.3E-77coord: 84..182 score: 4.1
None	No IPR available	PANTHER	PTHR10314	SER/THR DEHYDRATASE, TRP SYNTHASE	coord: 11..368 score: 2.5E

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019500	cyanide catabolic process
biological_process	GO:0032880	regulation of protein localization
biological_process	GO:0006833	water transport
biological_process	GO:0080147	root hair cell development
biological_process	GO:0009266	response to temperature stimulus
biological_process	GO:0009651	response to salt stress
biological_process	GO:0006535	cysteine biosynthetic process from serine
biological_process	GO:0046686	response to cadmium ion
biological_process	GO:0009750	response to fructose
biological_process	GO:0019288	isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
biological_process	GO:0009684	indoleacetic acid biosynthetic process
biological_process	GO:0006972	hyperosmotic response
biological_process	GO:0042744	hydrogen peroxide catabolic process
biological_process	GO:0007030	Golgi organization
biological_process	GO:0006096	glycolytic process
biological_process	GO:0019761	glucosinolate biosynthetic process
biological_process	GO:0051410	detoxification of nitrogen compound
biological_process	GO:0019499	cyanide metabolic process
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0009333	cysteine synthase complex
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0005507	copper ion binding
molecular_function	GO:0004124	cysteine synthase activity
molecular_function	GO:0050017	L-3-cyanoalanine synthase activity
molecular_function	GO:0016740	transferase activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo02068	0.65	Barchart \| Table
Spo14535	0.65	Barchart \| Table