Spo02313 (gene) | SpinachBase

Overview


Name	Spo02313
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Proteinase inhibitor
Location	chr5 : 2240560 .. 2244211 (-)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TAAGTTGCTCACAATTAACATTTTTAATTCAAAGTTTGACAATAATGGCTGCTAACGCTTCCTCAGCTCAAGTATAAATACACCACCATGCCACTTTAAACCCATAACTTAATTAAGCTTAGAATTAAGCATATTCATTAATTCATCTAATTAAGAAAAAGAAATATTTCGAAGAAGAAACAAAAGGGTAAAATAAAGAGAAAGATGTCGTCCGATTGCCCAGGTAATTAATCAATAGAGTTTTTATATATATGCTTCATTTTGTGTTTACTATAACTAATAATATAACTCGTATCATTTTACTATATTTCCTATGTATTACGTACTATGTACGTATATATTACCATGTACACGAGTATATTGTTGATGAAATAATTAAATGTAATCTATTACATTACATTATACTAAAACAGACATCAGAAATGACACATGTCACTTCCTAGTGCAAAGTTCTCCCGCCAAAAATCTCTTTCCTTAAAAAACAAATCTACTTCATTCTTTTTTATTTTCTTTCCTTTTATATAAATTTTTATATATGGAAAAATTTATAAGATTATAGAATATGATATTATTAGAGAATTTTTGTAATATTTTAGTCAAATCTTCATATCAATAATAGAAAATATTTTTACTCAATATTTTGGTCAAATTAACCTATTAACCATATCAAATATTTTGGTCAAATCATCATATTAAGCATGTAAAATATATAATATTTAATAGGACGGGCATTGTATATAATATGATAAAATGAGTACTTTCGAGATTTATTAATTACGCAATACATTAAAGGGATAAATATTTCATAAAAAAGATGGTTAAATATCCGTGCGTGCACAGAATCTAATCTAGTGATGTTATTATAGGGAAGAATGAGTGGCCAGAACTCGTGGGACTAAATGGAGAAGTGGCTGCACAGATAATAGAAAGTGAGAATCCCAACGTGCATGCCATCGTTTTAGAAGATGGATCTCCGACTACTAGAGACTTTGATTGCGATCGGGTTTGGGTTTGGGTCGATACTGACGGAGTTGTACGTGGTTCGTGCTCCTACAGCGGGTTAAGCGCGCTTTCAAGGTTACTATGTATTCTTCGTATGATAAACGTACTACCAAAAATGTATTTGGGTCTATGTAGTGATCCAACCTTAATTATATTTATGTTTTCAATCATGTACTGACCAATAAATATTGTACTACGGAGTACATAGTAAATAATATGGCCTTTCGGGTTTCTAGCTACTTCAATCATATACGGATATGTACAAAAAAAAAAAACTTATTGATGATAATAAAGTTTGTTTTCTACGTAGTACTACGTGTTTTTTAATTTAGGGCCTTCCACATAGTTTGTTAATTACTATTTACACGGACTCCAATGTAATATTTAACTATTAATATATCCAATTTCGTATGTGAAAAAAGTATAAAATATTGATATTATGAAAATACATACCGAGACCAATCTAACAAGATCTTACATGTAACGTTTTGATGTATATATTAATGATAATTTATGGTCAACGTTTTCATACTTTGGACACATATTCCAAAGCGTAAAGAACTTTCAGAAATTGAAGTAGTAATTGTTACATTATTTTGTTTTTAAGTTGATACAACTAGTACGAATCCACCTAGCTAGCTGGTTAATAGAGAATAGAATTCTCCTCGTATGAATTTGGGTTTGAGTTGATGATAACAAAGACGTGATTGGTCCTCTAACGAGTGGTTAATTACGCTATTCATCAAATAATATACAAGATTCATTTACAAGAGCAAACATGCTTAGTTATGTTTTATTGCATCCGATGAGAATCAATTTCTTTTTATGCGAATAAGCCATAAAGACAACATAAGTTATAAAGTAAAGGGGGTAGGAGATTTGAAATGAGACATGTCATATGGTACTAGCTAGAGCTGATCAATCTGAGTTAAGCCCGTGAATTAGCGGGGTTTGGACAAACTTTAGGCCTAGTCCGGCCCAGCCCAAATTTAAAAATTGTTACGGATTTTGGGCAATGTAAAACTACACTTTTAGTTATACATTTGGACCGGCCCGAGAATGGTCCGAAAGCCCATTATTTTTGTATGAAATAGCTAGCGGGATCTGGACACACAAAATCAGCCCGCATCTTGGCTAGGCCCTGCCCAACCCGACATAATGGACTAATTTTTAAGGCCGTGACGCGAACTTGCCCCGGCCCGCCATTTTATCAGTCGTACACAGGTCATCAATCTTCACTCTTAAGCCAAGACATGAGAATCATGACCGCATACTAACCAAACGCTTAATGAAGTTCTAAAACTAGTTGGCACACCATAATAGGTTAGGTAGCCAACCAAACTTATATACATACTCGTAGTAGTATGTTAATTAGTACTTCTTTTTTCGAAAGATAAGAAAAATAGTACATTAGATCCGTTCGATTTCTCATAAGTAATCACTGTCTAAACTTGAAACTAAAAGATTCAATACTACGAAGTATTATATAGCCAAATCTTTTTCTTCGAGTATTTTTTTTGATATTGGATAATTAGTCTCAATGGATCTATCATCTCGTGATAACTTTGTAATACTACTCGTATTGGTAATTTTTAATTTCCTACAAAATTTTAACCCACGTGGCCACATACTTAAATGTGATTCTTATCCTCTAATCTACTGACCACCTTCAAATCTTCAATTCATTGAACATCTACAATTATTTCCAATTTCAAATATGATCAACTTATTAAAACAACCAACTTAATTAATTTATCCATGTAATTGCATTATTATAAAATATAAATATGTGCCAACCATGCATACGCGTACAGATCGATCCAAAATCACAAGCTTTCATCACCCTATTTTTTATTTTATTTTTAAAAAAAAGTGGTCAATTTGTTAAATTTACGATATACTGTAACACGTATGTTAGTGTGTACATGCATGGCGGCCAAGAATATCACTTCAATTACTCCACGCGCCTTTTGCATTAATATAATATGCTCCCTCGACTATATATATATATATACATTGCCCTAGAAATATAGATATTAACTAGCTTAATTACTTATCTTATTATAAAGATCTGAGCATAAATCTAGTTTCAAAAGAAGGAGAAAGAACAAGAAAATAAAATGTCGACTGGAGATTTTTGCAAAGGTAGTGTTATGTATATCTTTGTTTTTCTAAGTTCTATTCACTTTTTACATCCGACATTTTTTTCTCCAATTATGGTCACCTGTACTTGATTTCACTTATGTCAACCTTTTATCCGATGACATATATTTTATCATCTTTCACAACTTAATTATATTGAGCAATATTATGTTACAGGGAAGAACGTGTGGCCCGAGCTAGTAGGAGAAAACGGGAAGGAAGCCGCAATAATCATAGAAAGAGAGAATCCTAATGTGGAAGCCATCGTCTTACCGGATGGTACCGCGGTTACAGATGATTTTAGGTGTGATCGGGTTTGGGTTTGGGTTGATTGTAATCAGTTGGTAGTTCGTCCTCCTACCATTGGTTAATTAAGAAATAAGTGCAATTTTTTTTCTATTGATTCAAAGAATATATAAAGTACGTACTCCGTACTAATTATGTACTTCGTTTATTTCGTATGAATAGCTAATTACATAAATAATAATGCCGTTCCAAGGCTTTCAAT

mRNA sequence

TAAGTTGCTCACAATTAACATTTTTAATTCAAAGTTTGACAATAATGGCTGCTAACGCTTCCTCAGCTCAAGTATAAATACACCACCATGCCACTTTAAACCCATAACTTAATTAAGCTTAGAATTAAGCATATTCATTAATTCATCTAATTAAGAAAAAGAAATATTTCGAAGAAGAAACAAAAGGGTAAAATAAAGAGAAAGATGTCGTCCGATTGCCCAGGGAAGAATGAGTGGCCAGAACTCGTGGGACTAAATGGAGAAGTGGCTGCACAGATAATAGAAAGTGAGAATCCCAACGTGCATGCCATCGTTTTAGAAGATGGATCTCCGACTACTAGAGACTTTGATTGCGATCGGGTTTGGGTTTGGGTCGATACTGACGGAGTTTTTCAAAAGAAGGAGAAAGAACAAGAAAATAAAATGTCGACTGGAGATTTTTGCAAAGGGAAGAACGTGTGGCCCGAGCTAGTAGGAGAAAACGGGAAGGAAGCCGCAATAATCATAGAAAGAGAGAATCCTAATGTGGAAGCCATCGTCTTACCGGATGGTACCGCGGTTACAGATGATTTTAGGTGTGATCGGGTTTGGGTTTGGGTTGATTGTAATCAGTTGGTAGTTCGTCCTCCTACCATTGGTTAATTAAGAAATAAGTGCAATTTTTTTTCTATTGATTCAAAGAATATATAAAGTACGTACTCCGTACTAATTATGTACTTCGTTTATTTCGTATGAATAGCTAATTACATAAATAATAATGCCGTTCCAAGGCTTTCAAT

Coding sequence (CDS)

ATGTCGTCCGATTGCCCAGGGAAGAATGAGTGGCCAGAACTCGTGGGACTAAATGGAGAAGTGGCTGCACAGATAATAGAAAGTGAGAATCCCAACGTGCATGCCATCGTTTTAGAAGATGGATCTCCGACTACTAGAGACTTTGATTGCGATCGGGTTTGGGTTTGGGTCGATACTGACGGAGTTTTTCAAAAGAAGGAGAAAGAACAAGAAAATAAAATGTCGACTGGAGATTTTTGCAAAGGGAAGAACGTGTGGCCCGAGCTAGTAGGAGAAAACGGGAAGGAAGCCGCAATAATCATAGAAAGAGAGAATCCTAATGTGGAAGCCATCGTCTTACCGGATGGTACCGCGGTTACAGATGATTTTAGGTGTGATCGGGTTTGGGTTTGGGTTGATTGTAATCAGTTGGTAGTTCGTCCTCCTACCATTGGTTAA

Protein sequence

MSSDCPGKNEWPELVGLNGEVAAQIIESENPNVHAIVLEDGSPTTRDFDCDRVWVWVDTDGVFQKKEKEQENKMSTGDFCKGKNVWPELVGENGKEAAIIIERENPNVEAIVLPDGTAVTDDFRCDRVWVWVDCNQLVVRPPTIG

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo02313.1	Spo02313.1	mRNA

Homology

[Close]

BLAST of Spo02313.1 vs. NCBI nr
Match: gi|902228140|gb|KNA20989.1| (hypothetical protein SOVF_047400 [Spinacia oleracea])

HSP 1 Score: 158.3 bits (399), Expect = 1.000e-35
Identity = 71/72 (98.61%), Postives = 72/72 (100.00%), Query Frame = 1


		  
Query: 74  MSTGDFCKGKNVWPELVGENGKEAAIIIERENPNVEAIVLPDGTAVTDDFRCDRVWVWVD 133
           MSTGDFCKGKNVWPELVGENGKEAAIIIERENPNVEAIVLPDGTAVTDDFRCDRVWVWVD
Sbjct: 1   MSTGDFCKGKNVWPELVGENGKEAAIIIERENPNVEAIVLPDGTAVTDDFRCDRVWVWVD 60

Query: 134 CNQLVVRPPTIG 146
           CN+LVVRPPTIG
Sbjct: 61  CNRLVVRPPTIG 72

[Close]

BLAST of Spo02313.1 vs. NCBI nr
Match: gi|703112260|ref|XP_010100075.1| (Proteinase inhibitor [Morus notabilis])

HSP 1 Score: 151.4 bits (381), Expect = 1.200e-33
Identity = 74/142 (52.11%), Postives = 95/142 (66.90%), Query Frame = 1


		  
Query: 1   MSSDCPGKNEWPELVGLNGEVAAQIIESENPNVHAIVLEDGSPTTRDFDCDRVWVWVDTD 60
           M+S+C GK+ WPEL+G NGE AA+ IE EN NV A V+ +G+  T+DF CDRVWVWV+  
Sbjct: 1   MASECQGKSSWPELLGKNGEEAAKQIEKENKNVDAKVILEGTAVTKDFRCDRVWVWVNNY 60

Query: 61  GVFQKKEKEQENKMSTGDFCKGKNVWPELVGENGKEAAIIIERENPNVEAIVLPDGTAVT 120
           G   +  +             GKN WPELVG NGKEA   IE+EN NV+AIV+ +GT V 
Sbjct: 61  GTVTEVPR------------VGKNSWPELVGVNGKEAEKQIEKENDNVDAIVVLEGTPVP 120

Query: 121 DDFRCDRVWVWVDCNQLVVRPP 143
           +DFRC RVWVWV+ + +V + P
Sbjct: 121 EDFRCTRVWVWVNDSGIVTQLP 130

[Close]

BLAST of Spo02313.1 vs. NCBI nr
Match: gi|902228141|gb|KNA20990.1| (hypothetical protein SOVF_047410 [Spinacia oleracea])

HSP 1 Score: 137.9 bits (346), Expect = 1.400e-29
Identity = 62/62 (100.00%), Postives = 62/62 (100.00%), Query Frame = 1


		  
Query: 1  MSSDCPGKNEWPELVGLNGEVAAQIIESENPNVHAIVLEDGSPTTRDFDCDRVWVWVDTD 60
          MSSDCPGKNEWPELVGLNGEVAAQIIESENPNVHAIVLEDGSPTTRDFDCDRVWVWVDTD
Sbjct: 1  MSSDCPGKNEWPELVGLNGEVAAQIIESENPNVHAIVLEDGSPTTRDFDCDRVWVWVDTD 60

Query: 61 GV 63
          GV
Sbjct: 61 GV 62

[Close]

BLAST of Spo02313.1 vs. NCBI nr
Match: gi|645260368|ref|XP_008235797.1| (PREDICTED: proteinase inhibitor-like [Prunus mume])

HSP 1 Score: 129.8 bits (325), Expect = 3.900e-27
Identity = 68/143 (47.55%), Postives = 88/143 (61.54%), Query Frame = 1


		  
Query: 3   SDCPGKNEWPELVGLNGEVAAQIIESENPNVHAIVLEDGSPTTRDFDCDRVWVWVDTDGV 62
           S CP K  WPE+VG +GE AA  IE EN NV AIV+  GSPTTRD               
Sbjct: 2   SHCPSKKSWPEVVGQSGEDAAARIERENHNVIAIVILKGSPTTRDM-------------- 61

Query: 63  FQKKEKEQENKMSTGDFCKGKNVWPELVGENGKEAAIIIERENPNVEAIVLPDGTAVTDD 122
             ++++ +   +  G    GK  WPE+VG++G++AA  IEREN NV AIV+P+G+A T D
Sbjct: 62  --RRKRARTMPLCPG---AGKKSWPEVVGQSGEDAAAKIERENHNVRAIVIPEGSATTLD 121

Query: 123 FRCDRVWVWVDCNQLVVRPPTIG 146
            RCDRVWVWV+ N  V + P +G
Sbjct: 122 RRCDRVWVWVNRNGTVTKAPKVG 125

[Close]

BLAST of Spo02313.1 vs. NCBI nr
Match: gi|870858830|gb|KMT10318.1| (hypothetical protein BVRB_5g120690 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 129.0 bits (323), Expect = 6.600e-27
Identity = 57/72 (79.17%), Postives = 63/72 (87.50%), Query Frame = 1


		  
Query: 74  MSTGDFCKGKNVWPELVGENGKEAAIIIERENPNVEAIVLPDGTAVTDDFRCDRVWVWVD 133
           MS  DFCKGKN WPELVGENGK AA IIE EN NV+AIVLPDGTAV +DFRC RVWVWVD
Sbjct: 1   MSGEDFCKGKNAWPELVGENGKIAAQIIESENDNVKAIVLPDGTAVPEDFRCTRVWVWVD 60

Query: 134 CNQLVVRPPTIG 146
           C+++V+RPPTIG
Sbjct: 61  CDEIVIRPPTIG 72

[Close]

BLAST of Spo02313.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RQ05_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_047400 PE=4 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 7.100e-36
Identity = 71/72 (98.61%), Postives = 72/72 (100.00%), Query Frame = 1


		  
Query: 74  MSTGDFCKGKNVWPELVGENGKEAAIIIERENPNVEAIVLPDGTAVTDDFRCDRVWVWVD 133
           MSTGDFCKGKNVWPELVGENGKEAAIIIERENPNVEAIVLPDGTAVTDDFRCDRVWVWVD
Sbjct: 1   MSTGDFCKGKNVWPELVGENGKEAAIIIERENPNVEAIVLPDGTAVTDDFRCDRVWVWVD 60

Query: 134 CNQLVVRPPTIG 146
           CN+LVVRPPTIG
Sbjct: 61  CNRLVVRPPTIG 72

[Close]

BLAST of Spo02313.1 vs. UniProtKB/TrEMBL
Match: W9RJZ5_9ROSA (Proteinase inhibitor OS=Morus notabilis GN=L484_005749 PE=4 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 8.600e-34
Identity = 74/142 (52.11%), Postives = 95/142 (66.90%), Query Frame = 1


		  
Query: 1   MSSDCPGKNEWPELVGLNGEVAAQIIESENPNVHAIVLEDGSPTTRDFDCDRVWVWVDTD 60
           M+S+C GK+ WPEL+G NGE AA+ IE EN NV A V+ +G+  T+DF CDRVWVWV+  
Sbjct: 1   MASECQGKSSWPELLGKNGEEAAKQIEKENKNVDAKVILEGTAVTKDFRCDRVWVWVNNY 60

Query: 61  GVFQKKEKEQENKMSTGDFCKGKNVWPELVGENGKEAAIIIERENPNVEAIVLPDGTAVT 120
           G   +  +             GKN WPELVG NGKEA   IE+EN NV+AIV+ +GT V 
Sbjct: 61  GTVTEVPR------------VGKNSWPELVGVNGKEAEKQIEKENDNVDAIVVLEGTPVP 120

Query: 121 DDFRCDRVWVWVDCNQLVVRPP 143
           +DFRC RVWVWV+ + +V + P
Sbjct: 121 EDFRCTRVWVWVNDSGIVTQLP 130

[Close]

BLAST of Spo02313.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RN96_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_047410 PE=4 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 9.900e-30
Identity = 62/62 (100.00%), Postives = 62/62 (100.00%), Query Frame = 1


		  
Query: 1  MSSDCPGKNEWPELVGLNGEVAAQIIESENPNVHAIVLEDGSPTTRDFDCDRVWVWVDTD 60
          MSSDCPGKNEWPELVGLNGEVAAQIIESENPNVHAIVLEDGSPTTRDFDCDRVWVWVDTD
Sbjct: 1  MSSDCPGKNEWPELVGLNGEVAAQIIESENPNVHAIVLEDGSPTTRDFDCDRVWVWVDTD 60

Query: 61 GV 63
          GV
Sbjct: 61 GV 62

[Close]

BLAST of Spo02313.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CE78_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_5g120690 PE=4 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 4.600e-27
Identity = 57/72 (79.17%), Postives = 63/72 (87.50%), Query Frame = 1


		  
Query: 74  MSTGDFCKGKNVWPELVGENGKEAAIIIERENPNVEAIVLPDGTAVTDDFRCDRVWVWVD 133
           MS  DFCKGKN WPELVGENGK AA IIE EN NV+AIVLPDGTAV +DFRC RVWVWVD
Sbjct: 1   MSGEDFCKGKNAWPELVGENGKIAAQIIESENDNVKAIVLPDGTAVPEDFRCTRVWVWVD 60

Query: 134 CNQLVVRPPTIG 146
           C+++V+RPPTIG
Sbjct: 61  CDEIVIRPPTIG 72

[Close]

BLAST of Spo02313.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BS38_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_8g194020 PE=4 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 1.700e-21
Identity = 50/67 (74.63%), Postives = 55/67 (82.09%), Query Frame = 1


		  
Query: 80  CKGKNVWPELVGENGKEAAIIIERENPNVEAI-VLPDGTAVTDDFRCDRVWVWVDCNQLV 139
           C GKNVWPELVGE GK AA +I+RENPNV  + VLPDGT V  +FRCDRVWVWVDC +LV
Sbjct: 5   CPGKNVWPELVGEKGKVAAEVIQRENPNVTQVTVLPDGTPVILNFRCDRVWVWVDCEELV 64

Query: 140 VRPPTIG 146
           VRPP IG
Sbjct: 65  VRPPMIG 71

[Close]

BLAST of Spo02313.1 vs. ExPASy Swiss-Prot
Match: ITH5_CUCMA (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima PE=1 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 6.200e-17
Identity = 39/66 (59.09%), Postives = 48/66 (72.73%), Query Frame = 1


		  
Query: 80  CKGKNVWPELVGENGKEAAIIIERENPNVEAIVLPDGTAVTDDFRCDRVWVWVDCNQLVV 139
           C GK+ WP LVG  G  A  IIER+NPNV+A++L +GT VT DFRC+RV +WV+   LVV
Sbjct: 3   CPGKSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVNKRGLVV 62

Query: 140 RPPTIG 146
            PP IG
Sbjct: 63  SPPRIG 68

[Close]

BLAST of Spo02313.1 vs. ExPASy Swiss-Prot
Match: ICI_LINUS (Proteinase inhibitor OS=Linum usitatissimum PE=1 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 4.000e-16
Identity = 39/61 (63.93%), Postives = 46/61 (75.41%), Query Frame = 1


		  
Query: 2  SSDCPGKNEWPELVGLNGEVAAQIIESENPNVHAIVLEDGSPTTRDFDCDRVWVWVDTDG 61
          S  CPGKN WPELVG +G +AA  +E EN NVHAIVL++GS  T+DF CDRVWV V+  G
Sbjct: 1  SRRCPGKNAWPELVGKSGNMAAATVERENRNVHAIVLKEGSAMTKDFRCDRVWVIVNDHG 60

Query: 62 V 63
          V
Sbjct: 61 V 61

[Close]

BLAST of Spo02313.1 vs. ExPASy Swiss-Prot
Match: ATSI_AMACA (Trypsin/subtilisin inhibitor OS=Amaranthus caudatus PE=1 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 4.000e-16
Identity = 40/65 (61.54%), Postives = 46/65 (70.77%), Query Frame = 1


		  
Query: 80  CKGKNVWPELVGENGKEAAIIIERENPNVEAIVLPDGTAVTDDFRCDRVWVWVDCNQLVV 139
           C GK  WPELVGE G +AA IIERENPNV +IV  + +  T DFRCDRVWV VD   +VV
Sbjct: 4   CPGKQEWPELVGEYGYKAAAIIERENPNVRSIVKHERSGFTKDFRCDRVWVVVDSTGVVV 63

Query: 140 RPPTI 145
           R P +
Sbjct: 64  RTPRV 68

[Close]

BLAST of Spo02313.1 vs. ExPASy Swiss-Prot
Match: BGIA_MOMCH (Glu S.griseus protease inhibitor OS=Momordica charantia PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 8.900e-16
Identity = 37/66 (56.06%), Postives = 45/66 (68.18%), Query Frame = 1


		  
Query: 80  CKGKNVWPELVGENGKEAAIIIERENPNVEAIVLPDGTAVTDDFRCDRVWVWVDCNQLVV 139
           C+GK  WP+LVG  G  A  +IERENP V A+++  G+ VT DFRCDRV VWV    +V 
Sbjct: 3   CQGKRSWPQLVGSTGAAAKAVIERENPRVRAVIVRVGSPVTADFRCDRVRVWVTERGIVA 62

Query: 140 RPPTIG 146
           RPP IG
Sbjct: 63  RPPAIG 68

[Close]

BLAST of Spo02313.1 vs. ExPASy Swiss-Prot
Match: HPI_HEVBR (Protease inhibitor HPI OS=Hevea brasiliensis GN=PI1 PE=1 SV=2)

HSP 1 Score: 76.6 bits (187), Expect = 2.400e-13
Identity = 35/62 (56.45%), Postives = 48/62 (77.42%), Query Frame = 1


		  
Query: 1  MSSDCPGKNEWPELVGLNGEVAAQIIESENPNVHAIVLEDGSPTTRDFDCDRVWVWVDTD 60
          M+S CP KN WPELVG NG++AA II++EN NV AIV+++G P T+D + +RV V+VD +
Sbjct: 1  MASQCPVKNSWPELVGTNGDIAAGIIQTENANVKAIVVKEGLPITQDLNFNRVRVFVDEN 60

Query: 61 GV 63
           V
Sbjct: 61 RV 62

[Close]

BLAST of Spo02313.1 vs. TAIR (Arabidopsis)
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein)

HSP 1 Score: 87.4 bits (215), Expect = 7.700e-18
Identity = 40/66 (60.61%), Postives = 48/66 (72.73%), Query Frame = 1


		  
Query: 80  CKGKNVWPELVGENGKEAAIIIERENPNVEAIVLPDGTAVTDDFRCDRVWVWVDCNQLVV 139
           C  KN WPEL G NG  AA++IERENP V A V+ DG+ VT DFRCDRV V+VD N++VV
Sbjct: 5   CPRKNSWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDGNRIVV 64

Query: 140 RPPTIG 146
           + P  G
Sbjct: 65  KTPKSG 70

[Close]

BLAST of Spo02313.1 vs. TAIR (Arabidopsis)
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein)

HSP 1 Score: 74.7 bits (182), Expect = 5.200e-14
Identity = 32/64 (50.00%), Postives = 47/64 (73.44%), Query Frame = 1


		  
Query: 82  GKNVWPELVGENGKEAAIIIERENPNVEAIVLPDGTAVTDDFRCDRVWVWVDCNQLVVRP 141
           GK+ WPEL+G  G++A  +IERENP ++A+++ DGT V + F C RV+VWV+   +VV+ 
Sbjct: 36  GKSSWPELLGAKGEDAKEVIERENPKMKAVIILDGTVVPEIFICSRVYVWVNDCGIVVQI 95

Query: 142 PTIG 146
           P IG
Sbjct: 96  PIIG 99

[Close]

BLAST of Spo02313.1 vs. TAIR (Arabidopsis)
Match: AT2G38900.2 (Serine protease inhibitor, potato inhibitor I-type family protein)

HSP 1 Score: 74.3 bits (181), Expect = 6.800e-14
Identity = 34/64 (53.12%), Postives = 44/64 (68.75%), Query Frame = 1


		  
Query: 82  GKNVWPELVGENGKEAAIIIERENPNVEAIVLPDGTAVTDDFRCDRVWVWVDCNQLVVRP 141
           GKN WPEL+G NG  AA +I+ EN ++  +V+ DG  VT+D  C RV VWVD  ++VVR 
Sbjct: 25  GKNSWPELLGTNGDYAASVIKGENSSLNVVVVSDGNYVTEDLSCYRVRVWVDEIRIVVRN 84

Query: 142 PTIG 146
           PT G
Sbjct: 85  PTAG 88

[Close]

BLAST of Spo02313.1 vs. TAIR (Arabidopsis)
Match: AT3G46860.1 (Serine protease inhibitor, potato inhibitor I-type family protein)

HSP 1 Score: 65.1 bits (157), Expect = 4.100e-11
Identity = 32/60 (53.33%), Postives = 41/60 (68.33%), Query Frame = 1


		  
Query: 86  WPELVGENGKEAAIIIERENPNVEAIVLPDGTAVTDDFRCDRVWVWVDCNQLVVRPPTIG 145
           WPELVG+NG+ A + IERENPNV AIVL  G    ++F C+RV+V++  N  V   P IG
Sbjct: 26  WPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIENFCCNRVFVYLGSNGQVADAPMIG 85

[Close]

BLAST of Spo02313.1 vs. TAIR (Arabidopsis)
Match: AT5G43570.1 (Serine protease inhibitor, potato inhibitor I-type family protein)

HSP 1 Score: 53.5 bits (127), Expect = 1.200e-7
Identity = 26/60 (43.33%), Postives = 35/60 (58.33%), Query Frame = 1


		  
Query: 83  KNVWPELVGENGKEAAIIIERENPNVEAIVLPDGTAVTDDFRCDRVWVWVDCNQLVVRPP 142
           K+ WPELVG  G+E   II+REN  V A ++ +   V     CDRV+V V+   +V R P
Sbjct: 14  KSSWPELVGRRGEEVKEIIDRENTKVTAKIISENAVVLAVVICDRVYVRVNDQGIVTRTP 73

The following BLAST results are available for this feature:

BLAST of Spo02313.1 vs. NCBI nr
BLAST of Spo02313.1 vs. UniProtKB/TrEMBL
BLAST of Spo02313.1 vs. ExPASy Swiss-Prot
BLAST of Spo02313.1 vs. TAIR (Arabidopsis)

BLAST of Spo02313.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902228140\|gb\|KNA20989.1\|	1.0e-35	98.6	hypothetical protein SOVF_0474...	[more]
gi\|703112260\|ref\|XP_010100075.1\|	1.2e-33	52.1	Proteinase inhibitor [Morus no...	[more]
gi\|902228141\|gb\|KNA20990.1\|	1.4e-29	100.	hypothetical protein SOVF_0474...	[more]
gi\|645260368\|ref\|XP_008235797.1\|	3.9e-27	47.5	PREDICTED: proteinase inhibito...	[more]
gi\|870858830\|gb\|KMT10318.1\|	6.6e-27	79.1	hypothetical protein BVRB_5g12...	[more]

BLAST of Spo02313.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RQ05_SPIOL	7.1e-36	98.6	Uncharacterized protein OS=Spi...	[more]
W9RJZ5_9ROSA	8.6e-34	52.1	Proteinase inhibitor OS=Morus ...	[more]
A0A0K9RN96_SPIOL	9.9e-30	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8CE78_BETVU	4.6e-27	79.1	Uncharacterized protein OS=Bet...	[more]
A0A0J8BS38_BETVU	1.7e-21	74.6	Uncharacterized protein OS=Bet...	[more]

BLAST of Spo02313.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
ITH5_CUCMA	6.2e-17	59.0	Inhibitor of trypsin and hagem...	[more]
ICI_LINUS	4.0e-16	63.9	Proteinase inhibitor OS=Linum ...	[more]
ATSI_AMACA	4.0e-16	61.5	Trypsin/subtilisin inhibitor O...	[more]
BGIA_MOMCH	8.9e-16	56.0	Glu S.griseus protease inhibit...	[more]
HPI_HEVBR	2.4e-13	56.4	Protease inhibitor HPI OS=Heve...	[more]

BLAST of Spo02313.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT2G38870.1	7.7e-18	60.6	Serine protease inhibitor, pot...	[more]
AT5G43580.1	5.2e-14	50.0	Serine protease inhibitor, pot...	[more]
AT2G38900.2	6.8e-14	53.1	Serine protease inhibitor, pot...	[more]
AT3G46860.1	4.1e-11	53.3	Serine protease inhibitor, pot...	[more]
AT5G43570.1	1.2e-7	43.3	Serine protease inhibitor, pot...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRODOM	PD002604		coord: 11..61 score: 2.0E-13coord: 86..145 score: 2.0
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRINTS	PR00292	POTATOINHBTR	coord: 122..132 score: 4.7E-7coord: 110..121 score: 4.7E-7coord: 85..98 score: 4.
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 8..62 score: 1.4E-21coord: 83..145 score: 1.2
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PROSITE	PS00285	POTATO_INHIBITOR	coord: 86..97 score: -coord: 11..22 scor
IPR000864	Proteinase inhibitor I13, potato inhibitor I	unknown	SSF54654	CI-2 family of serine protease inhibitors	coord: 79..145 score: 8.9E-25coord: 4..62 score: 3.27
None	No IPR available	GENE3D	3.30.10.10		coord: 79..145 score: 1.4E-28coord: 4..62 score: 1.7
None	No IPR available	PANTHER	PTHR33091	FAMILY NOT NAMED	coord: 51..145 score: 2.1
None	No IPR available	PANTHER	PTHR33091:SF14	SUBFAMILY NOT NAMED	coord: 51..145 score: 2.1

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo21248	0.70	Barchart \| Table
Spo19335	0.65	Barchart \| Table