Spo03181 (gene) | SpinachBase

Overview


Name	Spo03181
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase family protein
Location	SpoScf_01703 : 28527 .. 55466 (+)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCACGAGCAGACTCGGTTCGTTAACGCACCTCTAAACTCCTCACGACTCCACCGCGCCACAGCTAAAAACAACAAACAGCGTCGTTAACTCGTTATCACTTCCACAGACTTCACTCACGCTGAGAAGCACAGCACACAAGATGATCGCCATTACCCAAATCCAACCATTAACAACACCACACTCACTCCAAAATGCATATCACCAAACCAAAAACCTCTCATTTTACTCTTCTCAAACTTCCGTCGTCAAATTGCGCATTCCTAACTTCTCAAACCCCAACAAACCACCTCTTTCCTCCAGAATCGCCGCCATGTCCGTCGCCGACCAAGCTAGGGTTCCTCTCGCACTTCCTTTGCCTCCTCCTCCGGTCACTAAGGTCTTTCTGTCGCTTTATCTCTCCTCTTCACCTATTTCATCTATGGTTTCGTATGCCAGCTCTGATCAATCGTTTGTGCAATCGCAATTTCGTTTTGTATTCGTAATTTCATGCGTTCTTCAATTGGTAAATTAGAGTTGACGATGCCGTCTATTTGGTGGAATTATCGTAATTGGATATAGTTTTTTCGCGAAATTTGTGAGGAAATTTTTGTTAAGTTCAGTTAGTCATAGTCAACTAGCTGTTAGTTGAATCCTACATAGTAGGATTAGTTGCTGAGAATAATGGCAGAATATTAGGAGTTTGTTTTGGAGTTTTAGTTTGGGTTTGTTACTAGTTTTGTGGTAGTTAGTTGTCAGCAAGTTCTATATAAGGAGCATGCTAGTGTACTGTTTTAGACGTATTGAGTGAGCAATTCAATTTCCCTTTTCTTCTCTATATTCTTCATCTTTAACAGATTAACAGTGGTATCAGAGCTTGGTTACTGAGAAGAGTGAGTTTGAGCGTCCACAAAAATTCTGAACAATTGTATTGCAGACTGTAGTAGCAGTCCTTTTTCATGGCATCTGAGAATTTCGTTCAGGCTGCAATCCCTCGGTTTGATGGTCATTATGATCATTGGAGCATGTTGATGGAGAATTTCCTAAGATCAAAGGAATTCTGGGCTGTAGTAGTCTCTGGCGTAGCAGAACCAGCCAATACAGAGCGGCTGACAGATGCACAAAAATCTCAATTTGAAGCCTTGAAGTTGAAGGATCTCAAGGCCAAGAACTATCTGTTTCAGGCTATAGACAGATCAATCTTGGAGACCATTCTTTGCAAAGATTCCTCCAAAGATATTTGGGATGCCATGAAAAGGAAGTACCAAGGAAACGCAAGATCAAAGCGGGTGATGTTGCAGGGACTTCGTGAGGATTTTGATGGTCTGCGTATGAAGAAAGGAGAATCAGTTGATGATTTCTTTTCAAGGATGATGACAATCGTCAACAAGATGCGGGTCCATGGAGAAAAGAAGGATGATGCCATGGTCGTAGACAAGATTCTTCGATCCCTCACAAAAGATTTCTAACTTTATTGTCTGCTCCATTGAGGAATCAAAGGATCTGGATGAACTCTCAATTGATGAATTACAGAATTCATTGCGAGTTCATGAGCGGAAGCTAGGCAGACAAGATGACGAGGAGCAGGCCTTACAAGTAACAACCAATATTAGTTCGTCATCTAATAATGACAATCAAAGTACAGGCAGAAGCTACAAGGGACGCAATAATCAAAGGAAATGGAGAGGTCGAGGAGGACATCAGTCATGGAGAAATAAATCAAGGTCAGTAGACAAGTCCAAAATTCAATGCCATAGATGTCATTACTTTGGACATTATAAGAATGAGTGTCGAACTAACTTGAATGGTCGAGGAGGCGAAAACTCCAATTATGTTGAAACTGAAGAGAAATCAGAAGAGATCTCTTTACTGATGGTGTCTCAAGAAAAGGAAGATACTCCAAAGAGCATGTGGTATCTAGATACTGGTTGCAGCAACCACATGTGTGGAGAAAGGAAGGCATTCTCCGAGTTAGATGAATCTTACCGTGATGCTGTCAAGTTTGGTGACAACACAAGCGTGAATGTCATGGGGAAAGGAAAGGTAACAGTCCAAACCGAAAGAAACTCAACCCACACTATTTCTAATGTTCTTTATGTGCCTGTTTTGAAGAAGAATTTGCTGAGTGTGGGTCAACTCCAAGAAAAGGGGTATGAGATTTGCATAAAGAATGGAGCATGTACGATACGAGATGAAAAGTTGGCCTTGATAGCCAACGTGAAGATGACTGCAAACAGAATGTTTCCACTCTACCTTCAGAATGTGAGTCACTTGTGTCTCTCAGCAAAATTGAAGGATAAAGTGTGGCTCTGGCACCTCCGGTATGGGCACTTAAGCTTTGGTGGTTTGAAGACATTGGTGCAGAAAGATATGGTGAGTGGCCTCCCTTCAATAACAATCCCATCTGAAGTGTGTGAAGACTGTGTAGTTGGCAAACAACATAGAGCCCCATTTCCGAAAGGAAAAGTTCGCAGGGCAACGAGAGTGCTTGAAGTGGTGCATTCTGATCTTTGTGGGCCAATAAATCCCACATCCAATGGAGGTAAACGATACTTTATTACCTTTATTGATGACTTAAGTAGGAAGGCATGGGTATATTTCTTGCAGGATAAATCTGAAGCCTTTACAGCTTTCAAAAATTACAAAGCATTGGTTGAGAAAGAAACAGGGAGTTATATCTCTGTGCTTCGCACTGATCGGGGAGGTGAATATAACTCACAGGAATTTAAGAATTTTTGTGAGGAGCATGGTGTCAAACGACAGCTCACAGCAGCCTACACACCTCAACAAAATGATGTATGTGAAAGGAAGAATCGCACGATAATGAATATGGTGCGTAGTCTCTTGGTGATGAGTGGAGTACCTAAGAGTTTCTGGCCCGAGGCAGTGAATTGGAGCATTCATGTTCTGAATAGAAGCCCCACTTTTTCTGTTCAGAACATGACTCCTGAAGAAGCTTGGAGCGGACGAAGGCCTGATGTTGATCACTTCAGAGTTTTCGGAAGCATAGCATATGCTCATGTTCCAGATCAAAGAAGAAAAAAGTTAGACCACAAAGGAGAAAAATGCATCTTTCTTGGTGTTAGTGGTCAATCGAAAGCCTACAAGTTATATAATCCTATCACTAAGAAGATGCTCACCAGTCGAGATGTTGTCTTTGATGAAGAAAACTTTTGGTCGTGGAAGAACACAGGTAATGTTAAGCAAATTCCAGTGCATCTTGACAGTGAAAAGGATGCTGCAACAATTCAACCCCAACAAGAAGTAGTTACTCCTACTGAAAATCAGCCTGCAAGTCCCATTATTATAGTTGCAACTCCCAGCAACGATGATCAGCAACAAGGAGCACCTGGAAGCGCTCGATTAAGAAGGCGGCCAAGATGGATGTCAGACTTTGAGGTACAACTACCTGGTCATGATGATTCAGATGATATCCTCACGCATTTTGCTTTGTTCTCAGATTGTGATCCCACAAGCTATGAAGATGCTGTCAGAGAACAAAAGTGGCAAAATGCTATGAATGAGGAAATAACATCCATTGAGAGAAATAATACATGGGAGCTGGTTGATCTTCCGGAAGGACAGAAGTCCATTGGTGTGAAATGGGTGTACAAGACAAAGCTGAAAGAGAATGGAGATGTGCAAAAATACAAGGCCCGTCTTGTAGCTAAAGGATACAAGCAAGAGTTAGGGATTGATTACACAGAAGTGTTTGCTCCAGTTGCTAGACATGATACAATTCGGTTGGTTGTTGCATTGGCAGCTTGTAACTCATGGCCCATCTTCCAGTTAGATGTGAAGTCAGCATTTTTGCATGGAGAACTACAAGAAGAGGTATTTATTGATCAACCTCCTGGTTATGTGAATCCTGGTAGTGAACACAAGGTTTACAAATTAAAAAAGGCCCTATACGGACTGAAACAAGCACCAAGAGCTTGGTACAGCCGTATAGATGCTTATTTTTTAAGGGAGGGATTTAAAAGATGCCCATATGAGCATACACTTTACACCAAAATTGACAATGGTGGAAAGATGATTATGGTATGCTTATATGTGGATGATTTGATTTATACTGGAAATGATAGAGCCATGTTTGAAAATTTTAAGATATCGATGATGAATGAATTTGATATGACTGATCTTGGGATGATGCATTATTTTCTTGGGATAGAAGTGGTTCAATCTGCCACTGAAATCTTCTTGTGTCAGAAGAAGTATCTGCTGGAGGTGCTGGACAGGTTTGGTATGACCAATAGTAACCCTGTCACAACTCCAACAGAACGGAGTATAAAGCTTGTGAAGGATCCTTCAGGAGACGAGGTTAATAGTACTTTGTACAAGCAGATAGTAGGAAGCTAGCTTGATGTACTTGACAGCAACACGTCCTGATATAATGCATGCTGTGAGTATGATTAGCAGGTACATGGAAAGCCCAAAAGAGATTCATCTCTTAGCTGCAAAGAGAATTCTTCGTTACCTACGGGGGACTACAGACTTTGGGCTTTTCTACAGCAAAGGTGGAAAATCAGAGTTGGTTGGATTTTGTGATAGTGATTATGCTGGAGACTTGGATGACAGAAGAAGTACTACTGGATATGCTTTCATGTTGGGTTCTGCAGCTGTTTCGTGGTGTTCAAAGAAGCAGCCTATAGTCACTTTATCAACCACTGAAGCGGAATTTGTAGCAGCAGCAACCTGTGCAAGTCAGGCACTTTGGTTAAGAAATCTTCTTGAAGAGCTTCAGTTCAAGCAGGAGTATTCTACTCAATTATTTTGTGACAACAGTTCAGCTATTAAGCTCTCAAGGAATCCAGTGTTTCATGGAAAATGCAAGCACATCGATGTTAGATATCACTTCTTACGTGATCTCTCTAAAGATGGAATCATTGATCTAGTTTTCTGCGGGAGTGCTGATCAAGTTGCTGATGTGTTCACAAAGTCTCTCAAACTGGACAGCTTTGTGAAGTTTAGAAGGCTACTTGGAGTCTGCACCATGAAGAATCCTGTTTGATAAAAGGCTTTATTTATGTAATTAAACTGATCCTTTGCAAAGTTCAGTTTAAGGGAGGGTATGTTAAGTTCAGTTAGTCATAGTCAACTAGCTGTTAGTTGAATCCTACATAGTAGGATTAGTTGCTGAGAATAATGGCAGAATATTAGGAGTTTGTTTTGGAGTTTTAGTTTGGGTTTGTTCCTAGTTTTGTGGTAGTTAGTTGTCAGGAAGTTCTATATAAGGAGCATGCTAGTGTACTGTTTTAGACGTATTGAGTGAGCAATTCAATTTCCCTTTTCTTCTCTATATTCTTCATCTTTAACAGATTAACAATTTTTGTTCAATGTTTTCAATTCATTCATTTTCTCCCGCACTTCCATTGGTTCTTCCTTCAATCACTAAGGTCTGTCTGTCATTTTCTCTCTCCTCTTCACCATTTCATCTATGGTTTCGAATGCCGGCTTTAATCAATGTTTGTGCAAGTGTAATTTTGTTTTGTATCGTAATTTTTTCATGCGTTCTTCAATTTGTAAATTAGAGTTGATGATGTCGTCTATTTGGTGGAATTATCGTTATTGGATATAGTTTTTCCGCGAAATTTGTGAGGATTTTTTTTGTTCAATGTTTTCAATTCATTCATTTTCTCCCGCACTTCCATTGCCTCCTCCTCCAATCACGAAGGTCTGTCTGTATGTCGCTTTCTCTCTCCTCTTCACCTATTTCATCTATGGCTTCGAATGCCGGCTCTGATCAATCGTTTGTGCAACTGCAATTTCGTTTTGTTTTCGTAATTTCATTCATTCTTCAATTTGAAAAATGAAGTTGATGATGCCGTCTATTTGTTGGAATTATCGTTATTAGATATAGTTTTTTCGCGAAATTTGCTAGGATTTTTTTGGCAATGTTTTCAATTCATTCATTTTCTCCCGCACTTCCATTGCCTCCTCCTCCAATCACTAAGGTCTGTCTGTCGTTTTCTCTCTGCTCTTTACCTATTTCATCTATGGTTTCAAATGCCGGCTTTGATCAATCGTTTGTGCAATCTTAATTTCATTTTGTATTCGTAATTTCAGTTGTTCTCCAGTTTGTAAATTCAATTTGATGATGTCATCTTTTGGTTGGAATTAGAGTCATTGGGTATAGTTTTTTCGCGAAATTTGTGAGGATTTTTTGTCAATGTTTTCAATTCATTCATTTGAGCTTTTATTCAGAAAAAGAATCCAATAATTTGAGCTCTACATTTCCATTGCGGCTTTAAAGTTCGACTGGAAATTGAAGTATAAGTTTAGACAATGGAAACAGGTTGAACTGTGATCAATTTTCGCCGATCAGGGTGAAAATTCTTCAGTCAAGAACACCTTGTTGTTAGGGTCAAATCAGCTGAGCTGTAGAGGACTAGAGGTATACTAGTAGTGTGGAATCATAGATATAATAAATTGTCCCGTTTTGTAAAGCTGAATACTAGGTTTGTCATGTAGTTTTCCAATCACATCATAAATGATTTGGGCATCTATTGCTGCCGCATTTAAGCTCCATTTGGCTTTGACGTTAAACAGTTTTACAAGAGGAAACATAACCACAAACAAGTTCTCTACTGTTTGGTTCCTTAAAAGGAATGCTATAGAGGAAAAGAAAATGGAAGTGAGAGGGTGGATGAGAGGAGAGAAAAAGGGAGGTAACTAGTAGGGAAATAAATGTGGCTTCCATTCCTTTCAAAAGAAAAATGTTTTTCATCCTGGTGAAAACTGTATACCGCTCCTTTTAAAAACCAAACAAAGGAAAATAGAAAATTATTTCCTGGAAAATCTTTAACACCTGCCAGGCTGCCATCTAAGTTGAAAACAAGATTGGGAATGATGAAACCAGATAATCTTGGGCAACTAGGGTTTACTTGATTAGACTCGAGCTTCTTTGCTTGCGTTGAAGTTAAACACGAAGTTTGAGTAATCAGCTTATCATGATTTTGTTAGTGTCCCATTCTGGAAGGTGATAAGAATAGGGAAGAAAACATAGAACTAATGAGTTTGCAAGATAGAAAAATGGCCAACTTTTCTGGTAATGCTCATAATCATAGGAATTTCCCTTTTACTGTCTAGAAGGGCGTGTAAGATCTTTGTTTCCATAGTAGCCATCCACCATCAAGTCACCCAGGTTGTGTTTCTAGAGAACTCTTTTGAGGGAAAGCTGGTTAAGAAGCCAAATCTTACTAGCCTCAACATGTAGTAGATGTGATGCTTTCCTAGTTGTCTAGGTGGACAATGTGTATACTGTGGAGTATACTACTTTTCTTTAATTGATGCATTCAAAGAAAAAGTATGCTGCTTACTTTTGTTCTGTATTGGTTCTATACTGGCCATGCAATTGAATATCAACTTGTAAGAGATTTTGTCCCTTAGTAATTGCATGTGTACAATCATTTAAGCAAAAACGACGGTATTTTTAACTTGTAGTAAAAAAAAGTGCTAGTAATCGTTAAAATGATGAAAAAGTATGTGAAGAACAAAATTGGTCTTATCTAGCAGTGTCTCAGCTAATCTCTACTTTCATGCATTTTTTCTATCCATGTCAACAATACCCCAATTCCTCTAAAGAGGCTCCAACTATGGTATGCAGGGGGTTTCAGATTTGCTGGACACATCTTTATTGCTTCTTGATTCAAATTTCAAAACACATGAACTGTTTGCTATGAGTACTTCACATTAAGAAAGAAGCTCTTTTCCTCTTAATCAATCAAGTTAAAATTCCGATCCCTATCCCCTCGTGCTCTATGTTACTCAGACTCGGGTCCGAGTGTCGGATACAGGTACGTGTCCAAGTGTCCGACACGGCTAAATTGTGGAAAATTTCCTTGATTTTAGACCAAAATGAAGTGTCGGAGTGTCCATACCCATGTCCAAGTGTCGAGGATCCGACACGGGTACTTGAGGTAAAACGAAGAGTCTAAGTAACATAGCTCGTGCTAATAGCCTGATATTAGGGCATTTTGTATCAGGATTTTTCCCTCAAATTTTTTTTTGGGTACTATTTGGTGTGCTCTAACTCTCTATTTCCACATTTGCAGTTCAAGATTGCTCTTTGCCAGCTGTCAGTTACAACAGACAAATTGAGAAATATTTCACATGCTCGTGAAGCAATTGAAGAGGCTGCAAACAAGGGTGCTCAGCTTATTCTTCTTCCTGTAAGTAAACTTGACACAGTTGGGTACTTAGGTTGATGGTGCGATGAGATATGTTTACTTAACACATCAAGTGGGCGCTTTTTTGGATTATTTGAGTTGGCCTTTTCCTATCTATGGATTGTTGCAATATGTGATTATTTTTCTTAAAAAACGTGTCTTAATTTCCTTTTCTACCGGTTCTACGTGGATAATTTAGTAAAGCACGTTTTGCTTTCTCCATTCTTTCGTCTCAGGAAATATGGAACAGTCCCTATTCGAATGACAGCTTTCCAGTTTATGCCGAAGATATCGAATTAGGTGGTGATGCATCTCCCTCCACAGCAATGCTTTCTGAAGTTTCCCGGAACCTTAAAGTTACAGTAGTTGGTGGTTCCATACCAGAGAGATGTGGTGATCGGCTTTATAATACTTGCTGTGTCTTTGGGACTGATGGAAAGCTAAAAGCTAAACACAGGAAGGCATGTGTCTAATCTACTGTAGGTGTGGAGGAGACACTCTTTGCGTTCCATGATACTAAAGTTTATATCATTATGTCCCCTAACATTACTGCGTTCACTTTTTACAGATACATCTTTTTGACATTGACATTCCAGGGAAAATGACGTTTAAGGAATCAAAGACACTTACCGCTGGAGAGAGTCCTACCATTGTTGACACAGGTTTTCTACCTTTTCTGCTCTATGATGACTAGGCATTCTAGATATTATTGTGCTATAGTTGCTTATTAAATGTCGGGGTTAATTAAAGTTCGATACAGTAATATGATCATGAAGGATTCTTCTAGATATAGTAATGTGATTATGAAATTTCCTTTTAGTTAGGAGGGTAAGGGCTTTTGCTAATTTGCAACCTGTAAAGGGCTAATCAGATTTTTGGACATTCTTTTTCTACTTATTGTTCATTTACTTGCTGAAATAGGTATTCACTAATCTCGATTCGAGTCTAGTTTCGTTTGTTCAATGGATCCGTTTTAGTAGTCTATTCTTTTTTAATTTCTTTTCTGGAACTACACAAATCAATAAGTAGTGGTTCTCCTGTGAGTATATCTCTGGGGAGATAAGATTTTCTACTAGATCACAGTGATATGCAGATAATCCTTGGGTTCTCTCACCATGTTTCCTTATCACAGTGTCACACTGTGCTTGTAAAATGCTGAAATCACATATGTGATATGGCTTCTGAATAAACTTATCTTTGTTGTGTTAAAGGGCATTCAATCTGTCTAAGACTTCATTGAAATACTCTGAATTCATGAATCTATTTTGTTTACAGAGACCTGTGAATCTTTTCCTTTCAAGCAACGTCTTCCAGAATCTTAAGACAGGAAGTTAGAGTTACTTAATACCTCTTACTTACAACTAAACTGATTTTGACCTCAATTGGAGCTTTTTATGGAACATTTGCGCGCGTAACACACACACACACACACAAATACACTAGTTCACTAGTTCTTGTCATACTATTAAAATCCTCCTTATATATACTTTGTGCTTATTTATCAATGATTTATAAGCATATACTATACAGTCCCAACTTTGCCAGAATCTTATATTTGAAGAAGGTCCTTTTCTCCTTTTTTTTGGCTCTATGTTGTTGTATTGTTATAGGCTGCTCCGGCACTTCGAGGGCTGATAAGTATTATTTTTGTACAGATGTTGGGCGCATTGGTATTGGTATATGTTATGATATTCGCTTTCAAGAATTAGCAGCAATTTATGCAGCAAGAGGTATGTTTTCACTTTTGGTTTATAGTGTGTTAGTTCTTAATATTGTTGATCTCTCCTTTCTCTTAACTTGCCTCTCTCTTTGTCTTCCCATCAATCCTTACCCATCTACTCATATTTTCCTTTCTTCTATGGATTTTTTTGTAAGGAACGAAACAAAGGATAACTATTTTGGGTTTTTTTTTTCATTTGGAAGTGTTTCCCATGGAAAATCATTTTCCCTGTAAATGTTTTCCGCCAAACCAAACGGAGCCTAGGCCAATTTAATACTGTAAACACAAATTTACGAGTCTGCAAAAATAACGAACTCACAAAATAAGTGAACAAAATATTTTATATCGATTTTTGTATTTGGGTACAATCTCTAATGTTTATCCACTGATTCGATCTCTACAACAGCAAGAACTTGTTTTGTCGGACGTCTTGAGCGAGGGCCACCGGACTTGATCTCTTTCAATGACGTAGTATGGAGGTAGAACGGCGTTTGTGCTTGATGTTCTTCTTCCACCGAGATTTGATGTCGACTTGTATGCTTGGAAGCAGTCTTAGGGTTTTATGGAGAGTAGTGTGTTTTCTGAATATTACGTTCAGTGTCTGCCAAATGACTTGGAGAGAACTTTTTTATATCCTCTTATTAGGTTTAGGGTTTTTCTTTTGATAAATGGGCCTCTTTTCTTTAACCCAATTAACTAACTCATTTCTATTAAATGAACGTCATTAGACTCAAAATGGGCTTATTTGATTTATGGCCCAAATTACACAATTTATCCGACATTTAAATAATAGAAGTCAACATTTGACTTTTCCGGGACCAAAATTGATGACATGGCATTTATCAAATTTTTAATGCCTACGAATACACCATTACCAAGTGAGATCACATATTCTGATGTGCTCGTCATGTCATCTATAAATTTATAGTTCCAGTCCAACCAAGAAATCAATGAGTGGTCGACGCCAATCTTCTATGTCTATCTCATAGACAGTAACTGCACCTGAGAGTTCTTCAGCTTCAATTTCAGCTTCATCTTGAGGGTTTATCACCCATCGATTACAGACCGGGACCGACATGTTTTCTTCTGCCCCCAGTGCCAATGTGGCTGCGAGATTTGCAAGTGCATCCGCTGTCTTGTTGGCACTCCTTGGAACATGTTGCAACCGGACGCTCTCAAATTCTGCTAGCAATTGTGAAGCATGCTTGTGATAAGGAATCAAATCATCTTTCTTGACATCATAATCGTCGAGGAGTTGATGGATCACCAGCTTGGAATCACCATATACATCAAGGTCCTGGATTTTCATTTCTTTTGCCATTTGAAGTCCTAGAACGAGAGCTTGATATTCAGCAACATTGTTCGAGCATAAGTTGGTGAGCGTAAATGAATACGTGAAGACATGCTTCTCCGGTGATACAAGGATGACCCCTGCTCCAGCACCATCTTGACGAGCCGCTCCATCGAAGTACATTTCCCACGGAGGCAGGACATCAATGTAGAATACTTCTTCTCCGGGTAGATCATCAGGAAGCTCCCAATGTGATGGAATCGGATGTGCAGCTAGGAAATCAGCCAAATCAGCCAGAGCTTGTCCCTTAATCGCCTTTTGAGGTACATACACAACGTCATGTTGACTTATGAGCATAGCCCATTTAGCAAGTCGCCCGGATAACACTGGCCTTGATAAGATGTACTTTATGGGGTCAGCTTTGGAGATTACCTTCACCGTATGTGCTTGCATGTAATGCTTCAACTTCTGGATTGCAAAGATCAAAGCTAGGCACACTTTCTCGATAGGCGAGTAATTCAATTCCGCGCCAACCAACGTACGACTGAGGTAGTAAAGAGCAGTTTCTTTTCCTTCATCATTTTCTTGAGCACACAATGCCCCCAATGACCTTTCATGAGCAGAAATGTATAGGATAAGTGGCTTCCCTTGAACCGGTGCCCCCAAGACAGGCGGAGTTGAGAGGTATTGTTTGATGCTTTCGAAAGCTTTGCGACATGCATCGTCCCATTCAAATGGAGCACCCTTTTTCATAAGATGGCTGAAAGGATGACATCGTCCTGCAAGATTCGAAATGAAGCGTCGAATATATGCAAGTCTCCCTTGTAATCCGCGCAATTCTTTGAGGTTCTTCGGCTCGGGCATATTCTGGATTGCTGTAATTTTTGATTGGTCAACTTCAATTCCTCTATGTCTCACAACAAATCCTAGGAACTTTCCAGAGGTTACGTCGAATGCACACTTCAAGGGATTCATCTTCAATTGATACCTACTTCTCGAATACCTCACGGAGATGAAGCAAATGATCGTTCCTCTTCTATGATTTGACGACAAGATCATCGACATAACATTCAACGGTCTTATGCATCATATCCTCGAAAATTTTCTGCATTGCTCTTTGATAAGTTACGCCTGCATTTTTCAAACCAAACGGCATCACCTTGTAGCAAAAGATACCATTTGGTGTACGGAACGCCGTTGCTTCTTGATCTTCGGGCGCCATGAGGATCTGATTATATCCCGCCGTACAATCCATGAAGGATAATGCTTCATGTCCGGTCGTTGCATCAATCATTATCTCGGTGACAGGCAAAGGAAAATCATCCTTAGGACACGCTTCATTCAGATTTCTGAAATCTACACAAACACGCAACTGGCCATTTTTCTTCCTCACAGGGACAATGTTTGCAATCCAAGTAGGATACTTTACTTCACGAGTGAAGCCAGCGTCGATGAATTTGTTGACTTCCGCTTCAATTTCCGGAATTAGTTCAGGTCGAAAGCGTCTTTGAGGTTGCTTCTTTGCTGAGACTCCTTTCCTTACTGCTAGATGATGGACCGCTACTTTGGGATCAAGACCCGACATTTCCCTGTAACTCCAGGCGAACACATCTCTATACTCGGTGAGTAGTTCAAAGTATCCCTTCTCTTCTTCCTGACTCAGCAGAGCACTAACATAGATCGGACGCGGATCATCTGGGGTGCCGAGATTCAACTCCTTTAGGTCATCAATTGTGGTCTCAACTCCATCATCAAGAGTTTTTGGTGCTTCTGTTGTTTCCATCTCCTCCTCCAAATCTACATCCTCATAAGCGGTGATATGATAGGAGGTGGCCGCCATATCTTCTACGGCATCACATTTCTGGCTTGTGGGGCTCTTTTGATTGGTAAGAACCATGGTGTGGCCTTTTACACGGAGAGAATCCCCTGTCTTCACTTCCAGTACCGCCAAGCGTTTCATTCGAGAAGGTACGTTGCTTCTGACATCGTTTTCCAGCTCGAATTTATCTTTCGTTTTTTATGCAGTGTTTTTTAGCCTCCATTGTGGCTTGAGTGAAACGTCCCTCTTCCCGAGACGATTGAAGACTTCCTTCTTAGGCTTATGTGACTGACAGTTGGTGGGTTTGCCAGTGATTTTTCCCAATCTTGTGAAGACATCCTTTGTATTCGGGCTTTTATGTGCTTTCCCGGACTGATCTCCCAATCTATCAAAGACTCGACTTGGTGTTGGAGAGTTTACCGGACCATCGAGCCTATCAAAGACGGAGGACCGTGCTTTAGCTGAAGACAGATGTATCCTATCAAAAACTGATTCCTTCTGGACTGGCTCAAAACTCTCATCCACACTTTCTTCAATTTCCTGAACTCCAATGTATTGTGATGCTTGCATTGACCTCCGTCGTCCTGAGATGACGATTGGTTTAGACTCCTTGAATCCCAAACCAACCTTTTTCACCCAAACACGTTCACCTTGTTGCTGAAGTTTTTGCTGAGTTTTATTGAGGCCGTACGACTTATCTTCAACGAGCTTTCCCATGGGTTCTGGGTTAGAGAAATCATAACCAGAATTCTCCATTAACTTGTATGCAACTGGATCAAAGCCATCTTTCCTTGAGCTAGGTGTTTCTGGTGACTTCGTGGAGTTTGGAGTAGGAGAAGTCAATATCTTCGGGTCACTGATCTTATGGACCGGTAATGTCATGCTTTTATCAAATTCTTGCGGCATGTCCTTGACCTTCTTTGAAGTTGTTTCTGCTCCACAATTCACAAACGGAGACTGACCTTCTTTGCGACGCGACATGGGAATGTAACGTAGCACTGGAACTGCTGATCTAGGATTTTCTTCAACTCTTTGCGAAGGAATCTCCTTGGTTTTCTTCTCATCAATTGTTTCATGTGACGGCTCCAGAGCATTCGTTGAAGTCTTCTCTTCTACCGTTGATTGCAACTTCTTCGGCATGACTTTTCCAGTCGATGCGATTTCCGTGGGCATTACTTCAACAGGCTCTGCCTGGCTGGCATAGAATTTCGCATCGGCAAAATGAGACTCTGCTTCAGAGAATGGCTTCACGTCCGCATTCACCTTTCTTTCACCATTTCTGTAATATTTGAAGCATAAATGCAAGGTTGAGGCTACTACTCCATTTTCGTGCATCCAAGGTCGGCCTAGCAATAGACGATATGAAGTGTTCGAATCAATGACATGAAAAATTGTTGTTGCAGCCATCTCCCCGATAGTCAGATCAACTCTTATCATACCGATGGCTCGTTGTCCTCCTAGATTGAATCCTTGAATCATCAACCGACTTTCGGTCAACTCACTTTCGGTGATTCCCAACTTCGTCATGGCGGACTTCGGCATTATGTTCACTGCGGAACCTCCGTCTACTAAGATGCGATTGATCTTTTTCTTGCGAATGTATCCACACACAAAGAGAGGTCTGTTGTGAGGCTTTGAACCCAACATAAGGTCTTCGTCAGTGAATGCTAAGGCCGCGCTGCATGCAGCACAACGCTCATGGTGGTTTTCGTAGATTCCTTGCTCAATATTTTCCTTCGACGTATATTGCGTAGGATTTTCTATGGCACGTGCAAGGGCGAACCTCATTTCTTCGGGAAGGGATAAAGCTTGGCGCCAACCCATGCGAGAAGGGACTGTACCTAACAACGCCAGGATCTTTGCATCTCTTTCAGGGTCTCTCTCATGGTTTAGCCCTTCAGAGACTTGGTTCTCTTCCTTTTTTTCTTCTTCCGCCACGCTACAGGTTACTGTGTTGACAGAAACTTCGCCAAAGTATTCCTGCGGAAAGAAGTCATTTAATGTGATATACTTTCGTACCTCCTTTGTGGGCGAACTGTTGCGAGGGGAGCTCCTCTTCTTCACGAGACCTTTATTTCTTGCTACACTCGAACTTTTGGATTTGATACTTCTCTGGTGTTGCTTTTGACGAAGAGGAGGTTGTGCTTCTAGTTTCGTTTGCTTTCTCTTCTTCCTCCTTGTCACTAATGTCCATCCCTCTTCATCTTCTTTGCGAGGTAGAGGGGTGGACGCAAAATTCTTGACGGGATCCATTATCAAAATGGGATCCAAACTTCCGAACTGGATGGCTGAAGCATGATGGGACTCAGCCGTCCCATCTAGATCTAGCACGATCCTTCCTTCAGTTGCAAGTTGCATGATTCTCTCCTTGAGAGTGATGCACTTTTCGATAGGATGGCTCACTATTCGATGATAAGGGCAAAAGTTTGGGTCGGATGTCCTTCCCATTTGATCAGGGCGCTTAGACTCAGGCAAGCTTATGACCTTCTTCTCCAACAAATCGCCCAACATGCCGGCCAAGTCTGAATCCGGAAATGGATACTTCTTTGCTTGGAGTTCTTTAAGAGTTGGCTTATCTCTCCGAGCATCCTTTGAAAATGACCCTTTCTTCTGTTCAGTTTTAGGCTTCGCCGAGATTTTTACAGGGCAGAGCTAGTGGCAACGGCAAATGACTCCTTGGTTGAATCTTTCACGTATTTCTCGGGCTTCTTGAAGTCCTTCTTTTCCTTCTTGAAACTTGAACTTCCTCCACGATTATTGGTCATACTGATCTCCATGTCATGAGCACGGGTAGCCAAATCTTGAAAGGATCTTGGTTTTATGCCCTGCAAAATGTACAACAAATCCCAGTTCATACCTTGAATACACATTGCAATAGCCGAAGTTTCCGTCAGACGATCTTTGCATTCCAGGCTTAACGTGCGCCATCGATTGATGTAATCTACGACTGGCTCGTTTTCCCATTGTGTCGTCTTTATCGATTCAATCATGCTGACGACACGTCTTGTACTGTAGAACCTGGCCTTGAATTCTTCCTCCATTTCTGCCCAACTGTCGATGGCTTTTGCAGGAAGGTCCGTGTACCAGTCGAATGCTGTGTCCTTTAGAGAATGGACGAACTGTTTGACGAGTAGATCTCCCGACGTGCCAGCATTGTTGCACGTTTCGATAAAGTGGGCTACATGCTGCTTGGGGTTCCCTTTTCCATCAAACTGCTGGAACTTCGGAGGTTGATAACCGACGGGCATCCTCAAGTAATCAATCCTCTTCGTGTACGGCTTTTCATATAGATGACTGGGGCCGGAATCTCCAGTGATGTGGTTCTTCACCGCATTCGTTACCATATCCTGGATCTTTTTCATGGTGAATGCGTCGTCAGGTTTTTCACCCTTTTTCGTAGATTTTGAAGCCGTACTCCCTTCAGAGTCGCCGTTTTCTTCGTCCGTGCTATCTTCCTGTTTATTGAGCTTTTTGGTCAAGGAGGAAATCTTCTCATTCTGCTTCTCGATTTTCTCGTCCTTCTCTTTGCCATCCTTCATGAGCTTCATGATCATGCTCTTCATTTCGGCGAGCTGCTCTTCAACGGTCTTTGATTCAATCGTCATCACCTCCGCAATGTCGGTTTGTAGGGACTGGGCCGATTCTTCACAATCTGACACTTTGAGGGGTGACGTTGGAGAAGACCCGGTACTACTCTTTGCATCACTTTCTACATAGAAAGGCTCGAACAAAGGACTGGTGTGGATGACCAACCTGTCAGGCTTTTGGTGAGGACGCATATCTTCTTCACCGGGCATAAGGAATCGTGCACCTTCAGCAGGAAAGGACCCGAAAACAGGGATGTTGCGGGACTTGCGGTAATACGGATTCGGGGACACGTCGGAGGAGGCACGGACATGGCTCTTTCTCCTTGTTTTGGTGGTGATAGGCGCCGAACGCAGACTTGAAGCAGCCTCATTCAGATCAGCGTTCTTCATTCCTCTGGTGCGTATACCCGCGTCACGCCCTTTCATCATCTCAGCCAATTCCGCCGCCCTTTGTTTCACAATAGCGGTCTTTTCGGCATCGGACATGGAAACGGTTTGTTGAGCAGCCATCTTTGTTGGTAGATTGATTTTTTTTGTAGAGAAGGAGATGAGTGGTAGAGTTGTCCCACCGAGCGTGACAAATTTGTAAACACAAATTTACGAGTCTGCAAAAATAACGAACTCACAAAATAAGTGAACAAAATATTTTATATTGATTTTTGTATTTGGGTACAATCTCTAATGTTTATCCACTGATTCGATCTCTACAACAGCAAGAACTTGTTTTGTCGGACGTCTTGAGCGAGGGCCACCGGGCTTAATCTCTTTCAATGACGTAGTATGGAGGTAGAACGGCGTTTGTGCTTGATGTTCTTCTTCCACCGAGATTTGATGTCGACTTGTATGCTTGGAAACAGTCTTAGGGTTTTATGGAGAGTAGTGTGTTTTCTGAATATTACGTTGACTTGAAGAGAACTTTTTTATATCCTCTTCTTAGGTTTAGGGTTTTTCTTTTGATAAATGGGCCTCTTTTCTTTAACCCAATTAACTAACTCATTTCTATTAAATGAACGTCATTTGACTCAAAATGGGCTTATTTGATTTATGACCCAAATTACACAATTTATCCGACATTTAAATAATAGAAGTCAACATTTGACTTTTCCGGGACCAAAATTGATGACATGACATTTATCAAATTTTTAATGCCTACAAATACACCATTACCAAGTGAGATCACATATTCTGATGTGCTCGTCATGTCATCTATAAATTTATAGTTCCAGTCCAACCACTTTCTGAGTGTCTGGATATCCACTTAGCTCCGAGTTTTTAATGGGTTTATATCGTACATTTGTACACCCTTTGTTCCCTTAATCTTTCTCTTTTGCTTTCTGAAGATGAATTTTTATTGTTAACTTCTCTTAGAATCTATAAATTGAAATTTAATAAAAAGTGTATTGGCTGTTTCGAATTAAAAGGTAACTTTAATAATATGTGCAGCGGAGGGATTAGTTCATAGTTTGAGGTTTCTTTTTAGATAACCAATAATTCTTTTCACCGCTTGCAGGACCTAGATAACATGCTTACACCATACGTCTTATCTGGTCTTTTAGCCAAATTATGCTTTTTTATTGCTTTATATTATCCCCTTCTGAATCGTACTACTCTACCGTCTTGACTGTTATTCTGAAATTTCGAATAACATTGATTAGCGAAATGTCCAAACAGTAGTAGTCTAATGTCTAAGCTCTCTATATTTCATTTGTTATTCCTTACTGTTGTAGAGTCTTGCCAACTAAAGAAAACTTCCGCGAACTTCAGTCTAATCCCAAATTTTGAAATGTAGGGAGCTTTTGTAATTTCAGGTATTTGCACAATTGCACTATATGAAACTGTTCTTGTATCCCATATCGTGGTAGAACTCAATGCCACTTTTGAAATTCCACTAAGTAATAGTCAAACATCTACTTTACCTCCTAAAACCACCTACAAAAATGATTGAGAAGATAGATGAAGGCATGCGAATCATGCAATATTACTAGGAAGTTGGAACTAGACTCAAATGACTCATGCTTCAATTTCTAATTTCCATCATCCAAACATGGAGCATATCAAGAGGAAGAAGATAAAGTCGTTAGTGACAGAAAGTCCTTGGCTTTTATTGACTACTTAATGCTTGGCCTTGGAAATGTGAGGCATTCCTTATCTACAATATCATATAGGCAGGGGCATCACTACTCACTAGAACAGTTTGGGATCAAAAATATGTTCAGTAGCTTCCCCCAGTTTGTAACAAACATGAAATGCACTGATCTGTTCCTTAAACCTTCCACAGGCTGTGATATTTGTCCAATTGTCAATGCAACCCCTGCCAGTGATTGTGGCTTTTGTCTACATCTGCTTCCTCTATAGTTGTCGAATTGCATGAGTACGCTGCAAAAGGCTCTGATTTTTCCTATTTAGAGTTTCTAGCGTAGGCATCCATATGTTAACCCAAGGATCTAGATTCTATAATACGCTATTGATTGATTCGGCGGATAATAAGCATGTTATCAACTTGCAAAAATCACCAGAAAAATGTCCTTGTCCAATTTCCAGTTTCATATGGAAATAGAAATTCCTGAACAAAGTTAAGGGCATCCCGCAAACTGTCCGCTTGGAGAGGCGCAGTAGCCAATACCTCTGGTAGATTTCAGATTGGCATTAGAAAAAAAGAGAGAAGCGTCCTGGATTTGTTTTGTGTTTAGAGAACTTCAAATCATATTTTCTGCATAGCTAGATATCGTTTTGGGAATTTGGGGTTCAGTGGGTGCATTCATTTTCTGCTTTATCGCATATGTGGATTAAGAATGTTCAGTTTGGAAGATTTCATAACTTATTTGTGGGGCTCCGTGTAATTGGGTATCCTTTAGTGCTTTAGACTGTATAATTGGAGAGAAATGCCAGAAATGCTGTAATATAAGCAATAGTTCTGAAGCTCATTAGGACAATTTGTATACCTTCCATCATTTTACTGTTGTTTATTATTCCTGGAATACTGCTTAATCTGTGAAGTGCCGTTGCACACTATTTGTGGGACTCTTTGGAGGCTTGTCCTCTCTGGATACATTCTGGTTCTCTACTAAAATAGAAAATTTTAAAGGTTTCGTCATATTTGAGGGCTTTCTGATTTTCTTTGATGTTGCCTCTTGTCACACTCATACATGCCACAATCGTTGAGCATGGGCCTTTTTTACTTCAGGTCTATGTTATTTACTTATTTGTCAATATTGATTCTTTTCAAGGTTCTGTAATTTTGAAATGATTTCTCAGTTCTGTTCTTGCATCTGAGTTTTCAATGTTATGTGGTTTATAATTGACGCTTATTGAAAAATCACCCTTTTGGTAGAGTTTAACTGGTACTAGGTTTTTGTTCTCTGTGCTTTTATTTTGACAGTGAAGCTGTATTGTGAATTTCAGGTGCTCATTTGCTCTGCTATCCTGGGGCCTTCAATATGACTACTGGTCCGTTACACTGGGAGCTACTGCAGAGAGCTAGGTAAGAGGTTCAATGCTTTCAGTAGAATCAATTTTCTCTGTTTTTTTTTTTATGCAAAGGATCGATTTTCTCAACCTTCCTAAACGACCAGAATCATCCAGTAGTGGGACTCACTTAGGGTACCATCTTGATTTTAATTGAAATAAGAATTGAGGTTTCAGCTTTGGTACTTCCTAGCTCGACTTATTTTCTGAGTGCTACCTCTTTTTGACATAATTTATGGCCTGTTTGGTATCACTGTCAGTTTTTGGTTTTTTAGTTTTGTAAGTCATATGATTTAGATTGAATCATGAACTGAAAATAGTCATACCTATAGTAATCATGTTGATTTTGAAAACGGACAACAAAAAACTGAAAACTACTTTTTGTGCTTTTCATATTTTGGTTTTTAGTTTTGTGAAAAACAGAAAACTAAAACAACAAACTATGCCGAATTTAGATTTGCAGCTGCTACAGCGTGTAAAGCTCAACTCTATCGCTTCTCGTTCCTAGGGCTTGTCCACTCAATAAAATGATGTTGTCCTGCTGAATAACAGGACTCGTTTTTACAAGGATATGTTAGCTATAGGCATGGTGCATTTTTCTGTTAATTCATGGCAGTACTTCTCGTTCCCTTGTAGGATGTAAAGAATTGGCAGATAAGGGGAGAATGACAAAACCAATATTTATACAACAATTTGTTTGATGTGTCTGGTCTAGAGATATTGTCCGTGCATTCATAATACTATTACACCTCAGAACTGGAAATAATGTTGCGGGGTAGGTGTTGAAGTTGACTGATTAGTTGGGGTCTGGCGGTGATTTGAATAGATATTCCTTCTACAATTTGCTTCTCTATGTCCACCCCTGCTCCCACCCCCAAAAAATAGGAAATTAAAATTGAGGGATTAGAATGGAGTGTCACGGTGCAAGTCGAATGAGTCATCTGACAGGAGAAGCTTCTGGAGGAGCACTCTTTGAATTTAGATCCTAGTGGTCCTACTCTTTAAAGTTGTCAAAGTATCCCCTAAAATGTTCCCCTATCAAGTTTTTGTATTATCACTTCCACCCTTAAATTGATAGGGTGAAAATTTCATGAGGACGAAAAAAATGATGGTTTTCCGTGAGTTTGACGAGCATCTTTTTTCATTTTAATGGAAAAACAATAACTAGTACTTAGTAGTTAGTAGTTAGTAGCAGACTAGCACTTTGTACCAGCACATAAATCAACAACAGTTGAGGGGACATGCACTAGAATGATTACTATGAAATGGATAAGTTCAATTTGTTAAAAAAGAAAATCTTGAGAAAAAAAGAGAAAGTCGTGTGAAGATATTTTGTTTCCCCATGTATGAGATTTTTTTTTGAACTTTTATTTTAGGAGGGAATGCCTATAATTCCTAGGATGGAATTTTGGAGGGTTGTGGTGAAAACTTGGAAAATTAACATTCTTATTTATACTGCGTATGTAGGATTGTTTTAAATGTAGTTTAACATATTTATTCACTCTTCTTCATAATATTAGGCACCCATGCTTTTGAAGTTGGACTTTACCTTTTTTCCCCCCCAAAATATAGTAATAGAAGCTACAAAAATTACACCATTAGATAACTTAGATCCATATATTATAGTTTCGTAATATAAGTTTTGATATTATCTCATATTTGGTGAAAAATTAACAGTCTAAGTTAAATCTTGAAAGCATGGTAGGTAATATAGCAATGTTGCCATCCCTGAACACTTTCTAATGAAAAAAGTTGAAATTGTATTGAAACTATACATGTCAAATTCTACTTAACCTTTTTTGTACGTGCTATACCCTCAAGTGTGGTTCCTTTTTCTAACAACGCCCGTTCTTTTTCTATGGAATATGCAGAGCAGCAGATAATCAGGTACTCCACTGACCTTGTTTAATGTTTATAAAATCACGAGAAACTTAATGTTTATACTAAGTTGTTGGTAAACTGCATAAATGATATGATACTACATTATTATTACGATGTCACAATCAAGGATTCAGGCTTCAGATAATTGTAGTAACTTCAAAAGTAGAATGATCTTTGCATTTAAAACTTGCATAAACTTGCACAAACATCTTCAGCCATCACAATTAAAGGGTCTGTACGTCCCATATGTTTCATGTCCTGGGAATGTGTCCATGTGTCTTCTTTAATTAGAAGGTGGATGATGTTCTTTACATTATTTTTCAACAGTTGTACGTAGCCACGTGTGCACCAGCTCGTGATACTGGATCAGGGTATGTGGCATGGGGACACTCTACCTTAGTTGGACCTGTAAGTTTGTCGAGTTTCCATGACTTAGTTGTACCTTCTTCAATTTCAGGCTATCACTTGATAAGGACATTATTTGTGAAATATAGGAAAATTAATATTTTGTGCATGACATTTGTTTATGGCAGTTTGGGGAAGTGCTTGCAACAACTGATCATGACGAGACAATTGTTATAGCAGAAATCGACTACTCAGTCATTGAGCAAAGAAGGTGACATCTTCTGTACTTTGTGTTAGACAGTTTGTTTGTACTGTCAATTTTTTTTAATGCTGTTACACGTGAACATAATTCTATCTTTGATACTCCCTCCGTCCCTAAAAGATTGCCCCAATTTCTTTTTCGGCCCGTCCCTTTGAGTTTGCCCCATATCATTTATAGTATGTGGTCCTCACATGTTTTCTCTCACATTCTATTAAAATTAATTTATGCAAAAAATTAATTTATAAAAAATACATATATTAAAAAGTACTCGAGCTTGTTCACGAGCTTTCGAGCCGAGCCATTGATAGCTCAGGCTTGGCTCGTTAAGTTCTCGAGCCGAACCTGGAGTCGAGCCTGCTCGAGCCGAGCTTCGAGTAGTTTGCGAGCAGGCTCGGCTCATTAACACCCCTATATGGGACAATCTTTTAGGGACAGAGGGAGACAAGAATGAGAAAGTTCCAGAAGTCCAGTCTTTTTTGTCTAAATGTTCCAAGAAAATCTGGTGTTGTATTTCTCATTTTCTGGAGGTTTTATCCTCTTTACTTCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCCCCCCCCCCCCCATCCAAGGCATTATACGCTATTTACCGTATGCTTGTGTGAGCTGCAGAACATATCTACCCTTCATCAACCAAAGGAGAGGTGATTTATATCAGTTAGTTGACGTTCAGAGGCTGTATGCCGAGTCTACCAAGTAACAGCACAGAAACCAGCTAATCATCATTGGCCCTTGGAGCCTCAGAAAGTCAGAAGCACGCACTATAGTCCCGAGGCGCTCTTCATTTGTCTTCCAAATATGATGATGAACTAGATAAGATATCTTTTGGTGGGATGACCATCTCAGGTACTTCAAATTTATGGTTTTCTTCTCTCTTTTTCGATTGCGTTATAGATTGTGCATAATTGTGATTCGACAATTTTGGAATGTAGCACTATGTGTGTATGATACAACTCATTAATACAGGAAAACTGATAATCAGGGCCAGTATACTCCCAATAATAGTAGTGTCATTAGTGGAGAGTTAGATTGTGGGCTCTAAATGAGCTGTAGCCTATAAATAGATACTACTACTGTATTACATTAGTGATCTAGGCCCATGAGGCCATGAGTGCACCTGATTTCATTAAATAGATGTGTCCAAGTGTTAATTGCCAAAAACATTAATTTCAGCTTTTTGAGAATATTGCCTATTTCAGTGAGTTCTTTCGTTTTTTACTCATATTAAGAGCTGTAGCTTGTTGGTGGCAGATAATTTGTTGGGTCACGATTCATACACTCCGCACACTTCACTAGTAGACCAATATATGACCTGAATTGAACCAAAAGATTTATCTCCTGATAAGGATGTCAGTGGACCCGCCCCGCATCCAAGGAGAAGTTTGGTGGGTGATGGGTAGGGATAGGTTAAAAAATCATACCCGTCATTGGTGGTGGGGCGCAGGTGTGGATTTTGTCACCCGTCCCCATCCGCCACGCTTGATACTCGTCAACTTGGATAGGGTTTGGATTTAAAGAAGATAAAAAGTTGAACAATTTTTTTAATGGGTTTGGATTTGTTTGTATTATGTGATAATATGAATTAAGGAGAGACTTTTAAGTTTGTTTGTTTGATTTTTTTTTTAATGCAGAATATACTTTTTTCCATAATGTTGTCAGGTGTTTGCTAAATAAGAAAAACTAGGTAGGTATTGGCGCGGATATGGGGTGGGGATGGATGCCCAGACGGCTGATGGGCGGGGATGGATTTTAGTTTGTACCCTTGGGGCGGGGAGGGGAATGAATTTTGTACTCGCAATGAGTGATGGGGCTGGTATGGAGGGACGTACATCCGCCAGACCGCCACTCCCCATTTGCATCATTNCGCAATGAGTGATGGGGCTGGTATGGAGGGACGTACATCCGCCAGACCGCCACTCCCCATTTGCATCATTCTCCCCAAATTGGGTTGATAAAAACCAACCTAATGTCCTAGTAACCTGTTTGCCTCGTCTAGTGGTGAACTTGCGTTTCCATTAACAATCTTGTCCACATTTTATGACCCCAGCAGCTCACATTATAAAACATAGCTCTCCATTACCATTTTTCGTCAACTCTGGCACTTGAATTAGTCATGTTTAAGATGCATGACTGATTGACTGTAGACGACTCAAAAGTCAAAACCCATGGTAAAACCTTGGTATGTGTAAGATGCACTGCACAATTTGTCCCTAAAATCAGCAAACCCTTTAGACTTGTTAGTTCAAACCTAAATTTCAAATGGGAGAACTCTGAGGTGAGTCTGAGGTAAGTCTGAGGATAATGGGTAAAATAGGAATAATCTTGTTTAAGTCCGAATAATGAAAAAGTAAGTGAATAACGACGTAACATAGTATGGAGTTTGAAGATGAATTTGAGGGATAAACTCGCCCTAAAAACTTAATCTAACTACCAACATTTGACCACCAATAGGGCGGTAGGGCTATAGAGCCTAGGTCCGTAAAATCCCCCCCACTATACACCCAACCCACCAACTTCTTCTACTTCTTATGTGAGACTGTGAGTTAGTTGGTGCCAATATATAATAGCTAAGCTAGGTAATCACCGAACTAACTACATTCATTTTTGTTTTTAATTACTAGTATAGGAAACCCTAGTACTCCTAACTTCTACCACAACATACAACTATTTAAACATTGGACATCGGGCAAATTATAATATTCGATCTAGTCAGATTAAGTAGTGTTCAATTATCAAGACGTTTGTTACTTACAATATACGCAACAGCGTAATGTGTGTTCAATTGGAAAAACTTACTCTTTACGGCCGTAGTATTCGTTATTTATTACTCCCTCCGAATCTAAATGGTTTTCCCATTTAAAATCTTGACAATATTCATAATGAAAGAGAATCTTATGAATTACGTTCAATATATAAGAAAAAACATAGTTATGTGGGGTTTTGTTTGATTCGTCTCAATGAGTACTTTGACAATATGAAAATTTTATAATTTTTAATAACGTAACTAGAGATATTAACGATGTGAAATGTGCATGTGTGCCAAAGGGAAATGGGAAAAACATTTAGATTCTGAGGGAGTATATAAATATTTCTAAGTAAATCCTCTACTCAATCCATTTATCAATAGAATTGTTGAGTTTAATCACATTGCATCAATCATCTCATGTCTTCAATGCGTGGGACAAAAGATCTGGACTTAACTAGTTTTTGAACGAATTATAGCAAGGTTCTATCTCTTTGAACTAATTTTTTTTTTTGGTTAAAAGACTAAAATCAAATGTAGTCGATTATTATTAAAAGGATGAAATATCCATCATAAATTCAGAGTCCTTTATGCCAATACAATAATCAGTGAAAATCAATGAGGAAAACATGCACCAGCCATATCAGCAATATCATCAGTTTTTCCTTGAAGGTTTTGCAACTTTGAAGACAATGCATCTCCAATTTCAACAACCTAGCTTCTTCCCAACTTCAGTTGTCTTCTTCTGCAAAACCTCGATTTCTTGTGTCAGATAACTGTAAAATTCTTCTACTATTGCAATACTTTCCTCCATCTTCTCCTTCATCTTCTCCTTCGTCTTCTCCTTCATCAAACTTTGTCTTTTTAATAACTCTACGTAAGAAAAGGCAATGGCACTTGCTTGCTCGTTAAGCTTTTCCGTGTGCTTCATGATAATGCCTAACATGTTCATCAGAGTTGCTCCTATTTTTTGTGTTACTTCATCAATCGATGTTATTGATTCTTGTGTTTGATTCAAGATATGGACAAGAGTATCTGTTTTTTCTTTCATGGTTTCTAGTTTTTTTTCTGTTGATAATGCTGAATCTTTCAAGTCTTTCCCCAGCCTCTCAACTTGAAGGTTAAAACCCTTGGGCCTGGCCGAAAAAACAGGGGAAAAAACACCAATCTCCTCGTTAGATTTCACACTTACATAAAAGGTTTTTTTTTTTTTATAGGTTCCGATAAATAG

mRNA sequence

TTCACGAGCAGACTCGGTTCGTTAACGCACCTCTAAACTCCTCACGACTCCACCGCGCCACAGCTAAAAACAACAAACAGCGTCGTTAACTCGTTATCACTTCCACAGACTTCACTCACGCTGAGAAGCACAGCACACAAGATGATCGCCATTACCCAAATCCAACCATTAACAACACCACACTCACTCCAAAATGCATATCACCAAACCAAAAACCTCTCATTTTACTCTTCTCAAACTTCCGTCGTCAAATTGCGCATTCCTAACTTCTCAAACCCCAACAAACCACCTCTTTCCTCCAGAATCGCCGCCATGTCCGTCGCCGACCAAGCTAGGGTTCCTCTCGCACTTCCTTTGCCTCCTCCTCCGGTCACTAAGTTCAAGATTGCTCTTTGCCAGCTGTCAGTTACAACAGACAAATTGAGAAATATTTCACATGCTCGTGAAGCAATTGAAGAGGCTGCAAACAAGGGTGCTCAGCTTATTCTTCTTCCTGAAATATGGAACAGTCCCTATTCGAATGACAGCTTTCCAGTTTATGCCGAAGATATCGAATTAGGTGGTGATGCATCTCCCTCCACAGCAATGCTTTCTGAAGTTTCCCGGAACCTTAAAGTTACAGTAGTTGGTGGTTCCATACCAGAGAGATGTGGTGATCGGCTTTATAATACTTGCTGTGTCTTTGGGACTGATGGAAAGCTAAAAGCTAAACACAGGAAGATACATCTTTTTGACATTGACATTCCAGGGAAAATGACGTTTAAGGAATCAAAGACACTTACCGCTGGAGAGAGTCCTACCATTGTTGACACAGGCTGCTCCGGCACTTCGAGGGCTGATAAGTATTATTTTTGTACAGATGTTGGGCGCATTGGTATTGGTGCTCATTTGCTCTGCTATCCTGGGGCCTTCAATATGACTACTGGTCCGTTACACTGGGAGCTACTGCAGAGAGCTAGGATCGATTTTCTCAACCTTCCTAAACGACCAGAATCATCCAGTAGTGGGACTCACTTAGGTTTTGTGAAAAACAGAAAACTAAAACAACAAACTATGCCGAATTTAGATTTGCAGCTGCTACAGCGTTTGTACGTAGCCACGTGTGCACCAGCTCGTGATACTGGATCAGGGTATGTGGCATGGGGACACTCTACCTTAGTTGGACCTTTTGGGGAAGTGCTTGCAACAACTGATCATGACGAGACAATTGTTATAGCAGAAATCGACTACTCAGTCATTGAGCAAAGAAGACAATGCATCTCCAATTTCAACAACCTAGCTTCTTCCCAACTTCAGTTGTCTTCTTCTGCAAAACCTCGATTTCTTGTGTCAGATAACTGTAAAATTCTTCTACTATTGCAATACTTTCCTCCATCTTCTCCTTCATCTTCTCCTTCGTCTTCTCCTTCATCAAACTTTGTCTTTTTAATAACTCTACGTTCCGATAAATAG

Coding sequence (CDS)

ATGATCGCCATTACCCAAATCCAACCATTAACAACACCACACTCACTCCAAAATGCATATCACCAAACCAAAAACCTCTCATTTTACTCTTCTCAAACTTCCGTCGTCAAATTGCGCATTCCTAACTTCTCAAACCCCAACAAACCACCTCTTTCCTCCAGAATCGCCGCCATGTCCGTCGCCGACCAAGCTAGGGTTCCTCTCGCACTTCCTTTGCCTCCTCCTCCGGTCACTAAGTTCAAGATTGCTCTTTGCCAGCTGTCAGTTACAACAGACAAATTGAGAAATATTTCACATGCTCGTGAAGCAATTGAAGAGGCTGCAAACAAGGGTGCTCAGCTTATTCTTCTTCCTGAAATATGGAACAGTCCCTATTCGAATGACAGCTTTCCAGTTTATGCCGAAGATATCGAATTAGGTGGTGATGCATCTCCCTCCACAGCAATGCTTTCTGAAGTTTCCCGGAACCTTAAAGTTACAGTAGTTGGTGGTTCCATACCAGAGAGATGTGGTGATCGGCTTTATAATACTTGCTGTGTCTTTGGGACTGATGGAAAGCTAAAAGCTAAACACAGGAAGATACATCTTTTTGACATTGACATTCCAGGGAAAATGACGTTTAAGGAATCAAAGACACTTACCGCTGGAGAGAGTCCTACCATTGTTGACACAGGCTGCTCCGGCACTTCGAGGGCTGATAAGTATTATTTTTGTACAGATGTTGGGCGCATTGGTATTGGTGCTCATTTGCTCTGCTATCCTGGGGCCTTCAATATGACTACTGGTCCGTTACACTGGGAGCTACTGCAGAGAGCTAGGATCGATTTTCTCAACCTTCCTAAACGACCAGAATCATCCAGTAGTGGGACTCACTTAGGTTTTGTGAAAAACAGAAAACTAAAACAACAAACTATGCCGAATTTAGATTTGCAGCTGCTACAGCGTTTGTACGTAGCCACGTGTGCACCAGCTCGTGATACTGGATCAGGGTATGTGGCATGGGGACACTCTACCTTAGTTGGACCTTTTGGGGAAGTGCTTGCAACAACTGATCATGACGAGACAATTGTTATAGCAGAAATCGACTACTCAGTCATTGAGCAAAGAAGACAATGCATCTCCAATTTCAACAACCTAGCTTCTTCCCAACTTCAGTTGTCTTCTTCTGCAAAACCTCGATTTCTTGTGTCAGATAACTGTAAAATTCTTCTACTATTGCAATACTTTCCTCCATCTTCTCCTTCATCTTCTCCTTCGTCTTCTCCTTCATCAAACTTTGTCTTTTTAATAACTCTACGTTCCGATAAATAG

Protein sequence

MIAITQIQPLTTPHSLQNAYHQTKNLSFYSSQTSVVKLRIPNFSNPNKPPLSSRIAAMSVADQARVPLALPLPPPPVTKFKIALCQLSVTTDKLRNISHAREAIEEAANKGAQLILLPEIWNSPYSNDSFPVYAEDIELGGDASPSTAMLSEVSRNLKVTVVGGSIPERCGDRLYNTCCVFGTDGKLKAKHRKIHLFDIDIPGKMTFKESKTLTAGESPTIVDTGCSGTSRADKYYFCTDVGRIGIGAHLLCYPGAFNMTTGPLHWELLQRARIDFLNLPKRPESSSSGTHLGFVKNRKLKQQTMPNLDLQLLQRLYVATCAPARDTGSGYVAWGHSTLVGPFGEVLATTDHDETIVIAEIDYSVIEQRRQCISNFNNLASSQLQLSSSAKPRFLVSDNCKILLLLQYFPPSSPSSSPSSSPSSNFVFLITLRSDK

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo03181.1	Spo03181.1	mRNA

Homology

[Close]

BLAST of Spo03181.1 vs. NCBI nr
Match: gi|902227977|gb|KNA20904.1| (hypothetical protein SOVF_048130 [Spinacia oleracea])

HSP 1 Score: 454.5 bits (1168), Expect = 2.100e-124
Identity = 234/253 (92.49%), Postives = 235/253 (92.89%), Query Frame = 1


		  
Query: 1   MIAITQIQPLTTPHSLQNAYHQTKNLSFYSSQTSVVKLRIPNFSNPNKPPLSSRIAAMSV 60
           MIAITQIQPLTTPHSLQNAYHQTKNLSFYSSQTSVVKLRIPNFSNPNKPPLSSRIAAMSV
Sbjct: 1   MIAITQIQPLTTPHSLQNAYHQTKNLSFYSSQTSVVKLRIPNFSNPNKPPLSSRIAAMSV 60

Query: 61  ADQARVPLALPLPPPPVTKFKIALCQLSVTTDKLRNISHAREAIEEAANKGAQLILLPEI 120
           ADQARVPLALPLPPPPVTKFKIALCQLSVTTDKLRNISHAREAIEEAANKGAQLILLPEI
Sbjct: 61  ADQARVPLALPLPPPPVTKFKIALCQLSVTTDKLRNISHAREAIEEAANKGAQLILLPEI 120

Query: 121 WNSPYSNDSFPVYAEDIELGGDASPSTAMLSEVSRNLKVTVVGGSIPERCGDRLYNTCCV 180
           WNSPYSNDSFPVYAEDIELGGDASPSTAMLSEVSRNLKVTVVGGSIPERCGDRLYNTCCV
Sbjct: 121 WNSPYSNDSFPVYAEDIELGGDASPSTAMLSEVSRNLKVTVVGGSIPERCGDRLYNTCCV 180

Query: 181 FGTDGKLKAKHRKIHLFDIDIPGKMTFKESKTLTAGESPTIVDTGCSGTSRADKYYFCTD 240
           FGTDGKLKAKHRKIHLFDIDIPGKMTFKESKTLTAGESPTIVD               TD
Sbjct: 181 FGTDGKLKAKHRKIHLFDIDIPGKMTFKESKTLTAGESPTIVD---------------TD 235

Query: 241 VGRIGIGAHLLCY 254
           VGRIGIG   +CY
Sbjct: 241 VGRIGIG---ICY 235

[Close]

BLAST of Spo03181.1 vs. NCBI nr
Match: gi|604315785|gb|EYU28350.1| (hypothetical protein MIMGU_mgv1a022881mg, partial [Erythranthe guttata])

HSP 1 Score: 438.0 bits (1125), Expect = 2.000e-119
Identity = 230/343 (67.06%), Postives = 261/343 (76.09%), Query Frame = 1


		  
Query: 51  LSSRIAAMSVADQARVPLALPLPPPPVTKFKIALCQLSVTTDKLRNISHAREAIEEAANK 110
           +SS   A+  +D+ARVP A+P P PPVTKFKIALCQLSVT DK RNI+HAR+ IEEAA K
Sbjct: 84  ISSATTAIMGSDEARVPPAIPPPTPPVTKFKIALCQLSVTADKERNIAHARQVIEEAAGK 143

Query: 111 GAQLILLPEIWNSPYSNDSFPVYAEDIELGGDASPSTAMLSEVSRNLKVTVVGGSIPERC 170
           GAQLILLPEIWNSPYSNDSFPVYAEDI+ GGDASPSTAMLSE+SR+L++T++GGSIPER 
Sbjct: 144 GAQLILLPEIWNSPYSNDSFPVYAEDIDAGGDASPSTAMLSELSRSLQITIIGGSIPERS 203

Query: 171 GDRLYNTCCVFGTDGKLKAKHRKIHLFDIDIPGKMTFKESKTLTAGESPTIVDTGCSGTS 230
           G++LYNTCCVFGTDGKL AKHRKIHLFDIDIPG+MTFKES TLTAGE+PTIVD       
Sbjct: 204 GEKLYNTCCVFGTDGKLMAKHRKIHLFDIDIPGQMTFKESNTLTAGETPTIVD------- 263

Query: 231 RADKYYFCTDVGRIGI------------------GAHLLCYPGAFNMTTGPLHWELLQRA 290
                   TDVGRIGI                  GAHLLCYPGAFNMTTGPLHWELLQRA
Sbjct: 264 --------TDVGRIGIGICYDIRFQELAAIYAARGAHLLCYPGAFNMTTGPLHWELLQRA 323

Query: 291 RIDFLNLPKRPESSS-SGTHLGFV----KNRKLKQQTMPNLDLQLLQRLYVATCAPARDT 350
            +    L    +SS  SGT    +       KL+   + + +L  ++ LYVATC+PARD 
Sbjct: 324 SLVLTCLLLDVQSSRLSGTLASLLILSKMTFKLQSGDITSQNLICVEALYVATCSPARDC 383

Query: 351 GSGYVAWGHSTLVGPFGEVLATTDHDETIVIAEIDYSVIEQRR 371
           GS YVAWGHSTLVGPFGEVLATT+HDE IVI+EIDYS+IEQRR
Sbjct: 384 GSSYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSLIEQRR 411

[Close]

BLAST of Spo03181.1 vs. NCBI nr
Match: gi|698501289|ref|XP_009796352.1| (PREDICTED: omega-amidase,chloroplastic-like isoform X1 [Nicotiana sylvestris])

HSP 1 Score: 431.4 bits (1108), Expect = 1.900e-117
Identity = 228/347 (65.71%), Postives = 252/347 (72.62%), Query Frame = 1


		  
Query: 42  NFSNPNKPPLSSRIAAMSVADQARVPLALPLPPPPVTKFKIALCQLSVTTDKLRNISHAR 101
           NFS  ++  +S+ + A   A++ARVP ALPLP PPVTKFKIALCQLSVTTDK RNI+HA+
Sbjct: 44  NFSKFSRSIVSATMEASFKAEEARVPAALPLPTPPVTKFKIALCQLSVTTDKERNIAHAK 103

Query: 102 EAIEEAANKGAQLILLPEIWNSPYSNDSFPVYAEDIELGGDASPSTAMLSEVSRNLKVTV 161
           +AIE+AA KGAQLILLPEIWNSPYSNDSFPVYAEDI+ GGDASPST MLSEVSR+LK+T+
Sbjct: 104 QAIEDAAGKGAQLILLPEIWNSPYSNDSFPVYAEDIDAGGDASPSTRMLSEVSRSLKITI 163

Query: 162 VGGSIPERCGDRLYNTCCVFGTDGKLKAKHRKIHLFDIDIPGKMTFKESKTLTAGESPTI 221
           +GGSIPER GD+LYNTCCVFGTDGKL AKHRKIHLFDIDIPGKMTFKESKTLTAG  PTI
Sbjct: 164 IGGSIPERTGDKLYNTCCVFGTDGKLMAKHRKIHLFDIDIPGKMTFKESKTLTAGVRPTI 223

Query: 222 VDTGCSGTSRADKYYFCTDVGRIGIG------------------AHLLCYPGAFNMTTGP 281
           VDT               +VGRIGIG                  AHLLCYPGAFN TTGP
Sbjct: 224 VDT---------------EVGRIGIGICYDIRFQELAAIYAARGAHLLCYPGAFNTTTGP 283

Query: 282 LHWELLQRARIDFLNLPKRPESSSSGTHLGFVKNRKLKQQTMPNLDLQLLQRLYVATCAP 341
           LHWELLQRAR                             Q         L +LYVATC+P
Sbjct: 284 LHWELLQRARA-------------------------ADCQIWSYFFCTYLNQLYVATCSP 343

Query: 342 ARDTGSGYVAWGHSTLVGPFGEVLATTDHDETIVIAEIDYSVIEQRR 371
           ARD+GS YVAWGHSTLVGPFGEVL TT+H+ET +IAEIDYSVIEQRR
Sbjct: 344 ARDSGSSYVAWGHSTLVGPFGEVLVTTEHEETTLIAEIDYSVIEQRR 350

[Close]

BLAST of Spo03181.1 vs. NCBI nr
Match: gi|731372813|ref|XP_010666455.1| (PREDICTED: omega-amidase,chloroplastic-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 429.9 bits (1104), Expect = 5.400e-117
Identity = 240/372 (64.52%), Postives = 268/372 (72.04%), Query Frame = 1


		  
Query: 1   MIAITQIQPLTTPHSLQNAYHQTK--NLSFYSSQTSVVKLRIPNFSNPNKPPLSSRIAAM 60
           M AI+QIQ L+T  S+QN   Q    NLS+ SS+T  V  R  N S   K  L ++I+AM
Sbjct: 1   MNAISQIQ-LSTSISMQNLKPQNTIINLSYSSSETFDVSFRKRNPSISRKSLLFTKISAM 60

Query: 61  SVADQARVPLALPLPPPPVTKFKIALCQLSVTTDKLRNISHAREAIEEAANKGAQLILLP 120
           S + QARVP  LPLPPPPVTKFKIALCQL VT DK RNI HAREAIEEAANKGAQL+LLP
Sbjct: 61  STSHQARVPPPLPLPPPPVTKFKIALCQLLVTADKSRNIFHAREAIEEAANKGAQLVLLP 120

Query: 121 EIWNSPYSNDSFPVYAEDIELGGDASPSTAMLSEVSRNLKVTVVGGSIPERCGDRLYNTC 180
           EIWNSPYSN SFP+YAEDIELGGDASPSTAMLSEVS+NLK+T+VGGSIPERCGDRLYNTC
Sbjct: 121 EIWNSPYSNVSFPIYAEDIELGGDASPSTAMLSEVSQNLKITIVGGSIPERCGDRLYNTC 180

Query: 181 CVFGTDGKLKAKHRKIHLFDIDIPGKMTFKESKTLTAGESPTIVDTGCSGTSRADKYYFC 240
           CVFGTDGKLKAKHRKIHLFDIDIPG+MTFKES TLTAGE+PTIVDT              
Sbjct: 181 CVFGTDGKLKAKHRKIHLFDIDIPGQMTFKESNTLTAGENPTIVDT-------------- 240

Query: 241 TDVGRIGIGAHLLCYPGAFNMTTGPLHWELLQRARIDFLNLPKRPESSSSGTHLGFVKNR 300
            DVGRIGIG   +CY   F          +        L  P     ++   H   ++  
Sbjct: 241 -DVGRIGIG---ICYDIRFQELAA-----IYAARGAHLLCYPGAFNMTTGPLHWELLQRA 300

Query: 301 KLKQQTMPNLDLQLLQRLYVATCAPARDTGSGYVAWGHSTLVGPFGEVLATTDHDETIVI 360
           +               +LYVATC+PARDTGSGYVAWGHSTLVGPFGEVLATTDH+ETIVI
Sbjct: 301 RAAD-----------NQLYVATCSPARDTGSGYVAWGHSTLVGPFGEVLATTDHEETIVI 337

Query: 361 AEIDYSVIEQRR 371
           +EIDYS+IEQRR
Sbjct: 361 SEIDYSLIEQRR 337

[Close]

BLAST of Spo03181.1 vs. NCBI nr
Match: gi|728815404|gb|KHG01832.1| (Omega-amidase NIT2 [Gossypium arboreum])

HSP 1 Score: 426.4 bits (1095), Expect = 6.000e-116
Identity = 234/385 (60.78%), Postives = 269/385 (69.87%), Query Frame = 1


		  
Query: 12  TPHSLQNAYHQTKNLS---FYSSQTSVVKLRIPNFSNPNKPPLSSRIAAMSVADQARVPL 71
           +P S    YH +K +S   F+ S T               P  +S +A++S ++QAR P 
Sbjct: 14  SPISRTLLYHSSKPISLSPFFLSNTPKNLFNQRYQKLQFSPNSTSIMASLSKSEQAREPP 73

Query: 72  ALPLPPPPVTKFKIALCQLSVTTDKLRNISHAREAIEEAANKGAQLILLPEIWNSPYSND 131
           ALPLP PP++KFKI LCQLSVT DK RNI HAR+AIEEAA KGAQL+LLPEIWNSPYSND
Sbjct: 74  ALPLPSPPLSKFKIGLCQLSVTPDKERNIKHARKAIEEAAQKGAQLVLLPEIWNSPYSND 133

Query: 132 SFPVYAEDIELGGDASPSTAMLSEVSRNLKVTVVGGSIPERCGDRLYNTCCVFGTDGKLK 191
           SFPVYAEDI+ GGDASPSTAMLSEV+  LK+T+VGGSIPERCGD+LYNTCCVFGTDGKLK
Sbjct: 134 SFPVYAEDIDAGGDASPSTAMLSEVASRLKITIVGGSIPERCGDKLYNTCCVFGTDGKLK 193

Query: 192 AKHRKIHLFDIDIPGKMTFKESKTLTAGESPTIVDTGCSGTSRADKYYFCTDVGRIGI-- 251
           AKHRKIHLFDIDIPGK+TF ESKTLTAGE+PT+VD               TDVGRIGI  
Sbjct: 194 AKHRKIHLFDIDIPGKITFMESKTLTAGETPTVVD---------------TDVGRIGIGI 253

Query: 252 ----------------GAHLLCYPGAFNMTTGPLHWELLQRARI----DFLNLPKRPESS 311
                           GAHL+CYPGAFNMTTGPLHWEL QRA      +FL +P      
Sbjct: 254 CYDIRFTELAMIYAARGAHLICYPGAFNMTTGPLHWELSQRASCSSSNEFLAVP------ 313

Query: 312 SSGTHLGFVKNRKLKQQTMPNLDLQL-LQRLYVATCAPARDTGSGYVAWGHSTLVGPFGE 371
                 G    RK    T+  +   +    LYVATC+PARD G+ YVAWGHSTLVGPFGE
Sbjct: 314 ----CAGLWIIRKNCPSTIEAVKTYVDSVTLYVATCSPARDPGADYVAWGHSTLVGPFGE 373

[Close]

BLAST of Spo03181.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RPT1_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_048130 PE=4 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 1.400e-124
Identity = 234/253 (92.49%), Postives = 235/253 (92.89%), Query Frame = 1


		  
Query: 1   MIAITQIQPLTTPHSLQNAYHQTKNLSFYSSQTSVVKLRIPNFSNPNKPPLSSRIAAMSV 60
           MIAITQIQPLTTPHSLQNAYHQTKNLSFYSSQTSVVKLRIPNFSNPNKPPLSSRIAAMSV
Sbjct: 1   MIAITQIQPLTTPHSLQNAYHQTKNLSFYSSQTSVVKLRIPNFSNPNKPPLSSRIAAMSV 60

Query: 61  ADQARVPLALPLPPPPVTKFKIALCQLSVTTDKLRNISHAREAIEEAANKGAQLILLPEI 120
           ADQARVPLALPLPPPPVTKFKIALCQLSVTTDKLRNISHAREAIEEAANKGAQLILLPEI
Sbjct: 61  ADQARVPLALPLPPPPVTKFKIALCQLSVTTDKLRNISHAREAIEEAANKGAQLILLPEI 120

Query: 121 WNSPYSNDSFPVYAEDIELGGDASPSTAMLSEVSRNLKVTVVGGSIPERCGDRLYNTCCV 180
           WNSPYSNDSFPVYAEDIELGGDASPSTAMLSEVSRNLKVTVVGGSIPERCGDRLYNTCCV
Sbjct: 121 WNSPYSNDSFPVYAEDIELGGDASPSTAMLSEVSRNLKVTVVGGSIPERCGDRLYNTCCV 180

Query: 181 FGTDGKLKAKHRKIHLFDIDIPGKMTFKESKTLTAGESPTIVDTGCSGTSRADKYYFCTD 240
           FGTDGKLKAKHRKIHLFDIDIPGKMTFKESKTLTAGESPTIVD               TD
Sbjct: 181 FGTDGKLKAKHRKIHLFDIDIPGKMTFKESKTLTAGESPTIVD---------------TD 235

Query: 241 VGRIGIGAHLLCY 254
           VGRIGIG   +CY
Sbjct: 241 VGRIGIG---ICY 235

[Close]

BLAST of Spo03181.1 vs. UniProtKB/TrEMBL
Match: A0A022QPD4_ERYGU (Uncharacterized protein (Fragment) OS=Erythranthe guttata GN=MIMGU_mgv1a022881mg PE=4 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 1.400e-119
Identity = 230/343 (67.06%), Postives = 261/343 (76.09%), Query Frame = 1


		  
Query: 51  LSSRIAAMSVADQARVPLALPLPPPPVTKFKIALCQLSVTTDKLRNISHAREAIEEAANK 110
           +SS   A+  +D+ARVP A+P P PPVTKFKIALCQLSVT DK RNI+HAR+ IEEAA K
Sbjct: 84  ISSATTAIMGSDEARVPPAIPPPTPPVTKFKIALCQLSVTADKERNIAHARQVIEEAAGK 143

Query: 111 GAQLILLPEIWNSPYSNDSFPVYAEDIELGGDASPSTAMLSEVSRNLKVTVVGGSIPERC 170
           GAQLILLPEIWNSPYSNDSFPVYAEDI+ GGDASPSTAMLSE+SR+L++T++GGSIPER 
Sbjct: 144 GAQLILLPEIWNSPYSNDSFPVYAEDIDAGGDASPSTAMLSELSRSLQITIIGGSIPERS 203

Query: 171 GDRLYNTCCVFGTDGKLKAKHRKIHLFDIDIPGKMTFKESKTLTAGESPTIVDTGCSGTS 230
           G++LYNTCCVFGTDGKL AKHRKIHLFDIDIPG+MTFKES TLTAGE+PTIVD       
Sbjct: 204 GEKLYNTCCVFGTDGKLMAKHRKIHLFDIDIPGQMTFKESNTLTAGETPTIVD------- 263

Query: 231 RADKYYFCTDVGRIGI------------------GAHLLCYPGAFNMTTGPLHWELLQRA 290
                   TDVGRIGI                  GAHLLCYPGAFNMTTGPLHWELLQRA
Sbjct: 264 --------TDVGRIGIGICYDIRFQELAAIYAARGAHLLCYPGAFNMTTGPLHWELLQRA 323

Query: 291 RIDFLNLPKRPESSS-SGTHLGFV----KNRKLKQQTMPNLDLQLLQRLYVATCAPARDT 350
            +    L    +SS  SGT    +       KL+   + + +L  ++ LYVATC+PARD 
Sbjct: 324 SLVLTCLLLDVQSSRLSGTLASLLILSKMTFKLQSGDITSQNLICVEALYVATCSPARDC 383

Query: 351 GSGYVAWGHSTLVGPFGEVLATTDHDETIVIAEIDYSVIEQRR 371
           GS YVAWGHSTLVGPFGEVLATT+HDE IVI+EIDYS+IEQRR
Sbjct: 384 GSSYVAWGHSTLVGPFGEVLATTEHDEAIVISEIDYSLIEQRR 411

[Close]

BLAST of Spo03181.1 vs. UniProtKB/TrEMBL
Match: A0A0B0MNB7_GOSAR (Omega-amidase NIT2 OS=Gossypium arboreum GN=F383_04107 PE=4 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 4.200e-116
Identity = 234/385 (60.78%), Postives = 269/385 (69.87%), Query Frame = 1


		  
Query: 12  TPHSLQNAYHQTKNLS---FYSSQTSVVKLRIPNFSNPNKPPLSSRIAAMSVADQARVPL 71
           +P S    YH +K +S   F+ S T               P  +S +A++S ++QAR P 
Sbjct: 14  SPISRTLLYHSSKPISLSPFFLSNTPKNLFNQRYQKLQFSPNSTSIMASLSKSEQAREPP 73

Query: 72  ALPLPPPPVTKFKIALCQLSVTTDKLRNISHAREAIEEAANKGAQLILLPEIWNSPYSND 131
           ALPLP PP++KFKI LCQLSVT DK RNI HAR+AIEEAA KGAQL+LLPEIWNSPYSND
Sbjct: 74  ALPLPSPPLSKFKIGLCQLSVTPDKERNIKHARKAIEEAAQKGAQLVLLPEIWNSPYSND 133

Query: 132 SFPVYAEDIELGGDASPSTAMLSEVSRNLKVTVVGGSIPERCGDRLYNTCCVFGTDGKLK 191
           SFPVYAEDI+ GGDASPSTAMLSEV+  LK+T+VGGSIPERCGD+LYNTCCVFGTDGKLK
Sbjct: 134 SFPVYAEDIDAGGDASPSTAMLSEVASRLKITIVGGSIPERCGDKLYNTCCVFGTDGKLK 193

Query: 192 AKHRKIHLFDIDIPGKMTFKESKTLTAGESPTIVDTGCSGTSRADKYYFCTDVGRIGI-- 251
           AKHRKIHLFDIDIPGK+TF ESKTLTAGE+PT+VD               TDVGRIGI  
Sbjct: 194 AKHRKIHLFDIDIPGKITFMESKTLTAGETPTVVD---------------TDVGRIGIGI 253

Query: 252 ----------------GAHLLCYPGAFNMTTGPLHWELLQRARI----DFLNLPKRPESS 311
                           GAHL+CYPGAFNMTTGPLHWEL QRA      +FL +P      
Sbjct: 254 CYDIRFTELAMIYAARGAHLICYPGAFNMTTGPLHWELSQRASCSSSNEFLAVP------ 313

Query: 312 SSGTHLGFVKNRKLKQQTMPNLDLQL-LQRLYVATCAPARDTGSGYVAWGHSTLVGPFGE 371
                 G    RK    T+  +   +    LYVATC+PARD G+ YVAWGHSTLVGPFGE
Sbjct: 314 ----CAGLWIIRKNCPSTIEAVKTYVDSVTLYVATCSPARDPGADYVAWGHSTLVGPFGE 373

[Close]

BLAST of Spo03181.1 vs. UniProtKB/TrEMBL
Match: A0A0J8B4J3_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_002160 PE=4 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 7.900e-115
Identity = 228/346 (65.90%), Postives = 253/346 (73.12%), Query Frame = 1


		  
Query: 25  NLSFYSSQTSVVKLRIPNFSNPNKPPLSSRIAAMSVADQARVPLALPLPPPPVTKFKIAL 84
           NLS+ SS+T  V  R  N S   K  L ++I+AMS + QARVP  LPLPPPPVTKFKIAL
Sbjct: 12  NLSYSSSETFDVSFRKRNPSISRKSLLFTKISAMSTSHQARVPPPLPLPPPPVTKFKIAL 71

Query: 85  CQLSVTTDKLRNISHAREAIEEAANKGAQLILLPEIWNSPYSNDSFPVYAEDIELGGDAS 144
           CQL VT DK RNI HAREAIEEAANKGAQL+LLPEIWNSPYSN SFP+YAEDIELGGDAS
Sbjct: 72  CQLLVTADKSRNIFHAREAIEEAANKGAQLVLLPEIWNSPYSNVSFPIYAEDIELGGDAS 131

Query: 145 PSTAMLSEVSRNLKVTVVGGSIPERCGDRLYNTCCVFGTDGKLKAKHRKIHLFDIDIPGK 204
           PSTAMLSEVS+NLK+T+VGGSIPERCGDRLYNTCCVFGTDGKLKAKHRKIHLFDIDIPG+
Sbjct: 132 PSTAMLSEVSQNLKITIVGGSIPERCGDRLYNTCCVFGTDGKLKAKHRKIHLFDIDIPGQ 191

Query: 205 MTFKESKTLTAGESPTIVDTGCSGTSRADKYYFCTDVGRIGIGAHLLCYPGAFNMTTGPL 264
           MTFKES TLTAGE+PTIVDT               DVGRIGIG   +CY   F       
Sbjct: 192 MTFKESNTLTAGENPTIVDT---------------DVGRIGIG---ICYDIRFQELAA-- 251

Query: 265 HWELLQRARIDFLNLPKRPESSSSGTHLGFVKNRKLKQQTMPNLDLQLLQRLYVATCAPA 324
              +        L  P     ++   H   ++  +               +LYVATC+PA
Sbjct: 252 ---IYAARGAHLLCYPGAFNMTTGPLHWELLQRARAAD-----------NQLYVATCSPA 311

Query: 325 RDTGSGYVAWGHSTLVGPFGEVLATTDHDETIVIAEIDYSVIEQRR 371
           RDTGSGYVAWGHSTLVGPFGEVLATTDH+ETIVI+EIDYS+IEQRR
Sbjct: 312 RDTGSGYVAWGHSTLVGPFGEVLATTDHEETIVISEIDYSLIEQRR 323

[Close]

BLAST of Spo03181.1 vs. UniProtKB/TrEMBL
Match: A0A0D2QIK0_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_001G021100 PE=4 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 6.300e-104
Identity = 203/323 (62.85%), Postives = 236/323 (73.07%), Query Frame = 1


		  
Query: 49  PPLSSRIAAMSVADQARVPLALPLPPPPVTKFKIALCQLSVTTDKLRNISHAREAIEEAA 108
           P  +S +A++S ++QAR P ALPLP PP+TKFKI LCQLSVT DK RNI HAR+AIEEAA
Sbjct: 54  PNSTSIMASLSKSEQAREPPALPLPSPPLTKFKIGLCQLSVTPDKERNIKHARKAIEEAA 113

Query: 109 NKGAQLILLPEIWNSPYSNDSFPVYAEDIELGGDASPSTAMLSEVSRNLKVTVVGGSIPE 168
            KGAQL+LLPEIWNSPYSNDSFPVYAEDI  GGDASPSTAMLSEV+  LK+T+VGGSIPE
Sbjct: 114 QKGAQLVLLPEIWNSPYSNDSFPVYAEDINAGGDASPSTAMLSEVASRLKITIVGGSIPE 173

Query: 169 RCGDRLYNTCCVFGTDGKLKAKHRKIHLFDIDIPGKMTFKESKTLTAGESPTIVDTGCSG 228
           RCGD+LYNTCCVFGTDGKLKAKHRKIHLFDIDIPGK+TF ESKTLTAGE+PT+VDT    
Sbjct: 174 RCGDKLYNTCCVFGTDGKLKAKHRKIHLFDIDIPGKITFMESKTLTAGETPTVVDT---- 233

Query: 229 TSRADKYYFCTDVGRIGIGAHLLCYPGAFNMTTGPLHWELLQRAR-IDFLNLPKRPESSS 288
                      DVGRIGIG   +CY   F          ++  AR    +  P     ++
Sbjct: 234 -----------DVGRIGIG---ICYDIRFTELA------MIYAARGAHLICYPGAFNMTT 293

Query: 289 SGTHLGFVKNRKLKQQTMPNLDLQLLQRLYVATCAPARDTGSGYVAWGHSTLVGPFGEVL 348
              H    +  +               +LYVATC+PARD G+GYVAWGHSTLVGPFGEVL
Sbjct: 294 GPLHWELSQRARAMD-----------NQLYVATCSPARDPGAGYVAWGHSTLVGPFGEVL 341

Query: 349 ATTDHDETIVIAEIDYSVIEQRR 371
           A+T+H+E I+IAEIDYS++EQRR
Sbjct: 354 ASTEHEEDIIIAEIDYSILEQRR 341

[Close]

BLAST of Spo03181.1 vs. ExPASy Swiss-Prot
Match: NILP3_ARATH (Omega-amidase, chloroplastic OS=Arabidopsis thaliana GN=NLP3 PE=1 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 5.600e-106
Identity = 203/334 (60.78%), Postives = 244/334 (73.05%), Query Frame = 1


		  
Query: 38  LRIPNFSNPNKPPLSSRIAAMSVADQARVPLALPLPPPPVTKFKIALCQLSVTTDKLRNI 97
           L++P+ S      +SS +A+    +QARVP ALPLP PP+TKF I LCQLSVT+DK RNI
Sbjct: 46  LKLPSSSTSALRSISSSMASSFNPEQARVPSALPLPAPPLTKFNIGLCQLSVTSDKKRNI 105

Query: 98  SHAREAIEEAANKGAQLILLPEIWNSPYSNDSFPVYAEDIELGGDASPSTAMLSEVSRNL 157
           SHA++AIEEAA+KGA+L+LLPEIWNSPYSNDSFPVYAE+I+ GGDASPSTAMLSEVS+ L
Sbjct: 106 SHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGGDASPSTAMLSEVSKRL 165

Query: 158 KVTVVGGSIPERCGDRLYNTCCVFGTDGKLKAKHRKIHLFDIDIPGKMTFKESKTLTAGE 217
           K+T++GGSIPER GDRLYNTCCVFG+DG+LKAKHRKIHLFDIDIPGK+TF ESKTLTAGE
Sbjct: 166 KITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFMESKTLTAGE 225

Query: 218 SPTIVDTGCSGTSRADKYYFCTDVGRIGIGAHLLCYPGAFNMTTGPLHWELLQRAR-IDF 277
           +PTIVDT               DVGRIGIG   +CY   F          ++  AR    
Sbjct: 226 TPTIVDT---------------DVGRIGIG---ICYDIRFQELA------MIYAARGAHL 285

Query: 278 LNLPKRPESSSSGTHLGFVKNRKLKQQTMPNLDLQLLQRLYVATCAPARDTGSGYVAWGH 337
           L  P     ++   H   ++  +               +LYVATC+PARD+G+GY AWGH
Sbjct: 286 LCYPGAFNMTTGPLHWELLQRARATD-----------NQLYVATCSPARDSGAGYTAWGH 344

Query: 338 STLVGPFGEVLATTDHDETIVIAEIDYSVIEQRR 371
           STLVGPFGEVLATT+H+E I+IAEIDYS++EQRR
Sbjct: 346 STLVGPFGEVLATTEHEEAIIIAEIDYSILEQRR 344

[Close]

BLAST of Spo03181.1 vs. ExPASy Swiss-Prot
Match: NIT2_HUMAN (Omega-amidase NIT2 OS=Homo sapiens GN=NIT2 PE=1 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 9.700e-50
Identity = 119/305 (39.02%), Postives = 168/305 (55.08%), Query Frame = 1


		  
Query: 77  VTKFKIALCQLSVTTDKLRNISHAREAIEEAANKGAQLILLPEIWNSPYSNDSFPVYAED 136
           +T F++AL QL +++ K  N++ A   I EAA +GA+++ LPE +NSPY    FP YAE 
Sbjct: 1   MTSFRLALIQLQISSIKSDNVTRACSFIREAATQGAKIVSLPECFNSPYGAKYFPEYAEK 60

Query: 137 IELGGDASPSTAMLSEVSRNLKVTVVGGSIPERCGDRLYNTCCVFGTDGKLKAKHRKIHL 196
           I        ST  LSEV++   + ++GGSIPE    +LYNTC VFG DG L AK+RKIHL
Sbjct: 61  IP-----GESTQKLSEVAKECSIYLIGGSIPEEDAGKLYNTCAVFGPDGTLLAKYRKIHL 120

Query: 197 FDIDIPGKMTFKESKTLTAGESPTIVDTGCSGTSRADKYYFCTDVGRIGIGAHLLCYPGA 256
           FDID+PGK+TF+ESKTL+ G+S +  DT            +C    R+G+G   +CY   
Sbjct: 121 FDIDVPGKITFQESKTLSPGDSFSTFDTP-----------YC----RVGLG---ICYDMR 180

Query: 257 FNMTTGPLHWELLQRARIDFLNLPKRPESSSSGTHLGFVKNRKLKQQTMPNLDLQLLQRL 316
           F         ++  +     L  P     ++   H   ++  +               ++
Sbjct: 181 FAELA-----QIYAQRGCQLLVYPGAFNLTTGPAHWELLQRSRAVDN-----------QV 240

Query: 317 YVATCAPARDTGSGYVAWGHSTLVGPFGEVLATTDHDETIVIAEIDYSVIEQRRQCISNF 376
           YVAT +PARD  + YVAWGHST+V P+GEVLA    +E IV ++ID   + + RQ I  F
Sbjct: 241 YVATASPARDDKASYVAWGHSTVVNPWGEVLAKAGTEEAIVYSDIDLKKLAEIRQQIPVF 266

Query: 377 NNLAS 382
               S
Sbjct: 301 RQKRS 266

[Close]

BLAST of Spo03181.1 vs. ExPASy Swiss-Prot
Match: NIT2_BOVIN (Omega-amidase NIT2 OS=Bos taurus GN=NIT2 PE=2 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 1.700e-49
Identity = 121/309 (39.16%), Postives = 173/309 (55.99%), Query Frame = 1


		  
Query: 80  FKIALCQLSVTTDKLRNISHAREAIEEAANKGAQLILLPEIWNSPYSNDSFPVYAEDIEL 139
           F++AL QL V++ K  N++ A   I EA+ +GAQ++ LPE +NSPY    FP YAE I  
Sbjct: 4   FRLALIQLQVSSIKSENLTRACGLIREASKQGAQIVSLPECFNSPYGTKYFPDYAEKIP- 63

Query: 140 GGDASPSTAMLSEVSRNLKVTVVGGSIPERCGDRLYNTCCVFGTDGKLKAKHRKIHLFDI 199
            GD   ST  LSEV++   + V+GGSIPE+   +LYNTC VFG DG L  KHRK+HLFDI
Sbjct: 64  -GD---STQKLSEVAKECSMYVIGGSIPEKDAGKLYNTCAVFGPDGTLLVKHRKLHLFDI 123

Query: 200 DIPGKMTFKESKTLTAGESPTIVDTGCSGTSRADKYYFCTDVGRIGIGAHLLCYPGAFNM 259
           D+PGK+TF+ES+TL+ G+S ++ DT            +C    R+G+    +CY   F  
Sbjct: 124 DVPGKITFQESETLSPGDSFSLFDTP-----------YC----RVGL---GICYDIRFAE 183

Query: 260 TTGPLHWELLQRARIDFLNLPKRPESSSSGTHLGFVKNRKLKQQTMPNLDLQLLQRLYVA 319
                  ++  +     L  P     ++   H   ++  +               ++YVA
Sbjct: 184 LA-----QIYAQRGCQLLVYPGAFNLTTGPAHWELLQRGRAVDN-----------QVYVA 243

Query: 320 TCAPARDTGSGYVAWGHSTLVGPFGEVLATTDHDETIVIAEIDYSVIEQRRQCISNFNNL 379
           T +PARD  + YVAWGHST+V P+GEVLA    +ETIV A+ID   + + RQ I  F+  
Sbjct: 244 TASPARDEKASYVAWGHSTVVNPWGEVLAKAGTEETIVYADIDLKKLAEIRQQIPIFSQK 273

Query: 380 ASSQLQLSS 389
            S   ++ +
Sbjct: 304 RSDLYEVEA 273

[Close]

BLAST of Spo03181.1 vs. ExPASy Swiss-Prot
Match: NIT2A_XENLA (Omega-amidase NIT2-A OS=Xenopus laevis GN=nit2a PE=2 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 1.700e-49
Identity = 117/297 (39.39%), Postives = 168/297 (56.57%), Query Frame = 1


		  
Query: 77  VTKFKIALCQLSVTTDKLRNISHAREAIEEAANKGAQLILLPEIWNSPYSNDSFPVYAED 136
           + KFK++L Q  V+  K  N++ A + I+EAA KGAQ++ LPE +NSPY    FP YAE 
Sbjct: 1   MAKFKLSLVQFLVSPVKSDNLNRACKLIKEAAQKGAQIVALPECFNSPYGTTYFPEYAEK 60

Query: 137 IELGGDASPSTAMLSEVSRNLKVTVVGGSIPERCGDRLYNTCCVFGTDGKLKAKHRKIHL 196
           I        ST +LS+V++   + ++GGSIPE    +LYNTC VFG DG L  KHRKIHL
Sbjct: 61  IP-----GESTELLSQVAKECGIYLIGGSIPEEDCGKLYNTCAVFGPDGTLLVKHRKIHL 120

Query: 197 FDIDIPGKMTFKESKTLTAGESPTIVDTGCSGTSRADKYYFCTDVGRIGIGAHLLCYPGA 256
           FDID+PGK+ F+ES+TL+ G+S ++ DT            +C    ++G+G   +CY   
Sbjct: 121 FDIDVPGKIRFQESETLSPGDSFSVFDTP-----------YC----KVGVG---ICYDIR 180

Query: 257 FNMTTGPLHWELLQRARIDFLNLPKRPESSSSGTHLGFVKNRKLKQQTMPNLDLQLLQRL 316
           F         ++        L  P     ++   H   ++  +        LD Q+    
Sbjct: 181 FAELA-----QIYANKGCQLLVYPGAFNMTTGPAHWELLQRARA-------LDNQV---- 240

Query: 317 YVATCAPARDTGSGYVAWGHSTLVGPFGEVLATTDHDETIVIAEIDYSVIEQRRQCI 374
           YVAT +PARD  + YVAWGHST+V P+GEV+A    +ET++ AEID   + + R+ I
Sbjct: 241 YVATASPARDEKASYVAWGHSTIVSPWGEVVAKAGSEETVLSAEIDLQYLAEIREQI 258

[Close]

BLAST of Spo03181.1 vs. ExPASy Swiss-Prot
Match: NIT2_XENTR (Omega-amidase NIT2 OS=Xenopus tropicalis GN=nit2 PE=2 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 1.700e-49
Identity = 115/297 (38.72%), Postives = 168/297 (56.57%), Query Frame = 1


		  
Query: 77  VTKFKIALCQLSVTTDKLRNISHAREAIEEAANKGAQLILLPEIWNSPYSNDSFPVYAED 136
           + KF+++L Q  V+  K  N++ A + I+EAA KGAQ++ LPE +NSPY    FP YAE 
Sbjct: 1   MAKFRLSLVQFLVSPVKSENLNRACKLIKEAAQKGAQIVALPECFNSPYGTKYFPEYAEK 60

Query: 137 IELGGDASPSTAMLSEVSRNLKVTVVGGSIPERCGDRLYNTCCVFGTDGKLKAKHRKIHL 196
           I        ST  LS+V++   + ++GGSIPE    +LYNTC VFG DG L  KHRKIHL
Sbjct: 61  IP-----GESTERLSQVAKECGIYLIGGSIPEEDSGKLYNTCAVFGPDGTLLVKHRKIHL 120

Query: 197 FDIDIPGKMTFKESKTLTAGESPTIVDTGCSGTSRADKYYFCTDVGRIGIGAHLLCYPGA 256
           FDID+PGK+ F+ES+TL+ G+S ++ +T            +C    ++G+G   +CY   
Sbjct: 121 FDIDVPGKIRFQESETLSPGDSFSVFETP-----------YC----KVGVG---ICYDIR 180

Query: 257 FNMTTGPLHWELLQRARIDFLNLPKRPESSSSGTHLGFVKNRKLKQQTMPNLDLQLLQRL 316
           F         +L  +     L  P     ++   H   ++  +        LD Q+    
Sbjct: 181 FAELA-----QLYSKKGCQLLVYPGAFNMTTGPAHWELLQRARA-------LDNQV---- 240

Query: 317 YVATCAPARDTGSGYVAWGHSTLVGPFGEVLATTDHDETIVIAEIDYSVIEQRRQCI 374
           YVAT +PARD  + YVAWGHST+V P+GEV+A    +ET++ A+ID   + + R+ I
Sbjct: 241 YVATASPARDEKASYVAWGHSTIVSPWGEVIAKAGSEETVISADIDLEYLAEIREQI 258

[Close]

BLAST of Spo03181.1 vs. TAIR (Arabidopsis)
Match: AT5G12040.1 (Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase family protein)

HSP 1 Score: 386.0 bits (990), Expect = 3.200e-107
Identity = 203/334 (60.78%), Postives = 244/334 (73.05%), Query Frame = 1


		  
Query: 38  LRIPNFSNPNKPPLSSRIAAMSVADQARVPLALPLPPPPVTKFKIALCQLSVTTDKLRNI 97
           L++P+ S      +SS +A+    +QARVP ALPLP PP+TKF I LCQLSVT+DK RNI
Sbjct: 46  LKLPSSSTSALRSISSSMASSFNPEQARVPSALPLPAPPLTKFNIGLCQLSVTSDKKRNI 105

Query: 98  SHAREAIEEAANKGAQLILLPEIWNSPYSNDSFPVYAEDIELGGDASPSTAMLSEVSRNL 157
           SHA++AIEEAA+KGA+L+LLPEIWNSPYSNDSFPVYAE+I+ GGDASPSTAMLSEVS+ L
Sbjct: 106 SHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGGDASPSTAMLSEVSKRL 165

Query: 158 KVTVVGGSIPERCGDRLYNTCCVFGTDGKLKAKHRKIHLFDIDIPGKMTFKESKTLTAGE 217
           K+T++GGSIPER GDRLYNTCCVFG+DG+LKAKHRKIHLFDIDIPGK+TF ESKTLTAGE
Sbjct: 166 KITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFMESKTLTAGE 225

Query: 218 SPTIVDTGCSGTSRADKYYFCTDVGRIGIGAHLLCYPGAFNMTTGPLHWELLQRAR-IDF 277
           +PTIVDT               DVGRIGIG   +CY   F          ++  AR    
Sbjct: 226 TPTIVDT---------------DVGRIGIG---ICYDIRFQELA------MIYAARGAHL 285

Query: 278 LNLPKRPESSSSGTHLGFVKNRKLKQQTMPNLDLQLLQRLYVATCAPARDTGSGYVAWGH 337
           L  P     ++   H   ++  +               +LYVATC+PARD+G+GY AWGH
Sbjct: 286 LCYPGAFNMTTGPLHWELLQRARATD-----------NQLYVATCSPARDSGAGYTAWGH 344

Query: 338 STLVGPFGEVLATTDHDETIVIAEIDYSVIEQRR 371
           STLVGPFGEVLATT+H+E I+IAEIDYS++EQRR
Sbjct: 346 STLVGPFGEVLATTEHEEAIIIAEIDYSILEQRR 344

[Close]

BLAST of Spo03181.1 vs. TAIR (Arabidopsis)
Match: AT4G08790.1 (Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase family protein)

HSP 1 Score: 80.1 bits (196), Expect = 3.700e-15
Identity = 69/235 (29.36%), Postives = 105/235 (44.68%), Query Frame = 1


		  
Query: 48  KPPLSSRIAAMSVADQARVPLALPLPPPPVTKFKIALCQLSVTTDKLRNISHAREAIEEA 107
           KP L +RI   S     ++PL +        +  +A  Q++   D + N +     ++EA
Sbjct: 12  KPSLFTRITLSS-----QIPLTMATTVNKTVR--VAAAQMTSVNDLMTNFATCSRLVQEA 71

Query: 108 ANKGAQLILLPEIWNSPYSNDSFPV-YAEDIELGGDASPSTAMLSEVSRNLKVTVVGGSI 167
           A  GA+LI  PE ++     +   V  AE ++      P       ++R+  + +  G  
Sbjct: 72  ALAGAKLICFPENFSFVGDKEGESVKIAEPLD-----GPVMERYCSLARDSNIWLSLGGF 131

Query: 168 PERCGD-RLYNTCCVFGTDGKLKAKHRKIHLFDIDIPGKMTFKESKTLTAGESPTIVDT- 227
            ER  D  L NT  V    G ++  ++K+HLFD+D+PG  ++KES     G     VD+ 
Sbjct: 132 QERFDDTHLCNTHVVIDDAGMIRDTYQKMHLFDVDVPGGSSYKESSFTVPGTKIVSVDSP 191

Query: 228 -GCSGTS-----RADKYYFCTDVGRIGIGAHLLCYPGAFNMTTGPLHWELLQRAR 274
            G  G +     R  K Y      R    A +L  P AF   TG  HWE+L RAR
Sbjct: 192 VGRLGLTVCYDLRFPKIY---QQLRFEQKAQVLLVPSAFTKVTGEAHWEILLRAR 231

[Close]

BLAST of Spo03181.1 vs. TAIR (Arabidopsis)
Match: AT3G44310.1 (nitrilase 1)

HSP 1 Score: 52.4 bits (124), Expect = 8.300e-7
Identity = 51/155 (32.90%), Postives = 69/155 (44.52%), Query Frame = 1


		  
Query: 59  SVADQARVPLALPLPP-PPVTKFKIALCQLS-VTTDKLRNISHAREAIEEAANKGAQLIL 118
           S  D + V  A P     P T  ++ + Q S V  D    I  A + I EAA+KGA+L+L
Sbjct: 3   STKDMSTVQNATPFNGVAPSTTVRVTIVQSSTVYNDTPATIDKAEKYIVEAASKGAELVL 62

Query: 119 LPEIWNSPY----------------SNDSFPVY-AEDIELGGDASPSTAMLSEVSRNLKV 178
            PE +   Y                  D F  Y A  I + G   P  A L++V+R   V
Sbjct: 63  FPEGFIGGYPRGFRFGLAVGVHNEEGRDEFRKYHASAIHVPG---PEVARLADVARKNHV 122

Query: 179 TVVGGSIPERCGDRLYNTCCVFGTDGKLKAKHRKI 195
            +V G+I E+ G  LY T   F   G+   KHRK+
Sbjct: 123 YLVMGAI-EKEGYTLYCTVLFFSPQGQFLGKHRKL 153

The following BLAST results are available for this feature:

BLAST of Spo03181.1 vs. NCBI nr
BLAST of Spo03181.1 vs. UniProtKB/TrEMBL
BLAST of Spo03181.1 vs. ExPASy Swiss-Prot
BLAST of Spo03181.1 vs. TAIR (Arabidopsis)

BLAST of Spo03181.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902227977\|gb\|KNA20904.1\|	2.1e-124	92.4	hypothetical protein SOVF_0481...	[more]
gi\|604315785\|gb\|EYU28350.1\|	2.0e-119	67.0	hypothetical protein MIMGU_mgv...	[more]
gi\|698501289\|ref\|XP_009796352.1\|	1.9e-117	65.7	PREDICTED: omega-amidase,chlor...	[more]
gi\|731372813\|ref\|XP_010666455.1\|	5.4e-117	64.5	PREDICTED: omega-amidase,chlor...	[more]
gi\|728815404\|gb\|KHG01832.1\|	6.0e-116	60.7	Omega-amidase NIT2 [Gossypium ...	[more]

BLAST of Spo03181.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RPT1_SPIOL	1.4e-124	92.4	Uncharacterized protein OS=Spi...	[more]
A0A022QPD4_ERYGU	1.4e-119	67.0	Uncharacterized protein (Fragm...	[more]
A0A0B0MNB7_GOSAR	4.2e-116	60.7	Omega-amidase NIT2 OS=Gossypiu...	[more]
A0A0J8B4J3_BETVU	7.9e-115	65.9	Uncharacterized protein OS=Bet...	[more]
A0A0D2QIK0_GOSRA	6.3e-104	62.8	Uncharacterized protein OS=Gos...	[more]

BLAST of Spo03181.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
NILP3_ARATH	5.6e-106	60.7	Omega-amidase, chloroplastic O...	[more]
NIT2_HUMAN	9.7e-50	39.0	Omega-amidase NIT2 OS=Homo sap...	[more]
NIT2_BOVIN	1.7e-49	39.1	Omega-amidase NIT2 OS=Bos taur...	[more]
NIT2A_XENLA	1.7e-49	39.3	Omega-amidase NIT2-A OS=Xenopu...	[more]
NIT2_XENTR	1.7e-49	38.7	Omega-amidase NIT2 OS=Xenopus ...	[more]

BLAST of Spo03181.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 3


Match Name	E-value	Identity	Description
AT5G12040.1	3.2e-107	60.7	Nitrilase/cyanide hydratase an...	[more]
AT4G08790.1	3.7e-15	29.3	Nitrilase/cyanide hydratase an...	[more]
AT3G44310.1	8.3e-7	32.9	nitrilase 1	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003010	Carbon-nitrogen hydrolase	GENE3D	3.60.110.10		coord: 311..376 score: 4.9E-61coord: 79..267 score: 4.9
IPR003010	Carbon-nitrogen hydrolase	PFAM	PF00795	CN_hydrolase	coord: 317..370 score: 1.2E-5coord: 81..273 score: 9.4
IPR003010	Carbon-nitrogen hydrolase	PROFILE	PS50263	CN_HYDROLASE	coord: 80..388 score: 28
IPR003010	Carbon-nitrogen hydrolase	unknown	SSF56317	Carbon-nitrogen hydrolase	coord: 318..378 score: 3.84E-54coord: 73..280 score: 3.84
None	No IPR available	PANTHER	PTHR23088	NITRILASE-RELATED	coord: 64..224 score: 1.3E-108coord: 309..390 score: 1.3E-108coord: 240..264 score: 1.3E
None	No IPR available	PANTHER	PTHR23088:SF20	SUBFAMILY NOT NAMED	coord: 309..390 score: 1.3E-108coord: 64..224 score: 1.3E-108coord: 240..264 score: 1.3E

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006522	alanine metabolic process
biological_process	GO:0006531	aspartate metabolic process
biological_process	GO:0006108	malate metabolic process
biological_process	GO:0006107	oxaloacetate metabolic process
biological_process	GO:0008152	metabolic process
biological_process	GO:0006626	protein targeting to mitochondrion
biological_process	GO:0006807	nitrogen compound metabolic process
cellular_component	GO:0009570	chloroplast stroma
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0050152	omega-amidase activity
molecular_function	GO:0016740	transferase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0016810	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo08106	0.70	Barchart \| Table
Spo13240	0.70	Barchart \| Table
Spo24871	0.69	Barchart \| Table
Spo25846	0.69	Barchart \| Table
Spo13374	0.69	Barchart \| Table
Spo27291	0.68	Barchart \| Table
Spo18150	0.68	Barchart \| Table
Spo05733	0.67	Barchart \| Table
Spo18296	0.66	Barchart \| Table
Spo23445	0.66	Barchart \| Table
Spo15025	0.66	Barchart \| Table
Spo26235	0.66	Barchart \| Table
Spo02519	0.65	Barchart \| Table