Spo03302 (gene)

Overview
NameSpo03302
Typegene
OrganismSpinacia oleracea (Spinach)
DescriptionPutative thiol-disulphide oxidoreductase DCC
LocationSpoScf_00897 : 242725 .. 243943 (+)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATAGGGTTTGCTAGGCTGCTAGCTCCGATACCATGACAAGTTTATTAGGGTTATGATTTTCCTTGAGTTTGTATTGCATTGGAATTGGTATTATATACTACAGCATTAGCTACGGGCTTTCCTAGACTTAGTATTACTAAATACAAGCTAAGCCTATTAAAGCCCAACTACAAAATACACAATATATATATATATATATATATATAAGCCTTAATATTTTCCCACTTGTTACCCCCACCATCACATGCTCACCAAAGTTTCTTAACTACCATGCAAATATTAAGCGTAAAGATCATTTAGGAACGGAGGTAGTATATGTTTGTATTACATGCTTATGTCTCTAACAAATGAAAAAGAATCTTTGCTTTGTGCATATGCTAATAGTATGATCATCTGATATTTACATGGTTTCGGGTAGAAAAAATCTGTAGATGTAAGATTGATTGTTTTCTATTGGGTATTATTGCAGGAAAATCAGATATGAAGCTCTACAAAGTGAAGCAGGAAAGAATTTGTTAAGGAGATCAGGAAGAGCTCCTGATGATATATCAAGTGTTGTCCTTGTTGAAAAAGAGAGGTACGAGTTTGTTTTGATAATATACGAGTTTCTTGGTTTATTGATCTAGAGTCGAACAAGAAAAAAAACTCACTGCTCCATGCTCTAGAGTCCGTTATCTTTTGGGAAGCAATCAGTGATTATTAGTGACATTTGTTTTTCTTTGGTACCCACTGATATGTAATACACATAATTCACAAATTTCTTGATTTTTCCTGTTTCCTACTGCATCTCATCAACTCACTGCACCACAGAAGACTGTGTGATCCTACTTAGTTGGATTTCCCTCTAAAACCAACTGTCTGTGCATGTTTCTGCAGATCTTACATCAAGTCTGAAGCTGTATTAAAGATCATGGAATACATAAATTTACCATTCCCACAGATGGCATTCTTCCTGAAACTTTTACCTTTGTGAGTAATTTAATATTTTGCTGTTTAAGAAATTATCCCATCTTTGCAGCTGGACAAGTCTCTAATTTGACCTTTTAACAATTTTTTCCTGTAACTACCACAGGCTCGTTCGTGATTTTGCATACGACAATTTAGCAAACAACCGTTACAACATTTTTGGTAAGTCAGAGTCGTGCGAGATTTATTTAGATCAGTGAATGTCATCAATTTCTTCCTTGAGAATTTCGTACATCTAATCCTGATACAT

mRNA sequence

ATGATAGGGAAAATCAGATATGAAGCTCTACAAAGTGAAGCAGGAAAGAATTTGTTAAGGAGATCAGGAAGAGCTCCTGATGATATATCAAGTGTTGTCCTTGTTGAAAAAGAGAGATCTTACATCAAGTCTGAAGCTGTATTAAAGATCATGGAATACATAAATTTACCATTCCCACAGATGGCATTCTTCCTGAAACTTTTACCTTTGCTCGTTCGTGATTTTGCATACGACAATTTAGCAAACAACCGTTACAACATTTTTGGTAAGTCAGAGTCGTGCGAGATTTATTTAGATCAGTGAATGTCATCAATTTCTTCCTTGAGAATTTCGTACATCTAATCCTGATACAT

Coding sequence (CDS)

ATGATAGGGAAAATCAGATATGAAGCTCTACAAAGTGAAGCAGGAAAGAATTTGTTAAGGAGATCAGGAAGAGCTCCTGATGATATATCAAGTGTTGTCCTTGTTGAAAAAGAGAGATCTTACATCAAGTCTGAAGCTGTATTAAAGATCATGGAATACATAAATTTACCATTCCCACAGATGGCATTCTTCCTGAAACTTTTACCTTTGCTCGTTCGTGATTTTGCATACGACAATTTAGCAAACAACCGTTACAACATTTTTGGTAAGTCAGAGTCGTGCGAGATTTATTTAGATCAGTGA

Protein sequence

MIGKIRYEALQSEAGKNLLRRSGRAPDDISSVVLVEKERSYIKSEAVLKIMEYINLPFPQMAFFLKLLPLLVRDFAYDNLANNRYNIFGKSESCEIYLDQ
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo03302.1Spo03302.1mRNA


Homology
BLAST of Spo03302.1 vs. NCBI nr
Match: gi|902228833|gb|KNA21277.1| (hypothetical protein SOVF_044450 [Spinacia oleracea])

HSP 1 Score: 191.8 bits (486), Expect = 5.700e-46
Identity = 97/97 (100.00%), Postives = 97/97 (100.00%), Query Frame = 1

		  

Query: 4   KIRYEALQSEAGKNLLRRSGRAPDDISSVVLVEKERSYIKSEAVLKIMEYINLPFPQMAF 63
           KIRYEALQSEAGKNLLRRSGRAPDDISSVVLVEKERSYIKSEAVLKIMEYINLPFPQMAF
Sbjct: 79  KIRYEALQSEAGKNLLRRSGRAPDDISSVVLVEKERSYIKSEAVLKIMEYINLPFPQMAF 138

Query: 64  FLKLLPLLVRDFAYDNLANNRYNIFGKSESCEIYLDQ 101
           FLKLLPLLVRDFAYDNLANNRYNIFGKSESCEIYLDQ
Sbjct: 139 FLKLLPLLVRDFAYDNLANNRYNIFGKSESCEIYLDQ 175

BLAST of Spo03302.1 vs. NCBI nr
Match: gi|731359191|ref|XP_010691152.1| (PREDICTED: DCC family protein At1g52590, chloroplastic [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 172.9 bits (437), Expect = 2.700e-40
Identity = 85/95 (89.47%), Postives = 91/95 (95.79%), Query Frame = 1

		  

Query: 5   IRYEALQSEAGKNLLRRSGRAPDDISSVVLVEKERSYIKSEAVLKIMEYINLPFPQMAFF 64
           IRYEALQSEAGK LLRRSGR+PDDISSVVLVEKERSYIKSEAVLKIMEYI+LPFPQ+AFF
Sbjct: 79  IRYEALQSEAGKKLLRRSGRSPDDISSVVLVEKERSYIKSEAVLKIMEYIDLPFPQLAFF 138

Query: 65  LKLLPLLVRDFAYDNLANNRYNIFGKSESCEIYLD 100
           L+ LPL +RDFAYDNLANNRY IFGKSESCEIY+D
Sbjct: 139 LQFLPLFIRDFAYDNLANNRYTIFGKSESCEIYVD 173

BLAST of Spo03302.1 vs. NCBI nr
Match: gi|802603672|ref|XP_012073206.1| (PREDICTED: DCC family protein At1g52590, chloroplastic [Jatropha curcas])

HSP 1 Score: 170.2 bits (430), Expect = 1.800e-39
Identity = 82/96 (85.42%), Postives = 91/96 (94.79%), Query Frame = 1

		  

Query: 4   KIRYEALQSEAGKNLLRRSGRAPDDISSVVLVEKERSYIKSEAVLKIMEYINLPFPQMAF 63
           +IRYEALQSEAGK LLRRSGRAPDDISSVVLVEKERSYIKSEAVLKIMEYI+LPFPQ+AF
Sbjct: 83  RIRYEALQSEAGKKLLRRSGRAPDDISSVVLVEKERSYIKSEAVLKIMEYIDLPFPQLAF 142

Query: 64  FLKLLPLLVRDFAYDNLANNRYNIFGKSESCEIYLD 100
           FL+ +PL +RDFAYDN+ANNRY IFG+SESCE+Y D
Sbjct: 143 FLQFVPLFIRDFAYDNVANNRYTIFGRSESCEVYYD 178

BLAST of Spo03302.1 vs. NCBI nr
Match: gi|1000969557|ref|XP_015573792.1| (PREDICTED: DCC family protein At1g52590, chloroplastic [Ricinus communis])

HSP 1 Score: 168.3 bits (425), Expect = 6.800e-39
Identity = 82/96 (85.42%), Postives = 90/96 (93.75%), Query Frame = 1

		  

Query: 4   KIRYEALQSEAGKNLLRRSGRAPDDISSVVLVEKERSYIKSEAVLKIMEYINLPFPQMAF 63
           KIRYEALQSEAGK LLRRSGRAPDDISSVVLVEK+RSYIKSEAVLKIMEYI+LPFPQ+AF
Sbjct: 80  KIRYEALQSEAGKKLLRRSGRAPDDISSVVLVEKDRSYIKSEAVLKIMEYIDLPFPQLAF 139

Query: 64  FLKLLPLLVRDFAYDNLANNRYNIFGKSESCEIYLD 100
           FL+ +PL +RDF YDN+ANNRY IFG+SESCEIY D
Sbjct: 140 FLQFVPLFIRDFVYDNVANNRYTIFGRSESCEIYND 175

BLAST of Spo03302.1 vs. NCBI nr
Match: gi|460380539|ref|XP_004236013.1| (PREDICTED: DCC family protein At1g52590, chloroplastic isoform X2 [Solanum lycopersicum])

HSP 1 Score: 166.8 bits (421), Expect = 2.000e-38
Identity = 80/93 (86.02%), Postives = 90/93 (96.77%), Query Frame = 1

		  

Query: 4   KIRYEALQSEAGKNLLRRSGRAPDDISSVVLVEKERSYIKSEAVLKIMEYINLPFPQMAF 63
           +IRYEALQSEAGKNLLRRSGRAPDDISSVVLVEK+RSY+KSEAVLKIMEYINLPFPQ+AF
Sbjct: 91  RIRYEALQSEAGKNLLRRSGRAPDDISSVVLVEKDRSYVKSEAVLKIMEYINLPFPQLAF 150

Query: 64  FLKLLPLLVRDFAYDNLANNRYNIFGKSESCEI 97
           FL+ +PL +RDFAYDN+ANNRY +FG+SESCEI
Sbjct: 151 FLQFVPLFIRDFAYDNVANNRYALFGRSESCEI 183

BLAST of Spo03302.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RR17_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_044450 PE=4 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 4.000e-46
Identity = 97/97 (100.00%), Postives = 97/97 (100.00%), Query Frame = 1

		  

Query: 4   KIRYEALQSEAGKNLLRRSGRAPDDISSVVLVEKERSYIKSEAVLKIMEYINLPFPQMAF 63
           KIRYEALQSEAGKNLLRRSGRAPDDISSVVLVEKERSYIKSEAVLKIMEYINLPFPQMAF
Sbjct: 79  KIRYEALQSEAGKNLLRRSGRAPDDISSVVLVEKERSYIKSEAVLKIMEYINLPFPQMAF 138

Query: 64  FLKLLPLLVRDFAYDNLANNRYNIFGKSESCEIYLDQ 101
           FLKLLPLLVRDFAYDNLANNRYNIFGKSESCEIYLDQ
Sbjct: 139 FLKLLPLLVRDFAYDNLANNRYNIFGKSESCEIYLDQ 175

BLAST of Spo03302.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BH14_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_9g219690 PE=4 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 1.900e-40
Identity = 85/95 (89.47%), Postives = 91/95 (95.79%), Query Frame = 1

		  

Query: 5   IRYEALQSEAGKNLLRRSGRAPDDISSVVLVEKERSYIKSEAVLKIMEYINLPFPQMAFF 64
           IRYEALQSEAGK LLRRSGR+PDDISSVVLVEKERSYIKSEAVLKIMEYI+LPFPQ+AFF
Sbjct: 79  IRYEALQSEAGKKLLRRSGRSPDDISSVVLVEKERSYIKSEAVLKIMEYIDLPFPQLAFF 138

Query: 65  LKLLPLLVRDFAYDNLANNRYNIFGKSESCEIYLD 100
           L+ LPL +RDFAYDNLANNRY IFGKSESCEIY+D
Sbjct: 139 LQFLPLFIRDFAYDNLANNRYTIFGKSESCEIYVD 173

BLAST of Spo03302.1 vs. UniProtKB/TrEMBL
Match: E6NUB8_JATCU (JHL06P13.8 protein OS=Jatropha curcas GN=JHL06P13.8 PE=4 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 1.200e-39
Identity = 82/96 (85.42%), Postives = 91/96 (94.79%), Query Frame = 1

		  

Query: 4   KIRYEALQSEAGKNLLRRSGRAPDDISSVVLVEKERSYIKSEAVLKIMEYINLPFPQMAF 63
           +IRYEALQSEAGK LLRRSGRAPDDISSVVLVEKERSYIKSEAVLKIMEYI+LPFPQ+AF
Sbjct: 83  RIRYEALQSEAGKKLLRRSGRAPDDISSVVLVEKERSYIKSEAVLKIMEYIDLPFPQLAF 142

Query: 64  FLKLLPLLVRDFAYDNLANNRYNIFGKSESCEIYLD 100
           FL+ +PL +RDFAYDN+ANNRY IFG+SESCE+Y D
Sbjct: 143 FLQFVPLFIRDFAYDNVANNRYTIFGRSESCEVYYD 178

BLAST of Spo03302.1 vs. UniProtKB/TrEMBL
Match: A0A0V0HDK9_SOLCH (Putative DCC family protein, chloroplastic-like OS=Solanum chacoense PE=4 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 4.700e-39
Identity = 80/96 (83.33%), Postives = 92/96 (95.83%), Query Frame = 1

		  

Query: 4   KIRYEALQSEAGKNLLRRSGRAPDDISSVVLVEKERSYIKSEAVLKIMEYINLPFPQMAF 63
           +IRYEALQSEAGKNLLRRSGRAPDDISSVVLVEK+RSY+KSEAVLKIMEYINLPFPQ+AF
Sbjct: 91  RIRYEALQSEAGKNLLRRSGRAPDDISSVVLVEKDRSYVKSEAVLKIMEYINLPFPQLAF 150

Query: 64  FLKLLPLLVRDFAYDNLANNRYNIFGKSESCEIYLD 100
           FL+ +PL +RDFAYDN+ANNRY +FG+SESCEI ++
Sbjct: 151 FLQFVPLFIRDFAYDNVANNRYALFGRSESCEIXME 186

BLAST of Spo03302.1 vs. UniProtKB/TrEMBL
Match: K4BLF9_SOLLC (Uncharacterized protein OS=Solanum lycopersicum PE=4 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 1.400e-38
Identity = 80/93 (86.02%), Postives = 90/93 (96.77%), Query Frame = 1

		  

Query: 4   KIRYEALQSEAGKNLLRRSGRAPDDISSVVLVEKERSYIKSEAVLKIMEYINLPFPQMAF 63
           +IRYEALQSEAGKNLLRRSGRAPDDISSVVLVEK+RSY+KSEAVLKIMEYINLPFPQ+AF
Sbjct: 91  RIRYEALQSEAGKNLLRRSGRAPDDISSVVLVEKDRSYVKSEAVLKIMEYINLPFPQLAF 150

Query: 64  FLKLLPLLVRDFAYDNLANNRYNIFGKSESCEI 97
           FL+ +PL +RDFAYDN+ANNRY +FG+SESCEI
Sbjct: 151 FLQFVPLFIRDFAYDNVANNRYALFGRSESCEI 183

BLAST of Spo03302.1 vs. ExPASy Swiss-Prot
Match: Y1259_ARATH (DCC family protein At1g52590, chloroplastic OS=Arabidopsis thaliana GN=At1g52590 PE=2 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 3.500e-35
Identity = 72/92 (78.26%), Postives = 84/92 (91.30%), Query Frame = 1

		  

Query: 5   IRYEALQSEAGKNLLRRSGRAPDDISSVVLVEKERSYIKSEAVLKIMEYINLPFPQMAFF 64
           IR+EALQSEAGK LL RSGRAPDDISSVVLVE +RSYIKSEAVLKIM+YI+LPFPQ+AFF
Sbjct: 81  IRFEALQSEAGKKLLLRSGRAPDDISSVVLVENDRSYIKSEAVLKIMKYIDLPFPQLAFF 140

Query: 65  LKLLPLLVRDFAYDNLANNRYNIFGKSESCEI 97
           L+  PL VRDF Y+N+ANNRY +FG+S+SCE+
Sbjct: 141 LQFAPLFVRDFLYENVANNRYAMFGRSDSCEL 172

BLAST of Spo03302.1 vs. ExPASy Swiss-Prot
Match: YUXK_BACSU (Uncharacterized protein YuxK OS=Bacillus subtilis (strain 168) GN=yuxK PE=3 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 3.000e-10
Identity = 35/92 (38.04%), Postives = 50/92 (54.35%), Query Frame = 1

		  

Query: 3   GKIRYEALQSEAGKNLLRRSGRAPDDISSVVLVEKERSYIKSEAVLKIMEYINLPFPQMA 62
           G I + +LQSE G++LL++SG   D   S V +E  + Y KS A +K+  ++  P+    
Sbjct: 34  GLISFTSLQSETGQSLLKKSGLPTDRFDSFVFIEDGQVYTKSTAAIKVFRHLRGPWRLFV 93

Query: 63  FFLKLLPLLVRDFAYDNLANNRYNIFGKSESC 95
            F   +P  VRD  Y  +A NRY  FGK   C
Sbjct: 94  LFF-AVPKPVRDMVYSFIAKNRYKWFGKKNEC 124

BLAST of Spo03302.1 vs. TAIR (Arabidopsis)
Match: AT1G52590.1 (Putative thiol-disulphide oxidoreductase DCC)

HSP 1 Score: 148.7 bits (374), Expect = 2.000e-36
Identity = 72/92 (78.26%), Postives = 84/92 (91.30%), Query Frame = 1

		  

Query: 5   IRYEALQSEAGKNLLRRSGRAPDDISSVVLVEKERSYIKSEAVLKIMEYINLPFPQMAFF 64
           IR+EALQSEAGK LL RSGRAPDDISSVVLVE +RSYIKSEAVLKIM+YI+LPFPQ+AFF
Sbjct: 81  IRFEALQSEAGKKLLLRSGRAPDDISSVVLVENDRSYIKSEAVLKIMKYIDLPFPQLAFF 140

Query: 65  LKLLPLLVRDFAYDNLANNRYNIFGKSESCEI 97
           L+  PL VRDF Y+N+ANNRY +FG+S+SCE+
Sbjct: 141 LQFAPLFVRDFLYENVANNRYAMFGRSDSCEL 172

BLAST of Spo03302.1 vs. TAIR (Arabidopsis)
Match: AT1G24095.1 (Putative thiol-disulphide oxidoreductase DCC)

HSP 1 Score: 56.2 bits (134), Expect = 1.300e-8
Identity = 33/99 (33.33%), Postives = 55/99 (55.56%), Query Frame = 1

		  

Query: 4   KIRYEALQSEAGKNLLRRSGRAPDDISSVVLVEKERSYIK--SEAVLKIMEYINLPFPQM 63
           KI++  LQS+A +  L  SG   +D+    L  +   +    S A L+++ Y+ LP+  +
Sbjct: 100 KIKFCCLQSKAAEPYLEVSGVTREDVQKRFLFIEGLGFYHQASTAALRVVSYLPLPYSAL 159

Query: 64  AFFLKLLPLLVRDFAYDNLANNRYNIFGKSESCEIYLDQ 101
             F  ++P  +RD  YD +A NRY+ FGK+E C +  D+
Sbjct: 160 NAF-SIVPTPLRDSVYDYVAKNRYDWFGKAEDCLVLNDK 197

The following BLAST results are available for this feature:
BLAST of Spo03302.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902228833|gb|KNA21277.1|5.7e-46100.hypothetical protein SOVF_0444... [more]
gi|731359191|ref|XP_010691152.1|2.7e-4089.4PREDICTED: DCC family protein ... [more]
gi|802603672|ref|XP_012073206.1|1.8e-3985.4PREDICTED: DCC family protein ... [more]
gi|1000969557|ref|XP_015573792.1|6.8e-3985.4PREDICTED: DCC family protein ... [more]
gi|460380539|ref|XP_004236013.1|2.0e-3886.0PREDICTED: DCC family protein ... [more]
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BLAST of Spo03302.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9RR17_SPIOL4.0e-46100.Uncharacterized protein OS=Spi... [more]
A0A0J8BH14_BETVU1.9e-4089.4Uncharacterized protein OS=Bet... [more]
E6NUB8_JATCU1.2e-3985.4JHL06P13.8 protein OS=Jatropha... [more]
A0A0V0HDK9_SOLCH4.7e-3983.3Putative DCC family protein, c... [more]
K4BLF9_SOLLC1.4e-3886.0Uncharacterized protein OS=Sol... [more]
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BLAST of Spo03302.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 2
Match NameE-valueIdentityDescription
Y1259_ARATH3.5e-3578.2DCC family protein At1g52590, ... [more]
YUXK_BACSU3.0e-1038.0Uncharacterized protein YuxK O... [more]
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BLAST of Spo03302.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 2
Match NameE-valueIdentityDescription
AT1G52590.12.0e-3678.2Putative thiol-disulphide oxid... [more]
AT1G24095.11.3e-833.3Putative thiol-disulphide oxid... [more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007263Protein of unknown function DUF393PFAMPF04134DUF393coord: 3..90
score: 5.4
NoneNo IPR availablePANTHERPTHR33639FAMILY NOT NAMEDcoord: 3..100
score: 4.2
NoneNo IPR availablePANTHERPTHR33639:SF2SUBFAMILY NOT NAMEDcoord: 3..100
score: 4.2

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019288 isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
biological_process GO:0006364 rRNA processing
biological_process GO:0009231 riboflavin biosynthetic process
biological_process GO:0010027 thylakoid membrane organization
biological_process GO:0015995 chlorophyll biosynthetic process
biological_process GO:0009902 chloroplast relocation
biological_process GO:0042742 defense response to bacterium
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0006098 pentose-phosphate shunt
biological_process GO:0009773 photosynthetic electron transport in photosystem I
biological_process GO:0042793 plastid transcription
cellular_component GO:0005840 ribosome
cellular_component GO:0005634 nucleus
cellular_component GO:0009570 chloroplast stroma
cellular_component GO:0010287 plastoglobule
cellular_component GO:0009349 riboflavin synthase complex
cellular_component GO:0009507 chloroplast
molecular_function GO:0004746 riboflavin synthase activity
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0003723 RNA binding
RNA-Seq Expression