Spo04116 (gene)

Overview
NameSpo04116
Typegene
OrganismSpinacia oleracea (Spinach)
DescriptionCysteine proteinase inhibitor
LocationSpoScf_00647 : 259276 .. 263520 (-)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAGAATACAAGAAATCGTTCGTCTCGCAAATCCATCTCTTCTTCTCTTTTAAAACTTCTACATTAAGCAAAAACGAAGGCAAAATCTTCTATAAATCTATGTTGAGCATCTTCTTCCTCTGTCACCACTCTTCAACTCTCATTCCCTATCTCATTTTATCTATCCTCATCTTCTGTCATCAAGGATCAACAGGGTCTTGCATCGACTTTGAATCTGAACCGTCAATGGCTACTCTCGGTGGTTTGCGCGAATCCCAAGGTGCTTCCAATGATGCTGAGATTGAAAGCCTTGCCCGTTTTGCTGTCGACGAACACAACAAGAAAGAGGTTTACTAATTTTTTAAATTAAACGCTTTGTTTTTTTTTGCAGAATTTGAAGTTGCGCATTTCATAGTTTCGCATGTTTTACTGGTAATTTGTGGGTTTATTTTTTATTTTTTGATGTCCTGATTTTCTGCAAATTTTGATGGTACCCGGTTCATAGTTCTGTGTGATATTTTCTGGGATTTGGATTTTTCTGTTAACAGAATTTGAAGTTGCCCATTTCACAATTTTACAGGATTTACTGCTATATTTTGTTTTTTCTTGCCGATTCCTTTTTTTTTGAAGTTGGGGTTTGATCAGAAGATGCTTTGTTTTTCCCCTTTTTCTCACCCAATTACGATTTCGGTATCTTGATTTTTATGTTTTTTTTTCAATTGCTATGTTTAATTTTGGGATTTGACCTTCTTAGGTCTTGCATAATATTTGTGTCAAAAGTTGTTGCCCATTTTGGTTTTATTCTATAGTGTGAATAAGAGGAGTCGTCCCGGGTTTCATTGTTAGACTCATCTTAATTTGTGATATCAAGACATTACTAAAGATAATTAAAGATATTAGTGTTGAAAGTTATGTCTCCGACCGTAAACAAGGCAATTGGGTAAGTCTTTGGGAGGATGAAGAGTAATAAGTTTAATTTTTGTTGGTAGTTTTGGGTTTGTGAAAGGATTCACTTGACAATTGTTGTGCATATTACTCTTCTCACTTTTCTCTTTGTTTGAATGAAGAATGCCTTGCTGGAGTTTGCAAGGATTATAAAGGCAAAGGAACAGGTAGTTGCGGGTACGTTACATCACTTCACAATCGAAGCGATCGATTCTGGCAAGAAGAAGCTGTATGAAGCAAAAGTATGGGTGAAGCCATGGATGAACTTCAAGGAGCTTCAGGAATTCAAGCATACTGAGGAAAGCCATGCAATCACTTCTTCTGACCTTGGTGCTAATAAAGGTAATGATTAAGGTTCATAATTTTCGCCGAAAACTATTAGTGTTGTTGGATATAATAAGAAGAATCTCAACATATATGAATAGTAGTAGCTTGATCTTCAAGGTTGTAAATTCCCGTTTTATCATGACCAAGGGTTCCTTTAAGAAGAATGTTCGGAGGAACACTAGCAGCTTAACCTATGCTTTATTCATACACGATAGGCCTTATTTGAAATTATTGAATGAATAGCTCAAGACATCTTTCTAAAGGACTACATAGTGTGTATAGGCTCAAAGCTACTTGACTTGGATATTGTTGCTGTGATACTGTGAGTGGTTGTTTGCCACCCATCCATAATGACTACCCTGCTTTCTATGTGTTGCTATCTGAATTTGATTGTTTAGAAAGGTATCTTCTGGTGAGTGTATTGTCTACTCCTAGTCATATTTTTTTATAGAGACGCAGCTAACAGTTCGTGTTAAGTTGAGAGAGAGTGTGAATGTGTTGCTTTAATAGACGAATTCCACTTCCGTTTTATCCTTGTTTCTTCTTCTCGAGCGTGTAGCTCCCTTGAAGTGCAATAAATGCAAGACATTTCTCTTTAATGATAAATTTCAGGGTTGGATGTAATTTGGATTGAGATCAGGAATACCTCATGACATCTTTAACAAAATGCCTAATAGAAATGTGTGTCTTTTGTTATTGTCATGTTCAATGGAAATTGCCTGTCACAGATGATCCAGTATCATCCTTAATTCAGCTTTTGATTGTTCCGTTCTGGTTCTATGAAAAGAATACGACTGAAAAATATATGCTTCATTTTGTATTCTACCGTTCTACGTGGAGATTCGAGTTTTTTGGTTCTGAGCATGGAGACCAATCAATCAATTCTTCTGCAGAAAGAGAATTGCATTCTTTATCTGTTTATTGAAGCCACTAATAAACTGATTAATTTTGGAGTTCCATGAAGCATCTTTCATACATTATTTTTCCAATATGATCTAATGGTGTAATAAGTTGTGTTGTGTGGTCAATGAATCATCTTTCTTGTTTTTGTACTTCTAAGTGATTGTTAGCATGCTGGCACTAACCTATGGTTAAATGATCAATCAGAAGGACATGGCGCAGGATGGAAAGAAGTGCCAGTCCATGACCCTGAAGTGCAAAATGCAGCAAATCATGCTCTTCAGACCATACAGCAAAGATCCAACTCCTTATTCCCTTATGAACTGCAAGAAGTGTCTCATGCGAAGGCTGAGGTTCGTCCTTCTGTTACCTACTGACACTTTTTAGTTACTCTTTCTTTTCCTCCTCTACTTAGAGAAAGCTAGATGAAAAATATTCCATCCTTCATCAAGTAAATAGGTCACAGATGATTTTGAGCTAATTTTTCAAGAAACTTAGTGAAAATACCACACCTTCTGACTTTTAACGGCTTATCATATTCGACATTGACCTTTTTAAGGGTAATTTCAAAACCGCATTTACTAAATTTAGAATGTGACCTACTATTTGGGATTATGTGAGTAAAATTCTTGCTTTATTATTTTGGAACAGAGATAGTATATTCTTTTAATATCTTACACTTTATGAATTCGGAATAGCACAATACCAAAGATTTTGGTAATCCTTGAGTATTTTTATGTGAGTTGCACTTTCTAAAGTTGTCCGATTTAATATGAGTTACACTTCCTATTTTTTGGTATGTCATGGTATATTGGTATCTACTCCCAATTATATTTATCTTCCTCNTCAAAACCGCATTTACTAAATTTAGAATGTGACCTACTATTTGGGATTATGTGAGTAAAATTCTTGCTTTATTATTTTGGAACAGAGATAGTATATTCTTTTAATATCTTACACTTTATGAATTCGGAATAGCACAATACCAAAGATTTTGGTAATCCTTGAGTATTTTTATGTGAGTTACACTTTCTAAAGTTGTCCGATTTAATATGAGTTACACTTCCTATTTTTTGGTATGTCATGGTATATTGGTATCTACTCCCAATTATATTTATCTTCCTCTTCCTCTTCCTCATGGTTCAGCACTTTACATTTTCAATATGAGTTGCATTTTCTATCTTTGATATGTTGTGGTATCCACTCCCAATTAAATAATCTTATTTTTCACTCTTATCGTCTATGCACTTAATTTACATCCTCTTTTTTCCACAATAAATAATTAACTCACTGATAATAAATAATAACATACTACCCCACTTGCTGTTACTTAAATAATATCAACTCATTTTTTTAACATTCCGTATCTGTCAAACTGTGTAACTCATATTAAAATACGGAAAGAGTAATTAGATGTGAGGATTGGGATCAATTTTCATTAGTATATCCTGCCTCACTAATCCCTACTAGTTATTGACCCGAAGGAATTTGTTGATATAGGTGTGAGAATATACATATCTTGCACCCACTAGTTTTCATAGTTTTTCAAGTTTTGTGTAATTGTGGGCCTTGTGGCAACATGTGTTTGAAGTCTGATGTGAAAGTGGTTATATGACAGGTTGTGGATGACACTGCTAAGTTTAACCTGCACCTCAAAGTGAAGAGAGGAAACAAGGAGGAGAAATTTAATGTGGAGGTGCACAAAAACAGTCAAGGAAACTATAACCTTAATCAAATGGGAAACATCGAGCCTGAGTTCGAGAACAAGGAATGAGTTTTGGACTTCTTTGTTTGTTAATTAACTGTGACTGTGAGCACTTAGAATAGTATGTAATGAATGCAAGCTGACTTGCTCTGAGTATAATAAGCCTTGTCTTTTGTTACGGTTACACCGTCGTTTCTTCTATATGGATATTATAGAATTTATAGCTATGGATGTATATGAAATGGAGATTGCTATCTTGCTTTTAACAACTTCCCTACTCTGTTGAAATGCTGCCTTAACATGTTTATATTAGTTAAAGTGTTCTCTTGATTTTTGTTTAGGAAGATTTTAATTCTTCCGGCTTTGATTTCTTTGACTAAAAATCG

mRNA sequence

ATGAAAGAATACAAGAAATCGTTCGTCTCGCAAATCCATCTCTTCTTCTCTTTTAAAACTTCTACATTAAGCAAAAACGAAGGCAAAATCTTCTATAAATCTATGTTGAGCATCTTCTTCCTCTGTCACCACTCTTCAACTCTCATTCCCTATCTCATTTTATCTATCCTCATCTTCTGTCATCAAGGATCAACAGGGTCTTGCATCGACTTTGAATCTGAACCGTCAATGGCTACTCTCGGTGGTTTGCGCGAATCCCAAGGTGCTTCCAATGATGCTGAGATTGAAAGCCTTGCCCGTTTTGCTGTCGACGAACACAACAAGAAAGAGAATGCCTTGCTGGAGTTTGCAAGGATTATAAAGGCAAAGGAACAGGTAGTTGCGGGTACGTTACATCACTTCACAATCGAAGCGATCGATTCTGGCAAGAAGAAGCTGTATGAAGCAAAAGTATGGGTGAAGCCATGGATGAACTTCAAGGAGCTTCAGGAATTCAAGCATACTGAGGAAAGCCATGCAATCACTTCTTCTGACCTTGGTGCTAATAAAGAAGGACATGGCGCAGGATGGAAAGAAGTGCCAGTCCATGACCCTGAAGTGCAAAATGCAGCAAATCATGCTCTTCAGACCATACAGCAAAGATCCAACTCCTTATTCCCTTATGAACTGCAAGAAGTGTCTCATGCGAAGGCTGAGGTTGTGGATGACACTGCTAAGTTTAACCTGCACCTCAAAGTGAAGAGAGGAAACAAGGAGGAGAAATTTAATGTGGAGGTGCACAAAAACAGTCAAGGAAACTATAACCTTAATCAAATGGGAAACATCGAGCCTGAGTTCGAGAACAAGGAATGAGTTTTGGACTTCTTTGTTTGTTAATTAACTGTGACTGTGAGCACTTAGAATAGTATGTAATGAATGCAAGCTGACTTGCTCTGAGTATAATAAGCCTTGTCTTTTGTTACGGTTACACCGTCGTTTCTTCTATATGGATATTATAGAATTTATAGCTATGGATGTATATGAAATGGAGATTGCTATCTTGCTTTTAACAACTTCCCTACTCTGTTGAAATGCTGCCTTAACATGTTTATATTAGTTAAAGTGTTCTCTTGATTTTTGTTTAGGAAGATTTTAATTCTTCCGGCTTTGATTTCTTTGACTAAAAATCG

Coding sequence (CDS)

ATGAAAGAATACAAGAAATCGTTCGTCTCGCAAATCCATCTCTTCTTCTCTTTTAAAACTTCTACATTAAGCAAAAACGAAGGCAAAATCTTCTATAAATCTATGTTGAGCATCTTCTTCCTCTGTCACCACTCTTCAACTCTCATTCCCTATCTCATTTTATCTATCCTCATCTTCTGTCATCAAGGATCAACAGGGTCTTGCATCGACTTTGAATCTGAACCGTCAATGGCTACTCTCGGTGGTTTGCGCGAATCCCAAGGTGCTTCCAATGATGCTGAGATTGAAAGCCTTGCCCGTTTTGCTGTCGACGAACACAACAAGAAAGAGAATGCCTTGCTGGAGTTTGCAAGGATTATAAAGGCAAAGGAACAGGTAGTTGCGGGTACGTTACATCACTTCACAATCGAAGCGATCGATTCTGGCAAGAAGAAGCTGTATGAAGCAAAAGTATGGGTGAAGCCATGGATGAACTTCAAGGAGCTTCAGGAATTCAAGCATACTGAGGAAAGCCATGCAATCACTTCTTCTGACCTTGGTGCTAATAAAGAAGGACATGGCGCAGGATGGAAAGAAGTGCCAGTCCATGACCCTGAAGTGCAAAATGCAGCAAATCATGCTCTTCAGACCATACAGCAAAGATCCAACTCCTTATTCCCTTATGAACTGCAAGAAGTGTCTCATGCGAAGGCTGAGGTTGTGGATGACACTGCTAAGTTTAACCTGCACCTCAAAGTGAAGAGAGGAAACAAGGAGGAGAAATTTAATGTGGAGGTGCACAAAAACAGTCAAGGAAACTATAACCTTAATCAAATGGGAAACATCGAGCCTGAGTTCGAGAACAAGGAATGA

Protein sequence

MKEYKKSFVSQIHLFFSFKTSTLSKNEGKIFYKSMLSIFFLCHHSSTLIPYLILSILIFCHQGSTGSCIDFESEPSMATLGGLRESQGASNDAEIESLARFAVDEHNKKENALLEFARIIKAKEQVVAGTLHHFTIEAIDSGKKKLYEAKVWVKPWMNFKELQEFKHTEESHAITSSDLGANKEGHGAGWKEVPVHDPEVQNAANHALQTIQQRSNSLFPYELQEVSHAKAEVVDDTAKFNLHLKVKRGNKEEKFNVEVHKNSQGNYNLNQMGNIEPEFENKE
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo04116.1Spo04116.1mRNA


Homology
[Close]
BLAST of Spo04116.1 vs. NCBI nr
Match: gi|902212731|gb|KNA17069.1| (hypothetical protein SOVF_083420 isoform A [Spinacia oleracea])

HSP 1 Score: 558.5 bits (1438), Expect = 6.600e-156
Identity = 283/283 (100.00%), Postives = 283/283 (100.00%), Query Frame = 1 

		  

Query: 1   MKEYKKSFVSQIHLFFSFKTSTLSKNEGKIFYKSMLSIFFLCHHSSTLIPYLILSILIFC 60
           MKEYKKSFVSQIHLFFSFKTSTLSKNEGKIFYKSMLSIFFLCHHSSTLIPYLILSILIFC
Sbjct: 1   MKEYKKSFVSQIHLFFSFKTSTLSKNEGKIFYKSMLSIFFLCHHSSTLIPYLILSILIFC 60

Query: 61  HQGSTGSCIDFESEPSMATLGGLRESQGASNDAEIESLARFAVDEHNKKENALLEFARII 120
           HQGSTGSCIDFESEPSMATLGGLRESQGASNDAEIESLARFAVDEHNKKENALLEFARII
Sbjct: 61  HQGSTGSCIDFESEPSMATLGGLRESQGASNDAEIESLARFAVDEHNKKENALLEFARII 120

Query: 121 KAKEQVVAGTLHHFTIEAIDSGKKKLYEAKVWVKPWMNFKELQEFKHTEESHAITSSDLG 180
           KAKEQVVAGTLHHFTIEAIDSGKKKLYEAKVWVKPWMNFKELQEFKHTEESHAITSSDLG
Sbjct: 121 KAKEQVVAGTLHHFTIEAIDSGKKKLYEAKVWVKPWMNFKELQEFKHTEESHAITSSDLG 180

Query: 181 ANKEGHGAGWKEVPVHDPEVQNAANHALQTIQQRSNSLFPYELQEVSHAKAEVVDDTAKF 240
           ANKEGHGAGWKEVPVHDPEVQNAANHALQTIQQRSNSLFPYELQEVSHAKAEVVDDTAKF
Sbjct: 181 ANKEGHGAGWKEVPVHDPEVQNAANHALQTIQQRSNSLFPYELQEVSHAKAEVVDDTAKF 240

Query: 241 NLHLKVKRGNKEEKFNVEVHKNSQGNYNLNQMGNIEPEFENKE 284
           NLHLKVKRGNKEEKFNVEVHKNSQGNYNLNQMGNIEPEFENKE
Sbjct: 241 NLHLKVKRGNKEEKFNVEVHKNSQGNYNLNQMGNIEPEFENKE 283
[Close]
BLAST of Spo04116.1 vs. NCBI nr
Match: gi|902212732|gb|KNA17070.1| (hypothetical protein SOVF_083420 isoform B [Spinacia oleracea])

HSP 1 Score: 552.4 bits (1422), Expect = 4.700e-154
Identity = 282/283 (99.65%), Postives = 282/283 (99.65%), Query Frame = 1 

		  

Query: 1   MKEYKKSFVSQIHLFFSFKTSTLSKNEGKIFYKSMLSIFFLCHHSSTLIPYLILSILIFC 60
           MKEYKKSFVSQIHLFFSFKTSTLSKNEGKIFYKSMLSIFFLCHHSSTLIPYLILSILIFC
Sbjct: 1   MKEYKKSFVSQIHLFFSFKTSTLSKNEGKIFYKSMLSIFFLCHHSSTLIPYLILSILIFC 60

Query: 61  HQGSTGSCIDFESEPSMATLGGLRESQGASNDAEIESLARFAVDEHNKKENALLEFARII 120
           HQGSTGSCIDFESEPSMATLGGLRESQGASNDAEIESLARFAVDEHNKKENALLEFARII
Sbjct: 61  HQGSTGSCIDFESEPSMATLGGLRESQGASNDAEIESLARFAVDEHNKKENALLEFARII 120

Query: 121 KAKEQVVAGTLHHFTIEAIDSGKKKLYEAKVWVKPWMNFKELQEFKHTEESHAITSSDLG 180
           KAKEQVVAGTLHHFTIEAIDSGKKKLYEAKVWVKPWMNFKELQEFKHTEESHAITSSDLG
Sbjct: 121 KAKEQVVAGTLHHFTIEAIDSGKKKLYEAKVWVKPWMNFKELQEFKHTEESHAITSSDLG 180

Query: 181 ANKEGHGAGWKEVPVHDPEVQNAANHALQTIQQRSNSLFPYELQEVSHAKAEVVDDTAKF 240
           ANK GHGAGWKEVPVHDPEVQNAANHALQTIQQRSNSLFPYELQEVSHAKAEVVDDTAKF
Sbjct: 181 ANK-GHGAGWKEVPVHDPEVQNAANHALQTIQQRSNSLFPYELQEVSHAKAEVVDDTAKF 240

Query: 241 NLHLKVKRGNKEEKFNVEVHKNSQGNYNLNQMGNIEPEFENKE 284
           NLHLKVKRGNKEEKFNVEVHKNSQGNYNLNQMGNIEPEFENKE
Sbjct: 241 NLHLKVKRGNKEEKFNVEVHKNSQGNYNLNQMGNIEPEFENKE 282
[Close]
BLAST of Spo04116.1 vs. NCBI nr
Match: gi|110748606|gb|ABG89856.1| (cystatin [Amaranthus hypochondriacus])

HSP 1 Score: 398.7 bits (1023), Expect = 8.700e-108
Identity = 199/248 (80.24%), Postives = 224/248 (90.32%), Query Frame = 1 

		  

Query: 35  MLSIFFLCHHSSTLIPYLILSILIFCHQGSTGSCIDFESEPSMATLGGLRESQGASNDAE 94
           ++   FL  HSST++  L+ S++ F    S GSC DFESEPSMATLGGLRESQGA+NDAE
Sbjct: 2   LIKFSFLLPHSSTIL--LLFSLIFFFSPSSQGSCSDFESEPSMATLGGLRESQGAANDAE 61

Query: 95  IESLARFAVDEHNKKENALLEFARIIKAKEQVVAGTLHHFTIEAIDSGKKKLYEAKVWVK 154
           IESLARFAVDEHNKKENALLEFAR++KAKEQVVAGTLHHFTIEAID+GKKKLY+AKVWVK
Sbjct: 62  IESLARFAVDEHNKKENALLEFARVVKAKEQVVAGTLHHFTIEAIDAGKKKLYDAKVWVK 121

Query: 155 PWMNFKELQEFKHTEESHAITSSDLGANKEGHGAGWKEVPVHDPEVQNAANHALQTIQQR 214
           PWMNFKELQEFKHTE+S + TSSDLGA +EGH  GWKEVPVHDPEVQNAA HA++TIQQR
Sbjct: 122 PWMNFKELQEFKHTEDSPSFTSSDLGAIREGHAPGWKEVPVHDPEVQNAAEHAVKTIQQR 181

Query: 215 SNSLFPYELQEVSHAKAEVVDDTAKFNLHLKVKRGNKEEKFNVEVHKNSQGNYNLNQMGN 274
           SNSLFPYELQE++HAKAEVV+DTAKFNLHLKVKRGNK+E FNVEVHK+S GNYNLN+MGN
Sbjct: 182 SNSLFPYELQEIAHAKAEVVEDTAKFNLHLKVKRGNKDEIFNVEVHKSSDGNYNLNKMGN 241

Query: 275 IEPEFENK 283
           I+PE EN+
Sbjct: 242 IQPEIENQ 247
[Close]
BLAST of Spo04116.1 vs. NCBI nr
Match: gi|731320021|ref|XP_010670580.1| (PREDICTED: cysteine proteinase inhibitor 6 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 383.3 bits (983), Expect = 3.800e-103
Identity = 190/222 (85.59%), Postives = 209/222 (94.14%), Query Frame = 1 

		  

Query: 61  HQGSTGSCIDFESEP--SMATLGGLRESQGASNDAEIESLARFAVDEHNKKENALLEFAR 120
           +QGS GSC DFES+   SMATLGGLRESQGASNDAEIESLARFAV+EHNKKENALLEFAR
Sbjct: 28  NQGSKGSCSDFESQEQSSMATLGGLRESQGASNDAEIESLARFAVEEHNKKENALLEFAR 87

Query: 121 IIKAKEQVVAGTLHHFTIEAIDSGKKKLYEAKVWVKPWMNFKELQEFKHTEESHAITSSD 180
           ++KAKEQVVAGTLHHFTIEAI++GKKKLY+AKVWVKPWMNFKELQEFKH ++S +IT SD
Sbjct: 88  VVKAKEQVVAGTLHHFTIEAIEAGKKKLYDAKVWVKPWMNFKELQEFKHADDSPSITPSD 147

Query: 181 LGANKEGHGAGWKEVPVHDPEVQNAANHALQTIQQRSNSLFPYELQEVSHAKAEVVDDTA 240
           LGA KEGH  GWKEVPVHDPEVQ+AANHA++TIQQRSNSLFPYELQE++HAKAEVV+DTA
Sbjct: 148 LGAIKEGHAPGWKEVPVHDPEVQDAANHAVKTIQQRSNSLFPYELQEIAHAKAEVVEDTA 207

Query: 241 KFNLHLKVKRGNKEEKFNVEVHKNSQGNYNLNQMGNIEPEFE 281
           KFNLHLKVKRGNKEE FNVEVHKN++GNYNLNQM NIEPEFE
Sbjct: 208 KFNLHLKVKRGNKEEIFNVEVHKNNEGNYNLNQMNNIEPEFE 249
[Close]
BLAST of Spo04116.1 vs. NCBI nr
Match: gi|870866005|gb|KMT17034.1| (hypothetical protein BVRB_2g042350 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 365.5 bits (937), Expect = 8.200e-98
Identity = 179/204 (87.75%), Postives = 196/204 (96.08%), Query Frame = 1 

		  

Query: 77  MATLGGLRESQGASNDAEIESLARFAVDEHNKKENALLEFARIIKAKEQVVAGTLHHFTI 136
           MATLGGLRESQGASNDAEIESLARFAV+EHNKKENALLEFAR++KAKEQVVAGTLHHFTI
Sbjct: 1   MATLGGLRESQGASNDAEIESLARFAVEEHNKKENALLEFARVVKAKEQVVAGTLHHFTI 60

Query: 137 EAIDSGKKKLYEAKVWVKPWMNFKELQEFKHTEESHAITSSDLGANKEGHGAGWKEVPVH 196
           EAI++GKKKLY+AKVWVKPWMNFKELQEFKH ++S +IT SDLGA KEGH  GWKEVPVH
Sbjct: 61  EAIEAGKKKLYDAKVWVKPWMNFKELQEFKHADDSPSITPSDLGAIKEGHAPGWKEVPVH 120

Query: 197 DPEVQNAANHALQTIQQRSNSLFPYELQEVSHAKAEVVDDTAKFNLHLKVKRGNKEEKFN 256
           DPEVQ+AANHA++TIQQRSNSLFPYELQE++HAKAEVV+DTAKFNLHLKVKRGNKEE FN
Sbjct: 121 DPEVQDAANHAVKTIQQRSNSLFPYELQEIAHAKAEVVEDTAKFNLHLKVKRGNKEEIFN 180

Query: 257 VEVHKNSQGNYNLNQMGNIEPEFE 281
           VEVHKN++GNYNLNQM NIEPEFE
Sbjct: 181 VEVHKNNEGNYNLNQMNNIEPEFE 204
[Close]
BLAST of Spo04116.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RDK0_SPIOL (Cysteine proteinase inhibitor OS=Spinacia oleracea GN=SOVF_083420 PE=3 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 4.600e-156
Identity = 283/283 (100.00%), Postives = 283/283 (100.00%), Query Frame = 1 

		  

Query: 1   MKEYKKSFVSQIHLFFSFKTSTLSKNEGKIFYKSMLSIFFLCHHSSTLIPYLILSILIFC 60
           MKEYKKSFVSQIHLFFSFKTSTLSKNEGKIFYKSMLSIFFLCHHSSTLIPYLILSILIFC
Sbjct: 1   MKEYKKSFVSQIHLFFSFKTSTLSKNEGKIFYKSMLSIFFLCHHSSTLIPYLILSILIFC 60

Query: 61  HQGSTGSCIDFESEPSMATLGGLRESQGASNDAEIESLARFAVDEHNKKENALLEFARII 120
           HQGSTGSCIDFESEPSMATLGGLRESQGASNDAEIESLARFAVDEHNKKENALLEFARII
Sbjct: 61  HQGSTGSCIDFESEPSMATLGGLRESQGASNDAEIESLARFAVDEHNKKENALLEFARII 120

Query: 121 KAKEQVVAGTLHHFTIEAIDSGKKKLYEAKVWVKPWMNFKELQEFKHTEESHAITSSDLG 180
           KAKEQVVAGTLHHFTIEAIDSGKKKLYEAKVWVKPWMNFKELQEFKHTEESHAITSSDLG
Sbjct: 121 KAKEQVVAGTLHHFTIEAIDSGKKKLYEAKVWVKPWMNFKELQEFKHTEESHAITSSDLG 180

Query: 181 ANKEGHGAGWKEVPVHDPEVQNAANHALQTIQQRSNSLFPYELQEVSHAKAEVVDDTAKF 240
           ANKEGHGAGWKEVPVHDPEVQNAANHALQTIQQRSNSLFPYELQEVSHAKAEVVDDTAKF
Sbjct: 181 ANKEGHGAGWKEVPVHDPEVQNAANHALQTIQQRSNSLFPYELQEVSHAKAEVVDDTAKF 240

Query: 241 NLHLKVKRGNKEEKFNVEVHKNSQGNYNLNQMGNIEPEFENKE 284
           NLHLKVKRGNKEEKFNVEVHKNSQGNYNLNQMGNIEPEFENKE
Sbjct: 241 NLHLKVKRGNKEEKFNVEVHKNSQGNYNLNQMGNIEPEFENKE 283
[Close]
BLAST of Spo04116.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RC70_SPIOL (Cysteine proteinase inhibitor OS=Spinacia oleracea GN=SOVF_083420 PE=3 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 3.300e-154
Identity = 282/283 (99.65%), Postives = 282/283 (99.65%), Query Frame = 1 

		  

Query: 1   MKEYKKSFVSQIHLFFSFKTSTLSKNEGKIFYKSMLSIFFLCHHSSTLIPYLILSILIFC 60
           MKEYKKSFVSQIHLFFSFKTSTLSKNEGKIFYKSMLSIFFLCHHSSTLIPYLILSILIFC
Sbjct: 1   MKEYKKSFVSQIHLFFSFKTSTLSKNEGKIFYKSMLSIFFLCHHSSTLIPYLILSILIFC 60

Query: 61  HQGSTGSCIDFESEPSMATLGGLRESQGASNDAEIESLARFAVDEHNKKENALLEFARII 120
           HQGSTGSCIDFESEPSMATLGGLRESQGASNDAEIESLARFAVDEHNKKENALLEFARII
Sbjct: 61  HQGSTGSCIDFESEPSMATLGGLRESQGASNDAEIESLARFAVDEHNKKENALLEFARII 120

Query: 121 KAKEQVVAGTLHHFTIEAIDSGKKKLYEAKVWVKPWMNFKELQEFKHTEESHAITSSDLG 180
           KAKEQVVAGTLHHFTIEAIDSGKKKLYEAKVWVKPWMNFKELQEFKHTEESHAITSSDLG
Sbjct: 121 KAKEQVVAGTLHHFTIEAIDSGKKKLYEAKVWVKPWMNFKELQEFKHTEESHAITSSDLG 180

Query: 181 ANKEGHGAGWKEVPVHDPEVQNAANHALQTIQQRSNSLFPYELQEVSHAKAEVVDDTAKF 240
           ANK GHGAGWKEVPVHDPEVQNAANHALQTIQQRSNSLFPYELQEVSHAKAEVVDDTAKF
Sbjct: 181 ANK-GHGAGWKEVPVHDPEVQNAANHALQTIQQRSNSLFPYELQEVSHAKAEVVDDTAKF 240

Query: 241 NLHLKVKRGNKEEKFNVEVHKNSQGNYNLNQMGNIEPEFENKE 284
           NLHLKVKRGNKEEKFNVEVHKNSQGNYNLNQMGNIEPEFENKE
Sbjct: 241 NLHLKVKRGNKEEKFNVEVHKNSQGNYNLNQMGNIEPEFENKE 282
[Close]
BLAST of Spo04116.1 vs. UniProtKB/TrEMBL
Match: Q0GPA4_AMAHP (Cysteine proteinase inhibitor OS=Amaranthus hypochondriacus GN=CPI PE=2 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 6.100e-108
Identity = 199/248 (80.24%), Postives = 224/248 (90.32%), Query Frame = 1 

		  

Query: 35  MLSIFFLCHHSSTLIPYLILSILIFCHQGSTGSCIDFESEPSMATLGGLRESQGASNDAE 94
           ++   FL  HSST++  L+ S++ F    S GSC DFESEPSMATLGGLRESQGA+NDAE
Sbjct: 2   LIKFSFLLPHSSTIL--LLFSLIFFFSPSSQGSCSDFESEPSMATLGGLRESQGAANDAE 61

Query: 95  IESLARFAVDEHNKKENALLEFARIIKAKEQVVAGTLHHFTIEAIDSGKKKLYEAKVWVK 154
           IESLARFAVDEHNKKENALLEFAR++KAKEQVVAGTLHHFTIEAID+GKKKLY+AKVWVK
Sbjct: 62  IESLARFAVDEHNKKENALLEFARVVKAKEQVVAGTLHHFTIEAIDAGKKKLYDAKVWVK 121

Query: 155 PWMNFKELQEFKHTEESHAITSSDLGANKEGHGAGWKEVPVHDPEVQNAANHALQTIQQR 214
           PWMNFKELQEFKHTE+S + TSSDLGA +EGH  GWKEVPVHDPEVQNAA HA++TIQQR
Sbjct: 122 PWMNFKELQEFKHTEDSPSFTSSDLGAIREGHAPGWKEVPVHDPEVQNAAEHAVKTIQQR 181

Query: 215 SNSLFPYELQEVSHAKAEVVDDTAKFNLHLKVKRGNKEEKFNVEVHKNSQGNYNLNQMGN 274
           SNSLFPYELQE++HAKAEVV+DTAKFNLHLKVKRGNK+E FNVEVHK+S GNYNLN+MGN
Sbjct: 182 SNSLFPYELQEIAHAKAEVVEDTAKFNLHLKVKRGNKDEIFNVEVHKSSDGNYNLNKMGN 241

Query: 275 IEPEFENK 283
           I+PE EN+
Sbjct: 242 IQPEIENQ 247
[Close]
BLAST of Spo04116.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CY51_BETVU (Cysteine proteinase inhibitor OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g042350 PE=3 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 5.700e-98
Identity = 179/204 (87.75%), Postives = 196/204 (96.08%), Query Frame = 1 

		  

Query: 77  MATLGGLRESQGASNDAEIESLARFAVDEHNKKENALLEFARIIKAKEQVVAGTLHHFTI 136
           MATLGGLRESQGASNDAEIESLARFAV+EHNKKENALLEFAR++KAKEQVVAGTLHHFTI
Sbjct: 1   MATLGGLRESQGASNDAEIESLARFAVEEHNKKENALLEFARVVKAKEQVVAGTLHHFTI 60

Query: 137 EAIDSGKKKLYEAKVWVKPWMNFKELQEFKHTEESHAITSSDLGANKEGHGAGWKEVPVH 196
           EAI++GKKKLY+AKVWVKPWMNFKELQEFKH ++S +IT SDLGA KEGH  GWKEVPVH
Sbjct: 61  EAIEAGKKKLYDAKVWVKPWMNFKELQEFKHADDSPSITPSDLGAIKEGHAPGWKEVPVH 120

Query: 197 DPEVQNAANHALQTIQQRSNSLFPYELQEVSHAKAEVVDDTAKFNLHLKVKRGNKEEKFN 256
           DPEVQ+AANHA++TIQQRSNSLFPYELQE++HAKAEVV+DTAKFNLHLKVKRGNKEE FN
Sbjct: 121 DPEVQDAANHAVKTIQQRSNSLFPYELQEIAHAKAEVVEDTAKFNLHLKVKRGNKEEIFN 180

Query: 257 VEVHKNSQGNYNLNQMGNIEPEFE 281
           VEVHKN++GNYNLNQM NIEPEFE
Sbjct: 181 VEVHKNNEGNYNLNQMNNIEPEFE 204
[Close]
BLAST of Spo04116.1 vs. UniProtKB/TrEMBL
Match: G7LID2_MEDTR (Cysteine proteinase inhibitor OS=Medicago truncatula GN=MTR_8g041410 PE=3 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 3.500e-79
Identity = 150/230 (65.22%), Postives = 184/230 (80.00%), Query Frame = 1 

		  

Query: 48  LIPYLILSIL---IFCHQGSTGSCIDFESEPSMATLGGLRESQGASNDAEIESLARFAVD 107
           ++P+L+       +F    S+G C DF+       LGG+++S  + N  EIESLARFAVD
Sbjct: 7   ILPFLVFFFFFFALFIVLESSGDCSDFDHAQMATPLGGIQDSPSSENSLEIESLARFAVD 66

Query: 108 EHNKKENALLEFARIIKAKEQVVAGTLHHFTIEAIDSGKKKLYEAKVWVKPWMNFKELQE 167
           +HN K+N+LLEFAR++KA+EQVVAGTLHH T+EAID+G+KK+YEAKVWVKPWMNFKEL E
Sbjct: 67  QHNAKQNSLLEFARVVKAREQVVAGTLHHLTLEAIDAGEKKIYEAKVWVKPWMNFKELTE 126

Query: 168 FKHTEESHA--ITSSDLGANKEGHGAGWKEVPVHDPEVQNAANHALQTIQQRSNSLFPYE 227
           FKH  + HA   T+SDLG  K+GH  GW+ VP HDP+VQ+AANHA++TIQQRSNSL PYE
Sbjct: 127 FKHAGDGHAPSFTTSDLGVIKDGHKPGWQSVPAHDPQVQDAANHAIKTIQQRSNSLVPYE 186

Query: 228 LQEVSHAKAEVVDDTAKFNLHLKVKRGNKEEKFNVEVHKNSQGNYNLNQM 273
           L EV+ AKAEV+DDTAKFNL LKVKRG KEEKF VEVHKNS+GN++LNQM
Sbjct: 187 LHEVTDAKAEVIDDTAKFNLLLKVKRGQKEEKFKVEVHKNSEGNFHLNQM 236
[Close]
BLAST of Spo04116.1 vs. ExPASy Swiss-Prot
Match: CYT12_ORYSJ (Cysteine proteinase inhibitor 12 OS=Oryza sativa subsp. japonica GN=Os01g0270100 PE=2 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 1.900e-70
Identity = 126/193 (65.28%), Postives = 165/193 (85.49%), Query Frame = 1 

		  

Query: 80  LGGLRESQGASNDAEIESLARFAVDEHNKKENALLEFARIIKAKEQVVAGTLHHFTIEAI 139
           LGG  ++  A+N  E ++LARFAVDEHNK+ENALLEF R+++AKEQVVAGTLHH T+EA+
Sbjct: 48  LGGAHDAPSAANSVETDALARFAVDEHNKRENALLEFVRVVEAKEQVVAGTLHHLTLEAL 107

Query: 140 DSGKKKLYEAKVWVKPWMNFKELQEFKHTEESHAITSSDLGANKEGHGAGWKEVPVHDPE 199
           ++G+KK+YEAKVWVKPW++FKELQEF++T ++   T++DLGA K GH  GW++VPVHDP 
Sbjct: 108 EAGRKKVYEAKVWVKPWLDFKELQEFRNTGDATTFTNADLGAKKGGHEPGWRDVPVHDPV 167

Query: 200 VQNAANHALQTIQQRSNSLFPYELQEVSHAKAEVVDDTAKFNLHLKVKRGNKEEKFNVEV 259
           V++AA+HA+++IQQRSNSLFPYEL E+  AKAEVV+D AKF++ +K+KRGNKEEKF  EV
Sbjct: 168 VKDAADHAVKSIQQRSNSLFPYELLEIVRAKAEVVEDFAKFDILMKLKRGNKEEKFKAEV 227

Query: 260 HKNSQGNYNLNQM 273
           HKN +G + LNQM
Sbjct: 228 HKNLEGAFVLNQM 240
[Close]
BLAST of Spo04116.1 vs. ExPASy Swiss-Prot
Match: CYT6_ARATH (Cysteine proteinase inhibitor 6 OS=Arabidopsis thaliana GN=CYS6 PE=1 SV=2)

HSP 1 Score: 266.2 bits (679), Expect = 4.200e-70
Identity = 141/238 (59.24%), Postives = 173/238 (72.69%), Query Frame = 1 

		  

Query: 33  KSMLSIFFLCHHSSTLIPYLILSILIFCHQGSTGSCIDFESEPSMATLGGLRESQGASND 92
           +S   +F +    S  I  LI S L FC++              MA +GG+ +     N 
Sbjct: 3   RSRFLLFIVFFSLSLFISSLIASDLGFCNE-------------EMALVGGVGDVPANQNS 62

Query: 93  AEIESLARFAVDEHNKKENALLEFARIIKAKEQVVAGTLHHFTIEAIDSGKKKLYEAKVW 152
            E+ESLARFAVDEHNKKENALLEFAR++KAKEQVVAGTLHH T+E +++G+KKLYEAKVW
Sbjct: 63  GEVESLARFAVDEHNKKENALLEFARVVKAKEQVVAGTLHHLTLEILEAGQKKLYEAKVW 122

Query: 153 VKPWMNFKELQEFKHTEESHAITSSDLGANKEGHGAGWKEVPVHDPEVQNAANHALQTIQ 212
           VKPW+NFKELQEFK   ++ AITSSDLG  +  H +GW+EVP  DPEV++ A  A++TIQ
Sbjct: 123 VKPWLNFKELQEFKPASDAPAITSSDLGCKQGEHESGWREVPGDDPEVKHVAEQAVKTIQ 182

Query: 213 QRSNSLFPYELQEVSHAKAEVVDDTAKFNLHLKVKRGNKEEKFNVEVHKNSQGNYNLN 271
           QRSNSLFPYEL EV HAKAEV  + AK+N+ LK+KRG KEEKF VEVHKN +G  +LN
Sbjct: 183 QRSNSLFPYELLEVVHAKAEVTGEAAKYNMLLKLKRGEKEEKFKVEVHKNHEGALHLN 227
[Close]
BLAST of Spo04116.1 vs. ExPASy Swiss-Prot
Match: CYT7_ARATH (Cysteine proteinase inhibitor 7 OS=Arabidopsis thaliana GN=CYS7 PE=2 SV=2)

HSP 1 Score: 203.8 bits (517), Expect = 2.600e-51
Identity = 105/189 (55.56%), Postives = 142/189 (75.13%), Query Frame = 1 

		  

Query: 80  LGGLRESQGASNDA-EIESLARFAVDEHNKKENALLEFARIIKAKEQVVAGTLHHFTIEA 139
           LGG  +S+   N   EI+ +A FAV EHN++ENA+LE AR++KA EQVVAG L+  T+E 
Sbjct: 45  LGGFSDSKNDWNGGKEIDDIALFAVQEHNRRENAVLELARVLKATEQVVAGKLYRLTLEV 104

Query: 140 IDSGKKKLYEAKVWVKPWMNFKELQEFKHTEESHAITSSDLGANKEGHGAGWKEVPVHDP 199
           I++G+KK+YEAKVWVKPWMNFK+LQEFK+   S   T SDLG   +G+G  W+ V  ++P
Sbjct: 105 IEAGEKKIYEAKVWVKPWMNFKQLQEFKNIIPS--FTISDLGFKPDGNGFDWRSVSTNNP 164

Query: 200 EVQNAANHALQTIQQRSNSLFPYELQEVSHAKAEVVDDTAKFNLHLKVKRGNKEEKFNVE 259
           EVQ AA HA++++QQ+SNSLFPY+L ++  A+A+VV++  KF L LK++RGNK EKF VE
Sbjct: 165 EVQEAAKHAMKSLQQKSNSLFPYKLIDIILARAKVVEERVKFELLLKLERGNKLEKFMVE 224

Query: 260 VHKNSQGNY 268
           V K+  G Y
Sbjct: 225 VMKDQTGKY 231
[Close]
BLAST of Spo04116.1 vs. ExPASy Swiss-Prot
Match: CYTI_VIGUN (Cysteine proteinase inhibitor OS=Vigna unguiculata PE=1 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 5.600e-30
Identity = 63/97 (64.95%), Postives = 79/97 (81.44%), Query Frame = 1 

		  

Query: 77  MATLGGLRESQGASNDAEIESLARFAVDEHNKKENALLEFARIIKAKEQVVAGTLHHFTI 136
           MA LGG R+  G  N  EI+SLARFAV+EHNKK+NALLEF R++ A++QVV+GTL+  T+
Sbjct: 1   MAALGGNRDVAGNQNSLEIDSLARFAVEEHNKKQNALLEFGRVVSAQQQVVSGTLYTITL 60

Query: 137 EAIDSGKKKLYEAKVWVKPWMNFKELQEFKHTEESHA 174
           EA D G+KK+YEAKVW KPW+NFKELQEFKH  ++ A
Sbjct: 61  EAKDGGQKKVYEAKVWEKPWLNFKELQEFKHVGDAPA 97
[Close]
BLAST of Spo04116.1 vs. ExPASy Swiss-Prot
Match: CYT3_ARATH (Cysteine proteinase inhibitor 3 OS=Arabidopsis thaliana GN=CYS3 PE=2 SV=2)

HSP 1 Score: 121.7 bits (304), Expect = 1.300e-26
Identity = 55/89 (61.80%), Postives = 73/89 (82.02%), Query Frame = 1 

		  

Query: 80  LGGLRESQGASNDAEIESLARFAVDEHNKKENALLEFARIIKAKEQVVAGTLHHFTIEAI 139
           LGG+ + +G  N  EIESLARFA+ EHNK++N +LEF +I+KA+EQVVAGT++H T+EA 
Sbjct: 35  LGGVHDLRGNQNSGEIESLARFAIQEHNKQQNKILEFKKIVKAREQVVAGTMYHLTLEAK 94

Query: 140 DSGKKKLYEAKVWVKPWMNFKELQEFKHT 169
           +  + K +EAKVWVKPWMNFK+LQEFK +
Sbjct: 95  EGDQTKNFEAKVWVKPWMNFKQLQEFKES 123
[Close]
BLAST of Spo04116.1 vs. TAIR (Arabidopsis)
Match: AT3G12490.2 (cystatin B)

HSP 1 Score: 266.2 bits (679), Expect = 2.400e-71
Identity = 141/238 (59.24%), Postives = 173/238 (72.69%), Query Frame = 1 

		  

Query: 33  KSMLSIFFLCHHSSTLIPYLILSILIFCHQGSTGSCIDFESEPSMATLGGLRESQGASND 92
           +S   +F +    S  I  LI S L FC++              MA +GG+ +     N 
Sbjct: 3   RSRFLLFIVFFSLSLFISSLIASDLGFCNE-------------EMALVGGVGDVPANQNS 62

Query: 93  AEIESLARFAVDEHNKKENALLEFARIIKAKEQVVAGTLHHFTIEAIDSGKKKLYEAKVW 152
            E+ESLARFAVDEHNKKENALLEFAR++KAKEQVVAGTLHH T+E +++G+KKLYEAKVW
Sbjct: 63  GEVESLARFAVDEHNKKENALLEFARVVKAKEQVVAGTLHHLTLEILEAGQKKLYEAKVW 122

Query: 153 VKPWMNFKELQEFKHTEESHAITSSDLGANKEGHGAGWKEVPVHDPEVQNAANHALQTIQ 212
           VKPW+NFKELQEFK   ++ AITSSDLG  +  H +GW+EVP  DPEV++ A  A++TIQ
Sbjct: 123 VKPWLNFKELQEFKPASDAPAITSSDLGCKQGEHESGWREVPGDDPEVKHVAEQAVKTIQ 182

Query: 213 QRSNSLFPYELQEVSHAKAEVVDDTAKFNLHLKVKRGNKEEKFNVEVHKNSQGNYNLN 271
           QRSNSLFPYEL EV HAKAEV  + AK+N+ LK+KRG KEEKF VEVHKN +G  +LN
Sbjct: 183 QRSNSLFPYELLEVVHAKAEVTGEAAKYNMLLKLKRGEKEEKFKVEVHKNHEGALHLN 227
[Close]
BLAST of Spo04116.1 vs. TAIR (Arabidopsis)
Match: AT5G05110.1 (Cystatin/monellin family protein)

HSP 1 Score: 203.8 bits (517), Expect = 1.400e-52
Identity = 105/189 (55.56%), Postives = 142/189 (75.13%), Query Frame = 1 

		  

Query: 80  LGGLRESQGASNDA-EIESLARFAVDEHNKKENALLEFARIIKAKEQVVAGTLHHFTIEA 139
           LGG  +S+   N   EI+ +A FAV EHN++ENA+LE AR++KA EQVVAG L+  T+E 
Sbjct: 45  LGGFSDSKNDWNGGKEIDDIALFAVQEHNRRENAVLELARVLKATEQVVAGKLYRLTLEV 104

Query: 140 IDSGKKKLYEAKVWVKPWMNFKELQEFKHTEESHAITSSDLGANKEGHGAGWKEVPVHDP 199
           I++G+KK+YEAKVWVKPWMNFK+LQEFK+   S   T SDLG   +G+G  W+ V  ++P
Sbjct: 105 IEAGEKKIYEAKVWVKPWMNFKQLQEFKNIIPS--FTISDLGFKPDGNGFDWRSVSTNNP 164

Query: 200 EVQNAANHALQTIQQRSNSLFPYELQEVSHAKAEVVDDTAKFNLHLKVKRGNKEEKFNVE 259
           EVQ AA HA++++QQ+SNSLFPY+L ++  A+A+VV++  KF L LK++RGNK EKF VE
Sbjct: 165 EVQEAAKHAMKSLQQKSNSLFPYKLIDIILARAKVVEERVKFELLLKLERGNKLEKFMVE 224

Query: 260 VHKNSQGNY 268
           V K+  G Y
Sbjct: 225 VMKDQTGKY 231
[Close]
BLAST of Spo04116.1 vs. TAIR (Arabidopsis)
Match: AT2G40880.1 (cystatin A)

HSP 1 Score: 121.7 bits (304), Expect = 7.200e-28
Identity = 55/89 (61.80%), Postives = 73/89 (82.02%), Query Frame = 1 

		  

Query: 80  LGGLRESQGASNDAEIESLARFAVDEHNKKENALLEFARIIKAKEQVVAGTLHHFTIEAI 139
           LGG+ + +G  N  EIESLARFA+ EHNK++N +LEF +I+KA+EQVVAGT++H T+EA 
Sbjct: 35  LGGVHDLRGNQNSGEIESLARFAIQEHNKQQNKILEFKKIVKAREQVVAGTMYHLTLEAK 94

Query: 140 DSGKKKLYEAKVWVKPWMNFKELQEFKHT 169
           +  + K +EAKVWVKPWMNFK+LQEFK +
Sbjct: 95  EGDQTKNFEAKVWVKPWMNFKQLQEFKES 123
[Close]
BLAST of Spo04116.1 vs. TAIR (Arabidopsis)
Match: AT5G12140.1 (cystatin-1)

HSP 1 Score: 105.9 bits (263), Expect = 4.100e-23
Identity = 49/91 (53.85%), Postives = 64/91 (70.33%), Query Frame = 1 

		  

Query: 80  LGGLRESQGASNDAEIESLARFAVDEHNKKENALLEFARIIKAKEQVVAGTLHHFTIEAI 139
           +GG+R+    +ND ++ESLARFAVDEHNK EN  LE+ R++ AK QVVAGT+HH T+E  
Sbjct: 10  VGGVRDIDANANDLQVESLARFAVDEHNKNENLTLEYKRLLGAKTQVVAGTMHHLTVEVA 69

Query: 140 DSGKKKLYEAKVWVKPWMNFKELQEFKHTEE 171
           D    K+YEAKV  K W N K+L+ F H  +
Sbjct: 70  DGETNKVYEAKVLEKAWENLKQLESFNHLHD 100
[Close]
BLAST of Spo04116.1 vs. TAIR (Arabidopsis)
Match: AT4G16500.1 (Cystatin/monellin superfamily protein)

HSP 1 Score: 59.7 bits (143), Expect = 3.400e-9
Identity = 34/89 (38.20%), Postives = 53/89 (59.55%), Query Frame = 1 

		  

Query: 81  GGLRESQGASN--DAEIESLARFAVDEHNKKENALLEFARIIKAKEQVVAGTLHHFTIEA 140
           GGL   +   N  D ++ ++A++A++EHNK+    L F ++++   QVV+GT +   I A
Sbjct: 27  GGLGSRKPIKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTTQVVSGTKYDLKIAA 86

Query: 141 IDSGKK-KLYEAKVWVKPWMNFKELQEFK 167
            D G K K YEA V  K W++ K L+ FK
Sbjct: 87  KDGGGKIKNYEAVVVEKLWLHSKSLESFK 115
The following BLAST results are available for this feature:
BLAST of Spo04116.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902212731|gb|KNA17069.1|6.6e-156100.hypothetical protein SOVF_0834... [more]
gi|902212732|gb|KNA17070.1|4.7e-15499.6hypothetical protein SOVF_0834... [more]
gi|110748606|gb|ABG89856.1|8.7e-10880.2cystatin [Amaranthus hypochond... [more]
gi|731320021|ref|XP_010670580.1|3.8e-10385.5PREDICTED: cysteine proteinase... [more]
gi|870866005|gb|KMT17034.1|8.2e-9887.7hypothetical protein BVRB_2g04... [more]
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BLAST of Spo04116.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9RDK0_SPIOL4.6e-156100.Cysteine proteinase inhibitor ... [more]
A0A0K9RC70_SPIOL3.3e-15499.6Cysteine proteinase inhibitor ... [more]
Q0GPA4_AMAHP6.1e-10880.2Cysteine proteinase inhibitor ... [more]
A0A0J8CY51_BETVU5.7e-9887.7Cysteine proteinase inhibitor ... [more]
G7LID2_MEDTR3.5e-7965.2Cysteine proteinase inhibitor ... [more]
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BLAST of Spo04116.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5
Match NameE-valueIdentityDescription
CYT12_ORYSJ1.9e-7065.2Cysteine proteinase inhibitor ... [more]
CYT6_ARATH4.2e-7059.2Cysteine proteinase inhibitor ... [more]
CYT7_ARATH2.6e-5155.5Cysteine proteinase inhibitor ... [more]
CYTI_VIGUN5.6e-3064.9Cysteine proteinase inhibitor ... [more]
CYT3_ARATH1.3e-2661.8Cysteine proteinase inhibitor ... [more]
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BLAST of Spo04116.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5
Match NameE-valueIdentityDescription
AT3G12490.22.4e-7159.2cystatin B[more]
AT5G05110.11.4e-5255.5Cystatin/monellin family prote... [more]
AT2G40880.17.2e-2861.8cystatin A[more]
AT5G12140.14.1e-2353.8cystatin-1[more]
AT4G16500.13.4e-938.2Cystatin/monellin superfamily ... [more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000010Cystatin domainPFAMPF00031Cystatincoord: 189..258
score: 2.
IPR000010Cystatin domainSMARTSM00043CY_4coord: 78..168
score: 1.4
IPR018073Proteinase inhibitor I25, cystatin, conserved sitePROSITEPS00287CYSTATINcoord: 124..137
scor
IPR020381Proteinase inhibitor I25, cystatin, conserved regionPRODOMPD001231coord: 93..166
score: 2.0
IPR020381Proteinase inhibitor I25, cystatin, conserved regionPFAMPF16845SQAPIcoord: 91..167
score: 8.8
IPR027214CystatinPANTHERPTHR11413CYSTATIN FAMILY MEMBERcoord: 49..172
score: 5.3E-61coord: 249..278
score: 5.3
NoneNo IPR availableGENE3D3.10.450.10coord: 80..166
score: 1.5E-34coord: 189..261
score: 8.6
NoneNo IPR availablePANTHERPTHR11413:SF55CYSTEINE PROTEINASE INHIBITOR 6coord: 49..172
score: 5.3E-61coord: 249..278
score: 5.3
NoneNo IPR availableunknownSSF54403Cystatin/monellincoord: 187..267
score: 1.31E-17coord: 78..169
score: 1.68

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
biological_process GO:0006629 lipid metabolic process
biological_process GO:0055085 transmembrane transport
biological_process GO:0019243 methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione
biological_process GO:0015886 heme transport
biological_process GO:0006007 glucose catabolic process
biological_process GO:0008152 metabolic process
biological_process GO:0006468 protein phosphorylation
biological_process GO:0006508 proteolysis
biological_process GO:0009069 serine family amino acid metabolic process
biological_process GO:0009987 cellular process
biological_process GO:0009414 response to water deprivation
biological_process GO:0006979 response to oxidative stress
biological_process GO:0009409 response to cold
biological_process GO:0010951 negative regulation of endopeptidase activity
biological_process GO:0006972 hyperosmotic response
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0005829 cytosol
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0005576 extracellular region
molecular_function GO:0015439 heme-transporting ATPase activity
molecular_function GO:0008235 metalloexopeptidase activity
molecular_function GO:0016805 dipeptidase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0008430 selenium binding
molecular_function GO:0005515 protein binding
molecular_function GO:0004177 aminopeptidase activity
molecular_function GO:0030145 manganese ion binding
molecular_function GO:0005524 ATP binding
molecular_function GO:0050897 cobalt ion binding
molecular_function GO:0004869 cysteine-type endopeptidase inhibitor activity
molecular_function GO:0042393 histone binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0050505 hydroquinone glucosyltransferase activity
molecular_function GO:0008233 peptidase activity
molecular_function GO:0004190 aspartic-type endopeptidase activity
molecular_function GO:0008270 zinc ion binding
RNA-Seq Expression