Spo04307 (gene)

Overview
NameSpo04307
Typegene
OrganismSpinacia oleracea (Spinach)
Description12-oxophytodienoate reductase-like protein
LocationSpoScf_00648 : 236542 .. 236964 (-)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTACACCAACGATTCCCATGACTCTGACCCACTTTCCCTGGGCTTAGCAGTGGTAGAGAGACTTAACAAAAGGCAAGAAGAAATAGGGCTTGAACTCCCTTACTTGCAAATTCAAGCAGGAACGCTTACCCACGGCATTGGTGAAACAGAAGTTGAGCTTCTCAGAGAGCTCAGGAGAGCATACCAAGGAACCTTCATGATCAGTGGAGGATTCAACAAAGAGCTGGGGATGAAGGCCATTGCTGAAGATGATGCTGATCTCGTTGCTTATGCCCGTTTTTTCCTCTCAAACCCTGATTTGGTTCGCCGGTTTGAGATCGACGCACCATTGAATGATTATGATGCTGCTACATTCTATACCCACGATCCTGTAGTTGGTTACAGTGATTACCCCTCCTTGGATGAAAATAGTGAAACCTGA

mRNA sequence

ATGTACACCAACGATTCCCATGACTCTGACCCACTTTCCCTGGGCTTAGCAGTGGTAGAGAGACTTAACAAAAGGCAAGAAGAAATAGGGCTTGAACTCCCTTACTTGCAAATTCAAGCAGGAACGCTTACCCACGGCATTGGTGAAACAGAAGTTGAGCTTCTCAGAGAGCTCAGGAGAGCATACCAAGGAACCTTCATGATCAGTGGAGGATTCAACAAAGAGCTGGGGATGAAGGCCATTGCTGAAGATGATGCTGATCTCGTTGCTTATGCCCGTTTTTTCCTCTCAAACCCTGATTTGGTTCGCCGGTTTGAGATCGACGCACCATTGAATGATTATGATGCTGCTACATTCTATACCCACGATCCTGTAGTTGGTTACAGTGATTACCCCTCCTTGGATGAAAATAGTGAAACCTGA

Coding sequence (CDS)

ATGTACACCAACGATTCCCATGACTCTGACCCACTTTCCCTGGGCTTAGCAGTGGTAGAGAGACTTAACAAAAGGCAAGAAGAAATAGGGCTTGAACTCCCTTACTTGCAAATTCAAGCAGGAACGCTTACCCACGGCATTGGTGAAACAGAAGTTGAGCTTCTCAGAGAGCTCAGGAGAGCATACCAAGGAACCTTCATGATCAGTGGAGGATTCAACAAAGAGCTGGGGATGAAGGCCATTGCTGAAGATGATGCTGATCTCGTTGCTTATGCCCGTTTTTTCCTCTCAAACCCTGATTTGGTTCGCCGGTTTGAGATCGACGCACCATTGAATGATTATGATGCTGCTACATTCTATACCCACGATCCTGTAGTTGGTTACAGTGATTACCCCTCCTTGGATGAAAATAGTGAAACCTGA

Protein sequence

MYTNDSHDSDPLSLGLAVVERLNKRQEEIGLELPYLQIQAGTLTHGIGETEVELLRELRRAYQGTFMISGGFNKELGMKAIAEDDADLVAYARFFLSNPDLVRRFEIDAPLNDYDAATFYTHDPVVGYSDYPSLDENSET
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo04307.1Spo04307.1mRNA


Homology
[Close]
BLAST of Spo04307.1 vs. NCBI nr
Match: gi|902140180|gb|KNA04620.1| (hypothetical protein SOVF_197850 [Spinacia oleracea])

HSP 1 Score: 286.2 bits (731), Expect = 3.100e-74
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 1 

		  

Query: 1   MYTNDSHDSDPLSLGLAVVERLNKRQEEIGLELPYLQIQAGTLTHGIGETEVELLRELRR 60
           MYTNDSHDSDPLSLGLAVVERLNKRQEEIGLELPYLQIQAGTLTHGIGETEVELLRELRR
Sbjct: 221 MYTNDSHDSDPLSLGLAVVERLNKRQEEIGLELPYLQIQAGTLTHGIGETEVELLRELRR 280

Query: 61  AYQGTFMISGGFNKELGMKAIAEDDADLVAYARFFLSNPDLVRRFEIDAPLNDYDAATFY 120
           AYQGTFMISGGFNKELGMKAIAEDDADLVAYARFFLSNPDLVRRFEIDAPLNDYDAATFY
Sbjct: 281 AYQGTFMISGGFNKELGMKAIAEDDADLVAYARFFLSNPDLVRRFEIDAPLNDYDAATFY 340

Query: 121 THDPVVGYSDYPSLDENSET 141
           THDPVVGYSDYPSLDENSET
Sbjct: 341 THDPVVGYSDYPSLDENSET 360
[Close]
BLAST of Spo04307.1 vs. NCBI nr
Match: gi|731338486|ref|XP_010680356.1| (PREDICTED: artemisinic aldehyde Delta(11(13)) reductase-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 237.7 bits (605), Expect = 1.300e-59
Identity = 112/135 (82.96%), Postives = 125/135 (92.59%), Query Frame = 1 

		  

Query: 1   MYTNDSHDSDPLSLGLAVVERLNKRQEEIGLELPYLQIQAGTLTHGIGETEVELLRELRR 60
           MYT D+HDSDPL+LGLAVV+RLNK+QEEIG +LPYLQIQAGTL+HGI E+E ++LRELRR
Sbjct: 253 MYTQDAHDSDPLALGLAVVKRLNKQQEEIGFKLPYLQIQAGTLSHGISESEAQVLRELRR 312

Query: 61  AYQGTFMISGGFNKELGMKAIAEDDADLVAYARFFLSNPDLVRRFEIDAPLNDYDAATFY 120
           AY GTFMISGGFNK LGMKAIAE DADL+AYARFFLSNPDLVRRFEIDAPLN +DAATFY
Sbjct: 313 AYHGTFMISGGFNKALGMKAIAEQDADLIAYARFFLSNPDLVRRFEIDAPLNAFDAATFY 372

Query: 121 THDPVVGYSDYPSLD 136
           THDPVVGY+DY  +D
Sbjct: 373 THDPVVGYTDYTFID 387
[Close]
BLAST of Spo04307.1 vs. NCBI nr
Match: gi|731338484|ref|XP_010680354.1| (PREDICTED: artemisinic aldehyde Delta(11(13)) reductase-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 233.4 bits (594), Expect = 2.400e-58
Identity = 114/140 (81.43%), Postives = 127/140 (90.71%), Query Frame = 1 

		  

Query: 1   MYTNDSHDSDPLSLGLAVVERLNKRQEEI-GLELPYLQIQAGTLTHGIGETEVELLRELR 60
           +Y  D+HDSDPL+LGLAVVERLNK QE+  GL+L +LQIQAGTLTHGIGE E +LLRELR
Sbjct: 251 LYHQDAHDSDPLALGLAVVERLNKLQEDQQGLKLSHLQIQAGTLTHGIGEEEAKLLRELR 310

Query: 61  RAYQGTFMISGGFNKELGMKAIAEDDADLVAYARFFLSNPDLVRRFEIDAPLNDYDAATF 120
           RAY+GTFMISGGFNKELGMKA+AEDDADL+AYAR FLSNPDLVRRF +DAPLN YDAATF
Sbjct: 311 RAYKGTFMISGGFNKELGMKAVAEDDADLIAYARLFLSNPDLVRRFVMDAPLNVYDAATF 370

Query: 121 YTHDPVVGYSDYPSLDENSE 140
           YTHDPVVGY+DYP LDE S+
Sbjct: 371 YTHDPVVGYTDYPFLDETSK 390
[Close]
BLAST of Spo04307.1 vs. NCBI nr
Match: gi|902140181|gb|KNA04621.1| (hypothetical protein SOVF_197860 [Spinacia oleracea])

HSP 1 Score: 223.4 bits (568), Expect = 2.500e-55
Identity = 108/139 (77.70%), Postives = 121/139 (87.05%), Query Frame = 1 

		  

Query: 1   MYTNDSHDSDPLSLGLAVVERLNKRQEEIGLELPYLQIQAGTLTHGIGETEVELLRELRR 60
           +Y  D+HDS+ L+LGLAVVE LNK QEE GL+L YLQIQAGT T GI E E E LRELRR
Sbjct: 251 LYHQDAHDSNQLALGLAVVEGLNKLQEEQGLKLCYLQIQAGTFTRGITEKEAEFLRELRR 310

Query: 61  AYQGTFMISGGFNKELGMKAIAEDDADLVAYARFFLSNPDLVRRFEIDAPLNDYDAATFY 120
           AY GTFM+SGGFNKELGMKAIAE DAD++AYAR FLSNPDLVRRFEI APLN+YDAATFY
Sbjct: 311 AYHGTFMVSGGFNKELGMKAIAEGDADVIAYARLFLSNPDLVRRFEIGAPLNEYDAATFY 370

Query: 121 THDPVVGYSDYPSLDENSE 140
           THDPV+GY+DYP LDE+S+
Sbjct: 371 THDPVLGYTDYPFLDESSQ 389
[Close]
BLAST of Spo04307.1 vs. NCBI nr
Match: gi|702304286|ref|XP_010049528.1| (PREDICTED: 12-oxophytodienoate reductase 3-like [Eucalyptus grandis])

HSP 1 Score: 171.0 bits (432), Expect = 1.500e-39
Identity = 89/145 (61.38%), Postives = 105/145 (72.41%), Query Frame = 1 

		  

Query: 4   NDSHDSDPLSLGLAVVERLNKRQEEIGLELPYLQIQA------GTLTHGIGETEVE--LL 63
           ND+ D DPLSLGLAV+ERLNK Q EIG +L YL +        GT  +G  E + E  L+
Sbjct: 238 NDATDPDPLSLGLAVIERLNKFQNEIGSKLVYLHVTQPRFTANGTKNYGEQEEDEEARLI 297

Query: 64  RELRRAYQGTFMISGGFNKELGMKAIAEDDADLVAYARFFLSNPDLVRRFEIDAPLNDYD 123
           R  RRAYQGTFM+SGGF +ELGMK +A  DADL++Y R F+SNPDLV RF++DAPLN Y+
Sbjct: 298 RTWRRAYQGTFMLSGGFTRELGMKVLAGGDADLISYGRLFISNPDLVYRFKVDAPLNGYN 357

Query: 124 AATFYTHDPVVGYSDYPSLD-ENSE 140
             TFYT DPVVGY DYP LD EN E
Sbjct: 358 RETFYTQDPVVGYIDYPFLDQENGE 382
[Close]
BLAST of Spo04307.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QBJ7_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_197850 PE=4 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 2.200e-74
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 1 

		  

Query: 1   MYTNDSHDSDPLSLGLAVVERLNKRQEEIGLELPYLQIQAGTLTHGIGETEVELLRELRR 60
           MYTNDSHDSDPLSLGLAVVERLNKRQEEIGLELPYLQIQAGTLTHGIGETEVELLRELRR
Sbjct: 221 MYTNDSHDSDPLSLGLAVVERLNKRQEEIGLELPYLQIQAGTLTHGIGETEVELLRELRR 280

Query: 61  AYQGTFMISGGFNKELGMKAIAEDDADLVAYARFFLSNPDLVRRFEIDAPLNDYDAATFY 120
           AYQGTFMISGGFNKELGMKAIAEDDADLVAYARFFLSNPDLVRRFEIDAPLNDYDAATFY
Sbjct: 281 AYQGTFMISGGFNKELGMKAIAEDDADLVAYARFFLSNPDLVRRFEIDAPLNDYDAATFY 340

Query: 121 THDPVVGYSDYPSLDENSET 141
           THDPVVGYSDYPSLDENSET
Sbjct: 341 THDPVVGYSDYPSLDENSET 360
[Close]
BLAST of Spo04307.1 vs. UniProtKB/TrEMBL
Match: A0A0J8C6V0_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_6g133820 PE=4 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 8.800e-60
Identity = 112/135 (82.96%), Postives = 125/135 (92.59%), Query Frame = 1 

		  

Query: 1   MYTNDSHDSDPLSLGLAVVERLNKRQEEIGLELPYLQIQAGTLTHGIGETEVELLRELRR 60
           MYT D+HDSDPL+LGLAVV+RLNK+QEEIG +LPYLQIQAGTL+HGI E+E ++LRELRR
Sbjct: 253 MYTQDAHDSDPLALGLAVVKRLNKQQEEIGFKLPYLQIQAGTLSHGISESEAQVLRELRR 312

Query: 61  AYQGTFMISGGFNKELGMKAIAEDDADLVAYARFFLSNPDLVRRFEIDAPLNDYDAATFY 120
           AY GTFMISGGFNK LGMKAIAE DADL+AYARFFLSNPDLVRRFEIDAPLN +DAATFY
Sbjct: 313 AYHGTFMISGGFNKALGMKAIAEQDADLIAYARFFLSNPDLVRRFEIDAPLNAFDAATFY 372

Query: 121 THDPVVGYSDYPSLD 136
           THDPVVGY+DY  +D
Sbjct: 373 THDPVVGYTDYTFID 387
[Close]
BLAST of Spo04307.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CBA4_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_6g133830 PE=4 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 1.700e-58
Identity = 114/140 (81.43%), Postives = 127/140 (90.71%), Query Frame = 1 

		  

Query: 1   MYTNDSHDSDPLSLGLAVVERLNKRQEEI-GLELPYLQIQAGTLTHGIGETEVELLRELR 60
           +Y  D+HDSDPL+LGLAVVERLNK QE+  GL+L +LQIQAGTLTHGIGE E +LLRELR
Sbjct: 251 LYHQDAHDSDPLALGLAVVERLNKLQEDQQGLKLSHLQIQAGTLTHGIGEEEAKLLRELR 310

Query: 61  RAYQGTFMISGGFNKELGMKAIAEDDADLVAYARFFLSNPDLVRRFEIDAPLNDYDAATF 120
           RAY+GTFMISGGFNKELGMKA+AEDDADL+AYAR FLSNPDLVRRF +DAPLN YDAATF
Sbjct: 311 RAYKGTFMISGGFNKELGMKAVAEDDADLIAYARLFLSNPDLVRRFVMDAPLNVYDAATF 370

Query: 121 YTHDPVVGYSDYPSLDENSE 140
           YTHDPVVGY+DYP LDE S+
Sbjct: 371 YTHDPVVGYTDYPFLDETSK 390
[Close]
BLAST of Spo04307.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QDM9_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_197860 PE=4 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 1.700e-55
Identity = 108/139 (77.70%), Postives = 121/139 (87.05%), Query Frame = 1 

		  

Query: 1   MYTNDSHDSDPLSLGLAVVERLNKRQEEIGLELPYLQIQAGTLTHGIGETEVELLRELRR 60
           +Y  D+HDS+ L+LGLAVVE LNK QEE GL+L YLQIQAGT T GI E E E LRELRR
Sbjct: 251 LYHQDAHDSNQLALGLAVVEGLNKLQEEQGLKLCYLQIQAGTFTRGITEKEAEFLRELRR 310

Query: 61  AYQGTFMISGGFNKELGMKAIAEDDADLVAYARFFLSNPDLVRRFEIDAPLNDYDAATFY 120
           AY GTFM+SGGFNKELGMKAIAE DAD++AYAR FLSNPDLVRRFEI APLN+YDAATFY
Sbjct: 311 AYHGTFMVSGGFNKELGMKAIAEGDADVIAYARLFLSNPDLVRRFEIGAPLNEYDAATFY 370

Query: 121 THDPVVGYSDYPSLDENSE 140
           THDPV+GY+DYP LDE+S+
Sbjct: 371 THDPVLGYTDYPFLDESSQ 389
[Close]
BLAST of Spo04307.1 vs. UniProtKB/TrEMBL
Match: A0A059CVA1_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_C03542 PE=4 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 1.700e-39
Identity = 84/142 (59.15%), Postives = 105/142 (73.94%), Query Frame = 1 

		  

Query: 4   NDSHDSDPLSLGLAVVERLNKRQEEIGLELPYLQIQ------AGTLTHGIGETEVELLRE 63
           ND+ DSDPL LGLAV++RLNK Q+E+G +L YL +       A T   G  E E  L+R 
Sbjct: 243 NDATDSDPLGLGLAVIKRLNKFQDELGSKLAYLHVTRPRFMAAPTEEEGEDE-EARLMRA 302

Query: 64  LRRAYQGTFMISGGFNKELGMKAIAEDDADLVAYARFFLSNPDLVRRFEIDAPLNDYDAA 123
            RRAYQGTFM +GGF +ELGMKA+A+ DADL++Y R F+SNPDLVRRF++DAPLN Y+  
Sbjct: 303 WRRAYQGTFMSTGGFTRELGMKAVADGDADLISYGRLFISNPDLVRRFKVDAPLNSYNRE 362

Query: 124 TFYTHDPVVGYSDYPSLDENSE 140
           TFY  DPVVGY DYP LD+ ++
Sbjct: 363 TFYIQDPVVGYIDYPFLDQENK 383
[Close]
BLAST of Spo04307.1 vs. ExPASy Swiss-Prot
Match: DBR2_ARTAN (Artemisinic aldehyde Delta(11(13)) reductase OS=Artemisia annua GN=DBR2 PE=1 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 1.600e-38
Identity = 80/145 (55.17%), Postives = 104/145 (71.72%), Query Frame = 1 

		  

Query: 5   DSHDSDPLSLGLAVVERLNKRQEEIGLELPYLQIQAGTLT-HGIGETEV----------E 64
           D+ DSDP SLGLAV+ERLNK Q ++G  L YL +     T  G G+TE           +
Sbjct: 241 DAMDSDPRSLGLAVIERLNKLQFKLGSRLAYLHVTQPRYTADGHGQTEAGANGSEEEVAQ 300

Query: 65  LLRELRRAYQGTFMISGGFNKELGMKAIAEDDADLVAYARFFLSNPDLVRRFEIDAPLND 124
           L++  R AY GTF+  GG+ +ELG++A+A+ DADLVA+ R+F+SNPDLV R +++APLN 
Sbjct: 301 LMKTWRGAYVGTFICCGGYTRELGLQAVAQGDADLVAFGRYFVSNPDLVLRLKLNAPLNR 360

Query: 125 YDAATFYTHDPVVGYSDYPSLDENS 139
           YD ATFYTHDPVVGY+DYPSLD+ S
Sbjct: 361 YDRATFYTHDPVVGYTDYPSLDKGS 385
[Close]
BLAST of Spo04307.1 vs. ExPASy Swiss-Prot
Match: OPR3_SOLLC (12-oxophytodienoate reductase 3 OS=Solanum lycopersicum GN=OPR3 PE=1 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 1.400e-37
Identity = 82/144 (56.94%), Postives = 101/144 (70.14%), Query Frame = 1 

		  

Query: 5   DSHDSDPLSLGLAVVERLNKRQEEIGLELPYL-----------QIQAGTLTHGIGETEVE 64
           D+ DS+PLSLGLAVVERLNK Q   G +L YL           Q +AG L  G  E E  
Sbjct: 246 DAMDSNPLSLGLAVVERLNKIQLHSGSKLAYLHVTQPRYVAYGQTEAGRL--GSEEEEAR 305

Query: 65  LLRELRRAYQGTFMISGGFNKELGMKAIAEDDADLVAYARFFLSNPDLVRRFEIDAPLND 124
           L+R LR AYQGTF+ SGG+ +ELG++A+A+ DADLV+Y R F+SNPDLV R +++APLN 
Sbjct: 306 LMRTLRNAYQGTFICSGGYTRELGIEAVAQGDADLVSYGRLFISNPDLVMRIKLNAPLNK 365

Query: 125 YDAATFYTHDPVVGYSDYPSLDEN 138
           Y+  TFYT DPVVGY+DYP L  N
Sbjct: 366 YNRKTFYTQDPVVGYTDYPFLQGN 387
[Close]
BLAST of Spo04307.1 vs. ExPASy Swiss-Prot
Match: OPR3_ARATH (12-oxophytodienoate reductase 3 OS=Arabidopsis thaliana GN=OPR3 PE=1 SV=2)

HSP 1 Score: 154.5 bits (389), Expect = 8.800e-37
Identity = 79/139 (56.83%), Postives = 95/139 (68.35%), Query Frame = 1 

		  

Query: 5   DSHDSDPLSLGLAVVERLNKRQEEIGLELPYLQI---------QAGTLTHGIGETEVELL 64
           D+ DSDPLSLGLAVV  LNK Q   G +L YL +         Q  +   G  E E +L+
Sbjct: 247 DATDSDPLSLGLAVVGMLNKLQGVNGSKLAYLHVTQPRYHAYGQTESGRQGSDEEEAKLM 306

Query: 65  RELRRAYQGTFMISGGFNKELGMKAIAEDDADLVAYARFFLSNPDLVRRFEIDAPLNDYD 124
           + LR AY GTFM SGGFNKELGM+A+ + DADLV+Y R F++NPDLV RF+ID  LN Y+
Sbjct: 307 KSLRMAYNGTFMSSGGFNKELGMQAVQQGDADLVSYGRLFIANPDLVSRFKIDGELNKYN 366

Query: 125 AATFYTHDPVVGYSDYPSL 135
             TFYT DPVVGY+DYP L
Sbjct: 367 RKTFYTQDPVVGYTDYPFL 385
[Close]
BLAST of Spo04307.1 vs. ExPASy Swiss-Prot
Match: OPR7_ORYSJ (12-oxophytodienoate reductase 7 OS=Oryza sativa subsp. japonica GN=OPR7 PE=2 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 3.700e-35
Identity = 75/141 (53.19%), Postives = 94/141 (66.67%), Query Frame = 1 

		  

Query: 5   DSHDSDPLSLGLAVVERLNKRQEEIGLELPYLQIQAGTLT---------HGIGETEVELL 64
           D++DSDP+ LG+AVVERLN  Q++ G  L YL +     T         HG  E E  L+
Sbjct: 250 DAYDSDPIKLGMAVVERLNALQQQSG-RLAYLHVTQPRYTAYGQTESGQHGSAEEESRLM 309

Query: 65  RELRRAYQGTFMISGGFNKELGMKAIAEDDADLVAYARFFLSNPDLVRRFEIDAPLNDYD 124
           R LR  YQGTFM SGG+ +ELG++A+   DADLV+Y R F+SNPDLV RF ++A LN Y 
Sbjct: 310 RTLRGTYQGTFMCSGGYTRELGLEAVESGDADLVSYGRLFISNPDLVERFRLNAGLNKYV 369

Query: 125 AATFYTHDPVVGYSDYPSLDE 137
             TFYT DPVVGY+DYP L +
Sbjct: 370 RKTFYTPDPVVGYTDYPFLGQ 389
[Close]
BLAST of Spo04307.1 vs. ExPASy Swiss-Prot
Match: OPR6_ORYSJ (Putative 12-oxophytodienoate reductase 6 OS=Oryza sativa subsp. japonica GN=OPR6 PE=2 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 4.000e-29
Identity = 67/137 (48.91%), Postives = 92/137 (67.15%), Query Frame = 1 

		  

Query: 5   DSHDSDPLSLGLAVVERLNKRQEEIGLEL--PYLQIQAGT--LTHGIGETEVELLRELRR 64
           D  DSDP +LG  +VE+LNK ++ +   +  P + I  G   + HG        L   R+
Sbjct: 250 DCVDSDPEALGSYMVEQLNKHEDFLYCHMVEPRMAIVDGRRQIQHG--------LLPFRK 309

Query: 65  AYQGTFMISGGFNKELGMKAIAEDDADLVAYARFFLSNPDLVRRFEIDAPLNDYDAATFY 124
            + GTF+ +GG+++E G KA+A+  ADLVAY R FL+NPDL +RFE+DAP+N+YD  TFY
Sbjct: 310 QFNGTFIAAGGYDREEGNKAVADGYADLVAYGRLFLANPDLPKRFELDAPMNNYDRNTFY 369

Query: 125 THDPVVGYSDYPSLDEN 138
           T DPVVGY+DYP LDE+
Sbjct: 370 TQDPVVGYTDYPFLDEH 378
[Close]
BLAST of Spo04307.1 vs. TAIR (Arabidopsis)
Match: AT2G06050.2 (oxophytodienoate-reductase 3)

HSP 1 Score: 154.5 bits (389), Expect = 5.000e-38
Identity = 79/139 (56.83%), Postives = 95/139 (68.35%), Query Frame = 1 

		  

Query: 5   DSHDSDPLSLGLAVVERLNKRQEEIGLELPYLQI---------QAGTLTHGIGETEVELL 64
           D+ DSDPLSLGLAVV  LNK Q   G +L YL +         Q  +   G  E E +L+
Sbjct: 247 DATDSDPLSLGLAVVGMLNKLQGVNGSKLAYLHVTQPRYHAYGQTESGRQGSDEEEAKLM 306

Query: 65  RELRRAYQGTFMISGGFNKELGMKAIAEDDADLVAYARFFLSNPDLVRRFEIDAPLNDYD 124
           + LR AY GTFM SGGFNKELGM+A+ + DADLV+Y R F++NPDLV RF+ID  LN Y+
Sbjct: 307 KSLRMAYNGTFMSSGGFNKELGMQAVQQGDADLVSYGRLFIANPDLVSRFKIDGELNKYN 366

Query: 125 AATFYTHDPVVGYSDYPSL 135
             TFYT DPVVGY+DYP L
Sbjct: 367 RKTFYTQDPVVGYTDYPFL 385
[Close]
BLAST of Spo04307.1 vs. TAIR (Arabidopsis)
Match: AT1G76690.1 (12-oxophytodienoate reductase 2)

HSP 1 Score: 120.9 bits (302), Expect = 6.100e-28
Identity = 63/134 (47.01%), Postives = 91/134 (67.91%), Query Frame = 1 

		  

Query: 5   DSHDSDPLSLGLAVVERLNKRQEEIGLELPYLQIQAGTLTHGIGETEVELLRELRRAYQG 64
           +S D++P +LGL +VE LNK     G+   ++ I+    T G        L  +R A++G
Sbjct: 246 ESGDTNPEALGLYMVESLNK----YGILYCHM-IEPRMKTVGEIAACSHTLMPMREAFKG 305

Query: 65  TFMISGGFNKELGMKAIAEDDADLVAYARFFLSNPDLVRRFEIDAPLNDYDAATFYTHDP 124
           TF+ +GGF +E G +A+A+   DLVAY R+FL+NPDL +RF++DAPLN Y+ +TFYT DP
Sbjct: 306 TFISAGGFTREDGNEAVAKGRTDLVAYGRWFLANPDLPKRFQLDAPLNKYNRSTFYTSDP 365

Query: 125 VVGYSDYPSLDENS 139
           VVGY+DYPSL+  +
Sbjct: 366 VVGYTDYPSLESTA 374
[Close]
BLAST of Spo04307.1 vs. TAIR (Arabidopsis)
Match: AT1G76680.2 (12-oxophytodienoate reductase 1)

HSP 1 Score: 120.6 bits (301), Expect = 8.000e-28
Identity = 62/134 (46.27%), Postives = 90/134 (67.16%), Query Frame = 1 

		  

Query: 5   DSHDSDPLSLGLAVVERLNKRQEEIGLELPYLQIQAGTLTHGIGETEVELLRELRRAYQG 64
           +S D++P +LGL + E LNK     G+   ++ I+A   T G        L  +R+A++G
Sbjct: 269 ESGDTNPGALGLYMAESLNK----YGILYCHV-IEARMKTMGEVHACPHTLMPMRKAFKG 328

Query: 65  TFMISGGFNKELGMKAIAEDDADLVAYARFFLSNPDLVRRFEIDAPLNDYDAATFYTHDP 124
           TF+ +GGF +E G +A+++   DLVAY R+FL+NPDL +RF++DAPLN YD  TFYT DP
Sbjct: 329 TFISAGGFTREDGNEAVSKGRTDLVAYGRWFLANPDLPKRFQVDAPLNKYDRPTFYTSDP 388

Query: 125 VVGYSDYPSLDENS 139
           VVGY+DYP L+  +
Sbjct: 389 VVGYTDYPFLESTA 397
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BLAST of Spo04307.1 vs. TAIR (Arabidopsis)
Match: AT1G09400.1 (FMN-linked oxidoreductases superfamily protein)

HSP 1 Score: 84.3 bits (207), Expect = 6.300e-17
Identity = 47/108 (43.52%), Postives = 70/108 (64.81%), Query Frame = 1 

		  

Query: 5   DSHDSDPLSLGLAVVERLNKRQEEIGLELPYLQIQAGTLTHGIGETEVELLRELRRAYQG 64
           +S D+DP  LGL + + LN+ +    L    ++ +  T++  I E   E L  +R A+ G
Sbjct: 221 ESGDTDPKRLGLYMAKSLNRFEI---LYCHMIEPRMKTVSE-IFECR-ESLTPMRNAFNG 280

Query: 65  TFMISGGFNKELGMKAIAEDDADLVAYARFFLSNPDLVRRFEIDAPLN 113
           TF+++GG+ +E G KA+AE   DLVAY R FL+NPDL +RFE++APLN
Sbjct: 281 TFIVAGGYTREDGNKAVAEGRTDLVAYGRLFLANPDLPKRFELNAPLN 323
The following BLAST results are available for this feature:
BLAST of Spo04307.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902140180|gb|KNA04620.1|3.1e-74100.hypothetical protein SOVF_1978... [more]
gi|731338486|ref|XP_010680356.1|1.3e-5982.9PREDICTED: artemisinic aldehyd... [more]
gi|731338484|ref|XP_010680354.1|2.4e-5881.4PREDICTED: artemisinic aldehyd... [more]
gi|902140181|gb|KNA04621.1|2.5e-5577.7hypothetical protein SOVF_1978... [more]
gi|702304286|ref|XP_010049528.1|1.5e-3961.3PREDICTED: 12-oxophytodienoate... [more]
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BLAST of Spo04307.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9QBJ7_SPIOL2.2e-74100.Uncharacterized protein OS=Spi... [more]
A0A0J8C6V0_BETVU8.8e-6082.9Uncharacterized protein OS=Bet... [more]
A0A0J8CBA4_BETVU1.7e-5881.4Uncharacterized protein OS=Bet... [more]
A0A0K9QDM9_SPIOL1.7e-5577.7Uncharacterized protein OS=Spi... [more]
A0A059CVA1_EUCGR1.7e-3959.1Uncharacterized protein OS=Euc... [more]
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BLAST of Spo04307.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5
Match NameE-valueIdentityDescription
DBR2_ARTAN1.6e-3855.1Artemisinic aldehyde Delta(11(... [more]
OPR3_SOLLC1.4e-3756.912-oxophytodienoate reductase ... [more]
OPR3_ARATH8.8e-3756.812-oxophytodienoate reductase ... [more]
OPR7_ORYSJ3.7e-3553.112-oxophytodienoate reductase ... [more]
OPR6_ORYSJ4.0e-2948.9Putative 12-oxophytodienoate r... [more]
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BLAST of Spo04307.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 4
Match NameE-valueIdentityDescription
AT2G06050.25.0e-3856.8oxophytodienoate-reductase 3[more]
AT1G76690.16.1e-2847.012-oxophytodienoate reductase ... [more]
AT1G76680.28.0e-2846.212-oxophytodienoate reductase ... [more]
AT1G09400.16.3e-1743.5FMN-linked oxidoreductases sup... [more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001155NADH:flavin oxidoreductase/NADH oxidase, N-terminalPFAMPF00724Oxidored_FMNcoord: 50..112
score: 2.
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70coord: 3..135
score: 1.6
NoneNo IPR availablePANTHERPTHR22893NADH OXIDOREDUCTASE-RELATEDcoord: 4..140
score: 3.0
NoneNo IPR availablePANTHERPTHR22893:SF6712-OXOPHYTODIENOATE REDUCTASE 3coord: 4..140
score: 3.0
NoneNo IPR availableunknownSSF51395FMN-linked oxidoreductasescoord: 4..135
score: 1.33

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0009639 response to red or far red light
biological_process GO:0015827 tryptophan transport
biological_process GO:0055085 transmembrane transport
biological_process GO:0015804 neutral amino acid transport
biological_process GO:0015810 aspartate transmembrane transport
biological_process GO:0050789 regulation of biological process
biological_process GO:0009791 post-embryonic development
biological_process GO:0008152 metabolic process
biological_process GO:0009987 cellular process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0005515 protein binding
molecular_function GO:0016740 transferase activity
molecular_function GO:0010181 FMN binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0015172 acidic amino acid transmembrane transporter activity
molecular_function GO:0015175 neutral amino acid transmembrane transporter activity
molecular_function GO:0015293 symporter activity
molecular_function GO:0003824 catalytic activity
RNA-Seq Expression