Spo05472 (gene) | SpinachBase

Overview


Name	Spo05472
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein
Location	SpoScf_01737 : 83638 .. 83934 (-)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGCTGCAGAGAGGAAGAAGTGGCTATTTGCACTATTCTCCGCTTTGTTAGTCTCTCTCCTCCTCTTCCTCACAGCTATCTCAGGACTCAACGCCTCATATGCTTTCACTTCCTGGAGCAACAGGCCCACAGCACTCCCGAACCGTGGACCGCAGTTTCCACCGTCCTTCGCTTGCTACCTTTACGGCGGACCGGGAGATAAGGAGCAAATTTTCCGGCTATTGCTGGCCGTCTACCACCCACGGAATTGGTATTTGTTGCACCTCTCAGCCGAGGCATCGTTGTTGCGCTGA

mRNA sequence

ATGGGAGCTGCAGAGAGGAAGAAGTGGCTATTTGCACTATTCTCCGCTTTGTTAGTCTCTCTCCTCCTCTTCCTCACAGCTATCTCAGGACTCAACGCCTCATATGCTTTCACTTCCTGGAGCAACAGGCCCACAGCACTCCCGAACCGTGGACCGCAGTTTCCACCGTCCTTCGCTTGCTACCTTTACGGCGGACCGGGAGATAAGGAGCAAATTTTCCGGCTATTGCTGGCCGTCTACCACCCACGGAATTGGTATTTGTTGCACCTCTCAGCCGAGGCATCGTTGTTGCGCTGA

Coding sequence (CDS)

ATGGGAGCTGCAGAGAGGAAGAAGTGGCTATTTGCACTATTCTCCGCTTTGTTAGTCTCTCTCCTCCTCTTCCTCACAGCTATCTCAGGACTCAACGCCTCATATGCTTTCACTTCCTGGAGCAACAGGCCCACAGCACTCCCGAACCGTGGACCGCAGTTTCCACCGTCCTTCGCTTGCTACCTTTACGGCGGACCGGGAGATAAGGAGCAAATTTTCCGGCTATTGCTGGCCGTCTACCACCCACGGAATTGGTATTTGTTGCACCTCTCAGCCGAGGCATCGTTGTTGCGCTGA

Protein sequence

MGAAERKKWLFALFSALLVSLLLFLTAISGLNASYAFTSWSNRPTALPNRGPQFPPSFACYLYGGPGDKEQIFRLLLAVYHPRNWYLLHLSAEASLLR

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo05472.1	Spo05472.1	mRNA

Homology

[Close]

BLAST of Spo05472.1 vs. NCBI nr
Match: gi|902210884|gb|KNA16561.1| (hypothetical protein SOVF_088060 [Spinacia oleracea])

HSP 1 Score: 192.2 bits (487), Expect = 4.300e-46
Identity = 94/95 (98.95%), Postives = 94/95 (98.95%), Query Frame = 1


		  
Query: 1  MGAAERKKWLFALFSALLVSLLLFLTAISGLNASYAFTSWSNRPTALPNRGPQFPPSFAC 60
          MGAAERKKWLFALFSALLVSLLLFLTAISGLNASYAFTSWSNRPTALPNRGPQFPPSFA 
Sbjct: 1  MGAAERKKWLFALFSALLVSLLLFLTAISGLNASYAFTSWSNRPTALPNRGPQFPPSFAY 60

Query: 61 YLYGGPGDKEQIFRLLLAVYHPRNWYLLHLSAEAS 96
          YLYGGPGDKEQIFRLLLAVYHPRNWYLLHLSAEAS
Sbjct: 61 YLYGGPGDKEQIFRLLLAVYHPRNWYLLHLSAEAS 95

[Close]

BLAST of Spo05472.1 vs. NCBI nr
Match: gi|731340989|ref|XP_010681688.1| (PREDICTED: xylosyltransferase 1 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 163.3 bits (412), Expect = 2.100e-37
Identity = 80/98 (81.63%), Postives = 88/98 (89.80%), Query Frame = 1


		  
Query: 1  MGAAERKKWLFALFSALLVSLLLFLTAISGLNASYAFTS-WSNRPTALPNRGPQFPPSFA 60
          MGAAERKKWL ALFSALLVSLLLFLT+ISGLNASYAFTS W N+PT +P  GPQFPPSFA
Sbjct: 1  MGAAERKKWLLALFSALLVSLLLFLTSISGLNASYAFTSSWINKPTTIPTHGPQFPPSFA 60

Query: 61 CYLYGGPGDKEQIFRLLLAVYHPRNWYLLHLSAEASLL 98
           Y+YGG GDKE++ RLLLAVYHPRNWYLLHLS++AS L
Sbjct: 61 YYIYGGGGDKERMLRLLLAVYHPRNWYLLHLSSDASTL 98

[Close]

BLAST of Spo05472.1 vs. NCBI nr
Match: gi|976910924|gb|KVH97588.1| (Glycosyl transferase, family 14, partial [Cynara cardunculus var. scolymus])

HSP 1 Score: 104.4 bits (259), Expect = 1.200e-19
Identity = 55/95 (57.89%), Postives = 70/95 (73.68%), Query Frame = 1


		  
Query: 1  MGAAERKKWLFALFSALLVSLLLFLTAISGLNASYAFTSWSNRPTALPNRGPQFPPSFAC 60
          MGA   KKWLF LF A  +SLLLFL++I G ++SYAFTS  ++P +  +RG  +PP+FA 
Sbjct: 1  MGA--EKKWLFTLFFAAFISLLLFLSSICGFSSSYAFTS--HKPFSAVHRGRGYPPAFAY 60

Query: 61 YLYGGPGDKEQIFRLLLAVYHPRNWYLLHLSAEAS 96
          Y+ G  GD ++IFRLLLAVYHPRN YLLH+  E S
Sbjct: 61 YITGTRGDADRIFRLLLAVYHPRNRYLLHIGTEGS 91

[Close]

BLAST of Spo05472.1 vs. NCBI nr
Match: gi|225424176|ref|XP_002284096.1| (PREDICTED: xylosyltransferase 1 [Vitis vinifera])

HSP 1 Score: 104.0 bits (258), Expect = 1.500e-19
Identity = 55/89 (61.80%), Postives = 67/89 (75.28%), Query Frame = 1


		  
Query: 7  KKWLFALFSALLVSLLLFLTAISGLNASYAFTSWSNRPTALPNRGPQFPPSFACYLYGGP 66
          KKWLF LFSA  VSLLLFL++ISG NASYA  S+    ++  + G  +PP+FA Y+ GG 
Sbjct: 5  KKWLFTLFSAAFVSLLLFLSSISGFNASYAL-SFHKPFSSTVHHGLHYPPAFAYYISGGR 64

Query: 67 GDKEQIFRLLLAVYHPRNWYLLHLSAEAS 96
          G K++I RLLLAVYHPRN YLLHL A+AS
Sbjct: 65 GHKDRILRLLLAVYHPRNRYLLHLGADAS 92

[Close]

BLAST of Spo05472.1 vs. NCBI nr
Match: gi|661896166|emb|CDP00744.1| (unnamed protein product [Coffea canephora])

HSP 1 Score: 102.4 bits (254), Expect = 4.500e-19
Identity = 51/95 (53.68%), Postives = 65/95 (68.42%), Query Frame = 1


		  
Query: 1  MGAAERKKWLFALFSALLVSLLLFLTAISGLNASYAFTSWSNRPTALPNRGPQFPPSFAC 60
          MG    KKWLFALFSA  +S L+F+++I GL++S+   S     T   +RGP  PP+FA 
Sbjct: 1  MGVGAEKKWLFALFSAAFLSFLIFISSICGLSSSFYSYSSQKPYTLSVDRGPGHPPAFAY 60

Query: 61 YLYGGPGDKEQIFRLLLAVYHPRNWYLLHLSAEAS 96
          Y+ GG GD  +IFRL LAVYHPRN YLLH+ A+ S
Sbjct: 61 YISGGRGDANRIFRLFLAVYHPRNRYLLHIGADGS 95

[Close]

BLAST of Spo05472.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RAN9_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_088060 PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 3.000e-46
Identity = 94/95 (98.95%), Postives = 94/95 (98.95%), Query Frame = 1


		  
Query: 1  MGAAERKKWLFALFSALLVSLLLFLTAISGLNASYAFTSWSNRPTALPNRGPQFPPSFAC 60
          MGAAERKKWLFALFSALLVSLLLFLTAISGLNASYAFTSWSNRPTALPNRGPQFPPSFA 
Sbjct: 1  MGAAERKKWLFALFSALLVSLLLFLTAISGLNASYAFTSWSNRPTALPNRGPQFPPSFAY 60

Query: 61 YLYGGPGDKEQIFRLLLAVYHPRNWYLLHLSAEAS 96
          YLYGGPGDKEQIFRLLLAVYHPRNWYLLHLSAEAS
Sbjct: 61 YLYGGPGDKEQIFRLLLAVYHPRNWYLLHLSAEAS 95

[Close]

BLAST of Spo05472.1 vs. UniProtKB/TrEMBL
Match: A0A0J8C311_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_6g142830 PE=4 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 1.500e-37
Identity = 80/98 (81.63%), Postives = 88/98 (89.80%), Query Frame = 1


		  
Query: 1  MGAAERKKWLFALFSALLVSLLLFLTAISGLNASYAFTS-WSNRPTALPNRGPQFPPSFA 60
          MGAAERKKWL ALFSALLVSLLLFLT+ISGLNASYAFTS W N+PT +P  GPQFPPSFA
Sbjct: 1  MGAAERKKWLLALFSALLVSLLLFLTSISGLNASYAFTSSWINKPTTIPTHGPQFPPSFA 60

Query: 61 CYLYGGPGDKEQIFRLLLAVYHPRNWYLLHLSAEASLL 98
           Y+YGG GDKE++ RLLLAVYHPRNWYLLHLS++AS L
Sbjct: 61 YYIYGGGGDKERMLRLLLAVYHPRNWYLLHLSSDASTL 98

[Close]

BLAST of Spo05472.1 vs. UniProtKB/TrEMBL
Match: A0A103XVE4_CYNCS (Glycosyl transferase, family 14 (Fragment) OS=Cynara cardunculus var. scolymus GN=Ccrd_000254 PE=4 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 8.200e-20
Identity = 55/95 (57.89%), Postives = 70/95 (73.68%), Query Frame = 1


		  
Query: 1  MGAAERKKWLFALFSALLVSLLLFLTAISGLNASYAFTSWSNRPTALPNRGPQFPPSFAC 60
          MGA   KKWLF LF A  +SLLLFL++I G ++SYAFTS  ++P +  +RG  +PP+FA 
Sbjct: 1  MGA--EKKWLFTLFFAAFISLLLFLSSICGFSSSYAFTS--HKPFSAVHRGRGYPPAFAY 60

Query: 61 YLYGGPGDKEQIFRLLLAVYHPRNWYLLHLSAEAS 96
          Y+ G  GD ++IFRLLLAVYHPRN YLLH+  E S
Sbjct: 61 YITGTRGDADRIFRLLLAVYHPRNRYLLHIGTEGS 91

[Close]

BLAST of Spo05472.1 vs. UniProtKB/TrEMBL
Match: D7T8V4_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0011g04680 PE=4 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 1.100e-19
Identity = 55/89 (61.80%), Postives = 67/89 (75.28%), Query Frame = 1


		  
Query: 7  KKWLFALFSALLVSLLLFLTAISGLNASYAFTSWSNRPTALPNRGPQFPPSFACYLYGGP 66
          KKWLF LFSA  VSLLLFL++ISG NASYA  S+    ++  + G  +PP+FA Y+ GG 
Sbjct: 5  KKWLFTLFSAAFVSLLLFLSSISGFNASYAL-SFHKPFSSTVHHGLHYPPAFAYYISGGR 64

Query: 67 GDKEQIFRLLLAVYHPRNWYLLHLSAEAS 96
          G K++I RLLLAVYHPRN YLLHL A+AS
Sbjct: 65 GHKDRILRLLLAVYHPRNRYLLHLGADAS 92

[Close]

BLAST of Spo05472.1 vs. UniProtKB/TrEMBL
Match: A0A068TXW5_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00032813001 PE=4 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 3.100e-19
Identity = 51/95 (53.68%), Postives = 65/95 (68.42%), Query Frame = 1


		  
Query: 1  MGAAERKKWLFALFSALLVSLLLFLTAISGLNASYAFTSWSNRPTALPNRGPQFPPSFAC 60
          MG    KKWLFALFSA  +S L+F+++I GL++S+   S     T   +RGP  PP+FA 
Sbjct: 1  MGVGAEKKWLFALFSAAFLSFLIFISSICGLSSSFYSYSSQKPYTLSVDRGPGHPPAFAY 60

Query: 61 YLYGGPGDKEQIFRLLLAVYHPRNWYLLHLSAEAS 96
          Y+ GG GD  +IFRL LAVYHPRN YLLH+ A+ S
Sbjct: 61 YISGGRGDANRIFRLFLAVYHPRNRYLLHIGADGS 95

[Close]

BLAST of Spo05472.1 vs. TAIR (Arabidopsis)
Match: AT1G71070.1 (Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein)

HSP 1 Score: 82.4 bits (202), Expect = 1.700e-16
Identity = 46/96 (47.92%), Postives = 63/96 (65.62%), Query Frame = 1


		  
Query: 1  MGAAERKKWLFALFSALLVSLLLFLTAISGLNASYAFTSWSNRPTALPNR-GPQFPPSFA 60
          MGA   KKWLF LFS + +S+ L L         Y+ ++++++P     R G  +PP+FA
Sbjct: 1  MGA--EKKWLFTLFSVVFLSVFLLLL--------YSISAFTSKPFPSSIRHGAHYPPAFA 60

Query: 61 CYLYGGPGDKEQIFRLLLAVYHPRNWYLLHLSAEAS 96
           Y+ GG GD ++I RLLLAVYHPRN YL+HL AEA+
Sbjct: 61 YYITGGRGDNDRISRLLLAVYHPRNRYLIHLGAEAT 86

[Close]

BLAST of Spo05472.1 vs. TAIR (Arabidopsis)
Match: AT1G03520.1 (Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein)

HSP 1 Score: 52.0 bits (123), Expect = 2.400e-7
Identity = 33/107 (30.84%), Postives = 50/107 (46.73%), Query Frame = 1


		  
Query: 7   KKWLFALFSALLVSLLLFLTAISGL-------------------NASYAFTSWSNRPTAL 66
           +KWLF   ++L++S+ L +  ISG                    N  +  + +     + 
Sbjct: 34  RKWLFPFLASLIMSITLLILLISGQFDNFFGEEDQLPVDVVSESNDYFVESDFKQSMNST 93

Query: 67  PNRGPQFPPSFACYLYGGPGDKEQIFRLLLAVYHPRNWYLLHLSAEA 95
            +  P+ PP  A  + G  GD  ++ R L AVYHPRN Y+LHL  EA
Sbjct: 94  ADVNPE-PPRLAYLISGTKGDSHRMMRTLQAVYHPRNQYVLHLDLEA 139

[Close]

BLAST of Spo05472.1 vs. TAIR (Arabidopsis)
Match: AT3G24040.1 (Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein)

HSP 1 Score: 51.2 bits (121), Expect = 4.200e-7
Identity = 35/97 (36.08%), Postives = 50/97 (51.55%), Query Frame = 1


		  
Query: 10  LFALFSALLVSLLLFLTAISGLNASYAFTSWSNRPTAL---PNRGPQF--------PPSF 69
           L+ + +   +SL   L+  S  ++ Y+ +S++ RP  L   P   P          PPS 
Sbjct: 12  LYIILTTAFLSLCFLLSLSS--SSQYSSSSYTGRPDDLRPDPRLFPSSSKIAADTAPPSI 71

Query: 70  ACYLYGGPGDKEQIFRLLLAVYHPRNWYLLHLSAEAS 96
           A  + G  GD  +I RLL A YHPRN YLLHL + A+
Sbjct: 72  AYLISGSSGDTRRILRLLYATYHPRNRYLLHLDSLAT 106

[Close]

BLAST of Spo05472.1 vs. TAIR (Arabidopsis)
Match: AT2G37585.1 (Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein)

HSP 1 Score: 49.7 bits (117), Expect = 1.200e-6
Identity = 22/40 (55.00%), Postives = 29/40 (72.50%), Query Frame = 1


		  
Query: 56 PSFACYLYGGPGDKEQIFRLLLAVYHPRNWYLLHLSAEAS 96
          P FA  + G  GD +++ RLL A++HPRN+YLLHL  EAS
Sbjct: 57 PRFAYLVTGTKGDGKRVKRLLKAIHHPRNYYLLHLDLEAS 96

[Close]

BLAST of Spo05472.1 vs. TAIR (Arabidopsis)
Match: AT5G39990.1 (Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein)

HSP 1 Score: 48.5 bits (114), Expect = 2.700e-6
Identity = 36/111 (32.43%), Postives = 52/111 (46.85%), Query Frame = 1


		  
Query: 4   AERKKWLF--ALFSALLVSLLLFLTA-ISGLNASYAFTSWSNRPTALPNRG--------- 63
           +  +KW+F   L  ++    LLFLT  ++       F  ++ RP  L   G         
Sbjct: 29  SSERKWIFFPLLIGSIFALFLLFLTTTLTSPTGGVRFLPFT-RPVLLTGSGSSAFVESKI 88

Query: 64  -PQ------FPPSFACYLYGGPGDKEQIFRLLLAVYHPRNWYLLHLSAEAS 96
            PQ       PP FA  + G  GD + + R LLA+YHP N Y++HL  E+S
Sbjct: 89  KPQQISSLPSPPRFAYLISGSAGDGKSLRRTLLALYHPNNRYVVHLDRESS 138

The following BLAST results are available for this feature:

BLAST of Spo05472.1 vs. NCBI nr
BLAST of Spo05472.1 vs. UniProtKB/TrEMBL
BLAST of Spo05472.1 vs. ExPASy Swiss-Prot
BLAST of Spo05472.1 vs. TAIR (Arabidopsis)

BLAST of Spo05472.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902210884\|gb\|KNA16561.1\|	4.3e-46	98.9	hypothetical protein SOVF_0880...	[more]
gi\|731340989\|ref\|XP_010681688.1\|	2.1e-37	81.6	PREDICTED: xylosyltransferase ...	[more]
gi\|976910924\|gb\|KVH97588.1\|	1.2e-19	57.8	Glycosyl transferase, family 1...	[more]
gi\|225424176\|ref\|XP_002284096.1\|	1.5e-19	61.8	PREDICTED: xylosyltransferase ...	[more]
gi\|661896166\|emb\|CDP00744.1\|	4.5e-19	53.6	unnamed protein product [Coffe...	[more]

BLAST of Spo05472.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RAN9_SPIOL	3.0e-46	98.9	Uncharacterized protein OS=Spi...	[more]
A0A0J8C311_BETVU	1.5e-37	81.6	Uncharacterized protein OS=Bet...	[more]
A0A103XVE4_CYNCS	8.2e-20	57.8	Glycosyl transferase, family 1...	[more]
D7T8V4_VITVI	1.1e-19	61.8	Putative uncharacterized prote...	[more]
A0A068TXW5_COFCA	3.1e-19	53.6	Uncharacterized protein OS=Cof...	[more]

BLAST of Spo05472.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Spo05472.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT1G71070.1	1.7e-16	47.9	Core-2/I-branching beta-1,6-N-...	[more]
AT1G03520.1	2.4e-7	30.8	Core-2/I-branching beta-1,6-N-...	[more]
AT3G24040.1	4.2e-7	36.0	Core-2/I-branching beta-1,6-N-...	[more]
AT2G37585.1	1.2e-6	55.0	Core-2/I-branching beta-1,6-N-...	[more]
AT5G39990.1	2.7e-6	32.4	Core-2/I-branching beta-1,6-N-...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR19297	GLYCOSYLTRANSFERASE 14 FAMILY MEMBER	coord: 6..95 score: 2.1
None	No IPR available	PANTHER	PTHR19297:SF70	SUBFAMILY NOT NAMED	coord: 6..95 score: 2.1

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
biological_process	GO:0000186	activation of MAPKK activity
biological_process	GO:0009069	serine family amino acid metabolic process
biological_process	GO:0007178	transmembrane receptor protein serine/threonine kinase signaling pathway
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0016020	membrane
cellular_component	GO:0005667	transcription factor complex
molecular_function	GO:0008375	acetylglucosaminyltransferase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004709	MAP kinase kinase kinase activity
molecular_function	GO:0005515	protein binding
molecular_function	GO:0003712	transcription coregulator activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo21077	0.73	Barchart \| Table
Spo12918	0.73	Barchart \| Table
Spo25507	0.73	Barchart \| Table
Spo04409	0.71	Barchart \| Table
Spo09855	0.70	Barchart \| Table
Spo02469	0.69	Barchart \| Table
Spo01128	0.68	Barchart \| Table
Spo02609	0.68	Barchart \| Table
Spo06497	0.68	Barchart \| Table
Spo09711	0.67	Barchart \| Table
Spo19394	0.67	Barchart \| Table
Spo04114	0.67	Barchart \| Table
Spo26272	0.67	Barchart \| Table
Spo16378	0.66	Barchart \| Table
Spo06873	0.66	Barchart \| Table
Spo07916	0.66	Barchart \| Table
Spo14267	0.66	Barchart \| Table
Spo15994	0.66	Barchart \| Table
Spo18221	0.65	Barchart \| Table