Spo06250 (gene)

Overview
NameSpo06250
Typegene
OrganismSpinacia oleracea (Spinach)
DescriptionCytokinin oxidase/dehydrogenase-like
LocationSpoScf_00766 : 52730 .. 56610 (-)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGTCGTGTAAGTTCTTTAGACAAAGTGCAGAGATGCTGCTAAAAGTTTTCTTAGTTCTTTTGTTAAACTTGGCTGAGCATAATATCAATCATTGCTCTTACCTTCCTCATGCCACCCCAGATTTCACAAACTTAAGCTCACTTGAGCTAGTATCATCACTTAAGAAACTAAATCTAAATGGTAATTTCGATTTCGAAAATACCCGCCGTGTGGCAAATGACTTTGGTAACAGATACCATTTTCTTCCGATGGCGGTTCTGTTACCCAAAACAGTCTCTGACATCTCTACAACCATCAAACATGTTATCAACATGGGTCATAACTCAGAGCTCACTGTTACAGCAAGAGGCCATGGTCACTCACTTCAAGGTCAATCTCAAAGCCATGGTGGCATTGTCATCAGCATGGAATCTCTAGAAGAACCAAAAATGCATGTCCATACAGGCGAGCAACCTTATGTTGATGTTTCGGGGGGTGAATTGTGGATTAACATTCTGCATGAGACCCTAAAACAGGGATTATCACCTAAATCATGGACCGACTACCTTTATTTGACTGTCGGGGGAACTTTATCAAATGCCGGGATTAGTGGACAAGCATTCAGATATGGACCTCAAATAAACAATGTCCATCGGCTGGAAATTGTAACTGGTAAGTTTTTAGCTCTGTTCTATTTAAATTCTTGAATATCAGATGAATAAATTCGGGCATCTTATATTTCAGCATATAAGTGTCATTAAACAAGATCACATTTAATGCAACATGTCGGCTATTTTGTTAATTTCATGGGTGCAGGAAAAGGAGATATATTGAACTGTTCGGCTAAAGAGAACGCTGACCTCTTTTATGGTGTTCTTGGGGGACTAGGGCAGTTTGGCATCATCACTAGGGCCAGAATCTCTCTAGAGCCAGCTCCCAGATCGGTAAGTATCCCACCGTTAATCTGACTTCAACTGTTGAATCATAAAGGTCCTTGTGGTCATCTGAAAACTTTTTCCACACTCCGACAGGTGAAATGGATTAGAGTACTATACTCAGATTTCAACTTATTTACCAAGGATCAAGAACAGCTAATATCATCAGATAATTCATTTGATTATATTGAAGGCTTCGTCATAATAAACAGGACAGGTCTAATTAATAACTGGAGATCTACCTTCAGCCCCAGAGAACCCCTGCAGGCTAGTCAATTCAGCTCTGAAGGAAAAACTCTCTACTGCCTCGAAATGGCCAAGTACTTTAATCCTGAGGATGGGAACATTATAAACCAGGTTAGAATCTCTAACTACCTTCTCATATGCCGGTAAACGTAAAAATCATGATTCAGGAATGAACCTCTTGTGGCTTTCAAATTGCAGGAAACAGAACATTTGCTGTCCAAATTAAACTATATCTCCCACACACTTTTCCAGTCAGAAGTATCGTATGTAAATTTCCTAAACAGAGTTCACCTATCAGAAATAAAGCTCCGAGAGAAAGGTTTGTGGGATGTTCCTCATCCTTGGTTAAATCTTCTGGTTCCCAAGAGTAAGATATACAGTTTTGCTGAGGAGGTATTCGGTCATATCCTCACAGATACAAGCAACGGCCCAATCCTCATTTACCCCGTAAACCAGTCAAGGTACTTGCTTAACTGAATGTGCTTGTCTATGTCTACTATTCCACCTCATAGAGAAGACTAAAGTGGAATCAATTTTTTTTTGGGAATGTGTGTATTATGCTTAACAGATGGAATCACAAGACGTCGATGGTGACCCCTTCTGAAGATGTATTTTACCTTGTAGCATTTCTAACATCTGCAATGCCATCTTCGTCTGGATCAGACGGGCTCGAAAATATTTTAACCAAAAACAAAAGAATATTAGACTTCTGTGAGATGGCCAAGCTTGGGGCAAAGCAATATCTGCCACATTTTGACACTCAGGATGGATGGAGAGCTCATTTTGGGCCTCGATGGGATGTTTTTGCAAGAAGAAAACTAACCTATGACCCTCTATCAATTCTCACACCAGGGCAAAGAATCTTCCGCAAAGGAAGAGCCATTTCATGATATTGGCTGACATCTCAAGAACCTCATTCAAATTGGTCACTCCAAAAGTCAAAACAAAAAAGAGAGAAGCATCAAAGCCTAAGTCTGTAAGTAGACCATCTACATAGCTAGCACATCTTTCAGCATGTTAGTATCTCTACAAGTTCACAGAGTACAGCATTCATTGATTTAAATTCTTGTTCTACACGTTTTCATAACAATTTGGAACAAGACACCCATTTTTGTATCCGAGTTTAAAGATGAGTTGATACAGTTGCATATGCCAATGAGAAAGTAAAGTTAGAAAGACAACCACAGAACAGAGAAATATAGAATGTTTCAAAGGTTGATAGAGATTTCGGAAAAGGATTCTTAAATTGACCCACAGGAAAAGACACAGCAAGAGTGTCTTTTGGTAGTCAAAGCAAGTCAACAACAAAGCACAGAACCATAAAGAACTAGAAACAGTAGCTGAATGTATTACAAACGTACCTTTAGCATTTTAAAGTTTGTGACTATAGTTGTGAGTTGCGACTAGACTAAAATCTGAAAGAAATTTCCTGAAACAACAGTTTTAGTCTGACACTCTATCTTCACTCCAGCCAAGGCCTGGTCGGCGTTTCTCACCCTGGGGTTCTTCATAGTCACGTGCATCAAGTAAAGAAGCTTCAAAGTCAGTTGAGTCGCTTGCGATAGGAAGAGGACTACTTAAATCATCTCTGGCACCAAAACCTCCAGCTCGTACAACATCATCAGGATTTATAGCTGCCTCCATATTTATATCTGTTGATGTGTCACAATGTCCAGGTGCTTCCTTTAGTGCAATTTCTCCCTCAGATTGGGGATCATTCAATCTGAATTGTACAGTACCTTCAGCAGAGAATGAATCCTGCTTAGGCATAACATCCCCTTGAGCCCTCTTAGATTCAGCTCCTCCTTTGCTTTGGACACTGTCAATCTTATCTGACATGTATAAAGCTCCTTTAAGTATAAGTGAAGGAAGGAACAAAAGTACGAGATACAACAACGTTCAAGAAATAAGGAAACTAAGAATCGTATGTAACCAACAAGTAGTGAAGACGACCGTAGTTTAGAACATCATGCCAAAACAACCACAGGACATATGCAAACACAAGAAGATACGGAGATTGAAAACTAACAACTAAATACAACCATAAACCACTCAATCGAAGAACATGGTGATTAAGGAATATTTAAATGGTGATAATGCAAATCTCAAGAGTCAAGACAACGAATTCCAGAAATGGGACACTTTTCAGCTAGATGCGGCTTAAAATTTTCCTGTTCCAGATTTTAACAAATGACATAATTATCTGATACCAAACACCGTCAATAAAAAGCTAACATCAGCAGGATGCCTCATGGTTAAATTGACACAGTAAAGTGCATTGAGAGGAAGTAATGACCTGGTCTATACTAATTTAGAACTAATAATATTCTTGACAGAAAATCATACATAAGCACCAAAGAGTTTTAGAAGAGTAGATCTAAAGATCAAGTTGTAAACAAAATTGAAGTAGCAAGGGCCAGTACCAATTTTTTTTCCAGTTCCTTTATCGTCCATGATGGGTTTGAATATACACAAGTGGCACCGTCAGCAAGCTGTTTTTAAGTCACGTAAACAGATTAATACATCCTGCAAAAATACAAATCTCAAATAATTGTCCAAACTCTATGATGGAAGACAGTTTTAAGGGCCAACCAATGTAAATCTTACATTCAACTATAGTAGGAGTTTCAAATCAGCAGATGTAGTTGTTCTGAATCTAAAACTAGAAGGATAGAGTCGATAGACTGAGAGAATATATAACTTATTCTCAACCATAT

mRNA sequence

ATGTTGTCGTGTAAGTTCTTTAGACAAAGTGCAGAGATGCTGCTAAAAGTTTTCTTAGTTCTTTTGTTAAACTTGGCTGAGCATAATATCAATCATTGCTCTTACCTTCCTCATGCCACCCCAGATTTCACAAACTTAAGCTCACTTGAGCTAGTATCATCACTTAAGAAACTAAATCTAAATGGTAATTTCGATTTCGAAAATACCCGCCGTGTGGCAAATGACTTTGGTAACAGATACCATTTTCTTCCGATGGCGGTTCTGTTACCCAAAACAGTCTCTGACATCTCTACAACCATCAAACATGTTATCAACATGGGTCATAACTCAGAGCTCACTGTTACAGCAAGAGGCCATGGTCACTCACTTCAAGGTCAATCTCAAAGCCATGGTGGCATTGTCATCAGCATGGAATCTCTAGAAGAACCAAAAATGCATGTCCATACAGGCGAGCAACCTTATGTTGATGTTTCGGGGGGTGAATTGTGGATTAACATTCTGCATGAGACCCTAAAACAGGGATTATCACCTAAATCATGGACCGACTACCTTTATTTGACTGTCGGGGGAACTTTATCAAATGCCGGGATTAGTGGACAAGCATTCAGATATGGACCTCAAATAAACAATGTCCATCGGCTGGAAATTGTAACTGGAAAAGGAGATATATTGAACTGTTCGGCTAAAGAGAACGCTGACCTCTTTTATGGTGTTCTTGGGGGACTAGGGCAGTTTGGCATCATCACTAGGGCCAGAATCTCTCTAGAGCCAGCTCCCAGATCGGTGAAATGGATTAGAGTACTATACTCAGATTTCAACTTATTTACCAAGGATCAAGAACAGCTAATATCATCAGATAATTCATTTGATTATATTGAAGGCTTCGTCATAATAAACAGGACAGGTCTAATTAATAACTGGAGATCTACCTTCAGCCCCAGAGAACCCCTGCAGGCTAGTCAATTCAGCTCTGAAGGAAAAACTCTCTACTGCCTCGAAATGGCCAAGTACTTTAATCCTGAGGATGGGAACATTATAAACCAGGAAACAGAACATTTGCTGTCCAAATTAAACTATATCTCCCACACACTTTTCCAGTCAGAAGTATCGTATGTAAATTTCCTAAACAGAGTTCACCTATCAGAAATAAAGCTCCGAGAGAAAGGTTTGTGGGATGTTCCTCATCCTTGGTTAAATCTTCTGGTTCCCAAGAGTAAGATATACAGTTTTGCTGAGGAGGTATTCGGTCATATCCTCACAGATACAAGCAACGGCCCAATCCTCATTTACCCCGTAAACCAGTCAAGATGGAATCACAAGACGTCGATGGTGACCCCTTCTGAAGATGTATTTTACCTTGTAGCATTTCTAACATCTGCAATGCCATCTTCGTCTGGATCAGACGGGCTCGAAAATATTTTAACCAAAAACAAAAGAATATTAGACTTCTGTGAGATGGCCAAGCTTGGGGCAAAGCAATATCTGCCACATTTTGACACTCAGGATGGATGGAGAGCTCATTTTGGGCCTCGATGGGATGTTTTTGCAAGAAGAAAACTAACCTATGACCCTCTATCAATTCTCACACCAGGGCAAAGAATCTTCCGCAAAGGAAGAGCCATTTCATGATATTGGCTGACATCTCAAGAACCTCATTCAAATTGGTCACTCCAAAAGTCAAAACAAAAAAGAGAGAAGCATCAAAGCCTAAGTCTGTAAGTAGACCATCTACATAGCTAGCACATCTTTCAGCATGTTAGTATCTCTACAAGTTCACAGAGTACAGCATTCATTGATTTAAATTCTTGTTCTACACGTTTTCATAACAATTTGGAACAAGACACCCATTTTTGTATCCGAGTTTAAAGATGAGTTGATACAGTTGCATATGCCAATGAGAAAGTAAAGTTAGAAAGACAACCACAGAACAGAGAAATATAGAATGTTTCAAAGGTTGATAGAGATTTCGGAAAAGGATTCTTAAATTGACCCACAGGAAAAGACACAGCAAGAGTGTCTTTTGGTAGTCAAAGCAAGTCAACAACAAAGCACAGAACCATAAAGAACTAGAAACAGTAGCTGAATGTATTACAAACGTACCTTTAGCATTTTAAAGTTTGTGACTATAGTTGTGAGTTGCGACTAGACTAAAATCTGAAAGAAATTTCCTGAAACAACAGTTTTAGTCTGACACTCTATCTTCACTCCAGCCAAGGCCTGGTCGGCGTTTCTCACCCTGGGGTTCTTCATAGTCACGTGCATCAAGTAAAGAAGCTTCAAAGTCAGTTGAGTCGCTTGCGATAGGAAGAGGACTACTTAAATCATCTCTGGCACCAAAACCTCCAGCTCGTACAACATCATCAGGATTTATAGCTGCCTCCATATTTATATCTGTTGATGTGTCACAATGTCCAGGTGCTTCCTTTAGTGCAATTTCTCCCTCAGATTGGGGATCATTCAATCTGAATTGTACAGTACCTTCAGCAGAGAATGAATCCTGCTTAGGCATAACATCCCCTTGAGCCCTCTTAGATTCAGCTCCTCCTTTGCTTTGGACACTGTCAATCTTATCTGACATGTATAAAGCTCCTTTAAGTATAAGTGAAGGAAGGAACAAAAGTACGAGATACAACAACGTTCAAGAAATAAGGAAACTAAGAATCGTATGTAACCAACAAGTAGTGAAGACGACCGTAGTTTAGAACATCATGCCAAAACAACCACAGGACATATGCAAACACAAGAAGATACGGAGATTGAAAACTAACAACTAAATACAACCATAAACCACTCAATCGAAGAACATGGTGATTAAGGAATATTTAAATGGTGATAATGCAAATCTCAAGAGTCAAGACAACGAATTCCAGAAATGGGACACTTTTCAGCTAGATGCGGCTTAAAATTTTCCTGTTCCAGATTTTAACAAATGACATAATTATCTGATACCAAACACCGTCAATAAAAAGCTAACATCAGCAGGATGCCTCATGGTTAAATTGACACAGTAAAGTGCATTGAGAGGAAGTAATGACCTGGTCTATACTAATTTAGAACTAATAATATTCTTGACAGAAAATCATACATAAGCACCAAAGAGTTTTAGAAGAGTAGATCTAAAGATCAAGTTGTAAACAAAATTGAAGTAGCAAGGGCCAGTACCAATTTTTTTTCCAGTTCCTTTATCGTCCATGATGGGTTTGAATATACACAAGTGGCACCGTCAGCAAGCTGTTTTTAAGTCACGTAAACAGATTAATACATCCTGCAAAAATACAAATCTCAAATAATTGTCCAAACTCTATGATGGAAGACAGTTTTAAGGGCCAACCAATGTAAATCTTACATTCAACTATAGTAGGAGTTTCAAATCAGCAGATGTAGTTGTTCTGAATCTAAAACTAGAAGGATAGAGTCGATAGACTGAGAGAATATATAACTTATTCTCAACCATAT

Coding sequence (CDS)

ATGTTGTCGTGTAAGTTCTTTAGACAAAGTGCAGAGATGCTGCTAAAAGTTTTCTTAGTTCTTTTGTTAAACTTGGCTGAGCATAATATCAATCATTGCTCTTACCTTCCTCATGCCACCCCAGATTTCACAAACTTAAGCTCACTTGAGCTAGTATCATCACTTAAGAAACTAAATCTAAATGGTAATTTCGATTTCGAAAATACCCGCCGTGTGGCAAATGACTTTGGTAACAGATACCATTTTCTTCCGATGGCGGTTCTGTTACCCAAAACAGTCTCTGACATCTCTACAACCATCAAACATGTTATCAACATGGGTCATAACTCAGAGCTCACTGTTACAGCAAGAGGCCATGGTCACTCACTTCAAGGTCAATCTCAAAGCCATGGTGGCATTGTCATCAGCATGGAATCTCTAGAAGAACCAAAAATGCATGTCCATACAGGCGAGCAACCTTATGTTGATGTTTCGGGGGGTGAATTGTGGATTAACATTCTGCATGAGACCCTAAAACAGGGATTATCACCTAAATCATGGACCGACTACCTTTATTTGACTGTCGGGGGAACTTTATCAAATGCCGGGATTAGTGGACAAGCATTCAGATATGGACCTCAAATAAACAATGTCCATCGGCTGGAAATTGTAACTGGAAAAGGAGATATATTGAACTGTTCGGCTAAAGAGAACGCTGACCTCTTTTATGGTGTTCTTGGGGGACTAGGGCAGTTTGGCATCATCACTAGGGCCAGAATCTCTCTAGAGCCAGCTCCCAGATCGGTGAAATGGATTAGAGTACTATACTCAGATTTCAACTTATTTACCAAGGATCAAGAACAGCTAATATCATCAGATAATTCATTTGATTATATTGAAGGCTTCGTCATAATAAACAGGACAGGTCTAATTAATAACTGGAGATCTACCTTCAGCCCCAGAGAACCCCTGCAGGCTAGTCAATTCAGCTCTGAAGGAAAAACTCTCTACTGCCTCGAAATGGCCAAGTACTTTAATCCTGAGGATGGGAACATTATAAACCAGGAAACAGAACATTTGCTGTCCAAATTAAACTATATCTCCCACACACTTTTCCAGTCAGAAGTATCGTATGTAAATTTCCTAAACAGAGTTCACCTATCAGAAATAAAGCTCCGAGAGAAAGGTTTGTGGGATGTTCCTCATCCTTGGTTAAATCTTCTGGTTCCCAAGAGTAAGATATACAGTTTTGCTGAGGAGGTATTCGGTCATATCCTCACAGATACAAGCAACGGCCCAATCCTCATTTACCCCGTAAACCAGTCAAGATGGAATCACAAGACGTCGATGGTGACCCCTTCTGAAGATGTATTTTACCTTGTAGCATTTCTAACATCTGCAATGCCATCTTCGTCTGGATCAGACGGGCTCGAAAATATTTTAACCAAAAACAAAAGAATATTAGACTTCTGTGAGATGGCCAAGCTTGGGGCAAAGCAATATCTGCCACATTTTGACACTCAGGATGGATGGAGAGCTCATTTTGGGCCTCGATGGGATGTTTTTGCAAGAAGAAAACTAACCTATGACCCTCTATCAATTCTCACACCAGGGCAAAGAATCTTCCGCAAAGGAAGAGCCATTTCATGA

Protein sequence

MLSCKFFRQSAEMLLKVFLVLLLNLAEHNINHCSYLPHATPDFTNLSSLELVSSLKKLNLNGNFDFENTRRVANDFGNRYHFLPMAVLLPKTVSDISTTIKHVINMGHNSELTVTARGHGHSLQGQSQSHGGIVISMESLEEPKMHVHTGEQPYVDVSGGELWINILHETLKQGLSPKSWTDYLYLTVGGTLSNAGISGQAFRYGPQINNVHRLEIVTGKGDILNCSAKENADLFYGVLGGLGQFGIITRARISLEPAPRSVKWIRVLYSDFNLFTKDQEQLISSDNSFDYIEGFVIINRTGLINNWRSTFSPREPLQASQFSSEGKTLYCLEMAKYFNPEDGNIINQETEHLLSKLNYISHTLFQSEVSYVNFLNRVHLSEIKLREKGLWDVPHPWLNLLVPKSKIYSFAEEVFGHILTDTSNGPILIYPVNQSRWNHKTSMVTPSEDVFYLVAFLTSAMPSSSGSDGLENILTKNKRILDFCEMAKLGAKQYLPHFDTQDGWRAHFGPRWDVFARRKLTYDPLSILTPGQRIFRKGRAIS
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo06250.1Spo06250.1mRNA


Homology
BLAST of Spo06250.1 vs. NCBI nr
Match: gi|731324348|ref|XP_010672924.1| (PREDICTED: cytokinin dehydrogenase 1-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 998.4 bits (2580), Expect = 4.800e-288
Identity = 477/542 (88.01%), Postives = 514/542 (94.83%), Query Frame = 1

		  

Query: 1   MLSCKFFRQSAEMLLKVFLVLLLNLAEHNINHCSYLPHATPDFTNLSSLELVSSLKKLNL 60
           M SCK  RQSAEMLLKVFLVLLLNLA HNINHCSYLPHA+PDFTNLSSL+LVSSLKKLNL
Sbjct: 1   MFSCKSLRQSAEMLLKVFLVLLLNLAGHNINHCSYLPHASPDFTNLSSLDLVSSLKKLNL 60

Query: 61  NGNFDFENTRRVANDFGNRYHFLPMAVLLPKTVSDISTTIKHVINMGHNSELTVTARGHG 120
            G+FD +NTRRVA DFGNRYHFLPMAVLLPKTVSDISTTIKHV NMGHN ELT+TARGHG
Sbjct: 61  KGHFDLDNTRRVAKDFGNRYHFLPMAVLLPKTVSDISTTIKHVFNMGHNPELTITARGHG 120

Query: 121 HSLQGQSQSHGGIVISMESLEEPKMHVHTGEQPYVDVSGGELWINILHETLKQGLSPKSW 180
           HSLQGQSQ+HGGIVI+MESLEEPKMHV  GE PYV+VSGGELWINILHETLK GL+PKSW
Sbjct: 121 HSLQGQSQNHGGIVINMESLEEPKMHVKAGELPYVEVSGGELWINILHETLKHGLAPKSW 180

Query: 181 TDYLYLTVGGTLSNAGISGQAFRYGPQINNVHRLEIVTGKGDILNCSAKENADLFYGVLG 240
           TDYL+LTVGGTLSNAGISGQAFR+GPQINNV++LE+VTGKGDI+NCS K+NADLFYGVLG
Sbjct: 181 TDYLHLTVGGTLSNAGISGQAFRHGPQINNVYQLEVVTGKGDIVNCSEKQNADLFYGVLG 240

Query: 241 GLGQFGIITRARISLEPAPRSVKWIRVLYSDFNLFTKDQEQLISSDNSFDYIEGFVIINR 300
           GLGQFGIITRARISLEPAP+ VKW RVLYSDFNLFTKDQEQLISSDN+FDYIEGFVIINR
Sbjct: 241 GLGQFGIITRARISLEPAPKMVKWNRVLYSDFNLFTKDQEQLISSDNTFDYIEGFVIINR 300

Query: 301 TGLINNWRSTFSPREPLQASQFSSEGKTLYCLEMAKYFNPEDGNIINQETEHLLSKLNYI 360
           TGL+NNWRSTFSP+EPLQASQFSSEGKTLYCLEMAKYFNPED NIINQ+ E++LSKLNYI
Sbjct: 301 TGLVNNWRSTFSPKEPLQASQFSSEGKTLYCLEMAKYFNPEDANIINQKVEYILSKLNYI 360

Query: 361 SHTLFQSEVSYVNFLNRVHLSEIKLREKGLWDVPHPWLNLLVPKSKIYSFAEEVFGHILT 420
            +T+FQSEVSYV+FLNRVH+SEIKLREKGLWDVPHPWLNLLVPKSKIY+FAEEVFG+ILT
Sbjct: 361 PYTVFQSEVSYVDFLNRVHISEIKLREKGLWDVPHPWLNLLVPKSKIYNFAEEVFGNILT 420

Query: 421 DTSNGPILIYPVNQSRWNHKTSMVTPSEDVFYLVAFLTSAMPSSSGSDGLENILTKNKRI 480
           DTSNGPILIYPVNQSRWN KTS+VTP+EDVFYL+A LTSA+PSSSGSD LENILTKNK I
Sbjct: 421 DTSNGPILIYPVNQSRWNDKTSLVTPAEDVFYLIAILTSALPSSSGSDRLENILTKNKGI 480

Query: 481 LDFCEMAKLGAKQYLPHFDTQDGWRAHFGPRWDVFARRKLTYDPLSILTPGQRIFRKGRA 540
           LDFCE AKLG KQYLPH++TQD WRAHFGP+WDVFARRK TYDPLSILTPGQRIF KGRA
Sbjct: 481 LDFCEKAKLGVKQYLPHYNTQDHWRAHFGPKWDVFARRKSTYDPLSILTPGQRIFYKGRA 540

Query: 541 IS 543
           +S
Sbjct: 541 LS 542

BLAST of Spo06250.1 vs. NCBI nr
Match: gi|731439660|ref|XP_002284560.2| (PREDICTED: cytokinin dehydrogenase 1-like [Vitis vinifera])

HSP 1 Score: 820.5 bits (2118), Expect = 1.800e-234
Identity = 378/540 (70.00%), Postives = 466/540 (86.30%), Query Frame = 1

		  

Query: 3   SCKFFRQSAEMLLKVFLVLLLNLAEHNINHCSYLPHATPDFTNLSSLELVSSLKKLNLNG 62
           S  FFR    +  ++F++L L+   H +N CS LP A P   NL S  ++SSLK ++L+G
Sbjct: 5   SYSFFRLKNTIFFRIFMILFLSSITHRMNLCSNLPLADPSLPNLGSSYVLSSLKTIHLDG 64

Query: 63  NFDFENTRRVANDFGNRYHFLPMAVLLPKTVSDISTTIKHVINMGHNSELTVTARGHGHS 122
           +F+F+N    ANDFGNR+H+LP A+L PK+VSDIS+TIKH+  MG  +ELT+ ARGHGHS
Sbjct: 65  HFNFDNIYHAANDFGNRHHYLPSAILHPKSVSDISSTIKHIFEMGPATELTIAARGHGHS 124

Query: 123 LQGQSQSHGGIVISMESLEEPKMHVHTGEQPYVDVSGGELWINILHETLKQGLSPKSWTD 182
           +QGQ+Q+H G+V++MESL+ PK  VHTG+ PY DVSGGELWINILHE+LK GL+PKSWTD
Sbjct: 125 VQGQAQAHRGVVVNMESLQAPKTIVHTGKMPYADVSGGELWINILHESLKHGLAPKSWTD 184

Query: 183 YLYLTVGGTLSNAGISGQAFRYGPQINNVHRLEIVTGKGDILNCSAKENADLFYGVLGGL 242
           YL+LTVGGTLSNAGISGQAFR+GPQINNV++LE+VTGKGDI+ CS  +NADLFYGVLGGL
Sbjct: 185 YLHLTVGGTLSNAGISGQAFRHGPQINNVYQLEVVTGKGDIITCSETQNADLFYGVLGGL 244

Query: 243 GQFGIITRARISLEPAPRSVKWIRVLYSDFNLFTKDQEQLISSDNSFDYIEGFVIINRTG 302
           GQFGIITRARISLEPAP+ VKWIRVLYS+F++F+KDQE LIS  NSFDYIEGFVIINRTG
Sbjct: 245 GQFGIITRARISLEPAPKMVKWIRVLYSEFSIFSKDQEHLISFKNSFDYIEGFVIINRTG 304

Query: 303 LINNWRSTFSPREPLQASQFSSEGKTLYCLEMAKYFNPEDGNIINQETEHLLSKLNYISH 362
           L+N WRS+F+P EPLQASQF+S+G+TLYCLEMAKYFNP++ +I+NQ  E LLS+L+YI  
Sbjct: 305 LLNTWRSSFNPGEPLQASQFNSDGRTLYCLEMAKYFNPDETHIVNQVIESLLSELSYIPS 364

Query: 363 TLFQSEVSYVNFLNRVHLSEIKLREKGLWDVPHPWLNLLVPKSKIYSFAEEVFGHILTDT 422
           TLF SEV YV+FL+RVH+SEIKLR KGLW+VPHPWLNLL+PKS+I+ FA+EVFG+IL DT
Sbjct: 365 TLFLSEVPYVDFLDRVHVSEIKLRAKGLWEVPHPWLNLLIPKSRIHDFAKEVFGNILRDT 424

Query: 423 SNGPILIYPVNQSRWNHKTSMVTPSEDVFYLVAFLTSAMPSSSGSDGLENILTKNKRILD 482
            NGPILIYPVN+S+WN++TS+VTP ED+FYLVAFL+SA+PSS+G+DGLE+ILT+N+RILD
Sbjct: 425 GNGPILIYPVNKSKWNNRTSLVTPQEDIFYLVAFLSSAVPSSTGTDGLEHILTQNERILD 484

Query: 483 FCEMAKLGAKQYLPHFDTQDGWRAHFGPRWDVFARRKLTYDPLSILTPGQRIFRKGRAIS 542
           FCE A+LG KQYLPH+ TQD W+AHFGP+W+VF +RK TYDPL+IL PGQRIF+K  + S
Sbjct: 485 FCEGARLGMKQYLPHYSTQDKWQAHFGPKWEVFVKRKSTYDPLAILAPGQRIFQKATSFS 544

BLAST of Spo06250.1 vs. NCBI nr
Match: gi|720076342|ref|XP_010279585.1| (PREDICTED: cytokinin dehydrogenase 9-like [Nelumbo nucifera])

HSP 1 Score: 802.7 bits (2072), Expect = 3.800e-229
Identity = 369/532 (69.36%), Postives = 459/532 (86.28%), Query Frame = 1

		  

Query: 6   FFRQSAEMLLKVFLVLLLNLAEHNINHCSYLPHATPDFTNLSSLELVSSLKKLNLNGNFD 65
           F +Q++ +LL+   VL L+     IN  S LP   P  +   S  ++SSLK L L+G F 
Sbjct: 12  FLQQNSILLLRCLAVLFLSCITARINLYSNLPFTIPSVSGSDSPNILSSLKTLTLDGYFS 71

Query: 66  FENTRRVANDFGNRYHFLPMAVLLPKTVSDISTTIKHVINMGHNSELTVTARGHGHSLQG 125
           F+     A DFGNRY FLP A+L PK+VSDI+TTIKH++ +G +S+LT+ ARGHGHSLQG
Sbjct: 72  FDELHHAARDFGNRYQFLPSAILRPKSVSDIATTIKHILQLGPSSKLTIAARGHGHSLQG 131

Query: 126 QSQSHGGIVISMESLEEPKMHVHTGEQPYVDVSGGELWINILHETLKQGLSPKSWTDYLY 185
           Q+Q+HGG+VI+MESL+EPKM VHTGE PYVDVSGGELWINILHE+LK GL+PKSWTDYL+
Sbjct: 132 QAQAHGGVVINMESLQEPKMQVHTGELPYVDVSGGELWINILHESLKYGLAPKSWTDYLH 191

Query: 186 LTVGGTLSNAGISGQAFRYGPQINNVHRLEIVTGKGDILNCSAKENADLFYGVLGGLGQF 245
           LTVGGTLSNAGISGQAFR+GPQI+NV++LE+VTGKG++++CS K+NADLF+GVLGGLGQF
Sbjct: 192 LTVGGTLSNAGISGQAFRHGPQISNVYQLEVVTGKGEVVSCSEKQNADLFHGVLGGLGQF 251

Query: 246 GIITRARISLEPAPRSVKWIRVLYSDFNLFTKDQEQLISSDNSFDYIEGFVIINRTGLIN 305
           GII RARI+LEPAP+ VKWIRVLYSDF++FT+DQE LIS++N+FDYIEGFVIINRTGL+N
Sbjct: 252 GIIARARIALEPAPKMVKWIRVLYSDFSMFTRDQEYLISAENTFDYIEGFVIINRTGLLN 311

Query: 306 NWRSTFSPREPLQASQFSSEGKTLYCLEMAKYFNPEDGNIINQETEHLLSKLNYISHTLF 365
           NWRS F+P++PL+ASQF S+G+TL+CLEM K FNP++ +++NQE E LLS+LNYI  TLF
Sbjct: 312 NWRSFFNPQDPLKASQFKSDGRTLFCLEMTKNFNPDETDVMNQEVESLLSQLNYIPSTLF 371

Query: 366 QSEVSYVNFLNRVHLSEIKLREKGLWDVPHPWLNLLVPKSKIYSFAEEVFGHILTDTSNG 425
            SEVSYV FL+RVH+SEIKLR KGLW+VPHPWLNLL+P+S+I+ FAEEVFG ILTDTSNG
Sbjct: 372 ASEVSYVEFLDRVHMSEIKLRSKGLWEVPHPWLNLLIPRSRIHDFAEEVFGKILTDTSNG 431

Query: 426 PILIYPVNQSRWNHKTSMVTPSEDVFYLVAFLTSAMPSSSGSDGLENILTKNKRILDFCE 485
           P+LIYP+++S+W+ +TS V P+ED+FYLVAFL+SA+PSS+G+DGLE+ILT+NKRILDFCE
Sbjct: 432 PVLIYPLHRSKWDSRTSTVIPNEDIFYLVAFLSSAVPSSTGTDGLEHILTQNKRILDFCE 491

Query: 486 MAKLGAKQYLPHFDTQDGWRAHFGPRWDVFARRKLTYDPLSILTPGQRIFRK 538
            A+LG KQYLPH+ TQ+ WRAHFGPRW+ F +RK  YDPL+IL PGQ+IFRK
Sbjct: 492 TARLGIKQYLPHYTTQEEWRAHFGPRWETFVQRKSAYDPLAILAPGQKIFRK 543

BLAST of Spo06250.1 vs. NCBI nr
Match: gi|567856976|ref|XP_006421171.1| (hypothetical protein CICLE_v10006491mg [Citrus clementina])

HSP 1 Score: 783.1 bits (2021), Expect = 3.100e-223
Identity = 368/533 (69.04%), Postives = 449/533 (84.24%), Query Frame = 1

		  

Query: 6   FFRQSAEMLLKVFLVLLLNLAEHNINHCSYLPHATPDF-TNLSSLELVSSLKKLNLNGNF 65
           F RQ+  +  ++F +L  +      N CS L  AT    T+ +S +   SLKKL L+G+F
Sbjct: 8   FLRQNKPLFFRIFFILFFSCIPDTANLCSNLSLATTAVQTHSASSDNHFSLKKLTLDGHF 67

Query: 66  DFENTRRVANDFGNRYHFLPMAVLLPKTVSDISTTIKHVINMGHNSELTVTARGHGHSLQ 125
            FEN    A DFGN YHF P AVL PK++SDIS+TIKH+ N+G  S+LTV ARGHGHSLQ
Sbjct: 68  SFENIHYAAKDFGNTYHFHPSAVLYPKSISDISSTIKHIFNIGTASQLTVAARGHGHSLQ 127

Query: 126 GQSQSHGGIVISMESLEEPKMHVHTGEQPYVDVSGGELWINILHETLKQGLSPKSWTDYL 185
           GQ+Q+H G+VI+M SL++P M VHTGE PYVDVSGGELWINILHETLK GL+PKSWTDYL
Sbjct: 128 GQAQAHQGVVINMPSLQKPVMKVHTGELPYVDVSGGELWINILHETLKHGLAPKSWTDYL 187

Query: 186 YLTVGGTLSNAGISGQAFRYGPQINNVHRLEIVTGKGDILNCSAKENADLFYGVLGGLGQ 245
           +LTVGGTLSNAGISGQAF +GPQINNV++LE+VTGKGD+L CS ++NADLF+GVLGGLGQ
Sbjct: 188 HLTVGGTLSNAGISGQAFLHGPQINNVYQLEVVTGKGDVLTCSEQQNADLFHGVLGGLGQ 247

Query: 246 FGIITRARISLEPAPRSVKWIRVLYSDFNLFTKDQEQLISSDNSFDYIEGFVIINRTGLI 305
           FGIITRARISLEPAP+ V+WIRVLYS+F++FTKDQE LISS++SFDYIEGFVIINRTGL+
Sbjct: 248 FGIITRARISLEPAPKMVRWIRVLYSEFSIFTKDQEHLISSESSFDYIEGFVIINRTGLL 307

Query: 306 NNWRSTFSPREPLQASQFSSEGKTLYCLEMAKYFNPEDGNIINQETEHLLSKLNYISHTL 365
           NNWRS+F+PR+PLQAS+F+S+GK LYCLEMAK+FNP++  I++++TE LLSKL++I  TL
Sbjct: 308 NNWRSSFNPRDPLQASKFNSDGKVLYCLEMAKHFNPDEAGIMDKKTESLLSKLSFIPSTL 367

Query: 366 FQSEVSYVNFLNRVHLSEIKLREKGLWDVPHPWLNLLVPKSKIYSFAEEVFGHILTDTSN 425
           FQS+VSYV+FL+RVH+SEIKLREKGLW+VPHPWLNLL+PKSKI  FA++VFG IL D SN
Sbjct: 368 FQSDVSYVDFLDRVHVSEIKLREKGLWEVPHPWLNLLIPKSKISDFAQQVFGEILKDNSN 427

Query: 426 GPILIYPVNQSRWNHKTSMVTPSEDVFYLVAFLTSAMPSSSGSDGLENILTKNKRILDFC 485
           GP+LIYPVN+S+WN KTS VTP ED+FYLVAFL+SA+PSS G+DGLE+I  +N+RILD  
Sbjct: 428 GPVLIYPVNKSKWNRKTSFVTPDEDIFYLVAFLSSALPSSRGTDGLEHINNQNQRILDVS 487

Query: 486 EMAKLGAKQYLPHFDTQDGWRAHFGPRWDVFARRKLTYDPLSILTPGQRIFRK 538
             A LG KQYLPH+ TQ  W+AHFGP+W +F +RK  YDPL+IL PGQRIF+K
Sbjct: 488 TRANLGIKQYLPHYSTQKEWQAHFGPQWKLFVQRKYAYDPLAILAPGQRIFQK 540

BLAST of Spo06250.1 vs. NCBI nr
Match: gi|568876876|ref|XP_006491496.1| (PREDICTED: cytokinin dehydrogenase 1-like [Citrus sinensis])

HSP 1 Score: 782.7 bits (2020), Expect = 4.100e-223
Identity = 369/533 (69.23%), Postives = 449/533 (84.24%), Query Frame = 1

		  

Query: 6   FFRQSAEMLLKVFLVLLLNLAEHNINHCSYLPHATPDF-TNLSSLELVSSLKKLNLNGNF 65
           F +Q+  +  ++F +L  +      N CS L  AT    T+ +S +   SLKKL L+G+F
Sbjct: 12  FLKQNKPLFFRIFFILFFSCIPDTANLCSNLSLATTAVQTHSASSDNHFSLKKLTLDGHF 71

Query: 66  DFENTRRVANDFGNRYHFLPMAVLLPKTVSDISTTIKHVINMGHNSELTVTARGHGHSLQ 125
            FEN    A DFGN YHF P AVL PK+VSDIS+TIKH+ N+G  S+LTV ARGHGHSLQ
Sbjct: 72  SFENIHYAAKDFGNTYHFHPSAVLYPKSVSDISSTIKHIFNIGTASQLTVAARGHGHSLQ 131

Query: 126 GQSQSHGGIVISMESLEEPKMHVHTGEQPYVDVSGGELWINILHETLKQGLSPKSWTDYL 185
           GQ+Q+H G+VI+M SL+EP M VHTGE PYVDVSGGELWINILHETLK GL+PKSWTDYL
Sbjct: 132 GQAQAHQGVVINMPSLQEPVMKVHTGELPYVDVSGGELWINILHETLKNGLAPKSWTDYL 191

Query: 186 YLTVGGTLSNAGISGQAFRYGPQINNVHRLEIVTGKGDILNCSAKENADLFYGVLGGLGQ 245
           +LTVGGTLSNAGISGQAF +GPQINNV++LE+VTGKGD+L CS ++NADLF+GVLGGLGQ
Sbjct: 192 HLTVGGTLSNAGISGQAFLHGPQINNVYKLEVVTGKGDVLTCSEQQNADLFHGVLGGLGQ 251

Query: 246 FGIITRARISLEPAPRSVKWIRVLYSDFNLFTKDQEQLISSDNSFDYIEGFVIINRTGLI 305
           FGIITRARISLEPAP+ V+WIRVLYS+F++FTKDQE LISS++SFDYIEGFVIINRTGL+
Sbjct: 252 FGIITRARISLEPAPKMVRWIRVLYSEFSIFTKDQEHLISSESSFDYIEGFVIINRTGLL 311

Query: 306 NNWRSTFSPREPLQASQFSSEGKTLYCLEMAKYFNPEDGNIINQETEHLLSKLNYISHTL 365
           NNWRS+F+PR+PLQAS+F+S+GK LYCLEMAK+FNP++  I++++TE LLSKL++I  TL
Sbjct: 312 NNWRSSFNPRDPLQASKFNSDGKVLYCLEMAKHFNPDEAGIMDKKTESLLSKLSFIPSTL 371

Query: 366 FQSEVSYVNFLNRVHLSEIKLREKGLWDVPHPWLNLLVPKSKIYSFAEEVFGHILTDTSN 425
           FQS+VSYV+FL+RVH+SEIKLREKGLW+VPHPWLNLL+PKSKI  FA++VFG IL D SN
Sbjct: 372 FQSDVSYVDFLDRVHVSEIKLREKGLWEVPHPWLNLLIPKSKISDFAQQVFGEILKDNSN 431

Query: 426 GPILIYPVNQSRWNHKTSMVTPSEDVFYLVAFLTSAMPSSSGSDGLENILTKNKRILDFC 485
           GPILIYPVN+S+WN KTS VTP ED+FYLVAFL+SA+PSS G+DGLE+I  +++RILD  
Sbjct: 432 GPILIYPVNKSKWNRKTSFVTPDEDIFYLVAFLSSALPSSRGTDGLEHINNQHQRILDVS 491

Query: 486 EMAKLGAKQYLPHFDTQDGWRAHFGPRWDVFARRKLTYDPLSILTPGQRIFRK 538
             A LG KQYLPH+ TQ  W+AHFGP+W +F +RK  YDPL+IL PGQRIF+K
Sbjct: 492 TRANLGIKQYLPHYSTQKEWQAHFGPQWKLFVQRKYAYDPLAILAPGQRIFQK 544

BLAST of Spo06250.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CSR7_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g062100 PE=4 SV=1)

HSP 1 Score: 998.4 bits (2580), Expect = 3.300e-288
Identity = 477/542 (88.01%), Postives = 514/542 (94.83%), Query Frame = 1

		  

Query: 1   MLSCKFFRQSAEMLLKVFLVLLLNLAEHNINHCSYLPHATPDFTNLSSLELVSSLKKLNL 60
           M SCK  RQSAEMLLKVFLVLLLNLA HNINHCSYLPHA+PDFTNLSSL+LVSSLKKLNL
Sbjct: 1   MFSCKSLRQSAEMLLKVFLVLLLNLAGHNINHCSYLPHASPDFTNLSSLDLVSSLKKLNL 60

Query: 61  NGNFDFENTRRVANDFGNRYHFLPMAVLLPKTVSDISTTIKHVINMGHNSELTVTARGHG 120
            G+FD +NTRRVA DFGNRYHFLPMAVLLPKTVSDISTTIKHV NMGHN ELT+TARGHG
Sbjct: 61  KGHFDLDNTRRVAKDFGNRYHFLPMAVLLPKTVSDISTTIKHVFNMGHNPELTITARGHG 120

Query: 121 HSLQGQSQSHGGIVISMESLEEPKMHVHTGEQPYVDVSGGELWINILHETLKQGLSPKSW 180
           HSLQGQSQ+HGGIVI+MESLEEPKMHV  GE PYV+VSGGELWINILHETLK GL+PKSW
Sbjct: 121 HSLQGQSQNHGGIVINMESLEEPKMHVKAGELPYVEVSGGELWINILHETLKHGLAPKSW 180

Query: 181 TDYLYLTVGGTLSNAGISGQAFRYGPQINNVHRLEIVTGKGDILNCSAKENADLFYGVLG 240
           TDYL+LTVGGTLSNAGISGQAFR+GPQINNV++LE+VTGKGDI+NCS K+NADLFYGVLG
Sbjct: 181 TDYLHLTVGGTLSNAGISGQAFRHGPQINNVYQLEVVTGKGDIVNCSEKQNADLFYGVLG 240

Query: 241 GLGQFGIITRARISLEPAPRSVKWIRVLYSDFNLFTKDQEQLISSDNSFDYIEGFVIINR 300
           GLGQFGIITRARISLEPAP+ VKW RVLYSDFNLFTKDQEQLISSDN+FDYIEGFVIINR
Sbjct: 241 GLGQFGIITRARISLEPAPKMVKWNRVLYSDFNLFTKDQEQLISSDNTFDYIEGFVIINR 300

Query: 301 TGLINNWRSTFSPREPLQASQFSSEGKTLYCLEMAKYFNPEDGNIINQETEHLLSKLNYI 360
           TGL+NNWRSTFSP+EPLQASQFSSEGKTLYCLEMAKYFNPED NIINQ+ E++LSKLNYI
Sbjct: 301 TGLVNNWRSTFSPKEPLQASQFSSEGKTLYCLEMAKYFNPEDANIINQKVEYILSKLNYI 360

Query: 361 SHTLFQSEVSYVNFLNRVHLSEIKLREKGLWDVPHPWLNLLVPKSKIYSFAEEVFGHILT 420
            +T+FQSEVSYV+FLNRVH+SEIKLREKGLWDVPHPWLNLLVPKSKIY+FAEEVFG+ILT
Sbjct: 361 PYTVFQSEVSYVDFLNRVHISEIKLREKGLWDVPHPWLNLLVPKSKIYNFAEEVFGNILT 420

Query: 421 DTSNGPILIYPVNQSRWNHKTSMVTPSEDVFYLVAFLTSAMPSSSGSDGLENILTKNKRI 480
           DTSNGPILIYPVNQSRWN KTS+VTP+EDVFYL+A LTSA+PSSSGSD LENILTKNK I
Sbjct: 421 DTSNGPILIYPVNQSRWNDKTSLVTPAEDVFYLIAILTSALPSSSGSDRLENILTKNKGI 480

Query: 481 LDFCEMAKLGAKQYLPHFDTQDGWRAHFGPRWDVFARRKLTYDPLSILTPGQRIFRKGRA 540
           LDFCE AKLG KQYLPH++TQD WRAHFGP+WDVFARRK TYDPLSILTPGQRIF KGRA
Sbjct: 481 LDFCEKAKLGVKQYLPHYNTQDHWRAHFGPKWDVFARRKSTYDPLSILTPGQRIFYKGRA 540

Query: 541 IS 543
           +S
Sbjct: 541 LS 542

BLAST of Spo06250.1 vs. UniProtKB/TrEMBL
Match: D7TSA7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_00s0252g00040 PE=4 SV=1)

HSP 1 Score: 820.5 bits (2118), Expect = 1.200e-234
Identity = 378/540 (70.00%), Postives = 466/540 (86.30%), Query Frame = 1

		  

Query: 3   SCKFFRQSAEMLLKVFLVLLLNLAEHNINHCSYLPHATPDFTNLSSLELVSSLKKLNLNG 62
           S  FFR    +  ++F++L L+   H +N CS LP A P   NL S  ++SSLK ++L+G
Sbjct: 5   SYSFFRLKNTIFFRIFMILFLSSITHRMNLCSNLPLADPSLPNLGSSYVLSSLKTIHLDG 64

Query: 63  NFDFENTRRVANDFGNRYHFLPMAVLLPKTVSDISTTIKHVINMGHNSELTVTARGHGHS 122
           +F+F+N    ANDFGNR+H+LP A+L PK+VSDIS+TIKH+  MG  +ELT+ ARGHGHS
Sbjct: 65  HFNFDNIYHAANDFGNRHHYLPSAILHPKSVSDISSTIKHIFEMGPATELTIAARGHGHS 124

Query: 123 LQGQSQSHGGIVISMESLEEPKMHVHTGEQPYVDVSGGELWINILHETLKQGLSPKSWTD 182
           +QGQ+Q+H G+V++MESL+ PK  VHTG+ PY DVSGGELWINILHE+LK GL+PKSWTD
Sbjct: 125 VQGQAQAHRGVVVNMESLQAPKTIVHTGKMPYADVSGGELWINILHESLKHGLAPKSWTD 184

Query: 183 YLYLTVGGTLSNAGISGQAFRYGPQINNVHRLEIVTGKGDILNCSAKENADLFYGVLGGL 242
           YL+LTVGGTLSNAGISGQAFR+GPQINNV++LE+VTGKGDI+ CS  +NADLFYGVLGGL
Sbjct: 185 YLHLTVGGTLSNAGISGQAFRHGPQINNVYQLEVVTGKGDIITCSETQNADLFYGVLGGL 244

Query: 243 GQFGIITRARISLEPAPRSVKWIRVLYSDFNLFTKDQEQLISSDNSFDYIEGFVIINRTG 302
           GQFGIITRARISLEPAP+ VKWIRVLYS+F++F+KDQE LIS  NSFDYIEGFVIINRTG
Sbjct: 245 GQFGIITRARISLEPAPKMVKWIRVLYSEFSIFSKDQEHLISFKNSFDYIEGFVIINRTG 304

Query: 303 LINNWRSTFSPREPLQASQFSSEGKTLYCLEMAKYFNPEDGNIINQETEHLLSKLNYISH 362
           L+N WRS+F+P EPLQASQF+S+G+TLYCLEMAKYFNP++ +I+NQ  E LLS+L+YI  
Sbjct: 305 LLNTWRSSFNPGEPLQASQFNSDGRTLYCLEMAKYFNPDETHIVNQVIESLLSELSYIPS 364

Query: 363 TLFQSEVSYVNFLNRVHLSEIKLREKGLWDVPHPWLNLLVPKSKIYSFAEEVFGHILTDT 422
           TLF SEV YV+FL+RVH+SEIKLR KGLW+VPHPWLNLL+PKS+I+ FA+EVFG+IL DT
Sbjct: 365 TLFLSEVPYVDFLDRVHVSEIKLRAKGLWEVPHPWLNLLIPKSRIHDFAKEVFGNILRDT 424

Query: 423 SNGPILIYPVNQSRWNHKTSMVTPSEDVFYLVAFLTSAMPSSSGSDGLENILTKNKRILD 482
            NGPILIYPVN+S+WN++TS+VTP ED+FYLVAFL+SA+PSS+G+DGLE+ILT+N+RILD
Sbjct: 425 GNGPILIYPVNKSKWNNRTSLVTPQEDIFYLVAFLSSAVPSSTGTDGLEHILTQNERILD 484

Query: 483 FCEMAKLGAKQYLPHFDTQDGWRAHFGPRWDVFARRKLTYDPLSILTPGQRIFRKGRAIS 542
           FCE A+LG KQYLPH+ TQD W+AHFGP+W+VF +RK TYDPL+IL PGQRIF+K  + S
Sbjct: 485 FCEGARLGMKQYLPHYSTQDKWQAHFGPKWEVFVKRKSTYDPLAILAPGQRIFQKATSFS 544

BLAST of Spo06250.1 vs. UniProtKB/TrEMBL
Match: V4SBS5_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10006491mg PE=4 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 2.200e-223
Identity = 368/533 (69.04%), Postives = 449/533 (84.24%), Query Frame = 1

		  

Query: 6   FFRQSAEMLLKVFLVLLLNLAEHNINHCSYLPHATPDF-TNLSSLELVSSLKKLNLNGNF 65
           F RQ+  +  ++F +L  +      N CS L  AT    T+ +S +   SLKKL L+G+F
Sbjct: 8   FLRQNKPLFFRIFFILFFSCIPDTANLCSNLSLATTAVQTHSASSDNHFSLKKLTLDGHF 67

Query: 66  DFENTRRVANDFGNRYHFLPMAVLLPKTVSDISTTIKHVINMGHNSELTVTARGHGHSLQ 125
            FEN    A DFGN YHF P AVL PK++SDIS+TIKH+ N+G  S+LTV ARGHGHSLQ
Sbjct: 68  SFENIHYAAKDFGNTYHFHPSAVLYPKSISDISSTIKHIFNIGTASQLTVAARGHGHSLQ 127

Query: 126 GQSQSHGGIVISMESLEEPKMHVHTGEQPYVDVSGGELWINILHETLKQGLSPKSWTDYL 185
           GQ+Q+H G+VI+M SL++P M VHTGE PYVDVSGGELWINILHETLK GL+PKSWTDYL
Sbjct: 128 GQAQAHQGVVINMPSLQKPVMKVHTGELPYVDVSGGELWINILHETLKHGLAPKSWTDYL 187

Query: 186 YLTVGGTLSNAGISGQAFRYGPQINNVHRLEIVTGKGDILNCSAKENADLFYGVLGGLGQ 245
           +LTVGGTLSNAGISGQAF +GPQINNV++LE+VTGKGD+L CS ++NADLF+GVLGGLGQ
Sbjct: 188 HLTVGGTLSNAGISGQAFLHGPQINNVYQLEVVTGKGDVLTCSEQQNADLFHGVLGGLGQ 247

Query: 246 FGIITRARISLEPAPRSVKWIRVLYSDFNLFTKDQEQLISSDNSFDYIEGFVIINRTGLI 305
           FGIITRARISLEPAP+ V+WIRVLYS+F++FTKDQE LISS++SFDYIEGFVIINRTGL+
Sbjct: 248 FGIITRARISLEPAPKMVRWIRVLYSEFSIFTKDQEHLISSESSFDYIEGFVIINRTGLL 307

Query: 306 NNWRSTFSPREPLQASQFSSEGKTLYCLEMAKYFNPEDGNIINQETEHLLSKLNYISHTL 365
           NNWRS+F+PR+PLQAS+F+S+GK LYCLEMAK+FNP++  I++++TE LLSKL++I  TL
Sbjct: 308 NNWRSSFNPRDPLQASKFNSDGKVLYCLEMAKHFNPDEAGIMDKKTESLLSKLSFIPSTL 367

Query: 366 FQSEVSYVNFLNRVHLSEIKLREKGLWDVPHPWLNLLVPKSKIYSFAEEVFGHILTDTSN 425
           FQS+VSYV+FL+RVH+SEIKLREKGLW+VPHPWLNLL+PKSKI  FA++VFG IL D SN
Sbjct: 368 FQSDVSYVDFLDRVHVSEIKLREKGLWEVPHPWLNLLIPKSKISDFAQQVFGEILKDNSN 427

Query: 426 GPILIYPVNQSRWNHKTSMVTPSEDVFYLVAFLTSAMPSSSGSDGLENILTKNKRILDFC 485
           GP+LIYPVN+S+WN KTS VTP ED+FYLVAFL+SA+PSS G+DGLE+I  +N+RILD  
Sbjct: 428 GPVLIYPVNKSKWNRKTSFVTPDEDIFYLVAFLSSALPSSRGTDGLEHINNQNQRILDVS 487

Query: 486 EMAKLGAKQYLPHFDTQDGWRAHFGPRWDVFARRKLTYDPLSILTPGQRIFRK 538
             A LG KQYLPH+ TQ  W+AHFGP+W +F +RK  YDPL+IL PGQRIF+K
Sbjct: 488 TRANLGIKQYLPHYSTQKEWQAHFGPQWKLFVQRKYAYDPLAILAPGQRIFQK 540

BLAST of Spo06250.1 vs. UniProtKB/TrEMBL
Match: B9H9J6_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0006s04650g PE=4 SV=2)

HSP 1 Score: 771.5 bits (1991), Expect = 6.600e-220
Identity = 364/530 (68.68%), Postives = 434/530 (81.89%), Query Frame = 1

		  

Query: 8   RQSAEMLLKVFLVLLLNLAEHNINHCSYLPHATPDFTNLSSLELVSSLKKLNLNGNFDFE 67
           RQ+  + L   ++L        IN CS     +P      S   +SSL+ L L+G F F+
Sbjct: 10  RQNNLLFLVFIMILFFYSIPDKINLCSNQSSVSPSVIPYKSSSNISSLETLKLDGYFSFD 69

Query: 68  NTRRVANDFGNRYHFLPMAVLLPKTVSDISTTIKHVINMGHNSELTVTARGHGHSLQGQS 127
           N    A DFGN YHFLP AVL PK+VSDIS TIKH+  MG  S+LTV ARGH HSLQGQ+
Sbjct: 70  NIDYAAKDFGNMYHFLPSAVLHPKSVSDISNTIKHIFKMGSTSQLTVAARGHSHSLQGQA 129

Query: 128 QSHGGIVISMESLEEPKMHVHTGEQPYVDVSGGELWINILHETLKQGLSPKSWTDYLYLT 187
           Q+H GIVI+MESL+ P+M VHTGE PYVD SG ELWINILHETLK GL+PKSWTDYL+LT
Sbjct: 130 QAHQGIVINMESLQGPEMQVHTGELPYVDASGSELWINILHETLKYGLAPKSWTDYLHLT 189

Query: 188 VGGTLSNAGISGQAFRYGPQINNVHRLEIVTGKGDILNCSAKENADLFYGVLGGLGQFGI 247
           VGGTLSNAGISGQAF++GPQINN+++LE+VTGKG+ + CS  +NADLFYGVLGGLGQFGI
Sbjct: 190 VGGTLSNAGISGQAFKHGPQINNIYQLEVVTGKGEAVTCSENKNADLFYGVLGGLGQFGI 249

Query: 248 ITRARISLEPAPRSVKWIRVLYSDFNLFTKDQEQLISSDNSFDYIEGFVIINRTGLINNW 307
           ITRARISLEPAP+ VKWIR LY +F+ F+ DQE LIS  NSFDYIEG VIINRTGL+NNW
Sbjct: 250 ITRARISLEPAPKMVKWIRALYDEFSKFSSDQEHLISK-NSFDYIEGLVIINRTGLLNNW 309

Query: 308 RSTFSPREPLQASQFSSEGKTLYCLEMAKYFNPEDGNIINQETEHLLSKLNYISHTLFQS 367
           RS+F+P++PLQASQF SEGKTLYCLE+AKYFNP + + +NQETE LLS+L+YI  TLF S
Sbjct: 310 RSSFNPKDPLQASQFISEGKTLYCLEIAKYFNPNESDAMNQETESLLSELSYIPSTLFLS 369

Query: 368 EVSYVNFLNRVHLSEIKLREKGLWDVPHPWLNLLVPKSKIYSFAEEVFGHILTDTSNGPI 427
           EVSYV FL+RVHLSEIKLR KGLWD+PHPWLNLL+PK+KI+ FA+EVFG+ILTD+SNGPI
Sbjct: 370 EVSYVEFLDRVHLSEIKLRAKGLWDIPHPWLNLLIPKNKIFEFAQEVFGNILTDSSNGPI 429

Query: 428 LIYPVNQSRWNHKTSMVTPSEDVFYLVAFLTSAMPSSSGSDGLENILTKNKRILDFCEMA 487
           LIYPVN+S+W+++TS++TP ED FYLVAFL+SAMPSS+G DGL +IL +N+RIL FC   
Sbjct: 430 LIYPVNKSKWDNRTSLITPEEDTFYLVAFLSSAMPSSTGRDGLFHILAQNQRILGFCSST 489

Query: 488 KLGAKQYLPHFDTQDGWRAHFGPRWDVFARRKLTYDPLSILTPGQRIFRK 538
            LGAKQYLPH+ TQ+ W+ HFGP+W+VF  RK TYDPL+IL PGQRIFR+
Sbjct: 490 SLGAKQYLPHYSTQEEWQTHFGPKWEVFVLRKSTYDPLAILAPGQRIFRR 538

BLAST of Spo06250.1 vs. UniProtKB/TrEMBL
Match: A0A061FLK6_THECC (Cytokinin oxidase/dehydrogenase 6 isoform 1 OS=Theobroma cacao GN=TCM_042367 PE=4 SV=1)

HSP 1 Score: 769.6 bits (1986), Expect = 2.500e-219
Identity = 359/537 (66.85%), Postives = 452/537 (84.17%), Query Frame = 1

		  

Query: 6   FFRQSAEMLLKVFLVLLLNLAEHNINHCSYLPHATPDFTNLSSLELVSSLKKLNLNGNFD 65
           F R++  + L+ F++L L+     IN C   P             + SSLK L+++G+F 
Sbjct: 8   FPRKTNMLFLRSFMILFLSCITIKINLC--FP------------SIPSSLKTLSIDGHFK 67

Query: 66  FENTRRVANDFGNRYHFLPMAVLLPKTVSDISTTIKHVINMGHNSELTVTARGHGHSLQG 125
           FE     A DFGNRY FLP+AVL PK+VS I+TT+KH+  MG  SELTV ARGHGHSLQG
Sbjct: 68  FEEVDHAAKDFGNRYRFLPLAVLHPKSVSGIATTVKHIWQMGPRSELTVAARGHGHSLQG 127

Query: 126 QSQSHGGIVISMESLEEPKMHVHTGEQPYVDVSGGELWINILHETLKQGLSPKSWTDYLY 185
           Q+Q+H G+VI+MESL+ PKM VH G+ PYVDVSGGELWINIL E+L+ GL+PKSWTDYL+
Sbjct: 128 QAQAHRGVVINMESLQGPKMQVHAGKFPYVDVSGGELWINILRESLRHGLAPKSWTDYLH 187

Query: 186 LTVGGTLSNAGISGQAFRYGPQINNVHRLEIVTGKGDILNCSAKENADLFYGVLGGLGQF 245
           LTVGGTLSNAGISGQAFR+GPQI+NVH+LE+VTGKG+++NCS K+N+DLFY VLGGLGQF
Sbjct: 188 LTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGEVVNCSEKQNSDLFYSVLGGLGQF 247

Query: 246 GIITRARISLEPAPRSVKWIRVLYSDFNLFTKDQEQLISSDNSFDYIEGFVIINRTGLIN 305
           GIITRARISLEPAP  VKWIRVLY+DF  F +DQE+LIS +++FDYIEGFVIINRTGL+N
Sbjct: 248 GIITRARISLEPAPEMVKWIRVLYTDFATFARDQEKLISGESTFDYIEGFVIINRTGLLN 307

Query: 306 NWRSTFSPREPLQASQFSSEGKTLYCLEMAKYFNPEDGNIINQETEHLLSKLNYISHTLF 365
           NWRS+F+P++P+QASQF S+G+TL+CLE+AKYFNPE+  ++NQE ++ LS+LN+I  TLF
Sbjct: 308 NWRSSFNPQDPVQASQFKSDGRTLFCLELAKYFNPEEMAVVNQEIKNSLSQLNHIPSTLF 367

Query: 366 QSEVSYVNFLNRVHLSEIKLREKGLWDVPHPWLNLLVPKSKIYSFAEEVFGHILTDTSNG 425
            SEV Y+ FL+RVH+SEIKLR KGLW+VPHPWLNLLVP+SK++SFA+EVFG+ILTDTSNG
Sbjct: 368 MSEVPYIEFLDRVHISEIKLRSKGLWEVPHPWLNLLVPRSKVHSFAQEVFGNILTDTSNG 427

Query: 426 PILIYPVNQSRWNHKTSMVTPSEDVFYLVAFLTSAMPSSSGSDGLENILTKNKRILDFCE 485
           PILIYPVN+S+W+++TS V P EDVFYLVAFL+SA+PSS+G+DGL++IL +NKRIL+FCE
Sbjct: 428 PILIYPVNKSKWDNRTSAVIPEEDVFYLVAFLSSAVPSSTGTDGLDHILIQNKRILEFCE 487

Query: 486 MAKLGAKQYLPHFDTQDGWRAHFGPRWDVFARRKLTYDPLSILTPGQRIFRKGRAIS 543
           +A+LG KQYLPH+ TQ  W+AH+GP+W++F +RK TYDPL++L PGQRIF+K  A S
Sbjct: 488 IARLGVKQYLPHYSTQGEWQAHYGPQWELFVQRKSTYDPLALLAPGQRIFQKPIAYS 530

BLAST of Spo06250.1 vs. ExPASy Swiss-Prot
Match: CKX1_ARATH (Cytokinin dehydrogenase 1 OS=Arabidopsis thaliana GN=CKX1 PE=1 SV=1)

HSP 1 Score: 726.9 bits (1875), Expect = 1.700e-208
Identity = 336/536 (62.69%), Postives = 429/536 (80.04%), Query Frame = 1

		  

Query: 3   SCKFFRQSAEMLLKVFLVLLLNLAEHNINHCSYLPHATP-DFTNLSSLELVSSLKKLNLN 62
           S +F RQ+ +  L +F++L+L+      N CS    +TP +  + +  ++ SSL  L+L 
Sbjct: 6   SLRFHRQNNKTFLGIFMILVLSCIPGRTNLCSNHSVSTPKELPSSNPSDIRSSLVSLDLE 65

Query: 63  GNFDFENTRRVANDFGNRYHFLPMAVLLPKTVSDISTTIKHVINMGHNSELTVTARGHGH 122
           G   F++   VA DFGNRY   P+A+L P++V DIS+ +KH++++G  S LTV ARGHGH
Sbjct: 66  GYISFDDVHNVAKDFGNRYQLPPLAILHPRSVFDISSMMKHIVHLGSTSNLTVAARGHGH 125

Query: 123 SLQGQSQSHGGIVISMESLEEPKMHVHTGEQPYVDVSGGELWINILHETLKQGLSPKSWT 182
           SLQGQ+ +H G+VI MESL  P + ++ G+QPYVDVSGGE+WINIL ETLK GLSPKSWT
Sbjct: 126 SLQGQALAHQGVVIKMESLRSPDIRIYKGKQPYVDVSGGEIWINILRETLKYGLSPKSWT 185

Query: 183 DYLYLTVGGTLSNAGISGQAFRYGPQINNVHRLEIVTGKGDILNCSAKENADLFYGVLGG 242
           DYL+LTVGGTLSNAGISGQAF++GPQINNV++LEIVTGKG+++ CS K N++LF+ VLGG
Sbjct: 186 DYLHLTVGGTLSNAGISGQAFKHGPQINNVYQLEIVTGKGEVVTCSEKRNSELFFSVLGG 245

Query: 243 LGQFGIITRARISLEPAPRSVKWIRVLYSDFNLFTKDQEQLISSDNSFDYIEGFVIINRT 302
           LGQFGIITRARISLEPAP  VKWIRVLYSDF+ F++DQE LIS + +FDY+EGFVIINRT
Sbjct: 246 LGQFGIITRARISLEPAPHMVKWIRVLYSDFSAFSRDQEYLISKEKTFDYVEGFVIINRT 305

Query: 303 GLINNWRSTFSPREPLQASQFSSEGKTLYCLEMAKYFNPEDGNIINQETEHLLSKLNYIS 362
            L+NNWRS+FSP +  QAS+F S+GKTLYCLE+ KYFNPE+ + ++QET  LLS+LNYI 
Sbjct: 306 DLLNNWRSSFSPNDSTQASRFKSDGKTLYCLEVVKYFNPEEASSMDQETGKLLSELNYIP 365

Query: 363 HTLFQSEVSYVNFLNRVHLSEIKLREKGLWDVPHPWLNLLVPKSKIYSFAEEVFGHILTD 422
            TLF SEV Y+ FL+RVH++E KLR KGLW+VPHPWLNLL+PKS IY FA EVF +ILT 
Sbjct: 366 STLFSSEVPYIEFLDRVHIAERKLRAKGLWEVPHPWLNLLIPKSSIYQFATEVFNNILTS 425

Query: 423 TSNGPILIYPVNQSRWNHKTSMVTPSEDVFYLVAFLTSAMPSSSGSDGLENILTKNKRIL 482
            +NGPILIYPVNQS+W   TS++TP+ED+FYLVAFL SA+P+SSG + LE +L +N+R++
Sbjct: 426 NNNGPILIYPVNQSKWKKHTSLITPNEDIFYLVAFLPSAVPNSSGKNDLEYLLKQNQRVM 485

Query: 483 DFCEMAKLGAKQYLPHFDTQDGWRAHFGPRWDVFARRKLTYDPLSILTPGQRIFRK 538
           +FC  A L  KQYLPH++TQ  W++HFG RW+ FA+RK  YDPL+IL PGQRIF+K
Sbjct: 486 NFCAAANLNVKQYLPHYETQKEWKSHFGKRWETFAQRKQAYDPLAILAPGQRIFQK 541

BLAST of Spo06250.1 vs. ExPASy Swiss-Prot
Match: CKX6_ARATH (Cytokinin dehydrogenase 6 OS=Arabidopsis thaliana GN=CKX6 PE=2 SV=2)

HSP 1 Score: 691.8 bits (1784), Expect = 6.000e-198
Identity = 334/544 (61.40%), Postives = 425/544 (78.12%), Query Frame = 1

		  

Query: 2   LSCKFFRQSAEMLLKVFLVLLLNLAEHNINHCSYLPHATPDFTNLSSLELVSSLKKLNLN 61
           L     R+   ++++ F +LLL+     +  C         F++      +SSLK L L 
Sbjct: 4   LHASLLRKRTMLIVRSFTILLLSCIAFKLACC---------FSSS-----ISSLKALPLV 63

Query: 62  GNFDFENTRRVANDFGNRYHFLPMAVLLPKTVSDISTTIKHVINMGHNSELTVTARGHGH 121
           G+ +FE+    + DFGNRY  +P+AVL PK+VSDI++TI+H+  MG +S+LTV ARG GH
Sbjct: 64  GHLEFEHVHHASKDFGNRYQLIPLAVLHPKSVSDIASTIRHIWMMGTHSQLTVAARGRGH 123

Query: 122 SLQGQSQSHGGIVISMESLEEPKMHVHTGEQP--YVDVSGGELWINILHETLKQGLSPKS 181
           SLQGQ+Q+  GIVI MESL   K+ V++ + P  YVDVSGGELWINILHETLK GL+PKS
Sbjct: 124 SLQGQAQTRHGIVIHMESLHPQKLQVYSVDSPAPYVDVSGGELWINILHETLKYGLAPKS 183

Query: 182 WTDYLYLTVGGTLSNAGISGQAFRYGPQINNVHRLEIVTGKGDILNCSAKENADLFYGVL 241
           WTDYL+LTVGGTLSNAGISGQAFR+GPQI+NVH+LEIVTGKG+ILNC+ ++N+DLF GVL
Sbjct: 184 WTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGEILNCTKRQNSDLFNGVL 243

Query: 242 GGLGQFGIITRARISLEPAPRSVKWIRVLYSDFNLFTKDQEQLISSD-NSFDYIEGFVII 301
           GGLGQFGIITRARI+LEPAP  VKWIRVLY DF  F KDQEQLIS+  + FDYIEGFVII
Sbjct: 244 GGLGQFGIITRARIALEPAPTMVKWIRVLYLDFAAFAKDQEQLISAQGHKFDYIEGFVII 303

Query: 302 NRTGLINNWRSTFSPREPLQASQFSSEGKTLYCLEMAKYFNPEDGNIINQETEHLLSKLN 361
           NRTGL+N+WR +F+  EPL+ASQF  +G+TLYCLE+AKY   ++ ++INQE +  LS+L+
Sbjct: 304 NRTGLLNSWRLSFTAEEPLEASQFKFDGRTLYCLELAKYLKQDNKDVINQEVKETLSELS 363

Query: 362 YISHTLFQSEVSYVNFLNRVHLSEIKLREKGLWDVPHPWLNLLVPKSKIYSFAEEVFGHI 421
           Y++ TLF +EV+Y  FL+RVH+SE+KLR KG W+VPHPWLNLLVP+SKI  FA  VFG+I
Sbjct: 364 YVTSTLFTTEVAYEAFLDRVHVSEVKLRSKGQWEVPHPWLNLLVPRSKINEFARGVFGNI 423

Query: 422 LTDTSNGPILIYPVNQSRWNHKTSMVTPSEDVFYLVAFLTSAMPSSSGSDGLENILTKNK 481
           LTDTSNGP+++YPVN+S+W+++TS VTP E+VFYLVA LTSA P S+G DG+E IL +N+
Sbjct: 424 LTDTSNGPVIVYPVNKSKWDNQTSAVTPEEEVFYLVAILTSASPGSAGKDGVEEILRRNR 483

Query: 482 RILDFCEMAKLGAKQYLPHFDTQDGWRAHFGPRWDVFARRKLTYDPLSILTPGQRIFRKG 541
           RIL+F E A +G KQYLPH+ T++ WR+HFG +W  F RRK  YDPL+IL PG RIF+K 
Sbjct: 484 RILEFSEEAGIGLKQYLPHYTTREEWRSHFGDKWGEFVRRKSRYDPLAILAPGHRIFQKA 533

Query: 542 RAIS 543
            + S
Sbjct: 544 VSYS 533

BLAST of Spo06250.1 vs. ExPASy Swiss-Prot
Match: CKX9_ORYSJ (Cytokinin dehydrogenase 9 OS=Oryza sativa subsp. japonica GN=CKX9 PE=2 SV=1)

HSP 1 Score: 662.9 bits (1709), Expect = 3.000e-189
Identity = 300/491 (61.10%), Postives = 398/491 (81.06%), Query Frame = 1

		  

Query: 50  ELVSSLKKLNLNGNFDFENTRRVANDFGNRYHFLPMAVLLPKTVSDISTTIKHVINMGHN 109
           ++ SSL++L L+G+F F +    A DFGN   F P+AVL P +V+DI+TTI+HV  MG +
Sbjct: 29  DVPSSLEELTLDGHFSFHDVSAAAQDFGNLSSFPPVAVLHPGSVADIATTIRHVFLMGEH 88

Query: 110 SELTVTARGHGHSLQGQSQSHGGIVISMESLEEPKMHVHTGEQPYVDVSGGELWINILHE 169
           S LTV ARGHGHSL GQSQ+  GI+ISMESL+   M V+ G  PYVD SGGELWIN+LHE
Sbjct: 89  STLTVAARGHGHSLYGQSQAAEGIIISMESLQSNTMRVNPGVSPYVDASGGELWINVLHE 148

Query: 170 TLKQGLSPKSWTDYLYLTVGGTLSNAGISGQAFRYGPQINNVHRLEIVTGKGDILNCSAK 229
           TLK GL+PKSWTDYL+LTVGGTLSNAG+SGQ FR+GPQI+NV+ LEIVTG+GD++ CS +
Sbjct: 149 TLKYGLAPKSWTDYLHLTVGGTLSNAGVSGQTFRHGPQISNVNELEIVTGRGDVITCSPE 208

Query: 230 ENADLFYGVLGGLGQFGIITRARISLEPAPRSVKWIRVLYSDFNLFTKDQEQLISSDNSF 289
           +N+DLF+  LGGLGQFG+ITRARI LEPAP+ V+W+RVLY DF  FT+DQE LIS++ +F
Sbjct: 209 QNSDLFHAALGGLGQFGVITRARIPLEPAPKMVRWLRVLYLDFTSFTEDQEMLISAEKTF 268

Query: 290 DYIEGFVIINRTGLINNWRSTFSPREPLQASQFSSEGKTLYCLEMAKYFNPEDGNIINQE 349
           DYIEGFVIINRTG++NNWRS+F+P++P+++SQF S+GK L+CLEM K FNP++ +++ QE
Sbjct: 269 DYIEGFVIINRTGILNNWRSSFNPQDPVRSSQFESDGKVLFCLEMTKNFNPDEADVMEQE 328

Query: 350 TEHLLSKLNYISHTLFQSEVSYVNFLNRVHLSEIKLREKGLWDVPHPWLNLLVPKSKIYS 409
              LLS+L Y+  +LF ++V+Y+ FL+RVH SE+KLR KG+W+VPHPWLN+++P+S I+ 
Sbjct: 329 VNTLLSQLRYMPSSLFHTDVTYIEFLDRVHSSEMKLRAKGMWEVPHPWLNIIIPRSMIHK 388

Query: 410 FAEEVFGHILTDTSNGPILIYPVNQSRWNHKTSMVTPSEDVFYLVAFLTSAMPSSSGSDG 469
           FA+EVFG IL D++NGPIL+YPVN+SRW+++TS+V P E+VFYLVAFL+SA+    G   
Sbjct: 389 FAKEVFGKILKDSNNGPILLYPVNKSRWDNRTSVVIPDEEVFYLVAFLSSAL----GPHN 448

Query: 470 LENILTKNKRILDFCEMAKLGAKQYLPHFDTQDGWRAHFGPRWDVFARRKLTYDPLSILT 529
           +++ L  N RI++F + A +G KQYLP++ T+  W++HFG RWD F +RK  YDPL+IL 
Sbjct: 449 IKHTLDLNYRIIEFSDKAGIGVKQYLPNYTTEQEWQSHFGARWDTFQQRKKAYDPLAILA 508

Query: 530 PGQRIFRKGRA 541
           PGQRIF+K  A
Sbjct: 509 PGQRIFQKASA 515

BLAST of Spo06250.1 vs. ExPASy Swiss-Prot
Match: CKX4_ORYSJ (Cytokinin dehydrogenase 4 OS=Oryza sativa subsp. japonica GN=CKX4 PE=2 SV=1)

HSP 1 Score: 659.8 bits (1701), Expect = 2.500e-188
Identity = 302/491 (61.51%), Postives = 395/491 (80.45%), Query Frame = 1

		  

Query: 50  ELVSSLKKLNLNGNFDFENTRRVANDFGNRYHFLPMAVLLPKTVSDISTTIKHVINMGHN 109
           ++V+SL  L L+G+F F++    A DFGNR   LP AVL P +VSD++ T++ V  +G +
Sbjct: 33  DVVASLGALRLDGHFSFDDAHAAARDFGNRCSLLPAAVLHPGSVSDVAATVRRVFQLGRS 92

Query: 110 SELTVTARGHGHSLQGQSQSHGGIVISMESLEEPK---MHVHTGEQPYVDVSGGELWINI 169
           S LTV ARGHGHSL GQSQ+ GGIV+ MESL       + VH G  P+VD  GGELWIN+
Sbjct: 93  SPLTVAARGHGHSLLGQSQAAGGIVVKMESLAAAAARAVRVHGGASPHVDAPGGELWINV 152

Query: 170 LHETLKQGLSPKSWTDYLYLTVGGTLSNAGISGQAFRYGPQINNVHRLEIVTGKGDILNC 229
           LHETLK GL+P+SWTDYL+LTVGGTLSNAG+SGQAFR+GPQ++NV++LEIVTG+G+++ C
Sbjct: 153 LHETLKHGLAPRSWTDYLHLTVGGTLSNAGVSGQAFRHGPQVSNVNQLEIVTGRGEVVTC 212

Query: 230 SAKENADLFYGVLGGLGQFGIITRARISLEPAPRSVKWIRVLYSDFNLFTKDQEQLISSD 289
           S + N+DLFY  LGGLGQFGIITRARI+LEPAP+ V+WIRVLYSDF  FT+DQE+LI+S+
Sbjct: 213 SHEVNSDLFYAALGGLGQFGIITRARIALEPAPKMVRWIRVLYSDFETFTEDQEKLIASE 272

Query: 290 NSFDYIEGFVIINRTGLINNWRSTFSPREPLQASQFSSEGKTLYCLEMAKYFNPEDGNII 349
            +FDYIEGFVIINRTG++NNWR++F P++P+QASQF S+G+ LYCLE+   FN ++ +I+
Sbjct: 273 KTFDYIEGFVIINRTGILNNWRTSFKPQDPVQASQFQSDGRVLYCLELTMNFNHDEADIM 332

Query: 350 NQETEHLLSKLNYISHTLFQSEVSYVNFLNRVHLSEIKLREKGLWDVPHPWLNLLVPKSK 409
            QE   LLS+L YIS TLF ++V+Y+ FL+RVH SE+KLR +GLW+VPHPWLNLL+P+S 
Sbjct: 333 EQEVGALLSRLRYISSTLFYTDVTYLEFLDRVHTSELKLRAQGLWEVPHPWLNLLIPRST 392

Query: 410 IYSFAEEVFGHILTDTSNGPILIYPVNQSRWNHKTSMVTPSEDVFYLVAFLTSAMPSSSG 469
           ++ FA+EVFG IL D++NGPIL+YPVN+++W+++TS+V P E++FYLV FL+SA PSSSG
Sbjct: 393 VHKFAKEVFGKILKDSNNGPILLYPVNRTKWDNRTSVVIPDEEIFYLVGFLSSA-PSSSG 452

Query: 470 SDGLENILTKNKRILDFCEMAKLGAKQYLPHFDTQDGWRAHFGPRWDVFARRKLTYDPLS 529
              +E+ +  N +I+DFCE   +G KQYL  + TQ  W+AHFG RW+ F RRK TYDPL+
Sbjct: 453 HGSVEHAMNLNNKIVDFCEKNGVGMKQYLAPYTTQKQWKAHFGARWETFERRKHTYDPLA 512

Query: 530 ILTPGQRIFRK 538
           IL PGQRIF K
Sbjct: 513 ILAPGQRIFPK 522

BLAST of Spo06250.1 vs. ExPASy Swiss-Prot
Match: CKX5_ARATH (Cytokinin dehydrogenase 5 OS=Arabidopsis thaliana GN=CKX5 PE=2 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 5.700e-148
Identity = 261/488 (53.48%), Postives = 349/488 (71.52%), Query Frame = 1

		  

Query: 58  LNLNGNFDFE--NTRRVANDFGN-RYHFLPMAVLLPKTVSDISTTIKHVINMGHNSELTV 117
           ++++G+F     +   V++DFG  +    P+AVL P +  D++  ++     G  +   V
Sbjct: 38  IDVDGHFTVHPSDLASVSSDFGMLKSPEEPLAVLHPSSAEDVARLVRTAY--GSATAFPV 97

Query: 118 TARGHGHSLQGQSQS-HGGIVISMES--LEEPKMHVHTGEQPYVDVSGGELWINILHETL 177
           +ARGHGHS+ GQ+ +   G+V+ M       PK  V   E  YVDV GGELW+++L +TL
Sbjct: 98  SARGHGHSINGQAAAGRNGVVVEMNHGVTGTPKPLVRPDEM-YVDVWGGELWVDVLKKTL 157

Query: 178 KQGLSPKSWTDYLYLTVGGTLSNAGISGQAFRYGPQINNVHRLEIVTGKGDILNCSAKEN 237
           + GL+PKSWTDYLYLTVGGTLSNAGISGQAF +GPQI+NV  L++VTGKG+++ CS +EN
Sbjct: 158 EHGLAPKSWTDYLYLTVGGTLSNAGISGQAFHHGPQISNVLELDVVTGKGEVMRCSEEEN 217

Query: 238 ADLFYGVLGGLGQFGIITRARISLEPAPRSVKWIRVLYSDFNLFTKDQEQLISSDNS--F 297
             LF+GVLGGLGQFGIITRARISLEPAP+ V+WIRVLYS F +FT+DQE LIS      F
Sbjct: 218 TRLFHGVLGGLGQFGIITRARISLEPAPQRVRWIRVLYSSFKVFTEDQEYLISMHGQLKF 277

Query: 298 DYIEGFVIINRTGLINNWRST-FSPREPLQASQFSSEGKTLYCLEMAKYFNPEDGNIINQ 357
           DY+EGFVI++  GL+NNWRS+ FSPR P++ S  SS G  LYCLE+ K ++  D  I++Q
Sbjct: 278 DYVEGFVIVDE-GLVNNWRSSFFSPRNPVKISSVSSNGSVLYCLEITKNYHDSDSEIVDQ 337

Query: 358 ETEHLLSKLNYISHTLFQSEVSYVNFLNRVHLSEIKLREKGLWDVPHPWLNLLVPKSKIY 417
           E E L+ KLN+I  ++F +++ YV+FL+RVH +E+KLR K LW+VPHPWLNL VPKS+I 
Sbjct: 338 EVEILMKKLNFIPTSVFTTDLQYVDFLDRVHKAELKLRSKNLWEVPHPWLNLFVPKSRIS 397

Query: 418 SFAEEVFGHILTDTSNGPILIYPVNQSRWNHKTSMVTPSEDVFYLVAFLTSAMPSSSGSD 477
            F + VF  IL + ++GPILIYP+N+ +W+ ++S VTP E+VFYLVA L SA+     + 
Sbjct: 398 DFDKGVFKGILGNKTSGPILIYPMNKDKWDERSSAVTPDEEVFYLVALLRSALTDGEETQ 457

Query: 478 GLENILTKNKRILDFCEMAKLGAKQYLPHFDTQDGWRAHFGPRWDVFARRKLTYDPLSIL 537
            LE +  +N+RIL+FCE AK+  KQYLPH  TQ+ W AHFG +WD F   K  +DP  IL
Sbjct: 458 KLEYLKDQNRRILEFCEQAKINVKQYLPHHATQEEWVAHFGDKWDRFRSLKAEFDPRHIL 517

BLAST of Spo06250.1 vs. TAIR (Arabidopsis)
Match: AT2G41510.1 (cytokinin oxidase/dehydrogenase 1)

HSP 1 Score: 726.9 bits (1875), Expect = 9.400e-210
Identity = 336/536 (62.69%), Postives = 429/536 (80.04%), Query Frame = 1

		  

Query: 3   SCKFFRQSAEMLLKVFLVLLLNLAEHNINHCSYLPHATP-DFTNLSSLELVSSLKKLNLN 62
           S +F RQ+ +  L +F++L+L+      N CS    +TP +  + +  ++ SSL  L+L 
Sbjct: 6   SLRFHRQNNKTFLGIFMILVLSCIPGRTNLCSNHSVSTPKELPSSNPSDIRSSLVSLDLE 65

Query: 63  GNFDFENTRRVANDFGNRYHFLPMAVLLPKTVSDISTTIKHVINMGHNSELTVTARGHGH 122
           G   F++   VA DFGNRY   P+A+L P++V DIS+ +KH++++G  S LTV ARGHGH
Sbjct: 66  GYISFDDVHNVAKDFGNRYQLPPLAILHPRSVFDISSMMKHIVHLGSTSNLTVAARGHGH 125

Query: 123 SLQGQSQSHGGIVISMESLEEPKMHVHTGEQPYVDVSGGELWINILHETLKQGLSPKSWT 182
           SLQGQ+ +H G+VI MESL  P + ++ G+QPYVDVSGGE+WINIL ETLK GLSPKSWT
Sbjct: 126 SLQGQALAHQGVVIKMESLRSPDIRIYKGKQPYVDVSGGEIWINILRETLKYGLSPKSWT 185

Query: 183 DYLYLTVGGTLSNAGISGQAFRYGPQINNVHRLEIVTGKGDILNCSAKENADLFYGVLGG 242
           DYL+LTVGGTLSNAGISGQAF++GPQINNV++LEIVTGKG+++ CS K N++LF+ VLGG
Sbjct: 186 DYLHLTVGGTLSNAGISGQAFKHGPQINNVYQLEIVTGKGEVVTCSEKRNSELFFSVLGG 245

Query: 243 LGQFGIITRARISLEPAPRSVKWIRVLYSDFNLFTKDQEQLISSDNSFDYIEGFVIINRT 302
           LGQFGIITRARISLEPAP  VKWIRVLYSDF+ F++DQE LIS + +FDY+EGFVIINRT
Sbjct: 246 LGQFGIITRARISLEPAPHMVKWIRVLYSDFSAFSRDQEYLISKEKTFDYVEGFVIINRT 305

Query: 303 GLINNWRSTFSPREPLQASQFSSEGKTLYCLEMAKYFNPEDGNIINQETEHLLSKLNYIS 362
            L+NNWRS+FSP +  QAS+F S+GKTLYCLE+ KYFNPE+ + ++QET  LLS+LNYI 
Sbjct: 306 DLLNNWRSSFSPNDSTQASRFKSDGKTLYCLEVVKYFNPEEASSMDQETGKLLSELNYIP 365

Query: 363 HTLFQSEVSYVNFLNRVHLSEIKLREKGLWDVPHPWLNLLVPKSKIYSFAEEVFGHILTD 422
            TLF SEV Y+ FL+RVH++E KLR KGLW+VPHPWLNLL+PKS IY FA EVF +ILT 
Sbjct: 366 STLFSSEVPYIEFLDRVHIAERKLRAKGLWEVPHPWLNLLIPKSSIYQFATEVFNNILTS 425

Query: 423 TSNGPILIYPVNQSRWNHKTSMVTPSEDVFYLVAFLTSAMPSSSGSDGLENILTKNKRIL 482
            +NGPILIYPVNQS+W   TS++TP+ED+FYLVAFL SA+P+SSG + LE +L +N+R++
Sbjct: 426 NNNGPILIYPVNQSKWKKHTSLITPNEDIFYLVAFLPSAVPNSSGKNDLEYLLKQNQRVM 485

Query: 483 DFCEMAKLGAKQYLPHFDTQDGWRAHFGPRWDVFARRKLTYDPLSILTPGQRIFRK 538
           +FC  A L  KQYLPH++TQ  W++HFG RW+ FA+RK  YDPL+IL PGQRIF+K
Sbjct: 486 NFCAAANLNVKQYLPHYETQKEWKSHFGKRWETFAQRKQAYDPLAILAPGQRIFQK 541

BLAST of Spo06250.1 vs. TAIR (Arabidopsis)
Match: AT3G63440.1 (cytokinin oxidase/dehydrogenase 6)

HSP 1 Score: 691.8 bits (1784), Expect = 3.400e-199
Identity = 334/544 (61.40%), Postives = 425/544 (78.12%), Query Frame = 1

		  

Query: 2   LSCKFFRQSAEMLLKVFLVLLLNLAEHNINHCSYLPHATPDFTNLSSLELVSSLKKLNLN 61
           L     R+   ++++ F +LLL+     +  C         F++      +SSLK L L 
Sbjct: 4   LHASLLRKRTMLIVRSFTILLLSCIAFKLACC---------FSSS-----ISSLKALPLV 63

Query: 62  GNFDFENTRRVANDFGNRYHFLPMAVLLPKTVSDISTTIKHVINMGHNSELTVTARGHGH 121
           G+ +FE+    + DFGNRY  +P+AVL PK+VSDI++TI+H+  MG +S+LTV ARG GH
Sbjct: 64  GHLEFEHVHHASKDFGNRYQLIPLAVLHPKSVSDIASTIRHIWMMGTHSQLTVAARGRGH 123

Query: 122 SLQGQSQSHGGIVISMESLEEPKMHVHTGEQP--YVDVSGGELWINILHETLKQGLSPKS 181
           SLQGQ+Q+  GIVI MESL   K+ V++ + P  YVDVSGGELWINILHETLK GL+PKS
Sbjct: 124 SLQGQAQTRHGIVIHMESLHPQKLQVYSVDSPAPYVDVSGGELWINILHETLKYGLAPKS 183

Query: 182 WTDYLYLTVGGTLSNAGISGQAFRYGPQINNVHRLEIVTGKGDILNCSAKENADLFYGVL 241
           WTDYL+LTVGGTLSNAGISGQAFR+GPQI+NVH+LEIVTGKG+ILNC+ ++N+DLF GVL
Sbjct: 184 WTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGEILNCTKRQNSDLFNGVL 243

Query: 242 GGLGQFGIITRARISLEPAPRSVKWIRVLYSDFNLFTKDQEQLISSD-NSFDYIEGFVII 301
           GGLGQFGIITRARI+LEPAP  VKWIRVLY DF  F KDQEQLIS+  + FDYIEGFVII
Sbjct: 244 GGLGQFGIITRARIALEPAPTMVKWIRVLYLDFAAFAKDQEQLISAQGHKFDYIEGFVII 303

Query: 302 NRTGLINNWRSTFSPREPLQASQFSSEGKTLYCLEMAKYFNPEDGNIINQETEHLLSKLN 361
           NRTGL+N+WR +F+  EPL+ASQF  +G+TLYCLE+AKY   ++ ++INQE +  LS+L+
Sbjct: 304 NRTGLLNSWRLSFTAEEPLEASQFKFDGRTLYCLELAKYLKQDNKDVINQEVKETLSELS 363

Query: 362 YISHTLFQSEVSYVNFLNRVHLSEIKLREKGLWDVPHPWLNLLVPKSKIYSFAEEVFGHI 421
           Y++ TLF +EV+Y  FL+RVH+SE+KLR KG W+VPHPWLNLLVP+SKI  FA  VFG+I
Sbjct: 364 YVTSTLFTTEVAYEAFLDRVHVSEVKLRSKGQWEVPHPWLNLLVPRSKINEFARGVFGNI 423

Query: 422 LTDTSNGPILIYPVNQSRWNHKTSMVTPSEDVFYLVAFLTSAMPSSSGSDGLENILTKNK 481
           LTDTSNGP+++YPVN+S+W+++TS VTP E+VFYLVA LTSA P S+G DG+E IL +N+
Sbjct: 424 LTDTSNGPVIVYPVNKSKWDNQTSAVTPEEEVFYLVAILTSASPGSAGKDGVEEILRRNR 483

Query: 482 RILDFCEMAKLGAKQYLPHFDTQDGWRAHFGPRWDVFARRKLTYDPLSILTPGQRIFRKG 541
           RIL+F E A +G KQYLPH+ T++ WR+HFG +W  F RRK  YDPL+IL PG RIF+K 
Sbjct: 484 RILEFSEEAGIGLKQYLPHYTTREEWRSHFGDKWGEFVRRKSRYDPLAILAPGHRIFQKA 533

Query: 542 RAIS 543
            + S
Sbjct: 544 VSYS 533

BLAST of Spo06250.1 vs. TAIR (Arabidopsis)
Match: AT1G75450.1 (cytokinin oxidase 5)

HSP 1 Score: 525.8 bits (1353), Expect = 3.200e-149
Identity = 261/488 (53.48%), Postives = 349/488 (71.52%), Query Frame = 1

		  

Query: 58  LNLNGNFDFE--NTRRVANDFGN-RYHFLPMAVLLPKTVSDISTTIKHVINMGHNSELTV 117
           ++++G+F     +   V++DFG  +    P+AVL P +  D++  ++     G  +   V
Sbjct: 38  IDVDGHFTVHPSDLASVSSDFGMLKSPEEPLAVLHPSSAEDVARLVRTAY--GSATAFPV 97

Query: 118 TARGHGHSLQGQSQS-HGGIVISMES--LEEPKMHVHTGEQPYVDVSGGELWINILHETL 177
           +ARGHGHS+ GQ+ +   G+V+ M       PK  V   E  YVDV GGELW+++L +TL
Sbjct: 98  SARGHGHSINGQAAAGRNGVVVEMNHGVTGTPKPLVRPDEM-YVDVWGGELWVDVLKKTL 157

Query: 178 KQGLSPKSWTDYLYLTVGGTLSNAGISGQAFRYGPQINNVHRLEIVTGKGDILNCSAKEN 237
           + GL+PKSWTDYLYLTVGGTLSNAGISGQAF +GPQI+NV  L++VTGKG+++ CS +EN
Sbjct: 158 EHGLAPKSWTDYLYLTVGGTLSNAGISGQAFHHGPQISNVLELDVVTGKGEVMRCSEEEN 217

Query: 238 ADLFYGVLGGLGQFGIITRARISLEPAPRSVKWIRVLYSDFNLFTKDQEQLISSDNS--F 297
             LF+GVLGGLGQFGIITRARISLEPAP+ V+WIRVLYS F +FT+DQE LIS      F
Sbjct: 218 TRLFHGVLGGLGQFGIITRARISLEPAPQRVRWIRVLYSSFKVFTEDQEYLISMHGQLKF 277

Query: 298 DYIEGFVIINRTGLINNWRST-FSPREPLQASQFSSEGKTLYCLEMAKYFNPEDGNIINQ 357
           DY+EGFVI++  GL+NNWRS+ FSPR P++ S  SS G  LYCLE+ K ++  D  I++Q
Sbjct: 278 DYVEGFVIVDE-GLVNNWRSSFFSPRNPVKISSVSSNGSVLYCLEITKNYHDSDSEIVDQ 337

Query: 358 ETEHLLSKLNYISHTLFQSEVSYVNFLNRVHLSEIKLREKGLWDVPHPWLNLLVPKSKIY 417
           E E L+ KLN+I  ++F +++ YV+FL+RVH +E+KLR K LW+VPHPWLNL VPKS+I 
Sbjct: 338 EVEILMKKLNFIPTSVFTTDLQYVDFLDRVHKAELKLRSKNLWEVPHPWLNLFVPKSRIS 397

Query: 418 SFAEEVFGHILTDTSNGPILIYPVNQSRWNHKTSMVTPSEDVFYLVAFLTSAMPSSSGSD 477
            F + VF  IL + ++GPILIYP+N+ +W+ ++S VTP E+VFYLVA L SA+     + 
Sbjct: 398 DFDKGVFKGILGNKTSGPILIYPMNKDKWDERSSAVTPDEEVFYLVALLRSALTDGEETQ 457

Query: 478 GLENILTKNKRILDFCEMAKLGAKQYLPHFDTQDGWRAHFGPRWDVFARRKLTYDPLSIL 537
            LE +  +N+RIL+FCE AK+  KQYLPH  TQ+ W AHFG +WD F   K  +DP  IL
Sbjct: 458 KLEYLKDQNRRILEFCEQAKINVKQYLPHHATQEEWVAHFGDKWDRFRSLKAEFDPRHIL 517

BLAST of Spo06250.1 vs. TAIR (Arabidopsis)
Match: AT5G56970.1 (cytokinin oxidase 3)

HSP 1 Score: 471.9 bits (1213), Expect = 5.500e-133
Identity = 225/469 (47.97%), Postives = 311/469 (66.31%), Query Frame = 1

		  

Query: 73  ANDFGNRYHFLPMAVLLPKTVSDISTTIKHVINMGHNSELTVTARGHGHSLQGQSQSHGG 132
           A DFG+     P AVL+P +V DI+  IK  ++        + ARGHGHS +GQ+ +  G
Sbjct: 59  ATDFGHVTKIFPSAVLIPSSVEDITDLIK--LSFDSQLSFPLAARGHGHSHRGQASAKDG 118

Query: 133 IVISMESLEEPKMHVHTGEQP-YVDVSGGELWINILHETLKQGLSPKSWTDYLYLTVGGT 192
           +V++M S+      +       YVDV    LWI +L++TL+ GL+P SWTDYLYLTVGGT
Sbjct: 119 VVVNMRSMVNRDRGIKVSRTCLYVDVDAAWLWIEVLNKTLELGLTPVSWTDYLYLTVGGT 178

Query: 193 LSNAGISGQAFRYGPQINNVHRLEIVTGKGDILNCSAKENADLFYGVLGGLGQFGIITRA 252
           LSN GISGQ FRYGPQI NV  ++++TGKG+I  CS   N+DLF+ VLGGLGQFGIITRA
Sbjct: 179 LSNGGISGQTFRYGPQITNVLEMDVITGKGEIATCSKDMNSDLFFAVLGGLGQFGIITRA 238

Query: 253 RISLEPAPRSVKWIRVLYSDFNLFTKDQEQLISSDNSFDYIEGFVIINRTGLINNWRST- 312
           RI LE AP+  KW+R LY DF+ FT+DQE++IS  +  D++EG ++++  G  +NWRST 
Sbjct: 239 RIKLEVAPKRAKWLRFLYIDFSEFTRDQERVISKTDGVDFLEGSIMVDH-GPPDNWRSTY 298

Query: 313 FSPREPLQASQFSSEGKTLYCLEMAKYFNPEDGNIINQETEHLLSKLNYISHTLFQSEVS 372
           + P + L+ +      + +YCLE+ KY++      +N+E E L   LN++   +++ +V+
Sbjct: 299 YPPSDHLRIASMVKRHRVIYCLEVVKYYDETSQYTVNEEMEELSDSLNHVRGFMYEKDVT 358

Query: 373 YVNFLNRVHLSEIKLREKGLWDVPHPWLNLLVPKSKIYSFAEEVFGHIL--TDTSNGPIL 432
           Y++FLNRV   E+ L+ KG WDVPHPWLNL VPK++I  F + VF  I+   + ++GP+L
Sbjct: 359 YMDFLNRVRTGELNLKSKGQWDVPHPWLNLFVPKTQISKFDDGVFKGIILRNNITSGPVL 418

Query: 433 IYPVNQSRWNHKTSMVTPSEDVFYLVAFLTSAMPSSSGSDGLENILTKNKRILDFCEMAK 492
           +YP+N+++WN + S   P EDVFY V FL SA     G D  E    +N  IL FCE A 
Sbjct: 419 VYPMNRNKWNDRMSAAIPEEDVFYAVGFLRSA-----GFDNWEAFDQENMEILKFCEDAN 478

Query: 493 LGAKQYLPHFDTQDGWRAHFGPRWDVFARRKLTYDPLSILTPGQRIFRK 538
           +G  QYLP+  +Q+GW  HFGPRW++F  RK  YDP  IL+PGQ IF+K
Sbjct: 479 MGVIQYLPYHSSQEGWVRHFGPRWNIFVERKYKYDPKMILSPGQNIFQK 519

BLAST of Spo06250.1 vs. TAIR (Arabidopsis)
Match: AT5G21482.1 (cytokinin oxidase 7)

HSP 1 Score: 421.8 bits (1083), Expect = 6.500e-118
Identity = 212/474 (44.73%), Postives = 302/474 (63.71%), Query Frame = 1

		  

Query: 75  DFGNRYHFLPMAVLLPKTVSDISTTIKHVINMGHNSELTVTARGHGHSLQGQSQSHGGIV 134
           DFG      P+AV+ P    DI+  +K  +    + +LTV ARG+GHS+ GQ+ + GG+V
Sbjct: 53  DFGGMNCVKPLAVVRPVGPEDIAGAVKAALR---SDKLTVAARGNGHSINGQAMAEGGLV 112

Query: 135 ISMESLEEPKMHVHT----GEQPYVDVSGGELWINILHETLKQ-GLSPKSWTDYLYLTVG 194
           + M +  E    V          +VDVSGG LW ++L   + + GL+P+SWTDYL LTVG
Sbjct: 113 VDMSTTAENHFEVGYLSGGDATAFVDVSGGALWEDVLKRCVSEYGLAPRSWTDYLGLTVG 172

Query: 195 GTLSNAGISGQAFRYGPQINNVHRLEIVTGKGDILNCSAKENADLFYGVLGGLGQFGIIT 254
           GTLSNAG+SGQAFRYGPQ +NV  L++VTG GD++ CS  EN++LF+ VLGGLGQFGIIT
Sbjct: 173 GTLSNAGVSGQAFRYGPQTSNVTELDVVTGNGDVVTCSEIENSELFFSVLGGLGQFGIIT 232

Query: 255 RARISLEPAPRSVKWIRVLYSDFNLFTKDQEQLISSDN--SFDYIEGFVIINRTGLINNW 314
           RAR+ L+PAP  V+WIRV+Y++F+ FT+D E L+S  N  SFDY+EGFV +N    +N W
Sbjct: 233 RARVLLQPAPDMVRWIRVVYTEFDEFTQDAEWLVSQKNESSFDYVEGFVFVNGADPVNGW 292

Query: 315 RST-FSPREPLQASQF-SSEGKTLYCLEMAKYFNPEDGN-IINQETEHLLSKLNYISHTL 374
            +    P      ++   S G  LYCLE+  ++   D N  I++  E L+ +L +     
Sbjct: 293 PTVPLHPDHEFDPTRLPQSCGSVLYCLELGLHYRDSDSNSTIDKRVERLIGRLRFNEGLR 352

Query: 375 FQSEVSYVNFLNRVHLSEIKLREKGLWDVPHPWLNLLVPKSKIYSFAEEVFGHILTDTSN 434
           F+ ++ YV+FL RV  SE   +E G W+ PHPWLNL V K  I  F   VF  ++ +  N
Sbjct: 353 FEVDLPYVDFLLRVKRSEEIAKENGTWETPHPWLNLFVSKRDIGDFNRTVFKELVKNGVN 412

Query: 435 GPILIYPVNQSRWNHKTSMVTPSE-DVFYLVAFLTSAMPSSSGSDGLENILTKNKRILDF 494
           GP+L+YP+ +SRW+ +TS+V P E ++FY+VA L    P +  S  +E ++ +N+ I+ +
Sbjct: 413 GPMLVYPLLRSRWDDRTSVVIPEEGEIFYIVALLRFVPPCAKVS-SVEKMVAQNQEIVHW 472

Query: 495 CEMAKLGAKQYLPHFDTQDGWRAHFGPRWDVFARRKLTYDPLSILTPGQRIFRK 538
           C    +  K YLPH+ +Q+ W  HFG RW  F  RK  +DP++IL+PGQ+IF +
Sbjct: 473 CVKNGIDYKLYLPHYKSQEEWIRHFGNRWSRFVDRKAMFDPMAILSPGQKIFNR 522

The following BLAST results are available for this feature:
BLAST of Spo06250.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|731324348|ref|XP_010672924.1|4.8e-28888.0PREDICTED: cytokinin dehydroge... [more]
gi|731439660|ref|XP_002284560.2|1.8e-23470.0PREDICTED: cytokinin dehydroge... [more]
gi|720076342|ref|XP_010279585.1|3.8e-22969.3PREDICTED: cytokinin dehydroge... [more]
gi|567856976|ref|XP_006421171.1|3.1e-22369.0hypothetical protein CICLE_v10... [more]
gi|568876876|ref|XP_006491496.1|4.1e-22369.2PREDICTED: cytokinin dehydroge... [more]
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BLAST of Spo06250.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0J8CSR7_BETVU3.3e-28888.0Uncharacterized protein OS=Bet... [more]
D7TSA7_VITVI1.2e-23470.0Putative uncharacterized prote... [more]
V4SBS5_9ROSI2.2e-22369.0Uncharacterized protein OS=Cit... [more]
B9H9J6_POPTR6.6e-22068.6Uncharacterized protein OS=Pop... [more]
A0A061FLK6_THECC2.5e-21966.8Cytokinin oxidase/dehydrogenas... [more]
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BLAST of Spo06250.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5
Match NameE-valueIdentityDescription
CKX1_ARATH1.7e-20862.6Cytokinin dehydrogenase 1 OS=A... [more]
CKX6_ARATH6.0e-19861.4Cytokinin dehydrogenase 6 OS=A... [more]
CKX9_ORYSJ3.0e-18961.1Cytokinin dehydrogenase 9 OS=O... [more]
CKX4_ORYSJ2.5e-18861.5Cytokinin dehydrogenase 4 OS=O... [more]
CKX5_ARATH5.7e-14853.4Cytokinin dehydrogenase 5 OS=A... [more]
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BLAST of Spo06250.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5
Match NameE-valueIdentityDescription
AT2G41510.19.4e-21062.6cytokinin oxidase/dehydrogenas... [more]
AT3G63440.13.4e-19961.4cytokinin oxidase/dehydrogenas... [more]
AT1G75450.13.2e-14953.4cytokinin oxidase 5[more]
AT5G56970.15.5e-13347.9cytokinin oxidase 3[more]
AT5G21482.16.5e-11844.7cytokinin oxidase 7[more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006093Oxygen oxidoreductase covalent FAD-binding sitePROSITEPS00862OX2_COVAL_FADcoord: 84..119
scor
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 85..227
score: 6.0
IPR015345Cytokinin dehydrogenase 1, FAD/cytokinin binding domainPFAMPF09265Cytokin-bindcoord: 259..535
score: 2.1E
IPR016164FAD-linked oxidase-like, C-terminalunknownSSF55103FAD-linked oxidases, C-terminal domaincoord: 259..535
score: 1.16
IPR016166FAD-binding, type 2PROFILEPS51387FAD_PCMHcoord: 80..258
score: 15
IPR016166FAD-binding, type 2unknownSSF56176FAD-binding/transporter-associated domain-likecoord: 64..257
score: 1.07
IPR016167FAD-binding, type 2, subdomain 1GENE3D3.30.43.10coord: 65..140
score: 2.
IPR016169CO dehydrogenase flavoprotein-like, FAD-binding, subdomain 2GENE3D3.30.465.10coord: 150..257
score: 1.0
IPR016170Vanillyl-alcohol oxidase/Cytokinin dehydrogenase C-terminal domainGENE3D3.40.462.10coord: 262..494
score: 2.3
NoneNo IPR availablePANTHERPTHR13878GULONOLACTONE OXIDASEcoord: 52..542
score:
NoneNo IPR availablePANTHERPTHR13878:SF53CYTOKININ DEHYDROGENASE 6coord: 52..542
score:

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006040 amino sugar metabolic process
biological_process GO:0030003 cellular cation homeostasis
biological_process GO:0006457 protein folding
biological_process GO:0046685 response to arsenic-containing substance
biological_process GO:0009408 response to heat
biological_process GO:0009644 response to high light intensity
biological_process GO:0042542 response to hydrogen peroxide
biological_process GO:0044036 cell wall macromolecule metabolic process
biological_process GO:0009904 chloroplast accumulation movement
biological_process GO:0007018 microtubule-based movement
biological_process GO:0070838 divalent metal ion transport
biological_process GO:0000160 phosphorelay signal transduction system
biological_process GO:0042128 nitrate assimilation
biological_process GO:0006857 oligopeptide transport
biological_process GO:0009753 response to jasmonic acid
biological_process GO:0009611 response to wounding
biological_process GO:0009690 cytokinin metabolic process
biological_process GO:0006810 transport
biological_process GO:0006535 cysteine biosynthetic process from serine
biological_process GO:0042967 obsolete acyl-carrier-protein biosynthetic process
biological_process GO:0009816 defense response to bacterium, incompatible interaction
biological_process GO:0007275 multicellular organism development
biological_process GO:0006468 protein phosphorylation
biological_process GO:0009639 response to red or far red light
biological_process GO:0050789 regulation of biological process
biological_process GO:0009791 post-embryonic development
biological_process GO:0008152 metabolic process
biological_process GO:0009987 cellular process
biological_process GO:0010089 xylem development
biological_process GO:0009823 cytokinin catabolic process
biological_process GO:0048507 meristem development
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005874 microtubule
cellular_component GO:0000785 chromatin
cellular_component GO:0005737 cytoplasm
cellular_component GO:0045298 tubulin complex
cellular_component GO:0005618 cell wall
cellular_component GO:0005871 kinesin complex
cellular_component GO:0005622 intracellular
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0005829 cytosol
cellular_component GO:0005773 vacuole
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0009001 serine O-acetyltransferase activity
molecular_function GO:0016740 transferase activity
molecular_function GO:0015293 symporter activity
molecular_function GO:0019139 cytokinin dehydrogenase activity
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0008762 UDP-N-acetylmuramate dehydrogenase activity
molecular_function GO:0005515 protein binding
molecular_function GO:0003777 microtubule motor activity
molecular_function GO:0008017 microtubule binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0051082 unfolded protein binding
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0003682 chromatin binding
RNA-Seq Expression