Spo06718 (gene) | SpinachBase

Overview


Name	Spo06718
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	ATP-dependent RNA helicase eIF4A isoform 3
Location	SpoScf_04309 : 9353 .. 9817 (+)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGATAGACTCCATAGAGACCTCTTCGTCTCCTTCGCAATCGCCTTCTTATACAATCGGGTACGTAATTGTACAATCCTCTAAATCTCCTCGTTTTTTTTTTACTTAATACGAAGTATTTTGTTTCCTTCCCCATTTTAATGGAGCTTCGCAATTCATCTTTCCTGCAGTCAGCCTCGCCACTTCTACCTCGCCGTCGATCGCCCCCCTTTCAAAATAGTACATATATATCTATCTCTCATAATTCTACATTTAGAAAGAAAACCTTAATTTTGAATTTTTCTGATTACTGAAAATTTTGGGATTATTTTGATATAATTTGCAGGAGACATTGATTGATTTGCTCGGAGTAGTAGGGCGAAGATCAGGACTTCCCATGGTAGTGTGTTGCAGTTCTCGCGATGAGCTTGACGCTGTTTGTTCTACCGTCTCTTCTCTTCCGTACATTTCGCTCTCCTCCTTG

mRNA sequence

ATGGCGATAGACTCCATAGAGACCTCTTCGTCTCCTTCGCAATCGCCTTCTTATACAATCGGGTACGAGACATTGATTGATTTGCTCGGAGTAGTAGGGCGAAGATCAGGACTTCCCATGGTAGTGTGTTGCAGTTCTCGCGATGAGCTTGACGCTGTTTGTTCTACCGTCTCTTCTCTTCCGTACATTTCGCTCTCCTCCTTG

Coding sequence (CDS)

ATGGCGATAGACTCCATAGAGACCTCTTCGTCTCCTTCGCAATCGCCTTCTTATACAATCGGGTACGAGACATTGATTGATTTGCTCGGAGTAGTAGGGCGAAGATCAGGACTTCCCATGGTAGTGTGTTGCAGTTCTCGCGATGAGCTTGACGCTGTTTGTTCTACCGTCTCTTCTCTTCCGTACATTTCGCTCTCCTCCTTG

Protein sequence

MAIDSIETSSSPSQSPSYTIGYETLIDLLGVVGRRSGLPMVVCCSSRDELDAVCSTVSSLPYISLSSL

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo06718.1	Spo06718.1	mRNA

Homology

[Close]

BLAST of Spo06718.1 vs. NCBI nr
Match: gi|902202941|gb|KNA14427.1| (hypothetical protein SOVF_107510, partial [Spinacia oleracea])

HSP 1 Score: 119.8 bits (299), Expect = 1.900e-24
Identity = 67/83 (80.72%), Postives = 67/83 (80.72%), Query Frame = 1


		  
Query: 1   MAIDSIETSSSPSQSPSYTIGY---------------ETLIDLLGVVGRRSGLPMVVCCS 60
           MAIDSIETSSSPSQSPSYTIG                ETLIDLLGVVGRRSGLPMVVCCS
Sbjct: 28  MAIDSIETSSSPSQSPSYTIGQPRHFYLAVDRPPFKIETLIDLLGVVGRRSGLPMVVCCS 87

Query: 61  SRDELDAVCSTVSSLPYISLSSL 69
           SRDELDAVCSTVSSLPYISLSSL
Sbjct: 88  SRDELDAVCSTVSSLPYISLSSL 110

[Close]

BLAST of Spo06718.1 vs. NCBI nr
Match: gi|731373347|ref|XP_010666624.1| (PREDICTED: uncharacterized protein LOC104883764 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 103.6 bits (257), Expect = 1.400e-19
Identity = 58/83 (69.88%), Postives = 61/83 (73.49%), Query Frame = 1


		  
Query: 1  MAIDSIETSSSPSQSPSYTIGY---------------ETLIDLLGVVGRRSGLPMVVCCS 60
          MA +  ET SSPSQSPSY +                 ETL+DLLGVVGRRSGLPMVVCCS
Sbjct: 1  MATEIGETPSSPSQSPSYPVSQLRHFYLAVDRPQFRVETLVDLLGVVGRRSGLPMVVCCS 60

Query: 61 SRDELDAVCSTVSSLPYISLSSL 69
          SRDELDAVCSTVSSLPYISLSSL
Sbjct: 61 SRDELDAVCSTVSSLPYISLSSL 83

[Close]

BLAST of Spo06718.1 vs. NCBI nr
Match: gi|590704716|ref|XP_007047236.1| (ATP-dependent RNA helicase eIF4A isoform 1 [Theobroma cacao])

HSP 1 Score: 91.7 bits (226), Expect = 5.500e-16
Identity = 52/85 (61.18%), Postives = 58/85 (68.24%), Query Frame = 1


		  
Query: 1  MAIDSIETSSSPSQSPSYTIG-----------------YETLIDLLGVVGRRSGLPMVVC 60
          MA+D+IE SS PSQSPS                      ETLIDLLGV GRR+ LP+VVC
Sbjct: 1  MALDAIEASSPPSQSPSNFCSSNQLRHFYVAVDRLHFKMETLIDLLGVAGRRTCLPIVVC 60

Query: 61 CSSRDELDAVCSTVSSLPYISLSSL 69
          CSSRDELDAVCS VS+LPY+SLSSL
Sbjct: 61 CSSRDELDAVCSAVSNLPYVSLSSL 85

[Close]

BLAST of Spo06718.1 vs. NCBI nr
Match: gi|590704719|ref|XP_007047237.1| (ATP-dependent RNA helicase eIF4A isoform 2 [Theobroma cacao])

HSP 1 Score: 91.7 bits (226), Expect = 5.500e-16
Identity = 52/85 (61.18%), Postives = 58/85 (68.24%), Query Frame = 1


		  
Query: 1  MAIDSIETSSSPSQSPSYTIG-----------------YETLIDLLGVVGRRSGLPMVVC 60
          MA+D+IE SS PSQSPS                      ETLIDLLGV GRR+ LP+VVC
Sbjct: 1  MALDAIEASSPPSQSPSNFCSSNQLRHFYVAVDRLHFKMETLIDLLGVAGRRTCLPIVVC 60

Query: 61 CSSRDELDAVCSTVSSLPYISLSSL 69
          CSSRDELDAVCS VS+LPY+SLSSL
Sbjct: 61 CSSRDELDAVCSAVSNLPYVSLSSL 85

[Close]

BLAST of Spo06718.1 vs. NCBI nr
Match: gi|590704723|ref|XP_007047238.1| (ATP-dependent RNA helicase eIF4A isoform 3 [Theobroma cacao])

HSP 1 Score: 91.7 bits (226), Expect = 5.500e-16
Identity = 52/85 (61.18%), Postives = 58/85 (68.24%), Query Frame = 1


		  
Query: 1  MAIDSIETSSSPSQSPSYTIG-----------------YETLIDLLGVVGRRSGLPMVVC 60
          MA+D+IE SS PSQSPS                      ETLIDLLGV GRR+ LP+VVC
Sbjct: 1  MALDAIEASSPPSQSPSNFCSSNQLRHFYVAVDRLHFKMETLIDLLGVAGRRTCLPIVVC 60

Query: 61 CSSRDELDAVCSTVSSLPYISLSSL 69
          CSSRDELDAVCS VS+LPY+SLSSL
Sbjct: 61 CSSRDELDAVCSAVSNLPYVSLSSL 85

[Close]

BLAST of Spo06718.1 vs. UniProtKB/TrEMBL
Match: A0A0K9R6I9_SPIOL (Uncharacterized protein (Fragment) OS=Spinacia oleracea GN=SOVF_107510 PE=4 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 1.300e-24
Identity = 67/83 (80.72%), Postives = 67/83 (80.72%), Query Frame = 1


		  
Query: 1   MAIDSIETSSSPSQSPSYTIGY---------------ETLIDLLGVVGRRSGLPMVVCCS 60
           MAIDSIETSSSPSQSPSYTIG                ETLIDLLGVVGRRSGLPMVVCCS
Sbjct: 28  MAIDSIETSSSPSQSPSYTIGQPRHFYLAVDRPPFKIETLIDLLGVVGRRSGLPMVVCCS 87

Query: 61  SRDELDAVCSTVSSLPYISLSSL 69
           SRDELDAVCSTVSSLPYISLSSL
Sbjct: 88  SRDELDAVCSTVSSLPYISLSSL 110

[Close]

BLAST of Spo06718.1 vs. UniProtKB/TrEMBL
Match: A0A0J8B7N1_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_003340 PE=4 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 9.700e-20
Identity = 58/83 (69.88%), Postives = 61/83 (73.49%), Query Frame = 1


		  
Query: 1  MAIDSIETSSSPSQSPSYTIGY---------------ETLIDLLGVVGRRSGLPMVVCCS 60
          MA +  ET SSPSQSPSY +                 ETL+DLLGVVGRRSGLPMVVCCS
Sbjct: 1  MATEIGETPSSPSQSPSYPVSQLRHFYLAVDRPQFRVETLVDLLGVVGRRSGLPMVVCCS 60

Query: 61 SRDELDAVCSTVSSLPYISLSSL 69
          SRDELDAVCSTVSSLPYISLSSL
Sbjct: 61 SRDELDAVCSTVSSLPYISLSSL 83

[Close]

BLAST of Spo06718.1 vs. UniProtKB/TrEMBL
Match: A0A061DGD9_THECC (ATP-dependent RNA helicase eIF4A isoform 3 OS=Theobroma cacao GN=TCM_000603 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 3.800e-16
Identity = 52/85 (61.18%), Postives = 58/85 (68.24%), Query Frame = 1


		  
Query: 1  MAIDSIETSSSPSQSPSYTIG-----------------YETLIDLLGVVGRRSGLPMVVC 60
          MA+D+IE SS PSQSPS                      ETLIDLLGV GRR+ LP+VVC
Sbjct: 1  MALDAIEASSPPSQSPSNFCSSNQLRHFYVAVDRLHFKMETLIDLLGVAGRRTCLPIVVC 60

Query: 61 CSSRDELDAVCSTVSSLPYISLSSL 69
          CSSRDELDAVCS VS+LPY+SLSSL
Sbjct: 61 CSSRDELDAVCSAVSNLPYVSLSSL 85

[Close]

BLAST of Spo06718.1 vs. UniProtKB/TrEMBL
Match: A0A061DHV4_THECC (ATP-dependent RNA helicase eIF4A isoform 4 OS=Theobroma cacao GN=TCM_000603 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 3.800e-16
Identity = 52/85 (61.18%), Postives = 58/85 (68.24%), Query Frame = 1


		  
Query: 1  MAIDSIETSSSPSQSPSYTIG-----------------YETLIDLLGVVGRRSGLPMVVC 60
          MA+D+IE SS PSQSPS                      ETLIDLLGV GRR+ LP+VVC
Sbjct: 1  MALDAIEASSPPSQSPSNFCSSNQLRHFYVAVDRLHFKMETLIDLLGVAGRRTCLPIVVC 60

Query: 61 CSSRDELDAVCSTVSSLPYISLSSL 69
          CSSRDELDAVCS VS+LPY+SLSSL
Sbjct: 61 CSSRDELDAVCSAVSNLPYVSLSSL 85

[Close]

BLAST of Spo06718.1 vs. UniProtKB/TrEMBL
Match: A0A061DG91_THECC (ATP-dependent RNA helicase eIF4A isoform 2 OS=Theobroma cacao GN=TCM_000603 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 3.800e-16
Identity = 52/85 (61.18%), Postives = 58/85 (68.24%), Query Frame = 1


		  
Query: 1  MAIDSIETSSSPSQSPSYTIG-----------------YETLIDLLGVVGRRSGLPMVVC 60
          MA+D+IE SS PSQSPS                      ETLIDLLGV GRR+ LP+VVC
Sbjct: 1  MALDAIEASSPPSQSPSNFCSSNQLRHFYVAVDRLHFKMETLIDLLGVAGRRTCLPIVVC 60

Query: 61 CSSRDELDAVCSTVSSLPYISLSSL 69
          CSSRDELDAVCS VS+LPY+SLSSL
Sbjct: 61 CSSRDELDAVCSAVSNLPYVSLSSL 85

[Close]

BLAST of Spo06718.1 vs. TAIR (Arabidopsis)
Match: AT4G37020.2 (FUNCTIONS IN: molecular_function unknown)

HSP 1 Score: 79.3 bits (194), Expect = 9.900e-16
Identity = 43/80 (53.75%), Postives = 56/80 (70.00%), Query Frame = 1


		  
Query: 3  IDSIETSSSPSQSPSYT--------------IGYETLIDLLGVVGRRSGLPMVVCCSSRD 62
          +DS+E  S P QSPS +                 ET+++LLGV+GRR  LP+VVCCSSRD
Sbjct: 1  MDSMEAPSLPFQSPSRSSQQLHFYLAVDRPQFKMETVVELLGVLGRRPWLPIVVCCSSRD 60

Query: 63 ELDAVCSTVSSLPYISLSSL 69
          ELDAVCS++S+LPYISL++L
Sbjct: 61 ELDAVCSSLSTLPYISLAAL 80

The following BLAST results are available for this feature:

BLAST of Spo06718.1 vs. NCBI nr
BLAST of Spo06718.1 vs. UniProtKB/TrEMBL
BLAST of Spo06718.1 vs. ExPASy Swiss-Prot
BLAST of Spo06718.1 vs. TAIR (Arabidopsis)

BLAST of Spo06718.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902202941\|gb\|KNA14427.1\|	1.9e-24	80.7	hypothetical protein SOVF_1075...	[more]
gi\|731373347\|ref\|XP_010666624.1\|	1.4e-19	69.8	PREDICTED: uncharacterized pro...	[more]
gi\|590704716\|ref\|XP_007047236.1\|	5.5e-16	61.1	ATP-dependent RNA helicase eIF...	[more]
gi\|590704719\|ref\|XP_007047237.1\|	5.5e-16	61.1	ATP-dependent RNA helicase eIF...	[more]
gi\|590704723\|ref\|XP_007047238.1\|	5.5e-16	61.1	ATP-dependent RNA helicase eIF...	[more]

BLAST of Spo06718.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9R6I9_SPIOL	1.3e-24	80.7	Uncharacterized protein (Fragm...	[more]
A0A0J8B7N1_BETVU	9.7e-20	69.8	Uncharacterized protein OS=Bet...	[more]
A0A061DGD9_THECC	3.8e-16	61.1	ATP-dependent RNA helicase eIF...	[more]
A0A061DHV4_THECC	3.8e-16	61.1	ATP-dependent RNA helicase eIF...	[more]
A0A061DG91_THECC	3.8e-16	61.1	ATP-dependent RNA helicase eIF...	[more]

BLAST of Spo06718.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Spo06718.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1


Match Name	E-value	Identity	Description
AT4G37020.2	9.9e-16	53.7	FUNCTIONS IN: molecular_functi...	[more]

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
biological_process	GO:0009693	ethylene biosynthetic process
biological_process	GO:0006260	DNA replication
biological_process	GO:0051567	histone H3-K9 methylation
biological_process	GO:0009086	methionine biosynthetic process
biological_process	GO:0045893	positive regulation of transcription, DNA-templated
biological_process	GO:0043687	post-translational protein modification
biological_process	GO:0000394	RNA splicing, via endonucleolytic cleavage and ligation
biological_process	GO:0010050	vegetative phase change
biological_process	GO:0009616	virus induced gene silencing
biological_process	GO:0006121	mitochondrial electron transport, succinate to ubiquinone
biological_process	GO:0006099	tricarboxylic acid cycle
biological_process	GO:0019447	D-cysteine catabolic process
biological_process	GO:0006094	gluconeogenesis
biological_process	GO:0008283	cell proliferation
biological_process	GO:0019761	glucosinolate biosynthetic process
biological_process	GO:0006096	glycolytic process
biological_process	GO:0042744	hydrogen peroxide catabolic process
biological_process	GO:0019288	isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
biological_process	GO:0032880	regulation of protein localization
biological_process	GO:0009651	response to salt stress
biological_process	GO:0019521	D-gluconate metabolic process
biological_process	GO:0006550	isoleucine catabolic process
biological_process	GO:0006552	leucine catabolic process
biological_process	GO:0006098	pentose-phosphate shunt
biological_process	GO:0006574	valine catabolic process
biological_process	GO:0006306	DNA methylation
biological_process	GO:0033528	S-methylmethionine cycle
biological_process	GO:0060560	developmental growth involved in morphogenesis
biological_process	GO:0016049	cell growth
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0016558	protein import into peroxisome matrix
biological_process	GO:0042967	obsolete acyl-carrier-protein biosynthetic process
biological_process	GO:0016573	histone acetylation
biological_process	GO:0048869	cellular developmental process
biological_process	GO:0016043	cellular component organization
biological_process	GO:0006635	fatty acid beta-oxidation
biological_process	GO:0009058	biosynthetic process
biological_process	GO:0000023	maltose metabolic process
biological_process	GO:0006397	mRNA processing
biological_process	GO:0019252	starch biosynthetic process
biological_process	GO:0010015	root morphogenesis
biological_process	GO:0010817	regulation of hormone levels
biological_process	GO:0044238	primary metabolic process
biological_process	GO:1901576	organic substance biosynthetic process
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005743	mitochondrial inner membrane
cellular_component	GO:0045281	succinate dehydrogenase complex
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0080008	Cul4-RING E3 ubiquitin ligase complex
cellular_component	GO:0000123	histone acetyltransferase complex
cellular_component	GO:0005778	peroxisomal membrane
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0008660	1-aminocyclopropane-1-carboxylate deaminase activity
molecular_function	GO:0050897	cobalt ion binding
molecular_function	GO:0019148	D-cysteine desulfhydrase activity
molecular_function	GO:0005515	protein binding
molecular_function	GO:0008442	3-hydroxyisobutyrate dehydrogenase activity
molecular_function	GO:0051287	NAD binding
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0008177	succinate dehydrogenase (ubiquinone) activity
molecular_function	GO:0009055	electron transfer activity
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0051538	3 iron, 4 sulfur cluster binding
molecular_function	GO:0051537	2 iron, 2 sulfur cluster binding
molecular_function	GO:0008898	S-adenosylmethionine-homocysteine S-methyltransferase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004402	histone acetyltransferase activity
molecular_function	GO:0004386	helicase activity
molecular_function	GO:0003729	mRNA binding
molecular_function	GO:0000166	nucleotide binding
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0004616	phosphogluconate dehydrogenase (decarboxylating) activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo18143	0.81	Barchart \| Table
Spo11304	0.78	Barchart \| Table
Spo01066	0.78	Barchart \| Table
Spo04970	0.77	Barchart \| Table
Spo17466	0.77	Barchart \| Table
Spo24867	0.77	Barchart \| Table
Spo06714	0.77	Barchart \| Table
Spo23067	0.77	Barchart \| Table
Spo26313	0.76	Barchart \| Table
Spo11056	0.76	Barchart \| Table
Spo24203	0.76	Barchart \| Table
Spo10146	0.75	Barchart \| Table
Spo27236	0.75	Barchart \| Table
Spo15984	0.75	Barchart \| Table
Spo11682	0.74	Barchart \| Table
Spo01039	0.74	Barchart \| Table
Spo08682	0.73	Barchart \| Table
Spo24213	0.73	Barchart \| Table
Spo22586	0.72	Barchart \| Table
Spo17068	0.72	Barchart \| Table
Spo07446	0.71	Barchart \| Table
Spo16893	0.71	Barchart \| Table
Spo23964	0.71	Barchart \| Table
Spo14378	0.70	Barchart \| Table
Spo27227	0.70	Barchart \| Table
Spo03213	0.70	Barchart \| Table
Spo12796	0.69	Barchart \| Table
Spo04789	0.69	Barchart \| Table
Spo11336	0.69	Barchart \| Table
Spo16392	0.69	Barchart \| Table
Spo21412	0.69	Barchart \| Table
Spo22109	0.69	Barchart \| Table
Spo04959	0.68	Barchart \| Table
Spo02346	0.68	Barchart \| Table
Spo15092	0.68	Barchart \| Table
Spo26206	0.68	Barchart \| Table
Spo01279	0.67	Barchart \| Table
Spo08497	0.67	Barchart \| Table
Spo26205	0.67	Barchart \| Table
Spo01500	0.67	Barchart \| Table
Spo01754	0.66	Barchart \| Table
Spo04387	0.66	Barchart \| Table
Spo10817	0.66	Barchart \| Table
Spo05415	0.65	Barchart \| Table
Spo27285	0.65	Barchart \| Table