Spo07973 (gene) | SpinachBase

Overview


Name	Spo07973
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	(Thiamin pyrophosphokinase, putative) (2.7.6.2)
Location	Super_scaffold_124 : 469863 .. 476247 (-)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTCGGTATGACCAAATTTATCAGAGACATAAAACAGAAACAGGGCTCACAAAAATCGACGAGTTAAGAAAAACGAAATACCAAACTCACCAATTCCGCCATAATTGGAAGAATTCCATGGTTTATTTAAACCAAACACCTCTCCTTCAAACTCCATTGACAAACATCAAGCTAATCTCCCAACTCAACAACAAATCCCAACAAATAAATTCCACTTTGAACCCTGATTATTACAGTAATAATATGGAAGCAATGCAACACTCTTCGTCTTTTCTTCTTCCTTCAATTCCTATTGAACAAGGTGCTAATTCGCTAAATTACGCCTTAGTTATTCTTAACCAACGCCTACCTCGTTTTGCCCCTCTTCTATGGGAGCACGGTAAATTTTCTCTCTCCTATTTTTATTGGGTTTTTGTTTTTCCTTTTTTGGTAATTTTTGGCGGTTAGTTTTTGATTTGGGTGTGTGTATTTTTTGCGTAGAACAGCAAAATTGAGAGTTTGCGCAGATGGAGGAGGGAATCGTTTGTTCGATGAAATTCCGCAGTTTTTTCCTGAACAAAATCCCATTGATGTTCGCAACAGGTTTTTTTTTTTTTTTTTAAATAAAAAAAAATTGTTATTTTATGATCATGATATATTTCAATGTGAACTTTGTGTAATTATATTGGGAATCCTGTGTTTTTTCTTCTTGATTTTAGGTGTTATTAATTTCTTTGACTTTTTTTATTAAAGAAAAAAATTAAATGGTCTAATATGTTGGGTTGAGCTTGTTGATTGTTTGTTGTAGTTATGGAAATGTTGGGTTGAGCTTGTTGATTGTTTGTTGTATTTATGGAAATGTTGGGTTGAACTTGTTGATTGCTTGTAGTTATTGAAATGTTGGCTTGAATAATGCGCAAAAGTGGCACTTTGTATCATCTTGATAAAAACAAATGCAAATTTTTAGGTGAATTGCTGTAGCTAATTAGCACCCAACATTGAAATGGTTATTATTTTTAGGTGGGTTGTGGTTTTATTTATGCTTGAAATGGTTTATTTTGGTTGGAATGTAACTTAGTGAACAAGCCTAATTTCGAAGAAACAAAAGGAATGATTTGGATTTATATTGGAAAATGCAGAAGGAAAATGTTTATCGTAGTGTGCTTTAGTCATGTATTGGAATCAATTATTGGAATCAATACTGATGAACTCTGAAGTTGCCTTGCGCCTGTGTATTAGCTACCCTTTCAATAAATAGTATATGCACTTATCATCTTGATTGATTTTGTGACTTGGATCTTATGTTTGTGTTCGTAAAGAGTGATTACTAAAATTTTAATTCAAGTTTTCGTTTTGTCACAGGTATAAACCTGATGTAATTAAAGGTGACATGGACTCCATCAGAGAAGATGTTCTGGATTTTTATGCAAAATTGGTTAGTGGTTTCATTAGTCAGAATATCCTAAGAATAATGCTTTTAGGAAAGGCTGTTTTCGTGCTGGATTTATGTATGGCAACCAATTTTAGTATTTATCCAAGCTTATTCCATCTCGCTTATATATGGATTTGTTTAGGTGTTGAAAAGTTTAAACATTTCCAGAATGAGTTAGTGTTACTATTTAACTCCTGATTATGATGAATTAGATAAAATGATGTAGTAATTTGAATTCTACTGTTTTGTTTTGAATCAAAGGTCTATGGAAGATAGCCTCTCTAACTGGGAGCATAGAGATAATTGCTGTTTTCATACATCAGGCCTACAAGCACCTGTATCTGTATGTGGGAGCTTTGTGGTCTATGTAATTGATGTTATCGGTATATGTGCTTGTCTACTGTGCATGGGGTATAGTGAAGGACAGAAGAACTAAAAGTTTCCTGATATAAATGGGTTTTGAACTCTTAATCCCGTGTATTGATACTTTGATGGCGGTTAAGTCTTCGGTTCTTGTCACTTGAAATATGTACTCAAGCTAGTTGCTCCAAGCCTGTCTCAAAATCGTATTCCAGTTACTCTGAATTTCGTTGTCTCCTTTTAGTTCAGATAACGCTTAGTGGTTCATGTTGCTTGAATATATTCCGAGGATGCTCAATTTCTAGGTCATGTCGGAAAAACTTTGCTTGAGTGAAGGAAGTTTCACATATGACATGTCTAGGGGGCTTATGTGAGCTGCTTCTCTTATATTTGGGTAATGGAATTATAACTAGCTCTGGTTTTGTCAACCAAACAATTTTATGGCTTTCTGTATCGAGGGTATATATAACTTGGTTTTTTCTTGTAACTCAAATCATGCATGATGAATTACAACATAATAATGGTTTTTTAATGTTAATCATCTAGGTTTATCCTATGAAATTCTTTTTAAAATGTTTCCTTTCTCCATCTCTGTGCCCTTGCTAGCGCCATGCTTCCCTTTCTCTGTCTCTGATTCAAAATTTCCATAAGTAACTGATATTAACTTGATGGGAACATATGATAAACCAGTCTCTGATATTGATAACCAAGACTGAATCTCTTCACTTGCATGATCATAATCTGCTTATAAGAGATTGGCCATTGATTACCTGAGGCTGCTTAATGGCCATTGCAAATGTGAAAGCCCTTACTGTCTTGCATAATCCCGTCTCAGACGTTATTTAGTTTATGCCTCTACTTACTGTATTTCCCAACATACGTGATTTCTTGATCTTCCGCTTCTAAGCATGTTGTTACTTTAGGGCATTTGTCTGAGAACATGAGCTGTTTTTCTCCCCTTCTATGTCCTTGATTTCTACTTATATGTTAACTTGCTCTAATTGTGATTTTCCACAGTTTTTAAAAAGCGCATGATTCTGTTGGCTTATTGTTGACATTGCTACTGCTGGATTTCTGATGTCAGGGGTGTGATGCAATAAATGAATCTCATGACCAAGACACCACAGACCTACACAAGTGTATATCACATATCAGTAATTTGACACCAGAATCTGAGAAATCGAATGTAAGTTTCCTTCATATGCTCTTCAGCAGTTTACATCCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCCCCACCCCCTCTCTTGTTGTGTACAAAGCTATTGTGTTTATGCTCTGCCTGTAGTATATCCTTATCTGTTTTGTTGCAATCTGATCATATACATTTGTTACAAGCCTGGTAAATGAGCCGAAAACTGTTTTTCCCCCTTTTATTTCCTTCATAAAATTTGTGTTGGAGTTAATTGGTTGGTTGTTATGTACGTTGCAGCTGTGCATTCTTGTTACTGGAGCACTCGGTGGACGCTTTGATCATGAAGCGGGGAACATAAATGTTCTTTATCGTTTCTCTAACTTGCGAATAATACTTATTTCGGACGATTGCTTGATCCAACTTCTCCCAAAAACTCACCGCCATGAGATCCAAATTCAATCTTCTGTTGTTGGTCCACATTGTGGACTATTACCAATGGGACAGCCTGCTTTTCATACCACCACCTCTGGGCTGAAATGGAACCTCGGTAAGTAGATTTTTTGTGCTGTTCTATTTGTGCTAAACTGCATAGTTCATTTTCGCGCTTAGGTTTTTCTGTTATGTCATGATACGTTTTTGCTCGAATAACTGCTAAATTGTTGGTTAATCAGTGTATTTTCTCATTTTTTTGATTCCTTTTTCTTACCTCTTCTCTTGCTTGGGCTCTTGTTAACCCATATATGGAAAGAATATCAGGACATTCAGTATGGTAAAGAAAGCCATTAATTGTGCAATTAGCAGAACTGCTATCCTTATTAGCCAATTAGGTATACAAATGACTCAATTAGGGTTAGCCTAATCAAGATCCTCAAACTTAATAGCATGTCAATTAACTTGCTACATTCATTTCTTCCCTTAGTTTTGTGAAGTTTTTTTTTTCTAACTCTTAGATCTGCCTGAATATATATGCACCACTGCATGTCTGTATGTATTTGAACTATGTAACCTAATTTTAGTTTCTATGATACTAGCCCTATCAAAACTTACTTCAGATGTGAATTTTAACTTGCGGATCCTTATTTGAACCTACTTTCCCTTACTAGAACTTAATTTAATTGATTAGAACTTGTTAAACTTCAATTTTTTAAGATAAAAGAACATAATTTAAATGCTCCCTATGGTGTCAGATGTGATAACTGTCTCAAAATTTAGGTTTTTTTGCCAAAAAGGACCTTTTATAAACGTTTTTTTGCGAGAAAGGACCTTTTATGACGAAATTGGTGAAATGGGACCTAAAATATAATTTTTTTTGTTGATTGGGACCTTTTACCGGTTTCTTGGAGTTGATCCAAGATTCCGGCAACGACTTTGACACGTGGCAACCGGAGAAGGCCACGTGTCCATTTAATGATTAAAAAAAAAAAGTTTGCCCCCCCCCAAAAAAAAAAAAAAGTCCCCCGAAAAAAAAAAATTATCGATTTTTTTTTTGCCCCAAATAAATTTTTTTTTTTTTTTGCCCCAAATCAATTTTTTTTTTGCCCCAAATCGATTTTTTTTTGCTCCAATTCTCCCTCCAAAATCCCAATTGCCTTAAAGTTCGTCACTGAAATTGTCTGCCACGAATGAGGTGTGCTATTTTTTCCCCTCAATTTCCTCCTCAATTGTTCTAATTTTTGCGTCGAAAAAATTAGGGTTCTTCGCAAAATTGGGGCGAAAAAAATCGATTTGGGGCAAAAAAAAAAATTCGATAATTTTTTTTTTTTTGGGCAACTTTTTTTTTTAATCATTAAATGGACACGTGGCACTCTCCGGTTGCCACGTGTCAAAGTCGTTGCCGGAATCTTGGGTCAACTCCAAAAAACCGGTAAAAGGTCCCAATCAACAAAAAAAATTATGTTTTAGGTCCCATTTCACCAATTTCGTCATAAAAGGTCCTTTCTCGCAAAAAACCGTTTATAAAAGGTCCTTTTTGGCAAAAAGTCCCAAAATTTATCACCTTGGTTGTATTTTTGTTCCTTCTCCTTGCTCGGAGTGACAACGTGTGTCAGTTAAAGTATTGCGATTATCATAAACGGAGGAACAATAAAGAACGATGTCATTTCCCTCTATGCTTTGCTATTTTCCCTCTTTCAGTCCATTTTTATTTGCCATATATTGGGAAAGAGATTTACAAAGTAAGTAAACCAACATCATTTATTACATTCCCCTTTCTTCATTTATTGCCATATGAGGTATGAAATTGTGAATCACATGCTGCCAAAGGGGTACTAATGAATGACATGCAGTCCCATACTTATGCTTGATCCTGTATTGAGTGGTAGATATGTTGTATGCGTGCATCAGACTGCCTTCTATTATTTGTCGGCAGCCGACATTGCCATTCTTGAAAATGATATCCATGTGCTAAGGTTAGGTAAAACCACCATGTAGGTCTGTTTTATTCATCTAATCTGTTGTGACTTACTCCCTCCTTCTGTAATTGATATATGTATTATTTTAAAACTACTGTTAGAAGTTGTTGCCTTCCCTAAACAAAGAACATTTTATAATCGATTTTTTCAATTTGCCACCAGTATAATGTAAGCAATGGGGAGATTATAGAAATGACAACTGTTATGAAGGTTGGATTTATAAGAATGGTAACATCATGTCATAACCTTAATTACAAGGGGTTTAGGAAAATGTGACTATTAATCCCGGACAGATACGAGGGGGTTAGCTAGTTTCTGTTTGTTATGCTTATTGCTTATTTCTGTAAGTTTATCCCCCTCCGCCGGTGGCTCAGTGGTGTGATCTATGTGTTTTAAGTTTTAACATAATTTCCTCCCTCTATTCTTTCAGATGACACGGAGATGATGTTTGGCGGTTTGTTAAGTACATCTAATATAGTGACCGGAGATATAGTATCTGTGCAGTCTGATGCAGATCTTATTTGGACAATATCCATCAAACACTGATTTCACGTTTATGCAGTTTTAAGAGAACATCACATATGCTCTCATCACAAAAAATTTCTAACTTACTCTCAAGTCCATCAACTCGATCCTGCAATACAAAATAGTATCTGTTCACACAGATTTTGCGAAAATAAAGGTGTCAAGAAAGTTGAATAATCATCATTACTAAAGTGGAGATATTAGTATGGTTTAGAGCATTTACAGTTATGTTATCTTCAAATTCATTATATACTACATTTTTTTTTTTGAGGGGCATTATATACTACATTGTTACTACATAGTTGATTATTTGTATTGTCTATATTATCTTACAATCAATATTCTTTTCCATTTTTGTGAAAATAATGAAATATTTTTCATCTGTTCAATCTTTTCTTTTCTATAAGTCAAGTTAGCTGCAAGGCATTTAATTCACCATTTCCTGCATTCAAGTATGGGTTTGGTGGGGGTA

mRNA sequence

ATGAGTCGGTATGACCAAATTTATCAGAGACATAAAACAGAAACAGGGCTCACAAAAATCGACGAGTTAAGAAAAACGAAATACCAAACTCACCAATTCCGCCATAATTGGAAGAATTCCATGGTTTATTTAAACCAAACACCTCTCCTTCAAACTCCATTGACAAACATCAAGCTAATCTCCCAACTCAACAACAAATCCCAACAAATAAATTCCACTTTGAACCCTGATTATTACAGTAATAATATGGAAGCAATGCAACACTCTTCGTCTTTTCTTCTTCCTTCAATTCCTATTGAACAAGGTGCTAATTCGCTAAATTACGCCTTAGTTATTCTTAACCAACGCCTACCTCGTTTTGCCCCTCTTCTATGGGAGCACGCAAAATTGAGAGTTTGCGCAGATGGAGGAGGGAATCGTTTGTTCGATGAAATTCCGCAGTTTTTTCCTGAACAAAATCCCATTGATGTTCGCAACAGGTATAAACCTGATGTAATTAAAGGTGACATGGACTCCATCAGAGAAGATGTTCTGGATTTTTATGCAAAATTGGGGTGTGATGCAATAAATGAATCTCATGACCAAGACACCACAGACCTACACAAGTGTATATCACATATCAGTAATTTGACACCAGAATCTGAGAAATCGAATCTGTGCATTCTTGTTACTGGAGCACTCGGTGGACGCTTTGATCATGAAGCGGGGAACATAAATGTTCTTTATCGTTTCTCTAACTTGCGAATAATACTTATTTCGGACGATTGCTTGATCCAACTTCTCCCAAAAACTCACCGCCATGAGATCCAAATTCAATCTTCTGTTGTTGGTCCACATTGTGGACTATTACCAATGGGACAGCCTGCTTTTCATACCACCACCTCTGGGCTGAAATGGAACCTCGATGACACGGAGATGATGTTTGGCGGTTTGTTAAGTACATCTAATATAGTGACCGGAGATATAGTATCTGTGCAGTCTGATGCAGATCTTATTTGGACAATATCCATCAAACACTGATTTCACGTTTATGCAGTTTTAAGAGAACATCACATATGCTCTCATCACAAAAAATTTCTAACTTACTCTCAAGTCCATCAACTCGATCCTGCAATACAAAATAGTATCTGTTCACACAGATTTTGCGAAAATAAAGGTGTCAAGAAAGTTGAATAATCATCATTACTAAAGTGGAGATATTAGTATGGTTTAGAGCATTTACAGTTATGTTATCTTCAAATTCATTATATACTACATTTTTTTTTTTGAGGGGCATTATATACTACATTGTTACTACATAGTTGATTATTTGTATTGTCTATATTATCTTACAATCAATATTCTTTTCCATTTTTGTGAAAATAATGAAATATTTTTCATCTGTTCAATCTTTTCTTTTCTATAAGTCAAGTTAGCTGCAAGGCATTTAATTCACCATTTCCTGCATTCAAGTATGGGTTTGGTGGGGGTA

Coding sequence (CDS)

ATGAGTCGGTATGACCAAATTTATCAGAGACATAAAACAGAAACAGGGCTCACAAAAATCGACGAGTTAAGAAAAACGAAATACCAAACTCACCAATTCCGCCATAATTGGAAGAATTCCATGGTTTATTTAAACCAAACACCTCTCCTTCAAACTCCATTGACAAACATCAAGCTAATCTCCCAACTCAACAACAAATCCCAACAAATAAATTCCACTTTGAACCCTGATTATTACAGTAATAATATGGAAGCAATGCAACACTCTTCGTCTTTTCTTCTTCCTTCAATTCCTATTGAACAAGGTGCTAATTCGCTAAATTACGCCTTAGTTATTCTTAACCAACGCCTACCTCGTTTTGCCCCTCTTCTATGGGAGCACGCAAAATTGAGAGTTTGCGCAGATGGAGGAGGGAATCGTTTGTTCGATGAAATTCCGCAGTTTTTTCCTGAACAAAATCCCATTGATGTTCGCAACAGGTATAAACCTGATGTAATTAAAGGTGACATGGACTCCATCAGAGAAGATGTTCTGGATTTTTATGCAAAATTGGGGTGTGATGCAATAAATGAATCTCATGACCAAGACACCACAGACCTACACAAGTGTATATCACATATCAGTAATTTGACACCAGAATCTGAGAAATCGAATCTGTGCATTCTTGTTACTGGAGCACTCGGTGGACGCTTTGATCATGAAGCGGGGAACATAAATGTTCTTTATCGTTTCTCTAACTTGCGAATAATACTTATTTCGGACGATTGCTTGATCCAACTTCTCCCAAAAACTCACCGCCATGAGATCCAAATTCAATCTTCTGTTGTTGGTCCACATTGTGGACTATTACCAATGGGACAGCCTGCTTTTCATACCACCACCTCTGGGCTGAAATGGAACCTCGATGACACGGAGATGATGTTTGGCGGTTTGTTAAGTACATCTAATATAGTGACCGGAGATATAGTATCTGTGCAGTCTGATGCAGATCTTATTTGGACAATATCCATCAAACACTGA

Protein sequence

MSRYDQIYQRHKTETGLTKIDELRKTKYQTHQFRHNWKNSMVYLNQTPLLQTPLTNIKLISQLNNKSQQINSTLNPDYYSNNMEAMQHSSSFLLPSIPIEQGANSLNYALVILNQRLPRFAPLLWEHAKLRVCADGGGNRLFDEIPQFFPEQNPIDVRNRYKPDVIKGDMDSIREDVLDFYAKLGCDAINESHDQDTTDLHKCISHISNLTPESEKSNLCILVTGALGGRFDHEAGNINVLYRFSNLRIILISDDCLIQLLPKTHRHEIQIQSSVVGPHCGLLPMGQPAFHTTTSGLKWNLDDTEMMFGGLLSTSNIVTGDIVSVQSDADLIWTISIKH

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo07973.1	Spo07973.1	mRNA

Homology

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BLAST of Spo07973.1 vs. NCBI nr
Match: gi|902226912|gb|KNA20522.1| (hypothetical protein SOVF_051370 isoform B [Spinacia oleracea])

HSP 1 Score: 524.6 bits (1350), Expect = 1.300e-145
Identity = 254/254 (100.00%), Postives = 254/254 (100.00%), Query Frame = 1


		  
Query: 86  MQHSSSFLLPSIPIEQGANSLNYALVILNQRLPRFAPLLWEHAKLRVCADGGGNRLFDEI 145
           MQHSSSFLLPSIPIEQGANSLNYALVILNQRLPRFAPLLWEHAKLRVCADGGGNRLFDEI
Sbjct: 1   MQHSSSFLLPSIPIEQGANSLNYALVILNQRLPRFAPLLWEHAKLRVCADGGGNRLFDEI 60

Query: 146 PQFFPEQNPIDVRNRYKPDVIKGDMDSIREDVLDFYAKLGCDAINESHDQDTTDLHKCIS 205
           PQFFPEQNPIDVRNRYKPDVIKGDMDSIREDVLDFYAKLGCDAINESHDQDTTDLHKCIS
Sbjct: 61  PQFFPEQNPIDVRNRYKPDVIKGDMDSIREDVLDFYAKLGCDAINESHDQDTTDLHKCIS 120

Query: 206 HISNLTPESEKSNLCILVTGALGGRFDHEAGNINVLYRFSNLRIILISDDCLIQLLPKTH 265
           HISNLTPESEKSNLCILVTGALGGRFDHEAGNINVLYRFSNLRIILISDDCLIQLLPKTH
Sbjct: 121 HISNLTPESEKSNLCILVTGALGGRFDHEAGNINVLYRFSNLRIILISDDCLIQLLPKTH 180

Query: 266 RHEIQIQSSVVGPHCGLLPMGQPAFHTTTSGLKWNLDDTEMMFGGLLSTSNIVTGDIVSV 325
           RHEIQIQSSVVGPHCGLLPMGQPAFHTTTSGLKWNLDDTEMMFGGLLSTSNIVTGDIVSV
Sbjct: 181 RHEIQIQSSVVGPHCGLLPMGQPAFHTTTSGLKWNLDDTEMMFGGLLSTSNIVTGDIVSV 240

Query: 326 QSDADLIWTISIKH 340
           QSDADLIWTISIKH
Sbjct: 241 QSDADLIWTISIKH 254

[Close]

BLAST of Spo07973.1 vs. NCBI nr
Match: gi|731318589|ref|XP_010669808.1| (PREDICTED: thiamine pyrophosphokinase 2-like isoform X1 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 459.1 bits (1180), Expect = 6.500e-126
Identity = 217/256 (84.77%), Postives = 235/256 (91.80%), Query Frame = 1


		  
Query: 83  MEAMQHSSSFLLPSIPIEQGANSLNYALVILNQRLPRFAPLLWEHAKLRVCADGGGNRLF 142
           ME M HS++FLLP IP EQG  SL YALVILNQRLPRF PLLWEHAKLR+CADGG NRLF
Sbjct: 1   MEVMHHSTAFLLPLIPSEQGF-SLKYALVILNQRLPRFTPLLWEHAKLRICADGGANRLF 60

Query: 143 DEIPQFFPEQNPIDVRNRYKPDVIKGDMDSIREDVLDFYAKLGCDAINESHDQDTTDLHK 202
           DE+PQFFP+QNP+DVRNRYKPD+IKGDMDSIR +VLDFYAKLGCDAINESHDQDTTDLHK
Sbjct: 61  DELPQFFPQQNPLDVRNRYKPDLIKGDMDSIRSEVLDFYAKLGCDAINESHDQDTTDLHK 120

Query: 203 CISHISNLTPESEKSNLCILVTGALGGRFDHEAGNINVLYRFSNLRIILISDDCLIQLLP 262
           CISH+SNLTPESEK+NLCILVTGALGGRFDHEAGNINVLYRF  LRIIL+SDDCLIQLLP
Sbjct: 121 CISHLSNLTPESEKANLCILVTGALGGRFDHEAGNINVLYRFPGLRIILLSDDCLIQLLP 180

Query: 263 KTHRHEIQIQSSVVGPHCGLLPMGQPAFHTTTSGLKWNLDDTEMMFGGLLSTSNIVTGDI 322
           + HRHEI IQSSVVGPHCG+LP+G PAFHTTTSGL+WNL D EM+FGGL+STSN+VTGDI
Sbjct: 181 RAHRHEILIQSSVVGPHCGVLPIGSPAFHTTTSGLEWNLTDAEMVFGGLISTSNLVTGDI 240

Query: 323 VSVQSDADLIWTISIK 339
           V V SD DLIWTI IK
Sbjct: 241 VCVHSDTDLIWTICIK 255

[Close]

BLAST of Spo07973.1 vs. NCBI nr
Match: gi|902226911|gb|KNA20521.1| (hypothetical protein SOVF_051370 isoform A [Spinacia oleracea])

HSP 1 Score: 441.0 bits (1133), Expect = 1.800e-120
Identity = 212/212 (100.00%), Postives = 212/212 (100.00%), Query Frame = 1


		  
Query: 128 AKLRVCADGGGNRLFDEIPQFFPEQNPIDVRNRYKPDVIKGDMDSIREDVLDFYAKLGCD 187
           AKLRVCADGGGNRLFDEIPQFFPEQNPIDVRNRYKPDVIKGDMDSIREDVLDFYAKLGCD
Sbjct: 6   AKLRVCADGGGNRLFDEIPQFFPEQNPIDVRNRYKPDVIKGDMDSIREDVLDFYAKLGCD 65

Query: 188 AINESHDQDTTDLHKCISHISNLTPESEKSNLCILVTGALGGRFDHEAGNINVLYRFSNL 247
           AINESHDQDTTDLHKCISHISNLTPESEKSNLCILVTGALGGRFDHEAGNINVLYRFSNL
Sbjct: 66  AINESHDQDTTDLHKCISHISNLTPESEKSNLCILVTGALGGRFDHEAGNINVLYRFSNL 125

Query: 248 RIILISDDCLIQLLPKTHRHEIQIQSSVVGPHCGLLPMGQPAFHTTTSGLKWNLDDTEMM 307
           RIILISDDCLIQLLPKTHRHEIQIQSSVVGPHCGLLPMGQPAFHTTTSGLKWNLDDTEMM
Sbjct: 126 RIILISDDCLIQLLPKTHRHEIQIQSSVVGPHCGLLPMGQPAFHTTTSGLKWNLDDTEMM 185

Query: 308 FGGLLSTSNIVTGDIVSVQSDADLIWTISIKH 340
           FGGLLSTSNIVTGDIVSVQSDADLIWTISIKH
Sbjct: 186 FGGLLSTSNIVTGDIVSVQSDADLIWTISIKH 217

[Close]

BLAST of Spo07973.1 vs. NCBI nr
Match: gi|641868590|gb|KDO87274.1| (hypothetical protein CISIN_1g047888mg [Citrus sinensis])

HSP 1 Score: 394.4 bits (1012), Expect = 2.000e-106
Identity = 184/257 (71.60%), Postives = 223/257 (86.77%), Query Frame = 1


		  
Query: 83  MEAMQHSSSFLLPSIPIEQGANSLNYALVILNQRLPRFAPLLWEHAKLRVCADGGGNRLF 142
           ME M HSSSFLLPS P +    SL YALV+LNQRLPRFAPLLW+HAKLR+CADGG NR++
Sbjct: 18  MELMTHSSSFLLPSTPTDSRP-SLTYALVVLNQRLPRFAPLLWQHAKLRLCADGGANRVY 77

Query: 143 DEIPQFFPEQNPIDVRNRYKPDVIKGDMDSIREDVLDFYAKLGCDAINESHDQDTTDLHK 202
           DE+PQ FP ++P D+R RYKPD+IKGDMDSIR++V+DFYA LG   ++ESHDQDTTDLHK
Sbjct: 78  DELPQLFPHEDPSDIRRRYKPDLIKGDMDSIRKEVMDFYASLGTKVVDESHDQDTTDLHK 137

Query: 203 CISHISNLTPESEKSNLCILVTGALGGRFDHEAGNINVLYRFSNLRIILISDDCLIQLLP 262
           C+++I + TP  EKSNL ILV GALGGRFDHEAGNINVLYRFS++RIIL+SDDC IQLLP
Sbjct: 138 CVAYIRDCTPNLEKSNLRILVAGALGGRFDHEAGNINVLYRFSDIRIILLSDDCHIQLLP 197

Query: 263 KTHRHEIQIQSSVVGPHCGLLPMGQPAFHTTTSGLKWNLDDTEMMFGGLLSTSNIVTGDI 322
           KTHRH+I IQSSV GPHCGL+P+G P+  T+T+GL+W+LD+TE  FGGL+STSNIV G+ 
Sbjct: 198 KTHRHDIYIQSSVEGPHCGLIPIGMPSGSTSTTGLQWDLDNTETRFGGLVSTSNIVKGEK 257

Query: 323 VSVQSDADLIWTISIKH 340
           V+V+SD+DL+WTISIK+
Sbjct: 258 VTVRSDSDLLWTISIKN 273

[Close]

BLAST of Spo07973.1 vs. NCBI nr
Match: gi|567903618|ref|XP_006444297.1| (hypothetical protein CICLE_v10021279mg [Citrus clementina])

HSP 1 Score: 394.4 bits (1012), Expect = 2.000e-106
Identity = 184/257 (71.60%), Postives = 223/257 (86.77%), Query Frame = 1


		  
Query: 83  MEAMQHSSSFLLPSIPIEQGANSLNYALVILNQRLPRFAPLLWEHAKLRVCADGGGNRLF 142
           ME M HSSSFLLPS P +    SL YALV+LNQRLPRFAPLLW+HAKLR+CADGG NR++
Sbjct: 42  MELMTHSSSFLLPSTPTDSRP-SLTYALVVLNQRLPRFAPLLWQHAKLRLCADGGANRVY 101

Query: 143 DEIPQFFPEQNPIDVRNRYKPDVIKGDMDSIREDVLDFYAKLGCDAINESHDQDTTDLHK 202
           DE+PQ FP ++P D+R RYKPD+IKGDMDSIR++V+DFYA LG   ++ESHDQDTTDLHK
Sbjct: 102 DELPQLFPHEDPSDIRRRYKPDLIKGDMDSIRKEVMDFYASLGTKVVDESHDQDTTDLHK 161

Query: 203 CISHISNLTPESEKSNLCILVTGALGGRFDHEAGNINVLYRFSNLRIILISDDCLIQLLP 262
           C+++I + TP  EKSNL ILV GALGGRFDHEAGNINVLYRFS++RIIL+SDDC IQLLP
Sbjct: 162 CVAYIRDCTPNLEKSNLRILVAGALGGRFDHEAGNINVLYRFSDIRIILLSDDCHIQLLP 221

Query: 263 KTHRHEIQIQSSVVGPHCGLLPMGQPAFHTTTSGLKWNLDDTEMMFGGLLSTSNIVTGDI 322
           KTHRH+I IQSSV GPHCGL+P+G P+  T+T+GL+W+LD+TE  FGGL+STSNIV G+ 
Sbjct: 222 KTHRHDIYIQSSVEGPHCGLIPIGMPSGSTSTTGLEWDLDNTETRFGGLVSTSNIVKGEK 281

Query: 323 VSVQSDADLIWTISIKH 340
           V+V+SD+DL+WTISIK+
Sbjct: 282 VTVRSDSDLLWTISIKN 297

[Close]

BLAST of Spo07973.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RNT1_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_051370 PE=4 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 8.800e-146
Identity = 254/254 (100.00%), Postives = 254/254 (100.00%), Query Frame = 1


		  
Query: 86  MQHSSSFLLPSIPIEQGANSLNYALVILNQRLPRFAPLLWEHAKLRVCADGGGNRLFDEI 145
           MQHSSSFLLPSIPIEQGANSLNYALVILNQRLPRFAPLLWEHAKLRVCADGGGNRLFDEI
Sbjct: 1   MQHSSSFLLPSIPIEQGANSLNYALVILNQRLPRFAPLLWEHAKLRVCADGGGNRLFDEI 60

Query: 146 PQFFPEQNPIDVRNRYKPDVIKGDMDSIREDVLDFYAKLGCDAINESHDQDTTDLHKCIS 205
           PQFFPEQNPIDVRNRYKPDVIKGDMDSIREDVLDFYAKLGCDAINESHDQDTTDLHKCIS
Sbjct: 61  PQFFPEQNPIDVRNRYKPDVIKGDMDSIREDVLDFYAKLGCDAINESHDQDTTDLHKCIS 120

Query: 206 HISNLTPESEKSNLCILVTGALGGRFDHEAGNINVLYRFSNLRIILISDDCLIQLLPKTH 265
           HISNLTPESEKSNLCILVTGALGGRFDHEAGNINVLYRFSNLRIILISDDCLIQLLPKTH
Sbjct: 121 HISNLTPESEKSNLCILVTGALGGRFDHEAGNINVLYRFSNLRIILISDDCLIQLLPKTH 180

Query: 266 RHEIQIQSSVVGPHCGLLPMGQPAFHTTTSGLKWNLDDTEMMFGGLLSTSNIVTGDIVSV 325
           RHEIQIQSSVVGPHCGLLPMGQPAFHTTTSGLKWNLDDTEMMFGGLLSTSNIVTGDIVSV
Sbjct: 181 RHEIQIQSSVVGPHCGLLPMGQPAFHTTTSGLKWNLDDTEMMFGGLLSTSNIVTGDIVSV 240

Query: 326 QSDADLIWTISIKH 340
           QSDADLIWTISIKH
Sbjct: 241 QSDADLIWTISIKH 254

[Close]

BLAST of Spo07973.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CUW1_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g037030 PE=4 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 4.500e-126
Identity = 217/256 (84.77%), Postives = 235/256 (91.80%), Query Frame = 1


		  
Query: 83  MEAMQHSSSFLLPSIPIEQGANSLNYALVILNQRLPRFAPLLWEHAKLRVCADGGGNRLF 142
           ME M HS++FLLP IP EQG  SL YALVILNQRLPRF PLLWEHAKLR+CADGG NRLF
Sbjct: 1   MEVMHHSTAFLLPLIPSEQGF-SLKYALVILNQRLPRFTPLLWEHAKLRICADGGANRLF 60

Query: 143 DEIPQFFPEQNPIDVRNRYKPDVIKGDMDSIREDVLDFYAKLGCDAINESHDQDTTDLHK 202
           DE+PQFFP+QNP+DVRNRYKPD+IKGDMDSIR +VLDFYAKLGCDAINESHDQDTTDLHK
Sbjct: 61  DELPQFFPQQNPLDVRNRYKPDLIKGDMDSIRSEVLDFYAKLGCDAINESHDQDTTDLHK 120

Query: 203 CISHISNLTPESEKSNLCILVTGALGGRFDHEAGNINVLYRFSNLRIILISDDCLIQLLP 262
           CISH+SNLTPESEK+NLCILVTGALGGRFDHEAGNINVLYRF  LRIIL+SDDCLIQLLP
Sbjct: 121 CISHLSNLTPESEKANLCILVTGALGGRFDHEAGNINVLYRFPGLRIILLSDDCLIQLLP 180

Query: 263 KTHRHEIQIQSSVVGPHCGLLPMGQPAFHTTTSGLKWNLDDTEMMFGGLLSTSNIVTGDI 322
           + HRHEI IQSSVVGPHCG+LP+G PAFHTTTSGL+WNL D EM+FGGL+STSN+VTGDI
Sbjct: 181 RAHRHEILIQSSVVGPHCGVLPIGSPAFHTTTSGLEWNLTDAEMVFGGLISTSNLVTGDI 240

Query: 323 VSVQSDADLIWTISIK 339
           V V SD DLIWTI IK
Sbjct: 241 VCVHSDTDLIWTICIK 255

[Close]

BLAST of Spo07973.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RLY0_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_051370 PE=4 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 1.300e-120
Identity = 212/212 (100.00%), Postives = 212/212 (100.00%), Query Frame = 1


		  
Query: 128 AKLRVCADGGGNRLFDEIPQFFPEQNPIDVRNRYKPDVIKGDMDSIREDVLDFYAKLGCD 187
           AKLRVCADGGGNRLFDEIPQFFPEQNPIDVRNRYKPDVIKGDMDSIREDVLDFYAKLGCD
Sbjct: 6   AKLRVCADGGGNRLFDEIPQFFPEQNPIDVRNRYKPDVIKGDMDSIREDVLDFYAKLGCD 65

Query: 188 AINESHDQDTTDLHKCISHISNLTPESEKSNLCILVTGALGGRFDHEAGNINVLYRFSNL 247
           AINESHDQDTTDLHKCISHISNLTPESEKSNLCILVTGALGGRFDHEAGNINVLYRFSNL
Sbjct: 66  AINESHDQDTTDLHKCISHISNLTPESEKSNLCILVTGALGGRFDHEAGNINVLYRFSNL 125

Query: 248 RIILISDDCLIQLLPKTHRHEIQIQSSVVGPHCGLLPMGQPAFHTTTSGLKWNLDDTEMM 307
           RIILISDDCLIQLLPKTHRHEIQIQSSVVGPHCGLLPMGQPAFHTTTSGLKWNLDDTEMM
Sbjct: 126 RIILISDDCLIQLLPKTHRHEIQIQSSVVGPHCGLLPMGQPAFHTTTSGLKWNLDDTEMM 185

Query: 308 FGGLLSTSNIVTGDIVSVQSDADLIWTISIKH 340
           FGGLLSTSNIVTGDIVSVQSDADLIWTISIKH
Sbjct: 186 FGGLLSTSNIVTGDIVSVQSDADLIWTISIKH 217

[Close]

BLAST of Spo07973.1 vs. UniProtKB/TrEMBL
Match: A0A067H609_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g047888mg PE=4 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 1.400e-106
Identity = 184/257 (71.60%), Postives = 223/257 (86.77%), Query Frame = 1


		  
Query: 83  MEAMQHSSSFLLPSIPIEQGANSLNYALVILNQRLPRFAPLLWEHAKLRVCADGGGNRLF 142
           ME M HSSSFLLPS P +    SL YALV+LNQRLPRFAPLLW+HAKLR+CADGG NR++
Sbjct: 18  MELMTHSSSFLLPSTPTDSRP-SLTYALVVLNQRLPRFAPLLWQHAKLRLCADGGANRVY 77

Query: 143 DEIPQFFPEQNPIDVRNRYKPDVIKGDMDSIREDVLDFYAKLGCDAINESHDQDTTDLHK 202
           DE+PQ FP ++P D+R RYKPD+IKGDMDSIR++V+DFYA LG   ++ESHDQDTTDLHK
Sbjct: 78  DELPQLFPHEDPSDIRRRYKPDLIKGDMDSIRKEVMDFYASLGTKVVDESHDQDTTDLHK 137

Query: 203 CISHISNLTPESEKSNLCILVTGALGGRFDHEAGNINVLYRFSNLRIILISDDCLIQLLP 262
           C+++I + TP  EKSNL ILV GALGGRFDHEAGNINVLYRFS++RIIL+SDDC IQLLP
Sbjct: 138 CVAYIRDCTPNLEKSNLRILVAGALGGRFDHEAGNINVLYRFSDIRIILLSDDCHIQLLP 197

Query: 263 KTHRHEIQIQSSVVGPHCGLLPMGQPAFHTTTSGLKWNLDDTEMMFGGLLSTSNIVTGDI 322
           KTHRH+I IQSSV GPHCGL+P+G P+  T+T+GL+W+LD+TE  FGGL+STSNIV G+ 
Sbjct: 198 KTHRHDIYIQSSVEGPHCGLIPIGMPSGSTSTTGLQWDLDNTETRFGGLVSTSNIVKGEK 257

Query: 323 VSVQSDADLIWTISIKH 340
           V+V+SD+DL+WTISIK+
Sbjct: 258 VTVRSDSDLLWTISIKN 273

[Close]

BLAST of Spo07973.1 vs. UniProtKB/TrEMBL
Match: V4TVG9_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10021279mg PE=4 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 1.400e-106
Identity = 184/257 (71.60%), Postives = 223/257 (86.77%), Query Frame = 1


		  
Query: 83  MEAMQHSSSFLLPSIPIEQGANSLNYALVILNQRLPRFAPLLWEHAKLRVCADGGGNRLF 142
           ME M HSSSFLLPS P +    SL YALV+LNQRLPRFAPLLW+HAKLR+CADGG NR++
Sbjct: 42  MELMTHSSSFLLPSTPTDSRP-SLTYALVVLNQRLPRFAPLLWQHAKLRLCADGGANRVY 101

Query: 143 DEIPQFFPEQNPIDVRNRYKPDVIKGDMDSIREDVLDFYAKLGCDAINESHDQDTTDLHK 202
           DE+PQ FP ++P D+R RYKPD+IKGDMDSIR++V+DFYA LG   ++ESHDQDTTDLHK
Sbjct: 102 DELPQLFPHEDPSDIRRRYKPDLIKGDMDSIRKEVMDFYASLGTKVVDESHDQDTTDLHK 161

Query: 203 CISHISNLTPESEKSNLCILVTGALGGRFDHEAGNINVLYRFSNLRIILISDDCLIQLLP 262
           C+++I + TP  EKSNL ILV GALGGRFDHEAGNINVLYRFS++RIIL+SDDC IQLLP
Sbjct: 162 CVAYIRDCTPNLEKSNLRILVAGALGGRFDHEAGNINVLYRFSDIRIILLSDDCHIQLLP 221

Query: 263 KTHRHEIQIQSSVVGPHCGLLPMGQPAFHTTTSGLKWNLDDTEMMFGGLLSTSNIVTGDI 322
           KTHRH+I IQSSV GPHCGL+P+G P+  T+T+GL+W+LD+TE  FGGL+STSNIV G+ 
Sbjct: 222 KTHRHDIYIQSSVEGPHCGLIPIGMPSGSTSTTGLEWDLDNTETRFGGLVSTSNIVKGEK 281

Query: 323 VSVQSDADLIWTISIKH 340
           V+V+SD+DL+WTISIK+
Sbjct: 282 VTVRSDSDLLWTISIKN 297

[Close]

BLAST of Spo07973.1 vs. ExPASy Swiss-Prot
Match: TPK2_ARATH (Thiamine pyrophosphokinase 2 OS=Arabidopsis thaliana GN=TPK2 PE=2 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 4.200e-101
Identity = 175/256 (68.36%), Postives = 207/256 (80.86%), Query Frame = 1


		  
Query: 83  MEAMQHSSSFLLPSIPIEQGANSLNYALVILNQRLPRFAPLLWEHAKLRVCADGGGNRLF 142
           M+ M HSSSFLLP            YALV+LNQ LPRF PLLWEHAKLR+CADGG NR++
Sbjct: 5   MDVMIHSSSFLLPC----DETCGTRYALVVLNQNLPRFTPLLWEHAKLRLCADGGANRIY 64

Query: 143 DEIPQFFPEQNPIDVRNRYKPDVIKGDMDSIREDVLDFYAKLGCDAINESHDQDTTDLHK 202
           DE+P FFP ++P  +RNRYKPDVIKGDMDSIR DVLDFY   G   I+ESHDQDTTDL K
Sbjct: 65  DELPLFFPHEDPFVIRNRYKPDVIKGDMDSIRRDVLDFYVYWGTKVIDESHDQDTTDLDK 124

Query: 203 CISHISNLTPESEKSNLCILVTGALGGRFDHEAGNINVLYRFSNLRIILISDDCLIQLLP 262
           CIS+I + T   E S L IL TGALGGRFDHEAGN+NVLYR+ + RI+L+SDDCLIQLLP
Sbjct: 125 CISYIRHSTLNQESSRLQILATGALGGRFDHEAGNLNVLYRYPDTRIVLLSDDCLIQLLP 184

Query: 263 KTHRHEIQIQSSVVGPHCGLLPMGQPAFHTTTSGLKWNLDDTEMMFGGLLSTSNIVTGDI 322
           KTHRHEI I SS+ GPHCGL+P+G P+ +TTTSGLKW+L +TEM FGGL+STSN+V  +I
Sbjct: 185 KTHRHEIHIHSSLQGPHCGLIPIGTPSANTTTSGLKWDLSNTEMRFGGLISTSNLVKEEI 244

Query: 323 VSVQSDADLIWTISIK 339
           ++V+SD+DL+WTISIK
Sbjct: 245 ITVESDSDLLWTISIK 256

[Close]

BLAST of Spo07973.1 vs. ExPASy Swiss-Prot
Match: TPK1_ARATH (Thiamine pyrophosphokinase 1 OS=Arabidopsis thaliana GN=TPK1 PE=2 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 1.600e-100
Identity = 173/256 (67.58%), Postives = 209/256 (81.64%), Query Frame = 1


		  
Query: 83  MEAMQHSSSFLLPSIPIEQGANSLNYALVILNQRLPRFAPLLWEHAKLRVCADGGGNRLF 142
           M+ M HSSSFLLP    ++ +    YALV+LNQ LPRF PLLWEHAKLR+CADGG NR++
Sbjct: 4   MDVMIHSSSFLLPC---DETSTGTRYALVVLNQSLPRFTPLLWEHAKLRLCADGGANRIY 63

Query: 143 DEIPQFFPEQNPIDVRNRYKPDVIKGDMDSIREDVLDFYAKLGCDAINESHDQDTTDLHK 202
           DE+P FFP ++ + +RNRYKPDVIKGDMDSIR DVLDFY  LG   I+ESHDQDTTDL K
Sbjct: 64  DELPLFFPNEDALAIRNRYKPDVIKGDMDSIRRDVLDFYINLGTKVIDESHDQDTTDLDK 123

Query: 203 CISHISNLTPESEKSNLCILVTGALGGRFDHEAGNINVLYRFSNLRIILISDDCLIQLLP 262
           CI +I + T   E S L IL TGALGGRFDHEAGN+NVLYR+ + RI+L+SDDCLIQLLP
Sbjct: 124 CILYIRHSTLNQETSGLQILATGALGGRFDHEAGNLNVLYRYPDTRIVLLSDDCLIQLLP 183

Query: 263 KTHRHEIQIQSSVVGPHCGLLPMGQPAFHTTTSGLKWNLDDTEMMFGGLLSTSNIVTGDI 322
           KTHRHEI IQSS+ GPHCGL+P+G P+  TTTSGL+W+L +TEM FGGL+STSN+V  + 
Sbjct: 184 KTHRHEIHIQSSLEGPHCGLIPIGTPSAKTTTSGLQWDLSNTEMRFGGLISTSNLVKEEK 243

Query: 323 VSVQSDADLIWTISIK 339
           ++V+SD+DL+WTISIK
Sbjct: 244 ITVESDSDLLWTISIK 256

[Close]

BLAST of Spo07973.1 vs. ExPASy Swiss-Prot
Match: TPK1_ORYSJ (Thiamine pyrophosphokinase 1 OS=Oryza sativa subsp. japonica GN=TPK1 PE=2 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 9.100e-96
Identity = 168/262 (64.12%), Postives = 207/262 (79.01%), Query Frame = 1


		  
Query: 83  MEAMQHSSSFL-LPSI----PIEQGANSLNYALVILNQRLPRFAPLLWEHAKLRVCADGG 142
           +  M HSSSFL LP+     P      S  YA+V+LNQRLPRFAPLLW+ A+LRVCADGG
Sbjct: 3   LPTMTHSSSFLRLPATSSPHPPPADDASAAYAVVVLNQRLPRFAPLLWDRARLRVCADGG 62

Query: 143 GNRLFDEIPQFFPEQNPIDVRNRYKPDVIKGDMDSIREDVLDFYAKLGCDAINESHDQDT 202
            NR+FD +P+  P ++P  VR RYKPDVIKGDMDSIR +V ++Y+ LG + ++ESHDQDT
Sbjct: 63  ANRVFDGMPELLPAEDPDQVRMRYKPDVIKGDMDSIRPEVKEYYSNLGAEIVDESHDQDT 122

Query: 203 TDLHKCISHISNLTPESEKSNLCILVTGALGGRFDHEAGNINVLYRFSNLRIILISDDCL 262
           TDLHKC+S I+   P SE+SNL ILV GALGGRFDHE GNINVLYRFSN+RI+L+SDDC 
Sbjct: 123 TDLHKCVSFITRNPPGSEESNLYILVLGALGGRFDHEMGNINVLYRFSNIRIVLLSDDCS 182

Query: 263 IQLLPKTHRHEIQIQSSVVGPHCGLLPMGQPAFHTTTSGLKWNLDDTEMMFGGLLSTSNI 322
           I LLPKTH HEI I+ S+ GPHCGL+PMG P+  TTT+GL+WNLD+T M +GGL+STSNI
Sbjct: 183 IFLLPKTHSHEIHIERSIEGPHCGLIPMGSPSASTTTTGLRWNLDNTSMSYGGLISTSNI 242

Query: 323 VTGDIVSVQSDADLIWTISIKH 340
           V  + V + SD+DLIWTIS+++
Sbjct: 243 VEEETVRITSDSDLIWTISLRN 264

[Close]

BLAST of Spo07973.1 vs. ExPASy Swiss-Prot
Match: TPK2_ORYSJ (Thiamine pyrophosphokinase 2 OS=Oryza sativa subsp. japonica GN=TPK2 PE=2 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 9.500e-93
Identity = 158/258 (61.24%), Postives = 202/258 (78.29%), Query Frame = 1


		  
Query: 82  NMEAMQHSSSFLLPSIPIEQGANSLNYALVILNQRLPRFAPLLWEHAKLRVCADGGGNRL 141
           +ME M HSS+FLLP +     + +  YALV+LNQ LPRF P LW HAKLR+CADGG NR+
Sbjct: 8   SMEVMVHSSTFLLPKLHQPANSPAKKYALVVLNQNLPRFVPRLWTHAKLRICADGGANRI 67

Query: 142 FDEIPQFFPEQNPIDVRNRYKPDVIKGDMDSIREDVLDFYAKLGCDAINESHDQDTTDLH 201
           FDE+ Q   + +    R RY P++I+GDMDSIR +V  FY+  G    ++SH+Q+TTDLH
Sbjct: 68  FDEMFQMTNDPDYESTRKRYIPEIIEGDMDSIRPEVKQFYSSQGSKISDKSHNQETTDLH 127

Query: 202 KCISHISNLTPESEKSNLCILVTGALGGRFDHEAGNINVLYRFSNLRIILISDDCLIQLL 261
           KCIS I   TP+ EK+NLC+LVTGALGGRFDHEA NIN+LY FS++RI+L+SDDCLI+LL
Sbjct: 128 KCISRIHRCTPDHEKTNLCVLVTGALGGRFDHEAANINILYLFSDMRIVLLSDDCLIRLL 187

Query: 262 PKTHRHEIQIQSSVVGPHCGLLPMGQPAFHTTTSGLKWNLDDTEMMFGGLLSTSNIVTGD 321
           PKTH+HEI I+SSV GPHCGL P+G P+  TTT+GLKWNL + +M FG ++STSNIV  +
Sbjct: 188 PKTHKHEIYIESSVEGPHCGLFPVGAPSGSTTTTGLKWNLSEAKMRFGSMISTSNIVHAE 247

Query: 322 IVSVQSDADLIWTISIKH 340
            V+VQSDADL+WTIS+++
Sbjct: 248 KVTVQSDADLLWTISLRN 265

[Close]

BLAST of Spo07973.1 vs. ExPASy Swiss-Prot
Match: TPK3_ORYSJ (Thiamine pyrophosphokinase 3 OS=Oryza sativa subsp. japonica GN=TPK3 PE=2 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 3.600e-92
Identity = 159/260 (61.15%), Postives = 203/260 (78.08%), Query Frame = 1


		  
Query: 85  AMQHSSSFLLPSIPIEQG---ANSLNYALVILNQRLPRFAPLLWEHAKLRVCADGGGNRL 144
           AM HSS+FLLPS         A+   YAL++LNQRLPRFAP LW+ A++RVCADGG NR+
Sbjct: 6   AMSHSSAFLLPSPSAAAAGADADGAAYALLVLNQRLPRFAPRLWDRAQVRVCADGGANRV 65

Query: 145 FDEIPQFFPEQNPIDVRNRYKPDVIKGDMDSIREDVLDFYAKLGCDAINESHDQDTTDLH 204
           FD +P+ FP Q+P +VR RYKPDVIKGD+DS+R +V ++Y+ +G   ++ESHDQDTTDLH
Sbjct: 66  FDGMPELFPGQDPDEVRRRYKPDVIKGDLDSVRPEVKEYYSNMGTQIVDESHDQDTTDLH 125

Query: 205 KCISHISNLTPESEKSNLCILVTGALGGRFDHEAGNINVLYRFSNLRIILISDDCLIQLL 264
           KC++ I+  +    KSNLCI   GALGGRFDHE GNINVL+ F N RIIL+SDDCLI LL
Sbjct: 126 KCVAFITENSAIPNKSNLCIFALGALGGRFDHEMGNINVLHLFPNNRIILLSDDCLIFLL 185

Query: 265 PKTHRHEIQIQSSVVGPHCGLLPMGQPAFHTTTSGLKWNLDDTEMMFGGLLSTSNIVTGD 324
           P+TH H I I+ S+ GPHCGL+P+G P+  TTT+GL+WNLD+T M FGGL+STSNIV  +
Sbjct: 186 PRTHTHNIHIERSIEGPHCGLIPIGAPSATTTTTGLQWNLDNTSMSFGGLISTSNIVREE 245

Query: 325 --IVSVQSDADLIWTISIKH 340
             +V++ SD+DLIWTIS++H
Sbjct: 246 STVVTITSDSDLIWTISLRH 265

[Close]

BLAST of Spo07973.1 vs. TAIR (Arabidopsis)
Match: AT2G44750.2 (thiamin pyrophosphokinase 2)

HSP 1 Score: 369.4 bits (947), Expect = 2.400e-102
Identity = 175/256 (68.36%), Postives = 207/256 (80.86%), Query Frame = 1


		  
Query: 83  MEAMQHSSSFLLPSIPIEQGANSLNYALVILNQRLPRFAPLLWEHAKLRVCADGGGNRLF 142
           M+ M HSSSFLLP            YALV+LNQ LPRF PLLWEHAKLR+CADGG NR++
Sbjct: 5   MDVMIHSSSFLLPC----DETCGTRYALVVLNQNLPRFTPLLWEHAKLRLCADGGANRIY 64

Query: 143 DEIPQFFPEQNPIDVRNRYKPDVIKGDMDSIREDVLDFYAKLGCDAINESHDQDTTDLHK 202
           DE+P FFP ++P  +RNRYKPDVIKGDMDSIR DVLDFY   G   I+ESHDQDTTDL K
Sbjct: 65  DELPLFFPHEDPFVIRNRYKPDVIKGDMDSIRRDVLDFYVYWGTKVIDESHDQDTTDLDK 124

Query: 203 CISHISNLTPESEKSNLCILVTGALGGRFDHEAGNINVLYRFSNLRIILISDDCLIQLLP 262
           CIS+I + T   E S L IL TGALGGRFDHEAGN+NVLYR+ + RI+L+SDDCLIQLLP
Sbjct: 125 CISYIRHSTLNQESSRLQILATGALGGRFDHEAGNLNVLYRYPDTRIVLLSDDCLIQLLP 184

Query: 263 KTHRHEIQIQSSVVGPHCGLLPMGQPAFHTTTSGLKWNLDDTEMMFGGLLSTSNIVTGDI 322
           KTHRHEI I SS+ GPHCGL+P+G P+ +TTTSGLKW+L +TEM FGGL+STSN+V  +I
Sbjct: 185 KTHRHEIHIHSSLQGPHCGLIPIGTPSANTTTSGLKWDLSNTEMRFGGLISTSNLVKEEI 244

Query: 323 VSVQSDADLIWTISIK 339
           ++V+SD+DL+WTISIK
Sbjct: 245 ITVESDSDLLWTISIK 256

[Close]

BLAST of Spo07973.1 vs. TAIR (Arabidopsis)
Match: AT1G02880.3 (thiamin pyrophosphokinase1)

HSP 1 Score: 367.5 bits (942), Expect = 9.100e-102
Identity = 173/256 (67.58%), Postives = 209/256 (81.64%), Query Frame = 1


		  
Query: 83  MEAMQHSSSFLLPSIPIEQGANSLNYALVILNQRLPRFAPLLWEHAKLRVCADGGGNRLF 142
           M+ M HSSSFLLP    ++ +    YALV+LNQ LPRF PLLWEHAKLR+CADGG NR++
Sbjct: 4   MDVMIHSSSFLLPC---DETSTGTRYALVVLNQSLPRFTPLLWEHAKLRLCADGGANRIY 63

Query: 143 DEIPQFFPEQNPIDVRNRYKPDVIKGDMDSIREDVLDFYAKLGCDAINESHDQDTTDLHK 202
           DE+P FFP ++ + +RNRYKPDVIKGDMDSIR DVLDFY  LG   I+ESHDQDTTDL K
Sbjct: 64  DELPLFFPNEDALAIRNRYKPDVIKGDMDSIRRDVLDFYINLGTKVIDESHDQDTTDLDK 123

Query: 203 CISHISNLTPESEKSNLCILVTGALGGRFDHEAGNINVLYRFSNLRIILISDDCLIQLLP 262
           CI +I + T   E S L IL TGALGGRFDHEAGN+NVLYR+ + RI+L+SDDCLIQLLP
Sbjct: 124 CILYIRHSTLNQETSGLQILATGALGGRFDHEAGNLNVLYRYPDTRIVLLSDDCLIQLLP 183

Query: 263 KTHRHEIQIQSSVVGPHCGLLPMGQPAFHTTTSGLKWNLDDTEMMFGGLLSTSNIVTGDI 322
           KTHRHEI IQSS+ GPHCGL+P+G P+  TTTSGL+W+L +TEM FGGL+STSN+V  + 
Sbjct: 184 KTHRHEIHIQSSLEGPHCGLIPIGTPSAKTTTSGLQWDLSNTEMRFGGLISTSNLVKEEK 243

Query: 323 VSVQSDADLIWTISIK 339
           ++V+SD+DL+WTISIK
Sbjct: 244 ITVESDSDLLWTISIK 256

The following BLAST results are available for this feature:

BLAST of Spo07973.1 vs. NCBI nr
BLAST of Spo07973.1 vs. UniProtKB/TrEMBL
BLAST of Spo07973.1 vs. ExPASy Swiss-Prot
BLAST of Spo07973.1 vs. TAIR (Arabidopsis)

BLAST of Spo07973.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902226912\|gb\|KNA20522.1\|	1.3e-145	100.	hypothetical protein SOVF_0513...	[more]
gi\|731318589\|ref\|XP_010669808.1\|	6.5e-126	84.7	PREDICTED: thiamine pyrophosph...	[more]
gi\|902226911\|gb\|KNA20521.1\|	1.8e-120	100.	hypothetical protein SOVF_0513...	[more]
gi\|641868590\|gb\|KDO87274.1\|	2.0e-106	71.6	hypothetical protein CISIN_1g0...	[more]
gi\|567903618\|ref\|XP_006444297.1\|	2.0e-106	71.6	hypothetical protein CICLE_v10...	[more]

BLAST of Spo07973.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RNT1_SPIOL	8.8e-146	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8CUW1_BETVU	4.5e-126	84.7	Uncharacterized protein OS=Bet...	[more]
A0A0K9RLY0_SPIOL	1.3e-120	100.	Uncharacterized protein OS=Spi...	[more]
A0A067H609_CITSI	1.4e-106	71.6	Uncharacterized protein OS=Cit...	[more]
V4TVG9_9ROSI	1.4e-106	71.6	Uncharacterized protein OS=Cit...	[more]

BLAST of Spo07973.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
TPK2_ARATH	4.2e-101	68.3	Thiamine pyrophosphokinase 2 O...	[more]
TPK1_ARATH	1.6e-100	67.5	Thiamine pyrophosphokinase 1 O...	[more]
TPK1_ORYSJ	9.1e-96	64.1	Thiamine pyrophosphokinase 1 O...	[more]
TPK2_ORYSJ	9.5e-93	61.2	Thiamine pyrophosphokinase 2 O...	[more]
TPK3_ORYSJ	3.6e-92	61.1	Thiamine pyrophosphokinase 3 O...	[more]

BLAST of Spo07973.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 2


Match Name	E-value	Identity	Description
AT2G44750.2	2.4e-102	68.3	thiamin pyrophosphokinase 2	[more]
AT1G02880.3	9.1e-102	67.5	thiamin pyrophosphokinase1	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006282	Thiamin pyrophosphokinase	TIGRFAMs	TIGR01378	TIGR01378	coord: 110..335 score: 1.4
IPR007371	Thiamin pyrophosphokinase, catalytic domain	GENE3D	3.40.50.10240		coord: 108..249 score: 9.9
IPR007371	Thiamin pyrophosphokinase, catalytic domain	PFAM	PF04263	TPK_catalytic	coord: 122..249 score: 7.5
IPR007371	Thiamin pyrophosphokinase, catalytic domain	unknown	SSF63999	Thiamin pyrophosphokinase, catalytic domain	coord: 100..257 score: 1.31
IPR007373	Thiamin pyrophosphokinase, vitamin B1-binding domain	GENE3D	2.60.120.320		coord: 250..338 score: 2.4
IPR007373	Thiamin pyrophosphokinase, vitamin B1-binding domain	PFAM	PF04265	TPK_B1_binding	coord: 266..332 score: 6.5
IPR007373	Thiamin pyrophosphokinase, vitamin B1-binding domain	SMART	SM00983	TPK_B1_binding_a_2_a	coord: 265..331 score: 1.3
IPR007373	Thiamin pyrophosphokinase, vitamin B1-binding domain	unknown	SSF63862	Thiamin pyrophosphokinase, substrate-binding domain	coord: 260..339 score: 4.19
None	No IPR available	PANTHER	PTHR13622	THIAMIN PYROPHOSPHOKINASE	coord: 83..337 score: 1.7E
None	No IPR available	PANTHER	PTHR13622:SF7	SUBFAMILY NOT NAMED	coord: 83..337 score: 1.7E

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
biological_process	GO:0044262	cellular carbohydrate metabolic process
biological_process	GO:0006449	regulation of translational termination
biological_process	GO:0006783	heme biosynthetic process
biological_process	GO:0051083	'de novo' cotranslational protein folding
biological_process	GO:0009229	thiamine diphosphate biosynthetic process
biological_process	GO:0044260	cellular macromolecule metabolic process
biological_process	GO:0009987	cellular process
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0006772	thiamine metabolic process
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0005840	ribosome
cellular_component	GO:0018444	translation release factor complex
molecular_function	GO:0004788	thiamine diphosphokinase activity
molecular_function	GO:0030975	thiamine binding
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0000166	nucleotide binding
molecular_function	GO:0016301	kinase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003747	translation release factor activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo07235	0.67	Barchart \| Table
Spo18184	0.67	Barchart \| Table
Spo18862	0.66	Barchart \| Table
Spo02826	0.65	Barchart \| Table
Spo15079	0.65	Barchart \| Table