Spo08120 (gene)

Overview
NameSpo08120
Typegene
OrganismSpinacia oleracea (Spinach)
DescriptionMembrane steroid-binding protein 2
Locationchr5 : 11187834 .. 11191156 (+)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGTATCTGAAAGAGGGTGACTCATACAGTCATACTCCGAATTTTTACTTATTCCATTTCAGGTTAATTGCAACATTAGGTGGAGATAAAGTTGAAGGAAAAATTTTTATCAAAAAAAAAAGCAAAAACAAAAAGTTGAAGGAAAATTTTAAAATTGTGAACTGATGAAGTGGTTTTTTAACTATGAATGTTTAGTAGATTTTTTAACAAATTGGGTTTTTTAAACTTTTAGGGCGTGTTCGGCGGCAGCTTTTGAGGTAGCGGGAAGCGTTTTGATCTAGGGCGTGTTCGACGATAGCTTTTGAGGTAGCGGGTAGCGTTTTGATCTAGGGCGTGTTTAGCGGTAGCTTTTGAGGTAGCGGGTAGCGTTTTGACCTAGGGCGTGTTCGGCGGTAACTTTTGAGGTAGCGGGTAACGGTTTGACCTATTCAAGACGCTACTTGTAAAATTTGTGAGTGTTTGGCAAGGTAGCGATTTAGGTAGCGGTTAATGGTAGAGGTAGCTTTTGTCAATCGTTAAAATTAGTACTCGGGTTTCACAAATTTAAGTAAAGTTTTAAACAATATTCTAACTTTTATTTTATTTTACAATCACAAATTCTTATTTACAATGGGTGTACAATAAATATTGTACACCAGAGTAAAAGTTAACTCAAAATGCTTAAAAGTTAAGCATACATATGTAAACGTTATCTATTTTTCAATGATAAATTTTTTCATTTTAGTAAAACTTATTTCTTCAAAATAACTAATAATGTATAAAATTAATCATTTAACCCTTTAAAATATTTATCTATCAATTTTTTTTACTAATATAAAAGTTAATCAAAACTAGGTTAAAGTTATAAAAAAAAGGTTAAAGTTATCTTGGTGTACAATAAATTTATTGTACACCTTGTGCGCACAAGATCTTTTGTTTTACAATATCAACCGCTACTTTTATCAAACACTAATATTTTACCCGCTACTTTAATAGTTATCCGTTACCCACTACTATTCACCGCTACTCGGCACTCGCTACTCTAACCACTACTCGCTTATTTTTCCAAACAAGGCGTTATTAAGTAGGAAAAGGGATTGAATGTTAATTACTTCCTCCGTAGTTTATTGAGTGGTGAGTTGATTTTATTAAACAAAGAAATAAGTGGGGTTAGTGTAGATTAAACAATTGGAGAAAAAATAGTGAAAGTATTAAAATTTATTTTTATTTTTTTGAGGGGAAGGTGAAAGTATTAAATATGACAATTCTCATTAACCTATAATAAATACGGAAAAATACAGTAATGTTGTAATGTGAATCTTGTTAGATTCATTTCAATATATTTTCGTATATTTTGGAATAATATACTTGGTACGGAACTACGGACTATATTTCAATGTAATCCAAAAAAACACACACCAAATGAGACAAAAAGAAAGGTACGAAGGAACTCAGGAAGTATTCACTTACCTAACTTACGATCGTGCTGAGATTATTAGAAGGGAATAAACATCAGAAAAAACGTTTGTTGTCCTCTCGATCTTCTCTGCCAGGTAACAATTGTTTCAATATTTCATGACTTGGGTTAATTTTTCTTCATTTCCATTCTTTCGAATTTACTTACATTTGGGTAATTAGGGTTTATATTTGATCTAATATTGTTGAATTTCTGCTAAAATTCCAATCTTTCTTCTTAGTGCTTGCTCCACATAGCCATTTAGCTTATACTTGAAATTATCTATAACTGTTAATTTTTCTGTATAATAGTTTTCTTGCTTGGAAACTAGATTGCCTCTTTAGGTTTTATCATCCGAAAACTCCAAATTCTAGGTACTAAGGTCTATTAGTGTTGATTTTTAAGTCAAAGTTGTGATATTTGACAGATTTGAGGGATCCAATTAAACAAACAAAAATGGGGGTAATTTCCGAGAAGATAGTGGAAGTGATTTCAAAATACACAGGGCTATCACCAATGTGGTTCATGATCATGGTGGGGTTGACTATAATAAGTTACAAGATAGTTAGCGGCATGTTTGTGGCGGTGAATGATTACAAAGCAGTTGAAAAGATGAAGGAAATATTCGAGGAGGATATGAGGAGGGAGGTGGTGGAGCTGGGTCAGCTGACGGAGGAGCAGCTCAAGGAATACGACGGGTCAGACCCGAAGAAGCCTCTATTGATGGCAATTAAGGGTAATATATATGATGTTTCAAGGGGTAGGATATTTTACGGGCCTGGCGGGTCATACGCTATGTTTGCTGGGAGGGATGCTACTCGGGCTTTGGCCCTCATGTCTTTTGATCCTCAGGATCTTACTGCCAACACCGACGGTCTCAGTGAATCTGAGCTTGATGTTCTTCAAGATTGGGAGCTTAAGTTTCAAGAGAAATACCCCAAGGTCGGTGTTCTTGGAGGCCAGCCGCAGCTGCAGGAGGAGGTTGTAGAAACTAAAAAAGCTGTATAGCTTACTCTTTCTAATTTCTAATTTTCTTTGTTTCATGTTTGATGATATCTCTTAATGTCAGAGAGGACCTATTATTAATCAAGTAGCTTTTGATGATATATTGATCGAACTATGTCTTATTAATCTTTCATTGTAGCTTGTTGTGGTTGACATTGCACTGATTAGCACCAACTTGTGGTTTTGAGTTTATTTGATTCTGAGTTACTCAAACTAGTCATTGCACTGATTAGCACTACCAAAATGTGATTTAGTAGTTATGTGTATATTAAGGCAACTTTGTGCGATTGGTTGAGAAGTGGACAACTTTGTGTTATTTTTGTCATATTGACAAGTTTGTGTCAATGATTGCAAATGGCCCAAAGTACATGTCCATTGATGTAATTTTCTCATAACTCCCATACTGAAGTATGCATTATAAATTGGATGTCAGTATGTCAGACACAGAGTATTATACATTTTCCTTTGTTTTTACTTTTCTCTTTTCAGCAAAATGCTTATCATTTTAGCTATTATTGTGATTTGATAATCTGCCTGGCCTTCCCAATATTTGTGGAAATTAATATAAATCTTGACTACTGTGAACATCTAAAACAGCTAGTGGTGGCCTTGATCTTAGTCGTAGGTGCTCGTTCTATTTTGATTTGAACGGGCCGAATGTTATGACTTTTAATGTGGGTCTTTTTTATGAATAAGTAGAAATCAACTTTGGTAGGGATACTAAAGAACCTTGAAGAGATTTGATCTTTTCTAGTTTGTATTGGCTGGTAAGCATTATGGTGGAAAAGTTGTGGCCTGCCCTTCAATCCTGCAAGCATCAGGTATGATTTTATTTCCTTTGTGATCCGCTCATCAATGGCAGAGTCAGATTATTAGAAAC

mRNA sequence

ATGGGGTATCTGAAAGAGGAAGGGAATAAACATCAGAAAAAACGTTTGTTGTCCTCTCGATCTTCTCTGCCAGATTTGAGGGATCCAATTAAACAAACAAAAATGGGGGTAATTTCCGAGAAGATAGTGGAAGTGATTTCAAAATACACAGGGCTATCACCAATGTGGTTCATGATCATGGTGGGGTTGACTATAATAAGTTACAAGATAGTTAGCGGCATGTTTGTGGCGGTGAATGATTACAAAGCAGTTGAAAAGATGAAGGAAATATTCGAGGAGGATATGAGGAGGGAGGTGGTGGAGCTGGGTCAGCTGACGGAGGAGCAGCTCAAGGAATACGACGGGTCAGACCCGAAGAAGCCTCTATTGATGGCAATTAAGGGTAATATATATGATGTTTCAAGGGGTAGGATATTTTACGGGCCTGGCGGGTCATACGCTATGTTTGCTGGGAGGGATGCTACTCGGGCTTTGGCCCTCATGTCTTTTGATCCTCAGGATCTTACTGCCAACACCGACGGTCTCAGTGAATCTGAGCTTGATGTTCTTCAAGATTGGGAGCTTAAGTTTCAAGAGAAATACCCCAAGGTCGGTGTTCTTGGAGGCCAGCCGCAGCTGCAGGAGGAGGTTGTAGAAACTAAAAAAGCTGTATAGCTTACTCTTTCTAATTTCTAATTTTCTTTGTTTCATGTTTGATGATATCTCTTAATGTCAGAGAGGACCTATTATTAATCAAGTAGCTTTTGATGATATATTGATCGAACTATGTCTTATTAATCTTTCATTGTAGCTTGTTGTGGTTGACATTGCACTGATTAGCACCAACTTGTGGTTTTGAGTTTATTTGATTCTGAGTTACTCAAACTAGTCATTGCACTGATTAGCACTACCAAAATGTGATTTAGTAGTTATGTGTATATTAAGGCAACTTTGTGCGATTGGTTGAGAAGTGGACAACTTTGTGTTATTTTTGTCATATTGACAAGTTTGTGTCAATGATTGCAAATGGCCCAAAGTACATGTCCATTGATGTAATTTTCTCATAACTCCCATACTGAAGTATGCATTATAAATTGGATGTCAGTATGTCAGACACAGAGTATTATACATTTTCCTTTGTTTTTACTTTTCTCTTTTCAGCAAAATGCTTATCATTTTAGCTATTATTGTGATTTGATAATCTGCCTGGCCTTCCCAATATTTGTGGAAATTAATATAAATCTTGACTACTGTGAACATCTAAAACAGCTAGTGGTGGCCTTGATCTTAGTCGTAGGTGCTCGTTCTATTTTGATTTGAACGGGCCGAATGTTATGACTTTTAATGTGGGTCTTTTTTATGAATAAGTAGAAATCAACTTTGGTAGGGATACTAAAGAACCTTGAAGAGATTTGATCTTTTCTAGTTTGTATTGGCTGGTAAGCATTATGGTGGAAAAGTTGTGGCCTGCCCTTCAATCCTGCAAGCATCAGGTATGATTTTATTTCCTTTGTGATCCGCTCATCAATGGCAGAGTCAGATTATTAGAAAC

Coding sequence (CDS)

ATGGGGTATCTGAAAGAGGAAGGGAATAAACATCAGAAAAAACGTTTGTTGTCCTCTCGATCTTCTCTGCCAGATTTGAGGGATCCAATTAAACAAACAAAAATGGGGGTAATTTCCGAGAAGATAGTGGAAGTGATTTCAAAATACACAGGGCTATCACCAATGTGGTTCATGATCATGGTGGGGTTGACTATAATAAGTTACAAGATAGTTAGCGGCATGTTTGTGGCGGTGAATGATTACAAAGCAGTTGAAAAGATGAAGGAAATATTCGAGGAGGATATGAGGAGGGAGGTGGTGGAGCTGGGTCAGCTGACGGAGGAGCAGCTCAAGGAATACGACGGGTCAGACCCGAAGAAGCCTCTATTGATGGCAATTAAGGGTAATATATATGATGTTTCAAGGGGTAGGATATTTTACGGGCCTGGCGGGTCATACGCTATGTTTGCTGGGAGGGATGCTACTCGGGCTTTGGCCCTCATGTCTTTTGATCCTCAGGATCTTACTGCCAACACCGACGGTCTCAGTGAATCTGAGCTTGATGTTCTTCAAGATTGGGAGCTTAAGTTTCAAGAGAAATACCCCAAGGTCGGTGTTCTTGGAGGCCAGCCGCAGCTGCAGGAGGAGGTTGTAGAAACTAAAAAAGCTGTATAG

Protein sequence

MGYLKEEGNKHQKKRLLSSRSSLPDLRDPIKQTKMGVISEKIVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVAVNDYKAVEKMKEIFEEDMRREVVELGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDATRALALMSFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVGVLGGQPQLQEEVVETKKAV
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo08120.1Spo08120.1mRNA


Homology
[Close]
BLAST of Spo08120.1 vs. NCBI nr
Match: gi|902106558|gb|KNA03627.1| (hypothetical protein SOVF_207300 [Spinacia oleracea])

HSP 1 Score: 356.3 bits (913), Expect = 3.800e-95
Identity = 183/183 (100.00%), Postives = 183/183 (100.00%), Query Frame = 1 

		  

Query: 35  MGVISEKIVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVAVNDYKAVEKMKEIFEED 94
           MGVISEKIVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVAVNDYKAVEKMKEIFEED
Sbjct: 1   MGVISEKIVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVAVNDYKAVEKMKEIFEED 60

Query: 95  MRREVVELGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDA 154
           MRREVVELGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDA
Sbjct: 61  MRREVVELGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDA 120

Query: 155 TRALALMSFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVGVLGGQPQLQEEVVETK 214
           TRALALMSFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVGVLGGQPQLQEEVVETK
Sbjct: 121 TRALALMSFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVGVLGGQPQLQEEVVETK 180

Query: 215 KAV 218
           KAV
Sbjct: 181 KAV 183
[Close]
BLAST of Spo08120.1 vs. NCBI nr
Match: gi|902191300|gb|KNA11814.1| (hypothetical protein SOVF_131630 [Spinacia oleracea])

HSP 1 Score: 255.4 bits (651), Expect = 9.100e-65
Identity = 131/144 (90.97%), Postives = 135/144 (93.75%), Query Frame = 1 

		  

Query: 74  MFVAVNDYKAVEKMKEIFEEDMRREVVELGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDV 133
           MFVAVNDYKAVEKMKEIFEEDMRRE VELGQLTEEQL+EYDG DPKKPLLMA+KGNIYDV
Sbjct: 1   MFVAVNDYKAVEKMKEIFEEDMRREAVELGQLTEEQLREYDGPDPKKPLLMAMKGNIYDV 60

Query: 134 SRGRIFYGPGGSYAMFAGRDATRALALMSFDPQDLTANTDGLSESELDVLQDWELKFQEK 193
           SRGRIFYGPGGSYAMF GRDATRALALMSFDPQDLTANTDGLSESELDVLQ+WELKFQE 
Sbjct: 61  SRGRIFYGPGGSYAMFDGRDATRALALMSFDPQDLTANTDGLSESELDVLQNWELKFQEN 120

Query: 194 YPKVGVLGGQPQLQEEVVETKKAV 218
            PK+GV GGQ QLQEEVVETK  V
Sbjct: 121 -PKIGVHGGQQQLQEEVVETKNVV 143
[Close]
BLAST of Spo08120.1 vs. NCBI nr
Match: gi|731316408|ref|XP_010695409.1| (PREDICTED: probable steroid-binding protein 3 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 242.7 bits (618), Expect = 6.100e-61
Identity = 130/182 (71.43%), Postives = 149/182 (81.87%), Query Frame = 1 

		  

Query: 42  IVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVAVNDYKAVEKMKEIFEEDMRREVVE 101
           IVE I  YTGLSP+   I+  LTII+YKIV  MFVAVNDYK VE+MKEI+E+DM+RE V+
Sbjct: 12  IVEAIKNYTGLSPLSLTIITVLTIITYKIVCTMFVAVNDYKEVEEMKEIYEQDMKREPVQ 71

Query: 102 LGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDATRALALM 161
           LG++TEE+L++YDGSD KKPLLMAIKG I+DVSRGR+FYG  G Y+MFAGRDATRALALM
Sbjct: 72  LGEVTEEELRKYDGSDLKKPLLMAIKGQIFDVSRGRMFYGRDGPYSMFAGRDATRALALM 131

Query: 162 SFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVGVLGGQPQLQE------EVVETKK 218
           SFD QDLT N DGLSESELDVLQDWELKF EKYPKVGVL  +    E      E++ETKK
Sbjct: 132 SFDVQDLTGNIDGLSESELDVLQDWELKFLEKYPKVGVLVYKENSAEAETETDELLETKK 191
[Close]
BLAST of Spo08120.1 vs. NCBI nr
Match: gi|747048057|ref|XP_011070022.1| (PREDICTED: membrane steroid-binding protein 2-like [Sesamum indicum])

HSP 1 Score: 203.4 bits (516), Expect = 4.100e-49
Identity = 103/161 (63.98%), Postives = 124/161 (77.02%), Query Frame = 1 

		  

Query: 40  EKIVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVAVNDYKAVEKMKEIFEEDMRREV 99
           E + E I+ YTGLSP  F  ++ L  ++YK+V GMFVA +DY AV++  E       RE 
Sbjct: 5   ETLTESINHYTGLSPAAFFTILALMYVTYKVVCGMFVAADDYAAVKRANEF----ALREP 64

Query: 100 VELGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDATRALA 159
           V++G +TEE+L+ YDGSDP KPLLMAIKG IYDVSR R+FYGPGG YA+FAGRDA+RALA
Sbjct: 65  VQMGDVTEEELRAYDGSDPSKPLLMAIKGQIYDVSRSRMFYGPGGPYALFAGRDASRALA 124

Query: 160 LMSFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVGVL 201
           LMSFDP+DLT N +GLS SEL+VLQDWE KF EKY KVG L
Sbjct: 125 LMSFDPKDLTGNIEGLSASELEVLQDWEYKFMEKYVKVGQL 161
[Close]
BLAST of Spo08120.1 vs. NCBI nr
Match: gi|1021034218|gb|KZM92002.1| (hypothetical protein DCAR_020633 [Daucus carota subsp. sativus])

HSP 1 Score: 194.9 bits (494), Expect = 1.500e-46
Identity = 100/174 (57.47%), Postives = 125/174 (71.84%), Query Frame = 1 

		  

Query: 42  IVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVAVNDYKAVEKMKEIFEEDMRREVVE 101
           + + IS YTGLSP  F  ++ L  + YK+V GMFVA +DY+AV++  +     + RE V+
Sbjct: 7   VTDTISYYTGLSPAAFFTILALMYVVYKVVCGMFVAADDYRAVKRSNDF----VLREPVQ 66

Query: 102 LGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDATRALALM 161
           LG +T E+L+ YDGSDP KPLLMAIKG IYDVSR R+FYGPGG YA+FAGRDA+RALALM
Sbjct: 67  LGDVTVEELRAYDGSDPNKPLLMAIKGQIYDVSRSRMFYGPGGPYALFAGRDASRALALM 126

Query: 162 SFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVGVLGGQPQLQEEVVETKK 216
           SF+ +DLT N DGLS  EL+VLQDWE KF EKY KVG +  +    E   +  K
Sbjct: 127 SFESKDLTGNIDGLSPDELEVLQDWEYKFMEKYMKVGQIVSEKSKAEHTEDEDK 176
[Close]
BLAST of Spo08120.1 vs. UniProtKB/TrEMBL
Match: A0A0K9Q8N3_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_207300 PE=3 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 2.600e-95
Identity = 183/183 (100.00%), Postives = 183/183 (100.00%), Query Frame = 1 

		  

Query: 35  MGVISEKIVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVAVNDYKAVEKMKEIFEED 94
           MGVISEKIVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVAVNDYKAVEKMKEIFEED
Sbjct: 1   MGVISEKIVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVAVNDYKAVEKMKEIFEED 60

Query: 95  MRREVVELGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDA 154
           MRREVVELGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDA
Sbjct: 61  MRREVVELGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDA 120

Query: 155 TRALALMSFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVGVLGGQPQLQEEVVETK 214
           TRALALMSFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVGVLGGQPQLQEEVVETK
Sbjct: 121 TRALALMSFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVGVLGGQPQLQEEVVETK 180

Query: 215 KAV 218
           KAV
Sbjct: 181 KAV 183
[Close]
BLAST of Spo08120.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QX08_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_131630 PE=3 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 6.400e-65
Identity = 131/144 (90.97%), Postives = 135/144 (93.75%), Query Frame = 1 

		  

Query: 74  MFVAVNDYKAVEKMKEIFEEDMRREVVELGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDV 133
           MFVAVNDYKAVEKMKEIFEEDMRRE VELGQLTEEQL+EYDG DPKKPLLMA+KGNIYDV
Sbjct: 1   MFVAVNDYKAVEKMKEIFEEDMRREAVELGQLTEEQLREYDGPDPKKPLLMAMKGNIYDV 60

Query: 134 SRGRIFYGPGGSYAMFAGRDATRALALMSFDPQDLTANTDGLSESELDVLQDWELKFQEK 193
           SRGRIFYGPGGSYAMF GRDATRALALMSFDPQDLTANTDGLSESELDVLQ+WELKFQE 
Sbjct: 61  SRGRIFYGPGGSYAMFDGRDATRALALMSFDPQDLTANTDGLSESELDVLQNWELKFQEN 120

Query: 194 YPKVGVLGGQPQLQEEVVETKKAV 218
            PK+GV GGQ QLQEEVVETK  V
Sbjct: 121 -PKIGVHGGQQQLQEEVVETKNVV 143
[Close]
BLAST of Spo08120.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CZ14_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g029570 PE=3 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 4.300e-61
Identity = 130/182 (71.43%), Postives = 149/182 (81.87%), Query Frame = 1 

		  

Query: 42  IVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVAVNDYKAVEKMKEIFEEDMRREVVE 101
           IVE I  YTGLSP+   I+  LTII+YKIV  MFVAVNDYK VE+MKEI+E+DM+RE V+
Sbjct: 12  IVEAIKNYTGLSPLSLTIITVLTIITYKIVCTMFVAVNDYKEVEEMKEIYEQDMKREPVQ 71

Query: 102 LGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDATRALALM 161
           LG++TEE+L++YDGSD KKPLLMAIKG I+DVSRGR+FYG  G Y+MFAGRDATRALALM
Sbjct: 72  LGEVTEEELRKYDGSDLKKPLLMAIKGQIFDVSRGRMFYGRDGPYSMFAGRDATRALALM 131

Query: 162 SFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVGVLGGQPQLQE------EVVETKK 218
           SFD QDLT N DGLSESELDVLQDWELKF EKYPKVGVL  +    E      E++ETKK
Sbjct: 132 SFDVQDLTGNIDGLSESELDVLQDWELKFLEKYPKVGVLVYKENSAEAETETDELLETKK 191
[Close]
BLAST of Spo08120.1 vs. UniProtKB/TrEMBL
Match: A0A161ZVE4_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_020633 PE=4 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 1.000e-46
Identity = 100/174 (57.47%), Postives = 125/174 (71.84%), Query Frame = 1 

		  

Query: 42  IVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVAVNDYKAVEKMKEIFEEDMRREVVE 101
           + + IS YTGLSP  F  ++ L  + YK+V GMFVA +DY+AV++  +     + RE V+
Sbjct: 7   VTDTISYYTGLSPAAFFTILALMYVVYKVVCGMFVAADDYRAVKRSNDF----VLREPVQ 66

Query: 102 LGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDATRALALM 161
           LG +T E+L+ YDGSDP KPLLMAIKG IYDVSR R+FYGPGG YA+FAGRDA+RALALM
Sbjct: 67  LGDVTVEELRAYDGSDPNKPLLMAIKGQIYDVSRSRMFYGPGGPYALFAGRDASRALALM 126

Query: 162 SFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVGVLGGQPQLQEEVVETKK 216
           SF+ +DLT N DGLS  EL+VLQDWE KF EKY KVG +  +    E   +  K
Sbjct: 127 SFESKDLTGNIDGLSPDELEVLQDWEYKFMEKYMKVGQIVSEKSKAEHTEDEDK 176
[Close]
BLAST of Spo08120.1 vs. UniProtKB/TrEMBL
Match: A0A103XQH7_CYNCS (Cytochrome b5-like heme/steroid binding domain-containing protein OS=Cynara cardunculus var. scolymus GN=Ccrd_002882 PE=3 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 8.600e-46
Identity = 94/159 (59.12%), Postives = 123/159 (77.36%), Query Frame = 1 

		  

Query: 42  IVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVAVNDYKAVEKMKEIFEEDMRREVVE 101
           + + I++YTGLSP  F  +  + +++YK+V+ MFVA +DY AV++   I      RE V+
Sbjct: 7   VTDAINQYTGLSPAAFFTISAMMVVAYKVVTTMFVAADDYVAVKREHNI----SLREPVQ 66

Query: 102 LGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDATRALALM 161
           +G +TEE+L+ Y+GSDP KPLLMAIKG IYDVSR R+FYGPGG YA+FAGRDA+RALAL+
Sbjct: 67  VGSITEEELRPYNGSDPNKPLLMAIKGKIYDVSRSRMFYGPGGPYALFAGRDASRALALL 126

Query: 162 SFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVGVL 201
           SF+P DLT N DGL  S+L+VL+DWE KF+EKY KVG L
Sbjct: 127 SFEPSDLTGNIDGLGASDLEVLEDWEAKFEEKYVKVGQL 161
[Close]
BLAST of Spo08120.1 vs. ExPASy Swiss-Prot
Match: MSBP2_ARATH (Membrane steroid-binding protein 2 OS=Arabidopsis thaliana GN=MSBP2 PE=1 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 7.800e-40
Identity = 92/182 (50.55%), Postives = 120/182 (65.93%), Query Frame = 1 

		  

Query: 38  ISEKIVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVAVNDYKAVEKMKEIFEEDMRR 97
           I E + E I+ YTGLSP  F  ++ L    Y++VSG FV+   ++    ++   + +   
Sbjct: 5   IWETLKETITAYTGLSPAAFFTVLALAFAVYQVVSGFFVSPEVHRP-RSLEVQPQSEPLP 64

Query: 98  EVVELGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDATRA 157
             V+LG++TEE+LK YDGSD KKPLLMAIKG IYDVS+ R+FYGPGG YA+FAG+DA+RA
Sbjct: 65  PPVQLGEITEEELKLYDGSDSKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRA 124

Query: 158 LALMSFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVGVL---GGQPQLQEEVVETK 217
           LA MSF+ QDLT +  GL   EL+ LQDWE KF  KY KVG +    G+ +   E  E K
Sbjct: 125 LAKMSFEDQDLTGDISGLGAFELEALQDWEYKFMSKYVKVGTIQKKDGEGKESSEPSEAK 184
[Close]
BLAST of Spo08120.1 vs. ExPASy Swiss-Prot
Match: MSBP1_ARATH (Membrane steroid-binding protein 1 OS=Arabidopsis thaliana GN=MSBP1 PE=1 SV=2)

HSP 1 Score: 163.3 bits (412), Expect = 3.000e-39
Identity = 87/173 (50.29%), Postives = 118/173 (68.21%), Query Frame = 1 

		  

Query: 40  EKIVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVA----VNDYKAVEKMKEIFEEDM 99
           + + E I  YTGLSP+ F   + L    Y+++SG F +    VN ++    + +  EE  
Sbjct: 7   QTLKEAIHAYTGLSPVVFFTALALAFAIYQVISGWFASPFDDVNRHQRARSLAQE-EEPP 66

Query: 100 RREVVELGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDAT 159
             + V++G++TEE+LK+YDGSDP+KPLLMAIK  IYDV++ R+FYGPGG YA+FAG+DA+
Sbjct: 67  IPQPVQVGEITEEELKQYDGSDPQKPLLMAIKHQIYDVTQSRMFYGPGGPYALFAGKDAS 126

Query: 160 RALALMSFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVG---VLGGQPQ 206
           RALA MSF+ +DLT +  GL   ELD LQDWE KF  KY KVG   V G +P+
Sbjct: 127 RALAKMSFEEKDLTWDVSGLGPFELDALQDWEYKFMSKYAKVGTVKVAGSEPE 178
[Close]
BLAST of Spo08120.1 vs. ExPASy Swiss-Prot
Match: SBP3_ARATH (Probable steroid-binding protein 3 OS=Arabidopsis thaliana GN=MP3 PE=1 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 2.100e-24
Identity = 49/95 (51.58%), Postives = 72/95 (75.79%), Query Frame = 1 

		  

Query: 104 QLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDATRALALMSF 163
           + T EQL +Y+G+D  KP+ +AIKG ++DV+ G+ FYG GG Y+MFAG+DA+RAL  MS 
Sbjct: 2   EFTAEQLSQYNGTDESKPIYVAIKGRVFDVTTGKSFYGSGGDYSMFAGKDASRALGKMSK 61

Query: 164 DPQDLTANTDGLSESELDVLQDWELKFQEKYPKVG 199
           + +D++ + +GL+E E++ L DWE KF+ KYP VG
Sbjct: 62  NEEDVSPSLEGLTEKEINTLNDWETKFEAKYPVVG 96
[Close]
BLAST of Spo08120.1 vs. ExPASy Swiss-Prot
Match: NEUFC_CAEBR (Neuferricin homolog OS=Caenorhabditis briggsae GN=tag-131 PE=3 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 3.200e-17
Identity = 52/120 (43.33%), Postives = 68/120 (56.67%), Query Frame = 1 

		  

Query: 106 TEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDATRALALMSFDP 165
           T EQL  +DGS   KP  +AI G +YDV   + +YGPG SY  FAGRDATRA     F  
Sbjct: 102 TPEQLHFFDGSRDSKPCYLAILGRVYDVDGKKEYYGPGKSYHHFAGRDATRAFTTGDFTE 161

Query: 166 QDLTANTDGLSESELDVLQDWELKFQEKYPKVGVLG-------GQP--QLQEEVVETKKA 217
             L A+T GLS  EL  ++DW   + ++YP VGV+        GQP  +L + +   +KA
Sbjct: 162 NGLVASTHGLSHDELLSIRDWVSFYDKEYPLVGVVADLYYDSDGQPTAELTDVLARVEKA 221
[Close]
BLAST of Spo08120.1 vs. ExPASy Swiss-Prot
Match: NENF_BOVIN (Neudesin OS=Bos taurus GN=NENF PE=2 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.200e-16
Identity = 43/94 (45.74%), Postives = 61/94 (64.89%), Query Frame = 1 

		  

Query: 106 TEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDATRALALMSFDP 165
           TEE+L  Y G +  +P+ MA+KG ++DV+ G+ FYG G  Y    G+D+TR +A MS DP
Sbjct: 45  TEEELARYGGEEEDQPIYMAVKGVVFDVTSGKEFYGRGAPYNALTGKDSTRGVAKMSLDP 104

Query: 166 QDLTANTDGLSESELDVLQD-WELKFQEKYPKVG 199
            DLT +T GL+  EL+ L D +   ++ KYP VG
Sbjct: 105 ADLTHDTTGLTAEELESLDDVFTRVYKAKYPIVG 138
[Close]
BLAST of Spo08120.1 vs. TAIR (Arabidopsis)
Match: AT3G48890.1 (membrane-associated progesterone binding protein 3)

HSP 1 Score: 165.2 bits (417), Expect = 4.400e-41
Identity = 92/182 (50.55%), Postives = 120/182 (65.93%), Query Frame = 1 

		  

Query: 38  ISEKIVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVAVNDYKAVEKMKEIFEEDMRR 97
           I E + E I+ YTGLSP  F  ++ L    Y++VSG FV+   ++    ++   + +   
Sbjct: 5   IWETLKETITAYTGLSPAAFFTVLALAFAVYQVVSGFFVSPEVHRP-RSLEVQPQSEPLP 64

Query: 98  EVVELGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDATRA 157
             V+LG++TEE+LK YDGSD KKPLLMAIKG IYDVS+ R+FYGPGG YA+FAG+DA+RA
Sbjct: 65  PPVQLGEITEEELKLYDGSDSKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRA 124

Query: 158 LALMSFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVGVL---GGQPQLQEEVVETK 217
           LA MSF+ QDLT +  GL   EL+ LQDWE KF  KY KVG +    G+ +   E  E K
Sbjct: 125 LAKMSFEDQDLTGDISGLGAFELEALQDWEYKFMSKYVKVGTIQKKDGEGKESSEPSEAK 184
[Close]
BLAST of Spo08120.1 vs. TAIR (Arabidopsis)
Match: AT5G52240.1 (membrane steroid binding protein 1)

HSP 1 Score: 163.3 bits (412), Expect = 1.700e-40
Identity = 87/173 (50.29%), Postives = 118/173 (68.21%), Query Frame = 1 

		  

Query: 40  EKIVEVISKYTGLSPMWFMIMVGLTIISYKIVSGMFVA----VNDYKAVEKMKEIFEEDM 99
           + + E I  YTGLSP+ F   + L    Y+++SG F +    VN ++    + +  EE  
Sbjct: 7   QTLKEAIHAYTGLSPVVFFTALALAFAIYQVISGWFASPFDDVNRHQRARSLAQE-EEPP 66

Query: 100 RREVVELGQLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDAT 159
             + V++G++TEE+LK+YDGSDP+KPLLMAIK  IYDV++ R+FYGPGG YA+FAG+DA+
Sbjct: 67  IPQPVQVGEITEEELKQYDGSDPQKPLLMAIKHQIYDVTQSRMFYGPGGPYALFAGKDAS 126

Query: 160 RALALMSFDPQDLTANTDGLSESELDVLQDWELKFQEKYPKVG---VLGGQPQ 206
           RALA MSF+ +DLT +  GL   ELD LQDWE KF  KY KVG   V G +P+
Sbjct: 127 RALAKMSFEEKDLTWDVSGLGPFELDALQDWEYKFMSKYAKVGTVKVAGSEPE 178
[Close]
BLAST of Spo08120.1 vs. TAIR (Arabidopsis)
Match: AT2G24940.1 (membrane-associated progesterone binding protein 2)

HSP 1 Score: 114.0 bits (284), Expect = 1.200e-25
Identity = 49/95 (51.58%), Postives = 72/95 (75.79%), Query Frame = 1 

		  

Query: 104 QLTEEQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDATRALALMSF 163
           + T EQL +Y+G+D  KP+ +AIKG ++DV+ G+ FYG GG Y+MFAG+DA+RAL  MS 
Sbjct: 2   EFTAEQLSQYNGTDESKPIYVAIKGRVFDVTTGKSFYGSGGDYSMFAGKDASRALGKMSK 61

Query: 164 DPQDLTANTDGLSESELDVLQDWELKFQEKYPKVG 199
           + +D++ + +GL+E E++ L DWE KF+ KYP VG
Sbjct: 62  NEEDVSPSLEGLTEKEINTLNDWETKFEAKYPVVG 96
[Close]
BLAST of Spo08120.1 vs. TAIR (Arabidopsis)
Match: AT4G14965.1 (membrane-associated progesterone binding protein 4)

HSP 1 Score: 82.0 bits (201), Expect = 4.900e-16
Identity = 40/96 (41.67%), Postives = 58/96 (60.42%), Query Frame = 1 

		  

Query: 108 EQLKEYDGSDPKKPLLMAIKGNIYDVSRGRIFYGPGGSYAMFAGRDATRALALMSFDPQD 167
           E+L  Y+G+D   P+L+ I G+++DV++G+  YG GG Y  FAGRDA+RA    +F    
Sbjct: 45  EELALYNGTDETLPILLGILGSVFDVTKGKFHYGSGGGYNHFAGRDASRAFVSGNFTGDG 104

Query: 168 LTANTDGLSESELDVLQDWELKFQEKYPKVGVLGGQ 204
           LT +  GLS SE+  + DW   +   Y  VG L G+
Sbjct: 105 LTDSLQGLSSSEVKSIVDWRGFYSRTYTPVGKLVGR 140
The following BLAST results are available for this feature:
BLAST of Spo08120.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902106558|gb|KNA03627.1|3.8e-95100.hypothetical protein SOVF_2073... [more]
gi|902191300|gb|KNA11814.1|9.1e-6590.9hypothetical protein SOVF_1316... [more]
gi|731316408|ref|XP_010695409.1|6.1e-6171.4PREDICTED: probable steroid-bi... [more]
gi|747048057|ref|XP_011070022.1|4.1e-4963.9PREDICTED: membrane steroid-bi... [more]
gi|1021034218|gb|KZM92002.1|1.5e-4657.4hypothetical protein DCAR_0206... [more]
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BLAST of Spo08120.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9Q8N3_SPIOL2.6e-95100.Uncharacterized protein OS=Spi... [more]
A0A0K9QX08_SPIOL6.4e-6590.9Uncharacterized protein OS=Spi... [more]
A0A0J8CZ14_BETVU4.3e-6171.4Uncharacterized protein OS=Bet... [more]
A0A161ZVE4_DAUCA1.0e-4657.4Uncharacterized protein OS=Dau... [more]
A0A103XQH7_CYNCS8.6e-4659.1Cytochrome b5-like heme/steroi... [more]
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BLAST of Spo08120.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5
Match NameE-valueIdentityDescription
MSBP2_ARATH7.8e-4050.5Membrane steroid-binding prote... [more]
MSBP1_ARATH3.0e-3950.2Membrane steroid-binding prote... [more]
SBP3_ARATH2.1e-2451.5Probable steroid-binding prote... [more]
NEUFC_CAEBR3.2e-1743.3Neuferricin homolog OS=Caenorh... [more]
NENF_BOVIN1.2e-1645.7Neudesin OS=Bos taurus GN=NENF... [more]
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BLAST of Spo08120.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 4
Match NameE-valueIdentityDescription
AT3G48890.14.4e-4150.5membrane-associated progestero... [more]
AT5G52240.11.7e-4050.2membrane steroid binding prote... [more]
AT2G24940.11.2e-2551.5membrane-associated progestero... [more]
AT4G14965.14.9e-1641.6membrane-associated progestero... [more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001199Cytochrome b5-like heme/steroid binding domainGENE3D3.10.120.10coord: 104..200
score: 1.7
IPR001199Cytochrome b5-like heme/steroid binding domainPFAMPF00173Cyt-b5coord: 106..200
score: 2.5
IPR001199Cytochrome b5-like heme/steroid binding domainSMARTSM01117Cyt_b5_2coord: 105..201
score: 7.7
IPR001199Cytochrome b5-like heme/steroid binding domainunknownSSF55856Cytochrome b5-like heme/steroid binding domaincoord: 101..200
score: 1.13
NoneNo IPR availablePANTHERPTHR10281MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATEDcoord: 35..200
score: 4.7
NoneNo IPR availablePANTHERPTHR10281:SF40SUBFAMILY NOT NAMEDcoord: 35..200
score: 4.7

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000394 RNA splicing, via endonucleolytic cleavage and ligation
biological_process GO:0046777 protein autophosphorylation
biological_process GO:0009069 serine family amino acid metabolic process
biological_process GO:0006508 proteolysis
biological_process GO:0006366 transcription by RNA polymerase II
biological_process GO:0006144 purine nucleobase metabolic process
biological_process GO:0006206 pyrimidine nucleobase metabolic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0005730 nucleolus
cellular_component GO:0005665 DNA-directed RNA polymerase II, core complex
molecular_function GO:0020037 heme binding
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity
molecular_function GO:0005516 calmodulin binding
molecular_function GO:0004683 calmodulin-dependent protein kinase activity
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0005524 ATP binding
molecular_function GO:0004190 aspartic-type endopeptidase activity
molecular_function GO:0003723 RNA binding
RNA-Seq Expression