Query: 61 IAVGSSLGALFYPVSPNHDCDVNDNVSSSSS-----SSVFDYFKGKSMLTFADWSSAPTE 120 IA+GSS GALFYP S + + N NVSSSSS SSVFD +GKSML FADWS PTE Sbjct: 61 IAIGSSFGALFYPFSSDDSINNNHNVSSSSSSSSSLSSVFDSIRGKSMLAFADWSPGPTE 120
Query: 121 ATSISSLDSVPRSLSLKNSRFLFPDAFRRKVFFKYEKRMRMRSPPEKVFEYFASVQSSSG 180 A S +SLDS P SLSLKNSRFLFPDA+RRKVFFKYEKR+RMRSPPEKVFEYFASV S G Sbjct: 121 AAS-TSLDSAPNSLSLKNSRFLFPDAYRRKVFFKYEKRIRMRSPPEKVFEYFASVLKSDG 180
Query: 301 GFKLGGSVENGGLLEYFFGETGEMQLHYDRFVRFLRKLHDEIVRLEFSHYDYKSQGTISA 360 G LG SVENGGLLEYFFGE GEMQLH+D+FVRFLRKLHDEIVRLEFSHYDY+SQGTI A Sbjct: 301 GINLGDSVENGGLLEYFFGEDGEMQLHHDKFVRFLRKLHDEIVRLEFSHYDYRSQGTILA 360
Query: 61 IAVGSSLGALFYPVSPNHDCDVNDNVSSSSSSS-----VFDYFKGKSMLTFADWSSAPTE 120 IA+GSS GALFYP S + + N NVSSSSSSS VFD +GKSML FADWS PTE Sbjct: 61 IAIGSSFGALFYPFSSDDSINNNHNVSSSSSSSSSLSSVFDSIRGKSMLAFADWSPGPTE 120
Query: 121 ATSISSLDSVPRSLSLKNSRFLFPDAFRRKVFFKYEKRMRMRSPPEKVFEYFASVQSSSG 180 A S +SLDS P SLSLKNSRFLFPDA+RRKVFFKYEKR+RMRSPPEKVFEYFASV S G Sbjct: 121 AAS-TSLDSAPNSLSLKNSRFLFPDAYRRKVFFKYEKRIRMRSPPEKVFEYFASVLKSDG 180
Query: 301 GFKLGGSVENGGLLEYFFGETGEMQLHYDRFVRFLRKLHDEIVRLEFSHYDYKSQGTISA 360 G LG SVENGGLLEYFFGE GEMQLH+D+FVRFLRKLHDEIVRLEFSHYDY+SQGTI A Sbjct: 301 GINLGDSVENGGLLEYFFGEDGEMQLHHDKFVRFLRKLHDEIVRLEFSHYDYRSQGTILA 360
BLAST of Spo09581.1 vs. NCBI nr Match: gi|802626965|ref|XP_012076491.1| (PREDICTED: calcium uptake protein 1, mitochondrial isoform X1 [Jatropha curcas])
Query: 7 LRTAKSSINLIPTTQRLPVRHLSTAASGGAIQGDPQPSSS-------ETNPRSWVLKLVS 66 LR ++SSI + QRL R S A +SS E + ++ S Sbjct: 7 LRRSRSSIYQLYLKQRLQSRQYSNIAPSSTSSLSLTCASSLKANNENENHTYGSFMRWFS 66
Query: 67 GIAVGSSLGALFYPVSPNHDCDVNDNVSSSSSSSVFDYFKGKSMLTFADWSSAPTEATSI 126 GIA+GSSLG + ++SSS F +S+L+FADW A +++ Sbjct: 67 GIALGSSLGLFCW--------------YTNSSSDAESAFLNRSLLSFADWPMATAVKSAV 126
BLAST of Spo09581.1 vs. NCBI nr Match: gi|823259662|ref|XP_012462545.1| (PREDICTED: calcium uptake protein 1, mitochondrial-like isoform X1 [Gossypium raimondii])
Query: 4 SSILRTAKSSINLIPTTQRLPVRHLSTAASGGAIQGDPQPSSSETNPRSWVLKLVSGIAV 63 +S+ R++ S ++L QRL S+ A DP + ++ R++V K VSG+AV Sbjct: 5 TSLKRSSPSMLHLSLIQQRLLTCQFSSLPLTDASNIDPN-NKRDSFSRTFV-KWVSGLAV 64
Query: 64 GSSLGALFYPVSPNHDCDVNDNVSSSSSSSVFDYFKGKSMLTFADWSSAPTEATSISSLD 123 GSSLGA+++ S + VS S+ +FK K L+F++WS E+T+ S Sbjct: 65 GSSLGAVYWWYSTS--------VSDWGSA----FFK-KPFLSFSEWSMESDESTTDGS-K 124
Query: 124 SVPRSLSLKN--SRFLFPDAFRRKVFFKYEKRMRMRSPPEKVFEYFASVQSSSGEILMTP 183 +V L+L + S+F+F + +RRKVFF YEKR+R+RSPPEKVFEYFAS Q+ GE+ M P Sbjct: 125 TVFHKLALPDYSSKFIFGEVYRRKVFFNYEKRLRLRSPPEKVFEYFASFQTPEGELFMRP 184
Query: 61 IAVGSSLGALFYPVSPNHDCDVNDNVSSSSS-----SSVFDYFKGKSMLTFADWSSAPTE 120 IA+GSS GALFYP S + + N NVSSSSS SSVFD +GKSML FADWS PTE Sbjct: 61 IAIGSSFGALFYPFSSDDSINNNHNVSSSSSSSSSLSSVFDSIRGKSMLAFADWSPGPTE 120
Query: 121 ATSISSLDSVPRSLSLKNSRFLFPDAFRRKVFFKYEKRMRMRSPPEKVFEYFASVQSSSG 180 A S +SLDS P SLSLKNSRFLFPDA+RRKVFFKYEKR+RMRSPPEKVFEYFASV S G Sbjct: 121 AAS-TSLDSAPNSLSLKNSRFLFPDAYRRKVFFKYEKRIRMRSPPEKVFEYFASVLKSDG 180
Query: 301 GFKLGGSVENGGLLEYFFGETGEMQLHYDRFVRFLRKLHDEIVRLEFSHYDYKSQGTISA 360 G LG SVENGGLLEYFFGE GEMQLH+D+FVRFLRKLHDEIVRLEFSHYDY+SQGTI A Sbjct: 301 GINLGDSVENGGLLEYFFGEDGEMQLHHDKFVRFLRKLHDEIVRLEFSHYDYRSQGTILA 360
Query: 7 LRTAKSSINLIPTTQRLPVRHLSTAASGGAIQGDPQPSSS-------ETNPRSWVLKLVS 66 LR ++SSI + QRL R S A +SS E + ++ S Sbjct: 7 LRRSRSSIYQLYLKQRLQSRQYSNIAPSSTSSLSLTCASSLKANNENENHTYGSFMRWFS 66
Query: 67 GIAVGSSLGALFYPVSPNHDCDVNDNVSSSSSSSVFDYFKGKSMLTFADWSSAPTEATSI 126 GIA+GSSLG + ++SSS F +S+L+FADW A +++ Sbjct: 67 GIALGSSLGLFCW--------------YTNSSSDAESAFLNRSLLSFADWPMATAVKSAV 126
Query: 4 SSILRTAKSSINLIPTTQRLPVRHLSTAASGGAIQGDPQPSSSETNPRSWVLKLVSGIAV 63 +S+ R++ S ++L QRL S+ A DP + ++ R++V K VSG+AV Sbjct: 5 TSLKRSSPSMLHLSLIQQRLLTCQFSSLPLTDASNIDPN-NKRDSFSRTFV-KWVSGLAV 64
Query: 64 GSSLGALFYPVSPNHDCDVNDNVSSSSSSSVFDYFKGKSMLTFADWSSAPTEATSISSLD 123 GSSLGA+++ S + VS S+ +FK K L+F++WS E+T+ S Sbjct: 65 GSSLGAVYWWYSTS--------VSDWGSA----FFK-KPFLSFSEWSMESDESTTDGS-K 124
Query: 124 SVPRSLSLKN--SRFLFPDAFRRKVFFKYEKRMRMRSPPEKVFEYFASVQSSSGEILMTP 183 +V L+L + S+F+F + +RRKVFF YEKR+R+RSPPEKVFEYFAS Q+ GE+ M P Sbjct: 125 TVFHKLALPDYSSKFIFGEVYRRKVFFNYEKRLRLRSPPEKVFEYFASFQTPEGELFMRP 184
Query: 2 SSSSILRTAKSSINLIPTTQRLPVRHLSTAA--SGGAIQGDPQPSSSETNPRSWVLKLVS 61 S +++ R++ S+ L QRL S+ + I D + S ++ W+ S Sbjct: 3 SLTALRRSSPSADRLFLIQQRLRTCQFSSLPPINASKIDSDDRKDSVASSFVKWI----S 62
Query: 62 GIAVGSSLGALFYPVSPNHDCDVNDNVSSSSSSSVFDYFKGKSMLTFADWSSAPTEATSI 121 GIAVGSSLG +++ S+S S F K +FA+WS+A E+T Sbjct: 63 GIAVGSSLGLVYW--------------YSTSGSDWRSAFLKKPFWSFAEWSTATAESTVD 122
Query: 122 SSLDSVPRSLSLKN--SRFLFPDAFRRKVFFKYEKRMRMRSPPEKVFEYFASVQSSSGEI 181 S ++ R L+L + S+F+F + RRKVFF YEKR+R+RSPPEKVF YFAS ++ GE+ Sbjct: 123 GSRNAF-RKLALPDYSSKFIFGEVHRRKVFFNYEKRLRLRSPPEKVFVYFASFRTPEGEL 182
Query: 262 KMFDLDKNGEIDRDEFKKVMALMRNQNRQGSTHRDGLRHGFKLGGSVENGGLLEYFFGET 321 +MFDL+ +G++D +EF+ V L+R Q G+ HRD G G N L+ YFFG Sbjct: 278 RMFDLNGDGDVDCEEFEMVATLVRQQTSMGTRHRDHANTGNTFKG--VNSALITYFFGPN 337
Query: 322 GEMQLHYDRFVRFLRKLHDEIVRLEFSHYDYKSQGTISAKDFALSMVASA--DLNHVNKL 381 + +L ++F+ F +L EI+ LEF + +G I+ DFA ++A A L K Sbjct: 338 MDEKLTIEKFLDFQEQLQREILSLEFERKEPNDEGNITEADFAELLLAYAGYPLKKKQKK 397
Query: 382 LERVDELDNCPELSSTRISEDQFCKFAELRTKLEPFSLALLAYGKVNGLLTRKDFQRAAS 441 L+RV IS+ + F + AL Y + ++ Q A Sbjct: 398 LKRVKRRFRD---HGKGISKQDYLDFFHFLNNINDVDTALTFYHIAGASIDQQTLQHVAK 457
Query: 442 QVCGVDLNDNVVDIVFHVFDANRDGNLCAEEFLRVLQKRA-RDIAQPNETGILGFLSCCR 497 V V+L+D+VVD+VF +FD N D L +EF+ V++ R R + +P +TG L + Sbjct: 458 TVAMVNLSDHVVDVVFTIFDENNDNQLSNKEFISVMKNRVQRGLEKPKDTGFLKMMRSVF 515
Query: 142 FRRKVFFKYEKRMRMRSPPEKVFEYFASVQ-------SSSGEILMTPSDLMRAVVP-VFP 201 FR + +YE R+R+ S P+K+F YFA+++ S E+ MTP D +R+ P V Sbjct: 160 FRERRIIEYEDRLRLYSTPDKIFRYFATLKIIDPNEDSGRFEVFMTPEDFLRSFTPGVMQ 219
Query: 202 PSESGLVR-DGYLSGERCPGELRCPASEFFMLFDVNNDGLISFKEYIFFVTLLSIPESSF 261 P GL Y + + P S F+ L +GLI+F +Y+F +TLLS + F Sbjct: 220 PRRWGLDSFKNYNPEKHKRHKFSDPDSIFYKL---GENGLINFSDYLFLMTLLSTSHADF 279
Query: 262 SVAFKMFDLDKNGEIDRDEFKKVMALMRNQNRQGSTHRDGL--RHGFKLGGSVENGGLLE 321 ++AFK+FD+D NG +D++EF KV L+ +Q G HRD + F++ N L Sbjct: 280 ALAFKIFDVDGNGALDKEEFTKVQQLIMSQTTVGQRHRDHITPNQSFRV---ETNSALET 339
Query: 322 YFFGETGEMQLHYDRFVRFLRKLHDEIVRLEFSHYDY--KSQGTISAKDFALSMVASADL 381 YFFG+ G+ L ++F+ F +L +I+++EF D G I+ FA ++ A + Sbjct: 340 YFFGKDGKGSLSSEKFIEFQERLQHDILKMEFERRDALDNPDGLINEDSFAQLLLLHAQI 399
Query: 382 NHVNK--LLERVDELDNCPELSSTRISEDQFCKFAELRTKLEPFSLALLAYGKVNGLLTR 441 N + +L+RV L E + F E ++ +AL + + Sbjct: 400 NEKKQKHMLKRVKRRFKGENLKGISFGETK--AFFEFLYHIDDVDIALHFHKMAGMSIDA 459
Query: 442 KDFQRAASQVCGVDLNDNVVDIVFHVFDANRDGNLCAEEFLRVLQKR-ARDIAQPNETGI 496 K QR A +V G+ L+D+VVD+V +FD N DG L EE + V+++R R + +P +TG+ Sbjct: 460 KLLQRVAVKVTGIPLSDHVVDVVITLFDDNLDGKLSHEEMVAVMRRRMRRGLERPRDTGL 519
Query: 142 FRRKVFFKYEKRMRMRSPPEKVFEYFASVQ-----SSSG---EILMTPSDLMRAVVP-VF 201 FR + +YE R+R+ S P+K+F YFA+++ SG E+ MTP D +R+ P V Sbjct: 158 FRERRIIEYEDRLRLYSTPDKIFRYFATLKIIDPNDESGRIFEVFMTPEDFLRSFTPGVM 217
Query: 202 PPSESGLVR-DGYLSGERCPGELRCPASEFFMLFDVNNDGLISFKEYIFFVTLLSIPESS 261 P GL Y + + P S F+ L +GLI+F +Y+F +TLLS + Sbjct: 218 QPRRWGLDSFKAYNPEKHKRHKFSDPNSIFYKL---GENGLINFSDYLFLMTLLSTSHAD 277
Query: 262 FSVAFKMFDLDKNGEIDRDEFKKVMALMRNQNRQGSTHRDGLRHGFKLGGSVENGGLLE- 321 F++AFK+FD+D NG +D++EF KV L+ +Q G HRD + VE LE Sbjct: 278 FALAFKIFDVDGNGALDKEEFTKVQQLIMSQTTVGQRHRDHVTPNSSF--RVETNSALET 337
Query: 322 YFFGETGEMQLHYDRFVRFLRKLHDEIVRLEFSHYD--YKSQGTISAKDFALSMVASADL 381 YFFG+ G+ L ++F+ F +L +I+++EF D S G I+ + FA ++ A + Sbjct: 338 YFFGKDGKGSLSSEKFIEFQERLQHDILKMEFERRDAMENSDGLITEESFAQLLLLHAQI 397
Query: 382 -----NHVNKLLERVDELDNCPELSSTRISEDQFCKFAELRTKLEPFSLALLAYGKVNGL 441 H+ K ++R + D S +S D+ F E ++ +AL + Sbjct: 398 AEKKQKHMLKRVKRRFKGD-----QSKGVSFDETKAFFEFLYHIDDVDIALHFHKMAGMS 457
Query: 442 LTRKDFQRAASQVCGVDLNDNVVDIVFHVFDANRDGNLCAEEFLRVLQKR-ARDIAQPNE 496 + K +R A +V G+ L+D+VVD+V +FD N DG L EE + V+++R R + +P + Sbjct: 458 IDAKLLKRVAVKVTGIPLSDHVVDVVITLFDDNLDGKLSHEEMVAVMRRRMRRGLERPRD 517
Query: 120 SSLDSVPRSLSLKNSRFLFPDAFRRKVFFKYEKRMRMRSPPEKVFEYFASV----QSSSG 179 SS + P+ K +R F D RKV +YE R+R S P+K+F YFA++ +S Sbjct: 85 SSDEDQPQEGKKKKARVGFRD---RKVM-EYENRIRAYSTPDKIFRYFATLKVIHESGES 144
Query: 180 EILMTPSDLMRAVVPVFPPSESGLVRDGYL----SGERCPGELRCPASEFFMLFDVNNDG 239 E+ MTP D +R++ P E+ L D ++ G++ E A E + + + G Sbjct: 145 EVFMTPQDFVRSITPNEKQPEN-LGLDQFIIKRYDGKKISQEREKFADEDSIFYSLGECG 204
Query: 300 GLRHGFKLGGSVENGGLLEYFFGETGEMQLHYDRFVRFLRKLHDEIVRLEFSHYDYKSQG 359 G L + L YFFG + +L F+ F RKL ++++LEF +D G Sbjct: 265 RSTTGNTLKTGFSSA-LTTYFFGADLKGKLTIKNFLEFQRKLQHDVLKLEFERHD-PVDG 324
Query: 360 TISAKDF-----ALSMVASADLNHVNKLLERVDELDNCPELSSTRISEDQFCKFAELRTK 419 I+ + F A S V S L H+ K L++ + + ++ ++ F Sbjct: 325 HITERQFGSMLLAYSGVQSKKLTHMLKQLKKRFK-------DAEGLTFEEVENFFTFLKN 384
Query: 420 LEPFSLALLAYGKVNGLLTRKDFQRAASQVCGVDLNDNVVDIVFHVFDANRDGNLCAEEF 479 + AL Y L + Q+ A V V+L+D+V D+VF +FD + +G L +EF Sbjct: 385 INDVDTALSFYHMAGASLDKVTMQQVARTVAKVELSDHVCDVVFALFDCDGNGELSNKEF 444
Query: 480 LRVLQKR-ARDIAQPNETGILGFLSCCRNCANNCSF 502 + ++++R R + +P + G + CA ++ Sbjct: 445 IAIMKQRLMRGLEKPKDMGFTRLMRAMWKCAQETAW 466
Query: 262 SFSVAFKMFDLDKNGEIDRDEFKKVMALMRNQNRQGSTHRDGLRHGFKLGGSVENGGLLE 321 +F +AFKMFDL+ +GE+D +EF++V +++R+Q G HRD G L + L Sbjct: 233 NFEIAFKMFDLNGDGEVDLEEFEQVQSIIRSQTSMGMRHRDRSTTGNTLKTGGCSSALTT 292
Query: 322 YFFGETGEMQLHYDRFVRFLRKLHDEIVRLEFSHYDYKSQGTISAKDF-----ALSMVAS 381 YFFG + +L F+ F RKL ++++LEF D G I+ K F A S V S Sbjct: 293 YFFGADLKGKLTISSFLEFQRKLQHDVLKLEFERND-PVDGRITEKQFGGMLLAYSGVQS 352
Query: 382 ADLNHVNKLLERVDELDNCPELSSTRISEDQFCKFAELRTKLEPFSLALLAYGKVNGLLT 441 L + K L+R+ + + I+ ++ F + AL Y + Sbjct: 353 RKLKQMQKNLKRMFK-------DAQGITFEEVENFFTFLKNVNDVDTALSFYHMAGASID 412
Query: 442 RKDFQRAASQVCGVDLNDNVVDIVFHVFDANRDGNLCAEEFLRVLQKR-ARDIAQPNETG 501 + ++ A V V+L+D+V D+VF +FD + +G L +EF+ ++++R R + +P + G Sbjct: 413 KATMKQVARTVAKVELSDHVCDVVFALFDCDGNGELSNKEFIAIMKQRLMRGLEKPKDMG 472
Query: 56 KLVSGIAVGSSLGALFYPVSPNHDCDVNDNVSSSSSSSVFDYFKGKSMLTFADWSSAPTE 115 K ++GI+ GS+LG L++ SS SS S+ F G ++L+FAD SS P+ Sbjct: 62 KWITGISAGSALGFLYW--------------SSGSSDSISGLFGGSNLLSFAD-SSTPS- 121
Query: 116 ATSISSLDSVPRSLSLK------NSRFLFPDAFRRKVFFKYEKRMRMRSPPEKVFEYFAS 175 + D PRS K +S F+F DA+RRK+FF YEKR+R++SPPEKVFEYFAS Sbjct: 122 VCGVKVGDLKPRSFIPKLSLPGYSSGFIFGDAYRRKIFFNYEKRLRLQSPPEKVFEYFAS 181
Query: 236 DGLISFKEYIFFVTLLSIPESSFSVAFKMFDLDKNGEIDRDEFKKVMALMRNQNRQGSTH 295 DGLISFKEYIFFVTLLSIPESSF+VAFKMFD D NGEID++EFK VM+LMR+Q+RQG H Sbjct: 242 DGLISFKEYIFFVTLLSIPESSFAVAFKMFDTDNNGEIDKEEFKTVMSLMRSQHRQGVGH 301
Query: 296 RDGLRHGFKLGGSVENGGLLEYFFGETGEMQLHYDRFVRFLRKLHDEIVRLEFSHYDYKS 355 RDGLR G + GSVE+GGL+EYFFG+ G +L +D+F +F++ L +E++RLEF+HYDYK Sbjct: 302 RDGLRTGLHMTGSVEDGGLVEYFFGKDGSQKLKHDKFTKFMKDLTEEMLRLEFAHYDYKR 361
Query: 356 QGTISAKDFALSMVASADLNHVNKLLERVDELDNCPELSSTRISEDQFCKFAELRTKLEP 415 +G+ISAKDFALSMVA+AD +H++KLL+RV+ L P L RIS +F +F ELR+KL P Sbjct: 362 RGSISAKDFALSMVAAADASHLSKLLDRVESLSEHPHLRDMRISLKEFKQFDELRSKLGP 421
