Spo09984 (gene) | SpinachBase

Overview


Name	Spo09984
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Heterotrimeric G protein gamma subunit 4
Location	chr1 : 45304440 .. 45306652 (+)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGAGCAGCCGATGCCAAAATCACCTCCCGGGTTGTTCGCCAAGCGCCGCCACCAGGCTAAACTTCAGGTTTTGGAGAGAGAAATTGGTTTTCTTGAGGTAATTTTATTCTCTCATTTTTTGTGTTTTTATTGAAACAAACGTTATTTCTCTCTTTTTAAGTATGTAACAGCTGAGGGAATATTCATTCATAGATTCAAATGTATGATTGTATGTTTGTAACATGCAAGTACTCCGTAGTATGCAACTGTGCAAGGAATTTCATTGCTTTATTTGGCTATGATTTGTGTACCCCAACTAGTTTCGTGGCCCCAAAGTCTTGGGGCCGCACCAAAGATCGAATCTTGTCTACATTCAATCATGCCTGTGTAATTTGTTACACGTACTAAGCTTGATAAAAGTGTCCACCAAGGCTGCAGGGTAGCACTAGAACGAAGAAGAACTAGCTTGAGAAATATTTAAGGCTAAATTAATAGTAGAAGTTTTTCTGATGAACTAAGAATCAAGCAATCCTTTTCCCCCCATCTTACAAGACTTATCTTGATAAATATACCTGAATTGTCATTAAAGGTATTCCATTTCTTCATTCCTATGAACCTTTAAGCCTTAGTTCTTGTCTATATAGTTGAATCGTTCCTTCGAAAACTTATCCATCCATCAAAACCAAATTTCTTTCGGAATATATACAAGCCTTTTGCATATTTTGTTTCCCTTCACAATGCCAATGAAACCGACAACCACCATGTCTTCTGAGGCAATGCAGCCGAACCCACCATCTAACGAAGGGGAGAAGCACAAACAGTTAGACCACGAGATTAGAGAGATGGTCTCTTCTCTTACCTCCCGCCTCAATGATCTTCATCAAAGCCATAAGCCGGGAGGGAGTGGGCCCCATATGGGGGACGAAGATGAAAACGGAGTTAGAATCATCACGATTGCTGGGACCAACACAGGGGCTACCATGAAGCGTGAATTGTTGGATCATCACCATAAACATCACCACCACCACCACCACCACGAGGTATTCCCCGATGATGATCAGCCTGAAGGTTTGGACACCTATGTGAACAGCAACTCCCAAGCCGTCAACAACTCGATCATGCTTGGTGCTAGCTACACCAGCAATGATCCAGGTGTCCACATGGACATTACAGATGTAGTGGACACCCATGGCCGGAAGGTCGAGAAGCACGGGAGAAGAGGAATAAGAAAGGGAAAGGAGCCTGCTCAAGCCAACTTACAGTCTGAGTTTTCTGAATGATCATCTGAGTTCTGACAAGGAATCAAACATGACCAAATAATTGCATTTCTTGTAATATTCTAGCCTCCGACGTGTAAGAGAAAATTATGTGATTAAGTCAAATCGAAAACTTTACAAATAGAATGTGTGGATGTATGTGATAGGTTGAGCCGTTGAGTAGTTCTTGATTCTGTTGTGTGTACCTGTTTTTGGAGTTGTACCTCTAAATTCACAATAATGGGAATATCTAGCTATCTTCAGTTTTAAAACTGTGTTATAATGACAGTAATGGAATCAGATTATTTCTCTACTCATGCATTTAACCTAGACACTGTTGATCTCATGAAAAACAGGAGGAGTTGAAGTCACTTGACAACATCCAGCCTACTTCTAGGTGTTGCAAAGAGTATGTATGGCTCGTTGACTGTCTGATTTAGCTAATTTTATAATCCTTCACAGATTTATCTTTTGTTCTTTTGTTTGTTTTCATCTATTGATCTCAGTGTCTTTTTCTTTGATGTAATGCTAATTAACAGGATCAATGACTTCGTCGTTACAAAACCAGATCCCTTGATAACTACGTATGATAATTCTACGCGTTATCTAAAATCTTGTGACTGTATAGTACAGAGTATATTGATATGGAAATAAACTTTTTCCCCTGATGGTTATTTGCAGAAATCAGAAGTCTAGTCCGTCAGACTGTTCGTGTTCATGTAGGAGAATCAGGTACTTTTCTTTAAAAAAATCCCTTGGCTGTTCAAATCTCGGTCTGTTTACAGAATATATGCAAGATCGTACCATAGTATTAATAATATCTGAAACATGACCAGGGGGAAATCATGTTTTAAATCGTCGTGGACTTGCTGTTTCAGTGGATGCTGCCGATCAGATAAAGAGCATCGCCAGCAAGCACGAACAAATTGCTGTTGTTCGAGGGAGAGTTATTGTCCTAAATGTTGTGCCATAA

mRNA sequence

ATGGCGGAGCAGCCGATGCCAAAATCACCTCCCGGGTTGTTCGCCAAGCGCCGCCACCAGGCTAAACTTCAGGTTTTGGAGAGAGAAATTGGTTTTCTTGAGGAGGAGTTGAAGTCACTTGACAACATCCAGCCTACTTCTAGGTGTTGCAAAGAGATCAATGACTTCGTCGTTACAAAACCAGATCCCTTGATAACTACAAATCAGAAGTCTAGTCCGTCAGACTGTTCGTGTTCATGGGGAAATCATGTTTTAAATCGTCGTGGACTTGCTGTTTCAGTGGATGCTGCCGATCAGATAAAGAGCATCGCCAGCAAGCACGAACAAATTGCTGTTGTTCGAGGGAGAGTTATTGTCCTAAATGTTGTGCCATAA

Coding sequence (CDS)

ATGGCGGAGCAGCCGATGCCAAAATCACCTCCCGGGTTGTTCGCCAAGCGCCGCCACCAGGCTAAACTTCAGGTTTTGGAGAGAGAAATTGGTTTTCTTGAGGAGGAGTTGAAGTCACTTGACAACATCCAGCCTACTTCTAGGTGTTGCAAAGAGATCAATGACTTCGTCGTTACAAAACCAGATCCCTTGATAACTACAAATCAGAAGTCTAGTCCGTCAGACTGTTCGTGTTCATGGGGAAATCATGTTTTAAATCGTCGTGGACTTGCTGTTTCAGTGGATGCTGCCGATCAGATAAAGAGCATCGCCAGCAAGCACGAACAAATTGCTGTTGTTCGAGGGAGAGTTATTGTCCTAAATGTTGTGCCATAA

Protein sequence

MAEQPMPKSPPGLFAKRRHQAKLQVLEREIGFLEEELKSLDNIQPTSRCCKEINDFVVTKPDPLITTNQKSSPSDCSCSWGNHVLNRRGLAVSVDAADQIKSIASKHEQIAVVRGRVIVLNVVP

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo09984.1	Spo09984.1	mRNA

Homology

[Close]

BLAST of Spo09984.1 vs. NCBI nr
Match: gi|731348052|ref|XP_010685299.1| (PREDICTED: guanine nucleotide-binding protein subunit gamma 3-like isoform X2 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 126.3 bits (316), Expect = 3.600e-26
Identity = 63/85 (74.12%), Postives = 70/85 (82.35%), Query Frame = 1


		  
Query: 1  MAEQPMPKSPPG---LFAKRRHQAKLQVLEREIGFLEEELKSLDNIQPTSRCCKEINDFV 60
          MAEQPMPKSPPG   L+AKRRHQAKLQVLEREI FL+EELKSL+ +QPTSRCC+EINDFV
Sbjct: 1  MAEQPMPKSPPGYIDLYAKRRHQAKLQVLEREIDFLQEELKSLEKVQPTSRCCEEINDFV 60

Query: 61 VTKPDPLITTNQK--SSPSDCSCSW 81
            KPDP +   QK  S+ S CSCSW
Sbjct: 61 AAKPDPFVAEYQKSNSNQSGCSCSW 85

[Close]

BLAST of Spo09984.1 vs. NCBI nr
Match: gi|731348050|ref|XP_010685298.1| (PREDICTED: guanine nucleotide-binding protein subunit gamma 3-like isoform X1 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 126.3 bits (316), Expect = 3.600e-26
Identity = 63/85 (74.12%), Postives = 70/85 (82.35%), Query Frame = 1


		  
Query: 1  MAEQPMPKSPPG---LFAKRRHQAKLQVLEREIGFLEEELKSLDNIQPTSRCCKEINDFV 60
          MAEQPMPKSPPG   L+AKRRHQAKLQVLEREI FL+EELKSL+ +QPTSRCC+EINDFV
Sbjct: 1  MAEQPMPKSPPGYIDLYAKRRHQAKLQVLEREIDFLQEELKSLEKVQPTSRCCEEINDFV 60

Query: 61 VTKPDPLITTNQK--SSPSDCSCSW 81
            KPDP +   QK  S+ S CSCSW
Sbjct: 61 AAKPDPFVAEYQKSNSNQSGCSCSW 85

[Close]

BLAST of Spo09984.1 vs. NCBI nr
Match: gi|694359088|ref|XP_009359725.1| (PREDICTED: guanine nucleotide-binding protein subunit gamma 3-like [Pyrus x bretschneideri])

HSP 1 Score: 97.1 bits (240), Expect = 2.400e-17
Identity = 52/90 (57.78%), Postives = 61/90 (67.78%), Query Frame = 1


		  
Query: 5   PMPKSPP---GLFAKRRHQAKLQVLEREIGFLEEELKSLDNIQPTSRCCKEINDFVVTKP 64
           P PKSPP    L+ KRR  A++Q+LEREIGFLE+ELKS++ +QP SRCCKEI DFVV  P
Sbjct: 17  PRPKSPPQYPDLYGKRRETARVQMLEREIGFLEDELKSVERLQPASRCCKEITDFVVANP 76

Query: 65  DPLITTNQKSSPS----------DC-SCSW 81
           DPLI TN+K   S           C SCSW
Sbjct: 77  DPLIPTNRKKRRSCRFWKWLCGMPCFSCSW 106

[Close]

BLAST of Spo09984.1 vs. NCBI nr
Match: gi|658005399|ref|XP_008337845.1| (PREDICTED: guanine nucleotide-binding protein subunit gamma 3 [Malus domestica])

HSP 1 Score: 96.7 bits (239), Expect = 3.100e-17
Identity = 52/90 (57.78%), Postives = 61/90 (67.78%), Query Frame = 1


		  
Query: 5   PMPKSPP---GLFAKRRHQAKLQVLEREIGFLEEELKSLDNIQPTSRCCKEINDFVVTKP 64
           P PKSPP    L+ KRR  A++Q+LEREIGFLE+ELKS++ +QP SRCCKEI DFVV  P
Sbjct: 17  PHPKSPPQYPDLYGKRRETARVQMLEREIGFLEDELKSVERLQPASRCCKEITDFVVANP 76

Query: 65  DPLITTNQKSSPS----------DC-SCSW 81
           DPLI TN+K   S           C SCSW
Sbjct: 77  DPLIPTNRKKRRSCRFWKWLCGMPCFSCSW 106

[Close]

BLAST of Spo09984.1 vs. NCBI nr
Match: gi|694418411|ref|XP_009337215.1| (PREDICTED: guanine nucleotide-binding protein subunit gamma 3-like [Pyrus x bretschneideri])

HSP 1 Score: 96.7 bits (239), Expect = 3.100e-17
Identity = 52/90 (57.78%), Postives = 61/90 (67.78%), Query Frame = 1


		  
Query: 5   PMPKSPP---GLFAKRRHQAKLQVLEREIGFLEEELKSLDNIQPTSRCCKEINDFVVTKP 64
           P PKSPP    L+ KRR  A++Q+LEREIGFLEEELKS++ +QP SRCCKE+ DFVV  P
Sbjct: 17  PRPKSPPQYPDLYGKRRETARVQMLEREIGFLEEELKSVERLQPASRCCKEVVDFVVANP 76

Query: 65  DPLITTNQKSSPS----------DC-SCSW 81
           DPLI TN+K   S           C SCSW
Sbjct: 77  DPLIPTNRKKRRSCRFWKWLCGMPCFSCSW 106

[Close]

BLAST of Spo09984.1 vs. UniProtKB/TrEMBL
Match: M0SC65_MUSAM (Uncharacterized protein OS=Musa acuminata subsp. malaccensis PE=4 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 4.300e-18
Identity = 49/85 (57.65%), Postives = 60/85 (70.59%), Query Frame = 1


		  
Query: 5   PMPKSPP---GLFAKRRHQAKLQVLEREIGFLEEELKSLDNIQPTSRCCKEINDFVVTKP 64
           P PKSPP    L  +RR Q +LQ+L RE+GF+EEEL+SL+ IQP SRCCKE+NDFV TKP
Sbjct: 21  PSPKSPPKYPDLCGRRRLQLELQILNREVGFIEEELQSLEGIQPVSRCCKEVNDFVGTKP 80

Query: 65  DPLITTNQKSSPSDCSCSWGNHVLN 87
           DPLI  N++   S C   W  + LN
Sbjct: 81  DPLIPINKRRHKSCCLWRWIRYFLN 105

[Close]

BLAST of Spo09984.1 vs. UniProtKB/TrEMBL
Match: A0A067KFZ1_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_07494 PE=4 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 3.700e-17
Identity = 48/73 (65.75%), Postives = 54/73 (73.97%), Query Frame = 1


		  
Query: 5  PMPKSPP---GLFAKRRHQAKLQVLEREIGFLEEELKSLDNIQPTSRCCKEINDFVVTKP 64
          P PKSPP    L+ KRR  AK+Q+LEREIGFLEEELKS+  + P SRCCKEI DFVV  P
Sbjct: 26 PCPKSPPEYPDLYGKRREMAKVQMLEREIGFLEEELKSVQGLHPASRCCKEITDFVVANP 85

Query: 65 DPLITTNQKSSPS 75
          DPLI TN+K   S
Sbjct: 86 DPLIPTNRKKRRS 98

[Close]

BLAST of Spo09984.1 vs. UniProtKB/TrEMBL
Match: M5VU58_PRUPE (Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa021140mg PE=4 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 6.300e-17
Identity = 47/79 (59.49%), Postives = 57/79 (72.15%), Query Frame = 1


		  
Query: 5  PMPKSPP---GLFAKRRHQAKLQVLEREIGFLEEELKSLDNIQPTSRCCKEINDFVVTKP 64
          P PKSPP    L+ KRR  A++Q+LEREIGFLEEELKS++ +QP+SRCCKE+ DFV   P
Sbjct: 13 PCPKSPPQYPDLYGKRRETARVQMLEREIGFLEEELKSVERLQPSSRCCKEVADFVAANP 72

Query: 65 DPLITTNQKSSPSDCSCSW 81
          DPLI TN+K   S     W
Sbjct: 73 DPLIPTNRKKRRSCRFWKW 91

[Close]

BLAST of Spo09984.1 vs. UniProtKB/TrEMBL
Match: U5GAQ9_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0006s14330g PE=4 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 1.800e-16
Identity = 47/79 (59.49%), Postives = 55/79 (69.62%), Query Frame = 1


		  
Query: 5  PMPKSPP---GLFAKRRHQAKLQVLEREIGFLEEELKSLDNIQPTSRCCKEINDFVVTKP 64
          P PKSPP    L+ KRR  AK+Q+LEREIGFLEEELKSL  +QP SRCCKE+ DFVV   
Sbjct: 18 PCPKSPPEYPDLYGKRREMAKVQMLEREIGFLEEELKSLQGLQPASRCCKEVTDFVVANS 77

Query: 65 DPLITTNQKSSPSDCSCSW 81
          DPLI T++K   +     W
Sbjct: 78 DPLIPTSRKKRRTCLFWKW 96

[Close]

BLAST of Spo09984.1 vs. UniProtKB/TrEMBL
Match: W9QSH3_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_027404 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 6.900e-16
Identity = 56/123 (45.53%), Postives = 69/123 (56.10%), Query Frame = 1


		  
Query: 5   PMPKSPPG---LFAKRRHQAKLQVLEREIGFLEEELKSLDNIQPTSRCCKEINDFVVTKP 64
           P PKSPP    L+ KRR  AK+Q+LEREIGFL+EELKS + IQP+SRCCKEI +FVV+ P
Sbjct: 21  PCPKSPPAYPDLYGKRREMAKVQMLEREIGFLQEELKSAERIQPSSRCCKEITEFVVSNP 80

Query: 65  DPLITTNQKSSPSDCSCSWGNHVLNRRGLAVSVDAADQIKSIASKHEQIAVVRGRVIVLN 124
           DPLI T   +                   A +  A DQI    S+  +IA     V  L 
Sbjct: 81  DPLIPTAAVALRVSLVRYQSGVAAGAAAAAAAAHAPDQIAVKTSRAAEIAAFFPPVHALI 140

[Close]

BLAST of Spo09984.1 vs. ExPASy Swiss-Prot
Match: GG3_ARATH (Guanine nucleotide-binding protein subunit gamma 3 OS=Arabidopsis thaliana GN=GG3 PE=2 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 2.900e-15
Identity = 42/79 (53.16%), Postives = 53/79 (67.09%), Query Frame = 1


		  
Query: 5   PMPKSPP---GLFAKRRHQAKLQVLEREIGFLEEELKSLDNIQPTSRCCKEINDFVVTKP 64
           P PKSPP    L+ KRR  A++Q+LEREIGFLE E+K ++ +QP SRC KE++DFVV   
Sbjct: 33  PRPKSPPEYPDLYGKRREAARVQMLEREIGFLEGEIKFIEGVQPASRCIKEVSDFVVANS 92

Query: 65  DPLITTNQKSSPSDCSCSW 81
           DPLI   +KS  S     W
Sbjct: 93  DPLIPAQRKSRRSFRFWKW 111

[Close]

BLAST of Spo09984.1 vs. TAIR (Arabidopsis)
Match: AT5G20635.1 (FUNCTIONS IN: molecular_function unknown)

HSP 1 Score: 82.8 bits (203), Expect = 1.600e-16
Identity = 42/79 (53.16%), Postives = 53/79 (67.09%), Query Frame = 1


		  
Query: 5   PMPKSPP---GLFAKRRHQAKLQVLEREIGFLEEELKSLDNIQPTSRCCKEINDFVVTKP 64
           P PKSPP    L+ KRR  A++Q+LEREIGFLE E+K ++ +QP SRC KE++DFVV   
Sbjct: 33  PRPKSPPEYPDLYGKRREAARVQMLEREIGFLEGEIKFIEGVQPASRCIKEVSDFVVANS 92

Query: 65  DPLITTNQKSSPSDCSCSW 81
           DPLI   +KS  S     W
Sbjct: 93  DPLIPAQRKSRRSFRFWKW 111

[Close]

BLAST of Spo09984.1 vs. TAIR (Arabidopsis)
Match: AT3G22942.1 (G-protein gamma subunit 2)

HSP 1 Score: 50.4 bits (119), Expect = 9.000e-7
Identity = 26/50 (52.00%), Postives = 34/50 (68.00%), Query Frame = 1


		  
Query: 16 KRRHQAKLQVLEREIGFLEEELKSLDNIQPTSRCCKEINDFVVTKPDPLI 66
          K R QA+L+ LE+E  FLEEEL+ L+ +   S  CKE  D V +KPDPL+
Sbjct: 22 KHRIQAELKRLEQEARFLEEELEQLEKMDNASASCKEFLDSVDSKPDPLL 71

The following BLAST results are available for this feature:

BLAST of Spo09984.1 vs. NCBI nr
BLAST of Spo09984.1 vs. UniProtKB/TrEMBL
BLAST of Spo09984.1 vs. ExPASy Swiss-Prot
BLAST of Spo09984.1 vs. TAIR (Arabidopsis)

BLAST of Spo09984.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|731348052\|ref\|XP_010685299.1\|	3.6e-26	74.1	PREDICTED: guanine nucleotide-...	[more]
gi\|731348050\|ref\|XP_010685298.1\|	3.6e-26	74.1	PREDICTED: guanine nucleotide-...	[more]
gi\|694359088\|ref\|XP_009359725.1\|	2.4e-17	57.7	PREDICTED: guanine nucleotide-...	[more]
gi\|658005399\|ref\|XP_008337845.1\|	3.1e-17	57.7	PREDICTED: guanine nucleotide-...	[more]
gi\|694418411\|ref\|XP_009337215.1\|	3.1e-17	57.7	PREDICTED: guanine nucleotide-...	[more]

BLAST of Spo09984.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
M0SC65_MUSAM	4.3e-18	57.6	Uncharacterized protein OS=Mus...	[more]
A0A067KFZ1_JATCU	3.7e-17	65.7	Uncharacterized protein OS=Jat...	[more]
M5VU58_PRUPE	6.3e-17	59.4	Uncharacterized protein (Fragm...	[more]
U5GAQ9_POPTR	1.8e-16	59.4	Uncharacterized protein OS=Pop...	[more]
W9QSH3_9ROSA	6.9e-16	45.5	Uncharacterized protein OS=Mor...	[more]

BLAST of Spo09984.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 1


Match Name	E-value	Identity	Description
GG3_ARATH	2.9e-15	53.1	Guanine nucleotide-binding pro...	[more]

BLAST of Spo09984.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 2


Match Name	E-value	Identity	Description
AT5G20635.1	1.6e-16	53.1	FUNCTIONS IN: molecular_functi...	[more]
AT3G22942.1	9.0e-7	52.0	G-protein gamma subunit 2	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015898	G-protein gamma-like domain	PFAM	PF00631	G-gamma	coord: 24..70 score: 2.4
None	No IPR available	unknown	Coil	Coil	coord: 16..43 scor
None	No IPR available	PANTHER	PTHR32378	FAMILY NOT NAMED	coord: 2..80 score: 1.7
None	No IPR available	PANTHER	PTHR32378:SF9	KERATIN-ASSOCIATED PROTEIN 29-1	coord: 2..80 score: 1.7
None	No IPR available	SMART	SM01224	G_gamma_2	coord: 19..81 score: 1.

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007186	G-protein coupled receptor signaling pathway
biological_process	GO:0009737	response to abscisic acid
cellular_component	GO:0005834	heterotrimeric G-protein complex
molecular_function	GO:0004871	obsolete signal transducer activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo18564	0.67	Barchart \| Table