Spo10180 (gene)

Overview
NameSpo10180
Typegene
OrganismSpinacia oleracea (Spinach)
DescriptionTropinone reductase-like protein 16
LocationSpoScf_06484 : 766 .. 981 (+)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GGACTTAAGAATTACAAGGAGTATAAGGAGGCTGTCGAGTCACGAATTGCTCTTGGACGTATTGGGAACTCGGATGAAATTTCAGCTGTAGTTGCGTTTTTATGTCTACCAGCAGCTTCTTATATAACTGGACAAACTATAACCGTCGATGGAGGTTTTACTATTAATGCCTTCATACCACTTCCGCTTAATGAGAAGGCCAAGGAAAATAATTGA

mRNA sequence

GGACTTAAGAATTACAAGGAGTATAAGGAGGCTGTCGAGTCACGAATTGCTCTTGGACGTATTGGGAACTCGGATGAAATTTCAGCTGTAGTTGCGTTTTTATGTCTACCAGCAGCTTCTTATATAACTGGACAAACTATAACCGTCGATGGAGGTTTTACTATTAATGCCTTCATACCACTTCCGCTTAATGAGAAGGCCAAGGAAAATAATTGA

Coding sequence (CDS)

GGACTTAAGAATTACAAGGAGTATAAGGAGGCTGTCGAGTCACGAATTGCTCTTGGACGTATTGGGAACTCGGATGAAATTTCAGCTGTAGTTGCGTTTTTATGTCTACCAGCAGCTTCTTATATAACTGGACAAACTATAACCGTCGATGGAGGTTTTACTATTAATGCCTTCATACCACTTCCGCTTAATGAGAAGGCCAAGGAAAATAATTGA

Protein sequence

GLKNYKEYKEAVESRIALGRIGNSDEISAVVAFLCLPAASYITGQTITVDGGFTINAFIPLPLNEKAKENN
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo10180.1Spo10180.1mRNA


Homology
[Close]
BLAST of Spo10180.1 vs. NCBI nr
Match: gi|902239283|gb|KNA25545.1| (hypothetical protein SOVF_004770 [Spinacia oleracea])

HSP 1 Score: 142.9 bits (359), Expect = 2.200e-31
Identity = 71/71 (100.00%), Postives = 71/71 (100.00%), Query Frame = 1 

		  

Query: 1   GLKNYKEYKEAVESRIALGRIGNSDEISAVVAFLCLPAASYITGQTITVDGGFTINAFIP 60
           GLKNYKEYKEAVESRIALGRIGNSDEISAVVAFLCLPAASYITGQTITVDGGFTINAFIP
Sbjct: 188 GLKNYKEYKEAVESRIALGRIGNSDEISAVVAFLCLPAASYITGQTITVDGGFTINAFIP 247

Query: 61  LPLNEKAKENN 72
           LPLNEKAKENN
Sbjct: 248 LPLNEKAKENN 258
[Close]
BLAST of Spo10180.1 vs. NCBI nr
Match: gi|379319201|gb|AFC98466.1| (short chain alcohol dehydrogenase-like protein [Atriplex canescens])

HSP 1 Score: 103.6 bits (257), Expect = 1.400e-19
Identity = 48/69 (69.57%), Postives = 61/69 (88.41%), Query Frame = 1 

		  

Query: 2   LKNYKEYKEAVESRIALGRIGNSDEISAVVAFLCLPAASYITGQTITVDGGFTINAFIPL 61
           LK++KEY +AVESRIALGR+G  +EIS VVAFLC+PA+SYITGQTITVDGGFT+N FIP+
Sbjct: 86  LKSHKEYLKAVESRIALGRVGEPEEISPVVAFLCMPASSYITGQTITVDGGFTVNGFIPI 145

Query: 62  PLNEKAKEN 71
            +N+  +E+
Sbjct: 146 LVNKTGEES 154
[Close]
BLAST of Spo10180.1 vs. NCBI nr
Match: gi|870861453|gb|KMT12725.1| (hypothetical protein BVRB_4g088210 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 99.0 bits (245), Expect = 3.600e-18
Identity = 45/64 (70.31%), Postives = 56/64 (87.50%), Query Frame = 1 

		  

Query: 1   GLKNYKEYKEAVESRIALGRIGNSDEISAVVAFLCLPAASYITGQTITVDGGFTINAFIP 60
           G++  KEY +  ESRIALGR+G  +E+S++VA+LCLPAASYITGQTITVDGGFTINAF+P
Sbjct: 165 GMRTQKEYLKGFESRIALGRLGEPEEVSSLVAYLCLPAASYITGQTITVDGGFTINAFVP 224

Query: 61  LPLN 65
           + LN
Sbjct: 225 IVLN 228
[Close]
BLAST of Spo10180.1 vs. NCBI nr
Match: gi|731329641|ref|XP_010675702.1| (PREDICTED: tropinone reductase homolog At1g07440-like isoform X2 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 94.4 bits (233), Expect = 8.800e-17
Identity = 43/62 (69.35%), Postives = 54/62 (87.10%), Query Frame = 1 

		  

Query: 2   LKNYKEYKEAVESRIALGRIGNSDEISAVVAFLCLPAASYITGQTITVDGGFTINAFIPL 61
           L+N KE  E V+SRIAL R+G  +E+S++VA+LCLPAASYITGQTI VDGGFT+NAF+PL
Sbjct: 182 LRNQKENLEVVQSRIALRRVGEPEEVSSLVAYLCLPAASYITGQTIAVDGGFTVNAFLPL 241

Query: 62  PL 64
           P+
Sbjct: 242 PI 243
[Close]
BLAST of Spo10180.1 vs. NCBI nr
Match: gi|731329639|ref|XP_010675701.1| (PREDICTED: tropinone reductase homolog At1g07440-like isoform X1 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 94.4 bits (233), Expect = 8.800e-17
Identity = 43/62 (69.35%), Postives = 54/62 (87.10%), Query Frame = 1 

		  

Query: 2   LKNYKEYKEAVESRIALGRIGNSDEISAVVAFLCLPAASYITGQTITVDGGFTINAFIPL 61
           L+N KE  E V+SRIAL R+G  +E+S++VA+LCLPAASYITGQTI VDGGFT+NAF+PL
Sbjct: 209 LRNQKENLEVVQSRIALRRVGEPEEVSSLVAYLCLPAASYITGQTIAVDGGFTVNAFLPL 268

Query: 62  PL 64
           P+
Sbjct: 269 PI 270
[Close]
BLAST of Spo10180.1 vs. UniProtKB/TrEMBL
Match: A0A0K9S1C2_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_004770 PE=4 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 1.500e-31
Identity = 71/71 (100.00%), Postives = 71/71 (100.00%), Query Frame = 1 

		  

Query: 1   GLKNYKEYKEAVESRIALGRIGNSDEISAVVAFLCLPAASYITGQTITVDGGFTINAFIP 60
           GLKNYKEYKEAVESRIALGRIGNSDEISAVVAFLCLPAASYITGQTITVDGGFTINAFIP
Sbjct: 188 GLKNYKEYKEAVESRIALGRIGNSDEISAVVAFLCLPAASYITGQTITVDGGFTINAFIP 247

Query: 61  LPLNEKAKENN 72
           LPLNEKAKENN
Sbjct: 248 LPLNEKAKENN 258
[Close]
BLAST of Spo10180.1 vs. UniProtKB/TrEMBL
Match: H9B3S1_ATRCA (Short chain alcohol dehydrogenase-like protein OS=Atriplex canescens PE=2 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 1.000e-19
Identity = 48/69 (69.57%), Postives = 61/69 (88.41%), Query Frame = 1 

		  

Query: 2   LKNYKEYKEAVESRIALGRIGNSDEISAVVAFLCLPAASYITGQTITVDGGFTINAFIPL 61
           LK++KEY +AVESRIALGR+G  +EIS VVAFLC+PA+SYITGQTITVDGGFT+N FIP+
Sbjct: 86  LKSHKEYLKAVESRIALGRVGEPEEISPVVAFLCMPASSYITGQTITVDGGFTVNGFIPI 145

Query: 62  PLNEKAKEN 71
            +N+  +E+
Sbjct: 146 LVNKTGEES 154
[Close]
BLAST of Spo10180.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CG32_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_4g088210 PE=4 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 2.500e-18
Identity = 45/64 (70.31%), Postives = 56/64 (87.50%), Query Frame = 1 

		  

Query: 1   GLKNYKEYKEAVESRIALGRIGNSDEISAVVAFLCLPAASYITGQTITVDGGFTINAFIP 60
           G++  KEY +  ESRIALGR+G  +E+S++VA+LCLPAASYITGQTITVDGGFTINAF+P
Sbjct: 165 GMRTQKEYLKGFESRIALGRLGEPEEVSSLVAYLCLPAASYITGQTITVDGGFTINAFVP 224

Query: 61  LPLN 65
           + LN
Sbjct: 225 IVLN 228
[Close]
BLAST of Spo10180.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CKX4_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_4g088200 PE=4 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 6.100e-17
Identity = 43/62 (69.35%), Postives = 54/62 (87.10%), Query Frame = 1 

		  

Query: 2   LKNYKEYKEAVESRIALGRIGNSDEISAVVAFLCLPAASYITGQTITVDGGFTINAFIPL 61
           L+N KE  E V+SRIAL R+G  +E+S++VA+LCLPAASYITGQTI VDGGFT+NAF+PL
Sbjct: 209 LRNQKENLEVVQSRIALRRVGEPEEVSSLVAYLCLPAASYITGQTIAVDGGFTVNAFLPL 268

Query: 62  PL 64
           P+
Sbjct: 269 PI 270
[Close]
BLAST of Spo10180.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QQY6_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_156640 PE=4 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 5.700e-15
Identity = 43/47 (91.49%), Postives = 44/47 (93.62%), Query Frame = 1 

		  

Query: 1  GLKNYKEYKEAVESRIALGRIGNSDEISAVVAFLCLPAASYITGQTI 48
          GLKNYKEYKEA ESRIALGR+GN DEIS VVAFLCLPAASYITGQTI
Sbjct: 52 GLKNYKEYKEAFESRIALGRVGNPDEISPVVAFLCLPAASYITGQTI 98
[Close]
BLAST of Spo10180.1 vs. ExPASy Swiss-Prot
Match: TRNHC_ARATH (Tropinone reductase homolog At2g29360 OS=Arabidopsis thaliana GN=SDR PE=1 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 1.100e-14
Identity = 37/58 (63.79%), Postives = 48/58 (82.76%), Query Frame = 1 

		  

Query: 6   KEYKEAVESRIALGRIGNSDEISAVVAFLCLPAASYITGQTITVDGGFTINAFIPLPL 64
           +E+++ VESR  +GR+G  +E+S++VAFLCLPAASYITGQTI VDGGFT+N F   PL
Sbjct: 213 EEFRKEVESRPPMGRVGEVNEVSSLVAFLCLPAASYITGQTICVDGGFTVNGFSFKPL 270
[Close]
BLAST of Spo10180.1 vs. ExPASy Swiss-Prot
Match: TRNHF_ARATH (Tropinone reductase homolog At5g06060 OS=Arabidopsis thaliana GN=At5g06060 PE=2 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 1.800e-14
Identity = 36/53 (67.92%), Postives = 45/53 (84.91%), Query Frame = 1 

		  

Query: 6   KEYKEAVESRIALGRIGNSDEISAVVAFLCLPAASYITGQTITVDGGFTINAF 59
           KE+ EAV SR  LGR+G  +E+S++VAFLCLPA+SYITGQ I+VDGGFT+N F
Sbjct: 206 KEFVEAVVSRTPLGRVGEPEEVSSLVAFLCLPASSYITGQVISVDGGFTVNGF 258
[Close]
BLAST of Spo10180.1 vs. ExPASy Swiss-Prot
Match: TRNH4_ARATH (Tropinone reductase homolog At2g29170 OS=Arabidopsis thaliana GN=At2g29170 PE=3 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 2.400e-14
Identity = 35/55 (63.64%), Postives = 46/55 (83.64%), Query Frame = 1 

		  

Query: 4   NYKEYKEAVESRIALGRIGNSDEISAVVAFLCLPAASYITGQTITVDGGFTINAF 59
           N +E K+ VE +  +GR+GN++E+S++VAFLC PAASYITGQTI VDGGFT+N F
Sbjct: 208 NDEELKKEVERKTPMGRVGNANEVSSLVAFLCFPAASYITGQTICVDGGFTVNCF 262
[Close]
BLAST of Spo10180.1 vs. ExPASy Swiss-Prot
Match: TRNHD_ARATH (Tropinone reductase homolog At2g29370 OS=Arabidopsis thaliana GN=At2g29370 PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 9.100e-14
Identity = 37/53 (69.81%), Postives = 44/53 (83.02%), Query Frame = 1 

		  

Query: 6   KEYKEAVESRIALGRIGNSDEISAVVAFLCLPAASYITGQTITVDGGFTINAF 59
           K+ KE VES   L R+G ++E+S++VAFLCLPAASYITGQTI VDGGFTIN F
Sbjct: 213 KDVKEKVESVTPLRRVGEANEVSSLVAFLCLPAASYITGQTICVDGGFTINGF 265
[Close]
BLAST of Spo10180.1 vs. ExPASy Swiss-Prot
Match: TRNH8_ARATH (Tropinone reductase homolog At2g29310 OS=Arabidopsis thaliana GN=At2g29310 PE=2 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 2.600e-13
Identity = 34/51 (66.67%), Postives = 43/51 (84.31%), Query Frame = 1 

		  

Query: 8   YKEAVESRIALGRIGNSDEISAVVAFLCLPAASYITGQTITVDGGFTINAF 59
           +KEA+ SR  LGR G  +E++++V FLCLPAASYITGQTI +DGGFT+NAF
Sbjct: 206 FKEALFSRTPLGRAGEPNEVASLVVFLCLPAASYITGQTICIDGGFTVNAF 256
[Close]
BLAST of Spo10180.1 vs. TAIR (Arabidopsis)
Match: AT2G29360.1 (NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 80.1 bits (196), Expect = 6.100e-16
Identity = 37/58 (63.79%), Postives = 48/58 (82.76%), Query Frame = 1 

		  

Query: 6   KEYKEAVESRIALGRIGNSDEISAVVAFLCLPAASYITGQTITVDGGFTINAFIPLPL 64
           +E+++ VESR  +GR+G  +E+S++VAFLCLPAASYITGQTI VDGGFT+N F   PL
Sbjct: 213 EEFRKEVESRPPMGRVGEVNEVSSLVAFLCLPAASYITGQTICVDGGFTVNGFSFKPL 270
[Close]
BLAST of Spo10180.1 vs. TAIR (Arabidopsis)
Match: AT5G06060.1 (NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 79.3 bits (194), Expect = 1.000e-15
Identity = 36/53 (67.92%), Postives = 45/53 (84.91%), Query Frame = 1 

		  

Query: 6   KEYKEAVESRIALGRIGNSDEISAVVAFLCLPAASYITGQTITVDGGFTINAF 59
           KE+ EAV SR  LGR+G  +E+S++VAFLCLPA+SYITGQ I+VDGGFT+N F
Sbjct: 206 KEFVEAVVSRTPLGRVGEPEEVSSLVAFLCLPASSYITGQVISVDGGFTVNGF 258
[Close]
BLAST of Spo10180.1 vs. TAIR (Arabidopsis)
Match: AT2G29370.1 (NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 77.0 bits (188), Expect = 5.100e-15
Identity = 37/53 (69.81%), Postives = 44/53 (83.02%), Query Frame = 1 

		  

Query: 6   KEYKEAVESRIALGRIGNSDEISAVVAFLCLPAASYITGQTITVDGGFTINAF 59
           K+ KE VES   L R+G ++E+S++VAFLCLPAASYITGQTI VDGGFTIN F
Sbjct: 213 KDVKEKVESVTPLRRVGEANEVSSLVAFLCLPAASYITGQTICVDGGFTINGF 265
[Close]
BLAST of Spo10180.1 vs. TAIR (Arabidopsis)
Match: AT2G29310.1 (NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 75.5 bits (184), Expect = 1.500e-14
Identity = 34/51 (66.67%), Postives = 43/51 (84.31%), Query Frame = 1 

		  

Query: 8   YKEAVESRIALGRIGNSDEISAVVAFLCLPAASYITGQTITVDGGFTINAF 59
           +KEA+ SR  LGR G  +E++++V FLCLPAASYITGQTI +DGGFT+NAF
Sbjct: 206 FKEALFSRTPLGRAGEPNEVASLVVFLCLPAASYITGQTICIDGGFTVNAF 256
[Close]
BLAST of Spo10180.1 vs. TAIR (Arabidopsis)
Match: AT1G07440.1 (NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 73.9 bits (180), Expect = 4.300e-14
Identity = 34/52 (65.38%), Postives = 42/52 (80.77%), Query Frame = 1 

		  

Query: 7   EYKEAVESRIALGRIGNSDEISAVVAFLCLPAASYITGQTITVDGGFTINAF 59
           E+K+ V SR  LGR G  +E+S++VAFLC+PAASYITGQTI VDGG T+N F
Sbjct: 209 EFKKVVISRKPLGRFGEPEEVSSLVAFLCMPAASYITGQTICVDGGLTVNGF 260
The following BLAST results are available for this feature:
BLAST of Spo10180.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902239283|gb|KNA25545.1|2.2e-31100.hypothetical protein SOVF_0047... [more]
gi|379319201|gb|AFC98466.1|1.4e-1969.5short chain alcohol dehydrogen... [more]
gi|870861453|gb|KMT12725.1|3.6e-1870.3hypothetical protein BVRB_4g08... [more]
gi|731329641|ref|XP_010675702.1|8.8e-1769.3PREDICTED: tropinone reductase... [more]
gi|731329639|ref|XP_010675701.1|8.8e-1769.3PREDICTED: tropinone reductase... [more]
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BLAST of Spo10180.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9S1C2_SPIOL1.5e-31100.Uncharacterized protein OS=Spi... [more]
H9B3S1_ATRCA1.0e-1969.5Short chain alcohol dehydrogen... [more]
A0A0J8CG32_BETVU2.5e-1870.3Uncharacterized protein OS=Bet... [more]
A0A0J8CKX4_BETVU6.1e-1769.3Uncharacterized protein OS=Bet... [more]
A0A0K9QQY6_SPIOL5.7e-1591.4Uncharacterized protein OS=Spi... [more]
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BLAST of Spo10180.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5
Match NameE-valueIdentityDescription
TRNHC_ARATH1.1e-1463.7Tropinone reductase homolog At... [more]
TRNHF_ARATH1.8e-1467.9Tropinone reductase homolog At... [more]
TRNH4_ARATH2.4e-1463.6Tropinone reductase homolog At... [more]
TRNHD_ARATH9.1e-1469.8Tropinone reductase homolog At... [more]
TRNH8_ARATH2.6e-1366.6Tropinone reductase homolog At... [more]
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BLAST of Spo10180.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5
Match NameE-valueIdentityDescription
AT2G29360.16.1e-1663.7NAD(P)-binding Rossmann-fold s... [more]
AT5G06060.11.0e-1567.9NAD(P)-binding Rossmann-fold s... [more]
AT2G29370.15.1e-1569.8NAD(P)-binding Rossmann-fold s... [more]
AT2G29310.11.5e-1466.6NAD(P)-binding Rossmann-fold s... [more]
AT1G07440.14.3e-1465.3NAD(P)-binding Rossmann-fold s... [more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016040NAD(P)-binding domainGENE3D3.40.50.720coord: 4..57
score: 1.3
IPR016040NAD(P)-binding domainunknownSSF51735NAD(P)-binding Rossmann-fold domainscoord: 5..55
score: 4.01
NoneNo IPR availablePFAMPF13561adh_short_C2coord: 2..54
score: 7.6

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016556 mRNA modification
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0015979 photosynthesis
biological_process GO:0009658 chloroplast organization
biological_process GO:0010264 myo-inositol hexakisphosphate biosynthetic process
biological_process GO:0045893 positive regulation of transcription, DNA-templated
biological_process GO:0009058 biosynthetic process
cellular_component GO:0005634 nucleus
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0003677 DNA binding
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0016765 transferase activity, transferring alkyl or aryl (other than methyl) groups
RNA-Seq Expression