Spo10770 (gene) | SpinachBase

Overview


Name	Spo10770
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Kunitz trypsin inhibitor
Location	chr1 : 41370963 .. 41371592 (-)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTCACTTTATCCTCTCAACTAGCATCATCTTCCTCTTCTTCTCCCTATCTCTTCACACCACAACCGCTGCCAACCCGGCGGTGCTCGACATTGACGGCCACCCTCTTCTTTCCAGCTCCAACTACTACATTCTCCCGGTAGTCCGCGGCCGAGGCGGGGGACTTACAGTATCACCAAGAAACGCCACTGAACTATGTCCACTCAACGCGGCCCAAGATAGCCGTGAAGTCGAAGATGGCATACCTCTTGAGTTGTACCCGGTCAACCCTAATGACAGAACAGTTTATGTCTCTACTGATCTTAACTTAGTCTTTGATGCCGTCCCTCTCTGCGTGCCATCTGGCCCGTCCTTTATTTGGCAGTTAACCTTCGACGAAGCCACCGGAAGGCGGTACGTGGGGTTCGGAGGACAAACCGGGAATCCAGGCATCGAAACGGTTAGTAATTGGTTTAAGATACAGAAGGCCGGAAGTGGAAGGTTTGATTATAAGATTGTGTTTTGCCCTAGTGTTTGCAACACTTGTAAGGTGATGTGTGGAGATGTTGGTGTTTTTGTTGAGGGTGATGGGAGGAGATTGTTGGGTATTAGAGAGCAACCACTTTTGGTTAGGTTTAAGAGAGCTTAA

mRNA sequence

ATGACTCACTTTATCCTCTCAACTAGCATCATCTTCCTCTTCTTCTCCCTATCTCTTCACACCACAACCGCTGCCAACCCGGCGGTGCTCGACATTGACGGCCACCCTCTTCTTTCCAGCTCCAACTACTACATTCTCCCGGTAGTCCGCGGCCGAGGCGGGGGACTTACAGTATCACCAAGAAACGCCACTGAACTATGTCCACTCAACGCGGCCCAAGATAGCCGTGAAGTCGAAGATGGCATACCTCTTGAGTTGTACCCGGTCAACCCTAATGACAGAACAGTTTATGTCTCTACTGATCTTAACTTAGTCTTTGATGCCGTCCCTCTCTGCGTGCCATCTGGCCCGTCCTTTATTTGGCAGTTAACCTTCGACGAAGCCACCGGAAGGCGGTACGTGGGGTTCGGAGGACAAACCGGGAATCCAGGCATCGAAACGGTTAGTAATTGGTTTAAGATACAGAAGGCCGGAAGTGGAAGGTTTGATTATAAGATTGTGTTTTGCCCTAGTGTTTGCAACACTTGTAAGGTGATGTGTGGAGATGTTGGTGTTTTTGTTGAGGGTGATGGGAGGAGATTGTTGGGTATTAGAGAGCAACCACTTTTGGTTAGGTTTAAGAGAGCTTAA

Coding sequence (CDS)

ATGACTCACTTTATCCTCTCAACTAGCATCATCTTCCTCTTCTTCTCCCTATCTCTTCACACCACAACCGCTGCCAACCCGGCGGTGCTCGACATTGACGGCCACCCTCTTCTTTCCAGCTCCAACTACTACATTCTCCCGGTAGTCCGCGGCCGAGGCGGGGGACTTACAGTATCACCAAGAAACGCCACTGAACTATGTCCACTCAACGCGGCCCAAGATAGCCGTGAAGTCGAAGATGGCATACCTCTTGAGTTGTACCCGGTCAACCCTAATGACAGAACAGTTTATGTCTCTACTGATCTTAACTTAGTCTTTGATGCCGTCCCTCTCTGCGTGCCATCTGGCCCGTCCTTTATTTGGCAGTTAACCTTCGACGAAGCCACCGGAAGGCGGTACGTGGGGTTCGGAGGACAAACCGGGAATCCAGGCATCGAAACGGTTAGTAATTGGTTTAAGATACAGAAGGCCGGAAGTGGAAGGTTTGATTATAAGATTGTGTTTTGCCCTAGTGTTTGCAACACTTGTAAGGTGATGTGTGGAGATGTTGGTGTTTTTGTTGAGGGTGATGGGAGGAGATTGTTGGGTATTAGAGAGCAACCACTTTTGGTTAGGTTTAAGAGAGCTTAA

Protein sequence

MTHFILSTSIIFLFFSLSLHTTTAANPAVLDIDGHPLLSSSNYYILPVVRGRGGGLTVSPRNATELCPLNAAQDSREVEDGIPLELYPVNPNDRTVYVSTDLNLVFDAVPLCVPSGPSFIWQLTFDEATGRRYVGFGGQTGNPGIETVSNWFKIQKAGSGRFDYKIVFCPSVCNTCKVMCGDVGVFVEGDGRRLLGIREQPLLVRFKRA

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo10770.1	Spo10770.1	mRNA

Homology

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BLAST of Spo10770.1 vs. NCBI nr
Match: gi|902082350|gb|KNA03166.1| (hypothetical protein SOVF_211800 [Spinacia oleracea])

HSP 1 Score: 431.8 bits (1109), Expect = 6.800e-118
Identity = 209/209 (100.00%), Postives = 209/209 (100.00%), Query Frame = 1


		  
Query: 1   MTHFILSTSIIFLFFSLSLHTTTAANPAVLDIDGHPLLSSSNYYILPVVRGRGGGLTVSP 60
           MTHFILSTSIIFLFFSLSLHTTTAANPAVLDIDGHPLLSSSNYYILPVVRGRGGGLTVSP
Sbjct: 1   MTHFILSTSIIFLFFSLSLHTTTAANPAVLDIDGHPLLSSSNYYILPVVRGRGGGLTVSP 60

Query: 61  RNATELCPLNAAQDSREVEDGIPLELYPVNPNDRTVYVSTDLNLVFDAVPLCVPSGPSFI 120
           RNATELCPLNAAQDSREVEDGIPLELYPVNPNDRTVYVSTDLNLVFDAVPLCVPSGPSFI
Sbjct: 61  RNATELCPLNAAQDSREVEDGIPLELYPVNPNDRTVYVSTDLNLVFDAVPLCVPSGPSFI 120

Query: 121 WQLTFDEATGRRYVGFGGQTGNPGIETVSNWFKIQKAGSGRFDYKIVFCPSVCNTCKVMC 180
           WQLTFDEATGRRYVGFGGQTGNPGIETVSNWFKIQKAGSGRFDYKIVFCPSVCNTCKVMC
Sbjct: 121 WQLTFDEATGRRYVGFGGQTGNPGIETVSNWFKIQKAGSGRFDYKIVFCPSVCNTCKVMC 180

Query: 181 GDVGVFVEGDGRRLLGIREQPLLVRFKRA 210
           GDVGVFVEGDGRRLLGIREQPLLVRFKRA
Sbjct: 181 GDVGVFVEGDGRRLLGIREQPLLVRFKRA 209

[Close]

BLAST of Spo10770.1 vs. NCBI nr
Match: gi|902194163|gb|KNA12470.1| (hypothetical protein SOVF_125760 [Spinacia oleracea])

HSP 1 Score: 297.7 bits (761), Expect = 1.500e-77
Identity = 139/209 (66.51%), Postives = 165/209 (78.95%), Query Frame = 1


		  
Query: 1   MTHFILSTSIIFLFFSLSLHTTTAANPAVLDIDGHPLLSSSNYYILPVVRGRGGGLTVSP 60
           M H+ LS + I LF SLS  TTTAANPAVLDI+GHPL   SNYYILPVVRGRGGGLT+  
Sbjct: 1   MIHYFLSAATILLFLSLSPPTTTAANPAVLDINGHPLRPRSNYYILPVVRGRGGGLTMLS 60

Query: 61  RNATELCPLNAAQDSREVEDGIPLELYPVNPNDRTVYVSTDLNLVFDAVPLCVPSGPSFI 120
           +N TE+CPL  AQ++ EV +G+PL+ Y VNP D  ++VSTDLN VFDA  +CV S     
Sbjct: 61  KNTTEVCPLYVAQENHEVSNGLPLKFYSVNPKDTKIFVSTDLNFVFDASTICVQSN---T 120

Query: 121 WQLTFDEATGRRYVGFGGQTGNPGIETVSNWFKIQKAGSGRFDYKIVFCPSVCNTCKVMC 180
           W+LT DE TGRRY+G GG+ GNPG++TVSNWFKI+KAGSG++DYKIV+C  VCN CK+MC
Sbjct: 121 WELTIDEETGRRYIGVGGEIGNPGVQTVSNWFKIEKAGSGKYDYKIVYCAGVCNFCKIMC 180

Query: 181 GDVGVFVEGDGRRLLGIREQPLLVRFKRA 210
           GD+G FVE DGRRLLG   QPLLVRFK+A
Sbjct: 181 GDIGAFVEEDGRRLLGFSGQPLLVRFKKA 206

[Close]

BLAST of Spo10770.1 vs. NCBI nr
Match: gi|731344031|ref|XP_010683193.1| (PREDICTED: miraculin-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 285.4 bits (729), Expect = 7.900e-74
Identity = 135/209 (64.59%), Postives = 162/209 (77.51%), Query Frame = 1


		  
Query: 1   MTHFILSTSIIFLFFSLSLHTTTAANPAVLDIDGHPLLSSSNYYILPVVRGRGGGLTVSP 60
           MTH I+S + IFLF  LS  T+TA N AVLDI+G PL +  NYYILPV+RGRGGGLT++ 
Sbjct: 1   MTHVIISAATIFLFLYLSPLTSTADNTAVLDINGRPLQAGYNYYILPVIRGRGGGLTMAS 60

Query: 61  RNATELCPLNAAQDSREVEDGIPLELYPVNPNDRTVYVSTDLNLVFDAVPLCVPSGPSFI 120
           +NATELCPL  AQ+  EV +G+PL+ YPVNP D+ + +S+DLN VFDA   CV S     
Sbjct: 61  KNATELCPLYVAQEDHEVSNGLPLKFYPVNPKDKRISLSSDLNFVFDAATTCVQSTG--- 120

Query: 121 WQLTFDEATGRRYVGFGGQTGNPGIETVSNWFKIQKAGSGRFDYKIVFCPSVCNTCKVMC 180
           W LT +  TGRRYVG GG+ GNPG+ETV NWFKI+K GSG++DYKIV+CP VCN CKVMC
Sbjct: 121 WSLTIEMETGRRYVGTGGEIGNPGVETVDNWFKIEKDGSGKYDYKIVYCPGVCNFCKVMC 180

Query: 181 GDVGVFVEGDGRRLLGIREQPLLVRFKRA 210
           GDVGVF+E DGRRLLG  +QPLLV FK+A
Sbjct: 181 GDVGVFIEKDGRRLLGFSDQPLLVMFKKA 206

[Close]

BLAST of Spo10770.1 vs. NCBI nr
Match: gi|731344033|ref|XP_010683194.1| (PREDICTED: miraculin-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 284.3 bits (726), Expect = 1.800e-73
Identity = 139/211 (65.88%), Postives = 167/211 (79.15%), Query Frame = 1


		  
Query: 1   MTHF-ILSTSIIFLFFSLSLHTTTAANPAVLDIDGHPLLSSSNYYILPVVRGRGGGLTVS 60
           M+H  ILS + IFLF  LS  T+TAAN AVLDI+G PL + SNYYILPV+RGRGGGL ++
Sbjct: 1   MSHVVILSAATIFLFLCLSPLTSTAANIAVLDINGRPLQARSNYYILPVIRGRGGGLRMT 60

Query: 61  PRNATELCPLN-AAQDSREVEDGIPLELYPVNPNDRTVYVSTDLNLVFDAVPLCVPSGPS 120
           P+NAT+LCPL   AQ+  E+ +G+PL+ YPVNP D+T+ +STDLN VFDA  +CV S   
Sbjct: 61  PKNATQLCPLAYVAQEGSELANGLPLKFYPVNPKDKTISLSTDLNFVFDAATICVQSTQ- 120

Query: 121 FIWQLTFDEATGRRYVGFGGQTGNPGIETVSNWFKIQKAGSGRFDYKIVFCPSVCNTCKV 180
             W+L FDE TGRRYVGFGG+ GNPG  TVSNWFKI+KA +G++DYKIVFCP VCN CKV
Sbjct: 121 --WRLAFDEVTGRRYVGFGGEIGNPGGNTVSNWFKIEKAETGKYDYKIVFCPGVCNFCKV 180

Query: 181 MCGDVGVFVEGDGRRLLGIREQPLLVRFKRA 210
            CG++GVFVE DGRRLLGI  QPLLV FK+A
Sbjct: 181 ACGEIGVFVEKDGRRLLGINNQPLLVMFKKA 208

[Close]

BLAST of Spo10770.1 vs. NCBI nr
Match: gi|902170260|gb|KNA07819.1| (hypothetical protein SOVF_168350 [Spinacia oleracea])

HSP 1 Score: 250.8 bits (639), Expect = 2.200e-63
Identity = 122/212 (57.55%), Postives = 158/212 (74.53%), Query Frame = 1


		  
Query: 1   MTHFILSTSIIFLFFSLSL--HTTTAAN-PAVLDIDGHPLLSSSNYYILPVVRGRGGGLT 60
           MT+FILS +  FLFF LS    TT AA    V DI+G PL + S YYILPV+RGRGGGLT
Sbjct: 1   MTNFILSAAT-FLFFLLSSPPSTTAAAKITPVFDINGRPLQTGSTYYILPVIRGRGGGLT 60

Query: 61  VSPRNATELCPLNAAQDSREVEDGIPLELYPVNPNDRTVYVSTDLNLVFDAVPLCVPSGP 120
           ++P N T+ CPL  AQ++ EV +G+PL++YPVNPN++ V +  D+N+VFDA  +CV S  
Sbjct: 61  MTPINTTQSCPLYVAQENHEVSNGLPLKIYPVNPNEKRVPLGGDVNIVFDAASICVQSTG 120

Query: 121 SFIWQLTFDEATGRRYVGFGGQTGNPGIETVSNWFKIQKAGSGRFDYKIVFCPSVCNTCK 180
              W L FDEATG+++V  GG  GNPG++T+SNWF+I+KAGSG +DYK+ FCP VC  C 
Sbjct: 121 ---WMLAFDEATGKQHVATGGTIGNPGVDTLSNWFRIEKAGSGMYDYKVSFCPGVCIFCT 180

Query: 181 VMCGDVGVFVEGDGRRLLGIREQPLLVRFKRA 210
           VMCGDVGVF+  DG R LG+ ++PLLV+FK+A
Sbjct: 181 VMCGDVGVFIGEDGTRFLGLTDRPLLVKFKKA 208

[Close]

BLAST of Spo10770.1 vs. UniProtKB/TrEMBL
Match: A0A0K9Q9I8_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_211800 PE=4 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 4.800e-118
Identity = 209/209 (100.00%), Postives = 209/209 (100.00%), Query Frame = 1


		  
Query: 1   MTHFILSTSIIFLFFSLSLHTTTAANPAVLDIDGHPLLSSSNYYILPVVRGRGGGLTVSP 60
           MTHFILSTSIIFLFFSLSLHTTTAANPAVLDIDGHPLLSSSNYYILPVVRGRGGGLTVSP
Sbjct: 1   MTHFILSTSIIFLFFSLSLHTTTAANPAVLDIDGHPLLSSSNYYILPVVRGRGGGLTVSP 60

Query: 61  RNATELCPLNAAQDSREVEDGIPLELYPVNPNDRTVYVSTDLNLVFDAVPLCVPSGPSFI 120
           RNATELCPLNAAQDSREVEDGIPLELYPVNPNDRTVYVSTDLNLVFDAVPLCVPSGPSFI
Sbjct: 61  RNATELCPLNAAQDSREVEDGIPLELYPVNPNDRTVYVSTDLNLVFDAVPLCVPSGPSFI 120

Query: 121 WQLTFDEATGRRYVGFGGQTGNPGIETVSNWFKIQKAGSGRFDYKIVFCPSVCNTCKVMC 180
           WQLTFDEATGRRYVGFGGQTGNPGIETVSNWFKIQKAGSGRFDYKIVFCPSVCNTCKVMC
Sbjct: 121 WQLTFDEATGRRYVGFGGQTGNPGIETVSNWFKIQKAGSGRFDYKIVFCPSVCNTCKVMC 180

Query: 181 GDVGVFVEGDGRRLLGIREQPLLVRFKRA 210
           GDVGVFVEGDGRRLLGIREQPLLVRFKRA
Sbjct: 181 GDVGVFVEGDGRRLLGIREQPLLVRFKRA 209

[Close]

BLAST of Spo10770.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QYY3_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_125760 PE=4 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 1.100e-77
Identity = 139/209 (66.51%), Postives = 165/209 (78.95%), Query Frame = 1


		  
Query: 1   MTHFILSTSIIFLFFSLSLHTTTAANPAVLDIDGHPLLSSSNYYILPVVRGRGGGLTVSP 60
           M H+ LS + I LF SLS  TTTAANPAVLDI+GHPL   SNYYILPVVRGRGGGLT+  
Sbjct: 1   MIHYFLSAATILLFLSLSPPTTTAANPAVLDINGHPLRPRSNYYILPVVRGRGGGLTMLS 60

Query: 61  RNATELCPLNAAQDSREVEDGIPLELYPVNPNDRTVYVSTDLNLVFDAVPLCVPSGPSFI 120
           +N TE+CPL  AQ++ EV +G+PL+ Y VNP D  ++VSTDLN VFDA  +CV S     
Sbjct: 61  KNTTEVCPLYVAQENHEVSNGLPLKFYSVNPKDTKIFVSTDLNFVFDASTICVQSN---T 120

Query: 121 WQLTFDEATGRRYVGFGGQTGNPGIETVSNWFKIQKAGSGRFDYKIVFCPSVCNTCKVMC 180
           W+LT DE TGRRY+G GG+ GNPG++TVSNWFKI+KAGSG++DYKIV+C  VCN CK+MC
Sbjct: 121 WELTIDEETGRRYIGVGGEIGNPGVQTVSNWFKIEKAGSGKYDYKIVYCAGVCNFCKIMC 180

Query: 181 GDVGVFVEGDGRRLLGIREQPLLVRFKRA 210
           GD+G FVE DGRRLLG   QPLLVRFK+A
Sbjct: 181 GDIGAFVEEDGRRLLGFSGQPLLVRFKKA 206

[Close]

BLAST of Spo10770.1 vs. UniProtKB/TrEMBL
Match: A0A0J8C0T9_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_6g153990 PE=4 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 5.500e-74
Identity = 135/209 (64.59%), Postives = 162/209 (77.51%), Query Frame = 1


		  
Query: 1   MTHFILSTSIIFLFFSLSLHTTTAANPAVLDIDGHPLLSSSNYYILPVVRGRGGGLTVSP 60
           MTH I+S + IFLF  LS  T+TA N AVLDI+G PL +  NYYILPV+RGRGGGLT++ 
Sbjct: 1   MTHVIISAATIFLFLYLSPLTSTADNTAVLDINGRPLQAGYNYYILPVIRGRGGGLTMAS 60

Query: 61  RNATELCPLNAAQDSREVEDGIPLELYPVNPNDRTVYVSTDLNLVFDAVPLCVPSGPSFI 120
           +NATELCPL  AQ+  EV +G+PL+ YPVNP D+ + +S+DLN VFDA   CV S     
Sbjct: 61  KNATELCPLYVAQEDHEVSNGLPLKFYPVNPKDKRISLSSDLNFVFDAATTCVQSTG--- 120

Query: 121 WQLTFDEATGRRYVGFGGQTGNPGIETVSNWFKIQKAGSGRFDYKIVFCPSVCNTCKVMC 180
           W LT +  TGRRYVG GG+ GNPG+ETV NWFKI+K GSG++DYKIV+CP VCN CKVMC
Sbjct: 121 WSLTIEMETGRRYVGTGGEIGNPGVETVDNWFKIEKDGSGKYDYKIVYCPGVCNFCKVMC 180

Query: 181 GDVGVFVEGDGRRLLGIREQPLLVRFKRA 210
           GDVGVF+E DGRRLLG  +QPLLV FK+A
Sbjct: 181 GDVGVFIEKDGRRLLGFSDQPLLVMFKKA 206

[Close]

BLAST of Spo10770.1 vs. UniProtKB/TrEMBL
Match: A0A0J8C4X4_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_6g154000 PE=4 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 1.200e-73
Identity = 139/211 (65.88%), Postives = 167/211 (79.15%), Query Frame = 1


		  
Query: 1   MTHF-ILSTSIIFLFFSLSLHTTTAANPAVLDIDGHPLLSSSNYYILPVVRGRGGGLTVS 60
           M+H  ILS + IFLF  LS  T+TAAN AVLDI+G PL + SNYYILPV+RGRGGGL ++
Sbjct: 1   MSHVVILSAATIFLFLCLSPLTSTAANIAVLDINGRPLQARSNYYILPVIRGRGGGLRMT 60

Query: 61  PRNATELCPLN-AAQDSREVEDGIPLELYPVNPNDRTVYVSTDLNLVFDAVPLCVPSGPS 120
           P+NAT+LCPL   AQ+  E+ +G+PL+ YPVNP D+T+ +STDLN VFDA  +CV S   
Sbjct: 61  PKNATQLCPLAYVAQEGSELANGLPLKFYPVNPKDKTISLSTDLNFVFDAATICVQSTQ- 120

Query: 121 FIWQLTFDEATGRRYVGFGGQTGNPGIETVSNWFKIQKAGSGRFDYKIVFCPSVCNTCKV 180
             W+L FDE TGRRYVGFGG+ GNPG  TVSNWFKI+KA +G++DYKIVFCP VCN CKV
Sbjct: 121 --WRLAFDEVTGRRYVGFGGEIGNPGGNTVSNWFKIEKAETGKYDYKIVFCPGVCNFCKV 180

Query: 181 MCGDVGVFVEGDGRRLLGIREQPLLVRFKRA 210
            CG++GVFVE DGRRLLGI  QPLLV FK+A
Sbjct: 181 ACGEIGVFVEKDGRRLLGINNQPLLVMFKKA 208

[Close]

BLAST of Spo10770.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QKP3_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_168350 PE=4 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 1.500e-63
Identity = 122/212 (57.55%), Postives = 158/212 (74.53%), Query Frame = 1


		  
Query: 1   MTHFILSTSIIFLFFSLSL--HTTTAAN-PAVLDIDGHPLLSSSNYYILPVVRGRGGGLT 60
           MT+FILS +  FLFF LS    TT AA    V DI+G PL + S YYILPV+RGRGGGLT
Sbjct: 1   MTNFILSAAT-FLFFLLSSPPSTTAAAKITPVFDINGRPLQTGSTYYILPVIRGRGGGLT 60

Query: 61  VSPRNATELCPLNAAQDSREVEDGIPLELYPVNPNDRTVYVSTDLNLVFDAVPLCVPSGP 120
           ++P N T+ CPL  AQ++ EV +G+PL++YPVNPN++ V +  D+N+VFDA  +CV S  
Sbjct: 61  MTPINTTQSCPLYVAQENHEVSNGLPLKIYPVNPNEKRVPLGGDVNIVFDAASICVQSTG 120

Query: 121 SFIWQLTFDEATGRRYVGFGGQTGNPGIETVSNWFKIQKAGSGRFDYKIVFCPSVCNTCK 180
              W L FDEATG+++V  GG  GNPG++T+SNWF+I+KAGSG +DYK+ FCP VC  C 
Sbjct: 121 ---WMLAFDEATGKQHVATGGTIGNPGVDTLSNWFRIEKAGSGMYDYKVSFCPGVCIFCT 180

Query: 181 VMCGDVGVFVEGDGRRLLGIREQPLLVRFKRA 210
           VMCGDVGVF+  DG R LG+ ++PLLV+FK+A
Sbjct: 181 VMCGDVGVFIGEDGTRFLGLTDRPLLVKFKKA 208

[Close]

BLAST of Spo10770.1 vs. ExPASy Swiss-Prot
Match: MIRA_SYNDU (Miraculin OS=Synsepalum dulcificum PE=1 SV=3)

HSP 1 Score: 177.6 bits (449), Expect = 1.500e-43
Identity = 97/215 (45.12%), Postives = 131/215 (60.93%), Query Frame = 1


		  
Query: 8   TSIIFLFFSLSLHTTTAANPA----------VLDIDGHPLLSSSNYYILPVVRGRGGGLT 67
           T +   FF +S     AANP           VLDIDG  L + +NYYI+PV+R  GGGLT
Sbjct: 5   TMLSLSFFFVSALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGGGLT 64

Query: 68  VSPR--NATELCPLNAAQDSREVEDGIPLELYPVNPNDRTVYVSTDLNLVFDAVPLCVPS 127
           VS    N T +CP    Q  +EV+   PL  +P NP +  V VSTDLN+ F A   C  +
Sbjct: 65  VSATTPNGTFVCPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPCRWT 124

Query: 128 GPSFIWQLT-FDEATGRRYVGFGGQTGNPGIETVSNWFKIQK-AGSGRFDYKIVFCPSVC 187
             S +W+L  +DE+TG+ +V  GG  GNPG ET+S+WFKI++  GSG   YK+VFCP+VC
Sbjct: 125 S-STVWRLDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGF--YKLVFCPTVC 184

Query: 188 NTCKVMCGDVGVFVEGDGRRLLGIREQPLLVRFKR 209
            +CKV CGDVG++++  GRR L + ++P    F +
Sbjct: 185 GSCKVKCGDVGIYIDQKGRRRLALSDKPFAFEFNK 216

[Close]

BLAST of Spo10770.1 vs. ExPASy Swiss-Prot
Match: ASP_THECC (21 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 5.600e-27
Identity = 71/211 (33.65%), Postives = 109/211 (51.66%), Query Frame = 1


		  
Query: 7   STSIIFLFFSLSLHT-----TTAANPAVLDIDGHPLLSSSNYYILPVVRGRGGGLTVSPR 66
           +T+++ L F+ +  +       AAN  VLD DG  L +   YY+L  + G GGG     R
Sbjct: 4   ATAVVLLLFAFTSKSYFFGVANAANSPVLDTDGDELQTGVQYYVLSSISGAGGGGLALGR 63

Query: 67  NATELCPLNAAQDSREVEDGIPLELYPVNPNDRTVYVSTDLNLVFDAVP--LCVPSGPSF 126
              + CP    Q   ++++G P+     +  D  V VSTD+N+ F  +   LC     S 
Sbjct: 64  ATGQSCPEIVVQRRSDLDNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLC---STST 123

Query: 127 IWQL-TFDEATGRRYVGFGGQTGNPGIETVSNWFKIQKAGSGRFDYKIVFCPSVCNTCKV 186
           +W+L  +D + G+ +V   G  G PG  T+ +WFKI+KAG     YK  FCPSVC++C  
Sbjct: 124 VWRLDNYDNSAGKWWVTTDGVKGEPGPNTLCSWFKIEKAGV--LGYKFRFCPSVCDSCTT 183

Query: 187 MCGDVGVFVEGDGRRLLGIREQPLLVRFKRA 210
           +C D+G   + DG+  L + +      FK+A
Sbjct: 184 LCSDIGRHSDDDGQIRLALSDNEWAWMFKKA 209

[Close]

BLAST of Spo10770.1 vs. ExPASy Swiss-Prot
Match: IAAS_ORYSJ (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica GN=RASI PE=1 SV=2)

HSP 1 Score: 109.0 bits (271), Expect = 6.400e-23
Identity = 76/209 (36.36%), Postives = 108/209 (51.67%), Query Frame = 1


		  
Query: 5   ILSTSIIFLFFSLSLHTTTAANPAVLDIDGHPLLSSSNYYILPVVRGRGGGLTVSPRNAT 64
           ++  S++ + FS S     AA P V D +GH L +  +YY+LP   G GGGLT++PR   
Sbjct: 8   LILLSLLAISFSCS-----AAPPPVYDTEGHELSADGSYYVLPASPGHGGGLTMAPRVLP 67

Query: 65  ELCPLNAAQDSREVEDGIPLELYP----VNPNDRTVYVSTDLNLVFDAVPLCVPSGPSFI 124
             CPL  AQ++ E   G P+   P      P DRT+ VSTD+ + F+A  +CV S     
Sbjct: 68  --CPLLVAQETDERRKGFPVRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTE--- 127

Query: 125 WQLTFDEATGRRYVGFGGQTGNPGIETVSNWFKIQKAGSGRFDYKIVFCPSVCNTCKVMC 184
           W +  +  TG R V  G   G P      N F+++K G G   YK+V       +C+  C
Sbjct: 128 WHVGDEPLTGARRVVTGPLIG-PSPSGRENAFRVEKYGGG---YKLV-------SCRDSC 187

Query: 185 GDVGVFVEGDGRRLLGIREQPLLVRFKRA 210
            D+GV  +G  R  LG  + P +V FK+A
Sbjct: 188 QDLGVSRDG-ARAWLGASQPPHVVVFKKA 194

[Close]

BLAST of Spo10770.1 vs. ExPASy Swiss-Prot
Match: PIP22_PEA (Kunitz-type trypsin inhibitor-like 2 protein OS=Pisum sativum GN=PIP20-2 PE=3 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 1.600e-21
Identity = 64/191 (33.51%), Postives = 93/191 (48.69%), Query Frame = 1


		  
Query: 10  IIFLFFS-LSLHTTTAANPAVLDIDGHPLLSSSNYYILPVVRGRGGGLTVSPRNATELCP 69
           ++F+F + LSL  +      VLD++G P+     YYILP +RG  GG     R     CP
Sbjct: 12  LLFVFITTLSLAFSNEDVEQVLDVNGKPIFPGGQYYILPAIRGPPGGGVRLGRTGDLTCP 71

Query: 70  LNAAQDSREVEDGIPLELYPVNPNDRTVYVSTDLNLVFDAVPLCVPSGPSFIWQLTFDEA 129
           +   QD REV++G+P++      +   ++  T + + +   P C  S     W +  D  
Sbjct: 72  VTVLQDRREVKNGLPVKFVIPGISPGIIFTGTPIEIEYTKKPNCAKSSK---WLVFVDNV 131

Query: 130 TGRRYVGFGGQTGNPGIETVSNWFKIQKAGSGRFDYKIVFCPSVCNTCKVMCGDVGVFVE 189
             +  VG GG    PGI+T+S  FKI+K  SG F YK+ FC     TC     DVG F  
Sbjct: 132 IQKACVGIGGPENYPGIQTLSGLFKIEKHESG-FGYKLGFCIKGSPTCL----DVGRFDN 191

Query: 190 GDGRRLLGIRE 200
            +  R L + E
Sbjct: 192 DEAGRRLNLTE 194

[Close]

BLAST of Spo10770.1 vs. ExPASy Swiss-Prot
Match: DRTI_DELRE (Kunitz-type serine protease inhibitor DrTI OS=Delonix regia PE=1 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 1.600e-18
Identity = 52/161 (32.30%), Postives = 79/161 (49.07%), Query Frame = 1


		  
Query: 29  VLDIDGHPLLSSSNYYILPVVRGRGGGLTVSPRNATELCPLNAAQDSREVEDGIPLELYP 88
           V DI+G+P+   S YYI+  + G GGG     R    +CP++  Q+  +++ G+P+    
Sbjct: 6   VYDIEGYPVFLGSEYYIVSAIIGAGGGGVRPGRTRGSMCPMSIIQEQSDLQMGLPVRFSS 65

Query: 89  VNPNDRTVYVSTDLNLVFDAVPLCVPSGPSFIWQLTFDEATGRRYVGFGGQTGNPGIETV 148
              +   +Y  T+L + F   P C  S     W +  D  +G   V  GG   +P  E V
Sbjct: 66  PEESQGKIYTDTELEIEFVEKPDCAESSK---WVIVKD--SGEARVAIGGSEDHPQGELV 125

Query: 149 SNWFKIQKAGSGRFDYKIVFCPSVCNTCKVMCGDVGVFVEG 190
             +FKI+K GS    YK+VFCP    +    C D+G+  EG
Sbjct: 126 RGFFKIEKLGS--LAYKLVFCP---KSSSGSCSDIGINYEG 156

[Close]

BLAST of Spo10770.1 vs. TAIR (Arabidopsis)
Match: AT1G17860.1 (Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 174.5 bits (441), Expect = 6.900e-44
Identity = 100/209 (47.85%), Postives = 131/209 (62.68%), Query Frame = 1


		  
Query: 8   TSIIFLFFSLSLH------TTTAANPAVLDIDGHPLLSSSNYYILPVVRGRGGGLTVSPR 67
           +S++++F  L++       TT AA   V DI+G  LL+  NYYILPV+RGRGGGLT+S  
Sbjct: 2   SSLLYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMS-N 61

Query: 68  NATELCPLNAAQDSREVEDGIPLELYPVNPNDRTVYVSTDLNLVFDAVPLCVPSGPSFIW 127
             TE CP +  QD  EV  G+P++  P +   RT+ VSTD+N+ F          P+ IW
Sbjct: 62  LKTETCPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKF---------SPTSIW 121

Query: 128 QL-TFDEATGRRYVGFGGQTGNPGIETVSNWFKIQKAGSGRFDYKIVFCPSVCNTCKVMC 187
           +L  FDE T + ++   G  GNPG +TV NWFKI K      DYKI FCP+VCN CKV+C
Sbjct: 122 ELANFDETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEK---DYKIRFCPTVCNFCKVIC 181

Query: 188 GDVGVFVEGDGRRLLGIREQPLLVRFKRA 210
            DVGVFV+ DG+R L + + PL V FKRA
Sbjct: 182 RDVGVFVQ-DGKRRLALSDVPLKVMFKRA 195

[Close]

BLAST of Spo10770.1 vs. TAIR (Arabidopsis)
Match: AT1G73260.1 (kunitz trypsin inhibitor 1)

HSP 1 Score: 121.3 bits (303), Expect = 7.000e-28
Identity = 73/200 (36.50%), Postives = 115/200 (57.50%), Query Frame = 1


		  
Query: 12  FLFFSLSLHTTTAANPAVLDIDGHPLLSSSNYYILPVVRGRGGGLTVSPRNATELCPLNA 71
           +L  +L+    + A  AV+DIDG+ +   S YY+LPV+RGRGGGLT++ R   + CP + 
Sbjct: 13  YLVLALTAVLASNAYGAVVDIDGNAMFHES-YYVLPVIRGRGGGLTLAGRGG-QPCPYDI 72

Query: 72  AQDSREVEDGIPLELYPVNPNDRTVYVSTDLNLVFDA-VPLCVPSGPSFIWQL-TFDEAT 131
            Q+S EV++GIP++          V  S +LN+  D    +C+ S     W++  FD   
Sbjct: 73  VQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQST---YWRVGEFDHER 132

Query: 132 GRRYVGFGGQTGNPGIETVSNWFKIQKAGSGRFDYKIVFCPSVCNTCKVMCGDVGVFVEG 191
            + +V  G +    G +++ ++FKI+K  SG   YK VFCP  C++    C DVG+F++ 
Sbjct: 133 KQYFVVAGPKPEGFGQDSLKSFFKIEK--SGEDAYKFVFCPRTCDSGNPKCSDVGIFIDE 192

Query: 192 DGRRLLGIREQPLLVRFKRA 210
            G R L + ++P LV FK+A
Sbjct: 193 LGVRRLALSDKPFLVMFKKA 205

[Close]

BLAST of Spo10770.1 vs. TAIR (Arabidopsis)
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 99.0 bits (245), Expect = 3.700e-21
Identity = 66/213 (30.99%), Postives = 110/213 (51.64%), Query Frame = 1


		  
Query: 1   MTHFILSTSIIFLFFSLSLHTTTAANPAVLDIDGHPLLSSSNYYILPVVRGRGGGLTVSP 60
           +T   ++ +++   F+ +       +  VLDI GHP+ S+  YYI+P   G GGGL  S 
Sbjct: 4   LTLSFITLTVLSAIFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSN 63

Query: 61  RNAT--ELC-PLNAAQDSREVEDGIPLELYPVNPNDRTVYVSTDLNLVFDAVPLCVPSGP 120
           RN +  +LC  L+  Q S     G+P+   P+N   + V +S  LNL FD+     P   
Sbjct: 64  RNLSTQDLCLNLDIVQSSSPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPD-- 123

Query: 121 SFIWQLTFDEATGRRYVGFGGQTGNPGIETVSNWFKIQKAGSGRFDYKIVFCPSVCNTCK 180
           S +W++       + +V  GGQ G       ++WF+IQ+ G     YK+++CP    +  
Sbjct: 124 SKVWRIDHSVQLRKSFVSIGGQKGKG-----NSWFQIQEDGDA---YKLMYCPI---SSI 183

Query: 181 VMCGDVGVFVEGDG-RRLLGIREQPLLVRFKRA 210
           V C +V + ++  G RRL+   +Q  +V+F++A
Sbjct: 184 VACINVSLEIDDHGVRRLVLSTDQSFVVKFQKA 203

The following BLAST results are available for this feature:

BLAST of Spo10770.1 vs. NCBI nr
BLAST of Spo10770.1 vs. UniProtKB/TrEMBL
BLAST of Spo10770.1 vs. ExPASy Swiss-Prot
BLAST of Spo10770.1 vs. TAIR (Arabidopsis)

BLAST of Spo10770.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902082350\|gb\|KNA03166.1\|	6.8e-118	100.	hypothetical protein SOVF_2118...	[more]
gi\|902194163\|gb\|KNA12470.1\|	1.5e-77	66.5	hypothetical protein SOVF_1257...	[more]
gi\|731344031\|ref\|XP_010683193.1\|	7.9e-74	64.5	PREDICTED: miraculin-like [Bet...	[more]
gi\|731344033\|ref\|XP_010683194.1\|	1.8e-73	65.8	PREDICTED: miraculin-like [Bet...	[more]
gi\|902170260\|gb\|KNA07819.1\|	2.2e-63	57.5	hypothetical protein SOVF_1683...	[more]

BLAST of Spo10770.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9Q9I8_SPIOL	4.8e-118	100.	Uncharacterized protein OS=Spi...	[more]
A0A0K9QYY3_SPIOL	1.1e-77	66.5	Uncharacterized protein OS=Spi...	[more]
A0A0J8C0T9_BETVU	5.5e-74	64.5	Uncharacterized protein OS=Bet...	[more]
A0A0J8C4X4_BETVU	1.2e-73	65.8	Uncharacterized protein OS=Bet...	[more]
A0A0K9QKP3_SPIOL	1.5e-63	57.5	Uncharacterized protein OS=Spi...	[more]

BLAST of Spo10770.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
MIRA_SYNDU	1.5e-43	45.1	Miraculin OS=Synsepalum dulcif...	[more]
ASP_THECC	5.6e-27	33.6	21 kDa seed protein OS=Theobro...	[more]
IAAS_ORYSJ	6.4e-23	36.3	Alpha-amylase/subtilisin inhib...	[more]
PIP22_PEA	1.6e-21	33.5	Kunitz-type trypsin inhibitor-...	[more]
DRTI_DELRE	1.6e-18	32.3	Kunitz-type serine protease in...	[more]

BLAST of Spo10770.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 3


Match Name	E-value	Identity	Description
AT1G17860.1	6.9e-44	47.8	Kunitz family trypsin and prot...	[more]
AT1G73260.1	7.0e-28	36.5	kunitz trypsin inhibitor 1	[more]
AT1G73325.1	3.7e-21	30.9	Kunitz family trypsin and prot...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PRINTS	PR00291	KUNITZINHBTR	coord: 151..170 score: 2.5E-18coord: 67..87 score: 2.5E-18coord: 28..57 score: 2.5E-18coord: 177..206 score: 2.5
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 29..208 score: 9.0
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 28..209 score: 1.2
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 29..45 scor
IPR011065	Kunitz inhibitor ST1-like	unknown	SSF50386	STI-like	coord: 25..208 score: 3.36
None	No IPR available	GENE3D	2.80.10.50		coord: 25..209 score: 1.7
None	No IPR available	PANTHER	PTHR33107	FAMILY NOT NAMED	coord: 1..209 score: 2.6

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050832	defense response to fungus
biological_process	GO:0015824	proline transport
biological_process	GO:0006508	proteolysis
biological_process	GO:0009611	response to wounding
cellular_component	GO:0005618	cell wall
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004866	endopeptidase inhibitor activity
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo13051	0.68	Barchart \| Table
Spo02312	0.65	Barchart \| Table