Spo11370 (gene) | SpinachBase

Overview


Name	Spo11370
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein
Location	chr6 : 45491459 .. 45492109 (+)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAACACCAATATTTACTATCTTCCTCTTCCTTACCTCCATCCTTGCTATACTCTCCACAACCATAGCACACCACCCTCCAACTTCCAACCACTCTAAACCCAAACCCAAACCCGAACCCACTACTAACTTCATATGGTCATCTTGTAGTACAACAAGGTACCCTCCTCTTTGTTACAATGGCCTTTCACGCTATGCTAACAAGATAGGAAAAAGCCACCGCCAGGTGGCATTAACCGCCTTGGCGATGAGCTTATCCAGGGCCAGATCTGCTGCACGATTAGTTTCCCGGTTAAAAAAGGCTAAAGGGATAAAACATAGGGAATATGAAGCAGTTAAAGATTGTATTGATACCATGAGTGATGGGGTGGATCAATTGACCCGTTCGTTCGTGGAGCTTGCTAATTTGGGTCAAACCGATAGTGAGGAGTTCATGTGGCATATGGGTAATGTTCAAACATGGGTTAGTTCTGCTTTAACGGATGAGAGTACTTGTGTTGATGGGTTATCTGATGATGGATATAGTAATAATAATGGTGGGAGTAATAATAAGGTGAAAGGTGTTATTCAAATGAAGGTTAATAATGTTGCTCAACTTACTAGTAATGCTCTTGCTTTGGTTAATCGATTTGTAGAAAGGTATGGTTAA

mRNA sequence

ATGGCAACACCAATATTTACTATCTTCCTCTTCCTTACCTCCATCCTTGCTATACTCTCCACAACCATAGCACACCACCCTCCAACTTCCAACCACTCTAAACCCAAACCCAAACCCGAACCCACTACTAACTTCATATGGTCATCTTGTAGTACAACAAGGTACCCTCCTCTTTGTTACAATGGCCTTTCACGCTATGCTAACAAGATAGGAAAAAGCCACCGCCAGGTGGCATTAACCGCCTTGGCGATGAGCTTATCCAGGGCCAGATCTGCTGCACGATTAGTTTCCCGGTTAAAAAAGGCTAAAGGGATAAAACATAGGGAATATGAAGCAGTTAAAGATTGTATTGATACCATGAGTGATGGGGTGGATCAATTGACCCGTTCGTTCGTGGAGCTTGCTAATTTGGGTCAAACCGATAGTGAGGAGTTCATGTGGCATATGGGTAATGTTCAAACATGGGTTAGTTCTGCTTTAACGGATGAGAGTACTTGTGTTGATGGGTTATCTGATGATGGATATAGTAATAATAATGGTGGGAGTAATAATAAGGTGAAAGGTGTTATTCAAATGAAGGTTAATAATGTTGCTCAACTTACTAGTAATGCTCTTGCTTTGGTTAATCGATTTGTAGAAAGGTATGGTTAA

Coding sequence (CDS)

ATGGCAACACCAATATTTACTATCTTCCTCTTCCTTACCTCCATCCTTGCTATACTCTCCACAACCATAGCACACCACCCTCCAACTTCCAACCACTCTAAACCCAAACCCAAACCCGAACCCACTACTAACTTCATATGGTCATCTTGTAGTACAACAAGGTACCCTCCTCTTTGTTACAATGGCCTTTCACGCTATGCTAACAAGATAGGAAAAAGCCACCGCCAGGTGGCATTAACCGCCTTGGCGATGAGCTTATCCAGGGCCAGATCTGCTGCACGATTAGTTTCCCGGTTAAAAAAGGCTAAAGGGATAAAACATAGGGAATATGAAGCAGTTAAAGATTGTATTGATACCATGAGTGATGGGGTGGATCAATTGACCCGTTCGTTCGTGGAGCTTGCTAATTTGGGTCAAACCGATAGTGAGGAGTTCATGTGGCATATGGGTAATGTTCAAACATGGGTTAGTTCTGCTTTAACGGATGAGAGTACTTGTGTTGATGGGTTATCTGATGATGGATATAGTAATAATAATGGTGGGAGTAATAATAAGGTGAAAGGTGTTATTCAAATGAAGGTTAATAATGTTGCTCAACTTACTAGTAATGCTCTTGCTTTGGTTAATCGATTTGTAGAAAGGTATGGTTAA

Protein sequence

MATPIFTIFLFLTSILAILSTTIAHHPPTSNHSKPKPKPEPTTNFIWSSCSTTRYPPLCYNGLSRYANKIGKSHRQVALTALAMSLSRARSAARLVSRLKKAKGIKHREYEAVKDCIDTMSDGVDQLTRSFVELANLGQTDSEEFMWHMGNVQTWVSSALTDESTCVDGLSDDGYSNNNGGSNNKVKGVIQMKVNNVAQLTSNALALVNRFVERYG

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo11370.1	Spo11370.1	mRNA

Homology

[Close]

BLAST of Spo11370.1 vs. NCBI nr
Match: gi|902160135|gb|KNA06394.1| (hypothetical protein SOVF_181520 [Spinacia oleracea])

HSP 1 Score: 422.5 bits (1085), Expect = 4.300e-115
Identity = 216/216 (100.00%), Postives = 216/216 (100.00%), Query Frame = 1


		  
Query: 1   MATPIFTIFLFLTSILAILSTTIAHHPPTSNHSKPKPKPEPTTNFIWSSCSTTRYPPLCY 60
           MATPIFTIFLFLTSILAILSTTIAHHPPTSNHSKPKPKPEPTTNFIWSSCSTTRYPPLCY
Sbjct: 1   MATPIFTIFLFLTSILAILSTTIAHHPPTSNHSKPKPKPEPTTNFIWSSCSTTRYPPLCY 60

Query: 61  NGLSRYANKIGKSHRQVALTALAMSLSRARSAARLVSRLKKAKGIKHREYEAVKDCIDTM 120
           NGLSRYANKIGKSHRQVALTALAMSLSRARSAARLVSRLKKAKGIKHREYEAVKDCIDTM
Sbjct: 61  NGLSRYANKIGKSHRQVALTALAMSLSRARSAARLVSRLKKAKGIKHREYEAVKDCIDTM 120

Query: 121 SDGVDQLTRSFVELANLGQTDSEEFMWHMGNVQTWVSSALTDESTCVDGLSDDGYSNNNG 180
           SDGVDQLTRSFVELANLGQTDSEEFMWHMGNVQTWVSSALTDESTCVDGLSDDGYSNNNG
Sbjct: 121 SDGVDQLTRSFVELANLGQTDSEEFMWHMGNVQTWVSSALTDESTCVDGLSDDGYSNNNG 180

Query: 181 GSNNKVKGVIQMKVNNVAQLTSNALALVNRFVERYG 217
           GSNNKVKGVIQMKVNNVAQLTSNALALVNRFVERYG
Sbjct: 181 GSNNKVKGVIQMKVNNVAQLTSNALALVNRFVERYG 216

[Close]

BLAST of Spo11370.1 vs. NCBI nr
Match: gi|731330547|ref|XP_010676189.1| (PREDICTED: 21 kDa protein [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 277.7 bits (709), Expect = 1.700e-71
Identity = 153/215 (71.16%), Postives = 171/215 (79.53%), Query Frame = 1


		  
Query: 1   MAT-PIFTIFLFLTSILAILSTTIAHHPPTSNHSKPKPKPEPTTNFIWSSCSTTRYPPLC 60
           MAT P FT+F   +  ++ILSTTIAHHPP+S HSK       T+NFI SSC  T+YPPLC
Sbjct: 1   MATKPTFTLFFLFSLFISILSTTIAHHPPSSKHSKS------TSNFIRSSCLATQYPPLC 60

Query: 61  YNGLSRYANKIGKSHRQVALTALAMSLSRARSAARLVSRLKKAKGIKHREYEAVKDCIDT 120
           Y+ LSRYA+KIG+SHRQ+ALTALA SLSRARSAAR  SR+KK KGIKH E   VKDCIDT
Sbjct: 61  YHCLSRYASKIGRSHRQLALTALAASLSRARSAARFASRMKKTKGIKHTELVVVKDCIDT 120

Query: 121 MSDGVDQLTRSFVELANLGQTDSEEFMWHMGNVQTWVSSALTDESTCVDGLSDDGYSNNN 180
           MSDGVDQLTRSF ELAN G T+ E+FMWHM NVQTWVS+ALTD +TCVDGLSD GY    
Sbjct: 121 MSDGVDQLTRSFGELANSGYTNGEDFMWHMNNVQTWVSAALTDATTCVDGLSD-GY---- 180

Query: 181 GGSNNKVKGVIQMKVNNVAQLTSNALALVNRFVER 215
            G + KVKG IQ +VNNVAQLTSNALALVNRFV R
Sbjct: 181 -GKSGKVKGAIQRRVNNVAQLTSNALALVNRFVAR 203

[Close]

BLAST of Spo11370.1 vs. NCBI nr
Match: gi|470125365|ref|XP_004298673.1| (PREDICTED: 21 kDa protein [Fragaria vesca subsp. vesca])

HSP 1 Score: 179.5 bits (454), Expect = 6.300e-42
Identity = 96/179 (53.63%), Postives = 123/179 (68.72%), Query Frame = 1


		  
Query: 37  PKPEPTTNFIWSSCSTTRYPPLCYNGLSRYANKIGKSHRQVALTALAMSLSRARSAARLV 96
           P+   +T FI +SC  T+YP LC   L+ YA  I +S RQ+A TALA+SLSR R A   V
Sbjct: 27  PRHSSSTKFIKASCRATQYPALCVQCLAGYARSIRQSERQLAQTALAVSLSRVRKAESFV 86

Query: 97  SRLKKAKGIKHREYEAVKDCIDTMSDGVDQLTRSFVELANLGQTDSEEFMWHMGNVQTWV 156
           ++L K +GIK REY AVKDCI+ M DGVDQ+ +S  EL + G+   +EFMWHM NVQTWV
Sbjct: 87  TKLTKVRGIKPREYRAVKDCIENMGDGVDQIGQSMRELGHTGRAFGQEFMWHMSNVQTWV 146

Query: 157 SSALTDESTCVDGLSDDGYSNNNGGSNNKVKGVIQMKVNNVAQLTSNALALVNRFVERY 216
           S+ALTDE+TC+DG S      N       +K  I+ +VN+VAQ+TSNALALVNRF  ++
Sbjct: 147 SAALTDETTCLDGFSGRLMDGN-------IKVAIRRRVNDVAQVTSNALALVNRFATKH 198

[Close]

BLAST of Spo11370.1 vs. NCBI nr
Match: gi|1009157058|ref|XP_015896569.1| (PREDICTED: 21 kDa protein-like [Ziziphus jujuba])

HSP 1 Score: 178.7 bits (452), Expect = 1.100e-41
Identity = 101/211 (47.87%), Postives = 136/211 (64.45%), Query Frame = 1


		  
Query: 5   IFTIFLFLTSILAILSTTIAHHPPTSNHSKPKPKPEPTTNFIWSSCSTTRYPPLCYNGLS 64
           +  +FLFL  +  +  + IA HP +S+             F+ SSC  T YP LC+  L+
Sbjct: 8   LVVLFLFLY-VFGMAESAIARHPTSSD-------------FVKSSCRVTHYPALCFQSLA 67

Query: 65  RYANKIGKSHRQVALTALAMSLSRARSAARLVSRLKKAKGIKHREYEAVKDCIDTMSDGV 124
            YA  I +S RQ+A TAL +SL+R RS+   +++L K +GIK REY AVKDCI+TMSD V
Sbjct: 68  GYAKAIQQSERQLAQTALTVSLARVRSSTAFLAKLTKVRGIKPREYRAVKDCIETMSDSV 127

Query: 125 DQLTRSFVELANLGQTDSEEFMWHMGNVQTWVSSALTDESTCVDGLSDDGYSNNNGGSNN 184
           DQL+ S  EL ++G+   ++F+WH+ NVQTWVSSALTDE+TC+DG S      N      
Sbjct: 128 DQLSESVRELGHMGRAVGQDFLWHVSNVQTWVSSALTDENTCLDGFSARFMDGN------ 187

Query: 185 KVKGVIQMKVNNVAQLTSNALALVNRFVERY 216
            VK  I+ +V NVAQ+TSNALALVNRF  ++
Sbjct: 188 -VKTAIRRRVVNVAQVTSNALALVNRFASKH 197

[Close]

BLAST of Spo11370.1 vs. NCBI nr
Match: gi|731438694|ref|XP_010646817.1| (PREDICTED: 21 kDa protein [Vitis vinifera])

HSP 1 Score: 178.3 bits (451), Expect = 1.400e-41
Identity = 95/172 (55.23%), Postives = 124/172 (72.09%), Query Frame = 1


		  
Query: 43  TNFIWSSCSTTRYPPLCYNGLSRYANKIGKSHRQVALTALAMSLSRARSAARLVSRLKKA 102
           T+FI +SC  TRYP LC+  LS YA+ I +S RQ+ALTAL++SLSRARSA    S+L K 
Sbjct: 32  TSFIKASCRVTRYPVLCFQCLSGYASTIRQSDRQLALTALSVSLSRARSATVFASKLTKV 91

Query: 103 KGIKHREYEAVKDCIDTMSDGVDQLTRSFVELANLGQTDSEEFMWHMGNVQTWVSSALTD 162
           +G+K RE+EAVKDCI+ M+D VD+L+ S  EL   G+  S++FMWHM NVQTWVS+ALTD
Sbjct: 92  RGLKPREHEAVKDCIENMADTVDRLSHSVQELGRTGKAVSQDFMWHMSNVQTWVSAALTD 151

Query: 163 ESTCVDGLSDDGYSNNNGGSNNKVKGVIQMKVNNVAQLTSNALALVNRFVER 215
           E+TC+DG +      N       VK  ++ KV +VAQ+TSNALAL+NRF  +
Sbjct: 152 ENTCLDGFAGRVMEGN-------VKTAVRRKVIDVAQVTSNALALINRFAAK 196

[Close]

BLAST of Spo11370.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QGG9_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_181520 PE=4 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 3.000e-115
Identity = 216/216 (100.00%), Postives = 216/216 (100.00%), Query Frame = 1


		  
Query: 1   MATPIFTIFLFLTSILAILSTTIAHHPPTSNHSKPKPKPEPTTNFIWSSCSTTRYPPLCY 60
           MATPIFTIFLFLTSILAILSTTIAHHPPTSNHSKPKPKPEPTTNFIWSSCSTTRYPPLCY
Sbjct: 1   MATPIFTIFLFLTSILAILSTTIAHHPPTSNHSKPKPKPEPTTNFIWSSCSTTRYPPLCY 60

Query: 61  NGLSRYANKIGKSHRQVALTALAMSLSRARSAARLVSRLKKAKGIKHREYEAVKDCIDTM 120
           NGLSRYANKIGKSHRQVALTALAMSLSRARSAARLVSRLKKAKGIKHREYEAVKDCIDTM
Sbjct: 61  NGLSRYANKIGKSHRQVALTALAMSLSRARSAARLVSRLKKAKGIKHREYEAVKDCIDTM 120

Query: 121 SDGVDQLTRSFVELANLGQTDSEEFMWHMGNVQTWVSSALTDESTCVDGLSDDGYSNNNG 180
           SDGVDQLTRSFVELANLGQTDSEEFMWHMGNVQTWVSSALTDESTCVDGLSDDGYSNNNG
Sbjct: 121 SDGVDQLTRSFVELANLGQTDSEEFMWHMGNVQTWVSSALTDESTCVDGLSDDGYSNNNG 180

Query: 181 GSNNKVKGVIQMKVNNVAQLTSNALALVNRFVERYG 217
           GSNNKVKGVIQMKVNNVAQLTSNALALVNRFVERYG
Sbjct: 181 GSNNKVKGVIQMKVNNVAQLTSNALALVNRFVERYG 216

[Close]

BLAST of Spo11370.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CKF3_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_5g098090 PE=4 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 1.200e-71
Identity = 153/215 (71.16%), Postives = 171/215 (79.53%), Query Frame = 1


		  
Query: 1   MAT-PIFTIFLFLTSILAILSTTIAHHPPTSNHSKPKPKPEPTTNFIWSSCSTTRYPPLC 60
           MAT P FT+F   +  ++ILSTTIAHHPP+S HSK       T+NFI SSC  T+YPPLC
Sbjct: 1   MATKPTFTLFFLFSLFISILSTTIAHHPPSSKHSKS------TSNFIRSSCLATQYPPLC 60

Query: 61  YNGLSRYANKIGKSHRQVALTALAMSLSRARSAARLVSRLKKAKGIKHREYEAVKDCIDT 120
           Y+ LSRYA+KIG+SHRQ+ALTALA SLSRARSAAR  SR+KK KGIKH E   VKDCIDT
Sbjct: 61  YHCLSRYASKIGRSHRQLALTALAASLSRARSAARFASRMKKTKGIKHTELVVVKDCIDT 120

Query: 121 MSDGVDQLTRSFVELANLGQTDSEEFMWHMGNVQTWVSSALTDESTCVDGLSDDGYSNNN 180
           MSDGVDQLTRSF ELAN G T+ E+FMWHM NVQTWVS+ALTD +TCVDGLSD GY    
Sbjct: 121 MSDGVDQLTRSFGELANSGYTNGEDFMWHMNNVQTWVSAALTDATTCVDGLSD-GY---- 180

Query: 181 GGSNNKVKGVIQMKVNNVAQLTSNALALVNRFVER 215
            G + KVKG IQ +VNNVAQLTSNALALVNRFV R
Sbjct: 181 -GKSGKVKGAIQRRVNNVAQLTSNALALVNRFVAR 203

[Close]

BLAST of Spo11370.1 vs. UniProtKB/TrEMBL
Match: F6HHR9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_00s0323g00050 PE=4 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 9.800e-42
Identity = 95/172 (55.23%), Postives = 124/172 (72.09%), Query Frame = 1


		  
Query: 43  TNFIWSSCSTTRYPPLCYNGLSRYANKIGKSHRQVALTALAMSLSRARSAARLVSRLKKA 102
           T+FI +SC  TRYP LC+  LS YA+ I +S RQ+ALTAL++SLSRARSA    S+L K 
Sbjct: 32  TSFIKASCRVTRYPVLCFQCLSGYASTIRQSDRQLALTALSVSLSRARSATVFASKLTKV 91

Query: 103 KGIKHREYEAVKDCIDTMSDGVDQLTRSFVELANLGQTDSEEFMWHMGNVQTWVSSALTD 162
           +G+K RE+EAVKDCI+ M+D VD+L+ S  EL   G+  S++FMWHM NVQTWVS+ALTD
Sbjct: 92  RGLKPREHEAVKDCIENMADTVDRLSHSVQELGRTGKAVSQDFMWHMSNVQTWVSAALTD 151

Query: 163 ESTCVDGLSDDGYSNNNGGSNNKVKGVIQMKVNNVAQLTSNALALVNRFVER 215
           E+TC+DG +      N       VK  ++ KV +VAQ+TSNALAL+NRF  +
Sbjct: 152 ENTCLDGFAGRVMEGN-------VKTAVRRKVIDVAQVTSNALALINRFAAK 196

[Close]

BLAST of Spo11370.1 vs. UniProtKB/TrEMBL
Match: A0A061G797_THECC (Plant invertase/pectin methylesterase inhibitor superfamily protein OS=Theobroma cacao GN=TCM_014952 PE=4 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 9.800e-42
Identity = 92/182 (50.55%), Postives = 128/182 (70.33%), Query Frame = 1


		  
Query: 33  SKPKPKPEPTTNFIWSSCSTTRYPPLCYNGLSRYANKIGKSHRQVALTALAMSLSRARSA 92
           + P       +NFI +SCS T+YP LC   L+ +A  I +S RQ+A TAL +SL+RA+S 
Sbjct: 55  TSPAAASSRASNFIKASCSATKYPALCVQSLAAFAPSIQQSPRQLAQTALNVSLARAQST 114

Query: 93  ARLVSRLKKAKGIKHREYEAVKDCIDTMSDGVDQLTRSFVELANLGQTDSEEFMWHMGNV 152
              VS++KK KG++ REYEA+KDCI+ MSD VD+L++S  EL ++GQ   ++F+WH+ NV
Sbjct: 115 EAFVSKMKKFKGLEKREYEAIKDCIEEMSDSVDRLSKSVQELKHMGQAKGQDFLWHVSNV 174

Query: 153 QTWVSSALTDESTCVDGLSDDGYSNNNGGSNNKVKGVIQMKVNNVAQLTSNALALVNRFV 212
           +TWVS+ALTDESTCVDG +  G        + KVK  I+ +V NVAQ+TSNAL+LVN+F 
Sbjct: 175 ETWVSAALTDESTCVDGFASQGL-------DGKVKASIRARVMNVAQVTSNALSLVNQFA 229

Query: 213 ER 215
            +
Sbjct: 235 SK 229

[Close]

BLAST of Spo11370.1 vs. UniProtKB/TrEMBL
Match: W9QU28_9ROSA (21 kDa protein OS=Morus notabilis GN=L484_018999 PE=4 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 1.700e-41
Identity = 104/206 (50.49%), Postives = 135/206 (65.53%), Query Frame = 1


		  
Query: 10  LFLTSILAILSTTIAHHPPTSNHSKPKPKPEPTTNFIWSSCSTTRYPPLCYNGLSRYANK 69
           L  T ++ I++  +A    T + S       PT NFI +SC TT YP LC   LS +A +
Sbjct: 9   LLSTLVVLIMAANVAKSTNTISRSVNTKSSTPT-NFIRASCKTTSYPALCVQCLSAFAKQ 68

Query: 70  IGKSHRQVALTALAMSLSRARSAARLVSRLKKAKGIKHREYEAVKDCIDTMSDGVDQLTR 129
           I +S RQ+A TAL +SLSRARSA+ LVS++ + +GIK REY+AVKDCI+ M D V+ L+ 
Sbjct: 69  IRRSERQLAQTALTVSLSRARSASHLVSKMTRVRGIKPREYQAVKDCIENMGDTVNTLSH 128

Query: 130 SFVELANLGQTDSEEFMWHMGNVQTWVSSALTDESTCVDGLSDDGYSNNNGGSNNKVKGV 189
           S  EL  +GQ D   F+WHM NVQTWVS+ALTDE+TC+DG S      N       VK  
Sbjct: 129 SVRELGQVGQQD---FVWHMSNVQTWVSAALTDENTCLDGFSGRFMDGN-------VKAA 188

Query: 190 IQMKVNNVAQLTSNALALVNRFVERY 216
           I+ +V NVAQ+TSNALALVNRF  ++
Sbjct: 189 IRRRVVNVAQVTSNALALVNRFASKH 203

[Close]

BLAST of Spo11370.1 vs. ExPASy Swiss-Prot
Match: 21KD_DAUCA (21 kDa protein OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 2.700e-32
Identity = 73/175 (41.71%), Postives = 111/175 (63.43%), Query Frame = 1


		  
Query: 41  PTTNFIWSSCSTTRYPPLCYNGLSRYANKIGKSHRQVALTALAMSLSRARSAARLVSRLK 100
           P   FI +SC+ T YP +C   LS YA  I  + +++A TAL +SL+R + A   + RL 
Sbjct: 24  PANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTALQVSLTRTQQAQTFMKRLN 83

Query: 101 KAKGIKHREYEAVKDCIDTMSDGVDQLTRSFVELANLGQTDSEEFMWHMGNVQTWVSSAL 160
           K KG+K R+Y A+ DC++ + D +D+++RS  E+ NL      +F + M NV+TWVS+AL
Sbjct: 84  KFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKGNDFTFRMSNVETWVSAAL 143

Query: 161 TDESTCVDGLSDDGYSNNNGGSNNKVKGVIQMKVNNVAQLTSNALALVNRFVERY 216
           TDE+TC+DG +         G + K+K  ++ +V  VA++TSNALALVN F  ++
Sbjct: 144 TDETTCMDGFA-------GKGMDGKIKESVRAQVVAVARVTSNALALVNNFAAKH 191

[Close]

BLAST of Spo11370.1 vs. ExPASy Swiss-Prot
Match: PME3_ARATH (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 SV=2)

HSP 1 Score: 70.1 bits (170), Expect = 3.400e-11
Identity = 50/167 (29.94%), Postives = 90/167 (53.89%), Query Frame = 1


		  
Query: 48  SSCSTTRYPPLCYNGLSRYANKIGKSHRQVALTALAMSLSRARSAARLVSRL-KKAKGIK 107
           SSCS+TRYP LC + +         S + V   ++ ++++        V +L KK KG+ 
Sbjct: 61  SSCSSTRYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLT 120

Query: 108 HREYEAVKDCIDTMSDGVDQLTRSFVELANLGQTDSEEFMWHMGNVQTWVSSALTDESTC 167
            RE  A+ DC++T+ + +D+L  + VE  +L  T  +    H G+++T +SSA+T++ TC
Sbjct: 121 PREKTALHDCLETIDETLDELHET-VEDLHLYPT-KKTLREHAGDLKTLISSAITNQETC 180

Query: 168 VDGLSDDGYSNNNGGSNNKVKGVIQMKVNNVAQLTSNALALVNRFVE 214
           +DG S D        ++ +V+  +     +V  + SNALA++    +
Sbjct: 181 LDGFSHD-------DADKQVRKALLKGQIHVEHMCSNALAMIKNMTD 218

[Close]

BLAST of Spo11370.1 vs. ExPASy Swiss-Prot
Match: PMEU1_SOLLC (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 5.800e-11
Identity = 53/203 (26.11%), Postives = 106/203 (52.22%), Query Frame = 1


		  
Query: 10  LFLTSILAILSTTIAHHPPTSNHSKPKPKPEPTTNFIWSSCSTTRYPPLCYNGL---SRY 69
           L + +++ +++   +H   + +H+            + S+CS T +P LCY+ +   S +
Sbjct: 30  LLVAAVIGVVAGVKSHSKNSDDHADIMAISSSAHAIVKSACSNTLHPELCYSAIVNVSDF 89

Query: 70  ANKIGKSHRQVALTALAMSLSRARSAARLVSRL-KKAKGIKHREYEAVKDCIDTMSDGVD 129
           + K+  S + V   +L +++   R     V  L K  KG+  RE  A+ DC++TM + +D
Sbjct: 90  SKKV-TSQKDVIELSLNITVKAVRRNYYAVKELIKTRKGLTPREKVALHDCLETMDETLD 149

Query: 130 QLTRSFVELANLGQTDSEEFMWHMGNVQTWVSSALTDESTCVDGLSDDGYSNNNGGSNNK 189
           +L  +  +L      + +    H+ +++T +SSA+T++ TC+DG S D        ++ K
Sbjct: 150 ELHTAVEDLELY--PNKKSLKEHVEDLKTLISSAITNQETCLDGFSHD-------EADKK 209

Query: 190 VKGVIQMKVNNVAQLTSNALALV 209
           V+ V+     +V ++ SNALA++
Sbjct: 210 VRKVLLKGQKHVEKMCSNALAMI 222

[Close]

BLAST of Spo11370.1 vs. ExPASy Swiss-Prot
Match: PME2_ARATH (Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2)

HSP 1 Score: 68.6 bits (166), Expect = 9.800e-11
Identity = 59/208 (28.37%), Postives = 107/208 (51.44%), Query Frame = 1


		  
Query: 10  LFLTSILAILSTTIAHHPPTSNHSKPKPKPEPTTN--FIWSSCSTTRYPPLCYNGLSRYA 69
           L L SI+ I +TT      T+ +   K     +T+   + S CS+T YP LC++ ++   
Sbjct: 30  LLLASIVGIAATT------TNQNKNQKITTLSSTSHAILKSVCSSTLYPELCFSAVAATG 89

Query: 70  NKIGKSHRQVALTALAMSLSRARSAARLVSRL-KKAKGIKHREYEAVKDCIDTMSDGVDQ 129
            K   S ++V   +L ++    +     V +L  K KG+  RE  A+ DC++T+ + +D+
Sbjct: 90  GKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHDCLETIDETLDE 149

Query: 130 LTRSFVELANLGQTDSEEFMW-HMGNVQTWVSSALTDESTCVDGLSDDGYSNNNGGSNNK 189
           L    V + +L Q   ++ +  H  +++T +SSA+T++ TC+DG S D        ++ K
Sbjct: 150 L---HVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYD-------DADRK 209

Query: 190 VKGVIQMKVNNVAQLTSNALALVNRFVE 214
           V+  +     +V  + SNALA++    E
Sbjct: 210 VRKALLKGQVHVEHMCSNALAMIKNMTE 221

[Close]

BLAST of Spo11370.1 vs. ExPASy Swiss-Prot
Match: PME3_CITSI (Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 8.300e-10
Identity = 51/211 (24.17%), Postives = 105/211 (49.76%), Query Frame = 1


		  
Query: 5   IFTIFLFLTSILAILSTTIAHHPPTSNHSKPKPKPEPTTNFIWSSCSTTRYPPLCYNGLS 64
           +F   L + +++ I++   +      N +      EP    + SSCS+TRYP LC++ ++
Sbjct: 34  LFATLLVVAAVIGIVAGVNSRKNSGDNGN------EPHHAILKSSCSSTRYPDLCFSAIA 93

Query: 65  RY--ANKIGKSHRQVALTALAMSLSRARSAARLVSRLKKAKGIKHREYEAVKDCIDTMSD 124
               A+K   S + V   +L ++ +        + +L K   +  RE  A+ DC++T+ +
Sbjct: 94  AVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKVALHDCLETIDE 153

Query: 125 GVDQLTRSFVELANLGQTDSEEFMWHMGNVQTWVSSALTDESTCVDGLSDDGYSNNNGGS 184
            +D+L ++  +L      + +    H  +++T +S+A+T++ TC+DG S D        +
Sbjct: 154 TLDELHKAVEDLEEY--PNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHD-------DA 213

Query: 185 NNKVKGVIQMKVNNVAQLTSNALALVNRFVE 214
           N  V+  +     +V ++ SNALA++    +
Sbjct: 214 NKHVRDALSDGQVHVEKMCSNALAMIKNMTD 229

[Close]

BLAST of Spo11370.1 vs. TAIR (Arabidopsis)
Match: AT4G12390.1 (pectin methylesterase inhibitor 1)

HSP 1 Score: 155.2 bits (391), Expect = 4.500e-38
Identity = 89/190 (46.84%), Postives = 126/190 (66.32%), Query Frame = 1


		  
Query: 30  SNHSKPKPKPEPTT--NFIWSSCSTTRYPPLCYNGLSRYANKIGKSHRQVALTALAMSLS 89
           S  +KP   P P++  NFI SSC  TRY  LC   L+ +A+KI ++  Q+A TALA++L 
Sbjct: 22  STIAKPSSSPNPSSSINFIVSSCRVTRYQTLCVKCLAAFADKIRRNENQLAQTALAVTLV 81

Query: 90  RARSAARLVSRLKKAKGIKHREYEAVKDCIDTMSDGVDQLTRSFVELANLGQT--DSEEF 149
           R +S    V +L KA+ IK REY AVKDC++ + DG++ L +S  EL  +G++  D +EF
Sbjct: 82  RVQSTTIYVGKLTKARRIKRREYLAVKDCVENLGDGLEMLAQSMRELKQVGRSGRDRDEF 141

Query: 150 MWHMGNVQTWVSSALTDESTCVDGLSDDGYSNNNGGSNNKVKGVIQMKVNNVAQLTSNAL 209
           +W + NV+TWVS+ALTDE+TC+DG   DG        +  VK  I+ +V +VA++TSNAL
Sbjct: 142 LWRLSNVETWVSAALTDETTCLDGF--DGKV-----MDGVVKSAIRRRVVHVARVTSNAL 201

Query: 210 ALVNRFVERY 216
           ALVNRF  R+
Sbjct: 202 ALVNRFAARH 204

[Close]

BLAST of Spo11370.1 vs. TAIR (Arabidopsis)
Match: AT1G62770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 153.7 bits (387), Expect = 1.300e-37
Identity = 102/211 (48.34%), Postives = 136/211 (64.45%), Query Frame = 1


		  
Query: 7   TIFLFLTSILAILSTTIAHHPPTSNHSKPKPKPEPTTNFIWSSCSTTRYPPLCYNGLSRY 66
           TIFL +   + IL ++ A    T N S+       +  FI SSC TT+YP LC + LS Y
Sbjct: 6   TIFLVILLSITILQSSSA----TPNRSE-------SDQFIVSSCQTTQYPSLCVHTLSAY 65

Query: 67  ANKIGKSHRQ-VALTALAMSLSRARSAARLVSRL-KKAKGIKHREYEAVKDCIDTMSDGV 126
           A KI  ++ Q +A TAL +SL+RA+S    V++L K+    K REY A+KDCI+ + + V
Sbjct: 66  ATKIRHNNDQDLAQTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSV 125

Query: 127 DQLTRSFVELANLGQT-DSEEFMWHMGNVQTWVSSALTDESTCVDGLSDDGYSNNNGGSN 186
           D+L +S  ELA  G    SE+FMW M NVQTWVS+ALTDE+TC+DG S+       GG  
Sbjct: 126 DRLAQSVKELARAGHAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAM----GG-- 185

Query: 187 NKVKGVIQMKVNNVAQLTSNALALVNRFVER 215
            KVK +I+ KV +VAQ+TSNALALVN+F E+
Sbjct: 186 -KVKRLIRYKVVHVAQVTSNALALVNQFAEK 198

[Close]

BLAST of Spo11370.1 vs. TAIR (Arabidopsis)
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 153.7 bits (387), Expect = 1.300e-37
Identity = 83/173 (47.98%), Postives = 114/173 (65.90%), Query Frame = 1


		  
Query: 44  NFIWSSCSTTRYPPLCYNGLSRYANKIGKSHRQVALTALAMSLSRARSAARLVSRLKKAK 103
           NFI SSC TT YP LC + LS YAN I  S +++A TA+A++LSRA+S    VSRL + K
Sbjct: 37  NFIQSSCKTTTYPALCVHSLSVYANDIQTSPKRLAETAIAVTLSRAQSTKLFVSRLTRMK 96

Query: 104 GIKHREYEAVKDCIDTMSDGVDQLTRSFVELANLGQT-DSEEFMWHMGNVQTWVSSALTD 163
           G+K RE EA+KDC++ M+D VD+LT+S  EL   G   D ++F +HM N QTW S+ALTD
Sbjct: 97  GLKKREVEAIKDCVEEMNDTVDRLTKSVQELKLCGSAKDQDQFAYHMSNAQTWTSAALTD 156

Query: 164 ESTCVDGLSDDGYSNNNGGSNNKVKGVIQMKVNNVAQLTSNALALVNRFVERY 216
           E+TC DG S           + ++K  ++ ++ NV   TSNAL+L+N F + Y
Sbjct: 157 ENTCSDGFS-------GRVMDGRIKNSVRARIMNVGHETSNALSLINAFAKTY 202

[Close]

BLAST of Spo11370.1 vs. TAIR (Arabidopsis)
Match: AT4G25260.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 139.4 bits (350), Expect = 2.600e-33
Identity = 77/174 (44.25%), Postives = 114/174 (65.52%), Query Frame = 1


		  
Query: 43  TNFIWSSCSTTRYPPLCYNGLSRYANKIGKSHRQVALTALAMSLSRARSAARLVSRLKKA 102
           T FI +SC TT YP  C+  LS YA++I K  R++A TALA+S++RA+SA   VS +   
Sbjct: 35  TEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKLAETALAVSIARAKSAKTYVSEMTDY 94

Query: 103 KGIKHREYEAVKDCIDTMSDGVDQLTRSFVELANLGQTDS-EEFMWHMGNVQTWVSSALT 162
           KGI  R++EAV DC++ M D VD+L+ S  EL +L + DS E+F + + NV+TW S+ALT
Sbjct: 95  KGITKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEEGDSGEDFWFCLSNVRTWTSAALT 154

Query: 163 DESTCVDGLSDDGYSNNNGGSNNKVKGVIQMKVNNVAQLTSNALALVNRFVERY 216
           DE+ C+DG      +        ++K +I+  + +VA+ TSNALAL+N F  ++
Sbjct: 155 DETACMDGFGGKAMA-------GELKSLIRTHIVSVAEETSNALALINDFASKH 201

[Close]

BLAST of Spo11370.1 vs. TAIR (Arabidopsis)
Match: AT3G47380.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 127.9 bits (320), Expect = 7.700e-30
Identity = 87/219 (39.73%), Postives = 128/219 (58.45%), Query Frame = 1


		  
Query: 1   MATPIF-TIFLFLTSILAILSTTIAHHPPTSNHSKPKPK--PEPTTNFIWSSCSTTRYPP 60
           MA  IF T+FLFL      LST I     T++ S P+     +   NFI +SC  T YP 
Sbjct: 1   MAKQIFYTLFLFL------LSTAIL----TASSSAPRAAITSKRAINFIQASCKATTYPT 60

Query: 61  LCYNGLSRYANKIGKSHRQVALTALAMSLSRARSAARLVSRLKKAKGIKHREYEAVKDCI 120
           +C N L+ YAN I  S R++A TAL +++++A+S    V RL +   +K RE +AVKDCI
Sbjct: 61  VCVNSLTGYANSIQTSPRRLAETALNVTVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCI 120

Query: 121 DTMSDGVDQLTRSFVELANLGQTDS-EEFMWHMGNVQTWVSSALTDESTCVDGLSDDGYS 180
           + + D VD+LT S  E+   G     ++F +HM N QTW S+ALT+ +TC DG +     
Sbjct: 121 EEIHDAVDRLTMSIHEVKMCGSAKGRDQFWFHMSNAQTWTSAALTNANTCSDGFA----- 180

Query: 181 NNNGGSNNKVKGVIQMKVNNVAQLTSNALALVNRFVERY 216
                 + +VK  ++ ++ N+ + TSNALAL+N F ++Y
Sbjct: 181 --GRVMDGRVKNSVRARILNLGRGTSNALALINAFAKKY 202

The following BLAST results are available for this feature:

BLAST of Spo11370.1 vs. NCBI nr
BLAST of Spo11370.1 vs. UniProtKB/TrEMBL
BLAST of Spo11370.1 vs. ExPASy Swiss-Prot
BLAST of Spo11370.1 vs. TAIR (Arabidopsis)

BLAST of Spo11370.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902160135\|gb\|KNA06394.1\|	4.3e-115	100.	hypothetical protein SOVF_1815...	[more]
gi\|731330547\|ref\|XP_010676189.1\|	1.7e-71	71.1	PREDICTED: 21 kDa protein [Bet...	[more]
gi\|470125365\|ref\|XP_004298673.1\|	6.3e-42	53.6	PREDICTED: 21 kDa protein [Fra...	[more]
gi\|1009157058\|ref\|XP_015896569.1\|	1.1e-41	47.8	PREDICTED: 21 kDa protein-like...	[more]
gi\|731438694\|ref\|XP_010646817.1\|	1.4e-41	55.2	PREDICTED: 21 kDa protein [Vit...	[more]

BLAST of Spo11370.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9QGG9_SPIOL	3.0e-115	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8CKF3_BETVU	1.2e-71	71.1	Uncharacterized protein OS=Bet...	[more]
F6HHR9_VITVI	9.8e-42	55.2	Putative uncharacterized prote...	[more]
A0A061G797_THECC	9.8e-42	50.5	Plant invertase/pectin methyle...	[more]
W9QU28_9ROSA	1.7e-41	50.4	21 kDa protein OS=Morus notabi...	[more]

BLAST of Spo11370.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
21KD_DAUCA	2.7e-32	41.7	21 kDa protein OS=Daucus carot...	[more]
PME3_ARATH	3.4e-11	29.9	Pectinesterase/pectinesterase ...	[more]
PMEU1_SOLLC	5.8e-11	26.1	Pectinesterase/pectinesterase ...	[more]
PME2_ARATH	9.8e-11	28.3	Pectinesterase 2 OS=Arabidopsi...	[more]
PME3_CITSI	8.3e-10	24.1	Pectinesterase 3 OS=Citrus sin...	[more]

BLAST of Spo11370.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT4G12390.1	4.5e-38	46.8	pectin methylesterase inhibito...	[more]
AT1G62770.1	1.3e-37	48.3	Plant invertase/pectin methyle...	[more]
AT5G62350.1	1.3e-37	47.9	Plant invertase/pectin methyle...	[more]
AT4G25260.1	2.6e-33	44.2	Plant invertase/pectin methyle...	[more]
AT3G47380.1	7.7e-30	39.7	Plant invertase/pectin methyle...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	GENE3D	1.20.140.40		coord: 42..211 score: 7.3
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 45..207 score: 2.4
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 41..207 score: 5.0
IPR006501	Pectinesterase inhibitor domain	TIGRFAMs	TIGR01614	TIGR01614	coord: 20..209 score: 6.2
IPR006501	Pectinesterase inhibitor domain	unknown	SSF101148	Plant invertase/pectin methylesterase inhibitor	coord: 44..211 score: 3.79
None	No IPR available	PANTHER	PTHR31080	FAMILY NOT NAMED	coord: 5..215 score: 1.3
None	No IPR available	PANTHER	PTHR31080:SF2	F23N19.14-RELATED	coord: 5..215 score: 1.3

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo03903	0.68	Barchart \| Table