Spo11449 (gene)

BLAST of Spo11449.1 vs. NCBI nr
Match: gi|902235779|gb|KNA24082.1| (hypothetical protein SOVF_018370 isoform A [Spinacia oleracea])

HSP 1 Score: 954.5 bits (2466), Expect = 1.000e-274
Identity = 482/524 (91.98%), Postives = 488/524 (93.13%), Query Frame = 1

		  

Query: 87  MEKQKQKPSKPIKEILWFSLFSTSIKLLLLPSYRSTDFEVHRNWLATTHSLPLSHWYSDT 146
           MEKQKQKPSKPIKEILWFSLFSTSIKLLLLPSYRSTDFEVHRNWLATTHSLPLSHWYSDT
Sbjct: 1   MEKQKQKPSKPIKEILWFSLFSTSIKLLLLPSYRSTDFEVHRNWLATTHSLPLSHWYSDT 60

Query: 147 TSQWTLDYPPFFAYFELFLSFIAAQIDPAIVNLHEGLNYKADSVIYFQRITVIVSDLILH 206
           TSQWTLDYPPFFAYFELFLSFIAAQIDPAIVNLHEGLNYKADSVIYFQRITVIVSDLILH
Sbjct: 61  TSQWTLDYPPFFAYFELFLSFIAAQIDPAIVNLHEGLNYKADSVIYFQRITVIVSDLILH 120

Query: 207 YAIYRITRNLDSTKRRLIWILVIWSPALLIVDHMHFQYNGFLLGFLLISISALIEGRDLM 266
           YAIYRITRNLDSTKRRLIWILVIWSPALLIVDHMHFQYNGFLLGFLLISISALIEGRDLM
Sbjct: 121 YAIYRITRNLDSTKRRLIWILVIWSPALLIVDHMHFQYNGFLLGFLLISISALIEGRDLM 180

Query: 267 GGFFFAVLLCFKHLFAVAAPVYFVYLLRHYCRGGVQHTPGSHQMMLRALMQNSSACM--- 326
           GGFFFAVLLCFKHLFAVAAPVYFVYLLRHYCRGG+    G   +M  A++    A     
Sbjct: 181 GGFFFAVLLCFKHLFAVAAPVYFVYLLRHYCRGGLFKGFGRLLLMGLAVVVVFVAAFWPF 240

Query: 327 ----KMLQVIHRMFPFGRGLCHAFWAPNFWVFYIMADKLLAFFLRRMGFNVQRPAGSFTS 386
               +MLQVIHRMFPFGRGLCHAFWAPNFWVFYIMADKLLAFFLRRMGFNVQRPAGSFTS
Sbjct: 241 VYHNQMLQVIHRMFPFGRGLCHAFWAPNFWVFYIMADKLLAFFLRRMGFNVQRPAGSFTS 300

Query: 387 GLVGDSSPFAVLPQITPLVTFIMVLLAISPCLFKAWKNPQPRKVARWITYAYSCGFLFGW 446
           GLVGDSSPFAVLPQITPLVTFIMVLLAISPCLFKAWKNPQPRKVARWITYAYSCGFLFGW
Sbjct: 301 GLVGDSSPFAVLPQITPLVTFIMVLLAISPCLFKAWKNPQPRKVARWITYAYSCGFLFGW 360

Query: 447 HVHEKATLHFLIPLAINAVDSINDARHYFLLSIVSCYSLFPLLFETREYPIKVTLLLLHC 506
           HVHEKATLHFLIPLAINAVDSINDARHYFLLSIVSCYSLFPLLFETREYPIKVTLLLLHC
Sbjct: 361 HVHEKATLHFLIPLAINAVDSINDARHYFLLSIVSCYSLFPLLFETREYPIKVTLLLLHC 420

Query: 507 VLMWFGFSSYFSGETTTNSNKHLQKKNYEDISDKGKLGVGVGVGWLCKSYLLGLVAVEIW 566
           VLMWFGFSSYFSGETTTNSNKHLQKKNYEDISDKGKLGVGVGVGWLCKSYLLGLVAVEIW
Sbjct: 421 VLMWFGFSSYFSGETTTNSNKHLQKKNYEDISDKGKLGVGVGVGWLCKSYLLGLVAVEIW 480

Query: 567 GQFLHTLFFGNKLPFLPLMLVSEALA---TYSVVWMWSTTICSS 601
           GQFLHTLFFGNKLPFLPLMLVS   A    YS +W     I SS
Sbjct: 481 GQFLHTLFFGNKLPFLPLMLVSVYSALGIMYSWIWQLKWIILSS 524

		  

BLAST of Spo11449.1 vs. NCBI nr
Match: gi|731360749|ref|XP_010691987.1| (PREDICTED: probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 795.8 bits (2054), Expect = 6.100e-227
Identity = 393/523 (75.14%), Postives = 441/523 (84.32%), Query Frame = 1

		  

Query: 94  PSKPIKEILWFSLFSTSIKLLLLPSYRSTDFEVHRNWLATTHSLPLSHWYSDTTSQWTLD 153
           PS+P+KE++WFSL STS+KLLL+PSY STDFEVHRNW+ATTHSL L  WYSDTTS WTLD
Sbjct: 17  PSQPMKELIWFSLISTSMKLLLIPSYHSTDFEVHRNWMATTHSLSLPDWYSDTTSPWTLD 76

Query: 154 YPPFFAYFELFLSFIAAQIDPAIVNLHEGLNYKADSVIYFQRITVIVSDLILHYAIYRIT 213
           YPPFFAYFELFLS  AA+IDP IVN  EGLNYKA+SVIYFQRITV+VSDL+LHYAIYRIT
Sbjct: 77  YPPFFAYFELFLSLFAARIDPTIVNHIEGLNYKANSVIYFQRITVMVSDLVLHYAIYRIT 136

Query: 214 RNLDSTKRRLIWILVIWSPALLIVDHMHFQYNGFLLGFLLISISALIEGRDLMGGFFFAV 273
           RNL+S KRRLIW+LVIWSPALLIVDHMHFQYNGFLLG LLISIS L+EG DL+GGFFFAV
Sbjct: 137 RNLESNKRRLIWVLVIWSPALLIVDHMHFQYNGFLLGLLLISISGLMEGNDLVGGFFFAV 196

Query: 274 LLCFKHLFAVAAPVYFVYLLRHYCRGGVQHTPGSHQMMLRALMQNSSACM---------K 333
           LLCFKHLFAVAAPVYFVYLLRHYCRGG     G  ++ +  ++                +
Sbjct: 197 LLCFKHLFAVAAPVYFVYLLRHYCRGG--WIKGFRRLFVLGMVVVLVFAAAFGPFVYHDQ 256

Query: 334 MLQVIHRMFPFGRGLCHAFWAPNFWVFYIMADKLLAFFLRRMGFNVQRPAGSFTSGLVGD 393
           MLQVIHRMFPFGRGLCHA+WAPNFWVFYIMADKL+AF LRR+GF VQRPAGSFTSGLVGD
Sbjct: 257 MLQVIHRMFPFGRGLCHAYWAPNFWVFYIMADKLIAFILRRVGFTVQRPAGSFTSGLVGD 316

Query: 394 SSPFAVLPQITPLVTFIMVLLAISPCLFKAWKNPQPRKVARWITYAYSCGFLFGWHVHEK 453
           SSPF+VLPQITPLVTF+MVLLAISPCLFKAWKNPQP+K+ARWI YAYSCGF+FGWHVHEK
Sbjct: 317 SSPFSVLPQITPLVTFMMVLLAISPCLFKAWKNPQPKKIARWIAYAYSCGFIFGWHVHEK 376

Query: 454 ATLHFLIPLAINAVDSINDARHYFLLSIVSCYSLFPLLFETREYPIKVTLLLLHCVLMWF 513
           ATLHFLIP+A+ A+DSI+DARHYFLLSIV+CYSLFPLLFE +EYPIKVTLLLLHCVLMW+
Sbjct: 377 ATLHFLIPMAVTAIDSISDARHYFLLSIVACYSLFPLLFEVQEYPIKVTLLLLHCVLMWY 436

Query: 514 GFSSYFSGETTTNSNKHLQKKNYEDISDKGKLGVG----VGVGWLCKSYLLGLVAVEIWG 573
           GFSS+FSG TTT S  HLQKKN +  SDK    +     + VGW+CKSYLLG +AVE+WG
Sbjct: 437 GFSSFFSGTTTTTSKNHLQKKNDDLRSDKNLTSISEKGRLDVGWVCKSYLLGFLAVELWG 496

Query: 574 QFLHTLFFGNKLPFLPLMLVSEALA---TYSVVWM--WSTTIC 599
           QFLH +  G++LPFLPL+LVS   A    YS +W   W    C
Sbjct: 497 QFLHPIILGDELPFLPLLLVSVYSAFGIMYSWIWQLKWIVLYC 537

		  

BLAST of Spo11449.1 vs. NCBI nr
Match: gi|356528218|ref|XP_003532702.1| (PREDICTED: probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase [Glycine max])

HSP 1 Score: 669.1 bits (1725), Expect = 8.600e-189
Identity = 331/519 (63.78%), Postives = 392/519 (75.53%), Query Frame = 1

		  

Query: 89  KQKQKPSKPIKEILWFSLFSTSIKLLLLPSYRSTDFEVHRNWLATTHSLPLSHWYSDTTS 148
           K+      P   + WF L +T IK+LL PSYRSTDFEVHRNWLA THSLPLS WY D TS
Sbjct: 9   KEVHHSHSPKTTLWWFFLVATCIKVLLFPSYRSTDFEVHRNWLALTHSLPLSQWYFDETS 68

Query: 149 QWTLDYPPFFAYFELFLSFIAAQIDPAIVNLHEGLNYKADSVIYFQRITVIVSDLILHYA 208
            WTLDYPPFFAYFE FLS  A  IDP IV+L  GLNY ++ V+YFQR+TVI+SDL L Y 
Sbjct: 69  PWTLDYPPFFAYFERFLSIFAHLIDPQIVHLQNGLNYSSNKVVYFQRVTVILSDLSLLYG 128

Query: 209 IYRITRNLDSTKRRLIWILVIWSPALLIVDHMHFQYNGFLLGFLLISISALIEGRDLMGG 268
           +YR+TRNLDS K++LIW LVIWSP L IVDH+HFQYNGFL+G LLIS+S L EGRDL+GG
Sbjct: 129 VYRLTRNLDSRKQQLIWSLVIWSPMLFIVDHVHFQYNGFLIGILLISLSYLEEGRDLLGG 188

Query: 269 FFFAVLLCFKHLFAVAAPVYFVYLLRHYCRGGVQHTPGSHQMMLRALMQNSSACM----- 328
           F FAVLLCFKHLFAVAAP+YFVYLLRHYC GG+    G  ++++   +  +         
Sbjct: 189 FVFAVLLCFKHLFAVAAPIYFVYLLRHYCWGGMVR--GFRRLLIMGGVVTAVFASAFGPF 248

Query: 329 ----KMLQVIHRMFPFGRGLCHAFWAPNFWVFYIMADKLLAFFLRRMGFNVQRPAGSFTS 388
               ++ QVI R+FPFGRGLCHA+WAPNFWVFYIM+DK LAF  R++GFNVQ P GSFT+
Sbjct: 249 FHLGQIQQVIQRLFPFGRGLCHAYWAPNFWVFYIMSDKGLAFIFRKLGFNVQTPTGSFTA 308

Query: 389 GLVGDSSPFAVLPQITPLVTFIMVLLAISPCLFKAWKNPQPRKVARWITYAYSCGFLFGW 448
           GLVGDSSPF+VLPQI P VTFIMVLLA+SPCLFKAWKNPQP+ ++RWI YAY+CGFLFGW
Sbjct: 309 GLVGDSSPFSVLPQIKPSVTFIMVLLALSPCLFKAWKNPQPQMISRWIAYAYTCGFLFGW 368

Query: 449 HVHEKATLHFLIPLAINAVDSINDARHYFLLSIVSCYSLFPLLFETREYPIKVTLLLLHC 508
           HVHEKA+LHF+IPLAI A  ++ DARHYFLLSIVSCYS+FPLLFE REYPIKV LLLLH 
Sbjct: 369 HVHEKASLHFVIPLAIVAAQTLEDARHYFLLSIVSCYSIFPLLFEAREYPIKVLLLLLHS 428

Query: 509 VLMWFGFSS-YFSGETTTNSNKHLQKKNYEDISDKGKLGV----GVGVGWLCKSYLLGLV 568
           +LMW GFS+ ++ G   T       KK  +    +G  G     G  +GW+ + YL+GLV
Sbjct: 429 ILMWSGFSAQFYDGAEATRVPTANSKKKADQFGSEGNSGATVKKGFAIGWIERIYLIGLV 488

Query: 569 AVEIWGQFLHTLFFGNKLPFLPLMLVSEALATYSVVWMW 594
            VEIWGQ LH L FG+K  F PLML+S     + +++ W
Sbjct: 489 VVEIWGQILHPLLFGDKFAFAPLMLIS-IYCAFGIMYSW 524

		  

BLAST of Spo11449.1 vs. NCBI nr
Match: gi|734350491|gb|KHN12438.1| (Putative dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase [Glycine soja])

HSP 1 Score: 669.1 bits (1725), Expect = 8.600e-189
Identity = 331/519 (63.78%), Postives = 392/519 (75.53%), Query Frame = 1

		  

Query: 89  KQKQKPSKPIKEILWFSLFSTSIKLLLLPSYRSTDFEVHRNWLATTHSLPLSHWYSDTTS 148
           K+      P   + WF L +T IK+LL PSYRSTDFEVHRNWLA THSLPLS WY D TS
Sbjct: 9   KEVHHSHSPKTTLWWFFLVATCIKVLLFPSYRSTDFEVHRNWLALTHSLPLSQWYFDETS 68

Query: 149 QWTLDYPPFFAYFELFLSFIAAQIDPAIVNLHEGLNYKADSVIYFQRITVIVSDLILHYA 208
            WTLDYPPFFAYFE FLS  A  IDP IV+L  GLNY ++ V+YFQR+TVI+SDL L Y 
Sbjct: 69  PWTLDYPPFFAYFERFLSIFAHLIDPQIVHLQNGLNYSSNKVVYFQRVTVILSDLSLLYG 128

Query: 209 IYRITRNLDSTKRRLIWILVIWSPALLIVDHMHFQYNGFLLGFLLISISALIEGRDLMGG 268
           +YR+TRNLDS K++LIW LVIWSP L IVDH+HFQYNGFL+G LLIS+S L EGRDL+GG
Sbjct: 129 VYRLTRNLDSRKQQLIWSLVIWSPMLFIVDHVHFQYNGFLIGILLISLSYLEEGRDLLGG 188

Query: 269 FFFAVLLCFKHLFAVAAPVYFVYLLRHYCRGGVQHTPGSHQMMLRALMQNSSACM----- 328
           F FAVLLCFKHLFAVAAP+YFVYLLRHYC GG+    G  ++++   +  +         
Sbjct: 189 FVFAVLLCFKHLFAVAAPIYFVYLLRHYCWGGMVR--GFRRLLIMGGVVTAVFASAFGPF 248

Query: 329 ----KMLQVIHRMFPFGRGLCHAFWAPNFWVFYIMADKLLAFFLRRMGFNVQRPAGSFTS 388
               ++ QVI R+FPFGRGLCHA+WAPNFWVFYIM+DK LAF  R++GFNVQ P GSFT+
Sbjct: 249 FHLGQIQQVIQRLFPFGRGLCHAYWAPNFWVFYIMSDKGLAFIFRKLGFNVQTPTGSFTA 308

Query: 389 GLVGDSSPFAVLPQITPLVTFIMVLLAISPCLFKAWKNPQPRKVARWITYAYSCGFLFGW 448
           GLVGDSSPF+VLPQI P VTFIMVLLA+SPCLFKAWKNPQP+ ++RWI YAY+CGFLFGW
Sbjct: 309 GLVGDSSPFSVLPQIKPSVTFIMVLLALSPCLFKAWKNPQPQMISRWIAYAYTCGFLFGW 368

Query: 449 HVHEKATLHFLIPLAINAVDSINDARHYFLLSIVSCYSLFPLLFETREYPIKVTLLLLHC 508
           HVHEKA+LHF+IPLAI A  ++ DARHYFLLSIVSCYS+FPLLFE REYPIKV LLLLH 
Sbjct: 369 HVHEKASLHFVIPLAIVAAQTLEDARHYFLLSIVSCYSIFPLLFEAREYPIKVLLLLLHS 428

Query: 509 VLMWFGFSS-YFSGETTTNSNKHLQKKNYEDISDKGKLGV----GVGVGWLCKSYLLGLV 568
           +LMW GFS+ ++ G   T       KK  +    +G  G     G  +GW+ + YL+GLV
Sbjct: 429 ILMWSGFSAQFYDGAEATRVPTANSKKKADQFGSEGNSGATVKKGFAIGWIERIYLIGLV 488

Query: 569 AVEIWGQFLHTLFFGNKLPFLPLMLVSEALATYSVVWMW 594
            VEIWGQ LH L FG+K  F PLML+S     + +++ W
Sbjct: 489 VVEIWGQILHPLLFGDKFAFAPLMLIS-IYCAFGIMYSW 524

		  

BLAST of Spo11449.1 vs. NCBI nr
Match: gi|565376297|ref|XP_006354643.1| (PREDICTED: probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase [Solanum tuberosum])

HSP 1 Score: 667.5 bits (1721), Expect = 2.500e-188
Identity = 335/511 (65.56%), Postives = 397/511 (77.69%), Query Frame = 1

		  

Query: 91  KQKPSKPIKEILWFSLFSTSIKLLLLPSYRSTDFEVHRNWLATTHSLPLSHWYSDTTSQW 150
           K KP++PI EILW SL +T IK LL+P+Y STDFEVHRNWLA THSLPLS WYSD TS W
Sbjct: 44  KLKPNRPIIEILWVSLVATCIKFLLIPAYHSTDFEVHRNWLAITHSLPLSQWYSDETSPW 103

Query: 151 TLDYPPFFAYFELFLSFIAAQIDPAIVNLHEGLNYKADSVIYFQRITVIVSDLILHYAIY 210
           TLDYPPFFA+FE FLSF A+ +DP +VNL  GLNYK+   I FQR+TVIVSD +L + IY
Sbjct: 104 TLDYPPFFAHFEHFLSFFASLVDPIMVNLQHGLNYKSQPTILFQRLTVIVSDFVLVFGIY 163

Query: 211 RITRNLDSTKRRLIWILVIWSPALLIVDHMHFQYNGFLLGFLLISISALIEGRDLMGGFF 270
           R+TRNL   +R LIW+LVI+SP L IVDH+HFQYNGFLLG LLIS+SAL EG+DL+GGF 
Sbjct: 164 RLTRNLGYKERVLIWVLVIFSPGLTIVDHLHFQYNGFLLGILLISLSALEEGKDLLGGFV 223

Query: 271 FAVLLCFKHLFAVAAPVYFVYLLRHYCRGGVQHTPGSHQMMLRALMQNSSACM------- 330
           FAVLLCFKHLFAVAAPVYFVYLLRHYCRGG+    G   +M  A++   +A         
Sbjct: 224 FAVLLCFKHLFAVAAPVYFVYLLRHYCRGGLIRGFGRLVIMGSAVVAVFAAAYGPFLYHG 283

Query: 331 KMLQVIHRMFPFGRGLCHAFWAPNFWVFYIMADKLLAFFLRRMGFNVQRPAGSFTSGLVG 390
           ++ QV HRMFPFGRGLCHA+WAPNFWVFYI+ DK+LAF L +MGF+VQ P  SFT GLVG
Sbjct: 284 QIQQVFHRMFPFGRGLCHAYWAPNFWVFYIILDKVLAFSLVKMGFSVQTPTASFTGGLVG 343

Query: 391 DSSPFAVLPQITPLVTFIMVLLAISPCLFKAWKNPQPRKVARWITYAYSCGFLFGWHVHE 450
           DSSPFAVLP++TP+VTF +VLLAI+PCL KAWK+PQPR + RWI YAY+CGF+FGWHVHE
Sbjct: 344 DSSPFAVLPRVTPMVTFSIVLLAIAPCLIKAWKDPQPRMITRWIAYAYTCGFMFGWHVHE 403

Query: 451 KATLHFLIPLAINAVDSINDARHYFLLSIVSCYSLFPLLFETREYPIKVTLLLLHCVLMW 510
           KA+LHF+IPLAI ++ S+ DA+HYF LS+VSCYSLFPLLFE +EYPIKV LLLLH  LMW
Sbjct: 404 KASLHFVIPLAIISLKSVEDAKHYFYLSVVSCYSLFPLLFEAQEYPIKVLLLLLHATLMW 463

Query: 511 FGFSSYFSGETTTNSNKHLQKKNYEDISDKGKLGVGVGVGWLCKSYLLGLVAVEIWGQFL 570
            GFSS+F+  TT       ++  Y    DK +      VGW  K YLLGLVAVEI+GQF+
Sbjct: 464 IGFSSHFT--TTNRKAAESEQTKY----DKTRF----IVGWFGKLYLLGLVAVEIYGQFV 523

Query: 571 HTLFFGNKLPFLPLMLVSEALA---TYSVVW 592
           H + F  +LPFLPLM++S   A    YS +W
Sbjct: 524 HPILFAERLPFLPLMMISIYCAFGMMYSWIW 544

		  

BLAST of Spo11449.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RYY2_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_018370 PE=4 SV=1)

HSP 1 Score: 954.5 bits (2466), Expect = 7.100e-275
Identity = 482/524 (91.98%), Postives = 488/524 (93.13%), Query Frame = 1

		  

Query: 87  MEKQKQKPSKPIKEILWFSLFSTSIKLLLLPSYRSTDFEVHRNWLATTHSLPLSHWYSDT 146
           MEKQKQKPSKPIKEILWFSLFSTSIKLLLLPSYRSTDFEVHRNWLATTHSLPLSHWYSDT
Sbjct: 1   MEKQKQKPSKPIKEILWFSLFSTSIKLLLLPSYRSTDFEVHRNWLATTHSLPLSHWYSDT 60

Query: 147 TSQWTLDYPPFFAYFELFLSFIAAQIDPAIVNLHEGLNYKADSVIYFQRITVIVSDLILH 206
           TSQWTLDYPPFFAYFELFLSFIAAQIDPAIVNLHEGLNYKADSVIYFQRITVIVSDLILH
Sbjct: 61  TSQWTLDYPPFFAYFELFLSFIAAQIDPAIVNLHEGLNYKADSVIYFQRITVIVSDLILH 120

Query: 207 YAIYRITRNLDSTKRRLIWILVIWSPALLIVDHMHFQYNGFLLGFLLISISALIEGRDLM 266
           YAIYRITRNLDSTKRRLIWILVIWSPALLIVDHMHFQYNGFLLGFLLISISALIEGRDLM
Sbjct: 121 YAIYRITRNLDSTKRRLIWILVIWSPALLIVDHMHFQYNGFLLGFLLISISALIEGRDLM 180

Query: 267 GGFFFAVLLCFKHLFAVAAPVYFVYLLRHYCRGGVQHTPGSHQMMLRALMQNSSACM--- 326
           GGFFFAVLLCFKHLFAVAAPVYFVYLLRHYCRGG+    G   +M  A++    A     
Sbjct: 181 GGFFFAVLLCFKHLFAVAAPVYFVYLLRHYCRGGLFKGFGRLLLMGLAVVVVFVAAFWPF 240

Query: 327 ----KMLQVIHRMFPFGRGLCHAFWAPNFWVFYIMADKLLAFFLRRMGFNVQRPAGSFTS 386
               +MLQVIHRMFPFGRGLCHAFWAPNFWVFYIMADKLLAFFLRRMGFNVQRPAGSFTS
Sbjct: 241 VYHNQMLQVIHRMFPFGRGLCHAFWAPNFWVFYIMADKLLAFFLRRMGFNVQRPAGSFTS 300

Query: 387 GLVGDSSPFAVLPQITPLVTFIMVLLAISPCLFKAWKNPQPRKVARWITYAYSCGFLFGW 446
           GLVGDSSPFAVLPQITPLVTFIMVLLAISPCLFKAWKNPQPRKVARWITYAYSCGFLFGW
Sbjct: 301 GLVGDSSPFAVLPQITPLVTFIMVLLAISPCLFKAWKNPQPRKVARWITYAYSCGFLFGW 360

Query: 447 HVHEKATLHFLIPLAINAVDSINDARHYFLLSIVSCYSLFPLLFETREYPIKVTLLLLHC 506
           HVHEKATLHFLIPLAINAVDSINDARHYFLLSIVSCYSLFPLLFETREYPIKVTLLLLHC
Sbjct: 361 HVHEKATLHFLIPLAINAVDSINDARHYFLLSIVSCYSLFPLLFETREYPIKVTLLLLHC 420

Query: 507 VLMWFGFSSYFSGETTTNSNKHLQKKNYEDISDKGKLGVGVGVGWLCKSYLLGLVAVEIW 566
           VLMWFGFSSYFSGETTTNSNKHLQKKNYEDISDKGKLGVGVGVGWLCKSYLLGLVAVEIW
Sbjct: 421 VLMWFGFSSYFSGETTTNSNKHLQKKNYEDISDKGKLGVGVGVGWLCKSYLLGLVAVEIW 480

Query: 567 GQFLHTLFFGNKLPFLPLMLVSEALA---TYSVVWMWSTTICSS 601
           GQFLHTLFFGNKLPFLPLMLVS   A    YS +W     I SS
Sbjct: 481 GQFLHTLFFGNKLPFLPLMLVSVYSALGIMYSWIWQLKWIILSS 524

		  

BLAST of Spo11449.1 vs. UniProtKB/TrEMBL
Match: A0A0J8E9W6_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_1g017260 PE=4 SV=1)

HSP 1 Score: 795.8 bits (2054), Expect = 4.200e-227
Identity = 393/523 (75.14%), Postives = 441/523 (84.32%), Query Frame = 1

		  

Query: 94  PSKPIKEILWFSLFSTSIKLLLLPSYRSTDFEVHRNWLATTHSLPLSHWYSDTTSQWTLD 153
           PS+P+KE++WFSL STS+KLLL+PSY STDFEVHRNW+ATTHSL L  WYSDTTS WTLD
Sbjct: 17  PSQPMKELIWFSLISTSMKLLLIPSYHSTDFEVHRNWMATTHSLSLPDWYSDTTSPWTLD 76

Query: 154 YPPFFAYFELFLSFIAAQIDPAIVNLHEGLNYKADSVIYFQRITVIVSDLILHYAIYRIT 213
           YPPFFAYFELFLS  AA+IDP IVN  EGLNYKA+SVIYFQRITV+VSDL+LHYAIYRIT
Sbjct: 77  YPPFFAYFELFLSLFAARIDPTIVNHIEGLNYKANSVIYFQRITVMVSDLVLHYAIYRIT 136

Query: 214 RNLDSTKRRLIWILVIWSPALLIVDHMHFQYNGFLLGFLLISISALIEGRDLMGGFFFAV 273
           RNL+S KRRLIW+LVIWSPALLIVDHMHFQYNGFLLG LLISIS L+EG DL+GGFFFAV
Sbjct: 137 RNLESNKRRLIWVLVIWSPALLIVDHMHFQYNGFLLGLLLISISGLMEGNDLVGGFFFAV 196

Query: 274 LLCFKHLFAVAAPVYFVYLLRHYCRGGVQHTPGSHQMMLRALMQNSSACM---------K 333
           LLCFKHLFAVAAPVYFVYLLRHYCRGG     G  ++ +  ++                +
Sbjct: 197 LLCFKHLFAVAAPVYFVYLLRHYCRGG--WIKGFRRLFVLGMVVVLVFAAAFGPFVYHDQ 256

Query: 334 MLQVIHRMFPFGRGLCHAFWAPNFWVFYIMADKLLAFFLRRMGFNVQRPAGSFTSGLVGD 393
           MLQVIHRMFPFGRGLCHA+WAPNFWVFYIMADKL+AF LRR+GF VQRPAGSFTSGLVGD
Sbjct: 257 MLQVIHRMFPFGRGLCHAYWAPNFWVFYIMADKLIAFILRRVGFTVQRPAGSFTSGLVGD 316

Query: 394 SSPFAVLPQITPLVTFIMVLLAISPCLFKAWKNPQPRKVARWITYAYSCGFLFGWHVHEK 453
           SSPF+VLPQITPLVTF+MVLLAISPCLFKAWKNPQP+K+ARWI YAYSCGF+FGWHVHEK
Sbjct: 317 SSPFSVLPQITPLVTFMMVLLAISPCLFKAWKNPQPKKIARWIAYAYSCGFIFGWHVHEK 376

Query: 454 ATLHFLIPLAINAVDSINDARHYFLLSIVSCYSLFPLLFETREYPIKVTLLLLHCVLMWF 513
           ATLHFLIP+A+ A+DSI+DARHYFLLSIV+CYSLFPLLFE +EYPIKVTLLLLHCVLMW+
Sbjct: 377 ATLHFLIPMAVTAIDSISDARHYFLLSIVACYSLFPLLFEVQEYPIKVTLLLLHCVLMWY 436

Query: 514 GFSSYFSGETTTNSNKHLQKKNYEDISDKGKLGVG----VGVGWLCKSYLLGLVAVEIWG 573
           GFSS+FSG TTT S  HLQKKN +  SDK    +     + VGW+CKSYLLG +AVE+WG
Sbjct: 437 GFSSFFSGTTTTTSKNHLQKKNDDLRSDKNLTSISEKGRLDVGWVCKSYLLGFLAVELWG 496

Query: 574 QFLHTLFFGNKLPFLPLMLVSEALA---TYSVVWM--WSTTIC 599
           QFLH +  G++LPFLPL+LVS   A    YS +W   W    C
Sbjct: 497 QFLHPIILGDELPFLPLLLVSVYSAFGIMYSWIWQLKWIVLYC 537

		  

BLAST of Spo11449.1 vs. UniProtKB/TrEMBL
Match: A0A0B2PXF6_GLYSO (Putative dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Glycine soja GN=glysoja_018568 PE=4 SV=1)

HSP 1 Score: 669.1 bits (1725), Expect = 6.000e-189
Identity = 331/519 (63.78%), Postives = 392/519 (75.53%), Query Frame = 1

		  

Query: 89  KQKQKPSKPIKEILWFSLFSTSIKLLLLPSYRSTDFEVHRNWLATTHSLPLSHWYSDTTS 148
           K+      P   + WF L +T IK+LL PSYRSTDFEVHRNWLA THSLPLS WY D TS
Sbjct: 9   KEVHHSHSPKTTLWWFFLVATCIKVLLFPSYRSTDFEVHRNWLALTHSLPLSQWYFDETS 68

Query: 149 QWTLDYPPFFAYFELFLSFIAAQIDPAIVNLHEGLNYKADSVIYFQRITVIVSDLILHYA 208
            WTLDYPPFFAYFE FLS  A  IDP IV+L  GLNY ++ V+YFQR+TVI+SDL L Y 
Sbjct: 69  PWTLDYPPFFAYFERFLSIFAHLIDPQIVHLQNGLNYSSNKVVYFQRVTVILSDLSLLYG 128

Query: 209 IYRITRNLDSTKRRLIWILVIWSPALLIVDHMHFQYNGFLLGFLLISISALIEGRDLMGG 268
           +YR+TRNLDS K++LIW LVIWSP L IVDH+HFQYNGFL+G LLIS+S L EGRDL+GG
Sbjct: 129 VYRLTRNLDSRKQQLIWSLVIWSPMLFIVDHVHFQYNGFLIGILLISLSYLEEGRDLLGG 188

Query: 269 FFFAVLLCFKHLFAVAAPVYFVYLLRHYCRGGVQHTPGSHQMMLRALMQNSSACM----- 328
           F FAVLLCFKHLFAVAAP+YFVYLLRHYC GG+    G  ++++   +  +         
Sbjct: 189 FVFAVLLCFKHLFAVAAPIYFVYLLRHYCWGGMVR--GFRRLLIMGGVVTAVFASAFGPF 248

Query: 329 ----KMLQVIHRMFPFGRGLCHAFWAPNFWVFYIMADKLLAFFLRRMGFNVQRPAGSFTS 388
               ++ QVI R+FPFGRGLCHA+WAPNFWVFYIM+DK LAF  R++GFNVQ P GSFT+
Sbjct: 249 FHLGQIQQVIQRLFPFGRGLCHAYWAPNFWVFYIMSDKGLAFIFRKLGFNVQTPTGSFTA 308

Query: 389 GLVGDSSPFAVLPQITPLVTFIMVLLAISPCLFKAWKNPQPRKVARWITYAYSCGFLFGW 448
           GLVGDSSPF+VLPQI P VTFIMVLLA+SPCLFKAWKNPQP+ ++RWI YAY+CGFLFGW
Sbjct: 309 GLVGDSSPFSVLPQIKPSVTFIMVLLALSPCLFKAWKNPQPQMISRWIAYAYTCGFLFGW 368

Query: 449 HVHEKATLHFLIPLAINAVDSINDARHYFLLSIVSCYSLFPLLFETREYPIKVTLLLLHC 508
           HVHEKA+LHF+IPLAI A  ++ DARHYFLLSIVSCYS+FPLLFE REYPIKV LLLLH 
Sbjct: 369 HVHEKASLHFVIPLAIVAAQTLEDARHYFLLSIVSCYSIFPLLFEAREYPIKVLLLLLHS 428

Query: 509 VLMWFGFSS-YFSGETTTNSNKHLQKKNYEDISDKGKLGV----GVGVGWLCKSYLLGLV 568
           +LMW GFS+ ++ G   T       KK  +    +G  G     G  +GW+ + YL+GLV
Sbjct: 429 ILMWSGFSAQFYDGAEATRVPTANSKKKADQFGSEGNSGATVKKGFAIGWIERIYLIGLV 488

Query: 569 AVEIWGQFLHTLFFGNKLPFLPLMLVSEALATYSVVWMW 594
            VEIWGQ LH L FG+K  F PLML+S     + +++ W
Sbjct: 489 VVEIWGQILHPLLFGDKFAFAPLMLIS-IYCAFGIMYSW 524

		  

BLAST of Spo11449.1 vs. UniProtKB/TrEMBL
Match: I1KRW2_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_08G098700 PE=4 SV=1)

HSP 1 Score: 669.1 bits (1725), Expect = 6.000e-189
Identity = 331/519 (63.78%), Postives = 392/519 (75.53%), Query Frame = 1

		  

Query: 89  KQKQKPSKPIKEILWFSLFSTSIKLLLLPSYRSTDFEVHRNWLATTHSLPLSHWYSDTTS 148
           K+      P   + WF L +T IK+LL PSYRSTDFEVHRNWLA THSLPLS WY D TS
Sbjct: 9   KEVHHSHSPKTTLWWFFLVATCIKVLLFPSYRSTDFEVHRNWLALTHSLPLSQWYFDETS 68

Query: 149 QWTLDYPPFFAYFELFLSFIAAQIDPAIVNLHEGLNYKADSVIYFQRITVIVSDLILHYA 208
            WTLDYPPFFAYFE FLS  A  IDP IV+L  GLNY ++ V+YFQR+TVI+SDL L Y 
Sbjct: 69  PWTLDYPPFFAYFERFLSIFAHLIDPQIVHLQNGLNYSSNKVVYFQRVTVILSDLSLLYG 128

Query: 209 IYRITRNLDSTKRRLIWILVIWSPALLIVDHMHFQYNGFLLGFLLISISALIEGRDLMGG 268
           +YR+TRNLDS K++LIW LVIWSP L IVDH+HFQYNGFL+G LLIS+S L EGRDL+GG
Sbjct: 129 VYRLTRNLDSRKQQLIWSLVIWSPMLFIVDHVHFQYNGFLIGILLISLSYLEEGRDLLGG 188

Query: 269 FFFAVLLCFKHLFAVAAPVYFVYLLRHYCRGGVQHTPGSHQMMLRALMQNSSACM----- 328
           F FAVLLCFKHLFAVAAP+YFVYLLRHYC GG+    G  ++++   +  +         
Sbjct: 189 FVFAVLLCFKHLFAVAAPIYFVYLLRHYCWGGMVR--GFRRLLIMGGVVTAVFASAFGPF 248

Query: 329 ----KMLQVIHRMFPFGRGLCHAFWAPNFWVFYIMADKLLAFFLRRMGFNVQRPAGSFTS 388
               ++ QVI R+FPFGRGLCHA+WAPNFWVFYIM+DK LAF  R++GFNVQ P GSFT+
Sbjct: 249 FHLGQIQQVIQRLFPFGRGLCHAYWAPNFWVFYIMSDKGLAFIFRKLGFNVQTPTGSFTA 308

Query: 389 GLVGDSSPFAVLPQITPLVTFIMVLLAISPCLFKAWKNPQPRKVARWITYAYSCGFLFGW 448
           GLVGDSSPF+VLPQI P VTFIMVLLA+SPCLFKAWKNPQP+ ++RWI YAY+CGFLFGW
Sbjct: 309 GLVGDSSPFSVLPQIKPSVTFIMVLLALSPCLFKAWKNPQPQMISRWIAYAYTCGFLFGW 368

Query: 449 HVHEKATLHFLIPLAINAVDSINDARHYFLLSIVSCYSLFPLLFETREYPIKVTLLLLHC 508
           HVHEKA+LHF+IPLAI A  ++ DARHYFLLSIVSCYS+FPLLFE REYPIKV LLLLH 
Sbjct: 369 HVHEKASLHFVIPLAIVAAQTLEDARHYFLLSIVSCYSIFPLLFEAREYPIKVLLLLLHS 428

Query: 509 VLMWFGFSS-YFSGETTTNSNKHLQKKNYEDISDKGKLGV----GVGVGWLCKSYLLGLV 568
           +LMW GFS+ ++ G   T       KK  +    +G  G     G  +GW+ + YL+GLV
Sbjct: 429 ILMWSGFSAQFYDGAEATRVPTANSKKKADQFGSEGNSGATVKKGFAIGWIERIYLIGLV 488

Query: 569 AVEIWGQFLHTLFFGNKLPFLPLMLVSEALATYSVVWMW 594
            VEIWGQ LH L FG+K  F PLML+S     + +++ W
Sbjct: 489 VVEIWGQILHPLLFGDKFAFAPLMLIS-IYCAFGIMYSW 524

		  

BLAST of Spo11449.1 vs. UniProtKB/TrEMBL
Match: M0ZN96_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG402001720 PE=4 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 1.700e-188
Identity = 335/511 (65.56%), Postives = 397/511 (77.69%), Query Frame = 1

		  

Query: 91  KQKPSKPIKEILWFSLFSTSIKLLLLPSYRSTDFEVHRNWLATTHSLPLSHWYSDTTSQW 150
           K KP++PI EILW SL +T IK LL+P+Y STDFEVHRNWLA THSLPLS WYSD TS W
Sbjct: 44  KLKPNRPIIEILWVSLVATCIKFLLIPAYHSTDFEVHRNWLAITHSLPLSQWYSDETSPW 103

Query: 151 TLDYPPFFAYFELFLSFIAAQIDPAIVNLHEGLNYKADSVIYFQRITVIVSDLILHYAIY 210
           TLDYPPFFA+FE FLSF A+ +DP +VNL  GLNYK+   I FQR+TVIVSD +L + IY
Sbjct: 104 TLDYPPFFAHFEHFLSFFASLVDPIMVNLQHGLNYKSQPTILFQRLTVIVSDFVLVFGIY 163

Query: 211 RITRNLDSTKRRLIWILVIWSPALLIVDHMHFQYNGFLLGFLLISISALIEGRDLMGGFF 270
           R+TRNL   +R LIW+LVI+SP L IVDH+HFQYNGFLLG LLIS+SAL EG+DL+GGF 
Sbjct: 164 RLTRNLGYKERVLIWVLVIFSPGLTIVDHLHFQYNGFLLGILLISLSALEEGKDLLGGFV 223

Query: 271 FAVLLCFKHLFAVAAPVYFVYLLRHYCRGGVQHTPGSHQMMLRALMQNSSACM------- 330
           FAVLLCFKHLFAVAAPVYFVYLLRHYCRGG+    G   +M  A++   +A         
Sbjct: 224 FAVLLCFKHLFAVAAPVYFVYLLRHYCRGGLIRGFGRLVIMGSAVVAVFAAAYGPFLYHG 283

Query: 331 KMLQVIHRMFPFGRGLCHAFWAPNFWVFYIMADKLLAFFLRRMGFNVQRPAGSFTSGLVG 390
           ++ QV HRMFPFGRGLCHA+WAPNFWVFYI+ DK+LAF L +MGF+VQ P  SFT GLVG
Sbjct: 284 QIQQVFHRMFPFGRGLCHAYWAPNFWVFYIILDKVLAFSLVKMGFSVQTPTASFTGGLVG 343

Query: 391 DSSPFAVLPQITPLVTFIMVLLAISPCLFKAWKNPQPRKVARWITYAYSCGFLFGWHVHE 450
           DSSPFAVLP++TP+VTF +VLLAI+PCL KAWK+PQPR + RWI YAY+CGF+FGWHVHE
Sbjct: 344 DSSPFAVLPRVTPMVTFSIVLLAIAPCLIKAWKDPQPRMITRWIAYAYTCGFMFGWHVHE 403

Query: 451 KATLHFLIPLAINAVDSINDARHYFLLSIVSCYSLFPLLFETREYPIKVTLLLLHCVLMW 510
           KA+LHF+IPLAI ++ S+ DA+HYF LS+VSCYSLFPLLFE +EYPIKV LLLLH  LMW
Sbjct: 404 KASLHFVIPLAIISLKSVEDAKHYFYLSVVSCYSLFPLLFEAQEYPIKVLLLLLHATLMW 463

Query: 511 FGFSSYFSGETTTNSNKHLQKKNYEDISDKGKLGVGVGVGWLCKSYLLGLVAVEIWGQFL 570
            GFSS+F+  TT       ++  Y    DK +      VGW  K YLLGLVAVEI+GQF+
Sbjct: 464 IGFSSHFT--TTNRKAAESEQTKY----DKTRF----IVGWFGKLYLLGLVAVEIYGQFV 523

Query: 571 HTLFFGNKLPFLPLMLVSEALA---TYSVVW 592
           H + F  +LPFLPLM++S   A    YS +W
Sbjct: 524 HPILFAERLPFLPLMMISIYCAFGMMYSWIW 544

		  

BLAST of Spo11449.1 vs. ExPASy Swiss-Prot
Match: ALG8_ARATH (Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Arabidopsis thaliana GN=At2g44660 PE=2 SV=3)

HSP 1 Score: 627.1 bits (1616), Expect = 2.300e-178
Identity = 321/501 (64.07%), Postives = 379/501 (75.65%), Query Frame = 1

		  

Query: 101 ILWFSLFSTSIKLLLLPSYRSTDFEVHRNWLATTHSLPLSHWYSDTTSQWTLDYPPFFAY 160
           +LWF   +T++KLLL+PS RSTDFEVHRNWLA T+SLPL+ WY D TSQWTLDYPPFFAY
Sbjct: 12  LLWFFAVATAVKLLLIPSSRSTDFEVHRNWLAITNSLPLTKWYFDETSQWTLDYPPFFAY 71

Query: 161 FELFLSFIAAQIDPAIVNLHEGLNYKADSVIYFQRITVIVSDLILHYAIYRITRNLDSTK 220
           FE FLS  A  +DP IV+L  GL+Y A+SVIYFQRI+VIVSDL L Y +YR+TR L+  K
Sbjct: 72  FERFLSIFARLVDPRIVDLQSGLDYNAESVIYFQRISVIVSDLCLLYGVYRLTRKLEPLK 131

Query: 221 RRLIWILVIWSPALLIVDHMHFQYNGFLLGFLLISISALIEGRDLMGGFFFAVLLCFKHL 280
           R LI  LVIWSP LLIVDH+HFQYNGFLLG+LL+SIS L EGRDL+GGF FAVLLCFKHL
Sbjct: 132 RNLICALVIWSPGLLIVDHIHFQYNGFLLGWLLLSISFLQEGRDLLGGFLFAVLLCFKHL 191

Query: 281 FAVAAPVYFVYLLRHYCRGGVQHTPGSHQMMLRALMQNSSACM--------KMLQVIHRM 340
           FAV APVYFVYLLRHYC  G+  T     + + A++    A          ++ QVI RM
Sbjct: 192 FAVTAPVYFVYLLRHYCWSGLV-TGFRRLVTIGAVVVAVFAAAYGPFIYHGQIQQVISRM 251

Query: 341 FPFGRGLCHAFWAPNFWVFYIMADKLLAFFLRRMGFNVQRPAGSFTSGLVGDSSPFAVLP 400
           FPFGRGLCHA+WAPNFWVFYI+ DK LA  LR++G+ +Q P+ SFT GLVGDSSPFAVLP
Sbjct: 252 FPFGRGLCHAYWAPNFWVFYIILDKGLAVLLRKLGYEIQIPSASFTGGLVGDSSPFAVLP 311

Query: 401 QITPLVTFIMVLLAISPCLFKAWKNPQPRKVARWITYAYSCGFLFGWHVHEKATLHFLIP 460
           QITPL TF MVLLAISPCL KAWK P    VARW+ YAY+CGFLFGWHVHEKA+LHF IP
Sbjct: 312 QITPLTTFAMVLLAISPCLIKAWKKPHSGLVARWVAYAYTCGFLFGWHVHEKASLHFTIP 371

Query: 461 LAINAVDSINDARHYFLLSIVSCYSLFPLLFETREYPIKVTLLLLHCVLMWFGFSSYFSG 520
           LAI AV S+ DA+HYFL+SIVSCYSL+PLL+E +EYPIKV LLLLH V+MW GF++ ++ 
Sbjct: 372 LAIVAVQSLEDAKHYFLVSIVSCYSLYPLLYEPQEYPIKVLLLLLHSVVMWLGFAAQYT- 431

Query: 521 ETTTNSNKHLQKKNYEDISDKGKLGVGVGVGWLCKSYLLGLVAVEIWGQFLHTLFFGNKL 580
                 +   QKK   +I  K ++G         KSYL+GL+ VEI  QFLH  F G+KL
Sbjct: 432 ------DYKAQKKETSEIKSKFRIGC------FEKSYLMGLIIVEIVSQFLHPYFLGDKL 491

Query: 581 PFLPLMLVSEALATYSVVWMW 594
           PFLPLML+S    T  +++ W
Sbjct: 492 PFLPLMLIS-TYCTVGIMYSW 497

		  

BLAST of Spo11449.1 vs. ExPASy Swiss-Prot
Match: ALG8_HUMAN (Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Homo sapiens GN=ALG8 PE=1 SV=2)

HSP 1 Score: 345.5 bits (885), Expect = 1.400e-93
Identity = 217/540 (40.19%), Postives = 296/540 (54.81%), Query Frame = 1

		  

Query: 103 WFSLFS---TSIKLLLLPSYRSTDFEVHRNWLATTHSLPLSHWYSDTTSQWTLDYPPFFA 162
           WFS  +   T +K LL+P+Y STDFEVHRNWLA THSLP+S WY + TS+WTLDYPPFFA
Sbjct: 13  WFSALALGVTLLKCLLIPTYHSTDFEVHRNWLAITHSLPISQWYYEATSEWTLDYPPFFA 72

Query: 163 YFELFLSFIAAQIDPAIVNLHEGLNYKADSVIYFQRITVIVSDLILHYAIYRITRNLDST 222
           +FE  LS +A   D  ++N+H  LNY +   + FQR +VI  D++  YA+    + +D  
Sbjct: 73  WFEYILSHVAKYFDQEMLNVH-NLNYSSSRTLLFQRFSVIFMDVLFVYAVRECCKCIDGK 132

Query: 223 K---------RRLIWILVIWSPALLIVDHMHFQYNGFLLGFLLISISALIEGRDLMGGFF 282
           K         + ++ +L++W+  LLIVDH+HFQYNGFL G +L+SI+ L + R + G F 
Sbjct: 133 KVGKELTEKPKFILSVLLLWNFGLLIVDHIHFQYNGFLFGLMLLSIARLFQKRHMEGAFL 192

Query: 283 FAVLLCFKHLFAVAAPVYFVYLLRHYC------RGGVQH---------TPGSHQMMLRAL 342
           FAVLL FKH++   AP Y VYLLR YC       G ++          + G    ++ AL
Sbjct: 193 FAVLLHFKHIYLYVAPAYGVYLLRSYCFTANKPDGSIRWKSFSFVRVISLGLVVFLVSAL 252

Query: 343 MQNSSACMKML-QVIHRMFPFGRGLCHAFWAPNFWVFYIMADKLLAFFLRRMGF----NV 402
                  +  L QV  R+FPF RGLCHA+WAPNFW  Y   DK+L+    ++ F    N+
Sbjct: 253 SLGPFLALNQLPQVFSRLFPFKRGLCHAYWAPNFWALYNALDKVLSVIGLKLKFLDPNNI 312

Query: 403 QRPAGSFTSGLVGDSSPFAVLPQITPLVTFIMVLLAISPCLFKAWKNPQ-PRKVARWITY 462
             P  S TSGLV       VLP +TPL T I  L+AI P +F  W  PQ PR   R +T 
Sbjct: 313 --PKASMTSGLV-QQFQHTVLPSVTPLATLICTLIAILPSIFCLWFKPQGPRGFLRCLTL 372

Query: 463 AYSCGFLFGWHVHEKATLHFLIPLAINAVDSINDARHYFLLSIVSCYSLFPLLFETREYP 522
                F+FGWHVHEKA L  ++P+++ +V    DA  + +L+    YSLFPLLF   E P
Sbjct: 373 CALSSFMFGWHVHEKAILLAILPMSLLSVGKAGDASIFLILTTTGHYSLFPLLFTAPELP 432

Query: 523 IKVTLLLLHCVLMWFGFSSYFSGETTTNSNKHLQKKNYEDISDKGKLGVGVGVGWLCKSY 582
           IK+ L+LL      F   S  S +T     K L                     W+   Y
Sbjct: 433 IKILLMLL------FTIYSISSLKTLFRKEKPL-------------------FNWMETFY 492

Query: 583 LLGLVAVEIWGQFLHTL-FFGNKLPFLPLMLVSEALA---TYSVVWMWSTTICSSNLAAT 606
           LLGL  +E+  +F+     +  K PF+PL+L S   A   TY+   ++ + +  S +  T
Sbjct: 493 LLGLGPLEVCCEFVFPFTSWKVKYPFIPLLLTSVYCAVGITYAWFKLYVSVLIDSAIGKT 523

		  

BLAST of Spo11449.1 vs. ExPASy Swiss-Prot
Match: ALG8_MOUSE (Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Mus musculus GN=Alg8 PE=2 SV=2)

HSP 1 Score: 341.3 bits (874), Expect = 2.600e-92
Identity = 216/541 (39.93%), Postives = 296/541 (54.71%), Query Frame = 1

		  

Query: 103 WFSLFS---TSIKLLLLPSYRSTDFEVHRNWLATTHSLPLSHWYSDTTSQWTLDYPPFFA 162
           WFS  +   T +K LL+P+Y STDFEVHRNWLA THSLP+S WY + TS+WTLDYPPFFA
Sbjct: 13  WFSALALGVTLLKCLLIPTYHSTDFEVHRNWLAITHSLPISQWYYEATSEWTLDYPPFFA 72

Query: 163 YFELFLSFIAAQIDPAIVNLHEGLNYKADSVIYFQRITVIVSDLILHYAIY--------- 222
           +FE  LS IA   D  ++N+H  LNY +   + FQR +VI++D +  YA++         
Sbjct: 73  WFEYALSHIAKYFDQEMLNIH-NLNYYSSRTLLFQRFSVILTDALFVYAVHECCKCIDGK 132

Query: 223 RITRNLDSTKRRLIWILVIWSPALLIVDHMHFQYNGFLLGFLLISISALIEGRDLMGGFF 282
           R  ++L    + ++ +L++W+  LLIVDH+HFQYNGFL G LL+SI+ L + R + G   
Sbjct: 133 RTGKDLTEKPKFILSVLLLWNFGLLIVDHIHFQYNGFLSGLLLLSIARLFQKRHIEGALL 192

Query: 283 FAVLLCFKHLFAVAAPVYFVYLLRHYC------RGGVQHTP---------GSHQMMLRAL 342
           FAVLL  KH++   AP Y VYLLR YC       G V+ +          G    ++ AL
Sbjct: 193 FAVLLHLKHIYLYVAPAYGVYLLRSYCFTASKPDGSVRWSSFSVVRVTSLGLIVFLVSAL 252

Query: 343 MQNSSACMKML-QVIHRMFPFGRGLCHAFWAPNFWVFYIMADKLLAFFLRRMGF--NVQR 402
                  +  L QV  R+FPF RGLCHA+WAPNFW  Y   DK+L+    ++      Q 
Sbjct: 253 SLGPFLALNQLPQVFSRLFPFKRGLCHAYWAPNFWALYNALDKVLSVIGLKLKLLDPSQI 312

Query: 403 PAGSFTSGLVGDSSPFAVLPQITPLVTFIMVLLAISPCLFKAWKNPQ-PRKVARWITYAY 462
           P  S TSGLV       VLP ++PL T I  L+AI P +F  W  PQ PR   R +    
Sbjct: 313 PRASMTSGLV-QQFQHTVLPSVSPLATLICTLIAILPSVFCLWFKPQGPRGFLRCLVLCA 372

Query: 463 SCGFLFGWHVHEKATLHFLIPLAINAVDSINDARHYFLLSIVSCYSLFPLLFETREYPIK 522
              F+FGWHVHEKA L  ++P+++ +V+   DA  + +L+    YSLFPLLF   E PIK
Sbjct: 373 LSSFMFGWHVHEKAILLAILPMSLLSVEKAGDATVFLILATTGHYSLFPLLFTAPELPIK 432

Query: 523 VTLLLLHCVLMWFGFSSYFSGETTTNSNKHLQKKNYEDISDKGKLGVGVGVGWLCKSYLL 582
           + L+LL      F   S  S +T     K L                     W+   YLL
Sbjct: 433 ILLMLL------FTVYSISSLKTLFRKEKPL-------------------FNWMETVYLL 492

Query: 583 GLVAVEIWGQFLHTL-FFGNKLPFLPLMLVSEALATYSVVWMWS---TTICSSNLAATTH 609
           GL  +E+  +FL     +  K PF+PL+L S   A   + + W+    ++ + +L + T 
Sbjct: 493 GLGPLEVCCEFLLPFTSWKLKYPFIPLLLTSVYCAV-GITYAWTRLYASVLTGSLVSKTK 525

		  

BLAST of Spo11449.1 vs. ExPASy Swiss-Prot
Match: ALG8_BOVIN (Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Bos taurus GN=ALG8 PE=2 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 4.800e-91
Identity = 211/524 (40.27%), Postives = 286/524 (54.58%), Query Frame = 1

		  

Query: 103 WFSLFS---TSIKLLLLPSYRSTDFEVHRNWLATTHSLPLSHWYSDTTSQWTLDYPPFFA 162
           WFS  +   T +K LL+P+Y STDFEVHRNWLA THSLP+S WY + TS+WTLDYPPFFA
Sbjct: 13  WFSALALGVTLLKCLLIPTYHSTDFEVHRNWLAITHSLPISQWYYEATSEWTLDYPPFFA 72

Query: 163 YFELFLSFIAAQIDPAIVNLHEGLNYKADSVIYFQRITVIVSDLILHYAIYRITRNLDST 222
           +FE  LS +A   D  ++N+   LNY +   + FQR +VI +D +  YA++   + +D  
Sbjct: 73  WFEYALSHVAKYFDQEMLNV-RNLNYSSSRTLLFQRFSVIFTDALFVYAVHECCKCIDGK 132

Query: 223 K---------RRLIWILVIWSPALLIVDHMHFQYNGFLLGFLLISISALIEGRDLMGGFF 282
           K         + ++  L++W+  LLIVDH+HFQYNGFL G +L+SI+   + R + G F 
Sbjct: 133 KAGKELTEKPKFILSALLLWNFGLLIVDHIHFQYNGFLSGLMLLSIARFFQKRHMEGAFL 192

Query: 283 FAVLLCFKHLFAVAAPVYFVYLLRHYC------RGGVQHTP---------GSHQMMLRAL 342
           FAVLL FKH++   AP Y VYLLR YC       G ++            G    ++ AL
Sbjct: 193 FAVLLHFKHIYLYVAPAYGVYLLRSYCFTANKQDGSIRWNSFSFVRLISLGLIVFLVSAL 252

Query: 343 MQNSSACMKML-QVIHRMFPFGRGLCHAFWAPNFWVFYIMADKLLAFF---LRRMGFNVQ 402
                  +  L QV  R+FPF RGLCHA+WAPNFW  Y   DK+L+     L+ +  N +
Sbjct: 253 SLGPFLALNQLPQVFSRLFPFKRGLCHAYWAPNFWALYSAFDKVLSVIGLELKLLDPN-K 312

Query: 403 RPAGSFTSGLVGDSSPFAVLPQITPLVTFIMVLLAISPCLFKAWKNPQ-PRKVARWITYA 462
            P  S TSGLV       VLP +TPL TFI  L+A+ P +F  W  PQ PR   R +   
Sbjct: 313 IPKASMTSGLV-QQFQHTVLPSVTPLATFICTLIAMLPSVFCLWCKPQGPRGFLRCLILC 372

Query: 463 YSCGFLFGWHVHEKATLHFLIPLAINAVDSINDARHYFLLSIVSCYSLFPLLFETREYPI 522
               F+FGWHVHEKA L  ++P+++ +V    DA  + +L+    YS FPLLF   E PI
Sbjct: 373 ALSSFMFGWHVHEKAILLAVLPMSLLSVGKAVDASIFLILTTTGHYSPFPLLFTAPELPI 432

Query: 523 KVTLLLLHCVLMWFGFSSYFSGETTTNSNKHLQKKNYEDISDKGKLGVGVGVGWLCKSYL 582
           K+ L+LL      F   S  S +T     K L                     W+   YL
Sbjct: 433 KIILMLL------FTIYSISSLKTLFRKEKPL-------------------FNWMETFYL 492

Query: 583 LGLVAVEIWGQFLHTLFFGN-KLPFLPLMLVSEALATYSVVWMW 594
           LGL  +E++ +F+      N K PF+PL+L S   A   + + W
Sbjct: 493 LGLGPLEVFCEFVFPFTSWNLKYPFIPLLLTSVYCAV-GITYAW 507

		  

BLAST of Spo11449.1 vs. ExPASy Swiss-Prot
Match: ALG8_DROME (Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase OS=Drosophila melanogaster GN=xit PE=2 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 9.400e-87
Identity = 200/500 (40.00%), Postives = 287/500 (57.40%), Query Frame = 1

		  

Query: 98  IKEILWFSL-FSTSIKLLLLPSYRSTDFEVHRNWLATTHSLPLSHWYSDTTSQWTLDYPP 157
           +K++ W  +  +T +K+LL+P+Y STDFEVHRNWLA THSLPL+ WY D TS+WTLDYPP
Sbjct: 1   MKDLFWHLVGIATGLKILLIPAYHSTDFEVHRNWLAITHSLPLNQWYVDATSEWTLDYPP 60

Query: 158 FFAYFELFLSFIAAQIDPAIVNLHEGLNYKADSVIYFQRITVIVSDLILHYAIYRITRNL 217
           FFAYFE  LS +A  +DP ++ + + LNY++ + +YFQR++VIV+DL+    +     +L
Sbjct: 61  FFAYFEWLLSQVAKYVDPRMLVV-DNLNYESKATVYFQRLSVIVTDLVYVLGVRSCLGSL 120

Query: 218 ----DSTKRRLIWILVIWSPALLIVDHMHFQYNGFLLGFLLISISALIEGRDLMGGFFFA 277
               D+ +     +L++ +  L+ VDH+HFQYNG L G LL+SI +LI  R L   F FA
Sbjct: 121 GLGRDTQQFFAASMLLLLNVGLIFVDHIHFQYNGLLFGILLLSIGSLIRQRFLWSAFAFA 180

Query: 278 VLLCFKHLFAVAAPVYFVYLLRHYC--RGGVQHTPGSHQMMLRALMQNSSACM-----KM 337
           VLL FKH+F   AP + VYLLR YC  +  V    G+   +L   +   +        ++
Sbjct: 181 VLLNFKHIFLYMAPAFGVYLLRFYCLEQASVVSAVGAVVKLLVVGLTPFAVSFGPFWQQL 240

Query: 338 LQVIHRMFPFGRGLCHAFWAPNFWVFYIMADKLLAFFLRRMGFNVQRPAGSFTSGLVGDS 397
            QV+ R+FPF RGL HA+WAPNFW  Y  ADKL A  L+     VQ    S TSGLV + 
Sbjct: 241 PQVLSRLFPFKRGLTHAYWAPNFWALYNAADKLAAGVLK-----VQDGGASTTSGLVQEV 300

Query: 398 SPFAVLPQITPLVTFIMVLLAISPCLFKAWKNPQPRKVARWITYAYSCG---FLFGWHVH 457
              +VLP ITP VTF +  L + P L K +++ + +    ++     CG   F+FGWHVH
Sbjct: 301 R-HSVLPAITPPVTFALTALFMLPILVKLFRSAKKQSPLVFLRAVVLCGCSSFVFGWHVH 360

Query: 458 EKATLHFLIPLAINAVDSINDARHYFLLSIVSCYSLFPLLFETREYPIKVTLLLLHCVLM 517
           EKA L  L+PL +  + +  DAR+ ++L I   +SLFPLLF+   Y  + +L + +  +M
Sbjct: 361 EKAILMVLLPLCLLTLVNREDARYAYVLGIAGYFSLFPLLFDADLYIPRYSLYMSYVAMM 420

Query: 518 WFGFSSYFSGETTTNSNKHLQKKNYEDISDKGKLGVGVGVGWLCKSYLLGLVAVEIWGQF 577
           +      F G    ++                       + WL   Y+LG +A+ ++   
Sbjct: 421 YGQLYRIFPGFRGFHT-----------------------LEWL---YMLGFMAIPLYEHL 467

Query: 578 L-HTLFFGNKLPFLPLMLVS 582
           L   L    +LPFLPL+L S
Sbjct: 481 LSFLLHLDQRLPFLPLLLTS 467

		  

BLAST of Spo11449.1 vs. TAIR (Arabidopsis)
Match: AT2G44660.1 (ALG6, ALG8 glycosyltransferase family)

HSP 1 Score: 627.1 bits (1616), Expect = 1.300e-179
Identity = 321/501 (64.07%), Postives = 379/501 (75.65%), Query Frame = 1

		  

Query: 101 ILWFSLFSTSIKLLLLPSYRSTDFEVHRNWLATTHSLPLSHWYSDTTSQWTLDYPPFFAY 160
           +LWF   +T++KLLL+PS RSTDFEVHRNWLA T+SLPL+ WY D TSQWTLDYPPFFAY
Sbjct: 12  LLWFFAVATAVKLLLIPSSRSTDFEVHRNWLAITNSLPLTKWYFDETSQWTLDYPPFFAY 71

Query: 161 FELFLSFIAAQIDPAIVNLHEGLNYKADSVIYFQRITVIVSDLILHYAIYRITRNLDSTK 220
           FE FLS  A  +DP IV+L  GL+Y A+SVIYFQRI+VIVSDL L Y +YR+TR L+  K
Sbjct: 72  FERFLSIFARLVDPRIVDLQSGLDYNAESVIYFQRISVIVSDLCLLYGVYRLTRKLEPLK 131

Query: 221 RRLIWILVIWSPALLIVDHMHFQYNGFLLGFLLISISALIEGRDLMGGFFFAVLLCFKHL 280
           R LI  LVIWSP LLIVDH+HFQYNGFLLG+LL+SIS L EGRDL+GGF FAVLLCFKHL
Sbjct: 132 RNLICALVIWSPGLLIVDHIHFQYNGFLLGWLLLSISFLQEGRDLLGGFLFAVLLCFKHL 191

Query: 281 FAVAAPVYFVYLLRHYCRGGVQHTPGSHQMMLRALMQNSSACM--------KMLQVIHRM 340
           FAV APVYFVYLLRHYC  G+  T     + + A++    A          ++ QVI RM
Sbjct: 192 FAVTAPVYFVYLLRHYCWSGLV-TGFRRLVTIGAVVVAVFAAAYGPFIYHGQIQQVISRM 251

Query: 341 FPFGRGLCHAFWAPNFWVFYIMADKLLAFFLRRMGFNVQRPAGSFTSGLVGDSSPFAVLP 400
           FPFGRGLCHA+WAPNFWVFYI+ DK LA  LR++G+ +Q P+ SFT GLVGDSSPFAVLP
Sbjct: 252 FPFGRGLCHAYWAPNFWVFYIILDKGLAVLLRKLGYEIQIPSASFTGGLVGDSSPFAVLP 311

Query: 401 QITPLVTFIMVLLAISPCLFKAWKNPQPRKVARWITYAYSCGFLFGWHVHEKATLHFLIP 460
           QITPL TF MVLLAISPCL KAWK P    VARW+ YAY+CGFLFGWHVHEKA+LHF IP
Sbjct: 312 QITPLTTFAMVLLAISPCLIKAWKKPHSGLVARWVAYAYTCGFLFGWHVHEKASLHFTIP 371

Query: 461 LAINAVDSINDARHYFLLSIVSCYSLFPLLFETREYPIKVTLLLLHCVLMWFGFSSYFSG 520
           LAI AV S+ DA+HYFL+SIVSCYSL+PLL+E +EYPIKV LLLLH V+MW GF++ ++ 
Sbjct: 372 LAIVAVQSLEDAKHYFLVSIVSCYSLYPLLYEPQEYPIKVLLLLLHSVVMWLGFAAQYT- 431

Query: 521 ETTTNSNKHLQKKNYEDISDKGKLGVGVGVGWLCKSYLLGLVAVEIWGQFLHTLFFGNKL 580
                 +   QKK   +I  K ++G         KSYL+GL+ VEI  QFLH  F G+KL
Sbjct: 432 ------DYKAQKKETSEIKSKFRIGC------FEKSYLMGLIIVEIVSQFLHPYFLGDKL 491

Query: 581 PFLPLMLVSEALATYSVVWMW 594
           PFLPLML+S    T  +++ W
Sbjct: 492 PFLPLMLIS-TYCTVGIMYSW 497

		  

BLAST of Spo11449.1 vs. TAIR (Arabidopsis)
Match: AT5G38460.1 (ALG6, ALG8 glycosyltransferase family)

HSP 1 Score: 77.8 bits (190), Expect = 3.000e-14
Identity = 69/260 (26.54%), Postives = 111/260 (42.69%), Query Frame = 1

		  

Query: 47  TPDTGYIFLYWAWTSSLSLSHISQNNSFFAISVFSFLPPSMEKQKQKPSKPIKEILWFSL 106
           +P TG     W W     L+H     SF  IS+F+ L  S              +  +  
Sbjct: 20  SPQTGSSIDTWWW-----LTHKGTTTSFLCISLFALLIRSA-------------VTMYPY 79

Query: 107 FSTSIKLLLLPSYRSTDFEVHRNWLATTHSLPLSHWYSDTT----SQWTLDYPPFFAYFE 166
               I     P +   DFE  R+W+  T +LP+  WY + T    + W LDYPP  AY  
Sbjct: 80  SGAGIP----PKFG--DFEAQRHWMEITTNLPVIDWYRNGTYNDLTYWGLDYPPLTAYQS 139

Query: 167 LFLSFIAAQIDPAIVNLHEGLNYKADSVIYFQRITVIVSDLILHYA-------IYRITRN 226
                     +P  V L     +++       R TV+ SD  + +        +Y   R 
Sbjct: 140 YIHGIFLRFFNPESVALLSSRGHESYLGKLLMRWTVLSSDAFIFFPAALFFVLVYHRNRT 199

Query: 227 LDSTKRRLIW--ILVIWSPALLIVDHMHFQYNGFLLGFLLISISALIEGRDLMGGFFFAV 286
               K  + W   +++ +P L+++DH HFQYN   LG  + +I+A++   +++    F++
Sbjct: 200 RGG-KSEVAWHIAMILLNPCLILIDHGHFQYNCISLGLTVGAIAAVLCESEVLTCVLFSL 254

Query: 287 LLCFKHLFAVAAPVYFVYLL 294
            L  K + A  AP +F +LL
Sbjct: 260 ALSHKQMSAYFAPAFFSHLL 254