Spo11585 (gene)

BLAST of Spo11585.1 vs. NCBI nr
Match: gi|902119190|gb|KNA03962.1| (hypothetical protein SOVF_204210 [Spinacia oleracea])

HSP 1 Score: 694.5 bits (1791), Expect = 1.100e-196
Identity = 373/374 (99.73%), Postives = 373/374 (99.73%), Query Frame = 1

		  

Query: 30  MEDSTGEQTTISADTAAALSLETTITTLPPQPESQPLSKRAQKKIIKQERYEVKKAEKKA 89
           MEDSTGEQTTISADTAAALSLETTITTLPPQPESQPLSKRAQKKIIKQERYEVKKAEKKA
Sbjct: 1   MEDSTGEQTTISADTAAALSLETTITTLPPQPESQPLSKRAQKKIIKQERYEVKKAEKKA 60

Query: 90  EVKEHKRRENERKRREWDEKLANLSSEEEKLKLIESKREMRKERMEQRSEERENKIQRLT 149
           EVKEHKRRENERKRREWDEKLANLSSEEEKLKLIESKREMRKERMEQRSEERENKIQRLT
Sbjct: 61  EVKEHKRRENERKRREWDEKLANLSSEEEKLKLIESKREMRKERMEQRSEERENKIQRLT 120

Query: 150 QGKLHGQNIVIDLEFSNLMKPNELNSLVQQIMYCYAVNSRCTSPGHLWLTGCEGNMGDHL 209
            GKLHGQNIVIDLEFSNLMKPNELNSLVQQIMYCYAVNSRCTSPGHLWLTGCEGNMGDHL
Sbjct: 121 HGKLHGQNIVIDLEFSNLMKPNELNSLVQQIMYCYAVNSRCTSPGHLWLTGCEGNMGDHL 180

Query: 210 NRLPGFDKWIIEKENRSYIEALQEQKENLVYLTADAETVLDDLDPKKIYIIGGLVDRNRW 269
           NRLPGFDKWIIEKENRSYIEALQEQKENLVYLTADAETVLDDLDPKKIYIIGGLVDRNRW
Sbjct: 181 NRLPGFDKWIIEKENRSYIEALQEQKENLVYLTADAETVLDDLDPKKIYIIGGLVDRNRW 240

Query: 270 KGLTMKKAEEQGIKAAKLPIGNYLKMSSSQVLTVNQVVEILLKYLEMKDWKASFFQVIPQ 329
           KGLTMKKAEEQGIKAAKLPIGNYLKMSSSQVLTVNQVVEILLKYLEMKDWKASFFQVIPQ
Sbjct: 241 KGLTMKKAEEQGIKAAKLPIGNYLKMSSSQVLTVNQVVEILLKYLEMKDWKASFFQVIPQ 300

Query: 330 RKRCDADSGDPEREIEDKEKTEDVSCTKNQKKMKLSETDSEGSEREVEIGEHEKSKSKPR 389
           RKRCDADSGDPEREIEDKEKTEDVSCTKNQKKMKLSETDSEGSEREVEIGEHEKSKSKPR
Sbjct: 301 RKRCDADSGDPEREIEDKEKTEDVSCTKNQKKMKLSETDSEGSEREVEIGEHEKSKSKPR 360

Query: 390 EDVESESQKPVEGK 404
           EDVESESQKPVEGK
Sbjct: 361 EDVESESQKPVEGK 374

		  

BLAST of Spo11585.1 vs. NCBI nr
Match: gi|731352026|ref|XP_010687325.1| (PREDICTED: tRNA (guanine(9)-N1)-methyltransferase [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 493.8 bits (1270), Expect = 2.800e-136
Identity = 265/338 (78.40%), Postives = 297/338 (87.87%), Query Frame = 1

		  

Query: 45  AAALSLETTITTLPPQPESQPLSKRAQKKIIKQERYEVKKAEKKAEVKEHKRRENERKRR 104
           A A    T  T+  P PE+Q LSKRAQKK++KQ++YEVKKAEKKAE KEHKRRE ERK++
Sbjct: 14  AIAAKPSTNTTSTSPSPETQTLSKRAQKKLLKQQKYEVKKAEKKAEAKEHKRREKERKQK 73

Query: 105 EWDEKLANLSSEEEKLKLIESKREMRKERMEQRSEERENKIQRLTQGKLHGQNIVIDLEF 164
           EW +KL+ +SSEEEKLKLIES+R +RKERME+RSEERE K+ RL+  K+HGQNIVIDLEF
Sbjct: 74  EWADKLSAISSEEEKLKLIESRRGVRKERMEKRSEEREKKLDRLSLAKIHGQNIVIDLEF 133

Query: 165 SNLMKPNELNSLVQQIMYCYAVNSRCTSPGHLWLTGCEGNMGDHLNRLPGFDKWIIEKEN 224
           S+LM PNELNSLVQQIMYCYAVN RCTSPGHLWLTGCEGNM  HL RLPGFDKWIIEKE 
Sbjct: 134 SHLMTPNELNSLVQQIMYCYAVNGRCTSPGHLWLTGCEGNMESHLQRLPGFDKWIIEKEK 193

Query: 225 RSYIEALQEQKENLVYLTADAETVLDDLDPKKIYIIGGLVDRNRWKGLTMKKAEEQGIKA 284
           RSYIEALQEQKE+LVYLTADAET+LDDLDPKKIYIIGGLVDRNRWKGLTMKKA+EQGI+A
Sbjct: 194 RSYIEALQEQKESLVYLTADAETILDDLDPKKIYIIGGLVDRNRWKGLTMKKAQEQGIQA 253

Query: 285 AKLPIGNYLKMSSSQVLTVNQVVEILLKYLEMKDWKASFFQVIPQRKRCDADSGDPEREI 344
           A+LPIGNYLKM SSQVLTVNQVVEILLKYLEM+DWK+SFFQVIPQRKRC+A S D  +E+
Sbjct: 254 ARLPIGNYLKMCSSQVLTVNQVVEILLKYLEMRDWKSSFFQVIPQRKRCEAGSDDHNKEL 313

Query: 345 EDKEKTEDVSCTKNQKKMKLSETDSEGSEREVEIGEHE 383
           ED+EKTED     +QKK+KLSETD E +EREV  GE E
Sbjct: 314 EDEEKTED----NSQKKIKLSETDMEANEREVVNGECE 347

		  

BLAST of Spo11585.1 vs. NCBI nr
Match: gi|731437668|ref|XP_010647426.1| (PREDICTED: tRNA (guanine(9)-N1)-methyltransferase-like [Vitis vinifera])

HSP 1 Score: 419.5 bits (1077), Expect = 6.800e-114
Identity = 225/316 (71.20%), Postives = 265/316 (83.86%), Query Frame = 1

		  

Query: 56  TLPPQPESQPLSKRAQKKIIKQERYEVKKAEKKAEVKEHKRRENERKRREWDEKLANLSS 115
           T  P P    LSK AQKK++KQ+R E KKAEKKA +KE K++E ERKR+EW+E LA++  
Sbjct: 13  TPAPPPPPLTLSKNAQKKLLKQQRMEAKKAEKKAAMKEQKKKEAERKRKEWEEMLASVD- 72

Query: 116 EEEKLKLIESKREMRKERMEQRSEERENKIQRLTQGKLHGQNIVIDLEFSNLMKPNELNS 175
           EEE+ KLIES++ +RKERME+RSEERE K+QRLT  K HGQNIV+DL+FS+LM PNE+NS
Sbjct: 73  EEERSKLIESRKGLRKERMEKRSEEREKKVQRLTNAKEHGQNIVVDLDFSDLMTPNEINS 132

Query: 176 LVQQIMYCYAVNSRCTSPGHLWLTGCEGNMGDHLNRLPGFDKWIIEKENRSYIEALQEQK 235
           LVQQIMYCYAVN RC SP HLWLTGC G MG  L RLPGFDKWIIEKEN+SYIEALQ++K
Sbjct: 133 LVQQIMYCYAVNRRCASPCHLWLTGCNGKMGSQLQRLPGFDKWIIEKENQSYIEALQDEK 192

Query: 236 ENLVYLTADAETVLDDLDPKKIYIIGGLVDRNRWKGLTMKKAEEQGIKAAKLPIGNYLKM 295
            NLVYLTAD+ET+LD+LDPKKIYI+GGLVDRNRWKG+TMKKA+EQGI+ AKLPIGNYLKM
Sbjct: 193 HNLVYLTADSETILDELDPKKIYIVGGLVDRNRWKGITMKKAQEQGIQTAKLPIGNYLKM 252

Query: 296 SSSQVLTVNQVVEILLKYLEMKDWKASFFQVIPQRKRCDADSGDPEREIEDKEKTEDVSC 355
           SSSQVLTVNQV+EILLK+LE KDWKASFFQVIPQRKR  ADS D + E+E+ E+ E+   
Sbjct: 253 SSSQVLTVNQVIEILLKFLETKDWKASFFQVIPQRKRGGADSEDCQGEVEEGEENEEGED 312

Query: 356 TKNQKKMKLSETDSEG 372
               KKM+ +E+ S G
Sbjct: 313 QLEMKKMR-TESPSNG 326

		  

BLAST of Spo11585.1 vs. NCBI nr
Match: gi|731439583|ref|XP_002270008.3| (PREDICTED: tRNA (guanine(9)-N1)-methyltransferase [Vitis vinifera])

HSP 1 Score: 417.5 bits (1072), Expect = 2.600e-113
Identity = 224/316 (70.89%), Postives = 265/316 (83.86%), Query Frame = 1

		  

Query: 56  TLPPQPESQPLSKRAQKKIIKQERYEVKKAEKKAEVKEHKRRENERKRREWDEKLANLSS 115
           T  P P    LSK AQKK++KQ+R E KKAEKKA +KE K++E ERKR+EW+E LA++  
Sbjct: 13  TPAPPPPPLTLSKNAQKKLLKQQRMEAKKAEKKAAMKEQKKKEAERKRKEWEEMLASVD- 72

Query: 116 EEEKLKLIESKREMRKERMEQRSEERENKIQRLTQGKLHGQNIVIDLEFSNLMKPNELNS 175
           EEE+ KLIES++ +RKERME+RSEERE K+QRLT  K HGQNIV+DL+FS+LM PNE+NS
Sbjct: 73  EEERSKLIESRKCLRKERMEKRSEEREKKVQRLTNAKEHGQNIVVDLDFSDLMTPNEINS 132

Query: 176 LVQQIMYCYAVNSRCTSPGHLWLTGCEGNMGDHLNRLPGFDKWIIEKENRSYIEALQEQK 235
           LVQQIMYCYAVN RC SP HLWLTGC G MG  L RLPGFDKWIIEKEN+SYIEALQ++K
Sbjct: 133 LVQQIMYCYAVNRRCASPCHLWLTGCNGKMGSQLQRLPGFDKWIIEKENQSYIEALQDEK 192

Query: 236 ENLVYLTADAETVLDDLDPKKIYIIGGLVDRNRWKGLTMKKAEEQGIKAAKLPIGNYLKM 295
            NLVYLTAD+ET+LD+LDPKKIYI+GGLVDRNRWKG+TMKKA+EQGI+ AKLPIGNYLKM
Sbjct: 193 HNLVYLTADSETILDELDPKKIYIVGGLVDRNRWKGITMKKAQEQGIQTAKLPIGNYLKM 252

Query: 296 SSSQVLTVNQVVEILLKYLEMKDWKASFFQVIPQRKRCDADSGDPEREIEDKEKTEDVSC 355
           SSSQVLTVNQV+EILLK+LE KDW+ASFFQVIPQRKR  ADS D + E+E+ E+ E+   
Sbjct: 253 SSSQVLTVNQVIEILLKFLETKDWRASFFQVIPQRKRGGADSEDCQGEVEEGEENEEGED 312

Query: 356 TKNQKKMKLSETDSEG 372
               KKM+ +E+ S G
Sbjct: 313 QLEMKKMR-TESPSNG 326

		  

BLAST of Spo11585.1 vs. NCBI nr
Match: gi|802614729|ref|XP_012074807.1| (PREDICTED: tRNA (guanine(9)-N1)-methyltransferase [Jatropha curcas])

HSP 1 Score: 415.6 bits (1067), Expect = 9.800e-113
Identity = 227/342 (66.37%), Postives = 276/342 (80.70%), Query Frame = 1

		  

Query: 20  QTAETPTQTEMEDSTGEQTTISADTAAALSLETTITTLPPQPESQPLSKRAQKKIIKQER 79
           + + T + T + D T  + T   + +    +       PP P+ QPLSK AQKK++KQ++
Sbjct: 22  EVSATASATMVADDTQIEQTTLDNRSDLQPIPPQDEQQPPSPQPQPLSKNAQKKLLKQQK 81

Query: 80  YEVKKAEKKAEVKEHKRRENERKRREWDEKLANLSSEEEKLKLIESKREMRKERMEQRSE 139
           +E KKAEKKA +KE K++E ERKR+EW+E LA +S EEE+ KLIES+RE+RKERME+RSE
Sbjct: 82  FEAKKAEKKALMKEQKKKEAERKRKEWEETLAGVS-EEEREKLIESRRELRKERMEKRSE 141

Query: 140 ERENKIQRLTQGKLHGQNIVIDLEFSNLMKPNELNSLVQQIMYCYAVNSRCTSPGHLWLT 199
           ERENKIQRLT+ K +GQ IVIDLEFS+LM  +E++SLVQQIMYCYAVN RC +PG LWLT
Sbjct: 142 ERENKIQRLTEAKSNGQKIVIDLEFSHLMTSSEIHSLVQQIMYCYAVNGRCATPGLLWLT 201

Query: 200 GCEGNMGDHLNRLPGFDKWIIEKENRSYIEALQEQKENLVYLTADAETVLDDLDPKKIYI 259
           GC G M   L RLPGFDKWIIEKE+RSYIEALQ+QKENLVYLTADAET LD+LD K+IYI
Sbjct: 202 GCAGEMESQLQRLPGFDKWIIEKESRSYIEALQDQKENLVYLTADAETELDELDLKRIYI 261

Query: 260 IGGLVDRNRWKGLTMKKAEEQGIKAAKLPIGNYLKMSSSQVLTVNQVVEILLKYLEMKDW 319
           +GGLVDRNRWKGLTMKKA+EQGI+ AKLPIGN+LKMSSSQVLTVNQV+EILLK+LE KDW
Sbjct: 262 VGGLVDRNRWKGLTMKKAQEQGIQTAKLPIGNHLKMSSSQVLTVNQVIEILLKFLETKDW 321

Query: 320 KASFFQVIPQRKRCDADSGDPEREIEDKEKTEDVSCTKNQKK 362
           K SFFQVIPQRKRCDADS +  R ++++E  E     +N+K+
Sbjct: 322 KDSFFQVIPQRKRCDADSTEHHRGVDEEENEEKEESCENKKR 362

		  

BLAST of Spo11585.1 vs. UniProtKB/TrEMBL
Match: A0A0K9Q9J7_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_204210 PE=4 SV=1)

HSP 1 Score: 694.5 bits (1791), Expect = 7.600e-197
Identity = 373/374 (99.73%), Postives = 373/374 (99.73%), Query Frame = 1

		  

Query: 30  MEDSTGEQTTISADTAAALSLETTITTLPPQPESQPLSKRAQKKIIKQERYEVKKAEKKA 89
           MEDSTGEQTTISADTAAALSLETTITTLPPQPESQPLSKRAQKKIIKQERYEVKKAEKKA
Sbjct: 1   MEDSTGEQTTISADTAAALSLETTITTLPPQPESQPLSKRAQKKIIKQERYEVKKAEKKA 60

Query: 90  EVKEHKRRENERKRREWDEKLANLSSEEEKLKLIESKREMRKERMEQRSEERENKIQRLT 149
           EVKEHKRRENERKRREWDEKLANLSSEEEKLKLIESKREMRKERMEQRSEERENKIQRLT
Sbjct: 61  EVKEHKRRENERKRREWDEKLANLSSEEEKLKLIESKREMRKERMEQRSEERENKIQRLT 120

Query: 150 QGKLHGQNIVIDLEFSNLMKPNELNSLVQQIMYCYAVNSRCTSPGHLWLTGCEGNMGDHL 209
            GKLHGQNIVIDLEFSNLMKPNELNSLVQQIMYCYAVNSRCTSPGHLWLTGCEGNMGDHL
Sbjct: 121 HGKLHGQNIVIDLEFSNLMKPNELNSLVQQIMYCYAVNSRCTSPGHLWLTGCEGNMGDHL 180

Query: 210 NRLPGFDKWIIEKENRSYIEALQEQKENLVYLTADAETVLDDLDPKKIYIIGGLVDRNRW 269
           NRLPGFDKWIIEKENRSYIEALQEQKENLVYLTADAETVLDDLDPKKIYIIGGLVDRNRW
Sbjct: 181 NRLPGFDKWIIEKENRSYIEALQEQKENLVYLTADAETVLDDLDPKKIYIIGGLVDRNRW 240

Query: 270 KGLTMKKAEEQGIKAAKLPIGNYLKMSSSQVLTVNQVVEILLKYLEMKDWKASFFQVIPQ 329
           KGLTMKKAEEQGIKAAKLPIGNYLKMSSSQVLTVNQVVEILLKYLEMKDWKASFFQVIPQ
Sbjct: 241 KGLTMKKAEEQGIKAAKLPIGNYLKMSSSQVLTVNQVVEILLKYLEMKDWKASFFQVIPQ 300

Query: 330 RKRCDADSGDPEREIEDKEKTEDVSCTKNQKKMKLSETDSEGSEREVEIGEHEKSKSKPR 389
           RKRCDADSGDPEREIEDKEKTEDVSCTKNQKKMKLSETDSEGSEREVEIGEHEKSKSKPR
Sbjct: 301 RKRCDADSGDPEREIEDKEKTEDVSCTKNQKKMKLSETDSEGSEREVEIGEHEKSKSKPR 360

Query: 390 EDVESESQKPVEGK 404
           EDVESESQKPVEGK
Sbjct: 361 EDVESESQKPVEGK 374

		  

BLAST of Spo11585.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BQL8_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_8g189400 PE=4 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 2.000e-136
Identity = 265/338 (78.40%), Postives = 297/338 (87.87%), Query Frame = 1

		  

Query: 45  AAALSLETTITTLPPQPESQPLSKRAQKKIIKQERYEVKKAEKKAEVKEHKRRENERKRR 104
           A A    T  T+  P PE+Q LSKRAQKK++KQ++YEVKKAEKKAE KEHKRRE ERK++
Sbjct: 14  AIAAKPSTNTTSTSPSPETQTLSKRAQKKLLKQQKYEVKKAEKKAEAKEHKRREKERKQK 73

Query: 105 EWDEKLANLSSEEEKLKLIESKREMRKERMEQRSEERENKIQRLTQGKLHGQNIVIDLEF 164
           EW +KL+ +SSEEEKLKLIES+R +RKERME+RSEERE K+ RL+  K+HGQNIVIDLEF
Sbjct: 74  EWADKLSAISSEEEKLKLIESRRGVRKERMEKRSEEREKKLDRLSLAKIHGQNIVIDLEF 133

Query: 165 SNLMKPNELNSLVQQIMYCYAVNSRCTSPGHLWLTGCEGNMGDHLNRLPGFDKWIIEKEN 224
           S+LM PNELNSLVQQIMYCYAVN RCTSPGHLWLTGCEGNM  HL RLPGFDKWIIEKE 
Sbjct: 134 SHLMTPNELNSLVQQIMYCYAVNGRCTSPGHLWLTGCEGNMESHLQRLPGFDKWIIEKEK 193

Query: 225 RSYIEALQEQKENLVYLTADAETVLDDLDPKKIYIIGGLVDRNRWKGLTMKKAEEQGIKA 284
           RSYIEALQEQKE+LVYLTADAET+LDDLDPKKIYIIGGLVDRNRWKGLTMKKA+EQGI+A
Sbjct: 194 RSYIEALQEQKESLVYLTADAETILDDLDPKKIYIIGGLVDRNRWKGLTMKKAQEQGIQA 253

Query: 285 AKLPIGNYLKMSSSQVLTVNQVVEILLKYLEMKDWKASFFQVIPQRKRCDADSGDPEREI 344
           A+LPIGNYLKM SSQVLTVNQVVEILLKYLEM+DWK+SFFQVIPQRKRC+A S D  +E+
Sbjct: 254 ARLPIGNYLKMCSSQVLTVNQVVEILLKYLEMRDWKSSFFQVIPQRKRCEAGSDDHNKEL 313

Query: 345 EDKEKTEDVSCTKNQKKMKLSETDSEGSEREVEIGEHE 383
           ED+EKTED     +QKK+KLSETD E +EREV  GE E
Sbjct: 314 EDEEKTED----NSQKKIKLSETDMEANEREVVNGECE 347

		  

BLAST of Spo11585.1 vs. UniProtKB/TrEMBL
Match: A5AJA8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_030149 PE=4 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 4.700e-114
Identity = 225/316 (71.20%), Postives = 265/316 (83.86%), Query Frame = 1

		  

Query: 56  TLPPQPESQPLSKRAQKKIIKQERYEVKKAEKKAEVKEHKRRENERKRREWDEKLANLSS 115
           T  P P    LSK AQKK++KQ+R E KKAEKKA +KE K++E ERKR+EW+E LA++  
Sbjct: 13  TPAPPPPPLTLSKNAQKKLLKQQRMEAKKAEKKAAMKEQKKKEAERKRKEWEEMLASVD- 72

Query: 116 EEEKLKLIESKREMRKERMEQRSEERENKIQRLTQGKLHGQNIVIDLEFSNLMKPNELNS 175
           EEE+ KLIES++ +RKERME+RSEERE K+QRLT  K HGQNIV+DL+FS+LM PNE+NS
Sbjct: 73  EEERSKLIESRKGLRKERMEKRSEEREKKVQRLTNAKEHGQNIVVDLDFSDLMTPNEINS 132

Query: 176 LVQQIMYCYAVNSRCTSPGHLWLTGCEGNMGDHLNRLPGFDKWIIEKENRSYIEALQEQK 235
           LVQQIMYCYAVN RC SP HLWLTGC G MG  L RLPGFDKWIIEKEN+SYIEALQ++K
Sbjct: 133 LVQQIMYCYAVNRRCASPCHLWLTGCNGKMGSQLQRLPGFDKWIIEKENQSYIEALQDEK 192

Query: 236 ENLVYLTADAETVLDDLDPKKIYIIGGLVDRNRWKGLTMKKAEEQGIKAAKLPIGNYLKM 295
            NLVYLTAD+ET+LD+LDPKKIYI+GGLVDRNRWKG+TMKKA+EQGI+ AKLPIGNYLKM
Sbjct: 193 HNLVYLTADSETILDELDPKKIYIVGGLVDRNRWKGITMKKAQEQGIQTAKLPIGNYLKM 252

Query: 296 SSSQVLTVNQVVEILLKYLEMKDWKASFFQVIPQRKRCDADSGDPEREIEDKEKTEDVSC 355
           SSSQVLTVNQV+EILLK+LE KDWKASFFQVIPQRKR  ADS D + E+E+ E+ E+   
Sbjct: 253 SSSQVLTVNQVIEILLKFLETKDWKASFFQVIPQRKRGGADSEDCQGEVEEGEENEEGED 312

Query: 356 TKNQKKMKLSETDSEG 372
               KKM+ +E+ S G
Sbjct: 313 QLEMKKMR-TESPSNG 326

		  

BLAST of Spo11585.1 vs. UniProtKB/TrEMBL
Match: A0A067KUI9_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_08957 PE=4 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 6.800e-113
Identity = 227/342 (66.37%), Postives = 276/342 (80.70%), Query Frame = 1

		  

Query: 20  QTAETPTQTEMEDSTGEQTTISADTAAALSLETTITTLPPQPESQPLSKRAQKKIIKQER 79
           + + T + T + D T  + T   + +    +       PP P+ QPLSK AQKK++KQ++
Sbjct: 22  EVSATASATMVADDTQIEQTTLDNRSDLQPIPPQDEQQPPSPQPQPLSKNAQKKLLKQQK 81

Query: 80  YEVKKAEKKAEVKEHKRRENERKRREWDEKLANLSSEEEKLKLIESKREMRKERMEQRSE 139
           +E KKAEKKA +KE K++E ERKR+EW+E LA +S EEE+ KLIES+RE+RKERME+RSE
Sbjct: 82  FEAKKAEKKALMKEQKKKEAERKRKEWEETLAGVS-EEEREKLIESRRELRKERMEKRSE 141

Query: 140 ERENKIQRLTQGKLHGQNIVIDLEFSNLMKPNELNSLVQQIMYCYAVNSRCTSPGHLWLT 199
           ERENKIQRLT+ K +GQ IVIDLEFS+LM  +E++SLVQQIMYCYAVN RC +PG LWLT
Sbjct: 142 ERENKIQRLTEAKSNGQKIVIDLEFSHLMTSSEIHSLVQQIMYCYAVNGRCATPGLLWLT 201

Query: 200 GCEGNMGDHLNRLPGFDKWIIEKENRSYIEALQEQKENLVYLTADAETVLDDLDPKKIYI 259
           GC G M   L RLPGFDKWIIEKE+RSYIEALQ+QKENLVYLTADAET LD+LD K+IYI
Sbjct: 202 GCAGEMESQLQRLPGFDKWIIEKESRSYIEALQDQKENLVYLTADAETELDELDLKRIYI 261

Query: 260 IGGLVDRNRWKGLTMKKAEEQGIKAAKLPIGNYLKMSSSQVLTVNQVVEILLKYLEMKDW 319
           +GGLVDRNRWKGLTMKKA+EQGI+ AKLPIGN+LKMSSSQVLTVNQV+EILLK+LE KDW
Sbjct: 262 VGGLVDRNRWKGLTMKKAQEQGIQTAKLPIGNHLKMSSSQVLTVNQVIEILLKFLETKDW 321

Query: 320 KASFFQVIPQRKRCDADSGDPEREIEDKEKTEDVSCTKNQKK 362
           K SFFQVIPQRKRCDADS +  R ++++E  E     +N+K+
Sbjct: 322 KDSFFQVIPQRKRCDADSTEHHRGVDEEENEEKEESCENKKR 362

		  

BLAST of Spo11585.1 vs. UniProtKB/TrEMBL
Match: B9HC28_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0006s00680g PE=4 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 3.700e-111
Identity = 219/314 (69.75%), Postives = 271/314 (86.31%), Query Frame = 1

		  

Query: 56  TLPPQPESQ--PLSKRAQKKIIKQERYEVKKAEKKAEVKEHKRRENERKRREWDEKLANL 115
           TL  QP SQ  PLSK AQKK++KQ+++E KKAEKKA+VKE K +E ERKR+EW+E+L++ 
Sbjct: 14  TLESQPNSQPTPLSKNAQKKLLKQQKWEAKKAEKKAQVKEQKMKEAERKRKEWEERLSSC 73

Query: 116 SSEEEKLKLIESKREMRKERMEQRSEERENKIQRLTQGKLHGQNIVIDLEFSNLMKPNEL 175
           +SEEE+LKLIES+RE+RKERME+R+EE+E+K+QRL++ K  GQNIVIDLEF++LM  +E+
Sbjct: 74  ASEEERLKLIESRRELRKERMEKRAEEKEDKVQRLSKAKEFGQNIVIDLEFADLMTNSEI 133

Query: 176 NSLVQQIMYCYAVNSRCTSPGHLWLTGCEGNMGDHLNRLPGFDKWIIEKENRSYIEALQE 235
           +SLVQQIMYCYAVN RCTSPGHLWLTGC G M + L RLPGFDKWIIEKE++SYI+ALQ+
Sbjct: 134 HSLVQQIMYCYAVNRRCTSPGHLWLTGCRGEMENQLQRLPGFDKWIIEKESQSYIDALQD 193

Query: 236 QKENLVYLTADAETVLDDLDPKKIYIIGGLVDRNRWKGLTMKKAEEQGIKAAKLPIGNYL 295
           QKENLVYLTAD+ETVLD+LD KKIYIIGGLVDRNRWKG+TMKKA EQGI+ AKLPIG+YL
Sbjct: 194 QKENLVYLTADSETVLDELDLKKIYIIGGLVDRNRWKGITMKKASEQGIQTAKLPIGSYL 253

Query: 296 KMSSSQVLTVNQVVEILLKYLEMKDWKASFFQVIPQRKRCDADSGDPEREIEDKEKTEDV 355
           KMSSSQVLTVNQVVEILLK++E+KDWKASFFQVIPQRKR + DS + +    ++ + ED 
Sbjct: 254 KMSSSQVLTVNQVVEILLKFVEIKDWKASFFQVIPQRKRGETDSEELQGVDGEELENEDD 313

Query: 356 SCTKNQKKMKLSET 368
              K ++ +++  T
Sbjct: 314 PSDKKKRCIEVRST 327

		  

BLAST of Spo11585.1 vs. ExPASy Swiss-Prot
Match: TM10A_HUMAN (tRNA methyltransferase 10 homolog A OS=Homo sapiens GN=TRMT10A PE=1 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 5.900e-33
Identity = 113/340 (33.24%), Postives = 186/340 (54.71%), Query Frame = 1

		  

Query: 64  QPLSKRAQKKIIKQERYEVKKAEKKAEVKEHKRRENERKRREWDEKLANLSSEEEKLKLI 123
           +P+SKR  KK+IKQ+++E ++     E+++ KR+E +RKR++ +                
Sbjct: 38  EPISKRQMKKLIKQKQWEEQR-----ELRKQKRKE-KRKRKKLE---------------- 97

Query: 124 ESKREMRKERMEQRSEERENKIQRLTQGKLHGQ-NIVIDLEFSNLMKPNELNSLVQQIMY 183
                 R+ +ME  S+  + K  R+ +  +H    ++ID  F +LM   ++  L +QI  
Sbjct: 98  ------RQCQMEPNSDGHDRK--RVRRDVVHSTLRLIIDCSFDHLMVLKDIKKLHKQIQR 157

Query: 184 CYAVNSRCTSPGHLWLTGCEGNMGDHLNRLP-GFDKWI-IEKENRSYIEALQEQKENLVY 243
           CYA N R   P   +LT   G +  +++    G+  W  I  +   Y E ++  KE+L+Y
Sbjct: 158 CYAENRRALHPVQFYLTSHGGQLKKNMDENDKGWVNWKDIHIKPEHYSELIK--KEDLIY 217

Query: 244 LTADAETVLDDLDPKKIYIIGGLVDRNRWKGLTMKKAEEQGIKAAKLPIGNYLKMSSSQV 303
           LT+D+  +L +LD  K Y+IGGLVD N  KGLT K+A + GI  A+LP+GN++KM+S +V
Sbjct: 218 LTSDSPNILKELDESKAYVIGGLVDHNHHKGLTYKQASDYGINHAQLPLGNFVKMNSRKV 277

Query: 304 LTVNQVVEILLKYLEMKDWKASFFQVIPQRKRCDADSGDPEREIEDKEKTEDVSCTKNQK 363
           L VN V EI+L+YLE +DW+ +FF ++PQRK            +   +  E  S      
Sbjct: 278 LAVNHVFEIILEYLETRDWQEAFFTILPQRKGA----------VPTDKACESASHDNQSV 334

Query: 364 KMKLSETDSEGSEREVEIGEHEKSKSKPREDVESESQKPV 401
           +M+   +DS+ SE E    E + S  + ++D E+ ++  V
Sbjct: 338 RMEEGGSDSDSSEEEYSRNELD-SPHEEKQDKENHTESTV 334

		  

BLAST of Spo11585.1 vs. ExPASy Swiss-Prot
Match: TM10A_XENTR (tRNA methyltransferase 10 homolog A OS=Xenopus tropicalis GN=trmt10a PE=2 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 1.000e-32
Identity = 109/293 (37.20%), Postives = 165/293 (56.31%), Query Frame = 1

		  

Query: 66  LSKRAQKKIIKQERYEVKKAEKKAEVKEHKRRENERKRREWDEKLANLSSEEEKLKLIES 125
           +SKR  KK +KQ+++E ++     E+++ KR+E  +KR     KL   +  E  +     
Sbjct: 50  MSKRQMKKFLKQKQWEDQR-----ELRKQKRKEKRQKR-----KLERQAQAEHNIDANSR 109

Query: 126 KREMRKERMEQRSEERENKIQRLTQGKLHGQNIVIDLEFSNLMKPNELNSLVQQIMYCYA 185
           KR         R E + + ++           ++ID  F +LM   ++  L +QI  CYA
Sbjct: 110 KR--------FRHEVQPSALR-----------LIIDCSFDDLMALRDVKKLNKQIRRCYA 169

Query: 186 VNSRCTSPGHLWLTGCEGNMGDHLNRLPGFDK-WIIEKENRSYIEALQE--QKENLVYLT 245
            N R   P  L+LT   G +  +++    +DK WI  K+     E  ++  +KE+LVYLT
Sbjct: 170 ENRRAVHPVQLYLTSHGGQLKSNMDE---YDKGWINWKDIHIKPEHYKDLIKKEDLVYLT 229

Query: 246 ADAETVLDDLDPKKIYIIGGLVDRNRWKGLTMKKAEEQGIKAAKLPIGNYLKMSSSQVLT 305
           +D+  VL +LD  K YIIGGLVD N  KG+T KKA E GI  A+LP+GN++KM++ +VL 
Sbjct: 230 SDSPEVLSELDETKAYIIGGLVDHNHHKGITYKKALELGISHAQLPLGNFVKMNTRKVLA 289

Query: 306 VNQVVEILLKYLEMKDWKASFFQVIPQRKRCD--ADSGD-PEREIEDKEKTED 353
           VN V EI+L +LE K+WK +FF V+PQRK      ++G+ PE    ++E  ED
Sbjct: 290 VNHVFEIILAFLEKKEWKEAFFSVLPQRKGAIPLTETGEQPECRASEQEDGED 310

		  

BLAST of Spo11585.1 vs. ExPASy Swiss-Prot
Match: TRM10_SCHPO (tRNA (guanine(9)-N1)-methyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=trm10 PE=1 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 5.000e-32
Identity = 107/309 (34.63%), Postives = 161/309 (52.10%), Query Frame = 1

		  

Query: 77  QERYEVKKAEKKAEVKEHKRRENERKRREWDEKLANLSSEEEKLKLIESKREMRKERMEQ 136
           ++  ++ K +      +  + E + +       L  L  ++E     E + EMR+E+   
Sbjct: 4   KDALDIGKDDTNTSEADVSKNETQEQPVLSKSALKRLKRQQEWDAGREKRAEMRREKKRL 63

Query: 137 RSEERENKIQ----------RLTQGKLHGQNI--VIDLEFSNLMKPNELNSLVQQIMYCY 196
           R EER+ KI+          R+  GK+   +I  V+D  F +LM   E+NSL QQ+  C+
Sbjct: 64  RKEERKRKIEAGEVVKSQKKRIRLGKVVPSSIRIVLDCAFDDLMNDKEINSLCQQVTRCH 123

Query: 197 AVNSRCTSPGHLWLTGCEGNMGDHLNRLPGF------DKWI-IEKENRSYIEALQEQKEN 256
           + N     P  L+ T    N G  L     F      + W       +SY+E  + QKE 
Sbjct: 124 SANRTALHPVELFAT----NFGGRLKTRQDFVLKGQQNNWKRYNPTTKSYLEEFESQKEK 183

Query: 257 LVYLTADAETVLDDLDPKKIYIIGGLVDRNRWKGLTMKKAEEQGIKAAKLPIGNYLKMSS 316
           LVYL+AD++  + +LD  KIYIIG +VD+NR+K L   KA EQGIK AKLPI  Y+K++ 
Sbjct: 184 LVYLSADSDNTITELDEDKIYIIGAIVDKNRYKNLCQNKASEQGIKTAKLPIDEYIKITD 243

Query: 317 SQVLTVNQVVEILLKYLEMKDWKASFFQVIPQRKRCDADSGDPEREIEDKEKTEDVSCTK 367
            ++LTVNQV EIL  +LE +DW+ +F +VIP+RK      G   +  E  + +ED     
Sbjct: 244 RKILTVNQVFEILSLWLEYRDWEKAFMEVIPKRK------GILLKSDESFDVSEDTRSQS 302

		  

BLAST of Spo11585.1 vs. ExPASy Swiss-Prot
Match: TM10A_BOVIN (tRNA methyltransferase 10 homolog A OS=Bos taurus GN=TRMT10A PE=2 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 2.500e-31
Identity = 113/337 (33.53%), Postives = 178/337 (52.82%), Query Frame = 1

		  

Query: 64  QPLSKRAQKKIIKQERYEVKKAEKKAEVKEHKRRENERKRREWDEKLANLSSEEEKLKLI 123
           QP+SKR  KK++KQ+++     E++ E+++ KR+E +RKR++ +                
Sbjct: 38  QPISKRQMKKLMKQKQW-----EEQRELRKQKRKE-KRKRKQLE---------------- 97

Query: 124 ESKREMRKERMEQRSEERENKIQRLTQGKLHGQ-NIVIDLEFSNLMKPNELNSLVQQIMY 183
                 R+ + E  S+  + K  R+ +  +H    ++ID  F +LM   ++  L +QI  
Sbjct: 98  ------RQCQPESNSDGSDRK--RIRRDVVHSPLRLIIDCSFDSLMVLKDIKKLHKQIQR 157

Query: 184 CYAVNSRCTSPGHLWLTGCEGNMGDHLNRLPGFDK-WIIEKENRSYIEALQE--QKENLV 243
           CYA N R   P   +LT   G +  +++     DK W+  K+     E   E  QKE+L+
Sbjct: 158 CYAENRRALHPVQFYLTSHGGQLKKNMDE---NDKGWVNWKDIHIKPEHYSEFIQKEDLI 217

Query: 244 YLTADAETVLDDLDPKKIYIIGGLVDRNRWKGLTMKKAEEQGIKAAKLPIGNYLKMSSSQ 303
           YLT+D+  +L +LD  K Y+IGGLVD N  KGLT K+A + GI  A+LP+GN++KM+S +
Sbjct: 218 YLTSDSPNILKELDESKAYVIGGLVDHNHHKGLTYKQASDHGIDHAQLPLGNFVKMNSRK 277

Query: 304 VLTVNQVVEILLKYLEMKDWKASFFQVIPQRKRCDADSGDPEREIEDKEKTEDVSCTKNQ 363
           VL VN V EI+L+YLE +DW+ +FF ++PQRK            +   +  E  S  K  
Sbjct: 278 VLAVNHVFEIILEYLETRDWQEAFFTILPQRKGA----------VPTDQACESCSHDKKF 331

Query: 364 KKMK--LSETDSEGSEREVEIGEHEKSKSKPREDVES 395
            +++  L+   SE   R      HE+ K       ES
Sbjct: 338 ARVEGGLNSDSSEEENRHELDSTHEEEKQDKENSTES 331

		  

BLAST of Spo11585.1 vs. ExPASy Swiss-Prot
Match: TM10A_MOUSE (tRNA methyltransferase 10 homolog A OS=Mus musculus GN=Trmt10a PE=2 SV=2)

HSP 1 Score: 134.0 bits (336), Expect = 3.600e-30
Identity = 103/320 (32.19%), Postives = 171/320 (53.44%), Query Frame = 1

		  

Query: 63  SQPLSKRAQKKIIKQERYEVKKAEKKAEVKEHKRRENERKRREWDEKLANLSSEEEKLKL 122
           ++P+SKR  KK++KQ+++E ++ ++K + KE ++R+   +R+                  
Sbjct: 37  AEPISKRQLKKLMKQKQWEEQREQRKEKRKEKRKRKKLERRQ------------------ 96

Query: 123 IESKREMRKERMEQRSEERENKIQRLTQGKLHGQNIVIDLEFSNLMKPNELNSLVQQIMY 182
           +ES  +    +  +R   R +              +VID  F +LM   ++  L +QI  
Sbjct: 97  LESNSDGNDRKRVRRDVARSSL------------RLVIDCSFDDLMVLKDIKKLHKQIQR 156

Query: 183 CYAVNSRCTSPGHLWLTGCEGNMGDHLNRLP-GFDKWI-IEKENRSYIEALQEQKENLVY 242
           CYA N R + P   +LT   G +  +++    G+  W  I  ++  Y E ++  KE+LVY
Sbjct: 157 CYAENRRASHPVQFYLTSHGGQLKKNMDENDQGWVNWKDIHIKSEHYSELIK--KEDLVY 216

Query: 243 LTADAETVLDDLDPKKIYIIGGLVDRNRWKGLTMKKAEEQGIKAAKLPIGNYLKMSSSQV 302
           LT+D+  VL DLD  K Y+IGGLVD +  KGLT K+A   GI+ A+LP+ +++KM+S +V
Sbjct: 217 LTSDSPNVLKDLDESKAYVIGGLVDHSHHKGLTFKQATSYGIEHAQLPLADFVKMNSRKV 276

Query: 303 LTVNQVVEILLKYLEMKDWKASFFQVIPQR-------KRCDADSGDPEREIEDKEK-TED 362
           L VN V EI+L++LE +DW+ +FF ++PQR       K C++   D +   E  +  +E 
Sbjct: 277 LAVNHVFEIILEFLETRDWQEAFFTILPQRKGAVPAHKACESSPQDHQSLPEGWDSASEG 324

Query: 363 VSCTKNQKKMKLSETDSEGS 373
            SC  N    +  E   + S
Sbjct: 337 ESCRDNPDSPQKDEQGQQSS 324

		  

BLAST of Spo11585.1 vs. TAIR (Arabidopsis)
Match: AT5G47680.1 (FUNCTIONS IN: molecular_function unknown)

HSP 1 Score: 369.4 bits (947), Expect = 2.800e-102
Identity = 201/310 (64.84%), Postives = 249/310 (80.32%), Query Frame = 1

		  

Query: 59  PQPESQPLSKRAQKKIIKQERYEVKKAEKKAEVKEHKRRENERKRREWDEKLANLSSEEE 118
           P   S PLSK AQKK +KQ+RYE KKAEKKA+ KEHKR+E ERK +EW+E LAN ++EEE
Sbjct: 22  PVNVSPPLSKNAQKKQLKQQRYEAKKAEKKAQEKEHKRKEGERKLKEWEETLAN-ATEEE 81

Query: 119 KLKLIESKREMRKERMEQRSEERENKIQRLTQGKLHGQNIVIDLEFSNLMKPNELNSLVQ 178
           +LKLIES++ +RKERME+RSEE+E KI+RL Q K  GQ IV+D++F++LM  +E++SLVQ
Sbjct: 82  RLKLIESRKSLRKERMEKRSEEKEKKIERLNQAKEIGQKIVVDVDFAHLMSESEISSLVQ 141

Query: 179 QIMYCYAVNSRCTSPGHLWLTGCEGNMGDHLNRLPGFDKWIIEKENRSYIEALQEQKENL 238
           QIMYCYAVN R TSP HLWLTG +G M   L++LPGF+KW IEKE+R YIEA+ +QK+NL
Sbjct: 142 QIMYCYAVNGRSTSPCHLWLTGVQGKMSTELDKLPGFEKWFIEKESRCYIEAMADQKDNL 201

Query: 239 VYLTADAETVLDDLDPKKIYIIGGLVDRNRWKGLTMKKAEEQGIKAAKLPIGNYLKMSSS 298
           VYLTAD+ETVLDDLDPK IYIIGGLVDRNR+KG+TM KA+EQGIK AKLPIG Y+KMSSS
Sbjct: 202 VYLTADSETVLDDLDPKHIYIIGGLVDRNRFKGITMTKAQEQGIKTAKLPIGEYMKMSSS 261

Query: 299 QVLTVNQVVEILLKYLEMKDWKASFFQVIPQRKRCDADSGDPER--EIEDKEKTEDVSCT 358
           QVLTVNQV+EIL+K+LE +DWK +FF VIPQRKR   D  D  +   I ++ + +D    
Sbjct: 262 QVLTVNQVLEILVKFLETRDWKTAFFTVIPQRKRTGLDPVDCSKLEHISEEHQEKDTEAN 321

Query: 359 KNQKKMKLSE 367
           ++  K  L E
Sbjct: 322 ESADKHDLLE 330