Spo12689 (gene)

Overview
NameSpo12689
Typegene
OrganismSpinacia oleracea (Spinach)
DescriptionNAD-specific glutamate dehydrogenase (NAD-GDH) (1.4.1.2)
LocationSpoScf_00929 : 118303 .. 120045 (-)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAGGAGAGAGGAAGAGTAAACTAACCAAAAACGGTGTTAATGGGGTTCATGGCGACCTGATTCTTAGCGGCATCACCAATCAACCTTTCAGAATCGGTGAAAGCAACATAAGATGGAGTAGTTCTGTTACCCTGATCATTAGCAATGATCTCAACACGGTCGTGTTGCCATACTCCAACACAAGAGTAGGTGGTACCGAGATCAATACCGATAGCTGGACCTTCTCCTTTACCGGCCATTATAATCTTCCTAGGCAACGACGGAAGAAGAGAATGGGAGAAAAAAGAGGAGAGAGAATGTGAGAGAGAGTGTTTGAATGTGGTAGCAGTAGCAGCGGCTGAGAGAGAGAGGGAAAAAAATGTTAGGGTTTGGGATGGTTTTATAAGTGAAGGAGAAGCGCGGGCGGCTTATGTCATGTGGGAAAAAGGAAAAGGAAAATTTAAAAGGGTTTGTGTTTCTGTTTTTCTAGAATGTTCTTGTGGAGTCTCAGCTGTTGGATTTGATTTGGGGTTCATCGTGTGGTTGATGATGACCAATGGAATTTAGGTTATGAGTTGATCGGTTGAGTAAAGGCCAATTGCCAGAAGTATGTTAGTCAAAGTTTTGGGATGGTGGTGGGAAGGTGTGATTTAATTTTTTGAATATTGAGTGGTCCACGTATACAACTTATTTTGTTTACTACTCCTCCTGTCCCTTAATACTCGCACCATTTTGACTTTTTGCACTATTCACATAATTTACTTTGACCCTATTTTATTTCGGCAACTTTTCTGTAAGACCGCCTTATCAGAAAGACCACTTTGGTTGCTTAACTAATTGGTTCAATTTCTTAACTAATTGGTTACATTACTTAATTAATTGATTCAATTGCTTAACTAATTAATTCCTTTGCCTAACTAATTGGTTTCAGTGCTTAACTAATTAATTCAAGTGCTTAACTAATTGGTTCCATTGCTTAACTTATATGATACCAAAGTGGTCTTTTGTGTAAGACCGCCTTATAGAAAAGTTTGTAATTTTATTTCTAGTATATGAAAACAAATGTTAGTATATAATATATTATTGGCTTATCTTAATATATATTTTCATAATATTGAAAAAAAATTAAGTTTTTATAATATGAAGTTGAAGATATTCGTGGTCAAAGATGTGCATTGGCAAGCATGTCCAGTCAAAACGGTGCGAGTATTAAGGGACGGAGGGAGTACTCCGTATTATTTTTTTGTCGTCCCTACTAATTGTATTTCGGTTTTTCATGAAATGTCCATAATATTTAACGAAATGCACTAAATACTCTCAACTTTTTAAAAATATATTAAAAATCCTTATTGGCTAACGGATGTTAATCTAACTAGTCAAAGTAATCTTTAATTTTATTTTATTTTTTAAAAACAAAATTGAATTCATTAATAAAGAGTCATATATACGACATCTACCGAAGAAATATATGTCTAACAAATCCATGAAAAATTGAATAAAATAAAGGTATCCTTCACAAAACTATGGTCGTTGAATAAATCTTGCACTGTAGAACGTCTCTCGTATATAACGACGGAACATGCAGCCTTTTCAGAGAAAGGGTTTAACGATTAGTTGTAGCCGCGAGGATAATTTCCGGCCACTTGATTCATCTTAACTAGTTTGTGGACCCGTTGTTAGTGTGATGGAGTTTAGAAGTTATATTATTTGTAAGCAGGTCTTTAAACCAAATGACAATAATAAAACTTTACGACCTATGA

mRNA sequence

ATGAGAGGAGAGAGGAAGAGTAAACTAACCAAAAACGGTGTTAATGGGGTTCATGGCGACCTGATTCTTAGCGGCATCACCAATCAACCTTTCAGAATCGGTGAAAGCAACATAAGATGGAGTAGTTCTGTTACCCTGATCATTAGCAATGATCTCAACACGGTCGTGTTGCCATACTCCAACACAAGAGTAGGTGGTACCGAGATCAATACCGATAGCTGGACCTTCTCCTTTACCGGCCATTATAATCTTCCTAGGCAACGACGGAAGAAGAGAATGGGAGAAAAAAGAGGAGAGAGAATGTCTTTAAACCAAATGACAATAATAAAACTTTACGACCTATGA

Coding sequence (CDS)

ATGAGAGGAGAGAGGAAGAGTAAACTAACCAAAAACGGTGTTAATGGGGTTCATGGCGACCTGATTCTTAGCGGCATCACCAATCAACCTTTCAGAATCGGTGAAAGCAACATAAGATGGAGTAGTTCTGTTACCCTGATCATTAGCAATGATCTCAACACGGTCGTGTTGCCATACTCCAACACAAGAGTAGGTGGTACCGAGATCAATACCGATAGCTGGACCTTCTCCTTTACCGGCCATTATAATCTTCCTAGGCAACGACGGAAGAAGAGAATGGGAGAAAAAAGAGGAGAGAGAATGTCTTTAAACCAAATGACAATAATAAAACTTTACGACCTATGA

Protein sequence

MRGERKSKLTKNGVNGVHGDLILSGITNQPFRIGESNIRWSSSVTLIISNDLNTVVLPYSNTRVGGTEINTDSWTFSFTGHYNLPRQRRKKRMGEKRGERMSLNQMTIIKLYDL
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo12689.1Spo12689.1mRNA


Homology
[Close]
BLAST of Spo12689.1 vs. NCBI nr
Match: gi|1169326|sp|P41755.1|DHE2_ACHKL (RecName: Full=NAD-specific glutamate dehydrogenase; Short=NAD-GDH [Achlya klebsiana])

HSP 1 Score: 79.3 bits (194), Expect = 4.700e-12
Identity = 34/62 (54.84%), Postives = 49/62 (79.03%), Query Frame = 1 

		  

Query: 11   KNGVNGVHGDLILSGITNQPFRIGESNIRWSSSVTLIISNDLNTVVLPYSNTRVGGTEIN 70
            ++ V  VHG+L+LSGITN+ F + ESN+RW  +VTLI+ N+ NT+VLP ++TR+  TEI+
Sbjct: 974  EHSVGRVHGNLVLSGITNKTFAVSESNVRWGGTVTLIVGNNFNTIVLPDTDTRISRTEID 1033

Query: 71   TD 73
            TD
Sbjct: 1034 TD 1035
[Close]
BLAST of Spo12689.1 vs. NCBI nr
Match: gi|574141122|dbj|BAO38916.1| (putative uncharacterized protein YAL004W [Kluyveromyces marxianus DMKU3-1042])

HSP 1 Score: 76.6 bits (187), Expect = 3.000e-11
Identity = 35/63 (55.56%), Postives = 45/63 (71.43%), Query Frame = 1 

		  

Query: 11  KNGVNGVHGDLILSGITNQPFRIGESNIRWSSSVTLIISNDLNTVVLPYSNTRVGGTEIN 70
           +NGV+ VH  L+LS ITNQ F + E N RWS SVTL++  + NT+V    NT VGGT+IN
Sbjct: 160 ENGVSWVHSSLVLSSITNQSFGVSEGNERWSGSVTLVVGQNFNTIVRKVGNTGVGGTQIN 219

Query: 71  TDS 74
           T+S
Sbjct: 220 TNS 222
[Close]
BLAST of Spo12689.1 vs. NCBI nr
Match: gi|694428081|ref|XP_009341630.1| (PREDICTED: 7-deoxyloganetin glucosyltransferase-like [Pyrus x bretschneideri])

HSP 1 Score: 72.8 bits (177), Expect = 4.400e-10
Identity = 35/83 (42.17%), Postives = 55/83 (66.27%), Query Frame = 1 

		  

Query: 1   MRGERK----SKLTKNGVNGVHGDLILSGITNQPFRIGESNIRWSSSVTLIISNDLNTVV 60
           +R E+K    S LT++GV+G+HGDL+L  I +QP R+ + +IR    V LI  +DL+T+V
Sbjct: 253 IRSEQKESISSVLTEDGVDGIHGDLVLGRIADQPLRVSKRHIRRRRPVALIFGDDLDTIV 312

Query: 61  LPYSNTRVGGTEINTDSWTFSFT 80
           LP ++ R+  +EI+ D W  S +
Sbjct: 313 LPNTDARISRSEIDPDRWPLSLS 335
[Close]
BLAST of Spo12689.1 vs. NCBI nr
Match: gi|694448446|ref|XP_009350114.1| (PREDICTED: 7-deoxyloganetin glucosyltransferase-like [Pyrus x bretschneideri])

HSP 1 Score: 72.8 bits (177), Expect = 4.400e-10
Identity = 35/83 (42.17%), Postives = 55/83 (66.27%), Query Frame = 1 

		  

Query: 1   MRGERK----SKLTKNGVNGVHGDLILSGITNQPFRIGESNIRWSSSVTLIISNDLNTVV 60
           +R E+K    S LT++GV+G+HGDL+L  I +QP R+ + +IR    V LI  +DL+T+V
Sbjct: 485 IRSEQKESISSVLTEDGVDGIHGDLVLGRIADQPLRVSKRHIRRRRPVALIFGDDLDTIV 544

Query: 61  LPYSNTRVGGTEINTDSWTFSFT 80
           LP ++ R+  +EI+ D W  S +
Sbjct: 545 LPNTDARISRSEIDPDRWPLSLS 567
[Close]
BLAST of Spo12689.1 vs. NCBI nr
Match: gi|1009382770|gb|KYN05452.1| (NAD-specific glutamate dehydrogenase, partial [Cyphomyrmex costatus])

HSP 1 Score: 68.9 bits (167), Expect = 6.400e-9
Identity = 32/72 (44.44%), Postives = 48/72 (66.67%), Query Frame = 1 

		  

Query: 5   RKSKLTKNGVNGVHGDLILSGITNQPFRIGESNIRWSSSVTLIISNDLNTVVLPYSNTRV 64
           +K KLT+NGV GVH +LIL  IT+QP  +GE  + W   +TL+I +DLN  +L  ++ RV
Sbjct: 236 KKLKLTENGVAGVHSNLILRRITDQPLGVGEGYVAWRGPITLVIGDDLNFPMLEDTHARV 295

Query: 65  GGTEINTDSWTF 77
            G ++++D   F
Sbjct: 296 RGAQVDSDCRCF 307
[Close]
BLAST of Spo12689.1 vs. UniProtKB/TrEMBL
Match: W0T7Z6_KLUMA (Uncharacterized protein OS=Kluyveromyces marxianus DMKU3-1042 GN=KLMA_20458 PE=4 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 2.100e-11
Identity = 35/63 (55.56%), Postives = 45/63 (71.43%), Query Frame = 1 

		  

Query: 11  KNGVNGVHGDLILSGITNQPFRIGESNIRWSSSVTLIISNDLNTVVLPYSNTRVGGTEIN 70
           +NGV+ VH  L+LS ITNQ F + E N RWS SVTL++  + NT+V    NT VGGT+IN
Sbjct: 160 ENGVSWVHSSLVLSSITNQSFGVSEGNERWSGSVTLVVGQNFNTIVRKVGNTGVGGTQIN 219

Query: 71  TDS 74
           T+S
Sbjct: 220 TNS 222
[Close]
BLAST of Spo12689.1 vs. UniProtKB/TrEMBL
Match: A0A0P6GPL8_9CRUS (Uncharacterized protein (Fragment) OS=Daphnia magna PE=4 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 6.200e-11
Identity = 32/59 (54.24%), Postives = 48/59 (81.36%), Query Frame = 1 

		  

Query: 14  VNGVHGDLILSGITNQPFRIGESNIRWSSSVTLIISNDLNTVVLPYSNTRVGGTEINTD 73
           V GVH DL+L GIT++   + E N+RW  +VTL++ ++L+TVVLP+++TRVGGT+I+TD
Sbjct: 441 VLGVHRDLVLGGITDETLAVREGNVRWGGTVTLVVRDNLDTVVLPHADTRVGGTKIDTD 499
[Close]
BLAST of Spo12689.1 vs. UniProtKB/TrEMBL
Match: A0A0P5MG84_9CRUS (Uncharacterized protein OS=Daphnia magna PE=4 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 1.800e-10
Identity = 31/59 (52.54%), Postives = 47/59 (79.66%), Query Frame = 1 

		  

Query: 14  VNGVHGDLILSGITNQPFRIGESNIRWSSSVTLIISNDLNTVVLPYSNTRVGGTEINTD 73
           V GVH DL+L GIT++   + E N+RW  +VTL++ ++ +TVVLP+++TRVGGT+I+TD
Sbjct: 615 VLGVHRDLVLGGITDETLAVREGNVRWGGTVTLVVRDNFDTVVLPHADTRVGGTKIDTD 673
[Close]
BLAST of Spo12689.1 vs. UniProtKB/TrEMBL
Match: A0A0P6HXX3_9CRUS (Uncharacterized protein OS=Daphnia magna PE=4 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 1.800e-10
Identity = 31/59 (52.54%), Postives = 47/59 (79.66%), Query Frame = 1 

		  

Query: 14  VNGVHGDLILSGITNQPFRIGESNIRWSSSVTLIISNDLNTVVLPYSNTRVGGTEINTD 73
           V GVH DL+L GIT++   + E N+RW  +VTL++ ++ +TVVLP+++TRVGGT+I+TD
Sbjct: 626 VLGVHRDLVLGGITDETLAVREGNVRWGGTVTLVVRDNFDTVVLPHADTRVGGTKIDTD 684
[Close]
BLAST of Spo12689.1 vs. UniProtKB/TrEMBL
Match: B5VMS2_YEAS6 (Uncharacterized protein OS=Saccharomyces cerevisiae (strain AWRI1631) GN=AWRI1631_120310 PE=4 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 7.600e-9
Identity = 30/57 (52.63%), Postives = 41/57 (71.93%), Query Frame = 1 

		  

Query: 11  KNGVNGVHGDLILSGITNQPFRIGESNIRWSSSVTLIISNDLNTVVLPYSNTRVGGT 68
           +N V+ VH  L+LS +TNQ F I E+N RWS SVTL++ N++NT++   SNT VG T
Sbjct: 159 ENSVSWVHSSLVLSSVTNQSFSISETNERWSGSVTLVVGNNVNTIIRKVSNTGVGST 215
[Close]
BLAST of Spo12689.1 vs. ExPASy Swiss-Prot
Match: DHE2_ACHKL (NAD-specific glutamate dehydrogenase OS=Achlya klebsiana PE=3 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 2.900e-14
Identity = 34/62 (54.84%), Postives = 49/62 (79.03%), Query Frame = 1 

		  

Query: 11   KNGVNGVHGDLILSGITNQPFRIGESNIRWSSSVTLIISNDLNTVVLPYSNTRVGGTEIN 70
            ++ V  VHG+L+LSGITN+ F + ESN+RW  +VTLI+ N+ NT+VLP ++TR+  TEI+
Sbjct: 974  EHSVGRVHGNLVLSGITNKTFAVSESNVRWGGTVTLIVGNNFNTIVLPDTDTRISRTEID 1033

Query: 71   TD 73
            TD
Sbjct: 1034 TD 1035
[Close]
BLAST of Spo12689.1 vs. ExPASy Swiss-Prot
Match: YAA4_YEAST (Putative uncharacterized protein YAL004W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=YAL004W PE=5 SV=2)

HSP 1 Score: 65.9 bits (159), Expect = 3.400e-10
Identity = 29/54 (53.70%), Postives = 40/54 (74.07%), Query Frame = 1 

		  

Query: 11  KNGVNGVHGDLILSGITNQPFRIGESNIRWSSSVTLIISNDLNTVVLPYSNTRV 65
           +NG+  +H  LILS ITNQ F + ES+ RWS SVTLI+ N+++T++   SNTRV
Sbjct: 159 ENGIRRIHSSLILSSITNQSFSVSESDKRWSGSVTLIVGNNVHTIISKVSNTRV 212
The following BLAST results are available for this feature:
BLAST of Spo12689.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|1169326|sp|P41755.1|DHE2_ACHKL4.7e-1254.8RecName: Full=NAD-specific glu... [more]
gi|574141122|dbj|BAO38916.1|3.0e-1155.5putative uncharacterized prote... [more]
gi|694428081|ref|XP_009341630.1|4.4e-1042.1PREDICTED: 7-deoxyloganetin gl... [more]
gi|694448446|ref|XP_009350114.1|4.4e-1042.1PREDICTED: 7-deoxyloganetin gl... [more]
gi|1009382770|gb|KYN05452.1|6.4e-944.4NAD-specific glutamate dehydro... [more]
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BLAST of Spo12689.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
W0T7Z6_KLUMA2.1e-1155.5Uncharacterized protein OS=Klu... [more]
A0A0P6GPL8_9CRUS6.2e-1154.2Uncharacterized protein (Fragm... [more]
A0A0P5MG84_9CRUS1.8e-1052.5Uncharacterized protein OS=Dap... [more]
A0A0P6HXX3_9CRUS1.8e-1052.5Uncharacterized protein OS=Dap... [more]
B5VMS2_YEAS67.6e-952.6Uncharacterized protein OS=Sac... [more]
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BLAST of Spo12689.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 2
Match NameE-valueIdentityDescription
DHE2_ACHKL2.9e-1454.8NAD-specific glutamate dehydro... [more]
YAA4_YEAST3.4e-1053.7Putative uncharacterized prote... [more]
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BLAST of Spo12689.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 0
Match NameE-valueIdentityDescription
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019651Glutamate dehydrogenase, NAD-specificPFAMPF10712NAD-GHcoord: 11..75
score: 2.6

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006807 nitrogen compound metabolic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006468 protein phosphorylation
biological_process GO:0009408 response to heat
biological_process GO:0006457 protein folding
biological_process GO:0015810 aspartate transmembrane transport
biological_process GO:0015804 neutral amino acid transport
biological_process GO:0055085 transmembrane transport
biological_process GO:0015827 tryptophan transport
biological_process GO:0006833 water transport
biological_process GO:0071918 urea transmembrane transport
biological_process GO:0006950 response to stress
cellular_component GO:0016020 membrane
cellular_component GO:0005774 vacuolar membrane
cellular_component GO:0031969 chloroplast membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0015293 symporter activity
molecular_function GO:0032440 2-alkenal reductase [NAD(P)] activity
molecular_function GO:0015175 neutral amino acid transmembrane transporter activity
molecular_function GO:0015172 acidic amino acid transmembrane transporter activity
molecular_function GO:0015204 urea transmembrane transporter activity
molecular_function GO:0015250 water channel activity
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0005515 protein binding
molecular_function GO:0005524 ATP binding
molecular_function GO:0031072 heat shock protein binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0051082 unfolded protein binding
molecular_function GO:0004352 glutamate dehydrogenase (NAD+) activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
RNA-Seq Expression