Spo13093 (gene) | SpinachBase

Overview


Name	Spo13093
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein
Location	SpoScf_01428 : 95223 .. 95870 (-)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAACTCGCACATACATCCTCCATCTTCTTCTTCTTCTTCTCTCTACTTCTCCTCCTTGGGTCAATCGCAACCGTCGAATCCACCACCTCACCCAATCCAAAAACCCCCCTTGATGACTTCATCAACAAAAGTTGCCAAGTGACCCTGTACCCAGACTTGTGTTACTCTTCCCTCTCCTCCTCCTACACCGGTGACCCGGATCTCGGGAAACTAACTTATGCTGCTATTAACATTAGCCATGCTGAAGCCATTCATGCAGCAGATTACGTCATGACATTGAGTAATAAAAAGACTTCCACCGGAGATGATGAGGCAATTTTGGATTGCGTAGAGCTGACGGAAGAAAGTGTACAGCAGGTTCGGAAGTCGCGGAATCAGATGGCGACGGTTCTGAGGAGTGGAAGTAGAGCTCAGAAGAAGGATGAGAGGGAGAAGAGGAAGAGGAGGTTTGAGATGAGTAATGTGCAGACGTGGATGAGTGCGGCAATAACTGACCAAAATACTTGTATAGATGGGTTTAGCGACGACAAGGGTGGAAAAAAGGACGACGAGGAGGAGGAGGTGACTATGAAGGTCAGGATGGTGGTGAAGTTTATTAGTAACGCTTTGGCGTTGGTTAATAGTTTTGTGGATGCTACCCTTTAG

mRNA sequence

ATGCAACTCGCACATACATCCTCCATCTTCTTCTTCTTCTTCTCTCTACTTCTCCTCCTTGGGTCAATCGCAACCGTCGAATCCACCACCTCACCCAATCCAAAAACCCCCCTTGATGACTTCATCAACAAAAGTTGCCAAGTGACCCTGTACCCAGACTTGTGTTACTCTTCCCTCTCCTCCTCCTACACCGGTGACCCGGATCTCGGGAAACTAACTTATGCTGCTATTAACATTAGCCATGCTGAAGCCATTCATGCAGCAGATTACGTCATGACATTGAGTAATAAAAAGACTTCCACCGGAGATGATGAGGCAATTTTGGATTGCGTAGAGCTGACGGAAGAAAGTGTACAGCAGGTTCGGAAGTCGCGGAATCAGATGGCGACGGTTCTGAGGAGTGGAAGTAGAGCTCAGAAGAAGGATGAGAGGGAGAAGAGGAAGAGGAGGTTTGAGATGAGTAATGTGCAGACGTGGATGAGTGCGGCAATAACTGACCAAAATACTTGTATAGATGGGTTTAGCGACGACAAGGGTGGAAAAAAGGACGACGAGGAGGAGGAGGTGACTATGAAGGTCAGGATGGTGGTGAAGTTTATTAGTAACGCTTTGGCGTTGGTTAATAGTTTTGTGGATGCTACCCTTTAG

Coding sequence (CDS)

ATGCAACTCGCACATACATCCTCCATCTTCTTCTTCTTCTTCTCTCTACTTCTCCTCCTTGGGTCAATCGCAACCGTCGAATCCACCACCTCACCCAATCCAAAAACCCCCCTTGATGACTTCATCAACAAAAGTTGCCAAGTGACCCTGTACCCAGACTTGTGTTACTCTTCCCTCTCCTCCTCCTACACCGGTGACCCGGATCTCGGGAAACTAACTTATGCTGCTATTAACATTAGCCATGCTGAAGCCATTCATGCAGCAGATTACGTCATGACATTGAGTAATAAAAAGACTTCCACCGGAGATGATGAGGCAATTTTGGATTGCGTAGAGCTGACGGAAGAAAGTGTACAGCAGGTTCGGAAGTCGCGGAATCAGATGGCGACGGTTCTGAGGAGTGGAAGTAGAGCTCAGAAGAAGGATGAGAGGGAGAAGAGGAAGAGGAGGTTTGAGATGAGTAATGTGCAGACGTGGATGAGTGCGGCAATAACTGACCAAAATACTTGTATAGATGGGTTTAGCGACGACAAGGGTGGAAAAAAGGACGACGAGGAGGAGGAGGTGACTATGAAGGTCAGGATGGTGGTGAAGTTTATTAGTAACGCTTTGGCGTTGGTTAATAGTTTTGTGGATGCTACCCTTTAG

Protein sequence

MQLAHTSSIFFFFFSLLLLLGSIATVESTTSPNPKTPLDDFINKSCQVTLYPDLCYSSLSSSYTGDPDLGKLTYAAINISHAEAIHAADYVMTLSNKKTSTGDDEAILDCVELTEESVQQVRKSRNQMATVLRSGSRAQKKDEREKRKRRFEMSNVQTWMSAAITDQNTCIDGFSDDKGGKKDDEEEEVTMKVRMVVKFISNALALVNSFVDATL

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo13093.1	Spo13093.1	mRNA

Homology

[Close]

BLAST of Spo13093.1 vs. NCBI nr
Match: gi|902201102|gb|KNA13906.1| (hypothetical protein SOVF_112410 [Spinacia oleracea])

HSP 1 Score: 409.8 bits (1052), Expect = 2.900e-111
Identity = 215/215 (100.00%), Postives = 215/215 (100.00%), Query Frame = 1


		  
Query: 1   MQLAHTSSIFFFFFSLLLLLGSIATVESTTSPNPKTPLDDFINKSCQVTLYPDLCYSSLS 60
           MQLAHTSSIFFFFFSLLLLLGSIATVESTTSPNPKTPLDDFINKSCQVTLYPDLCYSSLS
Sbjct: 1   MQLAHTSSIFFFFFSLLLLLGSIATVESTTSPNPKTPLDDFINKSCQVTLYPDLCYSSLS 60

Query: 61  SSYTGDPDLGKLTYAAINISHAEAIHAADYVMTLSNKKTSTGDDEAILDCVELTEESVQQ 120
           SSYTGDPDLGKLTYAAINISHAEAIHAADYVMTLSNKKTSTGDDEAILDCVELTEESVQQ
Sbjct: 61  SSYTGDPDLGKLTYAAINISHAEAIHAADYVMTLSNKKTSTGDDEAILDCVELTEESVQQ 120

Query: 121 VRKSRNQMATVLRSGSRAQKKDEREKRKRRFEMSNVQTWMSAAITDQNTCIDGFSDDKGG 180
           VRKSRNQMATVLRSGSRAQKKDEREKRKRRFEMSNVQTWMSAAITDQNTCIDGFSDDKGG
Sbjct: 121 VRKSRNQMATVLRSGSRAQKKDEREKRKRRFEMSNVQTWMSAAITDQNTCIDGFSDDKGG 180

Query: 181 KKDDEEEEVTMKVRMVVKFISNALALVNSFVDATL 216
           KKDDEEEEVTMKVRMVVKFISNALALVNSFVDATL
Sbjct: 181 KKDDEEEEVTMKVRMVVKFISNALALVNSFVDATL 215

[Close]

BLAST of Spo13093.1 vs. NCBI nr
Match: gi|731339892|ref|XP_010681102.1| (PREDICTED: 21 kDa protein-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 196.8 bits (499), Expect = 3.800e-47
Identity = 124/224 (55.36%), Postives = 155/224 (69.20%), Query Frame = 1


		  
Query: 1   MQLAHTSSIFFFFFSLLLLLGSIATVESTTSPNPKTPLDDFINKSCQVTLYPDLCYSSLS 60
           MQ  H S +F F FSLLL        +S T+      LD+FI KSCQV+ Y +LCYSSLS
Sbjct: 1   MQFTHYSFLFLFLFSLLL--------QSITAFQSSKTLDEFIKKSCQVSRYSELCYSSLS 60

Query: 61  SSYTGDPDLGKLTYAAINISHAEAIHAADYV-MTLSNKKTST------GDDE--AILDCV 120
           SSYTGDPDLGKL+YAAI IS  +A+  A YV  T ++ KTST      G +E  ++ DC+
Sbjct: 61  SSYTGDPDLGKLSYAAIRISLDQALDTAHYVDETSTSSKTSTSSSSSSGAEELVSVRDCM 120

Query: 121 ELTEESVQQVRKSRNQMATVLRSGSRAQKKDERE-KRKRRFEMSNVQTWMSAAITDQNTC 180
           EL++++V QV+KS +QMA VLR       +DE E  RKRRFEMSNVQTWMSAAITDQ+TC
Sbjct: 121 ELSKDAVHQVKKSLDQMARVLR-------EDEVENSRKRRFEMSNVQTWMSAAITDQDTC 180

Query: 181 IDGFSDDKGGKKDDEEEEVTMKVRMVVKFISNALALVNSFVDAT 215
           +DGFS   GGK    + ++T KV M++KFISNALALVN FV ++
Sbjct: 181 VDGFSQVHGGKL---QNQLTAKVEMLLKFISNALALVNYFVQSS 206

[Close]

BLAST of Spo13093.1 vs. NCBI nr
Match: gi|922523861|ref|XP_013595744.1| (PREDICTED: 21 kDa protein-like [Brassica oleracea var. oleracea])

HSP 1 Score: 118.6 bits (296), Expect = 1.300e-23
Identity = 90/219 (41.10%), Postives = 120/219 (54.79%), Query Frame = 1


		  
Query: 5   HTSSIFFFFFSLLLLLGSIATVESTTSPNPKTPLDDFINKSCQVTLYPDLCYSSLSS-SY 64
           H S IFFFF + LL L  I TV S  S  P     DFI  SC  TLYPDLC SSLS+ S 
Sbjct: 20  HPSLIFFFFLATLLPL--ILTVHSQPSSLPSND-SDFIRMSCNTTLYPDLCISSLSNFSS 79

Query: 65  TGDPDLGKLTYAAINISHAEAIHAADYVMT----LSNKKTSTGDDEAIL---DCVELTEE 124
           +   D   L  AAI+++ +EA+    Y+      L +++ + G   A     DC E  ++
Sbjct: 80  SVHNDSALLARAAISVTLSEALELGSYLSNVTALLKSQEENGGHPTAAAVFHDCFENLKD 139

Query: 125 SVQQVRKSRNQMATVLRSGSRAQKKDEREKRKRRFEMSNVQTWMSAAITDQNTCIDGFSD 184
           +V ++R S  QM  ++ +GS             RF+MSNVQTW+SAA+TD+ TC DGF D
Sbjct: 140 AVDEMRGSMKQMRDLVSTGSL---------ESFRFQMSNVQTWLSAALTDEETCTDGFKD 199

Query: 185 DKGGKKDDEEEEVTMKVRMVVKFISNALALVNSFVDATL 216
               K +  ++EV  +V  V K  SNALALVN  VD  +
Sbjct: 200 ---VKDEPRKDEVCARVDDVKKLTSNALALVNRCVDKAM 223

[Close]

BLAST of Spo13093.1 vs. NCBI nr
Match: gi|565497160|ref|XP_006306219.1| (hypothetical protein CARUB_v10011899mg [Capsella rubella])

HSP 1 Score: 117.9 bits (294), Expect = 2.200e-23
Identity = 84/223 (37.67%), Postives = 121/223 (54.26%), Query Frame = 1


		  
Query: 5   HTSSIFFFFFSLLLLLGSIATVESTTSPNPKTPLDDFINKSCQVTLYPDLCYSSL---SS 64
           H   +FFFFF L+ +L  I TV S +  +      DFI  SC  TLYPD+C+SSL   SS
Sbjct: 6   HQPLLFFFFFFLVTVLPLILTVHSQSDDS------DFIRTSCNTTLYPDVCFSSLSSFSS 65

Query: 65  SYTGDPDLGKLTYAAINISHAEAIHAADYVMTLSNKKTSTGDD---------EAILDCVE 124
           S   DP L  L  AAI+++  +++  A Y+  ++  +  + DD             DC +
Sbjct: 66  SVHNDPAL--LARAAISVTLTKSVDLASYLANITIPQPESQDDGGGHHPTAAAVFHDCFD 125

Query: 125 LTEESVQQVRKSRNQMATVLRSGSRAQKKDEREKRKRRFEMSNVQTWMSAAITDQNTCID 184
             +++V+++R S  QM  ++ +GS             RF+MSNVQTW+SAA+TD+ TC D
Sbjct: 126 NVKDAVEEMRGSMKQMRELVSTGS---------LESFRFQMSNVQTWLSAALTDEETCTD 185

Query: 185 GFSDDKGGKKDDEEEEVTMKVRMVVKFISNALALVNSFVDATL 216
           GF D      +  ++EV  +V  V K  SNALALVN  VD  +
Sbjct: 186 GFKDI---HDEPRKDEVCARVDDVKKMTSNALALVNRCVDKAI 208

[Close]

BLAST of Spo13093.1 vs. NCBI nr
Match: gi|976911016|gb|KVH97678.1| (Pectinesterase inhibitor [Cynara cardunculus var. scolymus])

HSP 1 Score: 117.5 bits (293), Expect = 2.900e-23
Identity = 81/197 (41.12%), Postives = 107/197 (54.31%), Query Frame = 1


		  
Query: 24  ATVESTTSPNPKTPLD-DFINKSCQVTLYPDLCYSSLSS-SYTGDPDLGKLTYAAINISH 83
           A   +  +P+P T  D DFI  SC+ TLYP LCY+SLSS S     D G+L   AI ++ 
Sbjct: 11  AAAVAVANPSPPTSGDTDFIRTSCRTTLYPQLCYTSLSSYSNAVQQDPGRLARVAIGVTL 70

Query: 84  AEAIHAADYVMTLSNKKTSTGDDE---AILDCVELTEESVQQVRKSRNQMATVLRSGSRA 143
           ++A H A YV  +S      G      A+ DC  +  ++V ++R S  QM  +  SG   
Sbjct: 71  SKATHMAKYVSNISRNADYDGSPRVAAAVHDCFSVFGDAVDEIRGSLRQMRRLGGSGESL 130

Query: 144 QKKDEREKRKRRFEMSNVQTWMSAAITDQNTCIDGFSD--DKGGKKDDEEEEVTMKVRMV 203
                      RF++SNVQTWMSAA+T++ TC DGF D  D GG K     EV  +   V
Sbjct: 131 -----------RFQLSNVQTWMSAALTNEETCTDGFEDVADDGGVK----AEVCDRAVKV 190

Query: 204 VKFISNALALVNSFVDA 214
            +  SNALALVNSF +A
Sbjct: 191 KEVTSNALALVNSFANA 192

[Close]

BLAST of Spo13093.1 vs. UniProtKB/TrEMBL
Match: A0A0K9R309_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_112410 PE=4 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 2.000e-111
Identity = 215/215 (100.00%), Postives = 215/215 (100.00%), Query Frame = 1


		  
Query: 1   MQLAHTSSIFFFFFSLLLLLGSIATVESTTSPNPKTPLDDFINKSCQVTLYPDLCYSSLS 60
           MQLAHTSSIFFFFFSLLLLLGSIATVESTTSPNPKTPLDDFINKSCQVTLYPDLCYSSLS
Sbjct: 1   MQLAHTSSIFFFFFSLLLLLGSIATVESTTSPNPKTPLDDFINKSCQVTLYPDLCYSSLS 60

Query: 61  SSYTGDPDLGKLTYAAINISHAEAIHAADYVMTLSNKKTSTGDDEAILDCVELTEESVQQ 120
           SSYTGDPDLGKLTYAAINISHAEAIHAADYVMTLSNKKTSTGDDEAILDCVELTEESVQQ
Sbjct: 61  SSYTGDPDLGKLTYAAINISHAEAIHAADYVMTLSNKKTSTGDDEAILDCVELTEESVQQ 120

Query: 121 VRKSRNQMATVLRSGSRAQKKDEREKRKRRFEMSNVQTWMSAAITDQNTCIDGFSDDKGG 180
           VRKSRNQMATVLRSGSRAQKKDEREKRKRRFEMSNVQTWMSAAITDQNTCIDGFSDDKGG
Sbjct: 121 VRKSRNQMATVLRSGSRAQKKDEREKRKRRFEMSNVQTWMSAAITDQNTCIDGFSDDKGG 180

Query: 181 KKDDEEEEVTMKVRMVVKFISNALALVNSFVDATL 216
           KKDDEEEEVTMKVRMVVKFISNALALVNSFVDATL
Sbjct: 181 KKDDEEEEVTMKVRMVVKFISNALALVNSFVDATL 215

[Close]

BLAST of Spo13093.1 vs. UniProtKB/TrEMBL
Match: A0A0J8C9J4_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_6g139440 PE=4 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 2.700e-47
Identity = 124/224 (55.36%), Postives = 155/224 (69.20%), Query Frame = 1


		  
Query: 1   MQLAHTSSIFFFFFSLLLLLGSIATVESTTSPNPKTPLDDFINKSCQVTLYPDLCYSSLS 60
           MQ  H S +F F FSLLL        +S T+      LD+FI KSCQV+ Y +LCYSSLS
Sbjct: 1   MQFTHYSFLFLFLFSLLL--------QSITAFQSSKTLDEFIKKSCQVSRYSELCYSSLS 60

Query: 61  SSYTGDPDLGKLTYAAINISHAEAIHAADYV-MTLSNKKTST------GDDE--AILDCV 120
           SSYTGDPDLGKL+YAAI IS  +A+  A YV  T ++ KTST      G +E  ++ DC+
Sbjct: 61  SSYTGDPDLGKLSYAAIRISLDQALDTAHYVDETSTSSKTSTSSSSSSGAEELVSVRDCM 120

Query: 121 ELTEESVQQVRKSRNQMATVLRSGSRAQKKDERE-KRKRRFEMSNVQTWMSAAITDQNTC 180
           EL++++V QV+KS +QMA VLR       +DE E  RKRRFEMSNVQTWMSAAITDQ+TC
Sbjct: 121 ELSKDAVHQVKKSLDQMARVLR-------EDEVENSRKRRFEMSNVQTWMSAAITDQDTC 180

Query: 181 IDGFSDDKGGKKDDEEEEVTMKVRMVVKFISNALALVNSFVDAT 215
           +DGFS   GGK    + ++T KV M++KFISNALALVN FV ++
Sbjct: 181 VDGFSQVHGGKL---QNQLTAKVEMLLKFISNALALVNYFVQSS 206

[Close]

BLAST of Spo13093.1 vs. UniProtKB/TrEMBL
Match: A0A0D3D754_BRAOL (Uncharacterized protein OS=Brassica oleracea var. oleracea PE=4 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 9.200e-24
Identity = 90/219 (41.10%), Postives = 120/219 (54.79%), Query Frame = 1


		  
Query: 5   HTSSIFFFFFSLLLLLGSIATVESTTSPNPKTPLDDFINKSCQVTLYPDLCYSSLSS-SY 64
           H S IFFFF + LL L  I TV S  S  P     DFI  SC  TLYPDLC SSLS+ S 
Sbjct: 20  HPSLIFFFFLATLLPL--ILTVHSQPSSLPSND-SDFIRMSCNTTLYPDLCISSLSNFSS 79

Query: 65  TGDPDLGKLTYAAINISHAEAIHAADYVMT----LSNKKTSTGDDEAIL---DCVELTEE 124
           +   D   L  AAI+++ +EA+    Y+      L +++ + G   A     DC E  ++
Sbjct: 80  SVHNDSALLARAAISVTLSEALELGSYLSNVTALLKSQEENGGHPTAAAVFHDCFENLKD 139

Query: 125 SVQQVRKSRNQMATVLRSGSRAQKKDEREKRKRRFEMSNVQTWMSAAITDQNTCIDGFSD 184
           +V ++R S  QM  ++ +GS             RF+MSNVQTW+SAA+TD+ TC DGF D
Sbjct: 140 AVDEMRGSMKQMRDLVSTGSL---------ESFRFQMSNVQTWLSAALTDEETCTDGFKD 199

Query: 185 DKGGKKDDEEEEVTMKVRMVVKFISNALALVNSFVDATL 216
               K +  ++EV  +V  V K  SNALALVN  VD  +
Sbjct: 200 ---VKDEPRKDEVCARVDDVKKLTSNALALVNRCVDKAM 223

[Close]

BLAST of Spo13093.1 vs. UniProtKB/TrEMBL
Match: R0GT54_9BRAS (Uncharacterized protein OS=Capsella rubella GN=CARUB_v10011899mg PE=4 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 1.600e-23
Identity = 84/223 (37.67%), Postives = 121/223 (54.26%), Query Frame = 1


		  
Query: 5   HTSSIFFFFFSLLLLLGSIATVESTTSPNPKTPLDDFINKSCQVTLYPDLCYSSL---SS 64
           H   +FFFFF L+ +L  I TV S +  +      DFI  SC  TLYPD+C+SSL   SS
Sbjct: 6   HQPLLFFFFFFLVTVLPLILTVHSQSDDS------DFIRTSCNTTLYPDVCFSSLSSFSS 65

Query: 65  SYTGDPDLGKLTYAAINISHAEAIHAADYVMTLSNKKTSTGDD---------EAILDCVE 124
           S   DP L  L  AAI+++  +++  A Y+  ++  +  + DD             DC +
Sbjct: 66  SVHNDPAL--LARAAISVTLTKSVDLASYLANITIPQPESQDDGGGHHPTAAAVFHDCFD 125

Query: 125 LTEESVQQVRKSRNQMATVLRSGSRAQKKDEREKRKRRFEMSNVQTWMSAAITDQNTCID 184
             +++V+++R S  QM  ++ +GS             RF+MSNVQTW+SAA+TD+ TC D
Sbjct: 126 NVKDAVEEMRGSMKQMRELVSTGS---------LESFRFQMSNVQTWLSAALTDEETCTD 185

Query: 185 GFSDDKGGKKDDEEEEVTMKVRMVVKFISNALALVNSFVDATL 216
           GF D      +  ++EV  +V  V K  SNALALVN  VD  +
Sbjct: 186 GFKDI---HDEPRKDEVCARVDDVKKMTSNALALVNRCVDKAI 208

[Close]

BLAST of Spo13093.1 vs. UniProtKB/TrEMBL
Match: A0A103XVK9_CYNCS (Pectinesterase inhibitor OS=Cynara cardunculus var. scolymus GN=Ccrd_000218 PE=4 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 2.000e-23
Identity = 81/197 (41.12%), Postives = 107/197 (54.31%), Query Frame = 1


		  
Query: 24  ATVESTTSPNPKTPLD-DFINKSCQVTLYPDLCYSSLSS-SYTGDPDLGKLTYAAINISH 83
           A   +  +P+P T  D DFI  SC+ TLYP LCY+SLSS S     D G+L   AI ++ 
Sbjct: 11  AAAVAVANPSPPTSGDTDFIRTSCRTTLYPQLCYTSLSSYSNAVQQDPGRLARVAIGVTL 70

Query: 84  AEAIHAADYVMTLSNKKTSTGDDE---AILDCVELTEESVQQVRKSRNQMATVLRSGSRA 143
           ++A H A YV  +S      G      A+ DC  +  ++V ++R S  QM  +  SG   
Sbjct: 71  SKATHMAKYVSNISRNADYDGSPRVAAAVHDCFSVFGDAVDEIRGSLRQMRRLGGSGESL 130

Query: 144 QKKDEREKRKRRFEMSNVQTWMSAAITDQNTCIDGFSD--DKGGKKDDEEEEVTMKVRMV 203
                      RF++SNVQTWMSAA+T++ TC DGF D  D GG K     EV  +   V
Sbjct: 131 -----------RFQLSNVQTWMSAALTNEETCTDGFEDVADDGGVK----AEVCDRAVKV 190

Query: 204 VKFISNALALVNSFVDA 214
            +  SNALALVNSF +A
Sbjct: 191 KEVTSNALALVNSFANA 192

[Close]

BLAST of Spo13093.1 vs. ExPASy Swiss-Prot
Match: 21KD_DAUCA (21 kDa protein OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.100e-14
Identity = 68/199 (34.17%), Postives = 103/199 (51.76%), Query Frame = 1


		  
Query: 14  FSLLLLLGSIATVESTTSPNPKTPLDDFINKSCQVTLYPDLCYSSLSS-SYTGDPDLGKL 73
           FS LL++ + A+           P + FI  SC +T YP +C  SLS+ + T   +  +L
Sbjct: 10  FSALLVILAAASA---------APANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQEL 69

Query: 74  TYAAINISHAEAIHAADYVMTLSN-KKTSTGDDEAILDCVELTEESVQQVRKSRNQMATV 133
              A+ +S      A  ++  L+  K        AI DC+E  E+S+ +V +S ++M  +
Sbjct: 70  ASTALQVSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNL 129

Query: 134 LRSGSRAQKKDEREKRKRRFEMSNVQTWMSAAITDQNTCIDGFSDDKGGKKDDEEEEVTM 193
               S A+  D        F MSNV+TW+SAA+TD+ TC+DGF+    G     +E V  
Sbjct: 130 ----SHAKGNDFT------FRMSNVETWVSAALTDETTCMDGFAGK--GMDGKIKESVRA 187

Query: 194 KVRMVVKFISNALALVNSF 211
           +V  V +  SNALALVN+F
Sbjct: 190 QVVAVARVTSNALALVNNF 187

[Close]

BLAST of Spo13093.1 vs. ExPASy Swiss-Prot
Match: PME1_ARATH (Pectinesterase 1 OS=Arabidopsis thaliana GN=PME1 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 6.400e-10
Identity = 55/211 (26.07%), Postives = 97/211 (45.97%), Query Frame = 1


		  
Query: 17  LLLLGSIATV------ESTTSPNPKTPLDDFINKSCQVTLYPDLCYSSLS---SSYTGDP 76
           +++   +ATV      EST SP P+      +   C VT +P+ C SS+S   SS T DP
Sbjct: 42  VIIAAVVATVVHKNKNESTPSPPPELTPSTSLKAICSVTRFPESCISSISKLPSSNTTDP 101

Query: 77  D-LGKLTYAAINISHAEAIHAADYVMTLSNKKTSTGDDEAILDCVELTEESVQQVRKSRN 136
           + L KL+   I       I   D +  L  K +   +DE I   + +  + ++      N
Sbjct: 102 ETLFKLSLKVI-------IDELDSISDLPEKLSKETEDERIKSALRVCGDLIEDALDRLN 161

Query: 137 QMATVLRSGSRAQKKDEREKRKRRFEMSNVQTWMSAAITDQNTCIDGFSDDKGGKKDDEE 196
              + +         +E++K     ++ +++TW+SA +TD  TC D   + K  K +   
Sbjct: 162 DTVSAIDD-------EEKKKTLSSSKIEDLKTWLSATVTDHETCFDSLDELKQNKTEYAN 221

Query: 197 EEVTMKVRMVV----KFISNALALVNSFVDA 214
             +T  ++  +    +F SN+LA+V+  + A
Sbjct: 222 STITQNLKSAMSRSTEFTSNSLAIVSKILSA 238

[Close]

BLAST of Spo13093.1 vs. ExPASy Swiss-Prot
Match: PME1_CITSI (Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 7.000e-9
Identity = 59/220 (26.82%), Postives = 101/220 (45.91%), Query Frame = 1


		  
Query: 9   IFFFFFSLLLL----LGSIATVES--TTSPNPKTPLDDFINKSCQVTLYPDLCYSSL--- 68
           +F   F+ LL+    +G +A V S   +  N   P    +  SC  T YPDLC+S++   
Sbjct: 30  LFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPDLCFSAIAAV 89

Query: 69  ---SSSYTGDPDLGKLTYAAINISHAEAIHAADYVMTLSNKKTSTGDDEAIL-DCVELTE 128
              S   T   D+ +++   +NI+     H    +  L  +   T  ++  L DC+E  +
Sbjct: 90  PEASKKVTSQKDVIEMS---LNITTTAVEHNYFGIQKLLKRTNLTKREKVALHDCLETID 149

Query: 129 ESVQQVRKSRNQMATVLRSGSRAQKKDEREKRKRRFEMSNVQTWMSAAITDQNTCIDGFS 188
           E++ ++ K+   +       S +Q  D+            ++T MSAA+T+Q TC+DGFS
Sbjct: 150 ETLDELHKAVEDLEEYPNKKSLSQHADD------------LKTLMSAAMTNQGTCLDGFS 209

Query: 189 DDKGGKKDDEEEEVTMKVRMVVKFISNALALVNSFVDATL 216
            D   K     + ++     V K  SNALA++ +  D  +
Sbjct: 210 HDDANK--HVRDALSDGQVHVEKMCSNALAMIKNMTDTDM 232

[Close]

BLAST of Spo13093.1 vs. ExPASy Swiss-Prot
Match: PME3_CITSI (Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 7.000e-9
Identity = 59/220 (26.82%), Postives = 101/220 (45.91%), Query Frame = 1


		  
Query: 9   IFFFFFSLLLL----LGSIATVES--TTSPNPKTPLDDFINKSCQVTLYPDLCYSSL--- 68
           +F   F+ LL+    +G +A V S   +  N   P    +  SC  T YPDLC+S++   
Sbjct: 30  LFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPDLCFSAIAAV 89

Query: 69  ---SSSYTGDPDLGKLTYAAINISHAEAIHAADYVMTLSNKKTSTGDDEAIL-DCVELTE 128
              S   T   D+ +++   +NI+     H    +  L  +   T  ++  L DC+E  +
Sbjct: 90  PEASKKVTSQKDVIEMS---LNITTTAVEHNYFGIQKLLKRTNLTKREKVALHDCLETID 149

Query: 129 ESVQQVRKSRNQMATVLRSGSRAQKKDEREKRKRRFEMSNVQTWMSAAITDQNTCIDGFS 188
           E++ ++ K+   +       S +Q  D+            ++T MSAA+T+Q TC+DGFS
Sbjct: 150 ETLDELHKAVEDLEEYPNKKSLSQHADD------------LKTLMSAAMTNQGTCLDGFS 209

Query: 189 DDKGGKKDDEEEEVTMKVRMVVKFISNALALVNSFVDATL 216
            D   K     + ++     V K  SNALA++ +  D  +
Sbjct: 210 HDDANK--HVRDALSDGQVHVEKMCSNALAMIKNMTDTDM 232

[Close]

BLAST of Spo13093.1 vs. ExPASy Swiss-Prot
Match: PME34_ARATH (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana GN=PME34 PE=2 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 1.000e-7
Identity = 58/209 (27.75%), Postives = 98/209 (46.89%), Query Frame = 1


		  
Query: 15  SLLLLLGSIATVESTTSPNPKTP-----LDDFINKSCQVTLYPDLCYSSL-----SSSYT 74
           SL+L     A V S    N   P         I+K+C++T +P+LC  SL     S + +
Sbjct: 55  SLILAAAIFAGVRSRLKLNQSVPGLARKPSQAISKACELTRFPELCVDSLMDFPGSLAAS 114

Query: 75  GDPDLGKLTYAAINISHAEAIHAADYVMTLSNKKTSTGDDEAILDCVELTEESVQQVRKS 134
              DL  +T   +N++     HA     +LS          A   CVEL ++SV  + ++
Sbjct: 115 SSKDLIHVT---VNMTLHHFSHALYSSASLSFVDMPPRARSAYDSCVELLDDSVDALSRA 174

Query: 135 RNQMATVLRSGSRAQKKDEREKRKRRFEMSNVQTWMSAAITDQNTCIDGFSD-DKGGKKD 194
              +++V+ S ++ Q               +V TW+SAA+T+ +TC +GF   D GG KD
Sbjct: 175 ---LSSVVSSSAKPQ---------------DVTTWLSAALTNHDTCTEGFDGVDDGGVKD 234

Query: 195 DEEEEVTMKVRMVVKFISNALALVNSFVD 213
                +T  ++ + + +SN LA+ ++  D
Sbjct: 235 ----HMTAALQNLSELVSNCLAIFSASHD 238

[Close]

BLAST of Spo13093.1 vs. TAIR (Arabidopsis)
Match: AT2G01610.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 115.5 bits (288), Expect = 3.900e-26
Identity = 86/219 (39.27%), Postives = 121/219 (55.25%), Query Frame = 1


		  
Query: 4   AHTSSIFFFFFSLLLLLGSIATVESTTSPNPKTPLD-DFINKSCQVTLYPDLCYSSLSSS 63
           +HT++ F FF + LL+ GSI+ V     PN  T  D DFI  SC  TLYPD+C++SLS  
Sbjct: 7   SHTTT-FLFFTTFLLIFGSISAVRLLPRPNTTTTNDLDFIRTSCNATLYPDVCFTSLSGY 66

Query: 64  YTGDPDL-GKLTYAAINISHAEAIHAADYVMTLSNKKTSTGDD------EAILDCVELTE 123
            +   D   +L   AI +S ++A   A ++  LS        D        I DCV   E
Sbjct: 67  ASAVQDSPARLAKLAIGVSLSQAKSTAAFLSKLSRSAAKYSGDGHQTASAVIRDCVSNVE 126

Query: 124 ESVQQVRKSRNQMATVL-RSGSRAQKKDEREKRKRRFEMSNVQTWMSAAITDQNTCIDGF 183
           ++V ++R S  Q+  +  R G  A +   R     RF+MSNVQTWMSAA+TD++TC DGF
Sbjct: 127 DAVDEMRGSLRQLRDMNGRGGGTAAR---RSVETFRFQMSNVQTWMSAALTDEDTCTDGF 186

Query: 184 SD-DKGGKKDDEEEEVTMKVRMVVKFISNALALVNSFVD 213
            D D+GG     +  V  ++  V +  SNALALVN++ +
Sbjct: 187 EDMDEGGL---IKTTVCDRLEEVKRLTSNALALVNTYAN 218

[Close]

BLAST of Spo13093.1 vs. TAIR (Arabidopsis)
Match: AT1G23205.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 108.6 bits (270), Expect = 4.800e-24
Identity = 81/222 (36.49%), Postives = 119/222 (53.60%), Query Frame = 1


		  
Query: 5   HTSSIFFFFFSLLLLLGSIATVESTTSPNPKTPLDDFINKSCQVTLYPDLCYSSL---SS 64
           H   +FFF  S+L L   I TV S +  +      DFI  SC  TLYPDLC+SSL   SS
Sbjct: 6   HQPLLFFFLASVLPL---ILTVHSQSDDS------DFIRTSCNTTLYPDLCFSSLSSFSS 65

Query: 65  SYTGDPDLGKLTYAAINISHAEAIHAADYVMTLSNKKTSTGDDEA--------ILDCVEL 124
           S   DP L  L  AAI+++  + +  A Y+  ++  +  + +D A          DC + 
Sbjct: 66  SVHNDPAL--LARAAISVTLTKTLDLASYLANITTLQPESNEDGAHHPTAAAVFHDCFDN 125

Query: 125 TEESVQQVRKSRNQMATVLRSGSRAQKKDEREKRKRRFEMSNVQTWMSAAITDQNTCIDG 184
            +++V++++ S  QM  ++ +GS             RF+MSNVQTW+SAA+TD+ TC DG
Sbjct: 126 LKDAVEEMKGSMKQMRELVSTGS---------LESFRFQMSNVQTWLSAALTDEETCTDG 185

Query: 185 FSDDKGGKKDDEEEEVTMKVRMVVKFISNALALVNSFVDATL 216
           F D      +  ++++  +V  V K  SNALALVN  VD  +
Sbjct: 186 FKDI---HDEPRKDDICARVDDVKKMTSNALALVNRCVDKAI 204

[Close]

BLAST of Spo13093.1 vs. TAIR (Arabidopsis)
Match: AT1G70720.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 101.7 bits (252), Expect = 5.900e-22
Identity = 72/204 (35.29%), Postives = 108/204 (52.94%), Query Frame = 1


		  
Query: 15  SLLLLLGSIATVESTTSPNPKTPLD--DFINKSCQVTLYPDLCYSSLSSSYTG-DPDLGK 74
           +LL+LL S    +  T   P  P +  DFI  +C  TLYPDLC+S+LSS       D  +
Sbjct: 8   TLLILLFSTFLPQILTVDPPLLPSNGSDFIRLACNTTLYPDLCFSTLSSFANSIQNDSNR 67

Query: 75  LTYAAINISHAEAIHAADYVMTLSNKKTSTGDDEAILDCVELTEESVQQVRKSRNQMATV 134
           L   AI+++    +H   Y+    N+   T     + DC E  +++V  +R S  QM  +
Sbjct: 68  LARVAISLTLHNTLHLLSYLQNAYNRDHPT---PVLRDCFENLKDAVDGMRGSMKQMKEL 127

Query: 135 LRSGSRAQKKDEREKRKRRFEMSNVQTWMSAAITDQNTCIDGFSDDKGGKKDDEEEEVTM 194
           + +    +          RF+MSNV+TW+SAA+TD+ TC DGF D    + D  +++V  
Sbjct: 128 VSASGSIESF--------RFQMSNVKTWLSAALTDEYTCTDGFKDVH--EDDSIKDDVCS 187

Query: 195 KVRMVVKFISNALALVNSFVDATL 216
           +V  V K  SNALALVN + D ++
Sbjct: 188 RVDDVKKLTSNALALVNRYADESI 198

[Close]

BLAST of Spo13093.1 vs. TAIR (Arabidopsis)
Match: AT1G14890.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 97.4 bits (241), Expect = 1.100e-20
Identity = 74/200 (37.00%), Postives = 108/200 (54.00%), Query Frame = 1


		  
Query: 16  LLLLLGSIATVESTTSP--NPKTPLDDFINKSCQVTLYPDLCYSSLSSSYTGDPDL-GKL 75
           L+ LL    T+ +   P   P T   DFI  SC  TLYPD+CY+SL+   +   D   +L
Sbjct: 31  LVTLLFICRTISAVRFPPEQPTTDDLDFIRTSCNTTLYPDVCYTSLAGYASAVQDNPARL 90

Query: 76  TYAAINISHAEAIHAADYVMTLSNKKTSTGDDEAILDCVELTEESVQQVRKSRNQMATVL 135
              AI +S + A + A Y+  LS +  S     A+ DCV    ++V Q+R S  Q    L
Sbjct: 91  AKLAIGVSLSRAKYTAAYLSKLSRRAASA----AVHDCVSNVGDAVDQMRGSLRQ----L 150

Query: 136 RSGSRAQKKDEREKRKRRFEMSNVQTWMSAAITDQNTCIDGFSDDKGGKKDDEEEEVTMK 195
           R  +  +  D       RF+MSNVQTWMSAA+TD+ TC DG +++   +  + +  +  +
Sbjct: 151 REMNHRRPGDPAF----RFQMSNVQTWMSAALTDEETCTDGVTEEM--EDGETKTAICDR 210

Query: 196 VRMVVKFISNALALVNSFVD 213
           V  V +F SNALALVN++ +
Sbjct: 211 VADVKRFTSNALALVNTYAN 216

[Close]

BLAST of Spo13093.1 vs. TAIR (Arabidopsis)
Match: AT1G62770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 94.7 bits (234), Expect = 7.200e-20
Identity = 72/201 (35.82%), Postives = 110/201 (54.73%), Query Frame = 1


		  
Query: 17  LLLLGSIATVESTTSPNPKTPLDDFINKSCQVTLYPDLCYSSLSSSYT-----GDPDLGK 76
           L++L SI  ++S+++   ++  D FI  SCQ T YP LC  +LS+  T      D DL +
Sbjct: 9   LVILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLAQ 68

Query: 77  LTYAAINISHAEAIHAADYVMTLSNKKTSTGDDEAILDCVELTEESVQQVRKSRNQMATV 136
            T   I+++ A+++      +T    K    +  AI DC+E+   SV ++ +S  ++A  
Sbjct: 69  -TALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELA-- 128

Query: 137 LRSGSRAQKKDEREKRKRRFEMSNVQTWMSAAITDQNTCIDGFSDDKGGKKDDEEEEVTM 196
            R+G     +D   K      MSNVQTW+SAA+TD+ TC+DGFS+   G K   +  +  
Sbjct: 129 -RAGHAVASEDFMWK------MSNVQTWVSAALTDETTCLDGFSERAMGGK--VKRLIRY 188

Query: 197 KVRMVVKFISNALALVNSFVD 213
           KV  V +  SNALALVN F +
Sbjct: 189 KVVHVAQVTSNALALVNQFAE 197

The following BLAST results are available for this feature:

BLAST of Spo13093.1 vs. NCBI nr
BLAST of Spo13093.1 vs. UniProtKB/TrEMBL
BLAST of Spo13093.1 vs. ExPASy Swiss-Prot
BLAST of Spo13093.1 vs. TAIR (Arabidopsis)

BLAST of Spo13093.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902201102\|gb\|KNA13906.1\|	2.9e-111	100.	hypothetical protein SOVF_1124...	[more]
gi\|731339892\|ref\|XP_010681102.1\|	3.8e-47	55.3	PREDICTED: 21 kDa protein-like...	[more]
gi\|922523861\|ref\|XP_013595744.1\|	1.3e-23	41.1	PREDICTED: 21 kDa protein-like...	[more]
gi\|565497160\|ref\|XP_006306219.1\|	2.2e-23	37.6	hypothetical protein CARUB_v10...	[more]
gi\|976911016\|gb\|KVH97678.1\|	2.9e-23	41.1	Pectinesterase inhibitor [Cyna...	[more]

BLAST of Spo13093.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9R309_SPIOL	2.0e-111	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8C9J4_BETVU	2.7e-47	55.3	Uncharacterized protein OS=Bet...	[more]
A0A0D3D754_BRAOL	9.2e-24	41.1	Uncharacterized protein OS=Bra...	[more]
R0GT54_9BRAS	1.6e-23	37.6	Uncharacterized protein OS=Cap...	[more]
A0A103XVK9_CYNCS	2.0e-23	41.1	Pectinesterase inhibitor OS=Cy...	[more]

BLAST of Spo13093.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
21KD_DAUCA	1.1e-14	34.1	21 kDa protein OS=Daucus carot...	[more]
PME1_ARATH	6.4e-10	26.0	Pectinesterase 1 OS=Arabidopsi...	[more]
PME1_CITSI	7.0e-9	26.8	Pectinesterase 1 OS=Citrus sin...	[more]
PME3_CITSI	7.0e-9	26.8	Pectinesterase 3 OS=Citrus sin...	[more]
PME34_ARATH	1.0e-7	27.7	Probable pectinesterase/pectin...	[more]

BLAST of Spo13093.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT2G01610.1	3.9e-26	39.2	Plant invertase/pectin methyle...	[more]
AT1G23205.1	4.8e-24	36.4	Plant invertase/pectin methyle...	[more]
AT1G70720.1	5.9e-22	35.2	Plant invertase/pectin methyle...	[more]
AT1G14890.1	1.1e-20	37.0	Plant invertase/pectin methyle...	[more]
AT1G62770.1	7.2e-20	35.8	Plant invertase/pectin methyle...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	GENE3D	1.20.140.40		coord: 40..210 score: 2.4
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 40..206 score: 1.2
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 37..206 score: 2.5
IPR006501	Pectinesterase inhibitor domain	TIGRFAMs	TIGR01614	TIGR01614	coord: 14..208 score: 7.3
IPR006501	Pectinesterase inhibitor domain	unknown	SSF101148	Plant invertase/pectin methylesterase inhibitor	coord: 39..210 score: 6.02
None	No IPR available	PANTHER	PTHR31080	FAMILY NOT NAMED	coord: 1..212 score: 4.7
None	No IPR available	PANTHER	PTHR31080:SF10	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR DOMAIN-CONTAINING PROTEIN	coord: 1..212 score: 4.7

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0005985	sucrose metabolic process
biological_process	GO:0010075	regulation of meristem growth
biological_process	GO:0006979	response to oxidative stress
biological_process	GO:0042744	hydrogen peroxide catabolic process
biological_process	GO:0048653	anther development
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016020	membrane
cellular_component	GO:0009505	plant-type cell wall
cellular_component	GO:0009506	plasmodesma
cellular_component	GO:0005773	vacuole
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004601	peroxidase activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo07373	0.70	Barchart \| Table
Spo26052	0.67	Barchart \| Table