Spo15297 (gene)

Overview
NameSpo15297
Typegene
OrganismSpinacia oleracea (Spinach)
DescriptionAsparagine synthetase (6.3.5.4)
Locationchr3 : 48532962 .. 48537450 (-)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTGAGTGGAATCAGTTAAGGACCTTAGGAAAGGCGAGGGATTGTGAGTCTATGATTATCTCCAGTGATTTGTAAGCAGCCTTACATTTCGTCGTTGGTTGCTGCATATATTTTATATTTTTCTCTCCCTGAAATCCCAATCGCACTAGAGATCCATAACAAACATAAAAGATGTATTACAAAAGATTAAGTTGGCTTTTAGACAATTGGGGTAGAAATGGAGTTTCTTATAAATGAAGTAGTTTACCGTAACATGCTATCCTAGATTTTCTTTTGCGCTCAAGAGAGTCGAATTCAACTACTGTTTTTGGTTTAGTCATCGTTCTTTATTCATTTAATTGAAAAATGTGTGCTATGCATATATTACTCGGTAAATACGCTATCTTTGCAGGGTCCACTTGGTTTGCTTTAGAAATGAAAGCGATAAGTGATGATTGTGAACGGTTCATCTCTTTTCCTTCTGGACATATATATTCAAGCAAACAAGGTATGCTACTATCTAATTCATAAACTATCTGGCTAAGACATGGTTTTAACTTAAAACTAGGCATTTAAGGACTTAAATGCCATTAAAGTGAACTTATTGCTGGTGGATTGAGAAGGTGGAACAACCCGCCATGGTACTCAGAGCAGATACCTTCAATTCCATACGACCCAACAATTTTATGTGTAGCTTTTGAAAAGGTTTATTGAAATTGTTGATTGTCCATTTTCTCTTAGTTTCAGCGTCACAATAACCCAAACTTCTCCCTTGTCTTTACTACTCGGCCATTTCAACTTGCTCCTGACCTAAGAACTTGTTTATATGATATGAAGAATTTGTTCTCTTCTTATTTTTGCTGAATATGAAATTACTTGTGTTAGATTTCTTCTTATTTTCTGATTTACGGTAGACTTAAATCTTCTTGAAATTAATTTAACTTTTGATTTATTAACCTACTCTCTTGACTTCTTTCAGTATGTACTTAAGAGATTAATGACAGATGTCCCATTTGGTGTACTTCTGTCAGGTGGATTAGATTCATCACTTGTTGCTGCTGTAGCTTCACGTTACTTGGAAGAAATGGAAGGAGCTTACCAATGGGGTTCACAGTTGCACTCCTTTTGCATTGGACTGGAGGTGAATATGCCTTTGTACTCCAGATGTAAAAAAAATTCAGCAGTTGTTGCTTAAGTTAAACTGCTATGCTTATTTTGTTCATTTTTCCCCCTAAAAAACAAAAAACAAAAAACTAGGGGGTGTTTGTTTTGTGGGAGTTTGAAGGTGGGAATTGTAGTTTTAATCCCAAGTCCTCAAAGTCCCATGTTTTTTTGAAGGAAATAAAAATAAAGGGTTGGTGGGAATGGGCTTAAGGATATTTTCCTTCTATTCCCTACTTTTATCTAGGGGGTGGGGTGGGGTGAGGTGGTGGTGGGGGTATTAGCTATTAGAGGGACGGGAATAGGAAAAAAAAGGGACCTTTTCTCTTTAATGAAAGAGAATAGAATTGGGAAAAAATGGTTATTTTTCCCTGATGCTTCTCTTTGTTCCATTTTCTCAACATAACTATTCTTCTTGAGCTAGTTAGGTTTTGATTGTCGTGGGGGAGCCCAAAGAGTTGGATTTGATAATTGCTTGTTCTTCTGGATAGGAAATATGAAAGTTAACTTGTAACAGTACTTCCAGTATCTACGATGTGATGAATTTACATAAAACTACATCTTCTTAGACGAGTTGATAACAAAAATAAATATTGTCGGACTATAATAATCTAGACGAGAATTTAGTGCCAAAGTGGTGATAAGACCAGTAATGATAACATGATTTAGTTATAGAATCTCGGCAAAGGATACTATAACTAAATCCTGTTATCAAGGGGCAGTATTTTATAAACCTTTGGGGGATTAATCTCAAAATATAGTAGTTGTCGTTGATTTCTTTGCATCTTCCCTCTTCGCTACAATTTTCCCAACCTTCTACAATACTTAACCTTTCTATCTTCAGTTCTTTGTTCATTATTTCTGGTTTCACTTTTTATAGCTTAGAGAGGTAATTGTTAGTTTTATCATGATTCATATGTTTATGTTTGATTCAAGACGAGATTGGTAATGATTGTGCTTTTGGACTAGGGGCTAGCCTCGTCGTTGTTGGTGTAATTCTTGTTGTAAGCTTCTAGTTTCTTTGGATCAGACTTACTTAAATGAAGTAGATTCCATCTTGTAAAGAAAATCATGAATAACTTGTTTAAATGTATCCATACTAGGAGGTGATGGCTGTATTTTTTTGATAACTATCTCATTAGTTTACTTTTTGCGTTGGCACTTTGCATTATACATTGTTAGTTATATCATGACTCATGTTTTCTAATCAAAATCCAAACTGCTTGATCAGTTGAGATGATTTTGTTCTGCCAAGCTTATTCAACTCAACAACCTATCTAATCTCCTTCTTGCTTCTGGTTTTGGCTTCAATAAAGAATCTTGTCTAAATCACTAGCTGTCTTTCTAGGGCCTCTCTATGGGCGCTTTAAGCCTTTAACCCGTTCATATATATATCAATGCAACGACAACAAATCTGGTTAAATTTGAACAATGAGTATATTTCTACTATGTTGTAAATCTTGCTTGTTGATCTACTGATGTGAAATTTGAGCATATAATGAAACAAACTGTTTTACAAGGTTTCGATAGAATAAACCAAAAAGAGTAGTATAGCTACTGACTAGGAAAATCAGGTCTAGAGAACGCCAAACTGCCAAGTGGTTATGGTTGCTAAGCTAATCACTGCTAAAATTTTATTCTTCATATTGTTTGTTATTGGCTTGATGTTTCTTGGTTCAGTGCTACTGAAATGCCTCTCTGCTGATTTGTATTGCTGATGTACTTGGTGATGCTTCCTGAAATATTGGAATCTCAATTGTATAGCTAGTTGGAATCTCAATTGCTGATGTACTTGGTGATGCTTCCTGAAAATTACTGAAATGCCTCCCTGTTAACAAATCTGTCAATAGATTTATTAGGTCAGTTTTCAGACTTTGATATGTATTTTGATTCTAATATTTGTTTTTTTCACTCTTTAATCTGAAATAATTTTCGATACATGCTTATATGGATGTGAGAATTGGCTATTACTTATTGCTTGGCTGATTAGAGAGTACTTTTATCATGAAATTTTTCAAGATAATACTCAAGTTATATATTGATGTTAGAAATCTCGTGAATAACAGGTACCAAGTACTTATTGCATTTTTTAAACTTTAATTTTTTATACCAAGTCATGCTGTCATAATATAAACCCCACCACATATTCTTCCTCCTAATTTTATACTCCGTAATTTAGTTTGGCTAAGTTAATTGTGTTTACTTAATTGATCTGTGTTTCTTGTTTCTTGCGCTGCATTCTGATCTCTTCGTGTACTGTGTTTCTGTACTGTTGTGCTGCATCCTGATTTGATCATATTCTGCTTTTCTGATCTGTACTGATGCTTACTGATCTACATTGATGTTTATTGATCTGAACGATCGCTGCATACTGCTCTGCAAGGCTGCTGCATCATTAAGGTGCTTACGTATTGTTCTCGTCCCAACTTTGATCCTCATATTTCAGAACTAGGAGTTCAACAATGAAGATTCTGTATAGCTAAATACTTGTGCCAAAAATAAAATGAGAACTTTATTTGCTGATAGAGGGAGTAGTATATCGTACTTTAATAATATTCTTTTTGATGCAAAAACTAGTTGTTGTTGCATTCATTGATACTGCGTTGTTGTTGTTGTGTTGATGCAAGTACCTACGTTGTTGCTGCATCGATGCAGTCAGGTCTGGCAGAAGAGAGATTGCATAAATGCAGTAGCCTGTTGCTGTCCTTGTGCATTTTTTGTCATGAGGAGGCACAATATTCATTACCATGAATAAACATCTATAAATCTTGGCACATCCAATGAGGACTTCAAAAACCTAAATGCATAAAGCAATAAAAATGGTGGTGTTTGTTATTCAAGTATGCATTTTTTGGATTTCATGTGTGTCCGGGAGAGTTAAAACATATAATATCTTTTCCATACGTAAAGAATTGTTAAAGATCTTATATATCTATGTGATTTTATAAGTATCTTAGTCTCTTATGTGTTTCCTTTGTTTCCTGGTTGTGGCTCTTGGAAGTCCTCTTATACTATTCATGTTTCTTTTTCAGTGGATCATGTTCTTTATTTTTTGAAGAAAGAAAGCACGTCAAAGTAAGTAAGTTTTTTTCTCTTTAGACACCGATGGTAGGTTTTACTTTTTTCTCCAGTCAAATTCACCACCTTCGATATGAATGTTTGTCTTGCTCCACAATCTTCGTGAGTCTTTGCTAAAACAACTCAAATAGAACCTAGAGATGCAAAAATGACCATTGGTTGAGCTATCTTGTTTTTAGATAGGATGTTCTTACATTTTATTTCCCAAAGTCAACCTTGGTAATGCACAAAAGAAATTTAAAAGTCAAAACAAACACATACTAGCCAA

mRNA sequence

ATGATTGAGTGGAATCAGTTAAGGACCTTAGGAAAGGCGAGGGATTGGTCCACTTGGTTTGCTTTAGAAATGAAAGCGATAAGTGATGATTGTGAACGGTTCATCTCTTTTCCTTCTGGACATATATATTCAAGCAAACAAGGTGGATTAGATTCATCACTTGTTGCTGCTGTAGCTTCACGTTACTTGGAAGAAATGGAAGGAGCTTACCAATGGGGTTCACAGTTGCACTCCTTTTGCATTGGACTGGAGCTAGTTGGAATCTCAATTGCTGATGTACTTGGTGATGCTTCCTGAAAATTACTGAAATGCCTCCCTGTTAACAAATCTGTCAATAGATTTATTAGTGGATCATGTTCTTTATTTTTTGAAGAAAGAAAGCACGTCAAAGTAAGTAAGTTTTTTTCTCTTTAGACACCGATGGTAGGTTTTACTTTTTTCTCCAGTCAAATTCACCACCTTCGATATGAATGTTTGTCTTGCTCCACAATCTTCGTGAGTCTTTGCTAAAACAACTCAAATAGAACCTAGAGATGCAAAAATGACCATTGGTTGAGCTATCTTGTTTTTAGATAGGATGTTCTTACATTTTATTTCCCAAAGTCAACCTTGGTAATGCACAAAAGAAATTTAAAAGTCAAAACAAACACATACTAGCCAA

Coding sequence (CDS)

ATGATTGAGTGGAATCAGTTAAGGACCTTAGGAAAGGCGAGGGATTGGTCCACTTGGTTTGCTTTAGAAATGAAAGCGATAAGTGATGATTGTGAACGGTTCATCTCTTTTCCTTCTGGACATATATATTCAAGCAAACAAGGTGGATTAGATTCATCACTTGTTGCTGCTGTAGCTTCACGTTACTTGGAAGAAATGGAAGGAGCTTACCAATGGGGTTCACAGTTGCACTCCTTTTGCATTGGACTGGAGCTAGTTGGAATCTCAATTGCTGATGTACTTGGTGATGCTTCCTGA

Protein sequence

MIEWNQLRTLGKARDWSTWFALEMKAISDDCERFISFPSGHIYSSKQGGLDSSLVAAVASRYLEEMEGAYQWGSQLHSFCIGLELVGISIADVLGDAS
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo15297.1Spo15297.1mRNA


Homology
[Close]
BLAST of Spo15297.1 vs. NCBI nr
Match: gi|1012364609|gb|KYP75791.1| (Asparagine synthetase [glutamine-hydrolyzing])

HSP 1 Score: 112.5 bits (280), Expect = 4.300e-22
Identity = 65/123 (52.85%), Postives = 70/123 (56.91%), Query Frame = 1 

		  

Query: 10  LGKARDWSTWFALEMKAISDDCERFISFPSGHIYSSKQ---------------------- 69
           LG   D STWFA EMKA+SDDCERFISFP GHIYSSKQ                      
Sbjct: 147 LGWGHDGSTWFASEMKALSDDCERFISFPPGHIYSSKQGGLRRWYNPPWFSEDAVVKRMM 206

Query: 70  ----------GGLDSSLVAAVASRYLEEMEGAYQWGSQLHSFCIGLE-----LVGISIAD 96
                     GGLDSSLVAAVA+RYL E E A QWGSQLH+FCIGL+          +AD
Sbjct: 207 TDVPFGVLLSGGLDSSLVAAVANRYLAESEAARQWGSQLHTFCIGLKGSPDLKAAREVAD 266
[Close]
BLAST of Spo15297.1 vs. NCBI nr
Match: gi|641837626|gb|KDO56577.1| (hypothetical protein CISIN_1g012773mg [Citrus sinensis])

HSP 1 Score: 104.8 bits (260), Expect = 9.000e-20
Identity = 65/140 (46.43%), Postives = 69/140 (49.29%), Query Frame = 1 

		  

Query: 10  LGKARDWSTWFALEMKAISDDCERFISFPSGHIYSSKQ---------------------- 69
           +G   D S WFA EMKA+SDDCERFISFP GHIYSSKQ                      
Sbjct: 32  MGWGLDGSIWFASEMKALSDDCERFISFPPGHIYSSKQGGLRRWYNPPCYSEQIPSNPYD 91

Query: 70  ---------------------------GGLDSSLVAAVASRYLEEMEGAYQWGSQLHSFC 96
                                      GGLDSSLVAAVASRYL + E A QWGSQLHSFC
Sbjct: 92  PLVLRKAFEKAVVKRLMTDVPFGVLLSGGLDSSLVAAVASRYLADSEAACQWGSQLHSFC 151
[Close]
BLAST of Spo15297.1 vs. NCBI nr
Match: gi|567871137|ref|XP_006428190.1| (hypothetical protein CICLE_v10025269mg [Citrus clementina])

HSP 1 Score: 104.8 bits (260), Expect = 9.000e-20
Identity = 65/140 (46.43%), Postives = 69/140 (49.29%), Query Frame = 1 

		  

Query: 10  LGKARDWSTWFALEMKAISDDCERFISFPSGHIYSSKQ---------------------- 69
           +G   D S WFA EMKA+SDDCERFISFP GHIYSSKQ                      
Sbjct: 147 MGWGLDGSIWFASEMKALSDDCERFISFPPGHIYSSKQGGVRRWYNPPWYSEQIPSNPYD 206

Query: 70  ---------------------------GGLDSSLVAAVASRYLEEMEGAYQWGSQLHSFC 96
                                      GGLDSSLVAAVASRYL + E A QWGSQLHSFC
Sbjct: 207 PLVLRKAFEKAVVKRLMTDVPFGVLLSGGLDSSLVAAVASRYLADSEAACQWGSQLHSFC 266
[Close]
BLAST of Spo15297.1 vs. NCBI nr
Match: gi|641837624|gb|KDO56575.1| (hypothetical protein CISIN_1g012773mg [Citrus sinensis])

HSP 1 Score: 104.8 bits (260), Expect = 9.000e-20
Identity = 65/140 (46.43%), Postives = 69/140 (49.29%), Query Frame = 1 

		  

Query: 10  LGKARDWSTWFALEMKAISDDCERFISFPSGHIYSSKQ---------------------- 69
           +G   D S WFA EMKA+SDDCERFISFP GHIYSSKQ                      
Sbjct: 32  MGWGLDGSIWFASEMKALSDDCERFISFPPGHIYSSKQGGLRRWYNPPCYSEQIPSNPYD 91

Query: 70  ---------------------------GGLDSSLVAAVASRYLEEMEGAYQWGSQLHSFC 96
                                      GGLDSSLVAAVASRYL + E A QWGSQLHSFC
Sbjct: 92  PLVLRKAFEKAVVKRLMTDVPFGVLLSGGLDSSLVAAVASRYLADSEAACQWGSQLHSFC 151
[Close]
BLAST of Spo15297.1 vs. NCBI nr
Match: gi|641837627|gb|KDO56578.1| (hypothetical protein CISIN_1g012773mg [Citrus sinensis])

HSP 1 Score: 104.8 bits (260), Expect = 9.000e-20
Identity = 65/140 (46.43%), Postives = 69/140 (49.29%), Query Frame = 1 

		  

Query: 10  LGKARDWSTWFALEMKAISDDCERFISFPSGHIYSSKQ---------------------- 69
           +G   D S WFA EMKA+SDDCERFISFP GHIYSSKQ                      
Sbjct: 32  MGWGLDGSIWFASEMKALSDDCERFISFPPGHIYSSKQGGLRRWYNPPCYSEQIPSNPYD 91

Query: 70  ---------------------------GGLDSSLVAAVASRYLEEMEGAYQWGSQLHSFC 96
                                      GGLDSSLVAAVASRYL + E A QWGSQLHSFC
Sbjct: 92  PLVLRKAFEKAVVKRLMTDVPFGVLLSGGLDSSLVAAVASRYLADSEAACQWGSQLHSFC 151
[Close]
BLAST of Spo15297.1 vs. UniProtKB/TrEMBL
Match: A0A151U9B1_CAJCA (Asparagine synthetase [glutamine-hydrolyzing] OS=Cajanus cajan GN=KK1_019995 PE=4 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 3.000e-22
Identity = 65/123 (52.85%), Postives = 70/123 (56.91%), Query Frame = 1 

		  

Query: 10  LGKARDWSTWFALEMKAISDDCERFISFPSGHIYSSKQ---------------------- 69
           LG   D STWFA EMKA+SDDCERFISFP GHIYSSKQ                      
Sbjct: 147 LGWGHDGSTWFASEMKALSDDCERFISFPPGHIYSSKQGGLRRWYNPPWFSEDAVVKRMM 206

Query: 70  ----------GGLDSSLVAAVASRYLEEMEGAYQWGSQLHSFCIGLE-----LVGISIAD 96
                     GGLDSSLVAAVA+RYL E E A QWGSQLH+FCIGL+          +AD
Sbjct: 207 TDVPFGVLLSGGLDSSLVAAVANRYLAESEAARQWGSQLHTFCIGLKGSPDLKAAREVAD 266
[Close]
BLAST of Spo15297.1 vs. UniProtKB/TrEMBL
Match: A0A0E0E086_9ORYZ (Uncharacterized protein OS=Oryza meridionalis PE=4 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 4.800e-20
Identity = 55/100 (55.00%), Postives = 67/100 (67.00%), Query Frame = 1 

		  

Query: 10  LGKARDWSTWFALEMKAISDDCERFISFPSGHIYSSK---------QGGLDSSLVAAVAS 69
           +G   D S WF+ EMKA+SDDCERFISFP GH+YSSK          GGLDSSLVA+V S
Sbjct: 147 MGWGLDGSVWFSSEMKALSDDCERFISFPPGHLYSSKTDVPFGVLLSGGLDSSLVASVVS 206

Query: 70  RYLEEMEGAYQWGSQLHSFCIGLE-----LVGISIADVLG 96
           R+L E + A QWG++LH+FCIGL+          +AD LG
Sbjct: 207 RHLAEAKVAAQWGNKLHTFCIGLKGSPDLRAAKEVADYLG 246
[Close]
BLAST of Spo15297.1 vs. UniProtKB/TrEMBL
Match: A0A067ERR6_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g012773mg PE=4 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 6.300e-20
Identity = 65/140 (46.43%), Postives = 69/140 (49.29%), Query Frame = 1 

		  

Query: 10  LGKARDWSTWFALEMKAISDDCERFISFPSGHIYSSKQ---------------------- 69
           +G   D S WFA EMKA+SDDCERFISFP GHIYSSKQ                      
Sbjct: 32  MGWGLDGSIWFASEMKALSDDCERFISFPPGHIYSSKQGGLRRWYNPPCYSEQIPSNPYD 91

Query: 70  ---------------------------GGLDSSLVAAVASRYLEEMEGAYQWGSQLHSFC 96
                                      GGLDSSLVAAVASRYL + E A QWGSQLHSFC
Sbjct: 92  PLVLRKAFEKAVVKRLMTDVPFGVLLSGGLDSSLVAAVASRYLADSEAACQWGSQLHSFC 151
[Close]
BLAST of Spo15297.1 vs. UniProtKB/TrEMBL
Match: A0A067ENA6_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g012773mg PE=4 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 6.300e-20
Identity = 65/140 (46.43%), Postives = 69/140 (49.29%), Query Frame = 1 

		  

Query: 10  LGKARDWSTWFALEMKAISDDCERFISFPSGHIYSSKQ---------------------- 69
           +G   D S WFA EMKA+SDDCERFISFP GHIYSSKQ                      
Sbjct: 32  MGWGLDGSIWFASEMKALSDDCERFISFPPGHIYSSKQGGLRRWYNPPCYSEQIPSNPYD 91

Query: 70  ---------------------------GGLDSSLVAAVASRYLEEMEGAYQWGSQLHSFC 96
                                      GGLDSSLVAAVASRYL + E A QWGSQLHSFC
Sbjct: 92  PLVLRKAFEKAVVKRLMTDVPFGVLLSGGLDSSLVAAVASRYLADSEAACQWGSQLHSFC 151
[Close]
BLAST of Spo15297.1 vs. UniProtKB/TrEMBL
Match: A0A067EZB6_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g012773mg PE=4 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 6.300e-20
Identity = 65/140 (46.43%), Postives = 69/140 (49.29%), Query Frame = 1 

		  

Query: 10  LGKARDWSTWFALEMKAISDDCERFISFPSGHIYSSKQ---------------------- 69
           +G   D S WFA EMKA+SDDCERFISFP GHIYSSKQ                      
Sbjct: 32  MGWGLDGSIWFASEMKALSDDCERFISFPPGHIYSSKQGGLRRWYNPPCYSEQIPSNPYD 91

Query: 70  ---------------------------GGLDSSLVAAVASRYLEEMEGAYQWGSQLHSFC 96
                                      GGLDSSLVAAVASRYL + E A QWGSQLHSFC
Sbjct: 92  PLVLRKAFEKAVVKRLMTDVPFGVLLSGGLDSSLVAAVASRYLADSEAACQWGSQLHSFC 151
[Close]
BLAST of Spo15297.1 vs. ExPASy Swiss-Prot
Match: ASNS2_ARATH (Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Arabidopsis thaliana GN=ASN2 PE=2 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 1.400e-20
Identity = 61/140 (43.57%), Postives = 70/140 (50.00%), Query Frame = 1 

		  

Query: 10  LGKARDWSTWFALEMKAISDDCERFISFPSGHIYSSKQ---------------------- 69
           +G   D S WFA EMKA+SDDCE+F+SFP GHIYSSKQ                      
Sbjct: 147 IGWGLDGSVWFASEMKALSDDCEQFMSFPPGHIYSSKQGGLRRWYNPPWYNEQVPSTPYD 206

Query: 70  ---------------------------GGLDSSLVAAVASRYLEEMEGAYQWGSQLHSFC 96
                                      GGLDSSLVAAVA R+LE+ E A QWGSQLH+FC
Sbjct: 207 PLVLRNAFEKAVIKRLMTDVPFGVLLSGGLDSSLVAAVALRHLEKSEAARQWGSQLHTFC 266
[Close]
BLAST of Spo15297.1 vs. ExPASy Swiss-Prot
Match: ASNS3_ARATH (Asparagine synthetase [glutamine-hydrolyzing] 3 OS=Arabidopsis thaliana GN=ASN3 PE=2 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 4.500e-19
Identity = 58/140 (41.43%), Postives = 69/140 (49.29%), Query Frame = 1 

		  

Query: 10  LGKARDWSTWFALEMKAISDDCERFISFPSGHIYSSKQ---------------------- 69
           +G   D S WFA EMKA+SDDCE+F+ FP GHIYSSKQ                      
Sbjct: 147 IGWGLDGSVWFASEMKALSDDCEQFMCFPPGHIYSSKQGGLRRWYNPPWFSEVVPSTPYD 206

Query: 70  ---------------------------GGLDSSLVAAVASRYLEEMEGAYQWGSQLHSFC 96
                                      GGLDSSLVA+VA R+LE+ E A QWGS+LH+FC
Sbjct: 207 PLVVRNTFEKAVIKRLMTDVPFGVLLSGGLDSSLVASVALRHLEKSEAACQWGSKLHTFC 266
[Close]
BLAST of Spo15297.1 vs. ExPASy Swiss-Prot
Match: ASNS2_ORYSJ (Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Oryza sativa subsp. japonica GN=Os06g0265000 PE=2 SV=3)

HSP 1 Score: 89.7 bits (221), Expect = 1.900e-17
Identity = 55/140 (39.29%), Postives = 67/140 (47.86%), Query Frame = 1 

		  

Query: 10  LGKARDWSTWFALEMKAISDDCERFISFPSGHIYSSK----------------------- 69
           +G   D S WF+ EMKA+SDDCERFISFP GH+YSSK                       
Sbjct: 147 MGWGLDGSVWFSSEMKALSDDCERFISFPPGHLYSSKTGGLRRWYNPPWFSESIPSTPYN 206

Query: 70  --------------------------QGGLDSSLVAAVASRYLEEMEGAYQWGSQLHSFC 96
                                      GGLDSSLVA+V SR+L E + A QWG++LH+FC
Sbjct: 207 PLLLRQSFEKAIIKRLMTDVPFGVLLSGGLDSSLVASVVSRHLAEAKVAAQWGNKLHTFC 266
[Close]
BLAST of Spo15297.1 vs. ExPASy Swiss-Prot
Match: ASNS2_LOTJA (Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Lotus japonicus GN=AS2 PE=2 SV=2)

HSP 1 Score: 83.2 bits (204), Expect = 1.800e-15
Identity = 46/124 (37.10%), Postives = 58/124 (46.77%), Query Frame = 1 

		  

Query: 10  LGKARDWSTWFALEMKAISDDCERFISFPSGHIYSSKQ---------------------- 69
           +G   D S W A E+K ++DDCE F  FP GH+YSSK+                      
Sbjct: 147 IGYGLDGSVWIASELKGLNDDCEHFELFPPGHLYSSKEKEFRRWYNPPWFSEAIPSAPYD 206

Query: 70  ---------------------------GGLDSSLVAAVASRYLEEMEGAYQWGSQLHSFC 85
                                      GGLDSSLVA+V +RYL + + A QWGS+LHSFC
Sbjct: 207 PLALRQAFEKAIIKRLMTDVPFGVLLSGGLDSSLVASVTARYLADTKAAKQWGSKLHSFC 266
[Close]
BLAST of Spo15297.1 vs. ExPASy Swiss-Prot
Match: ASNS_SANAU (Asparagine synthetase [glutamine-hydrolyzing] OS=Sandersonia aurantiaca GN=AND1 PE=2 SV=3)

HSP 1 Score: 81.3 bits (199), Expect = 6.700e-15
Identity = 46/124 (37.10%), Postives = 57/124 (45.97%), Query Frame = 1 

		  

Query: 10  LGKARDWSTWFALEMKAISDDCERFISFPSGHIYSSKQ---------------------- 69
           +G   D S W + EMK ++DDCE F  FP GH+YSSK+                      
Sbjct: 147 IGWGLDGSVWISSEMKGLNDDCEHFKFFPPGHLYSSKEGSFKRWYNPPWFSEVIPSVPFD 206

Query: 70  ---------------------------GGLDSSLVAAVASRYLEEMEGAYQWGSQLHSFC 85
                                      GGLDSSLVA+V +RYLE  + A  WG+QLHSFC
Sbjct: 207 PLALRKAFEDAVIKRLMTDVPFGVLLSGGLDSSLVASVTARYLEGTKAAELWGTQLHSFC 266
[Close]
BLAST of Spo15297.1 vs. TAIR (Arabidopsis)
Match: AT5G65010.2 (asparagine synthetase 2)

HSP 1 Score: 100.1 bits (248), Expect = 7.800e-22
Identity = 61/140 (43.57%), Postives = 70/140 (50.00%), Query Frame = 1 

		  

Query: 10  LGKARDWSTWFALEMKAISDDCERFISFPSGHIYSSKQ---------------------- 69
           +G   D S WFA EMKA+SDDCE+F+SFP GHIYSSKQ                      
Sbjct: 147 IGWGLDGSVWFASEMKALSDDCEQFMSFPPGHIYSSKQGGLRRWYNPPWYNEQVPSTPYD 206

Query: 70  ---------------------------GGLDSSLVAAVASRYLEEMEGAYQWGSQLHSFC 96
                                      GGLDSSLVAAVA R+LE+ E A QWGSQLH+FC
Sbjct: 207 PLVLRNAFEKAVIKRLMTDVPFGVLLSGGLDSSLVAAVALRHLEKSEAARQWGSQLHTFC 266
[Close]
BLAST of Spo15297.1 vs. TAIR (Arabidopsis)
Match: AT5G10240.1 (asparagine synthetase 3)

HSP 1 Score: 95.1 bits (235), Expect = 2.500e-20
Identity = 58/140 (41.43%), Postives = 69/140 (49.29%), Query Frame = 1 

		  

Query: 10  LGKARDWSTWFALEMKAISDDCERFISFPSGHIYSSKQ---------------------- 69
           +G   D S WFA EMKA+SDDCE+F+ FP GHIYSSKQ                      
Sbjct: 147 IGWGLDGSVWFASEMKALSDDCEQFMCFPPGHIYSSKQGGLRRWYNPPWFSEVVPSTPYD 206

Query: 70  ---------------------------GGLDSSLVAAVASRYLEEMEGAYQWGSQLHSFC 96
                                      GGLDSSLVA+VA R+LE+ E A QWGS+LH+FC
Sbjct: 207 PLVVRNTFEKAVIKRLMTDVPFGVLLSGGLDSSLVASVALRHLEKSEAACQWGSKLHTFC 266
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BLAST of Spo15297.1 vs. TAIR (Arabidopsis)
Match: AT3G47340.1 (glutamine-dependent asparagine synthase 1)

HSP 1 Score: 69.7 bits (169), Expect = 1.100e-12
Identity = 44/140 (31.43%), Postives = 57/140 (40.71%), Query Frame = 1 

		  

Query: 10  LGKARDWSTWFALEMKAISDDCERFISFPSGHIYSSKQGGLDSS---------------- 69
           +G   D S W + EMK ++DDCE F +FP GH YSSK GG                    
Sbjct: 147 IGWGLDGSVWISSEMKGLNDDCEHFETFPPGHFYSSKLGGFKQWYNPPWFNESVPSTPYE 206

Query: 70  ---------------------------------LVAAVASRYLEEMEGAYQWGSQLHSFC 96
                                            LVA++ +R+L   + A QWG QLHSFC
Sbjct: 207 PLAIRRAFENAVIKRLMTDVPFGVLLSGGLDSSLVASITARHLAGTKAAKQWGPQLHSFC 266
The following BLAST results are available for this feature:
BLAST of Spo15297.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|1012364609|gb|KYP75791.1|4.3e-2252.8Asparagine synthetase [glutami... [more]
gi|641837626|gb|KDO56577.1|9.0e-2046.4hypothetical protein CISIN_1g0... [more]
gi|567871137|ref|XP_006428190.1|9.0e-2046.4hypothetical protein CICLE_v10... [more]
gi|641837624|gb|KDO56575.1|9.0e-2046.4hypothetical protein CISIN_1g0... [more]
gi|641837627|gb|KDO56578.1|9.0e-2046.4hypothetical protein CISIN_1g0... [more]
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BLAST of Spo15297.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A151U9B1_CAJCA3.0e-2252.8Asparagine synthetase [glutami... [more]
A0A0E0E086_9ORYZ4.8e-2055.0Uncharacterized protein OS=Ory... [more]
A0A067ERR6_CITSI6.3e-2046.4Uncharacterized protein OS=Cit... [more]
A0A067ENA6_CITSI6.3e-2046.4Uncharacterized protein OS=Cit... [more]
A0A067EZB6_CITSI6.3e-2046.4Uncharacterized protein OS=Cit... [more]
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BLAST of Spo15297.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5
Match NameE-valueIdentityDescription
ASNS2_ARATH1.4e-2043.5Asparagine synthetase [glutami... [more]
ASNS3_ARATH4.5e-1941.4Asparagine synthetase [glutami... [more]
ASNS2_ORYSJ1.9e-1739.2Asparagine synthetase [glutami... [more]
ASNS2_LOTJA1.8e-1537.1Asparagine synthetase [glutami... [more]
ASNS_SANAU6.7e-1537.1Asparagine synthetase [glutami... [more]
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BLAST of Spo15297.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 3
Match NameE-valueIdentityDescription
AT5G65010.27.8e-2243.5asparagine synthetase 2[more]
AT5G10240.12.5e-2041.4asparagine synthetase 3[more]
AT3G47340.11.1e-1231.4glutamine-dependent asparagine... [more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001962Asparagine synthasePFAMPF00733Asn_synthasecoord: 48..83
score: 7.
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620coord: 48..85
score: 1.
NoneNo IPR availablePANTHERPTHR11772ASPARAGINE SYNTHETASEcoord: 10..84
score: 7.1
NoneNo IPR availablePANTHERPTHR11772:SF13ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] 2-RELATEDcoord: 10..84
score: 7.1

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006529 asparagine biosynthetic process
biological_process GO:0008152 metabolic process
biological_process GO:0006189 'de novo' IMP biosynthetic process
biological_process GO:0009113 purine nucleobase biosynthetic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0009535 chloroplast thylakoid membrane
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0016879 ligase activity, forming carbon-nitrogen bonds
molecular_function GO:0004066 asparagine synthase (glutamine-hydrolyzing) activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0005516 calmodulin binding
molecular_function GO:0008568 microtubule-severing ATPase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004637 phosphoribosylamine-glycine ligase activity
RNA-Seq Expression