Spo18232 (gene) | SpinachBase

Overview


Name	Spo18232
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit
Location	chr4 : 122015866 .. 122016294 (-)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACACCGACATCACTGCATCAACAAAGCCCGAATTCAAAGTAGTCGATCGGAACCCACCATTCACCAAAGTCGTCGGAAACTTCAGCTCCCTCGATTACCTCCGTTTCGTCACTATTACCGGCGTTTCTGTCACCGTCGGATACTTATCCGGTAATCCCTTTTCCTTTATCCTCATTTTATCAAATACCCATTTCGCAAATTCCTCTCTTTAATTTTTTTTTCTGCTTTTTTTTTGTGATTTAGGGATTAAGCCTGGGATTAAAGGTCCTTCAATGGTGACGGGCGGGCTAATCGGGGTGATGGGAGGGTTCATGTATGCTTACCAGAATTCTGCTGGCAGACTCATGGGTTTCTTTCCTAACGATGACGAGGTAGTTAAGTACAAGAAGAAGCTTAATCAGTAATTTTATCTTATGAAATGGGAA

mRNA sequence

ATGAACACCGACATCACTGCATCAACAAAGCCCGAATTCAAAGTAGTCGATCGGAACCCACCATTCACCAAAGTCGTCGGAAACTTCAGCTCCCTCGATTACCTCCGTTTCGTCACTATTACCGGCGTTTCTGTCACCGTCGGATACTTATCCGGGATTAAGCCTGGGATTAAAGGTCCTTCAATGGTGACGGGCGGGCTAATCGGGGTGATGGGAGGGTTCATGTATGCTTACCAGAATTCTGCTGGCAGACTCATGGGTTTCTTTCCTAACGATGACGAGGTAGTTAAGTACAAGAAGAAGCTTAATCAGTAATTTTATCTTATGAAATGGGAA

Coding sequence (CDS)

ATGAACACCGACATCACTGCATCAACAAAGCCCGAATTCAAAGTAGTCGATCGGAACCCACCATTCACCAAAGTCGTCGGAAACTTCAGCTCCCTCGATTACCTCCGTTTCGTCACTATTACCGGCGTTTCTGTCACCGTCGGATACTTATCCGGGATTAAGCCTGGGATTAAAGGTCCTTCAATGGTGACGGGCGGGCTAATCGGGGTGATGGGAGGGTTCATGTATGCTTACCAGAATTCTGCTGGCAGACTCATGGGTTTCTTTCCTAACGATGACGAGGTAGTTAAGTACAAGAAGAAGCTTAATCAGTAA

Protein sequence

MNTDITASTKPEFKVVDRNPPFTKVVGNFSSLDYLRFVTITGVSVTVGYLSGIKPGIKGPSMVTGGLIGVMGGFMYAYQNSAGRLMGFFPNDDEVVKYKKKLNQ

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo18232.1	Spo18232.1	mRNA

Homology

[Close]

BLAST of Spo18232.1 vs. NCBI nr
Match: gi|902225342|gb|KNA20036.1| (hypothetical protein SOVF_056130 isoform A [Spinacia oleracea])

HSP 1 Score: 211.1 bits (536), Expect = 9.400e-52
Identity = 104/104 (100.00%), Postives = 104/104 (100.00%), Query Frame = 1


		  
Query: 1   MNTDITASTKPEFKVVDRNPPFTKVVGNFSSLDYLRFVTITGVSVTVGYLSGIKPGIKGP 60
           MNTDITASTKPEFKVVDRNPPFTKVVGNFSSLDYLRFVTITGVSVTVGYLSGIKPGIKGP
Sbjct: 1   MNTDITASTKPEFKVVDRNPPFTKVVGNFSSLDYLRFVTITGVSVTVGYLSGIKPGIKGP 60

Query: 61  SMVTGGLIGVMGGFMYAYQNSAGRLMGFFPNDDEVVKYKKKLNQ 105
           SMVTGGLIGVMGGFMYAYQNSAGRLMGFFPNDDEVVKYKKKLNQ
Sbjct: 61  SMVTGGLIGVMGGFMYAYQNSAGRLMGFFPNDDEVVKYKKKLNQ 104

[Close]

BLAST of Spo18232.1 vs. NCBI nr
Match: gi|902212875|gb|KNA17110.1| (hypothetical protein SOVF_083060 [Spinacia oleracea])

HSP 1 Score: 202.2 bits (513), Expect = 4.400e-49
Identity = 99/104 (95.19%), Postives = 102/104 (98.08%), Query Frame = 1


		  
Query: 1   MNTDITASTKPEFKVVDRNPPFTKVVGNFSSLDYLRFVTITGVSVTVGYLSGIKPGIKGP 60
           MNTDITASTKPE+ V+DRNP FTKVVGNFSSLDYLRFVTITGVSVTVGYLSGIKPGIKGP
Sbjct: 1   MNTDITASTKPEYTVIDRNPLFTKVVGNFSSLDYLRFVTITGVSVTVGYLSGIKPGIKGP 60

Query: 61  SMVTGGLIGVMGGFMYAYQNSAGRLMGFFPNDDEVVKYKKKLNQ 105
           SMVTGGLIGV+GGFMYAYQNSAGRLMGFFPNDDEVVKYKKKLNQ
Sbjct: 61  SMVTGGLIGVLGGFMYAYQNSAGRLMGFFPNDDEVVKYKKKLNQ 104

[Close]

BLAST of Spo18232.1 vs. NCBI nr
Match: gi|731329987|ref|XP_010675885.1| (PREDICTED: NADH-ubiquinone oxidoreductase 20.9 kDa subunit [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 200.7 bits (509), Expect = 1.300e-48
Identity = 97/104 (93.27%), Postives = 102/104 (98.08%), Query Frame = 1


		  
Query: 1   MNTDITASTKPEFKVVDRNPPFTKVVGNFSSLDYLRFVTITGVSVTVGYLSGIKPGIKGP 60
           MNTDITASTKPE+ VVDRNPPFTKVVGNFSSLDYLRFVTITGVSVTVGYLSGIKPGIKGP
Sbjct: 1   MNTDITASTKPEYTVVDRNPPFTKVVGNFSSLDYLRFVTITGVSVTVGYLSGIKPGIKGP 60

Query: 61  SMVTGGLIGVMGGFMYAYQNSAGRLMGFFPNDDEVVKYKKKLNQ 105
           SMVTGGLIG+MGGFMYAYQNSAGRLMGFFPN+DEV +YKK+LNQ
Sbjct: 61  SMVTGGLIGLMGGFMYAYQNSAGRLMGFFPNEDEVARYKKQLNQ 104

[Close]

BLAST of Spo18232.1 vs. NCBI nr
Match: gi|902225343|gb|KNA20037.1| (hypothetical protein SOVF_056130 isoform B [Spinacia oleracea])

HSP 1 Score: 193.7 bits (491), Expect = 1.600e-46
Identity = 95/95 (100.00%), Postives = 95/95 (100.00%), Query Frame = 1


		  
Query: 1  MNTDITASTKPEFKVVDRNPPFTKVVGNFSSLDYLRFVTITGVSVTVGYLSGIKPGIKGP 60
          MNTDITASTKPEFKVVDRNPPFTKVVGNFSSLDYLRFVTITGVSVTVGYLSGIKPGIKGP
Sbjct: 1  MNTDITASTKPEFKVVDRNPPFTKVVGNFSSLDYLRFVTITGVSVTVGYLSGIKPGIKGP 60

Query: 61 SMVTGGLIGVMGGFMYAYQNSAGRLMGFFPNDDEV 96
          SMVTGGLIGVMGGFMYAYQNSAGRLMGFFPNDDEV
Sbjct: 61 SMVTGGLIGVMGGFMYAYQNSAGRLMGFFPNDDEV 95

[Close]

BLAST of Spo18232.1 vs. NCBI nr
Match: gi|351720975|ref|NP_001237706.1| (uncharacterized protein LOC100306111 [Glycine max])

HSP 1 Score: 193.4 bits (490), Expect = 2.000e-46
Identity = 92/101 (91.09%), Postives = 98/101 (97.03%), Query Frame = 1


		  
Query: 1   MNTDITASTKPEFKVVDRNPPFTKVVGNFSSLDYLRFVTITGVSVTVGYLSGIKPGIKGP 60
           MNTDITASTKPE+ V+DRNPPFTKVVGNF++LDYLRFVTITGVSVTVGYLSGIKPGI+GP
Sbjct: 1   MNTDITASTKPEYPVIDRNPPFTKVVGNFNTLDYLRFVTITGVSVTVGYLSGIKPGIRGP 60

Query: 61  SMVTGGLIGVMGGFMYAYQNSAGRLMGFFPNDDEVVKYKKK 102
           SMVTGGLIGVMGGFMYAYQNSAGRLMGFFPNDDEV +Y KK
Sbjct: 61  SMVTGGLIGVMGGFMYAYQNSAGRLMGFFPNDDEVARYNKK 101

[Close]

BLAST of Spo18232.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RKJ6_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_056130 PE=4 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 6.600e-52
Identity = 104/104 (100.00%), Postives = 104/104 (100.00%), Query Frame = 1


		  
Query: 1   MNTDITASTKPEFKVVDRNPPFTKVVGNFSSLDYLRFVTITGVSVTVGYLSGIKPGIKGP 60
           MNTDITASTKPEFKVVDRNPPFTKVVGNFSSLDYLRFVTITGVSVTVGYLSGIKPGIKGP
Sbjct: 1   MNTDITASTKPEFKVVDRNPPFTKVVGNFSSLDYLRFVTITGVSVTVGYLSGIKPGIKGP 60

Query: 61  SMVTGGLIGVMGGFMYAYQNSAGRLMGFFPNDDEVVKYKKKLNQ 105
           SMVTGGLIGVMGGFMYAYQNSAGRLMGFFPNDDEVVKYKKKLNQ
Sbjct: 61  SMVTGGLIGVMGGFMYAYQNSAGRLMGFFPNDDEVVKYKKKLNQ 104

[Close]

BLAST of Spo18232.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RCB4_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_083060 PE=4 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 3.100e-49
Identity = 99/104 (95.19%), Postives = 102/104 (98.08%), Query Frame = 1


		  
Query: 1   MNTDITASTKPEFKVVDRNPPFTKVVGNFSSLDYLRFVTITGVSVTVGYLSGIKPGIKGP 60
           MNTDITASTKPE+ V+DRNP FTKVVGNFSSLDYLRFVTITGVSVTVGYLSGIKPGIKGP
Sbjct: 1   MNTDITASTKPEYTVIDRNPLFTKVVGNFSSLDYLRFVTITGVSVTVGYLSGIKPGIKGP 60

Query: 61  SMVTGGLIGVMGGFMYAYQNSAGRLMGFFPNDDEVVKYKKKLNQ 105
           SMVTGGLIGV+GGFMYAYQNSAGRLMGFFPNDDEVVKYKKKLNQ
Sbjct: 61  SMVTGGLIGVLGGFMYAYQNSAGRLMGFFPNDDEVVKYKKKLNQ 104

[Close]

BLAST of Spo18232.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CLI3_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_4g089980 PE=4 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 8.900e-49
Identity = 97/104 (93.27%), Postives = 102/104 (98.08%), Query Frame = 1


		  
Query: 1   MNTDITASTKPEFKVVDRNPPFTKVVGNFSSLDYLRFVTITGVSVTVGYLSGIKPGIKGP 60
           MNTDITASTKPE+ VVDRNPPFTKVVGNFSSLDYLRFVTITGVSVTVGYLSGIKPGIKGP
Sbjct: 1   MNTDITASTKPEYTVVDRNPPFTKVVGNFSSLDYLRFVTITGVSVTVGYLSGIKPGIKGP 60

Query: 61  SMVTGGLIGVMGGFMYAYQNSAGRLMGFFPNDDEVVKYKKKLNQ 105
           SMVTGGLIG+MGGFMYAYQNSAGRLMGFFPN+DEV +YKK+LNQ
Sbjct: 61  SMVTGGLIGLMGGFMYAYQNSAGRLMGFFPNEDEVARYKKQLNQ 104

[Close]

BLAST of Spo18232.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RMF3_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_056130 PE=4 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 1.100e-46
Identity = 95/95 (100.00%), Postives = 95/95 (100.00%), Query Frame = 1


		  
Query: 1  MNTDITASTKPEFKVVDRNPPFTKVVGNFSSLDYLRFVTITGVSVTVGYLSGIKPGIKGP 60
          MNTDITASTKPEFKVVDRNPPFTKVVGNFSSLDYLRFVTITGVSVTVGYLSGIKPGIKGP
Sbjct: 1  MNTDITASTKPEFKVVDRNPPFTKVVGNFSSLDYLRFVTITGVSVTVGYLSGIKPGIKGP 60

Query: 61 SMVTGGLIGVMGGFMYAYQNSAGRLMGFFPNDDEV 96
          SMVTGGLIGVMGGFMYAYQNSAGRLMGFFPNDDEV
Sbjct: 61 SMVTGGLIGVMGGFMYAYQNSAGRLMGFFPNDDEV 95

[Close]

BLAST of Spo18232.1 vs. UniProtKB/TrEMBL
Match: A0A151T6J4_CAJCA (Uncharacterized protein OS=Cajanus cajan GN=KK1_017204 PE=4 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 1.400e-46
Identity = 92/101 (91.09%), Postives = 98/101 (97.03%), Query Frame = 1


		  
Query: 1   MNTDITASTKPEFKVVDRNPPFTKVVGNFSSLDYLRFVTITGVSVTVGYLSGIKPGIKGP 60
           MNTDITASTKPE+ V+DRNPPFTKVVGNF++LDYLRFVTITGVSVTVGYLSGIKPGI+GP
Sbjct: 1   MNTDITASTKPEYPVIDRNPPFTKVVGNFNTLDYLRFVTITGVSVTVGYLSGIKPGIRGP 60

Query: 61  SMVTGGLIGVMGGFMYAYQNSAGRLMGFFPNDDEVVKYKKK 102
           SMVTGGLIGVMGGFMYAYQNSAGRLMGFFPNDDEV +Y KK
Sbjct: 61  SMVTGGLIGVMGGFMYAYQNSAGRLMGFFPNDDEVARYNKK 101

[Close]

BLAST of Spo18232.1 vs. TAIR (Arabidopsis)
Match: AT4G16450.1 (unknown protein)

HSP 1 Score: 176.0 bits (445), Expect = 1.200e-44
Identity = 85/101 (84.16%), Postives = 92/101 (91.09%), Query Frame = 1


		  
Query: 1   MNTDITASTKPEFKVVDRNPPFTKVVGNFSSLDYLRFVTITGVSVTVGYLSGIKPGIKGP 60
           MNTDITA  K ++ VVDRNP FTKVVGNFS+LDYLRF TITG+SVTVGYLSGIKPGIKGP
Sbjct: 1   MNTDITALEKAQYPVVDRNPAFTKVVGNFSTLDYLRFSTITGISVTVGYLSGIKPGIKGP 60

Query: 61  SMVTGGLIGVMGGFMYAYQNSAGRLMGFFPNDDEVVKYKKK 102
           SMVTGGLIG+MGGFMYAYQNSAGRLMGFFPND EV  Y+K+
Sbjct: 61  SMVTGGLIGLMGGFMYAYQNSAGRLMGFFPNDGEVASYQKR 101

The following BLAST results are available for this feature:

BLAST of Spo18232.1 vs. NCBI nr
BLAST of Spo18232.1 vs. UniProtKB/TrEMBL
BLAST of Spo18232.1 vs. ExPASy Swiss-Prot
BLAST of Spo18232.1 vs. TAIR (Arabidopsis)

BLAST of Spo18232.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902225342\|gb\|KNA20036.1\|	9.4e-52	100.	hypothetical protein SOVF_0561...	[more]
gi\|902212875\|gb\|KNA17110.1\|	4.4e-49	95.1	hypothetical protein SOVF_0830...	[more]
gi\|731329987\|ref\|XP_010675885.1\|	1.3e-48	93.2	PREDICTED: NADH-ubiquinone oxi...	[more]
gi\|902225343\|gb\|KNA20037.1\|	1.6e-46	100.	hypothetical protein SOVF_0561...	[more]
gi\|351720975\|ref\|NP_001237706.1\|	2.0e-46	91.0	uncharacterized protein LOC100...	[more]

BLAST of Spo18232.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RKJ6_SPIOL	6.6e-52	100.	Uncharacterized protein OS=Spi...	[more]
A0A0K9RCB4_SPIOL	3.1e-49	95.1	Uncharacterized protein OS=Spi...	[more]
A0A0J8CLI3_BETVU	8.9e-49	93.2	Uncharacterized protein OS=Bet...	[more]
A0A0K9RMF3_SPIOL	1.1e-46	100.	Uncharacterized protein OS=Spi...	[more]
A0A151T6J4_CAJCA	1.4e-46	91.0	Uncharacterized protein OS=Caj...	[more]

BLAST of Spo18232.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Spo18232.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1


Match Name	E-value	Identity	Description
AT4G16450.1	1.2e-44	84.1	unknown protein	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019721	NADH-ubiquinone oxidoreductase, 21kDa subunit, N-terminal	PFAM	PF10785	NADH-u_ox-rdase	coord: 11..89 score: 2.7
None	No IPR available	PANTHER	PTHR34062	FAMILY NOT NAMED	coord: 1..104 score: 1.0
None	No IPR available	PANTHER	PTHR34062:SF1	SUBFAMILY NOT NAMED	coord: 1..104 score: 1.0

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009853	photorespiration
biological_process	GO:0006412	translation
biological_process	GO:0010027	thylakoid membrane organization
biological_process	GO:0009636	response to toxic substance
biological_process	GO:0010583	response to cyclopentenone
biological_process	GO:0010731	protein glutathionylation
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0019852	L-ascorbic acid metabolic process
biological_process	GO:0006749	glutathione metabolic process
biological_process	GO:0080129	proteasome core complex assembly
biological_process	GO:0042254	ribosome biogenesis
biological_process	GO:0015994	chlorophyll metabolic process
biological_process	GO:0016126	sterol biosynthetic process
biological_process	GO:0016226	iron-sulfur cluster assembly
biological_process	GO:0008152	metabolic process
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
biological_process	GO:0051788	response to misfolded protein
biological_process	GO:0009407	toxin catabolic process
cellular_component	GO:0009941	chloroplast envelope
cellular_component	GO:0005840	ribosome
cellular_component	GO:0010007	magnesium chelatase complex
cellular_component	GO:0009570	chloroplast stroma
cellular_component	GO:0005747	mitochondrial respiratory chain complex I
molecular_function	GO:0004364	glutathione transferase activity
molecular_function	GO:0016851	magnesium chelatase activity
molecular_function	GO:0003735	structural constituent of ribosome
molecular_function	GO:0045174	glutathione dehydrogenase (ascorbate) activity
molecular_function	GO:0005515	protein binding
molecular_function	GO:0005198	structural molecule activity
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016757	transferase activity, transferring glycosyl groups
molecular_function	GO:0046422	violaxanthin de-epoxidase activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo08505	0.98	Barchart \| Table
Spo09321	0.71	Barchart \| Table
Spo09384	0.71	Barchart \| Table
Spo17668	0.69	Barchart \| Table
Spo01967	0.68	Barchart \| Table
Spo18000	0.68	Barchart \| Table
Spo02663	0.67	Barchart \| Table
Spo18336	0.66	Barchart \| Table
Spo16392	0.65	Barchart \| Table
Spo05810	0.65	Barchart \| Table