Spo18256 (gene) | SpinachBase

Overview


Name	Spo18256
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Unknown protein
Location	chr4 : 122529320 .. 122529703 (-)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCAACTGACGTGAACAGCTCCTTCCATCCACACCCAGCACCGCCATCTCCAGACTCAAGTTCTTCAACAGTTATTGTCATCGTTGTCATCTCGTTTGGGTGTCTCTTCTTAACCGGAGCTGTTTTGTCATGCATTTGGTTGTTGATGAAGAGGCGCAAGGAGCGGAAACTTATTCGTGAGAGGAAAGACATCGATGTGGATGAGCATTTGAAAGTGCATGAAATGATCGTACGAGGGCCGCACGGCCCAAAGAAGGTGTTAGTCTCGGTGGATGACGATGTACGTGTGCATGAGGATGTTCACAGGAGTGAGGTTGATATGGTCGGTAAAGGAAAGCACGGAGAGGCCAGTGTAAGTCTTGGTACTAGCCAAGTTTGA

mRNA sequence

ATGGCTTCAACTGACGTGAACAGCTCCTTCCATCCACACCCAGCACCGCCATCTCCAGACTCAAGTTCTTCAACAGTTATTGTCATCGTTGTCATCTCGTTTGGGTGTCTCTTCTTAACCGGAGCTGTTTTGTCATGCATTTGGTTGTTGATGAAGAGGCGCAAGGAGCGGAAACTTATTCGTGAGAGGAAAGACATCGATGTGGATGAGCATTTGAAAGTGCATGAAATGATCGTACGAGGGCCGCACGGCCCAAAGAAGGTGTTAGTCTCGGTGGATGACGATGTACGTGTGCATGAGGATGTTCACAGGAGTGAGGTTGATATGGTCGGTAAAGGAAAGCACGGAGAGGCCAGTGTAAGTCTTGGTACTAGCCAAGTTTGA

Coding sequence (CDS)

ATGGCTTCAACTGACGTGAACAGCTCCTTCCATCCACACCCAGCACCGCCATCTCCAGACTCAAGTTCTTCAACAGTTATTGTCATCGTTGTCATCTCGTTTGGGTGTCTCTTCTTAACCGGAGCTGTTTTGTCATGCATTTGGTTGTTGATGAAGAGGCGCAAGGAGCGGAAACTTATTCGTGAGAGGAAAGACATCGATGTGGATGAGCATTTGAAAGTGCATGAAATGATCGTACGAGGGCCGCACGGCCCAAAGAAGGTGTTAGTCTCGGTGGATGACGATGTACGTGTGCATGAGGATGTTCACAGGAGTGAGGTTGATATGGTCGGTAAAGGAAAGCACGGAGAGGCCAGTGTAAGTCTTGGTACTAGCCAAGTTTGA

Protein sequence

MASTDVNSSFHPHPAPPSPDSSSSTVIVIVVISFGCLFLTGAVLSCIWLLMKRRKERKLIRERKDIDVDEHLKVHEMIVRGPHGPKKVLVSVDDDVRVHEDVHRSEVDMVGKGKHGEASVSLGTSQV

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo18256.1	Spo18256.1	mRNA

Homology

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BLAST of Spo18256.1 vs. NCBI nr
Match: gi|902140135|gb|KNA04616.1| (hypothetical protein SOVF_198040 [Spinacia oleracea])

HSP 1 Score: 235.0 bits (598), Expect = 7.500e-59
Identity = 119/119 (100.00%), Postives = 119/119 (100.00%), Query Frame = 1


		  
Query: 1   MASTDVNSSFHPHPAPPSPDSSSSTVIVIVVISFGCLFLTGAVLSCIWLLMKRRKERKLI 60
           MASTDVNSSFHPHPAPPSPDSSSSTVIVIVVISFGCLFLTGAVLSCIWLLMKRRKERKLI
Sbjct: 1   MASTDVNSSFHPHPAPPSPDSSSSTVIVIVVISFGCLFLTGAVLSCIWLLMKRRKERKLI 60

Query: 61  RERKDIDVDEHLKVHEMIVRGPHGPKKVLVSVDDDVRVHEDVHRSEVDMVGKGKHGEAS 120
           RERKDIDVDEHLKVHEMIVRGPHGPKKVLVSVDDDVRVHEDVHRSEVDMVGKGKHGEAS
Sbjct: 61  RERKDIDVDEHLKVHEMIVRGPHGPKKVLVSVDDDVRVHEDVHRSEVDMVGKGKHGEAS 119

[Close]

BLAST of Spo18256.1 vs. NCBI nr
Match: gi|870868643|gb|KMT19446.1| (hypothetical protein BVRB_1g010760 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 153.3 bits (386), Expect = 2.900e-34
Identity = 83/112 (74.11%), Postives = 91/112 (81.25%), Query Frame = 1


		  
Query: 11  HPHPA----PPSPDSSSSTVIVIVVISFGCLFLTGAVLSCIWLLM-KRRKERKLIRERKD 70
           HPHP     PPS D S  TVIVI+ ISFGCLFL GA+L CIW  M K+R ERKLI ERKD
Sbjct: 19  HPHPTPVPPPPSLD-SHPTVIVIIFISFGCLFLLGALLLCIWWFMKKKRTERKLINERKD 78

Query: 71  IDVDEHLKVHEMIVRGPHGPKKVLVSVDDDVRVHEDVHRSEVDMVGKGKHGE 118
           IDVDEHLKV EMI+ G HGPKKVLVSV+D++RVHEDVHRSEVDMVGKG HG+
Sbjct: 79  IDVDEHLKVQEMIMAGRHGPKKVLVSVEDEIRVHEDVHRSEVDMVGKGLHGK 129

[Close]

BLAST of Spo18256.1 vs. NCBI nr
Match: gi|976900015|gb|KVH88308.1| (hypothetical protein Ccrd_024163 [Cynara cardunculus var. scolymus])

HSP 1 Score: 89.4 bits (220), Expect = 5.100e-15
Identity = 43/109 (39.45%), Postives = 74/109 (67.89%), Query Frame = 1


		  
Query: 12  PHPAPPSPDSSSSTVIVIVVISFGCLFLTGAVLSCIWLLMKRRKERKLIRERKDIDVDEH 71
           P P PPSP  + +T+IV+V +S G +F    +++ +W  +K++K  K++R+ +++ VDEH
Sbjct: 110 PPPPPPSP-GNHTTIIVVVFVSCGGVFFLAFIMAALWCFLKKKK--KMVRKAENVHVDEH 169

Query: 72  LKVHEMIVRGPHGPKKVLVSVDDDVRVHEDVHRSEVDMVGKGKHGEASV 121
             V E IV+GPHG + V++SV+DD+ + ED+ +SE++   KG H  + V
Sbjct: 170 RTVKESIVQGPHGTETVMLSVEDDIHIEEDIRKSEIENFRKGLHLNSGV 215

[Close]

BLAST of Spo18256.1 vs. NCBI nr
Match: gi|802559251|ref|XP_012066081.1| (PREDICTED: proline-rich receptor-like protein kinase PERK2 [Jatropha curcas])

HSP 1 Score: 86.3 bits (212), Expect = 4.300e-14
Identity = 41/95 (43.16%), Postives = 65/95 (68.42%), Query Frame = 1


		  
Query: 12  PHPAPPSPDSSSSTVIVIVVISFGCLFLTGAVLSCIWLLMKRRKERKLIRERKDIDVDEH 71
           P P  PSP ++S+T IVIV +SFG +F    + +C+   +K++K++K I+E + I VDEH
Sbjct: 50  PPPPAPSPSNNSTTTIVIVFVSFGGVFFLAFLAACLCCFLKKKKKKKEIKETELIHVDEH 109

Query: 72  LKVHEMIVRGPHGPKKVLVSVDDDVRVHEDVHRSE 107
            K+ E IV GPHGP+ +++ ++DDV V E + + E
Sbjct: 110 KKIKEAIVEGPHGPQAIVLEIEDDVHVDEVIKKEE 144

[Close]

BLAST of Spo18256.1 vs. NCBI nr
Match: gi|229002017|dbj|BAH57855.1| (tracheary element differentiation-related 7B [Zinnia violacea])

HSP 1 Score: 84.3 bits (207), Expect = 1.600e-13
Identity = 41/102 (40.20%), Postives = 69/102 (67.65%), Query Frame = 1


		  
Query: 14  PAPPSPDSSSSTVIVIVVISFGCLFLTGAVLSCIWLLMKRRKERKLIRERKDIDVDEHLK 73
           P PPSP S+ ST IV++ +S G +F     ++ +W  +K++K +K++R+ ++I  DEH K
Sbjct: 159 PPPPSP-SNHSTTIVVIFVSCGGVFFLAFAMAALWCFLKKKK-KKMVRKAENIHFDEHRK 218

Query: 74  VHEMIVRGPHGPKKVLVSVDDDVRVHEDVHRSEVDMVGKGKH 116
           V E I +GPHG +  ++SV+DD+ + ED+ +SE++   KG H
Sbjct: 219 VTERIEQGPHGTETAILSVEDDIHIEEDIKKSEIEDFRKGLH 258

[Close]

BLAST of Spo18256.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QDM4_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_198040 PE=3 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 5.200e-59
Identity = 119/119 (100.00%), Postives = 119/119 (100.00%), Query Frame = 1


		  
Query: 1   MASTDVNSSFHPHPAPPSPDSSSSTVIVIVVISFGCLFLTGAVLSCIWLLMKRRKERKLI 60
           MASTDVNSSFHPHPAPPSPDSSSSTVIVIVVISFGCLFLTGAVLSCIWLLMKRRKERKLI
Sbjct: 1   MASTDVNSSFHPHPAPPSPDSSSSTVIVIVVISFGCLFLTGAVLSCIWLLMKRRKERKLI 60

Query: 61  RERKDIDVDEHLKVHEMIVRGPHGPKKVLVSVDDDVRVHEDVHRSEVDMVGKGKHGEAS 120
           RERKDIDVDEHLKVHEMIVRGPHGPKKVLVSVDDDVRVHEDVHRSEVDMVGKGKHGEAS
Sbjct: 61  RERKDIDVDEHLKVHEMIVRGPHGPKKVLVSVDDDVRVHEDVHRSEVDMVGKGKHGEAS 119

[Close]

BLAST of Spo18256.1 vs. UniProtKB/TrEMBL
Match: A0A0J8FUT1_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_1g010760 PE=4 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 2.000e-34
Identity = 83/112 (74.11%), Postives = 91/112 (81.25%), Query Frame = 1


		  
Query: 11  HPHPA----PPSPDSSSSTVIVIVVISFGCLFLTGAVLSCIWLLM-KRRKERKLIRERKD 70
           HPHP     PPS D S  TVIVI+ ISFGCLFL GA+L CIW  M K+R ERKLI ERKD
Sbjct: 19  HPHPTPVPPPPSLD-SHPTVIVIIFISFGCLFLLGALLLCIWWFMKKKRTERKLINERKD 78

Query: 71  IDVDEHLKVHEMIVRGPHGPKKVLVSVDDDVRVHEDVHRSEVDMVGKGKHGE 118
           IDVDEHLKV EMI+ G HGPKKVLVSV+D++RVHEDVHRSEVDMVGKG HG+
Sbjct: 79  IDVDEHLKVQEMIMAGRHGPKKVLVSVEDEIRVHEDVHRSEVDMVGKGLHGK 129

[Close]

BLAST of Spo18256.1 vs. UniProtKB/TrEMBL
Match: A0A103XCW0_CYNCS (Uncharacterized protein OS=Cynara cardunculus var. scolymus GN=Ccrd_024163 PE=4 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 3.500e-15
Identity = 43/109 (39.45%), Postives = 74/109 (67.89%), Query Frame = 1


		  
Query: 12  PHPAPPSPDSSSSTVIVIVVISFGCLFLTGAVLSCIWLLMKRRKERKLIRERKDIDVDEH 71
           P P PPSP  + +T+IV+V +S G +F    +++ +W  +K++K  K++R+ +++ VDEH
Sbjct: 110 PPPPPPSP-GNHTTIIVVVFVSCGGVFFLAFIMAALWCFLKKKK--KMVRKAENVHVDEH 169

Query: 72  LKVHEMIVRGPHGPKKVLVSVDDDVRVHEDVHRSEVDMVGKGKHGEASV 121
             V E IV+GPHG + V++SV+DD+ + ED+ +SE++   KG H  + V
Sbjct: 170 RTVKESIVQGPHGTETVMLSVEDDIHIEEDIRKSEIENFRKGLHLNSGV 215

[Close]

BLAST of Spo18256.1 vs. UniProtKB/TrEMBL
Match: A0A067L3K0_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_25232 PE=4 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 3.000e-14
Identity = 41/95 (43.16%), Postives = 65/95 (68.42%), Query Frame = 1


		  
Query: 12  PHPAPPSPDSSSSTVIVIVVISFGCLFLTGAVLSCIWLLMKRRKERKLIRERKDIDVDEH 71
           P P  PSP ++S+T IVIV +SFG +F    + +C+   +K++K++K I+E + I VDEH
Sbjct: 50  PPPPAPSPSNNSTTTIVIVFVSFGGVFFLAFLAACLCCFLKKKKKKKEIKETELIHVDEH 109

Query: 72  LKVHEMIVRGPHGPKKVLVSVDDDVRVHEDVHRSE 107
            K+ E IV GPHGP+ +++ ++DDV V E + + E
Sbjct: 110 KKIKEAIVEGPHGPQAIVLEIEDDVHVDEVIKKEE 144

[Close]

BLAST of Spo18256.1 vs. UniProtKB/TrEMBL
Match: C1PGW2_ZINVI (Tracheary element differentiation-related 7B OS=Zinnia violacea GN=TED7B PE=2 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.100e-13
Identity = 41/102 (40.20%), Postives = 69/102 (67.65%), Query Frame = 1


		  
Query: 14  PAPPSPDSSSSTVIVIVVISFGCLFLTGAVLSCIWLLMKRRKERKLIRERKDIDVDEHLK 73
           P PPSP S+ ST IV++ +S G +F     ++ +W  +K++K +K++R+ ++I  DEH K
Sbjct: 159 PPPPSP-SNHSTTIVVIFVSCGGVFFLAFAMAALWCFLKKKK-KKMVRKAENIHFDEHRK 218

Query: 74  VHEMIVRGPHGPKKVLVSVDDDVRVHEDVHRSEVDMVGKGKH 116
           V E I +GPHG +  ++SV+DD+ + ED+ +SE++   KG H
Sbjct: 219 VTERIEQGPHGTETAILSVEDDIHIEEDIKKSEIEDFRKGLH 258

[Close]

BLAST of Spo18256.1 vs. TAIR (Arabidopsis)
Match: AT1G43790.1 (tracheary element differentiation-related 6)

HSP 1 Score: 79.3 bits (194), Expect = 1.800e-15
Identity = 49/122 (40.16%), Postives = 72/122 (59.02%), Query Frame = 1


		  
Query: 1   MASTDVNSSFHPHPAPPSPDSSSSTVIVIVVISFGCLFLTGAVLSCIWLLMKRRKERKLI 60
           MASTD  S + P P P       +TV+V+V +S GC+     +   IW L+K+R  +   
Sbjct: 1   MASTD--SVYRPTPTP----DHDTTVVVVVFVSLGCVMFLAFLAFVIWFLIKKRSRKH-- 60

Query: 61  RERKD-IDVDEHLKVHEMIVRGPHGPKKVLVSVDDDVRVHEDVHRSEVDMVGKGKHGEAS 120
           RER + + VDEH K+ E IV GP+G K V++SV+DDV++ + + R E D+   G  G + 
Sbjct: 61  RERSEAVRVDEHFKMKEAIVEGPNGQKSVVLSVEDDVKIEDAIKREEKDLKKDGGVGSSV 114

Query: 121 VS 122
           VS
Sbjct: 121 VS 114

[Close]

BLAST of Spo18256.1 vs. TAIR (Arabidopsis)
Match: AT5G48920.1 (tracheary element differentiation-related 7)

HSP 1 Score: 69.7 bits (169), Expect = 1.500e-12
Identity = 41/116 (35.34%), Postives = 64/116 (55.17%), Query Frame = 1


		  
Query: 11  HPHPAPPSP--------------------DSSSSTVIVIVVISFGCLFLTGAVLSCIWLL 70
           HPHP PPSP                     +    VI++VVIS G LF    + + ++  
Sbjct: 62  HPHPPPPSPYPHPHQPPPPPHVLPPPPPTPAPGHHVIIVVVISLGSLFFLAFLAAALFCY 121

Query: 71  MKRRKERKLIRERKDIDVDEHLKVHEMIVRGPHGPKKVLVSVDDDVRVHEDVHRSE 107
           +K+R  RK   + + I+ DEHLKV E IV+GPHG +  +V +++D+ + ED+H++E
Sbjct: 122 LKKR--RKSSTKAEIIEFDEHLKVQETIVQGPHGEQTRVVMLEEDIHLVEDIHKTE 175

The following BLAST results are available for this feature:

BLAST of Spo18256.1 vs. NCBI nr
BLAST of Spo18256.1 vs. UniProtKB/TrEMBL
BLAST of Spo18256.1 vs. ExPASy Swiss-Prot
BLAST of Spo18256.1 vs. TAIR (Arabidopsis)

BLAST of Spo18256.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902140135\|gb\|KNA04616.1\|	7.5e-59	100.	hypothetical protein SOVF_1980...	[more]
gi\|870868643\|gb\|KMT19446.1\|	2.9e-34	74.1	hypothetical protein BVRB_1g01...	[more]
gi\|976900015\|gb\|KVH88308.1\|	5.1e-15	39.4	hypothetical protein Ccrd_0241...	[more]
gi\|802559251\|ref\|XP_012066081.1\|	4.3e-14	43.1	PREDICTED: proline-rich recept...	[more]
gi\|229002017\|dbj\|BAH57855.1\|	1.6e-13	40.2	tracheary element differentiat...	[more]

BLAST of Spo18256.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9QDM4_SPIOL	5.2e-59	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8FUT1_BETVU	2.0e-34	74.1	Uncharacterized protein OS=Bet...	[more]
A0A103XCW0_CYNCS	3.5e-15	39.4	Uncharacterized protein OS=Cyn...	[more]
A0A067L3K0_JATCU	3.0e-14	43.1	Uncharacterized protein OS=Jat...	[more]
C1PGW2_ZINVI	1.1e-13	40.2	Tracheary element differentiat...	[more]

BLAST of Spo18256.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Spo18256.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 2


Match Name	E-value	Identity	Description
AT1G43790.1	1.8e-15	40.1	tracheary element differentiat...	[more]
AT5G48920.1	1.5e-12	35.3	tracheary element differentiat...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013320	Concanavalin A-like lectin/glucanase domain	GENE3D	2.60.120.200		coord: 11..68 score: 3.
None	No IPR available	PANTHER	PTHR35697	FAMILY NOT NAMED	coord: 12..109 score: 1.1
None	No IPR available	PANTHER	PTHR35697:SF1	SUBFAMILY NOT NAMED	coord: 12..109 score: 1.1

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042744	hydrogen peroxide catabolic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0006979	response to oxidative stress
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004601	peroxidase activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo12932	0.70	Barchart \| Table