Spo19397 (gene)

Overview
NameSpo19397
Typegene
OrganismSpinacia oleracea (Spinach)
DescriptionCysteine protease ATG4B (3.4.22.-) (Autophagy-related protein 4 homolog B)
LocationSpoScf_62496 : 62 .. 574 (-)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGAATTTGTTTGAGAGTACTGGTACTGCAAATTCATCTCCCGGTACTAGTTCCTCTTCAATTTCCCCTAATTTCAAACGCATTGCTTCTAATTCTTCCGATCACAAATCGTCATTATTGCCATCAACTAGTGCTTCGTCGTTGTTGTCTGGAATTGTTGTTTCCGCTTTCTCGATTTTTGAGCCTTGTGTGGAGGAGGGACTGGATACGGGTTCGAATTCTCGTGAGACGAAGGGGGTTGGAAATAAGACCAAGATTAGTAATTGTTGTAGTAGTAATGGTTGGGCGGCTGCTGTAAAGAGGGTTATGGCGAGTGGATCGATGAGGAGGATTAAGGAGCATATGTTCGGGTTTACTAAGTCTAGTGCTGTCACCTCTACAAGTGATATTTGGTTGTTGGGTGTTTGCTATAAACTTGATGATGCCATTGGTGGTGATCCTGTTAGTAGCAGCGGATTTGCTGCATTTGTCGAGGATTTTTCATCGAGGATTTTGATGACTTATAGGAAA

mRNA sequence

ATGAAGAATTTGTTTGAGAGTACTGGTACTGCAAATTCATCTCCCGGTACTAGTTCCTCTTCAATTTCCCCTAATTTCAAACGCATTGCTTCTAATTCTTCCGATCACAAATCGTCATTATTGCCATCAACTAGTGCTTCGTCGTTGTTGTCTGGAATTGTTGTTTCCGCTTTCTCGATTTTTGAGCCTTGTGTGGAGGAGGGACTGGATACGGGTTCGAATTCTCGTGAGACGAAGGGGGTTGGAAATAAGACCAAGATTAGTAATTGTTGTAGTAGTAATGGTTGGGCGGCTGCTGTAAAGAGGGTTATGGCGAGTGGATCGATGAGGAGGATTAAGGAGCATATGTTCGGGTTTACTAAGTCTAGTGCTGTCACCTCTACAAGTGATATTTGGTTGTTGGGTGTTTGCTATAAACTTGATGATGCCATTGGTGGTGATCCTGTTAGTAGCAGCGGATTTGCTGCATTTGTCGAGGATTTTTCATCGAGGATTTTGATGACTTATAGGAAA

Coding sequence (CDS)

ATGAAGAATTTGTTTGAGAGTACTGGTACTGCAAATTCATCTCCCGGTACTAGTTCCTCTTCAATTTCCCCTAATTTCAAACGCATTGCTTCTAATTCTTCCGATCACAAATCGTCATTATTGCCATCAACTAGTGCTTCGTCGTTGTTGTCTGGAATTGTTGTTTCCGCTTTCTCGATTTTTGAGCCTTGTGTGGAGGAGGGACTGGATACGGGTTCGAATTCTCGTGAGACGAAGGGGGTTGGAAATAAGACCAAGATTAGTAATTGTTGTAGTAGTAATGGTTGGGCGGCTGCTGTAAAGAGGGTTATGGCGAGTGGATCGATGAGGAGGATTAAGGAGCATATGTTCGGGTTTACTAAGTCTAGTGCTGTCACCTCTACAAGTGATATTTGGTTGTTGGGTGTTTGCTATAAACTTGATGATGCCATTGGTGGTGATCCTGTTAGTAGCAGCGGATTTGCTGCATTTGTCGAGGATTTTTCATCGAGGATTTTGATGACTTATAGGAAA

Protein sequence

MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSIFEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFTKSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSRILMTYRK
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo19397.1Spo19397.1mRNA


Homology
[Close]
BLAST of Spo19397.1 vs. NCBI nr
Match: gi|902151889|gb|KNA05568.1| (hypothetical protein SOVF_189160 isoform B [Spinacia oleracea])

HSP 1 Score: 326.6 bits (836), Expect = 2.500e-86
Identity = 171/171 (100.00%), Postives = 171/171 (100.00%), Query Frame = 1 

		  

Query: 1   MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSI 60
           MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSI
Sbjct: 1   MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSI 60

Query: 61  FEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFT 120
           FEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFT
Sbjct: 61  FEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFT 120

Query: 121 KSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSRILMTYRK 172
           KSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSRILMTYRK
Sbjct: 121 KSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSRILMTYRK 171
[Close]
BLAST of Spo19397.1 vs. NCBI nr
Match: gi|902151888|gb|KNA05567.1| (hypothetical protein SOVF_189160 isoform A [Spinacia oleracea])

HSP 1 Score: 326.6 bits (836), Expect = 2.500e-86
Identity = 171/171 (100.00%), Postives = 171/171 (100.00%), Query Frame = 1 

		  

Query: 1   MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSI 60
           MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSI
Sbjct: 1   MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSI 60

Query: 61  FEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFT 120
           FEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFT
Sbjct: 61  FEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFT 120

Query: 121 KSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSRILMTYRK 172
           KSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSRILMTYRK
Sbjct: 121 KSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSRILMTYRK 171
[Close]
BLAST of Spo19397.1 vs. NCBI nr
Match: gi|731323648|ref|XP_010672541.1| (PREDICTED: cysteine protease ATG4 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 237.3 bits (604), Expect = 2.000e-59
Identity = 135/174 (77.59%), Postives = 143/174 (82.18%), Query Frame = 1 

		  

Query: 1   MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSI 60
           MKNLFES GT NSS G +SS+ISP FK I SNSSDHKSS   ST ASSLLSGI VSAFSI
Sbjct: 1   MKNLFESAGTENSSSGATSSTISPKFKHICSNSSDHKSSS-SSTRASSLLSGIFVSAFSI 60

Query: 61  FEPCVEEGLDTGSNSR---ETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMF 120
           FE   EEG ++ SNSR   E KGVG K KISN  SSNGW  AVKR+MASGSMRRIKEHMF
Sbjct: 61  FEHRSEEGRNSSSNSRSSCEKKGVGAKNKISNS-SSNGWGTAVKRIMASGSMRRIKEHMF 120

Query: 121 GFTKSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSRILMTYRK 172
           GF +SS + STSDIWLLGVCYKLDDA GGDPVSSSGFAAFVEDFSSRIL+TYRK
Sbjct: 121 GFNRSSIIASTSDIWLLGVCYKLDDATGGDPVSSSGFAAFVEDFSSRILLTYRK 172
[Close]
BLAST of Spo19397.1 vs. NCBI nr
Match: gi|728828696|gb|KHG08139.1| (Cysteine protease ATG4 [Gossypium arboreum])

HSP 1 Score: 108.2 bits (269), Expect = 1.400e-20
Identity = 63/127 (49.61%), Postives = 78/127 (61.42%), Query Frame = 1 

		  

Query: 45  SASSLLSGIVVSAFSIFEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVM 104
           S +SL S    SAFS+F        DT S S  +     K+  S    +NGW AAVKRV+
Sbjct: 74  SKTSLWSNFFASAFSVF--------DTYSESSSSSACEKKSSFSK---TNGWTAAVKRVV 133

Query: 105 ASGSMRRIKEHMFGFTKSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSR 164
           + GSMRRI E + G +K    +STSDIWLLG+CYK+     GD  ++S  AAF +DFSSR
Sbjct: 134 SGGSMRRIHERVLGPSKIGISSSTSDIWLLGLCYKISQESSGDVDATSALAAFKQDFSSR 189

Query: 165 ILMTYRK 172
           ILMTYRK
Sbjct: 194 ILMTYRK 189
[Close]
BLAST of Spo19397.1 vs. NCBI nr
Match: gi|568849538|ref|XP_006478507.1| (PREDICTED: cysteine protease ATG4 isoform X1 [Citrus sinensis])

HSP 1 Score: 104.0 bits (258), Expect = 2.700e-19
Identity = 74/177 (41.81%), Postives = 103/177 (58.19%), Query Frame = 1 

		  

Query: 1   MKNLFESTGTAN----SSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVS 60
           MK   E  G +     S+P T + S++     + S+ S         +S  SLLS +  S
Sbjct: 1   MKGFREKAGASKCFSKSTPDTPNRSLASVGSELGSSES--------KSSKGSLLSSLFNS 60

Query: 61  AFSIFEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHM 120
           AFS+FE   E      S++ E K V NK        SNGW AAVKR++ +GSMRRI E +
Sbjct: 61  AFSVFETYSE------SSANEKKAVHNK--------SNGWTAAVKRLVTAGSMRRIHERV 120

Query: 121 FGFTKSSAVTSTSDIWLLGVCYKL--DDAIGGDPVSSSGFAAFVEDFSSRILMTYRK 172
            G +++   +STSDIWLLGVC+K+  D+A+ GD   ++G A F +DFSSRIL++YRK
Sbjct: 121 LGPSRTGISSSTSDIWLLGVCHKIAQDEAL-GDAAGNNGLAEFNQDFSSRILISYRK 154
[Close]
BLAST of Spo19397.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QE75_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_189160 PE=3 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 1.800e-86
Identity = 171/171 (100.00%), Postives = 171/171 (100.00%), Query Frame = 1 

		  

Query: 1   MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSI 60
           MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSI
Sbjct: 1   MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSI 60

Query: 61  FEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFT 120
           FEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFT
Sbjct: 61  FEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFT 120

Query: 121 KSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSRILMTYRK 172
           KSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSRILMTYRK
Sbjct: 121 KSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSRILMTYRK 171
[Close]
BLAST of Spo19397.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QFV1_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_189160 PE=3 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 1.800e-86
Identity = 171/171 (100.00%), Postives = 171/171 (100.00%), Query Frame = 1 

		  

Query: 1   MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSI 60
           MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSI
Sbjct: 1   MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSI 60

Query: 61  FEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFT 120
           FEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFT
Sbjct: 61  FEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFT 120

Query: 121 KSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSRILMTYRK 172
           KSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSRILMTYRK
Sbjct: 121 KSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSRILMTYRK 171
[Close]
BLAST of Spo19397.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CPD3_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g059550 PE=3 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 1.400e-59
Identity = 135/174 (77.59%), Postives = 143/174 (82.18%), Query Frame = 1 

		  

Query: 1   MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSI 60
           MKNLFES GT NSS G +SS+ISP FK I SNSSDHKSS   ST ASSLLSGI VSAFSI
Sbjct: 1   MKNLFESAGTENSSSGATSSTISPKFKHICSNSSDHKSSS-SSTRASSLLSGIFVSAFSI 60

Query: 61  FEPCVEEGLDTGSNSR---ETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMF 120
           FE   EEG ++ SNSR   E KGVG K KISN  SSNGW  AVKR+MASGSMRRIKEHMF
Sbjct: 61  FEHRSEEGRNSSSNSRSSCEKKGVGAKNKISNS-SSNGWGTAVKRIMASGSMRRIKEHMF 120

Query: 121 GFTKSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSRILMTYRK 172
           GF +SS + STSDIWLLGVCYKLDDA GGDPVSSSGFAAFVEDFSSRIL+TYRK
Sbjct: 121 GFNRSSIIASTSDIWLLGVCYKLDDATGGDPVSSSGFAAFVEDFSSRILLTYRK 172
[Close]
BLAST of Spo19397.1 vs. UniProtKB/TrEMBL
Match: A0A0B0N8F2_GOSAR (Cysteine protease ATG4 OS=Gossypium arboreum GN=F383_05577 PE=3 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 9.900e-21
Identity = 63/127 (49.61%), Postives = 78/127 (61.42%), Query Frame = 1 

		  

Query: 45  SASSLLSGIVVSAFSIFEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVM 104
           S +SL S    SAFS+F        DT S S  +     K+  S    +NGW AAVKRV+
Sbjct: 74  SKTSLWSNFFASAFSVF--------DTYSESSSSSACEKKSSFSK---TNGWTAAVKRVV 133

Query: 105 ASGSMRRIKEHMFGFTKSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSR 164
           + GSMRRI E + G +K    +STSDIWLLG+CYK+     GD  ++S  AAF +DFSSR
Sbjct: 134 SGGSMRRIHERVLGPSKIGISSSTSDIWLLGLCYKISQESSGDVDATSALAAFKQDFSSR 189

Query: 165 ILMTYRK 172
           ILMTYRK
Sbjct: 194 ILMTYRK 189
[Close]
BLAST of Spo19397.1 vs. UniProtKB/TrEMBL
Match: A0A067DBF9_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g011418mg PE=3 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 2.400e-19
Identity = 74/177 (41.81%), Postives = 103/177 (58.19%), Query Frame = 1 

		  

Query: 1   MKNLFESTGTAN----SSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVS 60
           MK   E  G +     S+P T + S++     + S+ S         +S  SLLS +  S
Sbjct: 1   MKGFREKAGASKCFSKSTPDTPNRSLASVGSELGSSES--------KSSKGSLLSSLFNS 60

Query: 61  AFSIFEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHM 120
           AFS+FE   E      S++ E K V NK        SNGW AAVKR++ +GSMRRI E +
Sbjct: 61  AFSVFETYSE------SSASEKKAVHNK--------SNGWTAAVKRLVTAGSMRRIHERV 120

Query: 121 FGFTKSSAVTSTSDIWLLGVCYKL--DDAIGGDPVSSSGFAAFVEDFSSRILMTYRK 172
            G +++   +STSDIWLLGVC+K+  D+A+ GD   ++G A F +DFSSRIL++YRK
Sbjct: 121 LGPSRTGISSSTSDIWLLGVCHKIAQDEAL-GDAAGNNGLAEFNQDFSSRILISYRK 154
[Close]
BLAST of Spo19397.1 vs. ExPASy Swiss-Prot
Match: ATG4A_ARATH (Cysteine protease ATG4a OS=Arabidopsis thaliana GN=ATG4A PE=2 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 4.000e-15
Identity = 60/151 (39.74%), Postives = 82/151 (54.30%), Query Frame = 1 

		  

Query: 30  ASNSSDHKSSLLPSTSASS-------LLSGIVVSAFSIFEPCVEEGLDTGSNSRETKGVG 89
           +S S  H  S L S S  S       L S +  S+ S+ +P            RE+   G
Sbjct: 16  SSKSDTHDKSPLVSDSGPSDNKSKFTLWSNVFTSSSSVSQPY-----------RESSTSG 75

Query: 90  NKTKISNCCSSNGWAAAVKRV-MASGSMRRIKEHMFGFTKSSAVTSTSDIWLLGVCYKLD 149
           +K     C + NGW A VKRV MASG++RR +E + G  ++   ++TSD+WLLGVCYK+ 
Sbjct: 76  HKQV---CTTRNGWTAFVKRVSMASGAIRRFQERVLGPNRTGLPSTTSDVWLLGVCYKIS 135

Query: 150 -DAIGGDPVSSSGFAAFVEDFSSRILMTYRK 172
            D   G+  + +  AA   DFSS+ILMTYRK
Sbjct: 136 ADENSGETDTGTVLAALQLDFSSKILMTYRK 152
[Close]
BLAST of Spo19397.1 vs. ExPASy Swiss-Prot
Match: ATG4_MEDTR (Cysteine protease ATG4 OS=Medicago truncatula GN=ATG4 PE=3 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 2.600e-14
Identity = 65/172 (37.79%), Postives = 93/172 (54.07%), Query Frame = 1 

		  

Query: 1   MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSI 60
           +K+L +    A  S   SS+ I  N +  AS+ +    S  P    +SL S    S FS+
Sbjct: 3   LKDLCDRIVAAKCS-SKSSTEIVDNTQVPASSKAGSSDSKFPK---ASLWSTFFTSGFSV 62

Query: 61  FEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFT 120
            E   E      S+S E K V ++        ++GWAAAV++V++ GSMRR +E + G  
Sbjct: 63  DETYSE------SSSSEKKTVHSR--------NSGWAAAVRKVVSGGSMRRFQERVLGSC 122

Query: 121 KSSAVTSTSDIWLLGVCYKLDD-AIGGDPVSSSGFAAFVEDFSSRILMTYRK 172
           ++   +S  DIWLLGVC+K+      GD    + FAAF +DF SRIL+TYRK
Sbjct: 123 RTDVSSSDGDIWLLGVCHKISQHESTGDVDIRNVFAAFEQDFFSRILITYRK 156
[Close]
BLAST of Spo19397.1 vs. ExPASy Swiss-Prot
Match: ATG4B_ORYSJ (Cysteine protease ATG4B OS=Oryza sativa subsp. japonica GN=ATG4B PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.100e-11
Identity = 60/160 (37.50%), Postives = 82/160 (51.25%), Query Frame = 1 

		  

Query: 16  GTSSSSISPNFK-RIA--SNSSDHKSSLLPSTSASSLLSGIVVSAFSIFEPCVEEGLDTG 75
           G SSSS  P  +  IA  S+SS+ K       S +S+LS +  S F+IFE       D+ 
Sbjct: 8   GVSSSSSDPLCEGNIAPCSSSSEQKEDCSLKQSKTSILSCVFNSPFNIFEA----HQDSS 67

Query: 76  SNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFTKSSAVTSTSDIW 135
           +N       G          S  W+  ++R++ SGSM R       F  +S V ++SD+W
Sbjct: 68  ANKSPKSSSG----------SYDWSRVLRRIVCSGSMWR-------FLGTSKVLTSSDVW 127

Query: 136 LLGVCYKLD-DAIGGDPVSSSGFAAFVEDFSSRILMTYRK 172
            LG CYKL  +    D  S SG A F+EDFSSRI +TYR+
Sbjct: 128 FLGKCYKLSSEESSSDSDSESGHATFLEDFSSRIWITYRR 146
[Close]
BLAST of Spo19397.1 vs. ExPASy Swiss-Prot
Match: ATG4B_ORYSI (Cysteine protease ATG4B OS=Oryza sativa subsp. indica GN=ATG4B PE=1 SV=2)

HSP 1 Score: 69.7 bits (169), Expect = 3.500e-11
Identity = 60/160 (37.50%), Postives = 81/160 (50.62%), Query Frame = 1 

		  

Query: 16  GTSSSSISPNFK-RIA--SNSSDHKSSLLPSTSASSLLSGIVVSAFSIFEPCVEEGLDTG 75
           G SSSS  P  +  IA  S+SS+ K       S +S+LS +  S F+IFE       D+ 
Sbjct: 8   GVSSSSSDPLCEGNIAPCSSSSEQKEDCSLKQSKTSILSCVFNSPFNIFEA----HQDSS 67

Query: 76  SNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFTKSSAVTSTSDIW 135
           +N       G          S  W   ++R++ SGSM R       F  +S V ++SD+W
Sbjct: 68  ANKSPKSSSG----------SYDWLRVLRRIVCSGSMWR-------FLGTSKVLTSSDVW 127

Query: 136 LLGVCYKLD-DAIGGDPVSSSGFAAFVEDFSSRILMTYRK 172
            LG CYKL  +    D  S SG A F+EDFSSRI +TYR+
Sbjct: 128 FLGKCYKLSSEESSSDSDSESGHATFLEDFSSRIWITYRR 146
[Close]
BLAST of Spo19397.1 vs. ExPASy Swiss-Prot
Match: ATG4B_ARATH (Cysteine protease ATG4b OS=Arabidopsis thaliana GN=ATG4B PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.300e-10
Identity = 55/169 (32.54%), Postives = 85/169 (50.30%), Query Frame = 1 

		  

Query: 5   FESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSIFEPC 64
           F  +  ++SS        SP      S SSD+KS+L       +L S +V S+  + + C
Sbjct: 8   FVPSKCSSSSTSEKRDISSPTSLVSDSASSDNKSNL-------TLCSDVVASSSPVSQLC 67

Query: 65  VEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRV-MASGSMRRIKEHMFGFTKSS 124
             E   +G N               C + + W   +K   MASG++RR ++ + G +++ 
Sbjct: 68  -REASTSGHNPV-------------CTTHSSWTVILKTASMASGAIRRFQDRVLGPSRTG 127

Query: 125 AVTSTSDIWLLGVCYKLDDAIGGDPVSSSG-FAAFVEDFSSRILMTYRK 172
             +STS+IWLLGVCYK+ +    +   +    AAF +DFSS ILMTYR+
Sbjct: 128 ISSSTSEIWLLGVCYKISEGESSEEADAGRVLAAFRQDFSSLILMTYRR 155
[Close]
BLAST of Spo19397.1 vs. TAIR (Arabidopsis)
Match: AT2G44140.1 (Peptidase family C54 protein)

HSP 1 Score: 82.8 bits (203), Expect = 2.200e-16
Identity = 60/151 (39.74%), Postives = 82/151 (54.30%), Query Frame = 1 

		  

Query: 30  ASNSSDHKSSLLPSTSASS-------LLSGIVVSAFSIFEPCVEEGLDTGSNSRETKGVG 89
           +S S  H  S L S S  S       L S +  S+ S+ +P            RE+   G
Sbjct: 16  SSKSDTHDKSPLVSDSGPSDNKSKFTLWSNVFTSSSSVSQPY-----------RESSTSG 75

Query: 90  NKTKISNCCSSNGWAAAVKRV-MASGSMRRIKEHMFGFTKSSAVTSTSDIWLLGVCYKLD 149
           +K     C + NGW A VKRV MASG++RR +E + G  ++   ++TSD+WLLGVCYK+ 
Sbjct: 76  HKQV---CTTRNGWTAFVKRVSMASGAIRRFQERVLGPNRTGLPSTTSDVWLLGVCYKIS 135

Query: 150 -DAIGGDPVSSSGFAAFVEDFSSRILMTYRK 172
            D   G+  + +  AA   DFSS+ILMTYRK
Sbjct: 136 ADENSGETDTGTVLAALQLDFSSKILMTYRK 152
[Close]
BLAST of Spo19397.1 vs. TAIR (Arabidopsis)
Match: AT3G59950.1 (Peptidase family C54 protein)

HSP 1 Score: 67.8 bits (164), Expect = 7.500e-12
Identity = 55/169 (32.54%), Postives = 85/169 (50.30%), Query Frame = 1 

		  

Query: 5   FESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSIFEPC 64
           F  +  ++SS        SP      S SSD+KS+L       +L S +V S+  + + C
Sbjct: 8   FVPSKCSSSSTSEKRDISSPTSLVSDSASSDNKSNL-------TLCSDVVASSSPVSQLC 67

Query: 65  VEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRV-MASGSMRRIKEHMFGFTKSS 124
             E   +G N               C + + W   +K   MASG++RR ++ + G +++ 
Sbjct: 68  -REASTSGHNPV-------------CTTHSSWTVILKTASMASGAIRRFQDRVLGPSRTG 127

Query: 125 AVTSTSDIWLLGVCYKLDDAIGGDPVSSSG-FAAFVEDFSSRILMTYRK 172
             +STS+IWLLGVCYK+ +    +   +    AAF +DFSS ILMTYR+
Sbjct: 128 ISSSTSEIWLLGVCYKISEGESSEEADAGRVLAAFRQDFSSLILMTYRR 155
The following BLAST results are available for this feature:
BLAST of Spo19397.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902151889|gb|KNA05568.1|2.5e-86100.hypothetical protein SOVF_1891... [more]
gi|902151888|gb|KNA05567.1|2.5e-86100.hypothetical protein SOVF_1891... [more]
gi|731323648|ref|XP_010672541.1|2.0e-5977.5PREDICTED: cysteine protease A... [more]
gi|728828696|gb|KHG08139.1|1.4e-2049.6Cysteine protease ATG4 [Gossyp... [more]
gi|568849538|ref|XP_006478507.1|2.7e-1941.8PREDICTED: cysteine protease A... [more]
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BLAST of Spo19397.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9QE75_SPIOL1.8e-86100.Uncharacterized protein OS=Spi... [more]
A0A0K9QFV1_SPIOL1.8e-86100.Uncharacterized protein OS=Spi... [more]
A0A0J8CPD3_BETVU1.4e-5977.5Uncharacterized protein OS=Bet... [more]
A0A0B0N8F2_GOSAR9.9e-2149.6Cysteine protease ATG4 OS=Goss... [more]
A0A067DBF9_CITSI2.4e-1941.8Uncharacterized protein OS=Cit... [more]
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BLAST of Spo19397.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5
Match NameE-valueIdentityDescription
ATG4A_ARATH4.0e-1539.7Cysteine protease ATG4a OS=Ara... [more]
ATG4_MEDTR2.6e-1437.7Cysteine protease ATG4 OS=Medi... [more]
ATG4B_ORYSJ2.1e-1137.5Cysteine protease ATG4B OS=Ory... [more]
ATG4B_ORYSI3.5e-1137.5Cysteine protease ATG4B OS=Ory... [more]
ATG4B_ARATH1.3e-1032.5Cysteine protease ATG4b OS=Ara... [more]
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BLAST of Spo19397.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 2
Match NameE-valueIdentityDescription
AT2G44140.12.2e-1639.7Peptidase family C54 protein[more]
AT3G59950.17.5e-1232.5Peptidase family C54 protein[more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005078Peptidase C54PANTHERPTHR22624APG4 AUTOPHAGY 4-RELATEDcoord: 13..171
score: 2.3
NoneNo IPR availablePANTHERPTHR22624:SF34AUTOPHAGY-SPECIFIC GENE 4, ISOFORM Acoord: 13..171
score: 2.3

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050999 regulation of nitric-oxide synthase activity
biological_process GO:0010162 seed dormancy process
biological_process GO:0010267 production of ta-siRNAs involved in RNA interference
biological_process GO:0016567 protein ubiquitination
biological_process GO:0007131 reciprocal meiotic recombination
biological_process GO:0033044 regulation of chromosome organization
biological_process GO:0009909 regulation of flower development
biological_process GO:0050826 response to freezing
biological_process GO:0009845 seed germination
biological_process GO:0010638 positive regulation of organelle organization
biological_process GO:0010182 sugar mediated signaling pathway
biological_process GO:0010090 trichome morphogenesis
biological_process GO:0010050 vegetative phase change
biological_process GO:0009616 virus induced gene silencing
biological_process GO:0052542 defense response by callose deposition
biological_process GO:0050832 defense response to fungus
biological_process GO:0009867 jasmonic acid mediated signaling pathway
biological_process GO:0035196 production of miRNAs involved in gene silencing by miRNA
biological_process GO:0048235 pollen sperm cell differentiation
biological_process GO:0031348 negative regulation of defense response
biological_process GO:0051567 histone H3-K9 methylation
biological_process GO:0031048 chromatin silencing by small RNA
biological_process GO:0009855 determination of bilateral symmetry
biological_process GO:0006306 DNA methylation
biological_process GO:0006281 DNA repair
biological_process GO:0009793 embryo development ending in seed dormancy
biological_process GO:0009560 embryo sac egg cell differentiation
biological_process GO:0016572 histone phosphorylation
biological_process GO:0009640 photomorphogenesis
biological_process GO:0019915 lipid storage
biological_process GO:0048574 long-day photoperiodism, flowering
biological_process GO:0010014 meristem initiation
biological_process GO:0010073 meristem maintenance
biological_process GO:0006346 methylation-dependent chromatin silencing
biological_process GO:0006312 mitotic recombination
biological_process GO:0043547 positive regulation of GTPase activity
biological_process GO:0000165 MAPK cascade
biological_process GO:0043069 negative regulation of programmed cell death
biological_process GO:0007267 cell-cell signaling
biological_process GO:0006465 signal peptide processing
biological_process GO:0030244 cellulose biosynthetic process
biological_process GO:0071555 cell wall organization
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0006011 UDP-glucose metabolic process
biological_process GO:0008643 carbohydrate transport
biological_process GO:0048193 Golgi vesicle transport
biological_process GO:0002098 tRNA wobble uridine modification
biological_process GO:0006623 protein targeting to vacuole
biological_process GO:0006614 SRP-dependent cotranslational protein targeting to membrane
biological_process GO:0009932 cell tip growth
biological_process GO:0006635 fatty acid beta-oxidation
biological_process GO:0042967 obsolete acyl-carrier-protein biosynthetic process
biological_process GO:0016558 protein import into peroxisome matrix
biological_process GO:0030968 endoplasmic reticulum unfolded protein response
biological_process GO:0006887 exocytosis
biological_process GO:0034227 tRNA thio-modification
biological_process GO:0006612 protein targeting to membrane
biological_process GO:0006508 proteolysis
biological_process GO:0010310 regulation of hydrogen peroxide metabolic process
biological_process GO:0010363 regulation of plant-type hypersensitive response
biological_process GO:0010337 regulation of salicylic acid metabolic process
biological_process GO:0002679 respiratory burst involved in defense response
biological_process GO:0010200 response to chitin
biological_process GO:0009862 systemic acquired resistance, salicylic acid mediated signaling pathway
biological_process GO:0016571 histone methylation
biological_process GO:0009410 response to xenobiotic stimulus
biological_process GO:0045893 positive regulation of transcription, DNA-templated
biological_process GO:0043687 post-translational protein modification
biological_process GO:0000338 protein deneddylation
biological_process GO:0016579 protein deubiquitination
biological_process GO:0032447 protein urmylation
biological_process GO:0080157 regulation of plant-type cell wall organization or biogenesis
biological_process GO:0000302 response to reactive oxygen species
biological_process GO:0006338 chromatin remodeling
biological_process GO:0000398 mRNA splicing, via spliceosome
biological_process GO:0006810 transport
biological_process GO:0042742 defense response to bacterium
biological_process GO:0008152 metabolic process
biological_process GO:0009088 threonine biosynthetic process
biological_process GO:0007165 signal transduction
biological_process GO:0006396 RNA processing
biological_process GO:0009934 regulation of meristem structural organization
biological_process GO:0006470 protein dephosphorylation
biological_process GO:0000278 mitotic cell cycle
biological_process GO:0015672 monovalent inorganic cation transport
biological_process GO:0055085 transmembrane transport
biological_process GO:0016255 attachment of GPI anchor to protein
biological_process GO:0006816 calcium ion transport
biological_process GO:0009630 gravitropism
biological_process GO:0006486 protein glycosylation
biological_process GO:0015991 ATP hydrolysis coupled proton transport
biological_process GO:0030001 metal ion transport
biological_process GO:0007186 G-protein coupled receptor signaling pathway
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
biological_process GO:0016310 phosphorylation
biological_process GO:0031047 gene silencing by RNA
biological_process GO:0009089 lysine biosynthetic process via diaminopimelate
biological_process GO:0006544 glycine metabolic process
biological_process GO:0042176 regulation of protein catabolic process
biological_process GO:0050790 regulation of catalytic activity
biological_process GO:0009069 serine family amino acid metabolic process
biological_process GO:0006468 protein phosphorylation
biological_process GO:0006446 regulation of translational initiation
biological_process GO:0034314 Arp2/3 complex-mediated actin nucleation
biological_process GO:0006563 L-serine metabolic process
biological_process GO:1902600 proton transmembrane transport
biological_process GO:0006885 regulation of pH
biological_process GO:0006857 oligopeptide transport
cellular_component GO:0005785 signal recognition particle receptor complex
cellular_component GO:0005768 endosome
cellular_component GO:0005786 signal recognition particle, endoplasmic reticulum targeting
cellular_component GO:0005774 vacuolar membrane
cellular_component GO:0042765 GPI-anchor transamidase complex
cellular_component GO:0000145 exocyst
cellular_component GO:0005801 cis-Golgi network
cellular_component GO:0031410 cytoplasmic vesicle
cellular_component GO:0009570 chloroplast stroma
cellular_component GO:0017177 glucosidase II complex
cellular_component GO:0005829 cytosol
cellular_component GO:0005834 heterotrimeric G-protein complex
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009524 phragmoplast
cellular_component GO:0033179 proton-transporting V-type ATPase, V0 domain
cellular_component GO:0005797 Golgi medial cisterna
cellular_component GO:0009941 chloroplast envelope
cellular_component GO:0005856 cytoskeleton
cellular_component GO:0000502 proteasome complex
cellular_component GO:0005885 Arp2/3 protein complex
cellular_component GO:0005840 ribosome
cellular_component GO:0009507 chloroplast
cellular_component GO:0000159 protein phosphatase type 2A complex
cellular_component GO:0016020 membrane
cellular_component GO:0000139 Golgi membrane
cellular_component GO:0030659 cytoplasmic vesicle membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0008287 protein serine/threonine phosphatase complex
cellular_component GO:0031011 Ino80 complex
cellular_component GO:0019773 proteasome core complex, alpha-subunit complex
cellular_component GO:0035267 NuA4 histone acetyltransferase complex
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005730 nucleolus
cellular_component GO:0000812 Swr1 complex
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0005634 nucleus
cellular_component GO:0005802 trans-Golgi network
molecular_function GO:0005525 GTP binding
molecular_function GO:0004842 ubiquitin-protein transferase activity
molecular_function GO:0005096 GTPase activator activity
molecular_function GO:0033926 glycopeptide alpha-N-acetylgalactosaminidase activity
molecular_function GO:0019706 protein-cysteine S-palmitoyltransferase activity
molecular_function GO:0019888 protein phosphatase regulator activity
molecular_function GO:0003950 NAD+ ADP-ribosyltransferase activity
molecular_function GO:0000035 acyl binding
molecular_function GO:0004575 sucrose alpha-glucosidase activity
molecular_function GO:0003924 GTPase activity
molecular_function GO:0016757 transferase activity, transferring glycosyl groups
molecular_function GO:0030246 carbohydrate binding
molecular_function GO:0005047 signal recognition particle binding
molecular_function GO:0005488 binding
molecular_function GO:0016760 cellulose synthase (UDP-forming) activity
molecular_function GO:0005200 structural constituent of cytoskeleton
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0003743 translation initiation factor activity
molecular_function GO:0035673 oligopeptide transmembrane transporter activity
molecular_function GO:0015299 solute:proton antiporter activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0016746 transferase activity, transferring acyl groups
molecular_function GO:0004722 protein serine/threonine phosphatase activity
molecular_function GO:0019901 protein kinase binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0042393 histone binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0004298 threonine-type endopeptidase activity
molecular_function GO:0004871 obsolete signal transducer activity
molecular_function GO:0008233 peptidase activity
molecular_function GO:0042626 ATPase activity, coupled to transmembrane movement of substances
molecular_function GO:0005515 protein binding
molecular_function GO:0015078 proton transmembrane transporter activity
molecular_function GO:0008234 cysteine-type peptidase activity
molecular_function GO:0036459 thiol-dependent ubiquitinyl hydrolase activity
molecular_function GO:0030234 enzyme regulator activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0004072 aspartate kinase activity
molecular_function GO:0016597 amino acid binding
molecular_function GO:0016779 nucleotidyltransferase activity
molecular_function GO:0000049 tRNA binding
molecular_function GO:0016787 hydrolase activity
RNA-Seq Expression
   



Co-expression
Gener valueExpression
Spo027310.83Barchart | Table
Spo267450.76Barchart | Table
Spo209570.73Barchart | Table
Spo173290.72Barchart | Table
Spo235270.71Barchart | Table
Spo059280.71Barchart | Table
Spo170320.71Barchart | Table
Spo021150.70Barchart | Table
Spo129420.70Barchart | Table
Spo197010.70Barchart | Table
Spo236640.70Barchart | Table
Spo008120.69Barchart | Table
Spo018010.69Barchart | Table
Spo027470.69Barchart | Table
Spo062410.69Barchart | Table
Spo069620.69Barchart | Table
Spo109600.69Barchart | Table
Spo140080.69Barchart | Table
Spo154200.69Barchart | Table
Spo184510.69Barchart | Table
Spo233460.69Barchart | Table
Spo033060.68Barchart | Table
Spo126630.68Barchart | Table
Spo224420.68Barchart | Table
Spo137060.68Barchart | Table
Spo035960.68Barchart | Table
Spo216910.68Barchart | Table
Spo031970.68Barchart | Table
Spo071990.68Barchart | Table
Spo057810.68Barchart | Table
Spo208260.68Barchart | Table
Spo207040.68Barchart | Table
Spo008670.67Barchart | Table
Spo083270.67Barchart | Table
Spo099820.67Barchart | Table
Spo029620.67Barchart | Table
Spo048650.67Barchart | Table
Spo146610.67Barchart | Table
Spo170210.67Barchart | Table
Spo173710.67Barchart | Table
Spo173910.67Barchart | Table
Spo190890.67Barchart | Table
Spo200650.67Barchart | Table
Spo228180.67Barchart | Table
Spo229060.67Barchart | Table
Spo231230.67Barchart | Table
Spo054130.67Barchart | Table
Spo233290.67Barchart | Table
Spo144410.66Barchart | Table
Spo085400.66Barchart | Table
Spo152700.66Barchart | Table
Spo113490.66Barchart | Table
Spo055570.66Barchart | Table
Spo085310.66Barchart | Table
Spo013970.66Barchart | Table
Spo035900.66Barchart | Table
Spo123680.66Barchart | Table
Spo206960.66Barchart | Table
Spo068050.66Barchart | Table
Spo049230.65Barchart | Table
Spo173860.65Barchart | Table
Spo092660.65Barchart | Table
Spo265600.65Barchart | Table
Spo185750.65Barchart | Table
Spo066790.65Barchart | Table
Spo195580.65Barchart | Table
Spo200710.65Barchart | Table
Spo110270.65Barchart | Table
Spo099490.65Barchart | Table
Spo182690.65Barchart | Table
Spo240660.65Barchart | Table
Spo163300.65Barchart | Table