Spo19759 (gene) | SpinachBase

Overview


Name	Spo19759
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	4-hydroxy-4-methyl-2-oxoglutarate aldolase (HMG aldolase) (4.1.1.3) (4.1.3.17) (Oxaloacetate decarboxylase)
Location	chr5 : 17648569 .. 17649072 (+)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTAGCAACTTCAGACATTTGTGATGCAAACTCAAGCCTCCTCTCAACAGGAGAACTAAAAGTGTTACTCCCTCTGTTCAAACCTTACGGCCGACGACAGGCCTTTAGCGGGCCTGTCGTTACGGTGAAGGTTTTCGAGGACAACGTTTTGGTCCGGGCTTTATTGGAGTCCAAAGACCACCAAGGAAAGGTCTTGGTGGTGGATGGTGGAGGAAGCATAAGGTGTGCTCTTTTGGGTGGGAATTTGGGCCAGATAGCCCATGATAATGGTTGGGCTGGAATAGTTGTGAATGGATGTGTAAGAGATGTGGATGAGATTAATGGTTGTGATATTGGAGTTTGGGCTTTAGGGACTAATCCAGTTAAGTCTAAAAAGAAGGGTTTAGGAGATAAACATGTTTCTGTTAATTTTGCTGGATCTTTGATTAATGATGGTGATTGGTTGTGGGCTGATAATGATGGTATTTTGATCTCCAAGCATCAACTCTCTGCCACTTCTTAA

mRNA sequence

ATGGTAGCAACTTCAGACATTTGTGATGCAAACTCAAGCCTCCTCTCAACAGGAGAACTAAAAGTGTTACTCCCTCTGTTCAAACCTTACGGCCGACGACAGGCCTTTAGCGGGCCTGTCGTTACGGTGAAGGTTTTCGAGGACAACGTTTTGGTCCGGGCTTTATTGGAGTCCAAAGACCACCAAGGAAAGGTCTTGGTGGTGGATGGTGGAGGAAGCATAAGGTGTGCTCTTTTGGGTGGGAATTTGGGCCAGATAGCCCATGATAATGGTTGGGCTGGAATAGTTGTGAATGGATGTGTAAGAGATGTGGATGAGATTAATGGTTGTGATATTGGAGTTTGGGCTTTAGGGACTAATCCAGTTAAGTCTAAAAAGAAGGGTTTAGGAGATAAACATGTTTCTGTTAATTTTGCTGGATCTTTGATTAATGATGGTGATTGGTTGTGGGCTGATAATGATGGTATTTTGATCTCCAAGCATCAACTCTCTGCCACTTCTTAA

Coding sequence (CDS)

ATGGTAGCAACTTCAGACATTTGTGATGCAAACTCAAGCCTCCTCTCAACAGGAGAACTAAAAGTGTTACTCCCTCTGTTCAAACCTTACGGCCGACGACAGGCCTTTAGCGGGCCTGTCGTTACGGTGAAGGTTTTCGAGGACAACGTTTTGGTCCGGGCTTTATTGGAGTCCAAAGACCACCAAGGAAAGGTCTTGGTGGTGGATGGTGGAGGAAGCATAAGGTGTGCTCTTTTGGGTGGGAATTTGGGCCAGATAGCCCATGATAATGGTTGGGCTGGAATAGTTGTGAATGGATGTGTAAGAGATGTGGATGAGATTAATGGTTGTGATATTGGAGTTTGGGCTTTAGGGACTAATCCAGTTAAGTCTAAAAAGAAGGGTTTAGGAGATAAACATGTTTCTGTTAATTTTGCTGGATCTTTGATTAATGATGGTGATTGGTTGTGGGCTGATAATGATGGTATTTTGATCTCCAAGCATCAACTCTCTGCCACTTCTTAA

Protein sequence

MVATSDICDANSSLLSTGELKVLLPLFKPYGRRQAFSGPVVTVKVFEDNVLVRALLESKDHQGKVLVVDGGGSIRCALLGGNLGQIAHDNGWAGIVVNGCVRDVDEINGCDIGVWALGTNPVKSKKKGLGDKHVSVNFAGSLINDGDWLWADNDGILISKHQLSATS

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo19759.1	Spo19759.1	mRNA

Homology

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BLAST of Spo19759.1 vs. NCBI nr
Match: gi|902177580|gb|KNA08974.1| (hypothetical protein SOVF_157860 [Spinacia oleracea])

HSP 1 Score: 345.1 bits (884), Expect = 6.700e-92
Identity = 167/167 (100.00%), Postives = 167/167 (100.00%), Query Frame = 1


		  
Query: 1   MVATSDICDANSSLLSTGELKVLLPLFKPYGRRQAFSGPVVTVKVFEDNVLVRALLESKD 60
           MVATSDICDANSSLLSTGELKVLLPLFKPYGRRQAFSGPVVTVKVFEDNVLVRALLESKD
Sbjct: 1   MVATSDICDANSSLLSTGELKVLLPLFKPYGRRQAFSGPVVTVKVFEDNVLVRALLESKD 60

Query: 61  HQGKVLVVDGGGSIRCALLGGNLGQIAHDNGWAGIVVNGCVRDVDEINGCDIGVWALGTN 120
           HQGKVLVVDGGGSIRCALLGGNLGQIAHDNGWAGIVVNGCVRDVDEINGCDIGVWALGTN
Sbjct: 61  HQGKVLVVDGGGSIRCALLGGNLGQIAHDNGWAGIVVNGCVRDVDEINGCDIGVWALGTN 120

Query: 121 PVKSKKKGLGDKHVSVNFAGSLINDGDWLWADNDGILISKHQLSATS 168
           PVKSKKKGLGDKHVSVNFAGSLINDGDWLWADNDGILISKHQLSATS
Sbjct: 121 PVKSKKKGLGDKHVSVNFAGSLINDGDWLWADNDGILISKHQLSATS 167

[Close]

BLAST of Spo19759.1 vs. NCBI nr
Match: gi|731339949|ref|XP_010681130.1| (PREDICTED: putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 3 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 307.4 bits (786), Expect = 1.600e-80
Identity = 146/167 (87.43%), Postives = 158/167 (94.61%), Query Frame = 1


		  
Query: 1   MVATSDICDANSSLLSTGELKVLLPLFKPYGRRQAFSGPVVTVKVFEDNVLVRALLESKD 60
           MVAT+DICDANSSLL+TGELKVLLPLF+ YGRRQAF+GPVVTVKVFEDNVLVRA+LESK 
Sbjct: 1   MVATTDICDANSSLLTTGELKVLLPLFRCYGRRQAFNGPVVTVKVFEDNVLVRAMLESKA 60

Query: 61  HQGKVLVVDGGGSIRCALLGGNLGQIAHDNGWAGIVVNGCVRDVDEINGCDIGVWALGTN 120
           H+GKVLVVDGGGS+RCALLGGNLGQIA DNGWAG+VV GC+RDVDEIN CDIGVWALG +
Sbjct: 61  HEGKVLVVDGGGSMRCALLGGNLGQIAQDNGWAGVVVYGCIRDVDEINSCDIGVWALGPS 120

Query: 121 PVKSKKKGLGDKHVSVNFAGSLINDGDWLWADNDGILISKHQLSATS 168
           PVKS KKGLGDKHVSV+FAG+LI DGDWLWADNDGILISKHQLSA S
Sbjct: 121 PVKSNKKGLGDKHVSVHFAGALIRDGDWLWADNDGILISKHQLSAPS 167

[Close]

BLAST of Spo19759.1 vs. NCBI nr
Match: gi|703074081|ref|XP_010089726.1| (hypothetical protein L484_013920 [Morus notabilis])

HSP 1 Score: 243.8 bits (621), Expect = 2.100e-61
Identity = 114/163 (69.94%), Postives = 144/163 (88.34%), Query Frame = 1


		  
Query: 2   VATSDICDANSSLLSTGELKVLLPLFKPYGRRQAFSGPVVTVKVFEDNVLVRALLESKDH 61
           +AT+++CD N++LL+TGEL VL PLFK YG+ +AF+GPVVT+KVFEDNVLVR +LE K  
Sbjct: 4   LATAEVCDTNAALLATGELHVLQPLFKTYGQCRAFAGPVVTLKVFEDNVLVREVLEGKG- 63

Query: 62  QGKVLVVDGGGSIRCALLGGNLGQIAHDNGWAGIVVNGCVRDVDEINGCDIGVWALGTNP 121
           +G+VLV+DGGGS+RCAL+GGNLGQ+A + GWAGIVVNGC+RDVDEINGCDIGV AL ++P
Sbjct: 64  EGRVLVIDGGGSMRCALVGGNLGQLAQNMGWAGIVVNGCIRDVDEINGCDIGVRALASHP 123

Query: 122 VKSKKKGLGDKHVSVNFAGSLINDGDWLWADNDGILISKHQLS 165
           VKS KKG+G+KHV V+  G+LI DG+WL+AD+DGILISK +LS
Sbjct: 124 VKSNKKGVGEKHVPVHVGGALIRDGEWLYADSDGILISKTELS 165

[Close]

BLAST of Spo19759.1 vs. NCBI nr
Match: gi|823170549|ref|XP_012484511.1| (PREDICTED: putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 3 isoform X2 [Gossypium raimondii])

HSP 1 Score: 243.0 bits (619), Expect = 3.600e-61
Identity = 114/163 (69.94%), Postives = 141/163 (86.50%), Query Frame = 1


		  
Query: 2   VATSDICDANSSLLSTGELKVLLPLFKPYGRRQAFSGPVVTVKVFEDNVLVRALLESKDH 61
           VAT+D+CD+N++LL  G+L+ L P+FK YGRR AFSGP+VTVKVFEDNVLVR LLE++ +
Sbjct: 59  VATADVCDSNAALLVNGDLRALDPIFKIYGRRLAFSGPIVTVKVFEDNVLVRQLLETEGN 118

Query: 62  QGKVLVVDGGGSIRCALLGGNLGQIAHDNGWAGIVVNGCVRDVDEINGCDIGVWALGTNP 121
            G+VLV+DGGGS RCAL+GGNL Q AHD  WAGI++NGC+RDVDEIN CDIGV ALG+NP
Sbjct: 119 -GRVLVIDGGGSTRCALVGGNLAQWAHDMAWAGIIMNGCIRDVDEINACDIGVRALGSNP 178

Query: 122 VKSKKKGLGDKHVSVNFAGSLINDGDWLWADNDGILISKHQLS 165
           +KS KK +GDKHV V  AG+LI+DG+WL+AD+DGILISK +LS
Sbjct: 179 LKSNKKAVGDKHVPVQIAGTLIHDGEWLYADSDGILISKTELS 220

[Close]

BLAST of Spo19759.1 vs. NCBI nr
Match: gi|823170546|ref|XP_012484510.1| (PREDICTED: putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 3 isoform X1 [Gossypium raimondii])

HSP 1 Score: 243.0 bits (619), Expect = 3.600e-61
Identity = 114/163 (69.94%), Postives = 141/163 (86.50%), Query Frame = 1


		  
Query: 2   VATSDICDANSSLLSTGELKVLLPLFKPYGRRQAFSGPVVTVKVFEDNVLVRALLESKDH 61
           VAT+D+CD+N++LL  G+L+ L P+FK YGRR AFSGP+VTVKVFEDNVLVR LLE++ +
Sbjct: 63  VATADVCDSNAALLVNGDLRALDPIFKIYGRRLAFSGPIVTVKVFEDNVLVRQLLETEGN 122

Query: 62  QGKVLVVDGGGSIRCALLGGNLGQIAHDNGWAGIVVNGCVRDVDEINGCDIGVWALGTNP 121
            G+VLV+DGGGS RCAL+GGNL Q AHD  WAGI++NGC+RDVDEIN CDIGV ALG+NP
Sbjct: 123 -GRVLVIDGGGSTRCALVGGNLAQWAHDMAWAGIIMNGCIRDVDEINACDIGVRALGSNP 182

Query: 122 VKSKKKGLGDKHVSVNFAGSLINDGDWLWADNDGILISKHQLS 165
           +KS KK +GDKHV V  AG+LI+DG+WL+AD+DGILISK +LS
Sbjct: 183 LKSNKKAVGDKHVPVQIAGTLIHDGEWLYADSDGILISKTELS 224

[Close]

BLAST of Spo19759.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QNY0_SPIOL (4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Spinacia oleracea GN=SOVF_157860 PE=3 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 4.700e-92
Identity = 167/167 (100.00%), Postives = 167/167 (100.00%), Query Frame = 1


		  
Query: 1   MVATSDICDANSSLLSTGELKVLLPLFKPYGRRQAFSGPVVTVKVFEDNVLVRALLESKD 60
           MVATSDICDANSSLLSTGELKVLLPLFKPYGRRQAFSGPVVTVKVFEDNVLVRALLESKD
Sbjct: 1   MVATSDICDANSSLLSTGELKVLLPLFKPYGRRQAFSGPVVTVKVFEDNVLVRALLESKD 60

Query: 61  HQGKVLVVDGGGSIRCALLGGNLGQIAHDNGWAGIVVNGCVRDVDEINGCDIGVWALGTN 120
           HQGKVLVVDGGGSIRCALLGGNLGQIAHDNGWAGIVVNGCVRDVDEINGCDIGVWALGTN
Sbjct: 61  HQGKVLVVDGGGSIRCALLGGNLGQIAHDNGWAGIVVNGCVRDVDEINGCDIGVWALGTN 120

Query: 121 PVKSKKKGLGDKHVSVNFAGSLINDGDWLWADNDGILISKHQLSATS 168
           PVKSKKKGLGDKHVSVNFAGSLINDGDWLWADNDGILISKHQLSATS
Sbjct: 121 PVKSKKKGLGDKHVSVNFAGSLINDGDWLWADNDGILISKHQLSATS 167

[Close]

BLAST of Spo19759.1 vs. UniProtKB/TrEMBL
Match: W9QJ00_9ROSA (4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Morus notabilis GN=L484_013920 PE=3 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 1.500e-61
Identity = 114/163 (69.94%), Postives = 144/163 (88.34%), Query Frame = 1


		  
Query: 2   VATSDICDANSSLLSTGELKVLLPLFKPYGRRQAFSGPVVTVKVFEDNVLVRALLESKDH 61
           +AT+++CD N++LL+TGEL VL PLFK YG+ +AF+GPVVT+KVFEDNVLVR +LE K  
Sbjct: 4   LATAEVCDTNAALLATGELHVLQPLFKTYGQCRAFAGPVVTLKVFEDNVLVREVLEGKG- 63

Query: 62  QGKVLVVDGGGSIRCALLGGNLGQIAHDNGWAGIVVNGCVRDVDEINGCDIGVWALGTNP 121
           +G+VLV+DGGGS+RCAL+GGNLGQ+A + GWAGIVVNGC+RDVDEINGCDIGV AL ++P
Sbjct: 64  EGRVLVIDGGGSMRCALVGGNLGQLAQNMGWAGIVVNGCIRDVDEINGCDIGVRALASHP 123

Query: 122 VKSKKKGLGDKHVSVNFAGSLINDGDWLWADNDGILISKHQLS 165
           VKS KKG+G+KHV V+  G+LI DG+WL+AD+DGILISK +LS
Sbjct: 124 VKSNKKGVGEKHVPVHVGGALIRDGEWLYADSDGILISKTELS 165

[Close]

BLAST of Spo19759.1 vs. UniProtKB/TrEMBL
Match: A0A0D2STL3_GOSRA (4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Gossypium raimondii GN=B456_006G075500 PE=3 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 2.500e-61
Identity = 114/163 (69.94%), Postives = 141/163 (86.50%), Query Frame = 1


		  
Query: 2   VATSDICDANSSLLSTGELKVLLPLFKPYGRRQAFSGPVVTVKVFEDNVLVRALLESKDH 61
           VAT+D+CD+N++LL  G+L+ L P+FK YGRR AFSGP+VTVKVFEDNVLVR LLE++ +
Sbjct: 63  VATADVCDSNAALLVNGDLRALDPIFKIYGRRLAFSGPIVTVKVFEDNVLVRQLLETEGN 122

Query: 62  QGKVLVVDGGGSIRCALLGGNLGQIAHDNGWAGIVVNGCVRDVDEINGCDIGVWALGTNP 121
            G+VLV+DGGGS RCAL+GGNL Q AHD  WAGI++NGC+RDVDEIN CDIGV ALG+NP
Sbjct: 123 -GRVLVIDGGGSTRCALVGGNLAQWAHDMAWAGIIMNGCIRDVDEINACDIGVRALGSNP 182

Query: 122 VKSKKKGLGDKHVSVNFAGSLINDGDWLWADNDGILISKHQLS 165
           +KS KK +GDKHV V  AG+LI+DG+WL+AD+DGILISK +LS
Sbjct: 183 LKSNKKAVGDKHVPVQIAGTLIHDGEWLYADSDGILISKTELS 224

[Close]

BLAST of Spo19759.1 vs. UniProtKB/TrEMBL
Match: A0A0D2RRV0_GOSRA (4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Gossypium raimondii GN=B456_006G075500 PE=3 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 2.500e-61
Identity = 114/163 (69.94%), Postives = 141/163 (86.50%), Query Frame = 1


		  
Query: 2   VATSDICDANSSLLSTGELKVLLPLFKPYGRRQAFSGPVVTVKVFEDNVLVRALLESKDH 61
           VAT+D+CD+N++LL  G+L+ L P+FK YGRR AFSGP+VTVKVFEDNVLVR LLE++ +
Sbjct: 59  VATADVCDSNAALLVNGDLRALDPIFKIYGRRLAFSGPIVTVKVFEDNVLVRQLLETEGN 118

Query: 62  QGKVLVVDGGGSIRCALLGGNLGQIAHDNGWAGIVVNGCVRDVDEINGCDIGVWALGTNP 121
            G+VLV+DGGGS RCAL+GGNL Q AHD  WAGI++NGC+RDVDEIN CDIGV ALG+NP
Sbjct: 119 -GRVLVIDGGGSTRCALVGGNLAQWAHDMAWAGIIMNGCIRDVDEINACDIGVRALGSNP 178

Query: 122 VKSKKKGLGDKHVSVNFAGSLINDGDWLWADNDGILISKHQLS 165
           +KS KK +GDKHV V  AG+LI+DG+WL+AD+DGILISK +LS
Sbjct: 179 LKSNKKAVGDKHVPVQIAGTLIHDGEWLYADSDGILISKTELS 220

[Close]

BLAST of Spo19759.1 vs. UniProtKB/TrEMBL
Match: B9ST87_RICCO (4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Ricinus communis GN=RCOM_0364170 PE=3 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 3.300e-61
Identity = 110/163 (67.48%), Postives = 147/163 (90.18%), Query Frame = 1


		  
Query: 2   VATSDICDANSSLLSTGELKVLLPLFKPYGRRQAFSGPVVTVKVFEDNVLVRALLESKDH 61
           VAT++ CD+N++L ++G+L+ L P+FK YG+R+AFSGP+VT+KVFEDNVLVR LLE++  
Sbjct: 4   VATAEACDSNAALSASGDLRALQPVFKIYGQRRAFSGPIVTLKVFEDNVLVRELLETRG- 63

Query: 62  QGKVLVVDGGGSIRCALLGGNLGQIAHDNGWAGIVVNGCVRDVDEINGCDIGVWALGTNP 121
           +G+VLV+DGGGS+RCAL+GGNLGQ+A + GWAGI+VNGC+RDVDEINGCDIGV AL ++P
Sbjct: 64  EGRVLVIDGGGSMRCALVGGNLGQLAQNMGWAGIIVNGCIRDVDEINGCDIGVRALASHP 123

Query: 122 VKSKKKGLGDKHVSVNFAGSLINDGDWLWADNDGILISKHQLS 165
           +KS KKG+G+KHV+V+ AG+LI DG+WL+AD+DGILISK +LS
Sbjct: 124 MKSNKKGMGEKHVTVHIAGTLIRDGEWLYADSDGILISKSELS 165

[Close]

BLAST of Spo19759.1 vs. ExPASy Swiss-Prot
Match: RRAA3_ARATH (Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 3 OS=Arabidopsis thaliana GN=At5g56260 PE=1 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 8.000e-61
Identity = 109/162 (67.28%), Postives = 140/162 (86.42%), Query Frame = 1


		  
Query: 3   ATSDICDANSSLLSTGELKVLLPLFKPYGRRQAFSGPVVTVKVFEDNVLVRALLESKDHQ 62
           AT++ CD+N+ L+S G+L+ L P+FK YG+R+ FSGP+VT+KVFEDNVLVR  LE+K  +
Sbjct: 5   ATAEACDSNAELISNGDLRALHPIFKIYGQRRCFSGPIVTLKVFEDNVLVRNQLETKG-E 64

Query: 63  GKVLVVDGGGSIRCALLGGNLGQIAHDNGWAGIVVNGCVRDVDEINGCDIGVWALGTNPV 122
           G VLV+DGGGS+RCAL+GGNLGQ+A +NGW+GIVVNGCVRDVDEIN CD+GV ALG+NP+
Sbjct: 65  GGVLVIDGGGSMRCALVGGNLGQLAQNNGWSGIVVNGCVRDVDEINDCDVGVRALGSNPL 124

Query: 123 KSKKKGLGDKHVSVNFAGSLINDGDWLWADNDGILISKHQLS 165
           KS KKG G+K+V V+  G+LI DG+WL+AD+DGILISK +LS
Sbjct: 125 KSTKKGHGEKNVPVHIGGTLIRDGEWLYADSDGILISKTELS 165

[Close]

BLAST of Spo19759.1 vs. ExPASy Swiss-Prot
Match: RRAA1_ARATH (Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 1 OS=Arabidopsis thaliana GN=At3g02770 PE=2 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 1.100e-57
Identity = 104/163 (63.80%), Postives = 135/163 (82.82%), Query Frame = 1


		  
Query: 2   VATSDICDANSSLLSTGELKVLLPLFKPYGRRQAFSGPVVTVKVFEDNVLVRALLESKDH 61
           V T+++CDAN  ++ +G+L+ L P+F+ YGRRQ FSGPVVTVKVFEDN L+R  LE K +
Sbjct: 4   VTTAEVCDANQEMIRSGQLRALQPVFQIYGRRQIFSGPVVTVKVFEDNGLIRHFLEEKGN 63

Query: 62  QGKVLVVDGGGSIRCALLGGNLGQIAHDNGWAGIVVNGCVRDVDEINGCDIGVWALGTNP 121
            G+VLVVDGGGS+RCA+LGGN    A +NGWAGI+VNGC+RDVDEINGCDIGV AL ++P
Sbjct: 64  -GRVLVVDGGGSLRCAILGGNPVVQAQNNGWAGIIVNGCIRDVDEINGCDIGVRALASHP 123

Query: 122 VKSKKKGLGDKHVSVNFAGSLINDGDWLWADNDGILISKHQLS 165
           +K+ KKGLG++ VS+N AG+ I DG+WL+AD DGIL+S+ +LS
Sbjct: 124 IKASKKGLGEQRVSLNIAGTRICDGEWLYADTDGILVSQIELS 165

[Close]

BLAST of Spo19759.1 vs. ExPASy Swiss-Prot
Match: RRAA2_ARATH (Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 OS=Arabidopsis thaliana GN=At5g16450 PE=1 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 3.200e-57
Identity = 105/163 (64.42%), Postives = 133/163 (81.60%), Query Frame = 1


		  
Query: 2   VATSDICDANSSLLSTGELKVLLPLFKPYGRRQAFSGPVVTVKVFEDNVLVRALLESKDH 61
           V T+++CDAN  L+ +G+L+ L P+F+ YGRRQ FSGPVVTVKVFEDN L+R  +E K +
Sbjct: 4   VTTAEVCDANQELIRSGQLRALQPIFQIYGRRQIFSGPVVTVKVFEDNGLIRQFIEEKGN 63

Query: 62  QGKVLVVDGGGSIRCALLGGNLGQIAHDNGWAGIVVNGCVRDVDEINGCDIGVWALGTNP 121
            G+VLVVDGGGS RCA+LGGN    A +NGWAGIVVNGC+RDVDEINGCDIGV AL ++P
Sbjct: 64  -GRVLVVDGGGSQRCAILGGNPVVQAQNNGWAGIVVNGCIRDVDEINGCDIGVRALASHP 123

Query: 122 VKSKKKGLGDKHVSVNFAGSLINDGDWLWADNDGILISKHQLS 165
           +K+ KKGLG++ V VN AG+ I DG+WL+AD DGIL+S+ +LS
Sbjct: 124 IKASKKGLGEQRVPVNIAGTRICDGEWLYADTDGILVSQIELS 165

[Close]

BLAST of Spo19759.1 vs. ExPASy Swiss-Prot
Match: RRAAH_CUPPJ (Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) GN=Reut_A1963 PE=3 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 1.700e-50
Identity = 88/162 (54.32%), Postives = 125/162 (77.16%), Query Frame = 1


		  
Query: 4   TSDICDANSSLLSTGELKVLLPLFKPYGRRQAFSGPVVTVKVFEDNVLVRALLESKDHQG 63
           T+D+CDAN   L+ G L+V+ P+F+ +G++QAF+GP  T+K+FEDN LVR  LES   QG
Sbjct: 5   TTDLCDANEPRLADGTLRVMAPVFRAFGKQQAFAGPAATLKIFEDNSLVREALESPG-QG 64

Query: 64  KVLVVDGGGSIRCALLGGNLGQIAHDNGWAGIVVNGCVRDVDEINGCDIGVWALGTNPVK 123
           +VLVVDGGGS+RCAL+GGNLG +A  NGW GI+VNGCVRD  E++ CDIG+ AL  +P K
Sbjct: 65  RVLVVDGGGSLRCALVGGNLGLLAEKNGWVGIIVNGCVRDTGELDVCDIGIRALAAHPQK 124

Query: 124 SKKKGLGDKHVSVNFAGSLINDGDWLWADNDGILISKHQLSA 166
           S+K+G G++ V+V   G+++  G+W++ D DG+L+S+ +L A
Sbjct: 125 SQKRGAGERDVAVQMPGAVVRPGNWIYVDADGVLVSEEKLGA 165

[Close]

BLAST of Spo19759.1 vs. ExPASy Swiss-Prot
Match: RRAAH_RALSO (Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Ralstonia solanacearum (strain GMI1000) GN=RSc1354 PE=3 SV=2)

HSP 1 Score: 192.2 bits (487), Expect = 4.600e-48
Identity = 84/163 (51.53%), Postives = 122/163 (74.85%), Query Frame = 1


		  
Query: 1   MVATSDICDANSSLLSTGELKVLLPLFKPYGRRQAFSGPVVTVKVFEDNVLVRALLESKD 60
           M+  +D+CDA+   L++G ++VL P+F+ +G R AF+GP  T+KVFEDN LVRA+LE   
Sbjct: 2   MIPVTDLCDAHEDQLASGAVRVLAPVFRSFGARAAFAGPAATLKVFEDNGLVRAMLEQPG 61

Query: 61  HQGKVLVVDGGGSIRCALLGGNLGQIAHDNGWAGIVVNGCVRDVDEINGCDIGVWALGTN 120
            +G+VLVVDGGGS+RCAL+GGNL ++A DNGW G++VNGCVRD  E+  C++GV AL  +
Sbjct: 62  -EGRVLVVDGGGSLRCALVGGNLAKLAEDNGWEGVLVNGCVRDTRELAACNVGVHALAAH 121

Query: 121 PVKSKKKGLGDKHVSVNFAGSLINDGDWLWADNDGILISKHQL 164
           P KS KK  G++ + V   G+ I+ G+W++ D DG+L+S+ +L
Sbjct: 122 PRKSFKKNQGERDIGVQMPGAYIHPGEWIYGDEDGVLVSRERL 163

[Close]

BLAST of Spo19759.1 vs. TAIR (Arabidopsis)
Match: AT5G56260.1 (Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase)

HSP 1 Score: 234.6 bits (597), Expect = 4.500e-62
Identity = 109/162 (67.28%), Postives = 140/162 (86.42%), Query Frame = 1


		  
Query: 3   ATSDICDANSSLLSTGELKVLLPLFKPYGRRQAFSGPVVTVKVFEDNVLVRALLESKDHQ 62
           AT++ CD+N+ L+S G+L+ L P+FK YG+R+ FSGP+VT+KVFEDNVLVR  LE+K  +
Sbjct: 5   ATAEACDSNAELISNGDLRALHPIFKIYGQRRCFSGPIVTLKVFEDNVLVRNQLETKG-E 64

Query: 63  GKVLVVDGGGSIRCALLGGNLGQIAHDNGWAGIVVNGCVRDVDEINGCDIGVWALGTNPV 122
           G VLV+DGGGS+RCAL+GGNLGQ+A +NGW+GIVVNGCVRDVDEIN CD+GV ALG+NP+
Sbjct: 65  GGVLVIDGGGSMRCALVGGNLGQLAQNNGWSGIVVNGCVRDVDEINDCDVGVRALGSNPL 124

Query: 123 KSKKKGLGDKHVSVNFAGSLINDGDWLWADNDGILISKHQLS 165
           KS KKG G+K+V V+  G+LI DG+WL+AD+DGILISK +LS
Sbjct: 125 KSTKKGHGEKNVPVHIGGTLIRDGEWLYADSDGILISKTELS 165

[Close]

BLAST of Spo19759.1 vs. TAIR (Arabidopsis)
Match: AT3G02770.1 (Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase)

HSP 1 Score: 224.2 bits (570), Expect = 6.100e-59
Identity = 104/163 (63.80%), Postives = 135/163 (82.82%), Query Frame = 1


		  
Query: 2   VATSDICDANSSLLSTGELKVLLPLFKPYGRRQAFSGPVVTVKVFEDNVLVRALLESKDH 61
           V T+++CDAN  ++ +G+L+ L P+F+ YGRRQ FSGPVVTVKVFEDN L+R  LE K +
Sbjct: 4   VTTAEVCDANQEMIRSGQLRALQPVFQIYGRRQIFSGPVVTVKVFEDNGLIRHFLEEKGN 63

Query: 62  QGKVLVVDGGGSIRCALLGGNLGQIAHDNGWAGIVVNGCVRDVDEINGCDIGVWALGTNP 121
            G+VLVVDGGGS+RCA+LGGN    A +NGWAGI+VNGC+RDVDEINGCDIGV AL ++P
Sbjct: 64  -GRVLVVDGGGSLRCAILGGNPVVQAQNNGWAGIIVNGCIRDVDEINGCDIGVRALASHP 123

Query: 122 VKSKKKGLGDKHVSVNFAGSLINDGDWLWADNDGILISKHQLS 165
           +K+ KKGLG++ VS+N AG+ I DG+WL+AD DGIL+S+ +LS
Sbjct: 124 IKASKKGLGEQRVSLNIAGTRICDGEWLYADTDGILVSQIELS 165

[Close]

BLAST of Spo19759.1 vs. TAIR (Arabidopsis)
Match: AT5G16450.1 (Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase)

HSP 1 Score: 222.6 bits (566), Expect = 1.800e-58
Identity = 105/163 (64.42%), Postives = 133/163 (81.60%), Query Frame = 1


		  
Query: 2   VATSDICDANSSLLSTGELKVLLPLFKPYGRRQAFSGPVVTVKVFEDNVLVRALLESKDH 61
           V T+++CDAN  L+ +G+L+ L P+F+ YGRRQ FSGPVVTVKVFEDN L+R  +E K +
Sbjct: 4   VTTAEVCDANQELIRSGQLRALQPIFQIYGRRQIFSGPVVTVKVFEDNGLIRQFIEEKGN 63

Query: 62  QGKVLVVDGGGSIRCALLGGNLGQIAHDNGWAGIVVNGCVRDVDEINGCDIGVWALGTNP 121
            G+VLVVDGGGS RCA+LGGN    A +NGWAGIVVNGC+RDVDEINGCDIGV AL ++P
Sbjct: 64  -GRVLVVDGGGSQRCAILGGNPVVQAQNNGWAGIVVNGCIRDVDEINGCDIGVRALASHP 123

Query: 122 VKSKKKGLGDKHVSVNFAGSLINDGDWLWADNDGILISKHQLS 165
           +K+ KKGLG++ V VN AG+ I DG+WL+AD DGIL+S+ +LS
Sbjct: 124 IKASKKGLGEQRVPVNIAGTRICDGEWLYADTDGILVSQIELS 165

The following BLAST results are available for this feature:

BLAST of Spo19759.1 vs. NCBI nr
BLAST of Spo19759.1 vs. UniProtKB/TrEMBL
BLAST of Spo19759.1 vs. ExPASy Swiss-Prot
BLAST of Spo19759.1 vs. TAIR (Arabidopsis)

BLAST of Spo19759.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902177580\|gb\|KNA08974.1\|	6.7e-92	100.	hypothetical protein SOVF_1578...	[more]
gi\|731339949\|ref\|XP_010681130.1\|	1.6e-80	87.4	PREDICTED: putative 4-hydroxy-...	[more]
gi\|703074081\|ref\|XP_010089726.1\|	2.1e-61	69.9	hypothetical protein L484_0139...	[more]
gi\|823170549\|ref\|XP_012484511.1\|	3.6e-61	69.9	PREDICTED: putative 4-hydroxy-...	[more]
gi\|823170546\|ref\|XP_012484510.1\|	3.6e-61	69.9	PREDICTED: putative 4-hydroxy-...	[more]

BLAST of Spo19759.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9QNY0_SPIOL	4.7e-92	100.	4-hydroxy-4-methyl-2-oxoglutar...	[more]
W9QJ00_9ROSA	1.5e-61	69.9	4-hydroxy-4-methyl-2-oxoglutar...	[more]
A0A0D2STL3_GOSRA	2.5e-61	69.9	4-hydroxy-4-methyl-2-oxoglutar...	[more]
A0A0D2RRV0_GOSRA	2.5e-61	69.9	4-hydroxy-4-methyl-2-oxoglutar...	[more]
B9ST87_RICCO	3.3e-61	67.4	4-hydroxy-4-methyl-2-oxoglutar...	[more]

BLAST of Spo19759.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
RRAA3_ARATH	8.0e-61	67.2	Putative 4-hydroxy-4-methyl-2-...	[more]
RRAA1_ARATH	1.1e-57	63.8	Putative 4-hydroxy-4-methyl-2-...	[more]
RRAA2_ARATH	3.2e-57	64.4	Putative 4-hydroxy-4-methyl-2-...	[more]
RRAAH_CUPPJ	1.7e-50	54.3	Putative 4-hydroxy-4-methyl-2-...	[more]
RRAAH_RALSO	4.6e-48	51.5	Putative 4-hydroxy-4-methyl-2-...	[more]

BLAST of Spo19759.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 3


Match Name	E-value	Identity	Description
AT5G56260.1	4.5e-62	67.2	Ribonuclease E inhibitor RraA/...	[more]
AT3G02770.1	6.1e-59	63.8	Ribonuclease E inhibitor RraA/...	[more]
AT5G16450.1	1.8e-58	64.4	Ribonuclease E inhibitor RraA/...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005493	Ribonuclease E inhibitor RraA/RraA-like protein	GENE3D	3.50.30.40		coord: 2..163 score: 5.9
IPR005493	Ribonuclease E inhibitor RraA/RraA-like protein	PFAM	PF03737	Methyltransf_6	coord: 4..157 score: 1.8
IPR005493	Ribonuclease E inhibitor RraA/RraA-like protein	unknown	SSF89562	RraA-like	coord: 2..163 score: 1.19
IPR010203	Regulator of ribonuclease activity A	TIGRFAMs	TIGR01935	TIGR01935	coord: 4..159 score: 1.6
None	No IPR available	PANTHER	PTHR33254	FAMILY NOT NAMED	coord: 1..163 score: 6.1
None	No IPR available	PANTHER	PTHR33254:SF4	REGULATOR OF RIBONUCLEASE-LIKE PROTEIN 1-RELATED	coord: 1..163 score: 6.1

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006525	arginine metabolic process
biological_process	GO:0043069	negative regulation of programmed cell death
biological_process	GO:0032259	methylation
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0006560	proline metabolic process
biological_process	GO:0006090	pyruvate metabolic process
biological_process	GO:0051252	regulation of RNA metabolic process
biological_process	GO:0006457	protein folding
biological_process	GO:0018874	benzoate metabolic process
biological_process	GO:0018279	protein N-linked glycosylation via asparagine
cellular_component	GO:0008250	oligosaccharyltransferase complex
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0008428	ribonuclease inhibitor activity
molecular_function	GO:0008948	oxaloacetate decarboxylase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004579	dolichyl-diphosphooligosaccharide-protein glycotransferase activity
molecular_function	GO:0047443	4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
molecular_function	GO:0008168	methyltransferase activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo25659	0.72	Barchart \| Table
Spo11446	0.69	Barchart \| Table
Spo23384	0.67	Barchart \| Table
Spo16797	0.65	Barchart \| Table
Spo22811	0.65	Barchart \| Table