Spo20170 (gene)

Overview
NameSpo20170
Typegene
OrganismSpinacia oleracea (Spinach)
DescriptionCysteine proteinase inhibitor (Cystatin)
LocationSuper_scaffold_125 : 473968 .. 474318 (+)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGAGCCGCCACTTTCTCCTCCTTGCCCTCTCTCTCCTCGCTGTAGTCTCCGCCGTATCCGCGTCCAGGAAACACGGCGGCGCACTCGTCGGAGGATACTCGCCGATCAAGGACGTCTCCGATCCGTACGTTCAAGGCGTCGGGCAATTCGCGGTTACCGAATACAACAAAGATCAAAAGGCTCACCTGAAATTTGTGAAGGTGGTGAAAGGTGAGTCACAGGTTGTCTCCGGGACGAATTACCGTCTTTTTATCGAGGCCGATGATAATAGCCAGTACGTCGCTATTGTCTATGATGTGCCGTGGCGACATCAGAGGAGTCTAACCTCATTTACACCTGCTGCTTAG

mRNA sequence

ATGTCGAGCCGCCACTTTCTCCTCCTTGCCCTCTCTCTCCTCGCTGTAGTCTCCGCCGTATCCGCGTCCAGGAAACACGGCGGCGCACTCGTCGGAGGATACTCGCCGATCAAGGACGTCTCCGATCCGTACGTTCAAGGCGTCGGGCAATTCGCGGTTACCGAATACAACAAAGATCAAAAGGCTCACCTGAAATTTGTGAAGGTGGTGAAAGGTGAGTCACAGGTTGTCTCCGGGACGAATTACCGTCTTTTTATCGAGGCCGATGATAATAGCCAGTACGTCGCTATTGTCTATGATGTGCCGTGGCGACATCAGAGGAGTCTAACCTCATTTACACCTGCTGCTTAG

Coding sequence (CDS)

ATGTCGAGCCGCCACTTTCTCCTCCTTGCCCTCTCTCTCCTCGCTGTAGTCTCCGCCGTATCCGCGTCCAGGAAACACGGCGGCGCACTCGTCGGAGGATACTCGCCGATCAAGGACGTCTCCGATCCGTACGTTCAAGGCGTCGGGCAATTCGCGGTTACCGAATACAACAAAGATCAAAAGGCTCACCTGAAATTTGTGAAGGTGGTGAAAGGTGAGTCACAGGTTGTCTCCGGGACGAATTACCGTCTTTTTATCGAGGCCGATGATAATAGCCAGTACGTCGCTATTGTCTATGATGTGCCGTGGCGACATCAGAGGAGTCTAACCTCATTTACACCTGCTGCTTAG

Protein sequence

MSSRHFLLLALSLLAVVSAVSASRKHGGALVGGYSPIKDVSDPYVQGVGQFAVTEYNKDQKAHLKFVKVVKGESQVVSGTNYRLFIEADDNSQYVAIVYDVPWRHQRSLTSFTPAA
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo20170.1Spo20170.1mRNA


Homology
[Close]
BLAST of Spo20170.1 vs. NCBI nr
Match: gi|902233059|gb|KNA22917.1| (hypothetical protein SOVF_029630 [Spinacia oleracea])

HSP 1 Score: 228.4 bits (581), Expect = 6.400e-57
Identity = 116/116 (100.00%), Postives = 116/116 (100.00%), Query Frame = 1 

		  

Query: 1   MSSRHFLLLALSLLAVVSAVSASRKHGGALVGGYSPIKDVSDPYVQGVGQFAVTEYNKDQ 60
           MSSRHFLLLALSLLAVVSAVSASRKHGGALVGGYSPIKDVSDPYVQGVGQFAVTEYNKDQ
Sbjct: 1   MSSRHFLLLALSLLAVVSAVSASRKHGGALVGGYSPIKDVSDPYVQGVGQFAVTEYNKDQ 60

Query: 61  KAHLKFVKVVKGESQVVSGTNYRLFIEADDNSQYVAIVYDVPWRHQRSLTSFTPAA 117
           KAHLKFVKVVKGESQVVSGTNYRLFIEADDNSQYVAIVYDVPWRHQRSLTSFTPAA
Sbjct: 61  KAHLKFVKVVKGESQVVSGTNYRLFIEADDNSQYVAIVYDVPWRHQRSLTSFTPAA 116
[Close]
BLAST of Spo20170.1 vs. NCBI nr
Match: gi|731350829|ref|XP_010686713.1| (PREDICTED: cysteine proteinase inhibitor 1 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 149.1 bits (375), Expect = 4.900e-33
Identity = 74/112 (66.07%), Postives = 92/112 (82.14%), Query Frame = 1 

		  

Query: 1   MSSRHFLLLALSLLAVVSAVSASRKHGGALVGGYSPIKDVSDPYVQGVGQFAVTEYNKDQ 60
           MS+RH LL+  SLLAV+SA SA+ + G  L GGY PIKD+ DPYVQ + +FAV E+NK++
Sbjct: 1   MSTRHILLI-FSLLAVISAASAATRRGPVL-GGYRPIKDIKDPYVQEIAKFAVDEHNKEE 60

Query: 61  KAHLKFVKVVKGESQVVSGTNYRLFIEADDNSQYVAIVYDVPWRHQRSLTSF 113
            +HLK VK+VKGESQVVSGTNYRL + ADD+ +YVA+VYD PW+HQRSLTSF
Sbjct: 61  GSHLKLVKIVKGESQVVSGTNYRLTLSADDSQKYVAVVYDKPWQHQRSLTSF 110
[Close]
BLAST of Spo20170.1 vs. NCBI nr
Match: gi|703092669|ref|XP_010094696.1| (Cysteine proteinase inhibitor 5 [Morus notabilis])

HSP 1 Score: 117.1 bits (292), Expect = 2.100e-23
Identity = 61/115 (53.04%), Postives = 82/115 (71.30%), Query Frame = 1 

		  

Query: 5   HFLLLALSLLAVVSAVSASRKHGGALVGGYSPIKDVSDPYVQGVGQFAVTEYNKDQKAHL 64
           HFLL+ LS+L V  A     K  G LVGG++PIKD+SDP+V+ +  FAV+EYNK  KA L
Sbjct: 4   HFLLVILSVLVVPLAA----KRTGPLVGGWTPIKDLSDPHVKEIANFAVSEYNKQSKASL 63

Query: 65  KFVKVVKGESQVVSGTNYRLFIEADDNS---QYVAIVYDVPWRHQRSLTSFTPAA 117
           K   +VKGE+QVV+GTNYRL +   + +   +Y A+V++ PW+H R+LTSF PAA
Sbjct: 64  KLKSIVKGETQVVAGTNYRLVVAVTNGAAAERYEAVVWEKPWQHFRNLTSFKPAA 114
[Close]
BLAST of Spo20170.1 vs. NCBI nr
Match: gi|746657137|gb|AJD79055.1| (CPI-4 [Morus alba var. atropurpurea])

HSP 1 Score: 116.7 bits (291), Expect = 2.700e-23
Identity = 61/115 (53.04%), Postives = 82/115 (71.30%), Query Frame = 1 

		  

Query: 5   HFLLLALSLLAVVSAVSASRKHGGALVGGYSPIKDVSDPYVQGVGQFAVTEYNKDQKAHL 64
           HFLL+ LS+L V  A     K  G LVGG++PIKD+SDP+V+ +  FAV+EYNK  KA L
Sbjct: 4   HFLLVILSVLVVPLAA----KRTGPLVGGWTPIKDLSDPHVKEIANFAVSEYNKQSKASL 63

Query: 65  KFVKVVKGESQVVSGTNYRLFIEADDNS---QYVAIVYDVPWRHQRSLTSFTPAA 117
           K   +VKGE+QVV+GTNYRL +   + +   +Y A+V++ PW+H R+LTSF PAA
Sbjct: 64  KLKSIVKGETQVVAGTNYRLVLAVKNGATAERYEAVVWEKPWQHFRNLTSFKPAA 114
[Close]
BLAST of Spo20170.1 vs. NCBI nr
Match: gi|388496176|gb|AFK36154.1| (unknown [Lotus japonicus])

HSP 1 Score: 112.5 bits (280), Expect = 5.100e-22
Identity = 58/114 (50.88%), Postives = 80/114 (70.18%), Query Frame = 1 

		  

Query: 7   LLLALSLLAVVSAVSASRKHGGALVGGYSPIKDVSDPYVQGVGQFAVTEYNKDQKAHLKF 66
           L+++L +L+ VS  SA+ +  GA VGGY+PIKD++DP+V+ +  FAV+EY+K   A L+ 
Sbjct: 7   LIVSLLILSAVSGFSAALRQKGAFVGGYTPIKDLNDPHVKEIANFAVSEYDKQSGAKLRL 66

Query: 67  VKVVKGESQVVSGTNYRLFIEADDNSQ------YVAIVYDVPWRHQRSLTSFTP 115
           VKVV G+SQVV+GTNYRL I A   S+      Y A+VY+  W H R+LTSF P
Sbjct: 67  VKVVSGDSQVVAGTNYRLVIAAKGGSRSAAASNYEALVYERSWEHFRNLTSFKP 120
[Close]
BLAST of Spo20170.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RVP8_SPIOL (Cysteine proteinase inhibitor OS=Spinacia oleracea GN=SOVF_029630 PE=3 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 4.400e-57
Identity = 116/116 (100.00%), Postives = 116/116 (100.00%), Query Frame = 1 

		  

Query: 1   MSSRHFLLLALSLLAVVSAVSASRKHGGALVGGYSPIKDVSDPYVQGVGQFAVTEYNKDQ 60
           MSSRHFLLLALSLLAVVSAVSASRKHGGALVGGYSPIKDVSDPYVQGVGQFAVTEYNKDQ
Sbjct: 1   MSSRHFLLLALSLLAVVSAVSASRKHGGALVGGYSPIKDVSDPYVQGVGQFAVTEYNKDQ 60

Query: 61  KAHLKFVKVVKGESQVVSGTNYRLFIEADDNSQYVAIVYDVPWRHQRSLTSFTPAA 117
           KAHLKFVKVVKGESQVVSGTNYRLFIEADDNSQYVAIVYDVPWRHQRSLTSFTPAA
Sbjct: 61  KAHLKFVKVVKGESQVVSGTNYRLFIEADDNSQYVAIVYDVPWRHQRSLTSFTPAA 116
[Close]
BLAST of Spo20170.1 vs. UniProtKB/TrEMBL
Match: A0A0J8EMH9_BETVU (Cysteine proteinase inhibitor OS=Beta vulgaris subsp. vulgaris GN=BVRB_8g184860 PE=3 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 3.400e-33
Identity = 74/112 (66.07%), Postives = 92/112 (82.14%), Query Frame = 1 

		  

Query: 1   MSSRHFLLLALSLLAVVSAVSASRKHGGALVGGYSPIKDVSDPYVQGVGQFAVTEYNKDQ 60
           MS+RH LL+  SLLAV+SA SA+ + G  L GGY PIKD+ DPYVQ + +FAV E+NK++
Sbjct: 1   MSTRHILLI-FSLLAVISAASAATRRGPVL-GGYRPIKDIKDPYVQEIAKFAVDEHNKEE 60

Query: 61  KAHLKFVKVVKGESQVVSGTNYRLFIEADDNSQYVAIVYDVPWRHQRSLTSF 113
            +HLK VK+VKGESQVVSGTNYRL + ADD+ +YVA+VYD PW+HQRSLTSF
Sbjct: 61  GSHLKLVKIVKGESQVVSGTNYRLTLSADDSQKYVAVVYDKPWQHQRSLTSF 110
[Close]
BLAST of Spo20170.1 vs. UniProtKB/TrEMBL
Match: W9R7F6_9ROSA (Cysteine proteinase inhibitor OS=Morus notabilis GN=L484_004273 PE=3 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 1.400e-23
Identity = 61/115 (53.04%), Postives = 82/115 (71.30%), Query Frame = 1 

		  

Query: 5   HFLLLALSLLAVVSAVSASRKHGGALVGGYSPIKDVSDPYVQGVGQFAVTEYNKDQKAHL 64
           HFLL+ LS+L V  A     K  G LVGG++PIKD+SDP+V+ +  FAV+EYNK  KA L
Sbjct: 4   HFLLVILSVLVVPLAA----KRTGPLVGGWTPIKDLSDPHVKEIANFAVSEYNKQSKASL 63

Query: 65  KFVKVVKGESQVVSGTNYRLFIEADDNS---QYVAIVYDVPWRHQRSLTSFTPAA 117
           K   +VKGE+QVV+GTNYRL +   + +   +Y A+V++ PW+H R+LTSF PAA
Sbjct: 64  KLKSIVKGETQVVAGTNYRLVVAVTNGAAAERYEAVVWEKPWQHFRNLTSFKPAA 114
[Close]
BLAST of Spo20170.1 vs. UniProtKB/TrEMBL
Match: A0A0B4ZWL9_MORAL (Cysteine proteinase inhibitor OS=Morus alba var. atropurpurea PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 1.900e-23
Identity = 61/115 (53.04%), Postives = 82/115 (71.30%), Query Frame = 1 

		  

Query: 5   HFLLLALSLLAVVSAVSASRKHGGALVGGYSPIKDVSDPYVQGVGQFAVTEYNKDQKAHL 64
           HFLL+ LS+L V  A     K  G LVGG++PIKD+SDP+V+ +  FAV+EYNK  KA L
Sbjct: 4   HFLLVILSVLVVPLAA----KRTGPLVGGWTPIKDLSDPHVKEIANFAVSEYNKQSKASL 63

Query: 65  KFVKVVKGESQVVSGTNYRLFIEADDNS---QYVAIVYDVPWRHQRSLTSFTPAA 117
           K   +VKGE+QVV+GTNYRL +   + +   +Y A+V++ PW+H R+LTSF PAA
Sbjct: 64  KLKSIVKGETQVVAGTNYRLVLAVKNGATAERYEAVVWEKPWQHFRNLTSFKPAA 114
[Close]
BLAST of Spo20170.1 vs. UniProtKB/TrEMBL
Match: I3S7B2_LOTJA (Cysteine proteinase inhibitor OS=Lotus japonicus PE=2 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 3.600e-22
Identity = 58/114 (50.88%), Postives = 80/114 (70.18%), Query Frame = 1 

		  

Query: 7   LLLALSLLAVVSAVSASRKHGGALVGGYSPIKDVSDPYVQGVGQFAVTEYNKDQKAHLKF 66
           L+++L +L+ VS  SA+ +  GA VGGY+PIKD++DP+V+ +  FAV+EY+K   A L+ 
Sbjct: 7   LIVSLLILSAVSGFSAALRQKGAFVGGYTPIKDLNDPHVKEIANFAVSEYDKQSGAKLRL 66

Query: 67  VKVVKGESQVVSGTNYRLFIEADDNSQ------YVAIVYDVPWRHQRSLTSFTP 115
           VKVV G+SQVV+GTNYRL I A   S+      Y A+VY+  W H R+LTSF P
Sbjct: 67  VKVVSGDSQVVAGTNYRLVIAAKGGSRSAAASNYEALVYERSWEHFRNLTSFKP 120
[Close]
BLAST of Spo20170.1 vs. ExPASy Swiss-Prot
Match: CYT5_ARATH (Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana GN=CYS5 PE=2 SV=2)

HSP 1 Score: 107.8 bits (268), Expect = 7.900e-23
Identity = 62/119 (52.10%), Postives = 82/119 (68.91%), Query Frame = 1 

		  

Query: 1   MSSRHFLLLALSLLAVVSAVSASRKHGGALVGGYSPIKDVSDPYVQGVGQFAVTEYNKDQ 60
           M+S+   LL LSL+ V+  + AS     A VGG+SPI +V+DP V  +G+FAV+EYNK  
Sbjct: 1   MTSKVVFLLLLSLVVVLLPLYAS---AAARVGGWSPISNVTDPQVVEIGEFAVSEYNKRS 60

Query: 61  KAHLKFVKVVKGESQVVSGTNYRLFIEADD----NSQYVAIVYDVPWRHQRSLTSFTPA 116
           ++ LKF  VV GE+QVVSGTNYRL + A+D    +  Y+AIV+D PW   R+LTSF PA
Sbjct: 61  ESGLKFETVVSGETQVVSGTNYRLKVAANDGDGVSKNYLAIVWDKPWMKFRNLTSFEPA 116
[Close]
BLAST of Spo20170.1 vs. ExPASy Swiss-Prot
Match: CYT8_ORYSJ (Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica GN=Os03g0429000 PE=2 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 2.400e-19
Identity = 55/116 (47.41%), Postives = 72/116 (62.07%), Query Frame = 1 

		  

Query: 9   LALSLLAVVSAVSASRKHG----GALVGGYSPIKDVSDPYVQGVGQFAVTEYNKDQKAHL 68
           L L+LL  VSA +A++  G    G LVGG+SPI DV DP++Q +G +AV  +       L
Sbjct: 6   LLLALLLAVSAAAAAQVGGNRGHGPLVGGWSPITDVGDPHIQELGGWAVERHASLSSDGL 65

Query: 69  KFVKVVKGESQVVSGTNYRLFIEADD----NSQYVAIVYDVPWRHQRSLTSFTPAA 117
           +F +V  GE QVVSG NYRL + A D     + YVA+VY+  W + R LTSF PAA
Sbjct: 66  RFRRVTSGEQQVVSGMNYRLVVSASDPAGATASYVAVVYEQSWTNTRQLTSFKPAA 121
[Close]
BLAST of Spo20170.1 vs. ExPASy Swiss-Prot
Match: CYT1_ACTDE (Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa PE=1 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 3.800e-17
Identity = 52/108 (48.15%), Postives = 69/108 (63.89%), Query Frame = 1 

		  

Query: 8   LLALSLLAVVSAVSASRKHGGALVGGYSPIKDVSDPYVQGVGQFAVTEYNKDQKAHLKFV 67
           LL   LLA+ +AV   RK   A  GG+ PI+ ++   VQ V QFAV+E+NK     L++ 
Sbjct: 8   LLLFLLLALSAAVVGGRKLVAA--GGWRPIESLNSAEVQDVAQFAVSEHNKQANDELQYQ 67

Query: 68  KVVKGESQVVSGTNYRLFIEADDNS---QYVAIVYDVPWRHQRSLTSF 113
            VV+G +QVV+GTNYRL I A D +    Y A+V+D PW H R+LTSF
Sbjct: 68  SVVRGYTQVVAGTNYRLVIAAKDGAVVGNYEAVVWDKPWMHFRNLTSF 113
[Close]
BLAST of Spo20170.1 vs. ExPASy Swiss-Prot
Match: CYT4_ARATH (Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana GN=CYS4 PE=3 SV=2)

HSP 1 Score: 84.3 bits (207), Expect = 9.300e-16
Identity = 52/116 (44.83%), Postives = 68/116 (58.62%), Query Frame = 1 

		  

Query: 1   MSSRHFLLLALSLLAVVSAVSASRKHGGALVGGYSPIKDVSDPYVQGVGQFAVTEYNKDQ 60
           M  +  + L+L LL +VS V      GG  +G   PIK+VSDP V  V ++A+ E+NK+ 
Sbjct: 1   MMMKSLICLSLILLPLVSVVEGLGGGGG--LGSRKPIKNVSDPDVVAVAKYAIEEHNKES 60

Query: 61  KAHLKFVKVVKGESQVVSGTNYRLFIEADDN----SQYVAIVYDVPWRHQRSLTSF 113
           K  L FVKVV+G +QVVSGT Y L I A D       Y A+V +  W H +SL SF
Sbjct: 61  KEKLVFVKVVEGTTQVVSGTKYDLKIAAKDGGGKIKNYEAVVVEKLWLHSKSLESF 114
[Close]
BLAST of Spo20170.1 vs. ExPASy Swiss-Prot
Match: CYT6_ORYSJ (Cysteine proteinase inhibitor 6 OS=Oryza sativa subsp. japonica GN=Os03g0210200 PE=3 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 9.600e-13
Identity = 46/106 (43.40%), Postives = 62/106 (58.49%), Query Frame = 1 

		  

Query: 13  LLAVVSAVSASRKHGGALVGGYSPIKDVSDPYVQGVGQFAVTEYNK-DQKAHLKFVKVVK 72
           LLA V A +A       L  G+SPIK++ DP++Q +G++A+TE N+      L F +V  
Sbjct: 9   LLAAVCAAAA-------LPRGWSPIKNIDDPHIQELGRWAITENNRVSPSDELTFHRVTG 68

Query: 73  GESQVVSGTNYRLFIEA-----DDNSQYVAIVYDVPWRHQRSLTSF 113
           GE QVVSG NYRL IEA     D    Y A+V++  W + R L SF
Sbjct: 69  GEQQVVSGMNYRLEIEAASGGGDVTGSYGAVVFEQEWSNTRKLISF 107
[Close]
BLAST of Spo20170.1 vs. TAIR (Arabidopsis)
Match: AT5G47550.1 (Cystatin/monellin superfamily protein)

HSP 1 Score: 107.8 bits (268), Expect = 4.400e-24
Identity = 62/119 (52.10%), Postives = 82/119 (68.91%), Query Frame = 1 

		  

Query: 1   MSSRHFLLLALSLLAVVSAVSASRKHGGALVGGYSPIKDVSDPYVQGVGQFAVTEYNKDQ 60
           M+S+   LL LSL+ V+  + AS     A VGG+SPI +V+DP V  +G+FAV+EYNK  
Sbjct: 1   MTSKVVFLLLLSLVVVLLPLYAS---AAARVGGWSPISNVTDPQVVEIGEFAVSEYNKRS 60

Query: 61  KAHLKFVKVVKGESQVVSGTNYRLFIEADD----NSQYVAIVYDVPWRHQRSLTSFTPA 116
           ++ LKF  VV GE+QVVSGTNYRL + A+D    +  Y+AIV+D PW   R+LTSF PA
Sbjct: 61  ESGLKFETVVSGETQVVSGTNYRLKVAANDGDGVSKNYLAIVWDKPWMKFRNLTSFEPA 116
[Close]
BLAST of Spo20170.1 vs. TAIR (Arabidopsis)
Match: AT4G16500.1 (Cystatin/monellin superfamily protein)

HSP 1 Score: 84.3 bits (207), Expect = 5.200e-17
Identity = 52/116 (44.83%), Postives = 68/116 (58.62%), Query Frame = 1 

		  

Query: 1   MSSRHFLLLALSLLAVVSAVSASRKHGGALVGGYSPIKDVSDPYVQGVGQFAVTEYNKDQ 60
           M  +  + L+L LL +VS V      GG  +G   PIK+VSDP V  V ++A+ E+NK+ 
Sbjct: 1   MMMKSLICLSLILLPLVSVVEGLGGGGG--LGSRKPIKNVSDPDVVAVAKYAIEEHNKES 60

Query: 61  KAHLKFVKVVKGESQVVSGTNYRLFIEADDN----SQYVAIVYDVPWRHQRSLTSF 113
           K  L FVKVV+G +QVVSGT Y L I A D       Y A+V +  W H +SL SF
Sbjct: 61  KEKLVFVKVVEGTTQVVSGTKYDLKIAAKDGGGKIKNYEAVVVEKLWLHSKSLESF 114
[Close]
BLAST of Spo20170.1 vs. TAIR (Arabidopsis)
Match: AT3G12490.2 (cystatin B)

HSP 1 Score: 57.8 bits (138), Expect = 5.300e-9
Identity = 38/115 (33.04%), Postives = 63/115 (54.78%), Query Frame = 1 

		  

Query: 6   FLLLALSLLAVVSAVSASRKHGGALVGGYSPIK-DVSDPYVQGVGQFAVTEYNKDQKAHL 65
           F  L+L + +++++         ALVGG   +  + +   V+ + +FAV E+NK + A L
Sbjct: 12  FFSLSLFISSLIASDLGFCNEEMALVGGVGDVPANQNSGEVESLARFAVDEHNKKENALL 71

Query: 66  KFVKVVKGESQVVSGTNYRL---FIEADDNSQYVAIVYDVPWRHQRSLTSFTPAA 117
           +F +VVK + QVV+GT + L    +EA     Y A V+  PW + + L  F PA+
Sbjct: 72  EFARVVKAKEQVVAGTLHHLTLEILEAGQKKLYEAKVWVKPWLNFKELQEFKPAS 126
[Close]
BLAST of Spo20170.1 vs. TAIR (Arabidopsis)
Match: AT5G12140.1 (cystatin-1)

HSP 1 Score: 54.3 bits (129), Expect = 5.800e-8
Identity = 30/92 (32.61%), Postives = 53/92 (57.61%), Query Frame = 1 

		  

Query: 25  KHGGALVGGYSPIK-DVSDPYVQGVGQFAVTEYNKDQKAHLKFVKVVKGESQVVSGTNYR 84
           +  G +VGG   I  + +D  V+ + +FAV E+NK++   L++ +++  ++QVV+GT + 
Sbjct: 4   QQAGTIVGGVRDIDANANDLQVESLARFAVDEHNKNENLTLEYKRLLGAKTQVVAGTMHH 63

Query: 85  LFIEADD---NSQYVAIVYDVPWRHQRSLTSF 113
           L +E  D   N  Y A V +  W + + L SF
Sbjct: 64  LTVEVADGETNKVYEAKVLEKAWENLKQLESF 95
[Close]
BLAST of Spo20170.1 vs. TAIR (Arabidopsis)
Match: AT2G40880.1 (cystatin A)

HSP 1 Score: 53.1 bits (126), Expect = 1.300e-7
Identity = 36/120 (30.00%), Postives = 62/120 (51.67%), Query Frame = 1 

		  

Query: 1   MSSRHFLLLALSLLAVVS-AVSASRKHGGA---LVGGYSPIK-DVSDPYVQGVGQFAVTE 60
           M S+ F ++ L L   +  A+  S +       ++GG   ++ + +   ++ + +FA+ E
Sbjct: 1   MESKTFWIVTLLLCGTIQLAICRSEEKSTEKTMMLGGVHDLRGNQNSGEIESLARFAIQE 60

Query: 61  YNKDQKAHLKFVKVVKGESQVVSGTNYRLFIEA---DDNSQYVAIVYDVPWRHQRSLTSF 113
           +NK Q   L+F K+VK   QVV+GT Y L +EA   D    + A V+  PW + + L  F
Sbjct: 61  HNKQQNKILEFKKIVKAREQVVAGTMYHLTLEAKEGDQTKNFEAKVWVKPWMNFKQLQEF 120
The following BLAST results are available for this feature:
BLAST of Spo20170.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902233059|gb|KNA22917.1|6.4e-57100.hypothetical protein SOVF_0296... [more]
gi|731350829|ref|XP_010686713.1|4.9e-3366.0PREDICTED: cysteine proteinase... [more]
gi|703092669|ref|XP_010094696.1|2.1e-2353.0Cysteine proteinase inhibitor ... [more]
gi|746657137|gb|AJD79055.1|2.7e-2353.0CPI-4 [Morus alba var. atropur... [more]
gi|388496176|gb|AFK36154.1|5.1e-2250.8unknown [Lotus japonicus][more]
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BLAST of Spo20170.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9RVP8_SPIOL4.4e-57100.Cysteine proteinase inhibitor ... [more]
A0A0J8EMH9_BETVU3.4e-3366.0Cysteine proteinase inhibitor ... [more]
W9R7F6_9ROSA1.4e-2353.0Cysteine proteinase inhibitor ... [more]
A0A0B4ZWL9_MORAL1.9e-2353.0Cysteine proteinase inhibitor ... [more]
I3S7B2_LOTJA3.6e-2250.8Cysteine proteinase inhibitor ... [more]
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BLAST of Spo20170.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5
Match NameE-valueIdentityDescription
CYT5_ARATH7.9e-2352.1Cysteine proteinase inhibitor ... [more]
CYT8_ORYSJ2.4e-1947.4Cysteine proteinase inhibitor ... [more]
CYT1_ACTDE3.8e-1748.1Cysteine proteinase inhibitor ... [more]
CYT4_ARATH9.3e-1644.8Cysteine proteinase inhibitor ... [more]
CYT6_ORYSJ9.6e-1343.4Cysteine proteinase inhibitor ... [more]
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BLAST of Spo20170.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5
Match NameE-valueIdentityDescription
AT5G47550.14.4e-2452.1Cystatin/monellin superfamily ... [more]
AT4G16500.15.2e-1744.8Cystatin/monellin superfamily ... [more]
AT3G12490.25.3e-933.0cystatin B[more]
AT5G12140.15.8e-832.6cystatin-1[more]
AT2G40880.11.3e-730.0cystatin A[more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000010Cystatin domainSMARTSM00043CY_4coord: 29..115
score: 1.6
IPR018073Proteinase inhibitor I25, cystatin, conserved sitePROSITEPS00287CYSTATINcoord: 74..87
scor
IPR020381Proteinase inhibitor I25, cystatin, conserved regionPRODOMPD001231coord: 48..115
score: 1.0
IPR020381Proteinase inhibitor I25, cystatin, conserved regionPFAMPF16845SQAPIcoord: 37..114
score: 5.5
IPR027214CystatinPANTHERPTHR11413CYSTATIN FAMILY MEMBERcoord: 7..116
score: 6.6
NoneNo IPR availableGENE3D3.10.450.10coord: 30..115
score: 2.6
NoneNo IPR availablePANTHERPTHR11413:SF59SUBFAMILY NOT NAMEDcoord: 7..116
score: 6.6
NoneNo IPR availableunknownSSF54403Cystatin/monellincoord: 29..114
score: 8.93

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010466 negative regulation of peptidase activity
biological_process GO:0009423 chorismate biosynthetic process
biological_process GO:0008152 metabolic process
biological_process GO:0000162 tryptophan biosynthetic process
biological_process GO:0030001 metal ion transport
biological_process GO:0006354 DNA-templated transcription, elongation
biological_process GO:0042254 ribosome biogenesis
biological_process GO:0006412 translation
biological_process GO:0006571 tyrosine biosynthetic process
biological_process GO:0006744 ubiquinone biosynthetic process
biological_process GO:0006084 acetyl-CoA metabolic process
biological_process GO:0015991 ATP hydrolysis coupled proton transport
biological_process GO:0019722 calcium-mediated signaling
biological_process GO:0005513 detection of calcium ion
biological_process GO:0048193 Golgi vesicle transport
biological_process GO:0010099 regulation of photomorphogenesis
biological_process GO:0009612 response to mechanical stimulus
biological_process GO:0006694 steroid biosynthetic process
biological_process GO:0008299 isoprenoid biosynthetic process
biological_process GO:0015936 coenzyme A metabolic process
biological_process GO:0009987 cellular process
biological_process GO:0033587 shikimate biosynthetic process
biological_process GO:0009094 L-phenylalanine biosynthetic process
biological_process GO:0006468 protein phosphorylation
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0009407 toxin catabolic process
biological_process GO:0009651 response to salt stress
biological_process GO:0008643 carbohydrate transport
biological_process GO:0055085 transmembrane transport
biological_process GO:0051788 response to misfolded protein
biological_process GO:0046686 response to cadmium ion
biological_process GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process
biological_process GO:0006397 mRNA processing
biological_process GO:0016120 carotene biosynthetic process
biological_process GO:0009765 photosynthesis, light harvesting
biological_process GO:0010103 stomatal complex morphogenesis
biological_process GO:0016123 xanthophyll biosynthetic process
biological_process GO:0080129 proteasome core complex assembly
biological_process GO:0006096 glycolytic process
biological_process GO:0009069 serine family amino acid metabolic process
biological_process GO:0006007 glucose catabolic process
biological_process GO:0006334 nucleosome assembly
biological_process GO:0006605 protein targeting
biological_process GO:0006094 gluconeogenesis
biological_process GO:0006635 fatty acid beta-oxidation
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0009570 chloroplast stroma
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0005839 proteasome core complex
cellular_component GO:0005667 transcription factor complex
cellular_component GO:0009706 chloroplast inner membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0031969 chloroplast membrane
cellular_component GO:0000786 nucleosome
cellular_component GO:0005634 nucleus
cellular_component GO:0005618 cell wall
cellular_component GO:0031410 cytoplasmic vesicle
cellular_component GO:0033179 proton-transporting V-type ATPase, V0 domain
cellular_component GO:0005774 vacuolar membrane
cellular_component GO:0005576 extracellular region
cellular_component GO:0009507 chloroplast
cellular_component GO:0005840 ribosome
cellular_component GO:0005829 cytosol
cellular_component GO:0005802 trans-Golgi network
cellular_component GO:0005768 endosome
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0051287 NAD binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0042802 identical protein binding
molecular_function GO:0003856 3-dehydroquinate synthase activity
molecular_function GO:0005515 protein binding
molecular_function GO:0004298 threonine-type endopeptidase activity
molecular_function GO:0015078 proton transmembrane transporter activity
molecular_function GO:0004869 cysteine-type endopeptidase inhibitor activity
molecular_function GO:0005351 carbohydrate:proton symporter activity
molecular_function GO:0045435 lycopene epsilon cyclase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function GO:0003677 DNA binding
molecular_function GO:0046982 protein heterodimerization activity
molecular_function GO:0003735 structural constituent of ribosome
molecular_function GO:0017111 nucleoside-triphosphatase activity
molecular_function GO:0032440 2-alkenal reductase [NAD(P)] activity
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0004420 hydroxymethylglutaryl-CoA reductase (NADPH) activity
molecular_function GO:0050661 NADP binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0005524 ATP binding
molecular_function GO:0000166 nucleotide binding
RNA-Seq Expression