Spo20868 (gene)

Overview
NameSpo20868
Typegene
OrganismSpinacia oleracea (Spinach)
DescriptionrRNA N-glycosidase (3.2.2.22)
LocationSpoScf_22195 : 732 .. 1196 (-)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTCCAGAGGTACCTGCAGGTGCTAATCGTCTAACAATTAAATACAAAAGTGCATATTTGGATATTGAGAAGAAGGGTGGAAGTAGAACCAAACTCGGATTGGGTGTTAAACAAGTTAACAAGTTTATCAAAAATGTTTACGGGAAGCCAATTGACGAGAAAACTATTCCGATCCAAGCTCAGTTTATGCTTATTGTCATCCAAACGATAGCAGAGGCAACACGATTCAAGTATATCGAGAACTTGATTCTGGCTGATTTCGATTCATTTGAAAATAGGCCCGACCCTAAAACGATTGAATTGGAAAAGAGTTGGCAGAAAATCACCATAGGAATTAAGACCTCTACTAAGAAAGGGGAGATCAAGCCAGCGTTGGATTTAACGTATGATAAAGATAAGCCTTGGATTGTGTCGGAAGTCAGTCAAATTGCTCCAGACATGGGGCTTCTAAAGTACGAAGGC

mRNA sequence

ATGTTTCCAGAGGTACCTGCAGGTGCTAATCGTCTAACAATTAAATACAAAAGTGCATATTTGGATATTGAGAAGAAGGGTGGAAGTAGAACCAAACTCGGATTGGGTGTTAAACAAGTTAACAAGTTTATCAAAAATGTTTACGGGAAGCCAATTGACGAGAAAACTATTCCGATCCAAGCTCAGTTTATGCTTATTGTCATCCAAACGATAGCAGAGGCAACACGATTCAAGTATATCGAGAACTTGATTCTGGCTGATTTCGATTCATTTGAAAATAGGCCCGACCCTAAAACGATTGAATTGGAAAAGAGTTGGCAGAAAATCACCATAGGAATTAAGACCTCTACTAAGAAAGGGGAGATCAAGCCAGCGTTGGATTTAACGTATGATAAAGATAAGCCTTGGATTGTGTCGGAAGTCAGTCAAATTGCTCCAGACATGGGGCTTCTAAAGTACGAAGGC

Coding sequence (CDS)

ATGTTTCCAGAGGTACCTGCAGGTGCTAATCGTCTAACAATTAAATACAAAAGTGCATATTTGGATATTGAGAAGAAGGGTGGAAGTAGAACCAAACTCGGATTGGGTGTTAAACAAGTTAACAAGTTTATCAAAAATGTTTACGGGAAGCCAATTGACGAGAAAACTATTCCGATCCAAGCTCAGTTTATGCTTATTGTCATCCAAACGATAGCAGAGGCAACACGATTCAAGTATATCGAGAACTTGATTCTGGCTGATTTCGATTCATTTGAAAATAGGCCCGACCCTAAAACGATTGAATTGGAAAAGAGTTGGCAGAAAATCACCATAGGAATTAAGACCTCTACTAAGAAAGGGGAGATCAAGCCAGCGTTGGATTTAACGTATGATAAAGATAAGCCTTGGATTGTGTCGGAAGTCAGTCAAATTGCTCCAGACATGGGGCTTCTAAAGTACGAAGGC

Protein sequence

MFPEVPAGANRLTIKYKSAYLDIEKKGGSRTKLGLGVKQVNKFIKNVYGKPIDEKTIPIQAQFMLIVIQTIAEATRFKYIENLILADFDSFENRPDPKTIELEKSWQKITIGIKTSTKKGEIKPALDLTYDKDKPWIVSEVSQIAPDMGLLKYEG
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo20868.1Spo20868.1mRNA


Homology
[Close]
BLAST of Spo20868.1 vs. NCBI nr
Match: gi|902225392|gb|KNA20052.1| (hypothetical protein SOVF_055950 [Spinacia oleracea])

HSP 1 Score: 220.3 bits (560), Expect = 2.300e-54
Identity = 116/153 (75.82%), Postives = 126/153 (82.35%), Query Frame = 1 

		  

Query: 4   EVPAGAN-RLTIKYKSAYLDIEKKGGSRTKLGLGVKQVNKFIKNVYGKPIDEKTIPIQAQ 63
           +VP G   RL +KYKS Y DIEK GGSRTKLGLGVKQ+NKFI NVYGKP D KT   +AQ
Sbjct: 138 QVPVGNKYRLPMKYKSTYGDIEKNGGSRTKLGLGVKQLNKFINNVYGKPFDAKT---EAQ 197

Query: 64  FMLIVIQTIAEATRFKYIENLILADFDSFENRPDPKTIELEKSWQKITIGIKTSTKKGEI 123
           FMLIVIQTIAEATRFKYIEN+ILA FDSFEN+PDPKTIELE SWQKIT GIK+S  +GEI
Sbjct: 198 FMLIVIQTIAEATRFKYIENMILAKFDSFENKPDPKTIELEVSWQKITKGIKSSNAEGEI 257

Query: 124 KPALDLTYDKDKPWIVSEVSQIAPDMGLLKYEG 156
           KP L L   K K W V++VSQIAPDMGLLKYEG
Sbjct: 258 KPELILKDAKGKTWKVNKVSQIAPDMGLLKYEG 287
[Close]
BLAST of Spo20868.1 vs. NCBI nr
Match: gi|731354828|ref|XP_010688810.1| (PREDICTED: protein synthesis inhibitor PD-S2-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 139.4 bits (350), Expect = 5.200e-30
Identity = 79/157 (50.32%), Postives = 102/157 (64.97%), Query Frame = 1 

		  

Query: 1   MFPEVPAGANRLTIKYKSAYLDIEKKG-GSRTKLGLGVKQVNKFIKNVYGKPIDEKTIPI 60
           +FPE      RL + YK  Y ++EK     R+K+GLG + + K +  VYGK +D   +P 
Sbjct: 121 LFPEAKGAKYRLQMNYKGTYGELEKNAKADRSKVGLGKQPLKKSMDQVYGKKLD---VPT 180

Query: 61  QAQFMLIVIQTIAEATRFKYIENLILAD-FDSFENRPDPKTIELEKSWQKITIGIKTSTK 120
           +A+FML+ IQ  AEATRFKYIEN I+   FD   N PD K+I+ E SWQKIT GIK S+K
Sbjct: 181 EAKFMLVAIQMFAEATRFKYIENKIIGQKFDQVYN-PDHKSIKFELSWQKITKGIK-SSK 240

Query: 121 KGEIKPALDLTYDKDKPWIVSEVSQIAPDMGLLKYEG 156
            G I P +DL   ++KPW VS+VS +  DMGLLK EG
Sbjct: 241 NGVISPPIDLVDAQNKPWKVSKVSDVINDMGLLKNEG 272
[Close]
BLAST of Spo20868.1 vs. NCBI nr
Match: gi|870850466|gb|KMT02566.1| (hypothetical protein BVRB_9g202530 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 139.4 bits (350), Expect = 5.200e-30
Identity = 79/157 (50.32%), Postives = 102/157 (64.97%), Query Frame = 1 

		  

Query: 1   MFPEVPAGANRLTIKYKSAYLDIEKKG-GSRTKLGLGVKQVNKFIKNVYGKPIDEKTIPI 60
           +FPE      RL + YK  Y ++EK     R+K+GLG + + K +  VYGK +D   +P 
Sbjct: 124 LFPEAKGAKYRLQMNYKGTYGELEKNAKADRSKVGLGKQPLKKSMDQVYGKKLD---VPT 183

Query: 61  QAQFMLIVIQTIAEATRFKYIENLILAD-FDSFENRPDPKTIELEKSWQKITIGIKTSTK 120
           +A+FML+ IQ  AEATRFKYIEN I+   FD   N PD K+I+ E SWQKIT GIK S+K
Sbjct: 184 EAKFMLVAIQMFAEATRFKYIENKIIGQKFDQVYN-PDHKSIKFELSWQKITKGIK-SSK 243

Query: 121 KGEIKPALDLTYDKDKPWIVSEVSQIAPDMGLLKYEG 156
            G I P +DL   ++KPW VS+VS +  DMGLLK EG
Sbjct: 244 NGVISPPIDLVDAQNKPWKVSKVSDVINDMGLLKNEG 275
[Close]
BLAST of Spo20868.1 vs. NCBI nr
Match: gi|99646720|emb|CAK22418.1| (ribosome-inactivating protein [Beta vulgaris])

HSP 1 Score: 133.3 bits (334), Expect = 3.700e-28
Identity = 72/155 (46.45%), Postives = 96/155 (61.94%), Query Frame = 1 

		  

Query: 1   MFPEVPAGANRLTIKYKSAYLDIEKKGGSRTKLGLGVKQVNKFIKNVYGKPIDEKTIPIQ 60
           +FP      NR+ + YK +Y DIEK    R+K+ LG+ ++ K +  VYG+   +  +P +
Sbjct: 120 LFPNARGANNRIIMFYKGSYPDIEKVAKDRSKISLGIYELRKLMIQVYGQ--SKVDVPTE 179

Query: 61  AQFMLIVIQTIAEATRFKYIENLILADFDSFENRPDPKTIELEKSWQKITIGIKTSTKKG 120
           A+ ML+ IQ I+EA RFKYIEN IL  F     +PDPK ++LE SWQ IT+GI+ S + G
Sbjct: 180 AKLMLLFIQMISEAARFKYIENKILETF-YMSFKPDPKILKLETSWQAITVGIRNS-RNG 239

Query: 121 EIKPALDLTYDKDKPWIVSEVSQIAPDMGLLKYEG 156
            I PAL L     + WIVS V  I  DM LLKYEG
Sbjct: 240 VISPALKLDDVNGRDWIVSRVQDIVDDMALLKYEG 270
[Close]
BLAST of Spo20868.1 vs. NCBI nr
Match: gi|731354498|ref|XP_010688629.1| (PREDICTED: antiviral protein I-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 132.5 bits (332), Expect = 6.400e-28
Identity = 72/155 (46.45%), Postives = 96/155 (61.94%), Query Frame = 1 

		  

Query: 1   MFPEVPAGANRLTIKYKSAYLDIEKKGGSRTKLGLGVKQVNKFIKNVYGKPIDEKTIPIQ 60
           +FP      NR+ + YK +Y DIEK    R+K+ LG+ ++ K +  VYG+   +  +P +
Sbjct: 124 LFPNARGTNNRIIMFYKGSYPDIEKVAKDRSKISLGIYELRKLMIQVYGQ--SKVDVPTE 183

Query: 61  AQFMLIVIQTIAEATRFKYIENLILADFDSFENRPDPKTIELEKSWQKITIGIKTSTKKG 120
           A+ ML+ IQ I+EA RFKYIEN IL  F     +PDPK ++LE SWQ IT+GI+ S + G
Sbjct: 184 AKLMLLFIQMISEAARFKYIENKILETF-YMSFKPDPKILKLETSWQAITVGIRNS-RNG 243

Query: 121 EIKPALDLTYDKDKPWIVSEVSQIAPDMGLLKYEG 156
            I PAL L     + WIVS V  I  DM LLKYEG
Sbjct: 244 VISPALKLDDVNGRDWIVSRVQDIVDDMALLKYEG 274
[Close]
BLAST of Spo20868.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RMG6_SPIOL (rRNA N-glycosidase OS=Spinacia oleracea GN=SOVF_055950 PE=3 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 1.600e-54
Identity = 116/153 (75.82%), Postives = 126/153 (82.35%), Query Frame = 1 

		  

Query: 4   EVPAGAN-RLTIKYKSAYLDIEKKGGSRTKLGLGVKQVNKFIKNVYGKPIDEKTIPIQAQ 63
           +VP G   RL +KYKS Y DIEK GGSRTKLGLGVKQ+NKFI NVYGKP D KT   +AQ
Sbjct: 138 QVPVGNKYRLPMKYKSTYGDIEKNGGSRTKLGLGVKQLNKFINNVYGKPFDAKT---EAQ 197

Query: 64  FMLIVIQTIAEATRFKYIENLILADFDSFENRPDPKTIELEKSWQKITIGIKTSTKKGEI 123
           FMLIVIQTIAEATRFKYIEN+ILA FDSFEN+PDPKTIELE SWQKIT GIK+S  +GEI
Sbjct: 198 FMLIVIQTIAEATRFKYIENMILAKFDSFENKPDPKTIELEVSWQKITKGIKSSNAEGEI 257

Query: 124 KPALDLTYDKDKPWIVSEVSQIAPDMGLLKYEG 156
           KP L L   K K W V++VSQIAPDMGLLKYEG
Sbjct: 258 KPELILKDAKGKTWKVNKVSQIAPDMGLLKYEG 287
[Close]
BLAST of Spo20868.1 vs. UniProtKB/TrEMBL
Match: A0A0J8EHV6_BETVU (rRNA N-glycosidase OS=Beta vulgaris subsp. vulgaris GN=BVRB_9g202530 PE=3 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 3.600e-30
Identity = 79/157 (50.32%), Postives = 102/157 (64.97%), Query Frame = 1 

		  

Query: 1   MFPEVPAGANRLTIKYKSAYLDIEKKG-GSRTKLGLGVKQVNKFIKNVYGKPIDEKTIPI 60
           +FPE      RL + YK  Y ++EK     R+K+GLG + + K +  VYGK +D   +P 
Sbjct: 124 LFPEAKGAKYRLQMNYKGTYGELEKNAKADRSKVGLGKQPLKKSMDQVYGKKLD---VPT 183

Query: 61  QAQFMLIVIQTIAEATRFKYIENLILAD-FDSFENRPDPKTIELEKSWQKITIGIKTSTK 120
           +A+FML+ IQ  AEATRFKYIEN I+   FD   N PD K+I+ E SWQKIT GIK S+K
Sbjct: 184 EAKFMLVAIQMFAEATRFKYIENKIIGQKFDQVYN-PDHKSIKFELSWQKITKGIK-SSK 243

Query: 121 KGEIKPALDLTYDKDKPWIVSEVSQIAPDMGLLKYEG 156
            G I P +DL   ++KPW VS+VS +  DMGLLK EG
Sbjct: 244 NGVISPPIDLVDAQNKPWKVSKVSDVINDMGLLKNEG 275
[Close]
BLAST of Spo20868.1 vs. UniProtKB/TrEMBL
Match: Q1H8M9_BETVU (rRNA N-glycosidase OS=Beta vulgaris GN=rip-r2 PE=2 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 2.600e-28
Identity = 72/155 (46.45%), Postives = 96/155 (61.94%), Query Frame = 1 

		  

Query: 1   MFPEVPAGANRLTIKYKSAYLDIEKKGGSRTKLGLGVKQVNKFIKNVYGKPIDEKTIPIQ 60
           +FP      NR+ + YK +Y DIEK    R+K+ LG+ ++ K +  VYG+   +  +P +
Sbjct: 120 LFPNARGANNRIIMFYKGSYPDIEKVAKDRSKISLGIYELRKLMIQVYGQ--SKVDVPTE 179

Query: 61  AQFMLIVIQTIAEATRFKYIENLILADFDSFENRPDPKTIELEKSWQKITIGIKTSTKKG 120
           A+ ML+ IQ I+EA RFKYIEN IL  F     +PDPK ++LE SWQ IT+GI+ S + G
Sbjct: 180 AKLMLLFIQMISEAARFKYIENKILETF-YMSFKPDPKILKLETSWQAITVGIRNS-RNG 239

Query: 121 EIKPALDLTYDKDKPWIVSEVSQIAPDMGLLKYEG 156
            I PAL L     + WIVS V  I  DM LLKYEG
Sbjct: 240 VISPALKLDDVNGRDWIVSRVQDIVDDMALLKYEG 270
[Close]
BLAST of Spo20868.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BRF2_BETVU (rRNA N-glycosidase OS=Beta vulgaris subsp. vulgaris GN=BVRB_9g202460 PE=3 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 4.400e-28
Identity = 72/155 (46.45%), Postives = 96/155 (61.94%), Query Frame = 1 

		  

Query: 1   MFPEVPAGANRLTIKYKSAYLDIEKKGGSRTKLGLGVKQVNKFIKNVYGKPIDEKTIPIQ 60
           +FP      NR+ + YK +Y DIEK    R+K+ LG+ ++ K +  VYG+   +  +P +
Sbjct: 124 LFPNARGTNNRIIMFYKGSYPDIEKVAKDRSKISLGIYELRKLMIQVYGQ--SKVDVPTE 183

Query: 61  AQFMLIVIQTIAEATRFKYIENLILADFDSFENRPDPKTIELEKSWQKITIGIKTSTKKG 120
           A+ ML+ IQ I+EA RFKYIEN IL  F     +PDPK ++LE SWQ IT+GI+ S + G
Sbjct: 184 AKLMLLFIQMISEAARFKYIENKILETF-YMSFKPDPKILKLETSWQAITVGIRNS-RNG 243

Query: 121 EIKPALDLTYDKDKPWIVSEVSQIAPDMGLLKYEG 156
            I PAL L     + WIVS V  I  DM LLKYEG
Sbjct: 244 VISPALKLDDVNGRDWIVSRVQDIVDDMALLKYEG 274
[Close]
BLAST of Spo20868.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BMU1_BETVU (rRNA N-glycosidase OS=Beta vulgaris subsp. vulgaris GN=BVRB_9g202490 PE=3 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 3.800e-27
Identity = 75/158 (47.47%), Postives = 98/158 (62.03%), Query Frame = 1 

		  

Query: 1   MFPEVPAGANRLTIKYKSAYLDIEKKG-GSRTKLGLGVKQVNKFIKNVYGKPIDEK--TI 60
           +FPE     NRL + Y + Y  IE K    R+ LGLGV+++   +  VYG  +D+K    
Sbjct: 121 LFPEAKGDKNRLDMHYANTYDAIEGKAEAKRSNLGLGVEKLKVLVNYVYGLKVDKKKGVD 180

Query: 61  PIQAQFMLIVIQTIAEATRFKYIENLILADFDSFENRPDPKTIELEKSWQKITIGIKTST 120
           P +A+ ML++IQ ++EA RFKYIE++IL +FD+    PDPK   LE  W KI+ GIK S 
Sbjct: 181 PKEAKLMLVLIQMVSEAARFKYIEDMILDNFDT-SIIPDPKATRLENRWGKISEGIKNS- 240

Query: 121 KKGEIKPALDLTYDKDKPWIVSEVSQIAPDMGLLKYEG 156
           KKG I P LDL   + K W VS+V  I  DMGLLKY G
Sbjct: 241 KKGVISPELDLLDAQGKAWKVSKVKDIEGDMGLLKYSG 276
[Close]
BLAST of Spo20868.1 vs. ExPASy Swiss-Prot
Match: RIPL2_PHYDI (Ribosome-inactivating protein PD-L3/PD-L4 OS=Phytolacca dioica PE=1 SV=2)

HSP 1 Score: 99.0 bits (245), Expect = 4.900e-20
Identity = 56/142 (39.44%), Postives = 85/142 (59.86%), Query Frame = 1 

		  

Query: 14  IKYKSAYLDIEKKGG--SRTKLGLGVKQVNKFIKNVYGKPIDEKTIPIQAQFMLIVIQTI 73
           I Y S Y  ++ K G  SR+++ LG++ +N  I  + G  +   T   +A+F+L+ IQ +
Sbjct: 116 INYNSQYSTLQNKAGVSSRSQVQLGIQILNSDIGKISG--VSTFTDKTEAEFLLVAIQMV 175

Query: 74  AEATRFKYIENLILADFDSFENRPDPKTIELEKSWQKITIGIKTSTKKGEIKPALDLTYD 133
           +EA RFKYIEN +  +F+   N P+PK + LE++W KI++ I  + K G +   L+L   
Sbjct: 176 SEAARFKYIENQVKTNFNRAFN-PNPKVLSLEENWGKISLAIH-NAKNGALTSPLELKNA 235

Query: 134 KDKPWIVSEVSQIAPDMGLLKY 154
            D  WIV  V +I PDMGLL Y
Sbjct: 236 DDTKWIVLRVDEIKPDMGLLNY 253
[Close]
BLAST of Spo20868.1 vs. ExPASy Swiss-Prot
Match: RIPD2_PHYDI (Dioicin-2 OS=Phytolacca dioica PE=1 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 1.400e-19
Identity = 62/155 (40.00%), Postives = 85/155 (54.84%), Query Frame = 1 

		  

Query: 5   VPAGANRLTIKYKSAYLDIEKKGGSRTKLGLGVKQVNKFIKNVYGK-PIDEKTI-PIQAQ 64
           +P   N+  I YK +Y  +E KGG RTKLGLG   +   +  +YGK   D+K     +A+
Sbjct: 108 LPGSQNK--IPYKKSYQSMESKGGDRTKLGLGQITLESRMNKIYGKDATDQKQFQKNEAE 167

Query: 65  FMLIVIQTIAEATRFKYIENLILADF-DSFENRPDPKTIELEKSWQKITIGIKTSTKKGE 124
           F+LI +Q I EA+RFKYIEN +   F D+   +PDPK I LE SW KI+  I      G 
Sbjct: 168 FLLIAVQMITEASRFKYIENKVKDSFDDAIGYKPDPKAISLETSWDKISNAIAKVNTPGN 227

Query: 125 IKPAL--DLTYDKDKPWIVSEVSQIAPD-MGLLKY 154
               L   L  +  KPW  + + ++  D MGLL +
Sbjct: 228 SIVTLPKGLLDENKKPWTTATMDELKNDIMGLLTH 260
[Close]
BLAST of Spo20868.1 vs. ExPASy Swiss-Prot
Match: RIPA_PHYAM (Antiviral protein alpha OS=Phytolacca americana PE=1 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 1.400e-19
Identity = 57/141 (40.43%), Postives = 84/141 (59.57%), Query Frame = 1 

		  

Query: 14  IKYKSAYLDIEKK-GGSRTKLGLGVKQVNKFIKNVYGKPIDEKTIPIQAQFMLIVIQTIA 73
           I Y S+Y  +EKK G  R+++ LG++ +N  I  +YG  +D  T   +A+F+L+ IQ ++
Sbjct: 141 INYDSSYPALEKKVGRPRSQVQLGIQILNSGIGKIYG--VDSFTEKTEAEFLLVAIQMVS 200

Query: 74  EATRFKYIENLILADFDSFENRPDPKTIELEKSWQKITIGIKTSTKKGEIKPALDLTYDK 133
           EA RFKYIEN +  +F+     P+ K + LE+SW KI+  I  + K G +   L+L    
Sbjct: 201 EAARFKYIENQVKTNFNR-AFYPNAKVLNLEESWGKISTAIH-NAKNGALTSPLELKNAN 260

Query: 134 DKPWIVSEVSQIAPDMGLLKY 154
              WIV  V  I PD+GLLKY
Sbjct: 261 GSKWIVLRVDDIEPDVGLLKY 277
[Close]
BLAST of Spo20868.1 vs. ExPASy Swiss-Prot
Match: RIP2_PHYAM (Antiviral protein 2 OS=Phytolacca americana GN=PAP2 PE=1 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 7.000e-19
Identity = 57/146 (39.04%), Postives = 84/146 (57.53%), Query Frame = 1 

		  

Query: 14  IKYKSAYLDIEKKGGSRTKLGLGVKQVNKFIKNVYGKPI-DEKTIPI-QAQFMLIVIQTI 73
           I Y+ +Y  +E KGG+RTKLGLG   +   +  +YGK   D+K     +A+F+LI +Q +
Sbjct: 136 IPYEKSYKGMESKGGARTKLGLGKITLKSRMGKIYGKDATDQKQYQKNEAEFLLIAVQMV 195

Query: 74  AEATRFKYIENLILADFDSFEN-RPDPKTIELEKSWQKITIGIKTSTKKGEIKPAL--DL 133
            EA+RFKYIEN + A FD     +PDPK I LEK+W  ++  I      G+    L  DL
Sbjct: 196 TEASRFKYIENKVKAKFDDANGYQPDPKAISLEKNWDSVSKVIAKVGTSGDSTVTLPGDL 255

Query: 134 TYDKDKPWIVSEVSQIAPD-MGLLKY 154
             + +KPW  + ++ +  D M LL +
Sbjct: 256 KDENNKPWTTATMNDLKNDIMALLTH 281
[Close]
BLAST of Spo20868.1 vs. ExPASy Swiss-Prot
Match: RIP2_PHYDI (Protein synthesis inhibitor PD-S2 OS=Phytolacca dioica PE=1 SV=2)

HSP 1 Score: 93.2 bits (230), Expect = 2.700e-18
Identity = 58/152 (38.16%), Postives = 90/152 (59.21%), Query Frame = 1 

		  

Query: 6   PAGANRLT--IKYKSAYLDIEKKGG-SRTKLGLGVKQVNKFIKNVYG-KPIDEKTIPIQA 65
           P  ++R++  I Y S+Y  +E K G SR+++ LG++ +N  I  + G K   +KT   +A
Sbjct: 111 PNASSRVSKNISYDSSYPALENKAGRSRSQVQLGIQILNSDIGKISGVKSFTDKT---EA 170

Query: 66  QFMLIVIQTIAEATRFKYIENLILADFDSFENRPDPKTIELEKSWQKITIGIKTSTKKGE 125
           +F+L+ IQ ++EATRFKYIEN +  +F+     P+ K + LE++W KI++ I    K G 
Sbjct: 171 EFLLVAIQMVSEATRFKYIENQVKTNFNR-AFYPNAKVLNLEETWGKISMAIH-GAKNGA 230

Query: 126 IKPALDLTYDKDKPWIVSEVSQIAPDMGLLKY 154
               L+L       WIV  V +I PD+ LLKY
Sbjct: 231 FSKPLELQNADGSKWIVLRVDEIKPDVALLKY 257
The following BLAST results are available for this feature:
BLAST of Spo20868.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902225392|gb|KNA20052.1|2.3e-5475.8hypothetical protein SOVF_0559... [more]
gi|731354828|ref|XP_010688810.1|5.2e-3050.3PREDICTED: protein synthesis i... [more]
gi|870850466|gb|KMT02566.1|5.2e-3050.3hypothetical protein BVRB_9g20... [more]
gi|99646720|emb|CAK22418.1|3.7e-2846.4ribosome-inactivating protein ... [more]
gi|731354498|ref|XP_010688629.1|6.4e-2846.4PREDICTED: antiviral protein I... [more]
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BLAST of Spo20868.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9RMG6_SPIOL1.6e-5475.8rRNA N-glycosidase OS=Spinacia... [more]
A0A0J8EHV6_BETVU3.6e-3050.3rRNA N-glycosidase OS=Beta vul... [more]
Q1H8M9_BETVU2.6e-2846.4rRNA N-glycosidase OS=Beta vul... [more]
A0A0J8BRF2_BETVU4.4e-2846.4rRNA N-glycosidase OS=Beta vul... [more]
A0A0J8BMU1_BETVU3.8e-2747.4rRNA N-glycosidase OS=Beta vul... [more]
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BLAST of Spo20868.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5
Match NameE-valueIdentityDescription
RIPL2_PHYDI4.9e-2039.4Ribosome-inactivating protein ... [more]
RIPD2_PHYDI1.4e-1940.0Dioicin-2 OS=Phytolacca dioica... [more]
RIPA_PHYAM1.4e-1940.4Antiviral protein alpha OS=Phy... [more]
RIP2_PHYAM7.0e-1939.0Antiviral protein 2 OS=Phytola... [more]
RIP2_PHYDI2.7e-1838.1Protein synthesis inhibitor PD... [more]
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BLAST of Spo20868.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 0
Match NameE-valueIdentityDescription
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001574Ribosome-inactivating proteinPFAMPF00161RIPcoord: 11..109
score: 3.3
IPR001574Ribosome-inactivating proteinunknownSSF56371Ribosome inactivating proteins (RIP)coord: 6..153
score: 6.55
IPR016138Ribosome-inactivating protein, subdomain 1GENE3D3.40.420.10coord: 6..74
score: 9.0
IPR016139Ribosome-inactivating protein, subdomain 2GENE3D4.10.470.10coord: 76..154
score: 2.4
IPR017988Ribosome-inactivating protein conserved sitePROSITEPS00275SHIGA_RICINcoord: 68..84
scor
IPR017989Ribosome-inactivating protein subgroupPRINTSPR00396SHIGARICINcoord: 64..84
score: 1.4E-12coord: 97..114
score: 1.4E-12coord: 30..44
score: 1.4

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
biological_process GO:0017148 negative regulation of translation
molecular_function GO:0030598 rRNA N-glycosylase activity
RNA-Seq Expression
   



Co-expression
Gener valueExpression
Spo216970.81Barchart | Table