Spo20995 (gene)

Overview
NameSpo20995
Typegene
OrganismSpinacia oleracea (Spinach)
DescriptionCarbohydrate-binding-like fold
Locationchr1 : 42235437 .. 42237278 (+)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAGTGATGGTGTCCCAAACTGCTTCTTTTGCCAACATTATTAACCAAAAAACATACCAATTCTTGCCAAAATCTGATAAAGTTACCACAAAATTAGCATCATATGTTAGTGTCAGCAAGATGCAGCATTATGTTAATCTTCCTCAACTAAGCTTATCCCGAACCGCGAAAGTGATCCACTGCCTCCCATTTTCACAATCTGAGGTAAGCTTTTGTCTCTATTTCACTATTTATTGCTCTTCCAACTTCCTAATTATCTATTCATTCTCTGTAACTTATTCCACTTGCAATTATGAGCAATTGGCAATTTCATGAGCTCGATCAAATGTGCGCCTTTTCGTTCTTTAATACCATGTTAATCAGTATGCTTCCCAAAATCTGAAACAGTTACAGAAATGTTGGGCATCGGCTTGACGGTTTTATCAGTTATAAAAAAGAGAGGTAAGACTAGGTATAAATGAACAGAATGTAGAAGAAAGCCTTACTTAATATAGCTATACCATTGAACAATTCTGTTTGTATACACTTTACAAACAGAGCAATATTACCTACCTACCTACCTGTTAGTAATAAGGGTAATTCATATATTCCATTGCGAAATCAGAGTTGTTATATGCTAAATGGTGTATAATGAACCAATGTGATCTTGGCTTAAGGGAAATTATAGGGATACCCAATATGCGGGTAGATTACCATTGAATAAATGCAGCGTTATCTATCCGAAGTACATCAGGTCCTAGACTAATGTGCAAATATGCATATTTTCTGGTCTTGTGAAAGAAAAGCAACCGATTTTTCAGAAATGAGCTTAATCAAAAACTTGTAAAAAATCAATTGTGTAGGTTGGGATGGAAACTGAAACACGCAGCAAGTTGGTTCGTGTGAAATTTCAGCTGGAGAAAGAGTGTCAATTTGGTGATCGTATTCTGATAGTGGGAGATGACCCAATTCTGGGAACATGGAACCTATCAGATGCCATACCCTTCATCTGGTCAGAAGGCCATATTTGGAGTCTTTCGTTGGTAAGCCATTAGGAAATGGAATTGGGTATGCTAGTTCTGGCAAATTTGCAATCAAAACTTTTATGATTGATGTTGTTTTACTCCTCCCTGCATTTGTTAGGAATTGCCTGTGGGGAAGTCTATCCAGTACAAAGCGTTGCTTAAGAACAGCATAGGTGAAATACTGTGGCAACCTGGTCCAGACAGGAGTTTCCGAGCATGGGAGACGGATAATGTTATATCTGTGATCGAAGAATGGGAAAATGCAGAAGCACAAAAGATGAGTGAAGAACAGGAAGTTGTCAAAACTAATGAGGAACAAGTGCCTAACACAGTGTTGAACAGCTATGCAGGAGATCTGGGTTCTGACAAGAATGCTAGTACTGAAAATAGAAATAGGACTCACTTAAAAAAGGAGAATCCATCTTCAAGTGGAGTCAAGGACAGAAAAGCACAGAAAAAAGAGGTAGCAATGAGAAAAGGTACCGAGAGACGGGCTAAGTCAGATGCCGAACTAGAATATGATGGAATATTGGTTACCGTTGATGAAGGTCCGGTCCTTGTGCCAGGCTTGACTCTGCTAGTATCATCTTCTTAATAAATACTCTGCTATAGAAAATTAGAAGTTGATTTGATGCACCTGGAAACAAGAATATAAAAGATTACAGATGTACCATTTTGATTTAAAAACTTGTAGCAACCAAGTTTTCCTTTGTTATAATGGAAAACAAGTCATGATTTACAACTTTTGCCATGTTACAATGCACTTCTCCAAGGATGTGTTAGATTAGTCCTACCTGCAAAATATATCCAAATACCAACAACTTTTGATTTCTCC

mRNA sequence

ATGAAAGTGATGGTGTCCCAAACTGCTTCTTTTGCCAACATTATTAACCAAAAAACATACCAATTCTTGCCAAAATCTGATAAAGTTACCACAAAATTAGCATCATATGTTAGTGTCAGCAAGATGCAGCATTATGTTAATCTTCCTCAACTAAGCTTATCCCGAACCGCGAAAGTGATCCACTGCCTCCCATTTTCACAATCTGAGGTTGGGATGGAAACTGAAACACGCAGCAAGTTGGTTCGTGTGAAATTTCAGCTGGAGAAAGAGTGTCAATTTGGTGATCGTATTCTGATAGTGGGAGATGACCCAATTCTGGGAACATGGAACCTATCAGATGCCATACCCTTCATCTGGTCAGAAGGCCATATTTGGAGTCTTTCGTTGGAATTGCCTGTGGGGAAGTCTATCCAGTACAAAGCGTTGCTTAAGAACAGCATAGGTGAAATACTGTGGCAACCTGGTCCAGACAGGAGTTTCCGAGCATGGGAGACGGATAATGTTATATCTGTGATCGAAGAATGGGAAAATGCAGAAGCACAAAAGATGAGTGAAGAACAGGAAGTTGTCAAAACTAATGAGGAACAAGTGCCTAACACAGTGTTGAACAGCTATGCAGGAGATCTGGGTTCTGACAAGAATGCTAGTACTGAAAATAGAAATAGGACTCACTTAAAAAAGGAGAATCCATCTTCAAGTGGAGTCAAGGACAGAAAAGCACAGAAAAAAGAGGTAGCAATGAGAAAAGGTACCGAGAGACGGGCTAAGTCAGATGCCGAACTAGAATATGATGGAATATTGGTTACCGTTGATGAAGGTCCGGTCCTTGTGCCAGGCTTGACTCTGCTAGTATCATCTTCTTAATAAATACTCTGCTATAGAAAATTAGAAGTTGATTTGATGCACCTGGAAACAAGAATATAAAAGATTACAGATGTACCATTTTGATTTAAAAACTTGTAGCAACCAAGTTTTCCTTTGTTATAATGGAAAACAAGTCATGATTTACAACTTTTGCCATGTTACAATGCACTTCTCCAAGGATGTGTTAGATTAGTCCTACCTGCAAAATATATCCAAATACCAACAACTTTTGATTTCTCC

Coding sequence (CDS)

ATGAAAGTGATGGTGTCCCAAACTGCTTCTTTTGCCAACATTATTAACCAAAAAACATACCAATTCTTGCCAAAATCTGATAAAGTTACCACAAAATTAGCATCATATGTTAGTGTCAGCAAGATGCAGCATTATGTTAATCTTCCTCAACTAAGCTTATCCCGAACCGCGAAAGTGATCCACTGCCTCCCATTTTCACAATCTGAGGTTGGGATGGAAACTGAAACACGCAGCAAGTTGGTTCGTGTGAAATTTCAGCTGGAGAAAGAGTGTCAATTTGGTGATCGTATTCTGATAGTGGGAGATGACCCAATTCTGGGAACATGGAACCTATCAGATGCCATACCCTTCATCTGGTCAGAAGGCCATATTTGGAGTCTTTCGTTGGAATTGCCTGTGGGGAAGTCTATCCAGTACAAAGCGTTGCTTAAGAACAGCATAGGTGAAATACTGTGGCAACCTGGTCCAGACAGGAGTTTCCGAGCATGGGAGACGGATAATGTTATATCTGTGATCGAAGAATGGGAAAATGCAGAAGCACAAAAGATGAGTGAAGAACAGGAAGTTGTCAAAACTAATGAGGAACAAGTGCCTAACACAGTGTTGAACAGCTATGCAGGAGATCTGGGTTCTGACAAGAATGCTAGTACTGAAAATAGAAATAGGACTCACTTAAAAAAGGAGAATCCATCTTCAAGTGGAGTCAAGGACAGAAAAGCACAGAAAAAAGAGGTAGCAATGAGAAAAGGTACCGAGAGACGGGCTAAGTCAGATGCCGAACTAGAATATGATGGAATATTGGTTACCGTTGATGAAGGTCCGGTCCTTGTGCCAGGCTTGACTCTGCTAGTATCATCTTCTTAA

Protein sequence

MKVMVSQTASFANIINQKTYQFLPKSDKVTTKLASYVSVSKMQHYVNLPQLSLSRTAKVIHCLPFSQSEVGMETETRSKLVRVKFQLEKECQFGDRILIVGDDPILGTWNLSDAIPFIWSEGHIWSLSLELPVGKSIQYKALLKNSIGEILWQPGPDRSFRAWETDNVISVIEEWENAEAQKMSEEQEVVKTNEEQVPNTVLNSYAGDLGSDKNASTENRNRTHLKKENPSSSGVKDRKAQKKEVAMRKGTERRAKSDAELEYDGILVTVDEGPVLVPGLTLLVSSS
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo20995.1Spo20995.1mRNA


Homology
[Close]
BLAST of Spo20995.1 vs. NCBI nr
Match: gi|902235953|gb|KNA24158.1| (hypothetical protein SOVF_018570 [Spinacia oleracea])

HSP 1 Score: 555.4 bits (1430), Expect = 5.600e-155
Identity = 286/287 (99.65%), Postives = 286/287 (99.65%), Query Frame = 1 

		  

Query: 1   MKVMVSQTASFANIINQKTYQFLPKSDKVTTKLASYVSVSKMQHYVNLPQLSLSRTAKVI 60
           MKVMVSQTASFANIINQKTYQFLPKSDK TTKLASYVSVSKMQHYVNLPQLSLSRTAKVI
Sbjct: 1   MKVMVSQTASFANIINQKTYQFLPKSDKFTTKLASYVSVSKMQHYVNLPQLSLSRTAKVI 60

Query: 61  HCLPFSQSEVGMETETRSKLVRVKFQLEKECQFGDRILIVGDDPILGTWNLSDAIPFIWS 120
           HCLPFSQSEVGMETETRSKLVRVKFQLEKECQFGDRILIVGDDPILGTWNLSDAIPFIWS
Sbjct: 61  HCLPFSQSEVGMETETRSKLVRVKFQLEKECQFGDRILIVGDDPILGTWNLSDAIPFIWS 120

Query: 121 EGHIWSLSLELPVGKSIQYKALLKNSIGEILWQPGPDRSFRAWETDNVISVIEEWENAEA 180
           EGHIWSLSLELPVGKSIQYKALLKNSIGEILWQPGPDRSFRAWETDNVISVIEEWENAEA
Sbjct: 121 EGHIWSLSLELPVGKSIQYKALLKNSIGEILWQPGPDRSFRAWETDNVISVIEEWENAEA 180

Query: 181 QKMSEEQEVVKTNEEQVPNTVLNSYAGDLGSDKNASTENRNRTHLKKENPSSSGVKDRKA 240
           QKMSEEQEVVKTNEEQVPNTVLNSYAGDLGSDKNASTENRNRTHLKKENPSSSGVKDRKA
Sbjct: 181 QKMSEEQEVVKTNEEQVPNTVLNSYAGDLGSDKNASTENRNRTHLKKENPSSSGVKDRKA 240

Query: 241 QKKEVAMRKGTERRAKSDAELEYDGILVTVDEGPVLVPGLTLLVSSS 288
           QKKEVAMRKGTERRAKSDAELEYDGILVTVDEGPVLVPGLTLLVSSS
Sbjct: 241 QKKEVAMRKGTERRAKSDAELEYDGILVTVDEGPVLVPGLTLLVSSS 287
[Close]
BLAST of Spo20995.1 vs. NCBI nr
Match: gi|1009158263|ref|XP_015897196.1| (PREDICTED: uncharacterized protein LOC107430840 isoform X1 [Ziziphus jujuba])

HSP 1 Score: 165.2 bits (417), Expect = 1.600e-37
Identity = 82/171 (47.95%), Postives = 110/171 (64.33%), Query Frame = 1 

		  

Query: 45  YVNLPQLSLSRTAKVIHCLPFSQSEVGMETETR------SKLVRVKFQLEKECQFGDRIL 104
           ++NL  +   +    I CLP +  E     +T+      SK V VKFQL+KEC FG+  L
Sbjct: 46  FLNLVLVKQRKAISPIACLPVNAQEGSEIADTQIQERYESKTVHVKFQLQKECMFGEHFL 105

Query: 105 IVGDDPILGTWNLSDAIPFIWSEGHIWSLSLELPVGKSIQYKALLKNSIGEILWQPGPDR 164
           +VGDDP+ G W+   AIP  WSEGHIW++ L++PVGKSIQ+K LLK   G I WQPGPDR
Sbjct: 106 MVGDDPMFGLWDPESAIPLDWSEGHIWTVELDIPVGKSIQFKFLLKGKTGNITWQPGPDR 165

Query: 165 SFRAWETDNVISVIEEWENAEAQKMSEEQEVVKTNEEQVPNTVLNSYAGDL 210
            F+ WET  +I+V E WENAE+QK++EE+ +   NE+   NT +   A +L
Sbjct: 166 IFQTWETIKMITVSENWENAESQKITEEERLSIQNED---NTEMMGVAENL 213
[Close]
BLAST of Spo20995.1 vs. NCBI nr
Match: gi|951037109|ref|XP_014516815.1| (PREDICTED: uncharacterized protein LOC106774358 [Vigna radiata var. radiata])

HSP 1 Score: 163.3 bits (412), Expect = 6.200e-37
Identity = 77/167 (46.11%), Postives = 113/167 (67.66%), Query Frame = 1 

		  

Query: 60  IHCLPFSQSEVGMETE----------TRSKLVRVKFQLEKECQFGDRILIVGDDPILGTW 119
           +H +P S+ +V +ET             SK VRV F+L+K+C FG++ LIVGDDP LG+W
Sbjct: 62  VHAVP-SKDQVDLETAEPEAQNNEQTNESKFVRVAFELQKDCDFGEQFLIVGDDPTLGSW 121

Query: 120 NLSDAIPFIWSEGHIWSLSLELPVGKSIQYKALLKNSIGEILWQPGPDRSFRAWETDNVI 179
           +  +A+P  WSEGH+W+   ++PVGKSIQ+K +LK   G+I+WQPGPDR    WET N I
Sbjct: 122 DPLEALPMTWSEGHVWTAEQDMPVGKSIQFKFILKGKEGDIIWQPGPDRVINTWETVNTI 181

Query: 180 SVIEEWENAEAQKMSEEQEVVKTNEEQVPNTVLNSYAGDLGSDKNAS 217
           +V E+WENAE QK++EE ++ + ++E   ++ +++ A  L  D NAS
Sbjct: 182 TVCEDWENAELQKITEEDQLAEPDKEPQIDSEVSASAEILDLDSNAS 227
[Close]
BLAST of Spo20995.1 vs. NCBI nr
Match: gi|703149528|ref|XP_010109626.1| (hypothetical protein L484_015581 [Morus notabilis])

HSP 1 Score: 159.5 bits (402), Expect = 9.000e-36
Identity = 69/120 (57.50%), Postives = 91/120 (75.83%), Query Frame = 1 

		  

Query: 77  RSKLVRVKFQLEKECQFGDRILIVGDDPILGTWNLSDAIPFIWSEGHIWSLSLELPVGKS 136
           +SK V VKF L KEC FG++ LIVGDDP+ G W+   A+P  WSEGH+W++ L++PVGKS
Sbjct: 30  QSKTVHVKFHLLKECVFGEQFLIVGDDPMFGLWDPESAVPLNWSEGHVWTVELDIPVGKS 89

Query: 137 IQYKALLKNSIGEILWQPGPDRSFRAWETDNVISVIEEWENAEAQKMSEEQEVVKTNEEQ 196
           IQ+K +LK+S GEI+WQPGP+R F+ WET N I+V E+WENAE Q +SEE++     E Q
Sbjct: 90  IQFKFILKDSTGEIVWQPGPNRVFQTWETKNTITVSEDWENAELQTVSEEEQAADKKENQ 149
[Close]
BLAST of Spo20995.1 vs. NCBI nr
Match: gi|697157891|ref|XP_009587696.1| (PREDICTED: uncharacterized protein LOC104085387 [Nicotiana tomentosiformis])

HSP 1 Score: 157.1 bits (396), Expect = 4.500e-35
Identity = 101/265 (38.11%), Postives = 141/265 (53.21%), Query Frame = 1 

		  

Query: 46  VNLPQLSLSR-TAKVIHCLPFSQSEVGMETETRSKL-----------VRVKFQLEKECQF 105
           VN+  LS+ R T + I C P  QS    +T T+S+            VRVKFQL KEC F
Sbjct: 50  VNVSSLSIQRKTVQPISCAPALQSSFDTQTHTQSEETETTDTNQSEPVRVKFQLNKECTF 109

Query: 106 GDRILIVGDDPILGTWNLSDAIPFIWSEGHIWSLSLELPVGKSIQYKALLKNSIGEILWQ 165
           G    IVGDDP+LG+WN S+A+P  WSEGH W++ L++P GK+I YK +LK     ILWQ
Sbjct: 110 GQHFHIVGDDPVLGSWNPSNAVPLDWSEGHAWTVELDIPSGKTISYKFILKGDAETILWQ 169

Query: 166 PGPDRSFRAWETDNVISVIEEWENAEAQKMSEEQEVVKTNEEQVPN-------TVLNSYA 225
            GPDR  + WET  +I+V E+W+NAE Q + EE+   + +EE   N        ++ S  
Sbjct: 170 QGPDRILQTWETKKIITVSEDWDNAELQTIVEEEPAAEQSEESAANPEILIAENLVPSTV 229

Query: 226 GDLGSDKNASTEN-------RNRTHLKKE---NPSSSGVKDRKAQKKEVAMRKGTERRAK 282
            DL  D N    N        N T +K++   + +   + + KA  K   +       +K
Sbjct: 230 VDLEDDVNEEKSNEVLAIVAENITEVKEDVNTDLNEDTIIEAKAIGKNNMVFNNEVLNSK 289
[Close]
BLAST of Spo20995.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RXI6_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_018570 PE=4 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 3.900e-155
Identity = 286/287 (99.65%), Postives = 286/287 (99.65%), Query Frame = 1 

		  

Query: 1   MKVMVSQTASFANIINQKTYQFLPKSDKVTTKLASYVSVSKMQHYVNLPQLSLSRTAKVI 60
           MKVMVSQTASFANIINQKTYQFLPKSDK TTKLASYVSVSKMQHYVNLPQLSLSRTAKVI
Sbjct: 1   MKVMVSQTASFANIINQKTYQFLPKSDKFTTKLASYVSVSKMQHYVNLPQLSLSRTAKVI 60

Query: 61  HCLPFSQSEVGMETETRSKLVRVKFQLEKECQFGDRILIVGDDPILGTWNLSDAIPFIWS 120
           HCLPFSQSEVGMETETRSKLVRVKFQLEKECQFGDRILIVGDDPILGTWNLSDAIPFIWS
Sbjct: 61  HCLPFSQSEVGMETETRSKLVRVKFQLEKECQFGDRILIVGDDPILGTWNLSDAIPFIWS 120

Query: 121 EGHIWSLSLELPVGKSIQYKALLKNSIGEILWQPGPDRSFRAWETDNVISVIEEWENAEA 180
           EGHIWSLSLELPVGKSIQYKALLKNSIGEILWQPGPDRSFRAWETDNVISVIEEWENAEA
Sbjct: 121 EGHIWSLSLELPVGKSIQYKALLKNSIGEILWQPGPDRSFRAWETDNVISVIEEWENAEA 180

Query: 181 QKMSEEQEVVKTNEEQVPNTVLNSYAGDLGSDKNASTENRNRTHLKKENPSSSGVKDRKA 240
           QKMSEEQEVVKTNEEQVPNTVLNSYAGDLGSDKNASTENRNRTHLKKENPSSSGVKDRKA
Sbjct: 181 QKMSEEQEVVKTNEEQVPNTVLNSYAGDLGSDKNASTENRNRTHLKKENPSSSGVKDRKA 240

Query: 241 QKKEVAMRKGTERRAKSDAELEYDGILVTVDEGPVLVPGLTLLVSSS 288
           QKKEVAMRKGTERRAKSDAELEYDGILVTVDEGPVLVPGLTLLVSSS
Sbjct: 241 QKKEVAMRKGTERRAKSDAELEYDGILVTVDEGPVLVPGLTLLVSSS 287
[Close]
BLAST of Spo20995.1 vs. UniProtKB/TrEMBL
Match: W9S2V9_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_015581 PE=4 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 6.300e-36
Identity = 69/120 (57.50%), Postives = 91/120 (75.83%), Query Frame = 1 

		  

Query: 77  RSKLVRVKFQLEKECQFGDRILIVGDDPILGTWNLSDAIPFIWSEGHIWSLSLELPVGKS 136
           +SK V VKF L KEC FG++ LIVGDDP+ G W+   A+P  WSEGH+W++ L++PVGKS
Sbjct: 30  QSKTVHVKFHLLKECVFGEQFLIVGDDPMFGLWDPESAVPLNWSEGHVWTVELDIPVGKS 89

Query: 137 IQYKALLKNSIGEILWQPGPDRSFRAWETDNVISVIEEWENAEAQKMSEEQEVVKTNEEQ 196
           IQ+K +LK+S GEI+WQPGP+R F+ WET N I+V E+WENAE Q +SEE++     E Q
Sbjct: 90  IQFKFILKDSTGEIVWQPGPNRVFQTWETKNTITVSEDWENAELQTVSEEEQAADKKENQ 149
[Close]
BLAST of Spo20995.1 vs. UniProtKB/TrEMBL
Match: A0A061EQF6_THECC (Carbohydrate-binding-like fold, putative isoform 1 OS=Theobroma cacao GN=TCM_021717 PE=4 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 5.300e-35
Identity = 90/220 (40.91%), Postives = 124/220 (56.36%), Query Frame = 1 

		  

Query: 15  INQKTYQFLPKSDKVTTKLASYVSVSKMQHYVNLPQLSLSRTAKVIHCLPFSQSEVGMET 74
           +N+      P    V  +L   +SV   +    L   SLS  ++V     F  +E     
Sbjct: 29  LNRGEVCLFPSKKLVRIRLLRLLSVQHRRLQPVLSSSSLSPDSQV----DFETAETQPAE 88

Query: 75  ETRSKLVRVKFQLEKECQFGDRILIVGDDPILGTWNLSDAIPFIWSEGHIWSLSLELPVG 134
           E  SK V VKFQL+KEC FG+   IVGD P+LG W+   AIP  W +GH+W++ L++PVG
Sbjct: 89  ENPSKTVHVKFQLQKECSFGEHFFIVGDHPMLGIWDPESAIPLNWLKGHVWTVELDIPVG 148

Query: 135 KSIQYKALLKNSIGEILWQPGPDRSFRAWETDNVISVIEEWENAEAQKMSEEQE------ 194
           KSIQ+K +LK S G +LWQPGPDR F++WET+N I V E+WE AE QK+ EE+       
Sbjct: 149 KSIQFKFVLKTSTGNLLWQPGPDRIFKSWETENTIIVSEDWEEAEYQKLIEEEPSANQDG 208

Query: 195 VVKTNEEQVPNTVLNSYAGDLGSDKNASTENRNRTHLKKE 229
            V  +E  +    L     +L SD    +E  + T+L+KE
Sbjct: 209 PVLDSEMAIVAENLTPPKEELVSDMELVSETDSITNLEKE 244
[Close]
BLAST of Spo20995.1 vs. UniProtKB/TrEMBL
Match: A0A0R0L9H1_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_01G115000 PE=4 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 9.100e-35
Identity = 67/123 (54.47%), Postives = 89/123 (72.36%), Query Frame = 1 

		  

Query: 73  ETETRSKLVRVKFQLEKECQFGDRILIVGDDPILGTWNLSDAIPFIWSEGHIWSLSLELP 132
           E    SK VRV FQLEK C FG++ LIVGDDP+LG+W+  +A+P  W EGH+W++ L++P
Sbjct: 85  EQTNESKFVRVSFQLEKNCNFGEQFLIVGDDPVLGSWDPLEALPMTWFEGHVWAVELDMP 144

Query: 133 VGKSIQYKALLKNSIGEILWQPGPDRSFRAWETDNVISVIEEWENAEAQKMSEEQEVVKT 192
            GKS Q+K +LK   G+I+WQPG DR    WET N I V+E+WENAE QK+ EE ++ + 
Sbjct: 145 AGKSFQFKFILKGKGGDIIWQPGLDRMIHTWETMNRIIVLEDWENAELQKIIEEDQLAEP 204

Query: 193 NEE 196
           NEE
Sbjct: 205 NEE 207
[Close]
BLAST of Spo20995.1 vs. UniProtKB/TrEMBL
Match: A0A0B2SVU5_GLYSO (Alpha-amylase OS=Glycine soja GN=glysoja_045299 PE=4 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 9.100e-35
Identity = 67/123 (54.47%), Postives = 89/123 (72.36%), Query Frame = 1 

		  

Query: 73  ETETRSKLVRVKFQLEKECQFGDRILIVGDDPILGTWNLSDAIPFIWSEGHIWSLSLELP 132
           E    SK VRV FQLEK C FG++ LIVGDDP+LG+W+  +A+P  W EGH+W++ L++P
Sbjct: 85  EQTNESKFVRVSFQLEKNCNFGEQFLIVGDDPVLGSWDPLEALPMTWFEGHVWAVELDMP 144

Query: 133 VGKSIQYKALLKNSIGEILWQPGPDRSFRAWETDNVISVIEEWENAEAQKMSEEQEVVKT 192
            GKS Q+K +LK   G+I+WQPG DR    WET N I V+E+WENAE QK+ EE ++ + 
Sbjct: 145 AGKSFQFKFILKGKGGDIIWQPGLDRMIHTWETMNRIIVLEDWENAELQKIIEEDQLAEP 204

Query: 193 NEE 196
           NEE
Sbjct: 205 NEE 207
[Close]
BLAST of Spo20995.1 vs. ExPASy Swiss-Prot
Match: AMYG_ARTBC (Glucoamylase ARB_02327-1 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) GN=ARB_02327-1 PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 5.500e-9
Identity = 30/95 (31.58%), Postives = 54/95 (56.84%), Query Frame = 1 

		  

Query: 83  VKFQLEKECQFGDRILIVGDDPILGTWNLSDAIPF---IWSEG-HIWSLSLELPVGKSIQ 142
           V+F+L    Q G+ + +VG  P LG+W++  A+P    I+++  H W + +ELP   + +
Sbjct: 512 VRFRLLATTQVGEDVFLVGSIPELGSWDVKKAVPLNADIYADNCHQWYVDIELPTAVAFE 571

Query: 143 YKALLKNSIGEILWQPGPDRSFRAWETDNVISVIE 174
           YK + K   GE++W+  P+R +   +T  V   I+
Sbjct: 572 YKFIRKRG-GEVVWEQDPNRKYTVPQTCGVSGAIK 605
[Close]
BLAST of Spo20995.1 vs. ExPASy Swiss-Prot
Match: PWD_ARATH (Phosphoglucan, water dikinase, chloroplastic OS=Arabidopsis thaliana GN=GWD3 PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 2.300e-7
Identity = 48/181 (26.52%), Postives = 83/181 (45.86%), Query Frame = 1 

		  

Query: 44  HYVNLPQLS--LSRTAKVIHCLPFSQSEVGMETETRS---KLVRVKFQLEKECQFGDRIL 103
           H VNL   S  L  +   + C   S S +  + + +      VR+  +L+ +  FGD + 
Sbjct: 32  HRVNLSHQSHRLRNSNSRLTCTATSSSTIEEQRKKKDGSGTKVRLNVRLDHQVNFGDHVA 91

Query: 104 IVGDDPILGTWNLSDAIPFIWSEGHIWSLSLELPVGKSIQYKALLKNSIGEILWQPGPDR 163
           + G    +G+W      P  WSE   W   LEL  G+ ++YK ++  + G + W+ G +R
Sbjct: 92  MFGSAKEIGSWKKKS--PLNWSENG-WVCELELDGGQVLEYKFVIVKNDGSLSWESGDNR 151

Query: 164 SFRAWETDNVISVIEEWENAEAQKMSEEQEVVKTNEEQVPNTVLNSYAGDLGSDKNASTE 220
             +   + N  SV+  W+ A  + +   QEV   N++ V +        D+G D+   +E
Sbjct: 152 VLKVPNSGN-FSVVCHWD-ATRETLDLPQEV--GNDDDVGDGGHERDNHDVGDDRVVGSE 205
[Close]
BLAST of Spo20995.1 vs. ExPASy Swiss-Prot
Match: AMYG_NEUCR (Glucoamylase OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) GN=gla-1 PE=1 SV=3)

HSP 1 Score: 57.0 bits (136), Expect = 3.900e-7
Identity = 39/142 (27.46%), Postives = 61/142 (42.96%), Query Frame = 1 

		  

Query: 23  LPKSDKVTTKLASYVSVSKMQHYVNLPQLSLSRTAKVIHCLPFSQSEVGMETETRSKLVR 82
           LP S   +T   SY + +      NL   S + T       P +Q+    + E     V 
Sbjct: 480 LPGSCSASTVAGSYATATATSFPANLTPASTTVT-------PPTQTGCAADHE-----VL 539

Query: 83  VKFQLEKECQFGDRILIVGDDPILGTWNLSDAIPF----IWSEGHIWSLSLELPVGKSIQ 142
           V F  +    +G  + +VG    LG W  +  +        S   +WS ++ LP G S +
Sbjct: 540 VTFNEKVTTSYGQTVKVVGSIAALGNWAPASGVTLSAKQYSSSNPLWSTTIALPQGTSFK 599

Query: 143 YKALLKNSIGEILWQPGPDRSF 161
           YK ++ NS G + W+  PDRS+
Sbjct: 600 YKYVVVNSDGSVKWENDPDRSY 609
[Close]
BLAST of Spo20995.1 vs. ExPASy Swiss-Prot
Match: AMYG_ASPKA (Glucoamylase I OS=Aspergillus kawachii GN=gaI PE=1 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 1.500e-6
Identity = 29/106 (27.36%), Postives = 48/106 (45.28%), Query Frame = 1 

		  

Query: 76  TRSKLVRVKFQLEKECQFGDRILIVGDDPILGTWNLSDAIPF----IWSEGHIWSLSLEL 135
           T    V V F L     +G+ I +VG    LG W  SD I        S   +W +++ L
Sbjct: 533 TTPTAVAVTFDLTATTTYGENIYLVGSISQLGDWETSDGIALSADKYTSSNPLWYVTVTL 592

Query: 136 PVGKSIQYKALLKNSIGEILWQPGPDRSFRAWET--DNVISVIEEW 176
           P G+S +YK +   S   + W+  P+R +   +   ++  +V + W
Sbjct: 593 PAGESFEYKFIRVESDDSVEWESDPNREYTVPQACGESTATVTDTW 638
[Close]
BLAST of Spo20995.1 vs. ExPASy Swiss-Prot
Match: AMYG_ASPAW (Glucoamylase OS=Aspergillus awamori GN=GLAA PE=1 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 2.000e-6
Identity = 30/113 (26.55%), Postives = 50/113 (44.25%), Query Frame = 1 

		  

Query: 52  SLSRTAKVIHCLPFSQSEVGMETETRSKLVRVKFQLEKECQFGDRILIVGDDPILGTWNL 111
           S++ T+K       + +     + T    V V F L     +G+ I +VG    LG W  
Sbjct: 510 SVTSTSKTTATASKTSTSTSSTSCTTPTAVAVTFDLTATTTYGENIYLVGSISQLGDWET 569

Query: 112 SDAIPF----IWSEGHIWSLSLELPVGKSIQYKALLKNSIGEILWQPGPDRSF 161
           SD I        S   +W +++ LP G+S +YK +   S   + W+  P+R +
Sbjct: 570 SDGIALSADKYTSSDPLWYVTVTLPAGESFEYKFIRIESDDSVEWESDPNREY 622
[Close]
BLAST of Spo20995.1 vs. TAIR (Arabidopsis)
Match: AT5G01260.2 (Carbohydrate-binding-like fold)

HSP 1 Score: 149.1 bits (375), Expect = 4.300e-36
Identity = 87/252 (34.52%), Postives = 136/252 (53.97%), Query Frame = 1 

		  

Query: 41  KMQHYVNLPQLSLSRTAKVIHCLPFSQSEV--------GMETETRSKLVRVKFQLEKECQ 100
           K  H++   +L  +++++++  +P   S +          E E  +K VRV+FQL KEC 
Sbjct: 39  KTFHHIKFLRLDSAQSSRILKPVPLRSSSIKDSQVNVEDEEIEASNKTVRVRFQLRKECV 98

Query: 101 FGDRILIVGDDPILG-TWNLSDAIPFIWSEGHIWSLSLELPVGKSIQYKALLKNSIGEIL 160
           FG+   IVGDDP+ G  W+   A+P  WS+G++W++ L+LPVG+ +++K LLK   GEIL
Sbjct: 99  FGEHFFIVGDDPVFGGLWDPETALPLNWSDGNVWTVDLDLPVGRLVEFKLLLKAQTGEIL 158

Query: 161 WQPGPDRSFRAWETDNVISVIEEWENAEAQKMSEEQEVVKTNEEQVPNTVLNSYAGDLGS 220
           WQPGP+R+   WET+  I + E+W+NA+ Q M EE  V  TN   +         G    
Sbjct: 159 WQPGPNRALETWETNKTIRICEDWDNADLQMMIEEDFVPYTNISSI---------GSEDE 218

Query: 221 DKNASTENRNRTHLKKENPSSSGVKDRKAQKKEVAMRKGTERRAKSDAELEYDGI---LV 280
           D+   +  +N + +  EN  +  V D  AQ    +++        + A    + I   + 
Sbjct: 219 DEVLGSVQQNSSVVAVEN--AGYVSDESAQNSSFSIQSEKTMEPSNGALTAREVIKEAMF 278
[Close]
BLAST of Spo20995.1 vs. TAIR (Arabidopsis)
Match: AT5G26570.1 (catalytics;carbohydrate kinases;phosphoglucan, water dikinases)

HSP 1 Score: 57.8 bits (138), Expect = 1.300e-8
Identity = 48/181 (26.52%), Postives = 83/181 (45.86%), Query Frame = 1 

		  

Query: 44  HYVNLPQLS--LSRTAKVIHCLPFSQSEVGMETETRS---KLVRVKFQLEKECQFGDRIL 103
           H VNL   S  L  +   + C   S S +  + + +      VR+  +L+ +  FGD + 
Sbjct: 32  HRVNLSHQSHRLRNSNSRLTCTATSSSTIEEQRKKKDGSGTKVRLNVRLDHQVNFGDHVA 91

Query: 104 IVGDDPILGTWNLSDAIPFIWSEGHIWSLSLELPVGKSIQYKALLKNSIGEILWQPGPDR 163
           + G    +G+W      P  WSE   W   LEL  G+ ++YK ++  + G + W+ G +R
Sbjct: 92  MFGSAKEIGSWKKKS--PLNWSENG-WVCELELDGGQVLEYKFVIVKNDGSLSWESGDNR 151

Query: 164 SFRAWETDNVISVIEEWENAEAQKMSEEQEVVKTNEEQVPNTVLNSYAGDLGSDKNASTE 220
             +   + N  SV+  W+ A  + +   QEV   N++ V +        D+G D+   +E
Sbjct: 152 VLKVPNSGN-FSVVCHWD-ATRETLDLPQEV--GNDDDVGDGGHERDNHDVGDDRVVGSE 205
The following BLAST results are available for this feature:
BLAST of Spo20995.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902235953|gb|KNA24158.1|5.6e-15599.6hypothetical protein SOVF_0185... [more]
gi|1009158263|ref|XP_015897196.1|1.6e-3747.9PREDICTED: uncharacterized pro... [more]
gi|951037109|ref|XP_014516815.1|6.2e-3746.1PREDICTED: uncharacterized pro... [more]
gi|703149528|ref|XP_010109626.1|9.0e-3657.5hypothetical protein L484_0155... [more]
gi|697157891|ref|XP_009587696.1|4.5e-3538.1PREDICTED: uncharacterized pro... [more]
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BLAST of Spo20995.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9RXI6_SPIOL3.9e-15599.6Uncharacterized protein OS=Spi... [more]
W9S2V9_9ROSA6.3e-3657.5Uncharacterized protein OS=Mor... [more]
A0A061EQF6_THECC5.3e-3540.9Carbohydrate-binding-like fold... [more]
A0A0R0L9H1_SOYBN9.1e-3554.4Uncharacterized protein OS=Gly... [more]
A0A0B2SVU5_GLYSO9.1e-3554.4Alpha-amylase OS=Glycine soja ... [more]
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BLAST of Spo20995.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5
Match NameE-valueIdentityDescription
AMYG_ARTBC5.5e-931.5Glucoamylase ARB_02327-1 OS=Ar... [more]
PWD_ARATH2.3e-726.5Phosphoglucan, water dikinase,... [more]
AMYG_NEUCR3.9e-727.4Glucoamylase OS=Neurospora cra... [more]
AMYG_ASPKA1.5e-627.3Glucoamylase I OS=Aspergillus ... [more]
AMYG_ASPAW2.0e-626.5Glucoamylase OS=Aspergillus aw... [more]
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BLAST of Spo20995.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 2
Match NameE-valueIdentityDescription
AT5G01260.24.3e-3634.5Carbohydrate-binding-like fold[more]
AT5G26570.11.3e-826.5catalytics;carbohydrate kinase... [more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002044Carbohydrate binding module family 20PFAMPF00686CBM_20coord: 81..168
score: 2.8
IPR002044Carbohydrate binding module family 20SMARTSM01065CBM_20_2coord: 80..172
score: 4.6
IPR002044Carbohydrate binding module family 20PROFILEPS51166CBM20coord: 75..177
score: 1
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10coord: 78..173
score: 1.4
IPR013784Carbohydrate-binding-like foldunknownSSF49452Starch-binding domain-likecoord: 78..173
score: 1.15
IPR015902Glycoside hydrolase, family 13PANTHERPTHR10357ALPHA-AMYLASEcoord: 16..199
score: 1.7
NoneNo IPR availableunknownCoilCoilcoord: 175..195
scor
NoneNo IPR availablePANTHERPTHR10357:SF149SUBFAMILY NOT NAMEDcoord: 16..199
score: 1.7

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
biological_process GO:0007049 cell cycle
biological_process GO:0006265 DNA topological change
biological_process GO:1901137 carbohydrate derivative biosynthetic process
biological_process GO:0019252 starch biosynthetic process
biological_process GO:0044237 cellular metabolic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0048868 pollen tube development
biological_process GO:0015721 bile acid and bile salt transport
biological_process GO:0035725 sodium ion transmembrane transport
biological_process GO:0051301 cell division
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0010020 chloroplast fission
biological_process GO:0009902 chloroplast relocation
biological_process GO:0007017 microtubule-based process
biological_process GO:0051258 protein polymerization
biological_process GO:0009220 pyrimidine ribonucleotide biosynthetic process
biological_process GO:0009637 response to blue light
biological_process GO:0051252 regulation of RNA metabolic process
biological_process GO:0006396 RNA processing
biological_process GO:0030488 tRNA methylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0009106 lipoate metabolic process
biological_process GO:0045893 positive regulation of transcription, DNA-templated
biological_process GO:0009695 jasmonic acid biosynthetic process
biological_process GO:0019288 isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
biological_process GO:0016226 iron-sulfur cluster assembly
biological_process GO:0009117 nucleotide metabolic process
biological_process GO:0006733 oxidoreduction coenzyme metabolic process
biological_process GO:0016310 phosphorylation
biological_process GO:0006546 glycine catabolic process
biological_process GO:0019344 cysteine biosynthetic process
biological_process GO:0009108 coenzyme biosynthetic process
biological_process GO:0010207 photosystem II assembly
biological_process GO:0015995 chlorophyll biosynthetic process
biological_process GO:0009965 leaf morphogenesis
biological_process GO:0042545 cell wall modification
biological_process GO:0019748 secondary metabolic process
biological_process GO:0030154 cell differentiation
biological_process GO:0009073 aromatic amino acid family biosynthetic process
biological_process GO:0006636 unsaturated fatty acid biosynthetic process
biological_process GO:0006766 vitamin metabolic process
cellular_component GO:0009570 chloroplast stroma
cellular_component GO:0009535 chloroplast thylakoid membrane
cellular_component GO:0005874 microtubule
cellular_component GO:0005737 cytoplasm
cellular_component GO:0009507 chloroplast
cellular_component GO:0005887 integral component of plasma membrane
cellular_component GO:0009941 chloroplast envelope
cellular_component GO:0009536 plastid
cellular_component GO:0009501 amyloplast
cellular_component GO:0005694 chromosome
molecular_function GO:0042802 identical protein binding
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0051903 S-(hydroxymethyl)glutathione dehydrogenase activity
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0009020 tRNA (guanosine-2'-O-)-methyltransferase activity
molecular_function GO:2001070 starch binding
molecular_function GO:0003723 RNA binding
molecular_function GO:0004540 ribonuclease activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0004518 nuclease activity
molecular_function GO:0043621 protein self-association
molecular_function GO:0003924 GTPase activity
molecular_function GO:0005525 GTP binding
molecular_function GO:0016301 kinase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0003917 DNA topoisomerase type I activity
molecular_function GO:0030246 carbohydrate binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0009011 starch synthase activity
molecular_function GO:0004022 alcohol dehydrogenase (NAD) activity
molecular_function GO:0050662 coenzyme binding
molecular_function GO:0008508 bile acid:sodium symporter activity
molecular_function GO:0005515 protein binding
RNA-Seq Expression