Spo21095 (gene)

Overview
NameSpo21095
Typegene
OrganismSpinacia oleracea (Spinach)
DescriptionMitotic checkpoint serine/threonine-protein kinase BUB1, putative isoform 1
LocationSpoScf_01775 : 93646 .. 96616 (-)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATCGATGAGTTCGCCAAGGGAGATATCATCCCAATCATGTGGCCAGCCATCATCTTAAAGCTCTCAACTAGTGATCGATCGGGGATCGTTTGGGCTAATTCTCAATGGTTCACCTCCCCCTCCCTTTTACCCCTTTTTTCTAATTTGATATTATTGAATCAATAAAGGTGAATCTGCTTATGGACGTTCTTAGCTTTGGTGTTACTAAACAAATAAAGGTGAATCTCCAACTGAGTGAAGATGTGAAGATGGTGAGAAGGGGGAAAATTGCCCACTATCGAGAGAGGTTGGATGAGACATTAGCATCTGAAAATATCACTGATGCGCAGAAACTAAAGAATCTTGTTAAAGATCATATCTTACGATCATCGGACCATAATCCCATAGGTTGGCTTTCTTGAAGTTACTTGCTAGTTTGCCTAATTTGATATTATTGAATAAATAAAGGTGAATCTCCATCTAATATTCAAGTTTAATTATTTTTTAGGTCTTAAATCTGCTTTAAAAGCTAATGGATTATTGAAAGTATAGTATAAGTTAAACCGTAAAGTAATAGTGATTCCAAAACAAAAACATACTTTAAGGTAGTAAGTTACAATAAAATACAAAAGTGGTGTATTTGTAAGGTAATAAGTAATAGTAATAGTAATAGTAATAGTAATAGTACTAGTAGTAGTAATAGTACTAGTAGTAGTAATAACAATAGTAGTAATAATAATAATAATAATAATAACAAAACAAAAAAAAAACTCATAATAATAATATCACTTACTTGTTAATATCATCAGAAGAATAGCCAGTTACACCACCAAATACAACTAATACATAATCCACATCAAGTGATTGCATAATTTCGTATGCTTCGTCTTCATAAGACGACATTGCCCTTCCAACTGTAGCAATGTGGGTGTTATTCCAAGTGTTGTTATCAACAATGACTGTCCTGTTTCCCATTGCAGTAATCTGATATCCATAATCCCACCATGACATCACTTTTGCATCAGCAGGAGTGTTTTGCCGAAGCCAGTAATATGCCTCACGGTAATCATCAAAGATAACCCGGTTCCCATGTGCACCCCGTGCAGCTAAAACTATTGACGGAGATGAATAAGCCTCTGATGTAACCCACGTACAGTGGATAACATATCTGCTGAGCAAGTAAAATGCACCCAGAAGCAACCCAATAGCAACATTTCTTTGGAAAGGCAAGGACTGATCAACAGAAGTCTGCTTCATCAAACCAAAGAGCAAGAAGAAAAGAAAAGATGAATGAGAAATTCATGCTCAGAGACATGCATATCATCACTAACTAATTAAAGCATAACATAAGCTATTAACCACCCTTCTAAACTGCCAAAGGTGAGGTTAATGCCAGACCACTGTACCAGAAGTATAGCAGAGCTTATGAACAAAGATTAATCTAGAAACATTGACCCGATCCGCAAACCCAACCAAGCTCACAGGGGAAAATAGGTTCTTGAACATAAGTTCTAGACCCAGGACCCAGAAAATACCAGGTAAAAACTAAAAATAACTGCATAATGTAGCCAGAAACTAACCTAATTCTAGCCTCAAATACCCAATCTTAAAATGATATGATTAACCAGTATGGCAAGTTATGATACATCAGCAAACAATCACATTATGTCAGTAAATCCTATGTTACTTGGACTCTTCGTTTTACCTCAAGTACCCGTGTCGGATCCTCGACACTTGGACATGGGTATGGACACTGGACACTTCATTTTGGTCTAAAATTAAGGAAATTTTCCACAATTTAGCCGTGTCGGACACTTGGACACGTACCCGTATCCGACACTCGTACCCGAGTCTGAGTAACATAGAGTAAATCAACTGGTACAATACAGAAACAATTCAATTAAATGAAAATAAAAAAATCCATATCAATCAACCAGACACCAAGCAAAAGTTCATGTAAAAACCGAAGAAATAATACATGACATTAAGTTTAAGACATGTATTAAAAATTGAAACGCAAGCAAAATCCGAAGTACATAAAACAAGAATCAGGTACACATCATACCATGGTTGAAAAAGCTCTCCTTTTCTTCTTTCTTTTAGTTGTCTTTTTCGTGCAACCCAAATTTCAACATGGATATCATTTAATTTAGAGATTATACAACATCGAAAAATCTTGCAACTTAAGCGAGTGTTTTATGCAAATTTCTCAAAGGAGAAATAGCACAAGCAAAAAGCGGTAAATTAGTCTTCCTTCATCAATCAGCAGTTGCCACTAATTATTCTCAGATATCTGTTGTTGATGACACTTATGTTTTGCATCAATAAGCATATTGATGAAGTTTAATCGAACTCCTATGTTATCAATACCAAGAAAAGGAAGAAAATATATAGACATACAATAGGTGACTAACATTTAAATAGGTTGTCCCTCGGTGTGAATATAGCATAAGTTGATCTACTTGCAGCCATATACGAAAAACTACAAGAACCTAAAATTGATCCCAGCTCCGGAAGCTCTTTGGAAGTTCAAGCGTCTAAGTTAAACTATGTTTGAAAAGAACAGACAACTATGCTTCTATTATTACAGTAATAAATCAGACATGGAAGGAAAAAGAGCAAACAACCCCCTGTAAACCTAGACAAGGAGTATAACATGAATCTAGTAGCTGAACATACCTTAGTAGAAGCCTTGACACTAGCACTTCCTTTAACGGAGCCAGAATGAACCTTGCTCTTAGACCTCAACAGTGAAGTTAAATTCTTTATTGTTGCAGAAACTGCAATACCACTTATAAGGCATACAGCAGGAGTGGCAACTAAGATCAAACGAACCATAACACCAGCGAAATACATGCTGGTCAAGCCGTACATAACAATAAATATGGTGGCATCTGTCAACCTCTTGAAGCAAAAATATAGACCTGCCGGGAAAAGGAAAAGCAGAATATGGAAATCAAACATAAATGAAGACCAAGCTGTAGGTTGA

mRNA sequence

ATGATCGATGAGTTCGCCAAGGGAGATATCATCCCAATCATGTGGCCAGCCATCATCTTAAAGCTCTCAACTAGTGATCGATCGGGGATCGTGAATCTGCTTATGGACGTTCTTAGCTTTGGTGTTACTAAACAAATAAAGGTGAATCTCCAACTGAGTGAAGATGTGAAGATGGTGAGAAGGGGGAAAATTGCCCACTATCGAGAGAGGTTGGATGAGACATTAGCATCTGAAAATATCACTGATGCGCAGAAACTAAAGAATCTTGTTAAAGATCATATCTTACGATCATCGGACCATAATCCCATAGAAACTGCAATACCACTTATAAGGCATACAGCAGGAGTGGCAACTAAGATCAAACGAACCATAACACCAGCGAAATACATGCTGGTCAAGCCGTACATAACAATAAATATGGTGGCATCTGTCAACCTCTTGAAGCAAAAATATAGACCTGCCGGGAAAAGGAAAAGCAGAATATGGAAATCAAACATAAATGAAGACCAAGCTGTAGGTTGA

Coding sequence (CDS)

ATGATCGATGAGTTCGCCAAGGGAGATATCATCCCAATCATGTGGCCAGCCATCATCTTAAAGCTCTCAACTAGTGATCGATCGGGGATCGTGAATCTGCTTATGGACGTTCTTAGCTTTGGTGTTACTAAACAAATAAAGGTGAATCTCCAACTGAGTGAAGATGTGAAGATGGTGAGAAGGGGGAAAATTGCCCACTATCGAGAGAGGTTGGATGAGACATTAGCATCTGAAAATATCACTGATGCGCAGAAACTAAAGAATCTTGTTAAAGATCATATCTTACGATCATCGGACCATAATCCCATAGAAACTGCAATACCACTTATAAGGCATACAGCAGGAGTGGCAACTAAGATCAAACGAACCATAACACCAGCGAAATACATGCTGGTCAAGCCGTACATAACAATAAATATGGTGGCATCTGTCAACCTCTTGAAGCAAAAATATAGACCTGCCGGGAAAAGGAAAAGCAGAATATGGAAATCAAACATAAATGAAGACCAAGCTGTAGGTTGA

Protein sequence

MIDEFAKGDIIPIMWPAIILKLSTSDRSGIVNLLMDVLSFGVTKQIKVNLQLSEDVKMVRRGKIAHYRERLDETLASENITDAQKLKNLVKDHILRSSDHNPIETAIPLIRHTAGVATKIKRTITPAKYMLVKPYITINMVASVNLLKQKYRPAGKRKSRIWKSNINEDQAVG
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo21095.1Spo21095.1mRNA


Homology
[Close]
BLAST of Spo21095.1 vs. NCBI nr
Match: gi|902234611|gb|KNA23511.1| (hypothetical protein SOVF_024190 [Spinacia oleracea])

HSP 1 Score: 82.8 bits (203), Expect = 6.500e-13
Identity = 39/47 (82.98%), Postives = 45/47 (95.74%), Query Frame = 1 

		  

Query: 58  MVRRGKIAHYRERLDETLASENITDAQKLKNLVKDHILRSSDHNPIE 105
           MV++GKIAHYRERLDETLASEN+TD +KLKNLVKD ILRSSD+NPI+
Sbjct: 1   MVKKGKIAHYRERLDETLASENLTDEEKLKNLVKDQILRSSDNNPID 47
[Close]
BLAST of Spo21095.1 vs. NCBI nr
Match: gi|731333135|ref|XP_010677572.1| (PREDICTED: uncharacterized protein LOC104893183 isoform X2 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 61.2 bits (147), Expect = 2.000e-6
Identity = 31/44 (70.45%), Postives = 36/44 (81.82%), Query Frame = 1 

		  

Query: 58  MVRRGKIAHYRERLDETLASENITDAQKLKNLVKDHILRSSDHN 102
           M ++GKIA YRERLDETLAS+++TD   LK LVKDHILRSS  N
Sbjct: 1   MAKKGKIALYRERLDETLASDDLTDENNLKILVKDHILRSSLDN 44
[Close]
BLAST of Spo21095.1 vs. NCBI nr
Match: gi|870860209|gb|KMT11572.1| (hypothetical protein BVRB_5g108860 isoform C [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 61.2 bits (147), Expect = 2.000e-6
Identity = 31/44 (70.45%), Postives = 36/44 (81.82%), Query Frame = 1 

		  

Query: 58  MVRRGKIAHYRERLDETLASENITDAQKLKNLVKDHILRSSDHN 102
           M ++GKIA YRERLDETLAS+++TD   LK LVKDHILRSS  N
Sbjct: 1   MAKKGKIALYRERLDETLASDDLTDENNLKILVKDHILRSSLDN 44
[Close]
BLAST of Spo21095.1 vs. NCBI nr
Match: gi|731333129|ref|XP_010677568.1| (PREDICTED: uncharacterized protein LOC104893183 isoform X1 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 61.2 bits (147), Expect = 2.000e-6
Identity = 31/44 (70.45%), Postives = 36/44 (81.82%), Query Frame = 1 

		  

Query: 58  MVRRGKIAHYRERLDETLASENITDAQKLKNLVKDHILRSSDHN 102
           M ++GKIA YRERLDETLAS+++TD   LK LVKDHILRSS  N
Sbjct: 1   MAKKGKIALYRERLDETLASDDLTDENNLKILVKDHILRSSLDN 44
[Close]
BLAST of Spo21095.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RVF3_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_024190 PE=4 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 4.500e-13
Identity = 39/47 (82.98%), Postives = 45/47 (95.74%), Query Frame = 1 

		  

Query: 58  MVRRGKIAHYRERLDETLASENITDAQKLKNLVKDHILRSSDHNPIE 105
           MV++GKIAHYRERLDETLASEN+TD +KLKNLVKD ILRSSD+NPI+
Sbjct: 1   MVKKGKIAHYRERLDETLASENLTDEEKLKNLVKDQILRSSDNNPID 47
[Close]
BLAST of Spo21095.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CDB1_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_5g108860 PE=4 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 1.400e-6
Identity = 31/44 (70.45%), Postives = 36/44 (81.82%), Query Frame = 1 

		  

Query: 58  MVRRGKIAHYRERLDETLASENITDAQKLKNLVKDHILRSSDHN 102
           M ++GKIA YRERLDETLAS+++TD   LK LVKDHILRSS  N
Sbjct: 1   MAKKGKIALYRERLDETLASDDLTDENNLKILVKDHILRSSLDN 44
[Close]
BLAST of Spo21095.1 vs. UniProtKB/TrEMBL
Match: A0A0J8F6I8_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_5g108860 PE=4 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 1.400e-6
Identity = 31/44 (70.45%), Postives = 36/44 (81.82%), Query Frame = 1 

		  

Query: 58  MVRRGKIAHYRERLDETLASENITDAQKLKNLVKDHILRSSDHN 102
           M ++GKIA YRERLDETLAS+++TD   LK LVKDHILRSS  N
Sbjct: 1   MAKKGKIALYRERLDETLASDDLTDENNLKILVKDHILRSSLDN 44
The following BLAST results are available for this feature:
BLAST of Spo21095.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 4
Match NameE-valueIdentityDescription
gi|902234611|gb|KNA23511.1|6.5e-1382.9hypothetical protein SOVF_0241... [more]
gi|731333135|ref|XP_010677572.1|2.0e-670.4PREDICTED: uncharacterized pro... [more]
gi|870860209|gb|KMT11572.1|2.0e-670.4hypothetical protein BVRB_5g10... [more]
gi|731333129|ref|XP_010677568.1|2.0e-670.4PREDICTED: uncharacterized pro... [more]
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BLAST of Spo21095.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 3
Match NameE-valueIdentityDescription
A0A0K9RVF3_SPIOL4.5e-1382.9Uncharacterized protein OS=Spi... [more]
A0A0J8CDB1_BETVU1.4e-670.4Uncharacterized protein OS=Bet... [more]
A0A0J8F6I8_BETVU1.4e-670.4Uncharacterized protein OS=Bet... [more]
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BLAST of Spo21095.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Spo21095.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 0
Match NameE-valueIdentityDescription
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GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006888 ER to Golgi vesicle-mediated transport
biological_process GO:0006554 lysine catabolic process
biological_process GO:0006897 endocytosis
biological_process GO:0006886 intracellular protein transport
biological_process GO:0006568 tryptophan metabolic process
biological_process GO:0006099 tricarboxylic acid cycle
biological_process GO:0006464 cellular protein modification process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009506 plasmodesma
cellular_component GO:0045252 oxoglutarate dehydrogenase complex
cellular_component GO:0005774 vacuolar membrane
cellular_component GO:0030127 COPII vesicle coat
cellular_component GO:0030132 clathrin coat of coated pit
cellular_component GO:0030130 clathrin coat of trans-Golgi network vesicle
cellular_component GO:0005829 cytosol
molecular_function GO:0005198 structural molecule activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0004591 oxoglutarate dehydrogenase (succinyl-transferring) activity
molecular_function GO:0030976 thiamine pyrophosphate binding
molecular_function GO:0005515 protein binding
molecular_function GO:0008536 Ran GTPase binding
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0008641 ubiquitin-like modifier activating enzyme activity
RNA-Seq Expression