Spo21767 (gene) | SpinachBase

Overview


Name	Spo21767
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Ribosome-inactivating protein PD-L1/PD-L2 (3.2.2.22) (rRNA N-glycosidase PD-L1/PD-L2)
Location	chr2 : 56905290 .. 56906229 (-)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGGAACAAAATCTTAAGGTGTGGCTTATTCTAGCAATATCAGCATGGGTCATCATCCTCCAACAAGTAAATGCAGTATCAGATGTAACCTTCGACCTTGAAACAGCTTCAAAAACAAAATACAACACCTTCCTAAGTGACATACGAACCAAAGTAAAGGATCCAAAACTAGTTTACGAGGGAATCCCAATGTTACCACCCCCAACAACACCAACAAAATACATTCTGATCGACCTAAAAGCCAAACAAAACGCTGCTACTACAATAACAGTAACACTTGCTCTTAGCAGAAACGACTTATACGTAGTCGCCTTCACTGATCAAACTGCACCAAATTTACGCGCACATTATTTCGCTGCTGATATTACTCCTGCTACTGTAAGGACTCTGTTTCCAACAGCAACAGAGCTTATTCCGATTGGTTATGGGAGTAGTTACCAGAATCTTGAAGGAAATGCTGGTATTCTTAGAGAAAATCTGCAGTTGGGATTTCAGCCATTAAAACAGTACATGGTGAATGTATATGGGAAGAGTATTCAGGCTACAGATTATCGGAAAAATGAAGCTAAATTCCTTCTTACTACTATCCAAATGGTTGCAGAGGCAGCAAGATTTAAGTATATTGAAGGGAAAGCTATTAATAGTTTTATTCCTGATTATAAAGTCCCGAGTTTAGAGACGAACTGGGAAAAGATATCTAAAGCTATTCGTTCTGCTGTTAAGAAGTTTACCCCGCCAATTGCGTTAGTATATGCTAATAATACGGCATGGGTAATTACTCAAGTTAGTGATATTAAGCCTGATCTTGGACTTCTTAAATATGTACCCTAATTAAGTAATTTAAGGTTATTTTTATATTTATCGTCTTTTAAGTTTCACGTATTTTTATTATTTTTCCCTTTATAAAATAAAATGTAATTTCGTATTTATCACCATA

mRNA sequence

ATGCAGGAACAAAATCTTAAGGTGTGGCTTATTCTAGCAATATCAGCATGGGTCATCATCCTCCAACAAGTAAATGCAGTATCAGATGTAACCTTCGACCTTGAAACAGCTTCAAAAACAAAATACAACACCTTCCTAAGTGACATACGAACCAAAGTAAAGGATCCAAAACTAGTTTACGAGGGAATCCCAATGTTACCACCCCCAACAACACCAACAAAATACATTCTGATCGACCTAAAAGCCAAACAAAACGCTGCTACTACAATAACAGTAACACTTGCTCTTAGCAGAAACGACTTATACGTAGTCGCCTTCACTGATCAAACTGCACCAAATTTACGCGCACATTATTTCGCTGCTGATATTACTCCTGCTACTGTAAGGACTCTGTTTCCAACAGCAACAGAGCTTATTCCGATTGGTTATGGGAGTAGTTACCAGAATCTTGAAGGAAATGCTGGTATTCTTAGAGAAAATCTGCAGTTGGGATTTCAGCCATTAAAACAGTACATGGTGAATGTATATGGGAAGAGTATTCAGGCTACAGATTATCGGAAAAATGAAGCTAAATTCCTTCTTACTACTATCCAAATGGTTGCAGAGGCAGCAAGATTTAAGTATATTGAAGGGAAAGCTATTAATAGTTTTATTCCTGATTATAAAGTCCCGAGTTTAGAGACGAACTGGGAAAAGATATCTAAAGCTATTCGTTCTGCTGTTAAGAAGTTTACCCCGCCAATTGCGTTAGTATATGCTAATAATACGGCATGGGTAATTACTCAAGTTAGTGATATTAAGCCTGATCTTGGACTTCTTAAATATGTACCCTAATTAAGTAATTTAAGGTTATTTTTATATTTATCGTCTTTTAAGTTTCACGTATTTTTATTATTTTTCCCTTTATAAAATAAAATGTAATTTCGTATTTATCACCATA

Coding sequence (CDS)

ATGCAGGAACAAAATCTTAAGGTGTGGCTTATTCTAGCAATATCAGCATGGGTCATCATCCTCCAACAAGTAAATGCAGTATCAGATGTAACCTTCGACCTTGAAACAGCTTCAAAAACAAAATACAACACCTTCCTAAGTGACATACGAACCAAAGTAAAGGATCCAAAACTAGTTTACGAGGGAATCCCAATGTTACCACCCCCAACAACACCAACAAAATACATTCTGATCGACCTAAAAGCCAAACAAAACGCTGCTACTACAATAACAGTAACACTTGCTCTTAGCAGAAACGACTTATACGTAGTCGCCTTCACTGATCAAACTGCACCAAATTTACGCGCACATTATTTCGCTGCTGATATTACTCCTGCTACTGTAAGGACTCTGTTTCCAACAGCAACAGAGCTTATTCCGATTGGTTATGGGAGTAGTTACCAGAATCTTGAAGGAAATGCTGGTATTCTTAGAGAAAATCTGCAGTTGGGATTTCAGCCATTAAAACAGTACATGGTGAATGTATATGGGAAGAGTATTCAGGCTACAGATTATCGGAAAAATGAAGCTAAATTCCTTCTTACTACTATCCAAATGGTTGCAGAGGCAGCAAGATTTAAGTATATTGAAGGGAAAGCTATTAATAGTTTTATTCCTGATTATAAAGTCCCGAGTTTAGAGACGAACTGGGAAAAGATATCTAAAGCTATTCGTTCTGCTGTTAAGAAGTTTACCCCGCCAATTGCGTTAGTATATGCTAATAATACGGCATGGGTAATTACTCAAGTTAGTGATATTAAGCCTGATCTTGGACTTCTTAAATATGTACCCTAA

Protein sequence

MQEQNLKVWLILAISAWVIILQQVNAVSDVTFDLETASKTKYNTFLSDIRTKVKDPKLVYEGIPMLPPPTTPTKYILIDLKAKQNAATTITVTLALSRNDLYVVAFTDQTAPNLRAHYFAADITPATVRTLFPTATELIPIGYGSSYQNLEGNAGILRENLQLGFQPLKQYMVNVYGKSIQATDYRKNEAKFLLTTIQMVAEAARFKYIEGKAINSFIPDYKVPSLETNWEKISKAIRSAVKKFTPPIALVYANNTAWVITQVSDIKPDLGLLKYVP

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo21767.1	Spo21767.1	mRNA

Homology

[Close]

BLAST of Spo21767.1 vs. NCBI nr
Match: gi|902201890|gb|KNA14122.1| (hypothetical protein SOVF_110520 [Spinacia oleracea])

HSP 1 Score: 542.3 bits (1396), Expect = 4.800e-151
Identity = 277/277 (100.00%), Postives = 277/277 (100.00%), Query Frame = 1

		  
Query: 1   MQEQNLKVWLILAISAWVIILQQVNAVSDVTFDLETASKTKYNTFLSDIRTKVKDPKLVY 60
           MQEQNLKVWLILAISAWVIILQQVNAVSDVTFDLETASKTKYNTFLSDIRTKVKDPKLVY
Sbjct: 1   MQEQNLKVWLILAISAWVIILQQVNAVSDVTFDLETASKTKYNTFLSDIRTKVKDPKLVY 60

Query: 61  EGIPMLPPPTTPTKYILIDLKAKQNAATTITVTLALSRNDLYVVAFTDQTAPNLRAHYFA 120
           EGIPMLPPPTTPTKYILIDLKAKQNAATTITVTLALSRNDLYVVAFTDQTAPNLRAHYFA
Sbjct: 61  EGIPMLPPPTTPTKYILIDLKAKQNAATTITVTLALSRNDLYVVAFTDQTAPNLRAHYFA 120

Query: 121 ADITPATVRTLFPTATELIPIGYGSSYQNLEGNAGILRENLQLGFQPLKQYMVNVYGKSI 180
           ADITPATVRTLFPTATELIPIGYGSSYQNLEGNAGILRENLQLGFQPLKQYMVNVYGKSI
Sbjct: 121 ADITPATVRTLFPTATELIPIGYGSSYQNLEGNAGILRENLQLGFQPLKQYMVNVYGKSI 180

Query: 181 QATDYRKNEAKFLLTTIQMVAEAARFKYIEGKAINSFIPDYKVPSLETNWEKISKAIRSA 240
           QATDYRKNEAKFLLTTIQMVAEAARFKYIEGKAINSFIPDYKVPSLETNWEKISKAIRSA
Sbjct: 181 QATDYRKNEAKFLLTTIQMVAEAARFKYIEGKAINSFIPDYKVPSLETNWEKISKAIRSA 240

Query: 241 VKKFTPPIALVYANNTAWVITQVSDIKPDLGLLKYVP 278
           VKKFTPPIALVYANNTAWVITQVSDIKPDLGLLKYVP
Sbjct: 241 VKKFTPPIALVYANNTAWVITQVSDIKPDLGLLKYVP 277

[Close]

BLAST of Spo21767.1 vs. NCBI nr
Match: gi|205361519|dbj|BAG71411.1| (type-1 ribosome-inactivating protein [Spinacia oleracea])

HSP 1 Score: 539.7 bits (1389), Expect = 3.100e-150
Identity = 276/277 (99.64%), Postives = 276/277 (99.64%), Query Frame = 1

		  
Query: 1   MQEQNLKVWLILAISAWVIILQQVNAVSDVTFDLETASKTKYNTFLSDIRTKVKDPKLVY 60
           MQEQNLKVWLILAISAWVIILQQVNAVSDVTFDLETASKTKYNTFLSDIRTKVKDPKLVY
Sbjct: 1   MQEQNLKVWLILAISAWVIILQQVNAVSDVTFDLETASKTKYNTFLSDIRTKVKDPKLVY 60

Query: 61  EGIPMLPPPTTPTKYILIDLKAKQNAATTITVTLALSRNDLYVVAFTDQTAPNLRAHYFA 120
           EGIPMLPPPTTPTKYILIDLKAKQNAATTITVTLALSRNDLYVVAFTDQTAPNLRAHYFA
Sbjct: 61  EGIPMLPPPTTPTKYILIDLKAKQNAATTITVTLALSRNDLYVVAFTDQTAPNLRAHYFA 120

Query: 121 ADITPATVRTLFPTATELIPIGYGSSYQNLEGNAGILRENLQLGFQPLKQYMVNVYGKSI 180
           ADITPATVRTLFPTATELIPIGYGSSYQNLEGNAGILRENLQLGFQPLKQYMVNVYGKSI
Sbjct: 121 ADITPATVRTLFPTATELIPIGYGSSYQNLEGNAGILRENLQLGFQPLKQYMVNVYGKSI 180

Query: 181 QATDYRKNEAKFLLTTIQMVAEAARFKYIEGKAINSFIPDYKVPSLETNWEKISKAIRSA 240
           QATDYRKNEAKFLLTTIQMVAEAARFKYIEGKAINSFIPDYKVPSL TNWEKISKAIRSA
Sbjct: 181 QATDYRKNEAKFLLTTIQMVAEAARFKYIEGKAINSFIPDYKVPSLGTNWEKISKAIRSA 240

Query: 241 VKKFTPPIALVYANNTAWVITQVSDIKPDLGLLKYVP 278
           VKKFTPPIALVYANNTAWVITQVSDIKPDLGLLKYVP
Sbjct: 241 VKKFTPPIALVYANNTAWVITQVSDIKPDLGLLKYVP 277

[Close]

BLAST of Spo21767.1 vs. NCBI nr
Match: gi|731354490|ref|XP_010688624.1| (PREDICTED: protein synthesis inhibitor PD-S2-like isoform X1 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 349.0 bits (894), Expect = 7.700e-93
Identity = 182/276 (65.94%), Postives = 221/276 (80.07%), Query Frame = 1

		  
Query: 4   QNLKV-WLILAISAWVIILQQVNAVSDVTFDLETASKTKYNTFLSDIRTKVKDPKLVYEG 63
           +N+K  WL+LAIS   ++LQ+V A +DVTFDLETASKTKY TFLS++R  VKD KLVYEG
Sbjct: 7   ENMKARWLVLAISIIWVVLQEVYA-ADVTFDLETASKTKYGTFLSNLRNNVKDSKLVYEG 66

Query: 64  IPMLPPPTTPTKYILIDLKAKQNAATTITVTLALSRNDLYVVAFTDQTAPNLRAHYFAAD 123
           IPMLP P+ P KY+L +LKAK+ A T IT+TLA+S+NDLYVVAFTDQ A  LRAHYF  D
Sbjct: 67  IPMLPAPSKPAKYLLAELKAKK-AGTDITITLAVSKNDLYVVAFTDQVAGKLRAHYFP-D 126

Query: 124 ITPATVRTLFPTATELIPIGYGSSYQNLEGNAGILRENLQLGFQPLKQYMVNVYGKSIQA 183
           I+ AT + +FPTAT+ I IGY S+Y ++EG AG  R N QLGF  LK+YM+NVYGK++Q 
Sbjct: 127 ISLATAKAIFPTATQYIQIGYTSNYVSIEGAAGSNRVNFQLGFVKLKEYMLNVYGKNVQD 186

Query: 184 TDYRKNEAKFLLTTIQMVAEAARFKYIEGKAINSFIPDYKVPSLETNWEKISKAIRSAVK 243
           +DYR++EA+FLL  IQMVAEAARFKY+E KAIN+F+PDYKVPSLE NW KIS+ IR AVK
Sbjct: 187 SDYRRSEARFLLAAIQMVAEAARFKYVESKAINNFVPDYKVPSLENNWSKISEGIRKAVK 246

Query: 244 K-FTPPIALVYANNTAWVITQVSDIKPDLGLLKYVP 278
           K   PPI LV A+N  W + QVSDIKPD+G+L YVP
Sbjct: 247 KVINPPIELVNASNGKWTVNQVSDIKPDMGILSYVP 279

[Close]

BLAST of Spo21767.1 vs. NCBI nr
Match: gi|731354492|ref|XP_010688625.1| (PREDICTED: antiviral protein alpha-like isoform X2 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 347.8 bits (891), Expect = 1.700e-92
Identity = 180/270 (66.67%), Postives = 217/270 (80.37%), Query Frame = 1

		  
Query: 9   WLILAISAWVIILQQVNAVSDVTFDLETASKTKYNTFLSDIRTKVKDPKLVYEGIPMLPP 68
           WL+LAIS   ++LQ+V A +DVTFDLETASKTKY TFLS++R  VKD KLVYEGIPMLP 
Sbjct: 5   WLVLAISIIWVVLQEVYA-ADVTFDLETASKTKYGTFLSNLRNNVKDSKLVYEGIPMLPA 64

Query: 69  PTTPTKYILIDLKAKQNAATTITVTLALSRNDLYVVAFTDQTAPNLRAHYFAADITPATV 128
           P+ P KY+L +LKAK+ A T IT+TLA+S+NDLYVVAFTDQ A  LRAHYF  DI+ AT 
Sbjct: 65  PSKPAKYLLAELKAKK-AGTDITITLAVSKNDLYVVAFTDQVAGKLRAHYFP-DISLATA 124

Query: 129 RTLFPTATELIPIGYGSSYQNLEGNAGILRENLQLGFQPLKQYMVNVYGKSIQATDYRKN 188
           + +FPTAT+ I IGY S+Y ++EG AG  R N QLGF  LK+YM+NVYGK++Q +DYR++
Sbjct: 125 KAIFPTATQYIQIGYTSNYVSIEGAAGSNRVNFQLGFVKLKEYMLNVYGKNVQDSDYRRS 184

Query: 189 EAKFLLTTIQMVAEAARFKYIEGKAINSFIPDYKVPSLETNWEKISKAIRSAVKK-FTPP 248
           EA+FLL  IQMVAEAARFKY+E KAIN+F+PDYKVPSLE NW KIS+ IR AVKK   PP
Sbjct: 185 EARFLLAAIQMVAEAARFKYVESKAINNFVPDYKVPSLENNWSKISEGIRKAVKKVINPP 244

Query: 249 IALVYANNTAWVITQVSDIKPDLGLLKYVP 278
           I LV A+N  W + QVSDIKPD+G+L YVP
Sbjct: 245 IELVNASNGKWTVNQVSDIKPDMGILSYVP 271

[Close]

BLAST of Spo21767.1 vs. NCBI nr
Match: gi|1405841|emb|CAA59952.1| (Betavulgin [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 344.0 bits (881), Expect = 2.500e-91
Identity = 178/270 (65.93%), Postives = 214/270 (79.26%), Query Frame = 1

		  
Query: 9   WLILAISAWVIILQQVNAVSDVTFDLETASKTKYNTFLSDIRTKVKDPKLVYEGIPMLPP 68
           WL+LAIS   ++LQ V   +DVTFDLETASKTKY TFLS++R  VKD KLVYEGIPMLP 
Sbjct: 5   WLVLAISIIWVVLQGVVYAADVTFDLETASKTKYGTFLSNLRNIVKDSKLVYEGIPMLPA 64

Query: 69  PTTPTKYILIDLKAKQNAATTITVTLALSRNDLYVVAFTDQTAPNLRAHYFAADITPATV 128
           P  P KY+L +LKAK+ A T IT+TLA+S+NDLYVVAFTDQ A  LRAHYF  DI+ AT 
Sbjct: 65  PIKPAKYLLAELKAKK-AGTDITITLAVSKNDLYVVAFTDQVAGKLRAHYFP-DISLATA 124

Query: 129 RTLFPTATELIPIGYGSSYQNLEGNAGILRENLQLGFQPLKQYMVNVYGKSIQATDYRKN 188
           + +FPTAT+ I IGY S+Y ++EG AG  R N QLGF  LK+YM+NVYGK++Q +DYR++
Sbjct: 125 KAIFPTATQYIQIGYTSNYVSIEGAAGSNRVNFQLGFVKLKEYMLNVYGKNVQDSDYRRS 184

Query: 189 EAKFLLTTIQMVAEAARFKYIEGKAINSFIPDYKVPSLETNWEKISKAIRSAVKK-FTPP 248
           EA+FLL  IQMVAEAARFKY+E KAIN+ +PDYKVPSLE NW KIS+ IR AVKK  +PP
Sbjct: 185 EARFLLAAIQMVAEAARFKYVESKAINNVVPDYKVPSLENNWSKISEGIRKAVKKVISPP 244

Query: 249 IALVYANNTAWVITQVSDIKPDLGLLKYVP 278
           I LV A+N  W + QVSDIKPD+G+L YVP
Sbjct: 245 IELVNASNGKWTVNQVSDIKPDMGILSYVP 272

[Close]

BLAST of Spo21767.1 vs. UniProtKB/TrEMBL
Match: A0A0K9R5Q5_SPIOL (rRNA N-glycosidase OS=Spinacia oleracea GN=SOVF_110520 PE=3 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 3.300e-151
Identity = 277/277 (100.00%), Postives = 277/277 (100.00%), Query Frame = 1

		  
Query: 1   MQEQNLKVWLILAISAWVIILQQVNAVSDVTFDLETASKTKYNTFLSDIRTKVKDPKLVY 60
           MQEQNLKVWLILAISAWVIILQQVNAVSDVTFDLETASKTKYNTFLSDIRTKVKDPKLVY
Sbjct: 1   MQEQNLKVWLILAISAWVIILQQVNAVSDVTFDLETASKTKYNTFLSDIRTKVKDPKLVY 60

Query: 61  EGIPMLPPPTTPTKYILIDLKAKQNAATTITVTLALSRNDLYVVAFTDQTAPNLRAHYFA 120
           EGIPMLPPPTTPTKYILIDLKAKQNAATTITVTLALSRNDLYVVAFTDQTAPNLRAHYFA
Sbjct: 61  EGIPMLPPPTTPTKYILIDLKAKQNAATTITVTLALSRNDLYVVAFTDQTAPNLRAHYFA 120

Query: 121 ADITPATVRTLFPTATELIPIGYGSSYQNLEGNAGILRENLQLGFQPLKQYMVNVYGKSI 180
           ADITPATVRTLFPTATELIPIGYGSSYQNLEGNAGILRENLQLGFQPLKQYMVNVYGKSI
Sbjct: 121 ADITPATVRTLFPTATELIPIGYGSSYQNLEGNAGILRENLQLGFQPLKQYMVNVYGKSI 180

Query: 181 QATDYRKNEAKFLLTTIQMVAEAARFKYIEGKAINSFIPDYKVPSLETNWEKISKAIRSA 240
           QATDYRKNEAKFLLTTIQMVAEAARFKYIEGKAINSFIPDYKVPSLETNWEKISKAIRSA
Sbjct: 181 QATDYRKNEAKFLLTTIQMVAEAARFKYIEGKAINSFIPDYKVPSLETNWEKISKAIRSA 240

Query: 241 VKKFTPPIALVYANNTAWVITQVSDIKPDLGLLKYVP 278
           VKKFTPPIALVYANNTAWVITQVSDIKPDLGLLKYVP
Sbjct: 241 VKKFTPPIALVYANNTAWVITQVSDIKPDLGLLKYVP 277

[Close]

BLAST of Spo21767.1 vs. UniProtKB/TrEMBL
Match: B5MET5_SPIOL (rRNA N-glycosidase OS=Spinacia oleracea GN=RIP PE=2 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 2.200e-150
Identity = 276/277 (99.64%), Postives = 276/277 (99.64%), Query Frame = 1

		  
Query: 1   MQEQNLKVWLILAISAWVIILQQVNAVSDVTFDLETASKTKYNTFLSDIRTKVKDPKLVY 60
           MQEQNLKVWLILAISAWVIILQQVNAVSDVTFDLETASKTKYNTFLSDIRTKVKDPKLVY
Sbjct: 1   MQEQNLKVWLILAISAWVIILQQVNAVSDVTFDLETASKTKYNTFLSDIRTKVKDPKLVY 60

Query: 61  EGIPMLPPPTTPTKYILIDLKAKQNAATTITVTLALSRNDLYVVAFTDQTAPNLRAHYFA 120
           EGIPMLPPPTTPTKYILIDLKAKQNAATTITVTLALSRNDLYVVAFTDQTAPNLRAHYFA
Sbjct: 61  EGIPMLPPPTTPTKYILIDLKAKQNAATTITVTLALSRNDLYVVAFTDQTAPNLRAHYFA 120

Query: 121 ADITPATVRTLFPTATELIPIGYGSSYQNLEGNAGILRENLQLGFQPLKQYMVNVYGKSI 180
           ADITPATVRTLFPTATELIPIGYGSSYQNLEGNAGILRENLQLGFQPLKQYMVNVYGKSI
Sbjct: 121 ADITPATVRTLFPTATELIPIGYGSSYQNLEGNAGILRENLQLGFQPLKQYMVNVYGKSI 180

Query: 181 QATDYRKNEAKFLLTTIQMVAEAARFKYIEGKAINSFIPDYKVPSLETNWEKISKAIRSA 240
           QATDYRKNEAKFLLTTIQMVAEAARFKYIEGKAINSFIPDYKVPSL TNWEKISKAIRSA
Sbjct: 181 QATDYRKNEAKFLLTTIQMVAEAARFKYIEGKAINSFIPDYKVPSLGTNWEKISKAIRSA 240

Query: 241 VKKFTPPIALVYANNTAWVITQVSDIKPDLGLLKYVP 278
           VKKFTPPIALVYANNTAWVITQVSDIKPDLGLLKYVP
Sbjct: 241 VKKFTPPIALVYANNTAWVITQVSDIKPDLGLLKYVP 277

[Close]

BLAST of Spo21767.1 vs. UniProtKB/TrEMBL
Match: A0A0J8EHU6_BETVU (rRNA N-glycosidase OS=Beta vulgaris subsp. vulgaris GN=BVRB_9g202430 PE=3 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 1.200e-92
Identity = 180/270 (66.67%), Postives = 217/270 (80.37%), Query Frame = 1

		  
Query: 9   WLILAISAWVIILQQVNAVSDVTFDLETASKTKYNTFLSDIRTKVKDPKLVYEGIPMLPP 68
           WL+LAIS   ++LQ+V A +DVTFDLETASKTKY TFLS++R  VKD KLVYEGIPMLP 
Sbjct: 5   WLVLAISIIWVVLQEVYA-ADVTFDLETASKTKYGTFLSNLRNNVKDSKLVYEGIPMLPA 64

Query: 69  PTTPTKYILIDLKAKQNAATTITVTLALSRNDLYVVAFTDQTAPNLRAHYFAADITPATV 128
           P+ P KY+L +LKAK+ A T IT+TLA+S+NDLYVVAFTDQ A  LRAHYF  DI+ AT 
Sbjct: 65  PSKPAKYLLAELKAKK-AGTDITITLAVSKNDLYVVAFTDQVAGKLRAHYFP-DISLATA 124

Query: 129 RTLFPTATELIPIGYGSSYQNLEGNAGILRENLQLGFQPLKQYMVNVYGKSIQATDYRKN 188
           + +FPTAT+ I IGY S+Y ++EG AG  R N QLGF  LK+YM+NVYGK++Q +DYR++
Sbjct: 125 KAIFPTATQYIQIGYTSNYVSIEGAAGSNRVNFQLGFVKLKEYMLNVYGKNVQDSDYRRS 184

Query: 189 EAKFLLTTIQMVAEAARFKYIEGKAINSFIPDYKVPSLETNWEKISKAIRSAVKK-FTPP 248
           EA+FLL  IQMVAEAARFKY+E KAIN+F+PDYKVPSLE NW KIS+ IR AVKK   PP
Sbjct: 185 EARFLLAAIQMVAEAARFKYVESKAINNFVPDYKVPSLENNWSKISEGIRKAVKKVINPP 244

Query: 249 IALVYANNTAWVITQVSDIKPDLGLLKYVP 278
           I LV A+N  W + QVSDIKPD+G+L YVP
Sbjct: 245 IELVNASNGKWTVNQVSDIKPDMGILSYVP 271

[Close]

BLAST of Spo21767.1 vs. UniProtKB/TrEMBL
Match: Q39418_BETVU (rRNA N-glycosidase OS=Beta vulgaris subsp. vulgaris GN=betavulgin PE=2 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 1.700e-91
Identity = 178/270 (65.93%), Postives = 214/270 (79.26%), Query Frame = 1

		  
Query: 9   WLILAISAWVIILQQVNAVSDVTFDLETASKTKYNTFLSDIRTKVKDPKLVYEGIPMLPP 68
           WL+LAIS   ++LQ V   +DVTFDLETASKTKY TFLS++R  VKD KLVYEGIPMLP 
Sbjct: 5   WLVLAISIIWVVLQGVVYAADVTFDLETASKTKYGTFLSNLRNIVKDSKLVYEGIPMLPA 64

Query: 69  PTTPTKYILIDLKAKQNAATTITVTLALSRNDLYVVAFTDQTAPNLRAHYFAADITPATV 128
           P  P KY+L +LKAK+ A T IT+TLA+S+NDLYVVAFTDQ A  LRAHYF  DI+ AT 
Sbjct: 65  PIKPAKYLLAELKAKK-AGTDITITLAVSKNDLYVVAFTDQVAGKLRAHYFP-DISLATA 124

Query: 129 RTLFPTATELIPIGYGSSYQNLEGNAGILRENLQLGFQPLKQYMVNVYGKSIQATDYRKN 188
           + +FPTAT+ I IGY S+Y ++EG AG  R N QLGF  LK+YM+NVYGK++Q +DYR++
Sbjct: 125 KAIFPTATQYIQIGYTSNYVSIEGAAGSNRVNFQLGFVKLKEYMLNVYGKNVQDSDYRRS 184

Query: 189 EAKFLLTTIQMVAEAARFKYIEGKAINSFIPDYKVPSLETNWEKISKAIRSAVKK-FTPP 248
           EA+FLL  IQMVAEAARFKY+E KAIN+ +PDYKVPSLE NW KIS+ IR AVKK  +PP
Sbjct: 185 EARFLLAAIQMVAEAARFKYVESKAINNVVPDYKVPSLENNWSKISEGIRKAVKKVISPP 244

Query: 249 IALVYANNTAWVITQVSDIKPDLGLLKYVP 278
           I LV A+N  W + QVSDIKPD+G+L YVP
Sbjct: 245 IELVNASNGKWTVNQVSDIKPDMGILSYVP 272

[Close]

BLAST of Spo21767.1 vs. UniProtKB/TrEMBL
Match: Q5QHN9_BETVU (Ribosome-inactivating protein BE27 (Fragment) OS=Beta vulgaris subsp. vulgaris PE=4 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 1.200e-87
Identity = 169/251 (67.33%), Postives = 202/251 (80.48%), Query Frame = 1

		  
Query: 28  SDVTFDLETASKTKYNTFLSDIRTKVKDPKLVYEGIPMLPPPTTPTKYILIDLKAKQNAA 87
           +DVTFDLETASKTKY TFLS++R  VKD KLVYEGIPMLP P  P KY+L +LKAK+ A 
Sbjct: 1   ADVTFDLETASKTKYGTFLSNLRNIVKDSKLVYEGIPMLPAPIKPAKYLLAELKAKK-AG 60

Query: 88  TTITVTLALSRNDLYVVAFTDQTAPNLRAHYFAADITPATVRTLFPTATELIPIGYGSSY 147
           T IT+TLA+S+NDLYVVAFTDQ A  LRAHYF  DI+ AT + +FPTAT+ I IGY S+Y
Sbjct: 61  TDITITLAVSKNDLYVVAFTDQVAGKLRAHYFP-DISLATAKAIFPTATQYIQIGYTSNY 120

Query: 148 QNLEGNAGILRENLQLGFQPLKQYMVNVYGKSIQATDYRKNEAKFLLTTIQMVAEAARFK 207
            ++EG AG  R N QLGF  LK+YM+NVYGK++Q +DYR++EA+FLL  IQMVAEAARFK
Sbjct: 121 VSIEGAAGSNRVNFQLGFVKLKEYMLNVYGKNVQDSDYRRSEARFLLAAIQMVAEAARFK 180

Query: 208 YIEGKAINSFIPDYKVPSLETNWEKISKAIRSAVKK-FTPPIALVYANNTAWVITQVSDI 267
           Y+E KAIN+ +PDYKVPSLE NW KIS+ IR AVKK  +PPI LV A+N  W + QVSDI
Sbjct: 181 YVESKAINNVVPDYKVPSLENNWSKISEGIRKAVKKVISPPIELVNASNGKWTVNQVSDI 240

Query: 268 KPDLGLLKYVP 278
           KPD+G+L YVP
Sbjct: 241 KPDMGILSYVP 249

[Close]

BLAST of Spo21767.1 vs. ExPASy Swiss-Prot
Match: RIP2_PHYDI (Protein synthesis inhibitor PD-S2 OS=Phytolacca dioica PE=1 SV=2)

HSP 1 Score: 172.9 bits (437), Expect = 4.800e-42
Identity = 111/262 (42.37%), Postives = 151/262 (57.63%), Query Frame = 1

		  
Query: 27  VSDVTFDLETASKTKYNTFLSDIRTKVKDPKLVYEGIPMLPPPTTPTKYILIDLKAKQNA 86
           VS +TFD+ +A+ +KY TFL  +R + KDP L   GIPMLP      KY+L++L AK ++
Sbjct: 1   VSTITFDVGSATISKYTTFLESLRNQAKDPSLKCYGIPMLPNTNPNPKYLLVELNAKLSS 60

Query: 87  ATTITVTLALSRNDLYVVAFTDQTAPNLRAHYFAADITPATVR-----TLFPTATELIP- 146
               ++TL L R++LYV+ ++D      R H F  DI+  T R     TL P A+  +  
Sbjct: 61  GEVKSITLMLRRHNLYVMGYSDPYDNKCRYHIF-KDISSTTERKDVETTLCPNASSRVSK 120

Query: 147 -IGYGSSYQNLEGNAGILRENLQLGFQPLKQYMVNVYGKSIQATDYRKNEAKFLLTTIQM 206
            I Y SSY  LE  AG  R  +QLG Q L   +  + G     TD  K EA+FLL  IQM
Sbjct: 121 NISYDSSYPALENKAGRSRSQVQLGIQILNSDIGKISGVK-SFTD--KTEAEFLLVAIQM 180

Query: 207 VAEAARFKYIEGKA----INSFIPDYKVPSLETNWEKISKAIRSAVK-KFTPPIALVYAN 266
           V+EA RFKYIE +       +F P+ KV +LE  W KIS AI  A    F+ P+ L  A+
Sbjct: 181 VSEATRFKYIENQVKTNFNRAFYPNAKVLNLEETWGKISMAIHGAKNGAFSKPLELQNAD 240

Query: 267 NTAWVITQVSDIKPDLGLLKYV 277
            + W++ +V +IKPD+ LLKYV
Sbjct: 241 GSKWIVLRVDEIKPDVALLKYV 258

[Close]

BLAST of Spo21767.1 vs. ExPASy Swiss-Prot
Match: RIPA_PHYAM (Antiviral protein alpha OS=Phytolacca americana PE=1 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 9.900e-40
Identity = 112/284 (39.44%), Postives = 158/284 (55.63%), Query Frame = 1

		  
Query: 6   LKVWLILAISAWVIILQQVN-AVSDVTFDLETASKTKYNTFLSDIRTKVKDPKLVYEGIP 65
           + V +++ + +W+I+      A++ +TFD+  A+  KY TF+  I  + KDP L   GIP
Sbjct: 3   MMVVVVVMMLSWLILKPPSTWAINTITFDVGNATINKYATFMKSIHNQAKDPTLKCYGIP 62

Query: 66  MLPPPTTPTKYILIDLKAKQNAATTITVTLALSRNDLYVVAFTDQTAPNLRAHYFAADIT 125
           MLP      KY+L+ L+     ++  T+TL L RN+LYV+ + D      R H F  DI+
Sbjct: 63  MLPNTNLTPKYLLVTLQD----SSLKTITLMLKRNNLYVMGYADTYNGKCRYHIFK-DIS 122

Query: 126 PATVR-----TLFPTATELIP--IGYGSSYQNLEGNAGILRENLQLGFQPLKQYMVNVYG 185
             T R     TL P  +  +   I Y SSY  LE   G  R  +QLG Q L   +  +YG
Sbjct: 123 NTTERNDVMTTLCPNPSSRVGKNINYDSSYPALEKKVGRPRSQVQLGIQILNSGIGKIYG 182

Query: 186 KSIQATDYRKNEAKFLLTTIQMVAEAARFKYIEGKA----INSFIPDYKVPSLETNWEKI 245
                +   K EA+FLL  IQMV+EAARFKYIE +       +F P+ KV +LE +W KI
Sbjct: 183 VD---SFTEKTEAEFLLVAIQMVSEAARFKYIENQVKTNFNRAFYPNAKVLNLEESWGKI 242

Query: 246 SKAIRSAVK-KFTPPIALVYANNTAWVITQVSDIKPDLGLLKYV 277
           S AI +A     T P+ L  AN + W++ +V DI+PD+GLLKYV
Sbjct: 243 STAIHNAKNGALTSPLELKNANGSKWIVLRVDDIEPDVGLLKYV 278

[Close]

BLAST of Spo21767.1 vs. ExPASy Swiss-Prot
Match: RIPL2_PHYDI (Ribosome-inactivating protein PD-L3/PD-L4 OS=Phytolacca dioica PE=1 SV=2)

HSP 1 Score: 162.9 bits (411), Expect = 4.900e-39
Identity = 108/261 (41.38%), Postives = 148/261 (56.70%), Query Frame = 1

		  
Query: 27  VSDVTFDLETASKTKYNTFLSDIRTKVKDPKLVYEGIPMLPPPTTPTKYILIDLKAKQNA 86
           V+ +TFD+  A+  KY TF+  +R + KDP L   GIPMLP      KY+L+ L+     
Sbjct: 1   VNTITFDVGNATINKYATFMESLRNEAKDPTLKCYGIPMLPDSNLTPKYVLVKLQ----D 60

Query: 87  ATTITVTLALSRNDLYVVAFTDQTAPNLRAHYF---AADITPATVRTLFPTAT--ELIPI 146
           A++ T+TL L RN+LYV+ ++D      R H F   ++  +     TL P +   E   I
Sbjct: 61  ASSKTITLMLRRNNLYVMGYSDLYNGKCRYHIFNDISSTESTDVENTLCPNSNSREKKAI 120

Query: 147 GYGSSYQNLEGNAGI-LRENLQLGFQPLKQYMVNVYGKSIQATDYRKNEAKFLLTTIQMV 206
            Y S Y  L+  AG+  R  +QLG Q L   +  + G S   T   K EA+FLL  IQMV
Sbjct: 121 NYNSQYSTLQNKAGVSSRSQVQLGIQILNSDIGKISGVS---TFTDKTEAEFLLVAIQMV 180

Query: 207 AEAARFKYIEGKA----INSFIPDYKVPSLETNWEKISKAIRSAVK-KFTPPIALVYANN 266
           +EAARFKYIE +       +F P+ KV SLE NW KIS AI +A     T P+ L  A++
Sbjct: 181 SEAARFKYIENQVKTNFNRAFNPNPKVLSLEENWGKISLAIHNAKNGALTSPLELKNADD 240

Query: 267 TAWVITQVSDIKPDLGLLKYV 277
           T W++ +V +IKPD+GLL YV
Sbjct: 241 TKWIVLRVDEIKPDMGLLNYV 254

[Close]

BLAST of Spo21767.1 vs. ExPASy Swiss-Prot
Match: RIPL1_PHYDI (Ribosome-inactivating protein PD-L1/PD-L2 OS=Phytolacca dioica PE=1 SV=2)

HSP 1 Score: 162.2 bits (409), Expect = 8.400e-39
Identity = 109/263 (41.44%), Postives = 148/263 (56.27%), Query Frame = 1

		  
Query: 27  VSDVTFDLETASKTKYNTFLSDIRTKVKDPKLVYEGIPMLPPPTTPTKYILIDLKAKQNA 86
           ++ +T+D    +  KY TF+  +R + KDP L   GIPMLP  ++  KY+L+ L+     
Sbjct: 1   INTITYDAGNTTINKYATFMESLRNEAKDPSLQCYGIPMLPNNSSTIKYLLVKLQ----G 60

Query: 87  ATTITVTLALSRNDLYVVAFTDQTAPNLRAHYFAADITPATVRT-------LFPTATELI 146
           A+  T+TL L RN+LYV+ ++D    N R H F  DIT  T RT          ++ +  
Sbjct: 61  ASQKTITLMLRRNNLYVMGYSDPFNGNCRYHIF-NDIT-GTERTNVENTLCSSSSSRDAK 120

Query: 147 PIGYGSSYQNLEGNAGI-LRENLQLGFQPLKQYMVNVYGKSIQATDYRKNEAKFLLTTIQ 206
           PI Y S Y  LE  A +  R  +QLG Q L   +  + G+S   TD  K EAKFLL  IQ
Sbjct: 121 PINYNSLYSTLEKKAEVNSRSQVQLGIQILSSDIGKISGQS-SFTD--KTEAKFLLVAIQ 180

Query: 207 MVAEAARFKYIEGKAINSFIPDY----KVPSLETNWEKISKAIRSAVKKFTP-PIALVYA 266
           MV+EAARFKYIE +   +F  D+    K+  LE NW KIS AI  A     P P+ L  A
Sbjct: 181 MVSEAARFKYIENQVKTNFNRDFSPNDKILDLEENWGKISTAIHDATNGALPKPLELKNA 240

Query: 267 NNTAWVITQVSDIKPDLGLLKYV 277
           + T W++ +V +IKPD+GLL YV
Sbjct: 241 DGTKWIVLRVDEIKPDMGLLNYV 254

[Close]

BLAST of Spo21767.1 vs. ExPASy Swiss-Prot
Match: RIPS_PHYAM (Antiviral protein S OS=Phytolacca americana PE=1 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 1.900e-38
Identity = 107/262 (40.84%), Postives = 145/262 (55.34%), Query Frame = 1

		  
Query: 27  VSDVTFDLETASKTKYNTFLSDIRTKVKDPKLVYEGIPMLPPPTTPTKYILIDLKAKQNA 86
           ++ +TFD   A+  KY TF+  +R + KDP L   GIPMLP   +  KY+L+ L+     
Sbjct: 1   INTITFDAGNATINKYATFMESLRNEAKDPSLKCYGIPMLPNTNSTIKYLLVKLQG---- 60

Query: 87  ATTITVTLALSRNDLYVVAFTDQTAPNLRAHYFAADITPATV----RTLFPTATELI--P 146
           A+  T+TL L RN+LYV+ ++D      R H F  DI          TL P++   +  P
Sbjct: 61  ASLKTITLMLRRNNLYVMGYSDPYDNKCRYHIFN-DIKGTEYSDVENTLCPSSNPRVAKP 120

Query: 147 IGYGSSYQNLEGNAGILREN-LQLGFQPLKQYMVNVYGKSIQATDYRKNEAKFLLTTIQM 206
           I Y   Y  LE  AG+   N +QLG Q L   +  + G   Q +   K EAKFLL  IQM
Sbjct: 121 INYNGLYPTLEKKAGVTSRNEVQLGIQILSSDIGKISG---QGSFTEKIEAKFLLVAIQM 180

Query: 207 VAEAARFKYIEGKAINSFIPDY----KVPSLETNWEKISKAIRSAVKKFTP-PIALVYAN 266
           V+EAARFKYIE +   +F  D+    KV  LE NW KIS AI ++     P P+ L  A+
Sbjct: 181 VSEAARFKYIENQVKTNFNRDFSPNDKVLDLEENWGKISTAIHNSKNGALPKPLELKNAD 240

Query: 267 NTAWVITQVSDIKPDLGLLKYV 277
            T W++ +V +IKPD+GLL YV
Sbjct: 241 GTKWIVLRVDEIKPDVGLLNYV 254

The following BLAST results are available for this feature:

BLAST of Spo21767.1 vs. NCBI nr
BLAST of Spo21767.1 vs. UniProtKB/TrEMBL
BLAST of Spo21767.1 vs. ExPASy Swiss-Prot
BLAST of Spo21767.1 vs. TAIR (Arabidopsis)

BLAST of Spo21767.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5

Position :

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x 1


Match Name	E-value	Identity	Description
gi\|902201890\|gb\|KNA14122.1\|	4.8e-151	100.	hypothetical protein SOVF_1105...	[more]
gi\|205361519\|dbj\|BAG71411.1\|	3.1e-150	99.6	type-1 ribosome-inactivating p...	[more]
gi\|731354490\|ref\|XP_010688624.1\|	7.7e-93	65.9	PREDICTED: protein synthesis i...	[more]
gi\|731354492\|ref\|XP_010688625.1\|	1.7e-92	66.6	PREDICTED: antiviral protein a...	[more]
gi\|1405841\|emb\|CAA59952.1\|	2.5e-91	65.9	Betavulgin [Beta vulgaris subs...	[more]

BLAST of Spo21767.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5

Position :

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x 1


Match Name	E-value	Identity	Description
A0A0K9R5Q5_SPIOL	3.3e-151	100.	rRNA N-glycosidase OS=Spinacia...	[more]
B5MET5_SPIOL	2.2e-150	99.6	rRNA N-glycosidase OS=Spinacia...	[more]
A0A0J8EHU6_BETVU	1.2e-92	66.6	rRNA N-glycosidase OS=Beta vul...	[more]
Q39418_BETVU	1.7e-91	65.9	rRNA N-glycosidase OS=Beta vul...	[more]
Q5QHN9_BETVU	1.2e-87	67.3	Ribosome-inactivating protein ...	[more]

BLAST of Spo21767.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5

Position :

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x 1


Match Name	E-value	Identity	Description
RIP2_PHYDI	4.8e-42	42.3	Protein synthesis inhibitor PD...	[more]
RIPA_PHYAM	9.9e-40	39.4	Antiviral protein alpha OS=Phy...	[more]
RIPL2_PHYDI	4.9e-39	41.3	Ribosome-inactivating protein ...	[more]
RIPL1_PHYDI	8.4e-39	41.4	Ribosome-inactivating protein ...	[more]
RIPS_PHYAM	1.9e-38	40.8	Antiviral protein S OS=Phytola...	[more]

BLAST of Spo21767.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 0

Position :

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x 1


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 38..237 score: 1.1
IPR001574	Ribosome-inactivating protein	unknown	SSF56371	Ribosome inactivating proteins (RIP)	coord: 30..276 score: 1.44
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10		coord: 29..204 score: 2.4
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10		coord: 206..276 score: 4.8
IPR017989	Ribosome-inactivating protein subgroup	PRINTS	PR00396	SHIGARICIN	coord: 40..53 score: 7.0E-26coord: 193..213 score: 7.0E-26coord: 221..238 score: 7.0E-26coord: 91..106 score: 7.0E-26coord: 158..172 score: 7.0

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0017148	negative regulation of translation
biological_process	GO:0050896	response to stimulus
biological_process	GO:0008152	metabolic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0006468	protein phosphorylation
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0016757	transferase activity, transferring glycosyl groups
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo01650	0.71	Barchart \| Table
Spo26539	0.71	Barchart \| Table
Spo14335	0.70	Barchart \| Table
Spo28168	0.70	Barchart \| Table
Spo10023	0.70	Barchart \| Table
Spo12171	0.69	Barchart \| Table
Spo02143	0.67	Barchart \| Table
Spo00760	0.66	Barchart \| Table