Spo21771 (gene) | SpinachBase

Overview


Name	Spo21771
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Ribosome-inactivating protein PD-L3/PD-L4 (3.2.2.22) (rRNA N-glycosidase PD-L3/PD-L4)
Location	chr2 : 56991836 .. 56996053 (-)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTATTTAAGGAAGATAGCAAAACATATATTGGGGGTATCCTTTCACTAAATTATCACATATCGATCGTTATTCTTTATACTAGTCATTTTATACAACGCCTCAACAATTCGGTAAGTTTCTCTATATTCAACTATGCGTTTCTCTTCATACTATCCGTACTTGTTAATGGTAAGTAGAGATGGTTGGTGGGTAGGACTCAGTCCACCTTGATACAGTTTATAGTGGATCACGTGGGTCGGATCTACTTTTGACTCATGACACAACCTGTCCAATCCGGGTATTTAATTTTTTGATGTGTCGTAGGTTGAGTTTTTTTAACACAACTTATTTCGACCCACCCTATCGGACCCGACACAACATATCACGGCACACCCGACCCATCCAACCCAACACATGAATCTAACCTACCCAACACTCGAACTCAACCCACCAAACCCATCCTTATTTAAAGCATTTTGTATGTTTGAAATGCTAACAAATTCTAATTTTATTGTTTAAATATACATTAACCACATTTGTGTGAGTATTTGAATAACATAAAAATTAAGTAATTTAAGTTCATATAAAATTTATAACTTTTTAACGCTTAAGTAGTCACCCAAACCCACATGACCTACATCAACGCCTAAAACTCGTTTAAACCCACCAAACCCGGCCACCTGAACCATTGGAGTGACACCAAGCCTCAAAATCTGTCGTAACCCACCAAACTCATAAGACCCACTCGACCCACACAACCCATGATCCACGTAATCCACGACCGACCCCCATCATGTATTGTGTCCATTTTTTCCGACACAACCTACGACTTGACCCATCCTTAGTTTAACTGAGGTACGTGGGTCGTGTGTCAAATATCCTCGATCCACGACCCACCAAACCCACCCATGACCTACTCAACCAACCATGTTGGCCATCTCTAATTGTAAGTAGCATGATCCCCCCCCCCCTTCCTTATGCAAGCTTGGAACGATGTATTAAAAACAAAGGCCTTTGTGGCAAATAAGCCCAAGACTTATCTTTTAAGTGCAAACCAGTCCTCCTACTTTTAATTGAAGCAAATCAGTCCCTTACGTTTTGTAATTAGTGCAATCCACATCAAAATAAAATTTTCAATCAATCTAAACCTGAAATCCACTATACAACTTTAATTTCGCCAGAAAACAACTCCAATTTTAACGCTATAAATATTTTGTACAATATACGTTGTATATAAAATTTTAATAATTACAAAATCCTAAATTCACAAAAAAAAAAAAAAAAAAAAAGAAGAGAGAGAGAAAGAAAAGAACTGCAGGAAGCAGCAAATCGCAGGAAACAGCAACAACGAGCTCTTAACTATACGAATTAAATAATTTACATATTGAAATTGTAATACATGTATTTCATAATTCACATAATTTAAAGATGGTTTTCCGATAAATTTTAAAGTATTCATACTAAGAGTAACCGAAATATACTACTACGTACTTAATAATTAAATTGCACAATTTAGAAAGATATGGGGCTGATTTACTTTAATTAAAACTGTAAAATTTTATTATATACTACGTACGTAAAGGTTTACAAGAATTTGTATTTCCTATATAACCTTTGACTTATTAAAAATTAATTTTTTCTATTTATGTCAAGATCTTTAACATAGTATATTTCTTATCAATGGTTTTTTCTTCTTTTTAAGCTGAGGAAACTATTGATTTTTTTAGCCAATAGGATAACCTTAATGTTTTGTCAATTCTTTTCTTTATGTGCTCATTTAAGCTTCAGCGCGACCAAATGATCAAATAAAATACTCCAGTATTTATTGTACCTTAAATAAGGTATAACCTATAACCGGTTAGTGGTTACACATAGTAAAGTATTGTCAGTATGATGTCATTGGTTTTCAATTCGTATTGTTACAATTACAAGTTAGCTAGTAATCTTCTACGTAGCAATTCCATTAAAGAAACATGTAGGGATCGGGGGACTGTATTAGGATCAGATCGAGTATACTTTCTCCGTTTGGAAATATTTGCAACGTTGGTCACTTTCACACATGCCAACGTACAATTTTAATCGTTAATAAGCAAAAAATTTCAAAAGTTGATATTTTGAAAATATATATATCCATTGAGACGAATCTAACAAGATTACACATGACTATGTTTTATTTGGCATATAAATCACCAACAATAATCAAAGTAAATGCGTGAATAGTGTAAAATGCATGTCCAAATATTAGGAGTATTTCTGAGCAAAGAAAGTATTACTTACACATACTTAAGGCGTGTTTACATGGATTATAATAACAGGTAAACTAAAGAGATCGACAACTAAGTATGAAGTTGTCAGCAACAACAATATGGGTTCTTCGTTGGTGCATGATGATGGTTGTTGTGGTTGCGGTTGCGGTTGTGCCGCCATCACTAGCAGTAGTAGAAGATGAAGAAATGATTGAAAACCTAAGATACACCACTGTAACATTTGAACTAACAAATGGTGTTACAGGCTACTCAAGTTTCATGACAAGGTTGCGCAACGCTGTGGAAGCCCCAACTAGAGCGTGCGGGTTACAATCAACACGAAGCACACCATTACCAGGAGCCGAATACATTCACGTAGACCTCAAAATCAGCAACACACAATGGGTTACTTTAGGAATAGATGTCAAAGACTTCTATGTTTGGGGTTATCAAGACAATGTTAGATATAACGGCAATTATAGAGCAAGTTTCCTTAATGATGCCCCTCAAGCTGCTAAGAACAACCTTTTCCGTGGTTCAACAATTAGAACCACTAGGTTCGGAGGTAATGTTAGAATAATGTCTTGAATTGGTCAAACCCCTTACTGCAACGCCCCACCACGGGGATCTCGTTGCCCGATCAGCGAGTCGGGGTTCATGGGTGACGCCCCTGGTTTTGTGGTTCCGGGGTAACGCCCCCGAACTTACGGTATTTGTTATTTTGGACTAGAGTTATCTGTTTTGGACCTATTTTCTGGATTTTTTCGCATCTGTCACTACAAGAAATTGTTAACGACGGGAAATTCCGTCGCTAAAGGCCAATAATCGTTGATTAACGACGAGATTTCCTGATGCGGACCCGTTATAAAAAGGGACCGTCGTAAATAGAAAATCCCGTCGTTAACCCGTCATAAAAGACATTTACGACGGTTGTTCCCGTCTTTGTTGGGTTGTTAGCCCCATCACAAAAGGCTTTTGCGACGAGATTTTCGACCGTCGTTATTAGGTTGTCATAAAAGATACAAATTCTTGTAGTGTGTCTAGAAAGTACTATACAAAATATCAAGGTCATAACCTAGTCGTATTCTGTAATACGAACCAAATTAAATCTCTGCGTTCTTTATTTACATTATTTAATATTTTTTTACATCCGTTATAACAGGTAACTATAACAGTCTTGAGCCGGCTGCCGGTATTACTCGAAGGAACCTTGTGCTAGGAATTCAGAACCTTGACGGGGCTATAAGAACGGTGTACGGGAAACAAGAAAGTGAGTTAAACAAGGGTAATGCAGAGGCTAGGTTTTTCCTTATTGCCATCCAAATGGTTGCGGAGGCAGCACGTTTTAAATTCATGGAGGAGGCCATTGTTAGGAATGATAACACACCAGATTTCAAAAGGAAAATGGTGGCTTTCCAGAATGATTGGGATCCGATCTCAACTGCGATTCATAGAGCAGAAGCCGCAACACCAAAATGTACTAGGATTACGCCAACCCTCGTAATCAGTAACACTGAGTACAGACAAGAGGTGAATACAGTTGCTGAAATAAAAGATGATATGGGTCTTCTTAAGTATAGATCCACTACTTTTAGTAGTATTGCAATCTCTATTCTGGATGATGAATTCATGGAATCTTCACTGATCTGATCTGTACAGATCAGATTGCCTCGCTGGCATACTTGGCTTATGGTTGTACGTAGTACGAGTGTATTTCATAATAAGTAACCATCTGTTACAAAATAATACGAAGTAATAATCTATGATCTTGTGTATTGGGCTCTAGTCGCCGGCTGTAAAGACATGCATGTAATCTACAACTAAATGAATAAATCTTTCTGTTTTATCAAACCTCTTGTATAAGAGAAATTGCTTAAACTAGTACTATTATTGTTGTGGGTCCGATCAGCCCACTTTTATGTATAAGTAATGTTAATATTTGTGCTTAATTTTTTTATACTCTGTAAGTGGAAGTCCAATTCTCCATTTATT

mRNA sequence

CTATTTAAGGAAGATAGCAAAACATATATTGGGGGTATCCTTTCACTAAATTATCACATATCGATCGTTATTCTTTATACTAGTCATTTTATACAACGCCTCAACAATTCGGTAAACTAAAGAGATCGACAACTAAGTATGAAGTTGTCAGCAACAACAATATGGGTTCTTCGTTGGTGCATGATGATGGTTGTTGTGGTTGCGGTTGCGGTTGTGCCGCCATCACTAGCAGTAGTAGAAGATGAAGAAATGATTGAAAACCTAAGATACACCACTGTAACATTTGAACTAACAAATGGTGTTACAGGCTACTCAAGTTTCATGACAAGGTTGCGCAACGCTGTGGAAGCCCCAACTAGAGCGTGCGGGTTACAATCAACACGAAGCACACCATTACCAGGAGCCGAATACATTCACGTAGACCTCAAAATCAGCAACACACAATGGGTTACTTTAGGAATAGATGTCAAAGACTTCTATGTTTGGGGTTATCAAGACAATGTTAGATATAACGGCAATTATAGAGCAAGTTTCCTTAATGATGCCCCTCAAGCTGCTAAGAACAACCTTTTCCGTGGTTCAACAATTAGAACCACTAGGTTCGGAGGTAACTATAACAGTCTTGAGCCGGCTGCCGGTATTACTCGAAGGAACCTTGTGCTAGGAATTCAGAACCTTGACGGGGCTATAAGAACGGTGTACGGGAAACAAGAAAGTGAGTTAAACAAGGGTAATGCAGAGGCTAGGTTTTTCCTTATTGCCATCCAAATGGTTGCGGAGGCAGCACGTTTTAAATTCATGGAGGAGGCCATTGTTAGGAATGATAACACACCAGATTTCAAAAGGAAAATGGTGGCTTTCCAGAATGATTGGGATCCGATCTCAACTGCGATTCATAGAGCAGAAGCCGCAACACCAAAATGTACTAGGATTACGCCAACCCTCGTAATCAGTAACACTGAGTACAGACAAGAGGTGAATACAGTTGCTGAAATAAAAGATGATATGGGTCTTCTTAAGTATAGATCCACTACTTTTAGTAGTATTGCAATCTCTATTCTGGATGATGAATTCATGGAATCTTCACTGATCTGATCTGTACAGATCAGATTGCCTCGCTGGCATACTTGGCTTATGGTTGTACGTAGTACGAGTGTATTTCATAATAAGTAACCATCTGTTACAAAATAATACGAAGTAATAATCTATGATCTTGTGTATTGGGCTCTAGTCGCCGGCTGTAAAGACATGCATGTAATCTACAACTAAATGAATAAATCTTTCTGTTTTATCAAACCTCTTGTATAAGAGAAATTGCTTAAACTAGTACTATTATTGTTGTGGGTCCGATCAGCCCACTTTTATGTATAAGTAATGTTAATATTTGTGCTTAATTTTTTTATACTCTGTAAGTGGAAGTCCAATTCTCCATTTATT

Coding sequence (CDS)

ATGAAGTTGTCAGCAACAACAATATGGGTTCTTCGTTGGTGCATGATGATGGTTGTTGTGGTTGCGGTTGCGGTTGTGCCGCCATCACTAGCAGTAGTAGAAGATGAAGAAATGATTGAAAACCTAAGATACACCACTGTAACATTTGAACTAACAAATGGTGTTACAGGCTACTCAAGTTTCATGACAAGGTTGCGCAACGCTGTGGAAGCCCCAACTAGAGCGTGCGGGTTACAATCAACACGAAGCACACCATTACCAGGAGCCGAATACATTCACGTAGACCTCAAAATCAGCAACACACAATGGGTTACTTTAGGAATAGATGTCAAAGACTTCTATGTTTGGGGTTATCAAGACAATGTTAGATATAACGGCAATTATAGAGCAAGTTTCCTTAATGATGCCCCTCAAGCTGCTAAGAACAACCTTTTCCGTGGTTCAACAATTAGAACCACTAGGTTCGGAGGTAACTATAACAGTCTTGAGCCGGCTGCCGGTATTACTCGAAGGAACCTTGTGCTAGGAATTCAGAACCTTGACGGGGCTATAAGAACGGTGTACGGGAAACAAGAAAGTGAGTTAAACAAGGGTAATGCAGAGGCTAGGTTTTTCCTTATTGCCATCCAAATGGTTGCGGAGGCAGCACGTTTTAAATTCATGGAGGAGGCCATTGTTAGGAATGATAACACACCAGATTTCAAAAGGAAAATGGTGGCTTTCCAGAATGATTGGGATCCGATCTCAACTGCGATTCATAGAGCAGAAGCCGCAACACCAAAATGTACTAGGATTACGCCAACCCTCGTAATCAGTAACACTGAGTACAGACAAGAGGTGAATACAGTTGCTGAAATAAAAGATGATATGGGTCTTCTTAAGTATAGATCCACTACTTTTAGTAGTATTGCAATCTCTATTCTGGATGATGAATTCATGGAATCTTCACTGATCTGA

Protein sequence

MKLSATTIWVLRWCMMMVVVVAVAVVPPSLAVVEDEEMIENLRYTTVTFELTNGVTGYSSFMTRLRNAVEAPTRACGLQSTRSTPLPGAEYIHVDLKISNTQWVTLGIDVKDFYVWGYQDNVRYNGNYRASFLNDAPQAAKNNLFRGSTIRTTRFGGNYNSLEPAAGITRRNLVLGIQNLDGAIRTVYGKQESELNKGNAEARFFLIAIQMVAEAARFKFMEEAIVRNDNTPDFKRKMVAFQNDWDPISTAIHRAEAATPKCTRITPTLVISNTEYRQEVNTVAEIKDDMGLLKYRSTTFSSIAISILDDEFMESSLI

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo21771.1	Spo21771.1	mRNA

Homology

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BLAST of Spo21771.1 vs. NCBI nr
Match: gi|18149181|dbj|BAB83507.1| (ribosome-inactivating protein [Spinacia oleracea])

HSP 1 Score: 610.5 bits (1573), Expect = 1.600e-171
Identity = 314/319 (98.43%), Postives = 316/319 (99.06%), Query Frame = 1


		  
Query: 1   MKLSATTIWVLRWCMMMVVVVAVAVVPPSLAVVEDEEMIENLRYTTVTFELTNGVTGYSS 60
           MKLSATTIWVLRWCMMMVVVVAVAVVPPSLAVVEDEEMIENLRYTTVTFELTNGVTGYSS
Sbjct: 1   MKLSATTIWVLRWCMMMVVVVAVAVVPPSLAVVEDEEMIENLRYTTVTFELTNGVTGYSS 60

Query: 61  FMTRLRNAVEAPTRACGLQSTRSTPLPGAEYIHVDLKISNTQWVTLGIDVKDFYVWGYQD 120
           FMTRLRNAVEAPTRACGLQSTRSTPLPGAEYIHVDLKISNTQWVTLGIDVKDFYVWGYQD
Sbjct: 61  FMTRLRNAVEAPTRACGLQSTRSTPLPGAEYIHVDLKISNTQWVTLGIDVKDFYVWGYQD 120

Query: 121 NVRYNGNYRASFLNDAPQAAKNNLFRGSTIRTTRFGGNYNSLEPAAGITRRNLVLGIQNL 180
           NVRYNGNYRASFLNDAPQAAKNNLFRGSTIRTTRFGGNYNSLEPAAGITRRNLVLGIQNL
Sbjct: 121 NVRYNGNYRASFLNDAPQAAKNNLFRGSTIRTTRFGGNYNSLEPAAGITRRNLVLGIQNL 180

Query: 181 DGAIRTVYGKQESELNKGNAEARFFLIAIQMVAEAARFKFMEEAIVRNDNTPDFKRKMVA 240
           DGAIRTVYGKQESELNKGNAEARFFLIAIQMVAEAARFKFMEEAIVRNDNTPDFKRKMVA
Sbjct: 181 DGAIRTVYGKQESELNKGNAEARFFLIAIQMVAEAARFKFMEEAIVRNDNTPDFKRKMVA 240

Query: 241 FQNDWDPISTAIHRAEAATPKCTRITPTLVISNTEYRQEVNTVAEIKDDMGLLKYRSTTF 300
           FQNDWDPISTAIHRAEAATPKCTRITPTLVISNTEYRQEVNTVAEIKDDMGLLKYRSTT 
Sbjct: 241 FQNDWDPISTAIHRAEAATPKCTRITPTLVISNTEYRQEVNTVAEIKDDMGLLKYRSTTL 300

Query: 301 SSIAISIL-DDEFMESSLI 319
           SSIA SIL DDEF+ES+LI
Sbjct: 301 SSIASSILDDDEFLESALI 319

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BLAST of Spo21771.1 vs. NCBI nr
Match: gi|99646716|emb|CAK22417.1| (ribosome-inactivating protein [Beta vulgaris])

HSP 1 Score: 415.6 bits (1067), Expect = 7.700e-113
Identity = 222/327 (67.89%), Postives = 254/327 (77.68%), Query Frame = 1


		  
Query: 1   MKLSATTIWVLRWCMMMVVVVAVAVVPPSLAVVEDEEMIENLRYTTVTFELTNGVT---G 60
           MK      W+ +WC++MVVV+    VP +    EDE + + L YTTVTFEL  GV    G
Sbjct: 1   MKALTAARWI-QWCIIMVVVI----VPITATTTEDEAVGKKLSYTTVTFELKYGVEPLKG 60

Query: 61  YSSFMTRLRNAVEAPTRACGLQSTRSTPLPGAEYIHVDLKISNTQWVTLGIDVKDFYVWG 120
           YSSF+TRLRNAVEAPTRACGLQ TR  PL G +Y+ VDLK SNTQWVTLGID KD YVW 
Sbjct: 61  YSSFLTRLRNAVEAPTRACGLQFTRKVPLTGKQYVLVDLKFSNTQWVTLGIDAKDLYVWA 120

Query: 121 YQDNVRYNGNYRASFLNDAPQAAKNNLFRGSTIRTTRFGGNYNSLEPAAGITRRNLVLGI 180
           YQDNV+YNG +RA+FL DAPQ+AKN+LF GST RTTRFGGNYNSLEPAAG+TR N+VLG 
Sbjct: 121 YQDNVKYNGKFRANFLGDAPQSAKNSLFPGSTKRTTRFGGNYNSLEPAAGVTRNNIVLGT 180

Query: 181 QNLDGAIRTVYGKQESELNKGNAEARFFLIAIQMVAEAARFKFMEEAIVRNDNTPDFKRK 240
           QNLDGAI+ VYGKQE  LN+G  EA+FFLIAIQMVAEAARFKFME+ IV+ D T  FK K
Sbjct: 181 QNLDGAIKRVYGKQEGVLNQGKDEAKFFLIAIQMVAEAARFKFMEQGIVKGDKTTAFKAK 240

Query: 241 MVAFQNDWDPISTAIHRAEAATPKCTRITPTLVISNTEYRQEVNTVAEIKDDMGLLKYRS 300
           MVAFQNDWDPIS AIHRAEAA  KCT I+PTLVISNT YRQEVN V  +K+DMGLLKY+S
Sbjct: 241 MVAFQNDWDPISQAIHRAEAAARKCTTISPTLVISNTGYRQEVNRVDAVKNDMGLLKYKS 300

Query: 301 TTFSSIAISILDD------EFMESSLI 319
              SS+  SIL D      EF+ES+++
Sbjct: 301 IG-SSVVNSILADDGGSSLEFLESAVL 321

[Close]

BLAST of Spo21771.1 vs. NCBI nr
Match: gi|731354502|ref|XP_010688631.1| (PREDICTED: ribosome-inactivating protein PD-L3/PD-L4-like isoform X1 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 411.4 bits (1056), Expect = 1.500e-111
Identity = 221/327 (67.58%), Postives = 252/327 (77.06%), Query Frame = 1


		  
Query: 1   MKLSATTIWVLRWCMMMVVVVAVAVVPPSLAVVEDEEMIENLRYTTVTFELTNGVT---G 60
           MK      W+ +WC++MVVV+    VP +    EDE + + L YTTVTFEL  GV    G
Sbjct: 1   MKALTAARWI-QWCIIMVVVI----VPITATTTEDEAVGKKLSYTTVTFELKYGVEPLKG 60

Query: 61  YSSFMTRLRNAVEAPTRACGLQSTRSTPLPGAEYIHVDLKISNTQWVTLGIDVKDFYVWG 120
           YSSF+TRLRNAVEAPTRACGLQ TR  PL G +Y+ VDLK SNTQWVTLGID KD YVW 
Sbjct: 61  YSSFLTRLRNAVEAPTRACGLQFTRKVPLTGKQYVLVDLKFSNTQWVTLGIDAKDLYVWA 120

Query: 121 YQDNVRYNGNYRASFLNDAPQAAKNNLFRGSTIRTTRFGGNYNSLEPAAGITRRNLVLGI 180
           YQDNV YNG +RA+FL DAPQ+AKN+LF GST RTTRFGGNYNSLEPAAG+TR N+VLG 
Sbjct: 121 YQDNVIYNGKFRANFLGDAPQSAKNSLFPGSTKRTTRFGGNYNSLEPAAGVTRNNIVLGT 180

Query: 181 QNLDGAIRTVYGKQESELNKGNAEARFFLIAIQMVAEAARFKFMEEAIVRNDNTPDFKRK 240
           QNLDGAI+ VYGKQE  LN+G  EA+FFLIAIQMVAEAARFKFME+ IV+ D T  FK K
Sbjct: 181 QNLDGAIKRVYGKQEGVLNQGKDEAKFFLIAIQMVAEAARFKFMEQGIVKGDKTTAFKAK 240

Query: 241 MVAFQNDWDPISTAIHRAEAATPKCTRITPTLVISNTEYRQEVNTVAEIKDDMGLLKYRS 300
           MVAFQNDWDPIS AIHRAEAA  KCT I+PTLVISN  YRQEVN V  +K+DMGLLKY+S
Sbjct: 241 MVAFQNDWDPISQAIHRAEAAARKCTTISPTLVISNIGYRQEVNRVDAVKNDMGLLKYKS 300

Query: 301 TTFSSIAISILDD------EFMESSLI 319
              SS+  SIL D      EF+ES+++
Sbjct: 301 IG-SSVVNSILADDGGSSLEFLESAVL 321

[Close]

BLAST of Spo21771.1 vs. NCBI nr
Match: gi|1021023582|gb|KZM81370.1| (hypothetical protein DCAR_028983 [Daucus carota subsp. sativus])

HSP 1 Score: 355.9 bits (912), Expect = 7.300e-95
Identity = 193/296 (65.20%), Postives = 230/296 (77.70%), Query Frame = 1


		  
Query: 1   MKLSATTIWVLRWCMMMVVVVAVAVVPPSLAVVEDEEMIEN-LRYTTVTFELTNGVTGYS 60
           MKL+  TI V+ WCMM VV         S AVVE EE+ E  L Y  VTFEL  GV  YS
Sbjct: 4   MKLA--TICVI-WCMMAVVA--------SSAVVEYEEIGEKPLGY--VTFELARGVQSYS 63

Query: 61  SFMTRLRNAVEAPTRACGLQSTRSTPLPGAEYIHVDLKISNTQWVTLGIDVKDFYVWGYQ 120
           SF+TRLRNAVEAPTRACGLQSTR TPLPG EY + +L +SNT+W+TLGI+ K+ YVW YQ
Sbjct: 64  SFLTRLRNAVEAPTRACGLQSTRITPLPGTEYGYANLLLSNTEWITLGIETKNLYVWAYQ 123

Query: 121 DNVRYNGNYRASFLNDAPQAAKNNLFRGSTIRTTRFGGNYNSLEPAAGITRRNLVLGIQN 180
           DNV+Y+G YRA+FL++AP  AK+NLF GSTIR +R   +Y  LE AA  +R +L+LGI+ 
Sbjct: 124 DNVKYDGKYRANFLSNAPTEAKSNLFSGSTIRISRLPSDYTDLERAARASRYDLILGIEK 183

Query: 181 LDGAIRTVYGKQESELNKGNAEARFFLIAIQMVAEAARFKFMEEAIVRNDNTPDFKRKMV 240
           L GA+R VYG+QE++LN+G  EA+F L+AIQMVAEAARFKFMEE IVRNDNTP  K KMV
Sbjct: 184 LRGAMRGVYGRQENQLNQGKEEAKFLLLAIQMVAEAARFKFMEEGIVRNDNTPYMKLKMV 243

Query: 241 AFQNDWDPISTAIHRAEAATPKCTRITPTLVISNTEYRQEVNTVAEIKDDMGLLKY 296
           AF+NDW+ IS AIH+AE ATPKC +ITPTLVISN  YRQEVN VAEI +D+G+LKY
Sbjct: 244 AFENDWNKISKAIHQAEGATPKCIKITPTLVISNIGYRQEVNEVAEIINDIGILKY 286

[Close]

BLAST of Spo21771.1 vs. NCBI nr
Match: gi|870850460|gb|KMT02560.1| (hypothetical protein BVRB_9g202470 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 311.2 bits (796), Expect = 2.100e-81
Identity = 163/239 (68.20%), Postives = 185/239 (77.41%), Query Frame = 1


		  
Query: 1   MKLSATTIWVLRWCMMMVVVVAVAVVPPSLAVVEDEEMIENLRYTTVTFELTNGVT---G 60
           MK      W+ +WC++MVVV+    VP +    EDE + + L YTTVTFEL  GV    G
Sbjct: 1   MKALTAARWI-QWCIIMVVVI----VPITATTTEDEAVGKKLSYTTVTFELKYGVEPLKG 60

Query: 61  YSSFMTRLRNAVEAPTRACGLQSTRSTPLPGAEYIHVDLKISNTQWVTLGIDVKDFYVWG 120
           YSSF+TRLRNAVEAPTRACGLQ TR  PL G +Y+ VDLK SNTQWVTLGID KD YVW 
Sbjct: 61  YSSFLTRLRNAVEAPTRACGLQFTRKVPLTGKQYVLVDLKFSNTQWVTLGIDAKDLYVWA 120

Query: 121 YQDNVRYNGNYRASFLNDAPQAAKNNLFRGSTIRTTRFGGNYNSLEPAAGITRRNLVLGI 180
           YQDNV YNG +RA+FL DAPQ+AKN+LF GST RTTRFGGNYNSLEPAAG+TR N+VLG 
Sbjct: 121 YQDNVIYNGKFRANFLGDAPQSAKNSLFPGSTKRTTRFGGNYNSLEPAAGVTRNNIVLGT 180

Query: 181 QNLDGAIRTVYGKQESELNKGNAEARFFLIAIQMVAEAARFKFMEEAIVRNDNTPDFKR 237
           QNLDGAI+ VYGKQE  LN+G  EA+FFLIAIQMVAEAARFKFME+ IV+ D T  F R
Sbjct: 181 QNLDGAIKRVYGKQEGVLNQGKDEAKFFLIAIQMVAEAARFKFMEQGIVKGDKTTAFVR 234

[Close]

BLAST of Spo21771.1 vs. UniProtKB/TrEMBL
Match: Q8VWY2_SPIOL (rRNA N-glycosidase OS=Spinacia oleracea GN=BP31/SoRIP1 PE=2 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 1.100e-171
Identity = 314/319 (98.43%), Postives = 316/319 (99.06%), Query Frame = 1


		  
Query: 1   MKLSATTIWVLRWCMMMVVVVAVAVVPPSLAVVEDEEMIENLRYTTVTFELTNGVTGYSS 60
           MKLSATTIWVLRWCMMMVVVVAVAVVPPSLAVVEDEEMIENLRYTTVTFELTNGVTGYSS
Sbjct: 1   MKLSATTIWVLRWCMMMVVVVAVAVVPPSLAVVEDEEMIENLRYTTVTFELTNGVTGYSS 60

Query: 61  FMTRLRNAVEAPTRACGLQSTRSTPLPGAEYIHVDLKISNTQWVTLGIDVKDFYVWGYQD 120
           FMTRLRNAVEAPTRACGLQSTRSTPLPGAEYIHVDLKISNTQWVTLGIDVKDFYVWGYQD
Sbjct: 61  FMTRLRNAVEAPTRACGLQSTRSTPLPGAEYIHVDLKISNTQWVTLGIDVKDFYVWGYQD 120

Query: 121 NVRYNGNYRASFLNDAPQAAKNNLFRGSTIRTTRFGGNYNSLEPAAGITRRNLVLGIQNL 180
           NVRYNGNYRASFLNDAPQAAKNNLFRGSTIRTTRFGGNYNSLEPAAGITRRNLVLGIQNL
Sbjct: 121 NVRYNGNYRASFLNDAPQAAKNNLFRGSTIRTTRFGGNYNSLEPAAGITRRNLVLGIQNL 180

Query: 181 DGAIRTVYGKQESELNKGNAEARFFLIAIQMVAEAARFKFMEEAIVRNDNTPDFKRKMVA 240
           DGAIRTVYGKQESELNKGNAEARFFLIAIQMVAEAARFKFMEEAIVRNDNTPDFKRKMVA
Sbjct: 181 DGAIRTVYGKQESELNKGNAEARFFLIAIQMVAEAARFKFMEEAIVRNDNTPDFKRKMVA 240

Query: 241 FQNDWDPISTAIHRAEAATPKCTRITPTLVISNTEYRQEVNTVAEIKDDMGLLKYRSTTF 300
           FQNDWDPISTAIHRAEAATPKCTRITPTLVISNTEYRQEVNTVAEIKDDMGLLKYRSTT 
Sbjct: 241 FQNDWDPISTAIHRAEAATPKCTRITPTLVISNTEYRQEVNTVAEIKDDMGLLKYRSTTL 300

Query: 301 SSIAISIL-DDEFMESSLI 319
           SSIA SIL DDEF+ES+LI
Sbjct: 301 SSIASSILDDDEFLESALI 319

[Close]

BLAST of Spo21771.1 vs. UniProtKB/TrEMBL
Match: Q1H8N0_BETVU (rRNA N-glycosidase OS=Beta vulgaris GN=rip-r1 PE=2 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 5.400e-113
Identity = 222/327 (67.89%), Postives = 254/327 (77.68%), Query Frame = 1


		  
Query: 1   MKLSATTIWVLRWCMMMVVVVAVAVVPPSLAVVEDEEMIENLRYTTVTFELTNGVT---G 60
           MK      W+ +WC++MVVV+    VP +    EDE + + L YTTVTFEL  GV    G
Sbjct: 1   MKALTAARWI-QWCIIMVVVI----VPITATTTEDEAVGKKLSYTTVTFELKYGVEPLKG 60

Query: 61  YSSFMTRLRNAVEAPTRACGLQSTRSTPLPGAEYIHVDLKISNTQWVTLGIDVKDFYVWG 120
           YSSF+TRLRNAVEAPTRACGLQ TR  PL G +Y+ VDLK SNTQWVTLGID KD YVW 
Sbjct: 61  YSSFLTRLRNAVEAPTRACGLQFTRKVPLTGKQYVLVDLKFSNTQWVTLGIDAKDLYVWA 120

Query: 121 YQDNVRYNGNYRASFLNDAPQAAKNNLFRGSTIRTTRFGGNYNSLEPAAGITRRNLVLGI 180
           YQDNV+YNG +RA+FL DAPQ+AKN+LF GST RTTRFGGNYNSLEPAAG+TR N+VLG 
Sbjct: 121 YQDNVKYNGKFRANFLGDAPQSAKNSLFPGSTKRTTRFGGNYNSLEPAAGVTRNNIVLGT 180

Query: 181 QNLDGAIRTVYGKQESELNKGNAEARFFLIAIQMVAEAARFKFMEEAIVRNDNTPDFKRK 240
           QNLDGAI+ VYGKQE  LN+G  EA+FFLIAIQMVAEAARFKFME+ IV+ D T  FK K
Sbjct: 181 QNLDGAIKRVYGKQEGVLNQGKDEAKFFLIAIQMVAEAARFKFMEQGIVKGDKTTAFKAK 240

Query: 241 MVAFQNDWDPISTAIHRAEAATPKCTRITPTLVISNTEYRQEVNTVAEIKDDMGLLKYRS 300
           MVAFQNDWDPIS AIHRAEAA  KCT I+PTLVISNT YRQEVN V  +K+DMGLLKY+S
Sbjct: 241 MVAFQNDWDPISQAIHRAEAAARKCTTISPTLVISNTGYRQEVNRVDAVKNDMGLLKYKS 300

Query: 301 TTFSSIAISILDD------EFMESSLI 319
              SS+  SIL D      EF+ES+++
Sbjct: 301 IG-SSVVNSILADDGGSSLEFLESAVL 321

[Close]

BLAST of Spo21771.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BMK1_BETVU (rRNA N-glycosidase OS=Beta vulgaris subsp. vulgaris GN=BVRB_9g202470 PE=3 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 1.400e-81
Identity = 163/239 (68.20%), Postives = 185/239 (77.41%), Query Frame = 1


		  
Query: 1   MKLSATTIWVLRWCMMMVVVVAVAVVPPSLAVVEDEEMIENLRYTTVTFELTNGVT---G 60
           MK      W+ +WC++MVVV+    VP +    EDE + + L YTTVTFEL  GV    G
Sbjct: 1   MKALTAARWI-QWCIIMVVVI----VPITATTTEDEAVGKKLSYTTVTFELKYGVEPLKG 60

Query: 61  YSSFMTRLRNAVEAPTRACGLQSTRSTPLPGAEYIHVDLKISNTQWVTLGIDVKDFYVWG 120
           YSSF+TRLRNAVEAPTRACGLQ TR  PL G +Y+ VDLK SNTQWVTLGID KD YVW 
Sbjct: 61  YSSFLTRLRNAVEAPTRACGLQFTRKVPLTGKQYVLVDLKFSNTQWVTLGIDAKDLYVWA 120

Query: 121 YQDNVRYNGNYRASFLNDAPQAAKNNLFRGSTIRTTRFGGNYNSLEPAAGITRRNLVLGI 180
           YQDNV YNG +RA+FL DAPQ+AKN+LF GST RTTRFGGNYNSLEPAAG+TR N+VLG 
Sbjct: 121 YQDNVIYNGKFRANFLGDAPQSAKNSLFPGSTKRTTRFGGNYNSLEPAAGVTRNNIVLGT 180

Query: 181 QNLDGAIRTVYGKQESELNKGNAEARFFLIAIQMVAEAARFKFMEEAIVRNDNTPDFKR 237
           QNLDGAI+ VYGKQE  LN+G  EA+FFLIAIQMVAEAARFKFME+ IV+ D T  F R
Sbjct: 181 QNLDGAIKRVYGKQEGVLNQGKDEAKFFLIAIQMVAEAARFKFMEQGIVKGDKTTAFVR 234

[Close]

BLAST of Spo21771.1 vs. UniProtKB/TrEMBL
Match: A0A0K9R3P2_SPIOL (rRNA N-glycosidase OS=Spinacia oleracea GN=SOVF_110510 PE=3 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 3.200e-81
Identity = 170/310 (54.84%), Postives = 218/310 (70.32%), Query Frame = 1


		  
Query: 1   MKLSATTIWVLRWCMMMVVVVAVAVVPPSLAVVEDEEMIENL-----RYTTVTFELTNGV 60
           MK+     W+  WC +++ VV +     SL +++ E++   L      +TTVTFEL +G+
Sbjct: 1   MKVFTAAKWIW-WCTVIITVVVML----SLTLIKKEQVGRQLGQQVGAFTTVTFELDDGL 60

Query: 61  TGYSSFMTRLRNAVEAPTRACGLQSTRSTPLPGAEYIHVDLKISNTQWVTLGIDVKDFYV 120
           +GYS F+TRLR+A+EAPT+ACGLQ+T  +P  G EYIHVD+K SNTQWVT+GIDV D YV
Sbjct: 61  SGYSEFLTRLRDALEAPTKACGLQTTVGSPSKGEEYIHVDIKFSNTQWVTIGIDVMDMYV 120

Query: 121 WGYQDNVRYNGNYRASFLNDAPQAAKNNLFRGSTIRTTRFGGNYNSLEPAAGITRRNLVL 180
           WGY+DNV+Y G  RA+F  + P+ AK+NLF  +T RTT F   Y SLE  A + R +L L
Sbjct: 121 WGYKDNVKYKGKSRATFFKNTPKVAKDNLFPDTTPRTTTFLSTYGSLERYAKVGRVDLEL 180

Query: 181 GIQNLDGAIRTVYGKQESELNKGNAEARFFLIAIQMVAEAARFKFMEEAIVRNDNTPDFK 240
            ++NL   I +VYGK E EL+    EA+F LIA+QMVAEA RF+FME+ IV  D++  FK
Sbjct: 181 TLKNLKACIISVYGKPEKELDNA-VEAKFVLIAVQMVAEATRFRFMEKGIVNFDSSKAFK 240

Query: 241 RKMVAFQNDWDPISTAIHRAEAATPKCTRITPTLVISNTEYRQEVNTVAEIKDDMGLLKY 300
            KMVAFQNDWDP S AIH+   A+P CT I+PTLVIS  +YRQEV  V EIKDDMGLLK+
Sbjct: 241 YKMVAFQNDWDPASQAIHK---ASPTCTTISPTLVISTIDYRQEVKEVDEIKDDMGLLKF 300

Query: 301 -RSTTFSSIA 305
            RS T  SI+
Sbjct: 301 KRSNTIQSIS 301

[Close]

BLAST of Spo21771.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BRN0_BETVU (rRNA N-glycosidase OS=Beta vulgaris subsp. vulgaris GN=BVRB_9g202450 PE=3 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 1.500e-78
Identity = 172/298 (57.72%), Postives = 209/298 (70.13%), Query Frame = 1


		  
Query: 1   MKLSATTIWVLRWCMMMVVVVAVAVVPPSLAVVEDEEMIENLRYTTVTFELT--NGVTGY 60
           MK+SA  IW+  WC+MMV+VV      PSLA++ DE   + L Y TV+++L+  + V  Y
Sbjct: 1   MKVSAAAIWMW-WCIMMVMVVM-----PSLAILYDE-CDQQLGYNTVSWDLSLPDPVKCY 60

Query: 61  SSFMTRLRNAVEAPTRACGLQSTRSTPLPGAEYIHVDLKISNTQWVTLGIDVKDFYVWGY 120
             F+TRLRN VEAP R CGL STR  PL G EYI VDLKISN  WVT+GIDV D YVWGY
Sbjct: 61  PKFLTRLRNQVEAPIRICGLPSTRHAPLEGQEYITVDLKISNRNWVTIGIDVIDLYVWGY 120

Query: 121 QDNVRYNGNYRASFLNDAPQAAKNNLFRGSTI-RTTRFGGNYNSLEPAAGITRRNLVLGI 180
           QD  ++NG  R++F+ND PQAA+N LF+ + I RTT F GNY+SLEP A   R  L LG+
Sbjct: 121 QD--KFNGILRSTFVNDVPQAARNTLFQDAAIRRTTNFAGNYDSLEPYARTDRTRLELGV 180

Query: 181 QNLDGAIRTVYGKQESELNKGNAEARFFLIAIQMVAEAARFKFMEEAIVRNDNTPDFKRK 240
             L GAIR+VYGK E+ELN   AEA+FFLI IQMVAEA+RFKFME AIV  DN+ D K K
Sbjct: 181 DKLQGAIRSVYGKPETELNNA-AEAKFFLIVIQMVAEASRFKFMENAIVWKDNSDDTKLK 240

Query: 241 MVAFQNDWDPISTAIHRAEAATPKCTRITPTLVISNTEYRQEVNTVAEIKDDMGLLKY 296
           M AFQNDW  IS A + A   T +CT I P + + N EY+Q V  V +IK+D+GLLKY
Sbjct: 241 MKAFQNDWAKISKAFYNASTYT-RCTTINPPIEVGNVEYKQMVYNVNDIKEDIGLLKY 287

[Close]

BLAST of Spo21771.1 vs. ExPASy Swiss-Prot
Match: RIPP_MIRJA (Antiviral protein MAP OS=Mirabilis jalapa PE=1 SV=2)

HSP 1 Score: 132.9 bits (333), Expect = 6.300e-30
Identity = 100/272 (36.76%), Postives = 142/272 (52.21%), Query Frame = 1


		  
Query: 31  AVVEDEEMIENLRYTTVTFELTNGVTGYSSFMTRLRNAVEAPTRACGLQSTRSTPLPGAE 90
           A+V  +        T  + +L N  T Y SF+T +R  V   T  C +Q    T      
Sbjct: 20  AIVNPQSRAAPTLETIASLDLNNPTT-YLSFITNIRTKVADKTEQCTIQKISKTFTQ--R 79

Query: 91  YIHVDLKISNTQWVTLGIDVKDFYVWGYQDNVRYNGNYRASFLNDAPQAAKNNLFRGST- 150
           Y ++DL +S+TQ +TL ID+ D YV GY D     G  RA F  D  +A  NN F G+T 
Sbjct: 80  YSYIDLIVSSTQKITLAIDMADLYVLGYSDIANNKG--RAFFFKDVTEAVANNFFPGATG 139

Query: 151 ---IRTTRFGGNYNSLEPAAGITRRNLVLGIQNLDGAIRTVYGKQESELNKGNAEARFFL 210
              I+ T F G+Y  LE   G+ + N  LGI  L+ +I  +YGK   ++ K   +A+FFL
Sbjct: 140 TNRIKLT-FTGSYGDLEKNGGLRKDN-PLGIFRLENSIVNIYGKA-GDVKK---QAKFFL 199

Query: 211 IAIQMVAEAARFKFMEEAIVRND-NTPDFKRKMVAFQNDWDPISTAIHRAEAAT---PKC 270
           +AIQMV+EAARFK++ + I             M A +N+W  +STA++ ++ +T    KC
Sbjct: 200 LAIQMVSEAARFKYISDKIPSEKYEEVTVDEYMTALENNWAKLSTAVYNSKPSTTTATKC 259

Query: 271 TRITPTLVISNTEYRQEVNTVAEIKDDMGLLK 295
              T  + IS   ++    TV EIK  MGLLK
Sbjct: 260 QLATSPVTISPWIFK----TVEEIKLVMGLLK 276

[Close]

BLAST of Spo21771.1 vs. ExPASy Swiss-Prot
Match: RIPL2_PHYDI (Ribosome-inactivating protein PD-L3/PD-L4 OS=Phytolacca dioica PE=1 SV=2)

HSP 1 Score: 109.8 bits (273), Expect = 5.700e-23
Identity = 92/269 (34.20%), Postives = 136/269 (50.56%), Query Frame = 1


		  
Query: 46  TVTFELTNG-VTGYSSFMTRLRNAVEAPTRACG----LQSTRSTPLPGAEYIHVDLKISN 105
           T+TF++ N  +  Y++FM  LRN  + PT  C     L  +  TP    +Y+ V L+ ++
Sbjct: 3   TITFDVGNATINKYATFMESLRNEAKDPTLKCYGIPMLPDSNLTP----KYVLVKLQDAS 62

Query: 106 TQWVTLGIDVKDFYVWGYQDNVRYNGNYRASFLNDAPQA----AKNNLFRGSTIRTTR-- 165
           ++ +TL +   + YV GY D   YNG  R    ND         +N L   S  R  +  
Sbjct: 63  SKTITLMLRRNNLYVMGYSD--LYNGKCRYHIFNDISSTESTDVENTLCPNSNSREKKAI 122

Query: 166 -FGGNYNSLEPAAGITRRNLV-LGIQNLDGAIRTVYGKQESELNKGNAEARFFLIAIQMV 225
            +   Y++L+  AG++ R+ V LGIQ L+  I  + G           EA F L+AIQMV
Sbjct: 123 NYNSQYSTLQNKAGVSSRSQVQLGIQILNSDIGKISGVSTFT---DKTEAEFLLVAIQMV 182

Query: 226 AEAARFKFMEEAIVRNDNTP-DFKRKMVAFQNDWDPISTAIHRAEAATPKCTRITPTLVI 285
           +EAARFK++E  +  N N   +   K+++ + +W  IS AIH A     K   +T  L +
Sbjct: 183 SEAARFKYIENQVKTNFNRAFNPNPKVLSLEENWGKISLAIHNA-----KNGALTSPLEL 242

Query: 286 SNT-EYRQEVNTVAEIKDDMGLLKYRSTT 300
            N  + +  V  V EIK DMGLL Y S T
Sbjct: 243 KNADDTKWIVLRVDEIKPDMGLLNYVSGT 257

[Close]

BLAST of Spo21771.1 vs. ExPASy Swiss-Prot
Match: RIPS_PHYAM (Antiviral protein S OS=Phytolacca americana PE=1 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 4.800e-22
Identity = 98/271 (36.16%), Postives = 139/271 (51.29%), Query Frame = 1


		  
Query: 46  TVTFELTNG-VTGYSSFMTRLRNAVEAPTRACG----LQSTRSTPLPGAEYIHVDLKISN 105
           T+TF+  N  +  Y++FM  LRN  + P+  C     L +T ST     +Y+ V L+ ++
Sbjct: 3   TITFDAGNATINKYATFMESLRNEAKDPSLKCYGIPMLPNTNST----IKYLLVKLQGAS 62

Query: 106 TQWVTLGIDVKDFYVWGYQDNVRYNGNYRASFLNDAPQA----AKNNLFRGSTIRTTR-- 165
            + +TL +   + YV GY D   Y+   R    ND         +N L   S  R  +  
Sbjct: 63  LKTITLMLRRNNLYVMGYSDP--YDNKCRYHIFNDIKGTEYSDVENTLCPSSNPRVAKPI 122

Query: 166 -FGGNYNSLEPAAGITRRNLV-LGIQNLDGAIRTVYGKQESELNKGNAEARFFLIAIQMV 225
            + G Y +LE  AG+T RN V LGIQ L   I  + G Q S   K   EA+F L+AIQMV
Sbjct: 123 NYNGLYPTLEKKAGVTSRNEVQLGIQILSSDIGKISG-QGSFTEK--IEAKFLLVAIQMV 182

Query: 226 AEAARFKFMEEAIVRNDNTPDF--KRKMVAFQNDWDPISTAIHRAE-AATPKCTRITPTL 285
           +EAARFK++E  +  N N  DF    K++  + +W  ISTAIH ++  A PK       L
Sbjct: 183 SEAARFKYIENQVKTNFNR-DFSPNDKVLDLEENWGKISTAIHNSKNGALPK------PL 242

Query: 286 VISNTE-YRQEVNTVAEIKDDMGLLKYRSTT 300
            + N +  +  V  V EIK D+GLL Y + T
Sbjct: 243 ELKNADGTKWIVLRVDEIKPDVGLLNYVNGT 257

[Close]

BLAST of Spo21771.1 vs. ExPASy Swiss-Prot
Match: NIGB_SAMNI (Nigrin b OS=Sambucus nigra PE=1 SV=2)

HSP 1 Score: 105.9 bits (263), Expect = 8.200e-22
Identity = 77/226 (34.07%), Postives = 118/226 (52.21%), Query Frame = 1


		  
Query: 39  IENLRYTTVTFELTNGVTG-YSSFMTRLRNAVEAPT-RACGLQSTR--STPLPGAEYIHV 98
           I+ + Y +V+F L    +  Y  F++ LR  V   T    GL   R  S     + ++ V
Sbjct: 23  IQGIDYPSVSFNLDGAKSATYRDFLSNLRKTVATGTYEVNGLPVLRRESEVQVKSRFVLV 82

Query: 99  DLKISNTQWVTLGIDVKDFYVWGYQDNVRYNGNYRASFLNDAPQAAKNNLFRGSTIRTTR 158
            L   N   VTL +DV + YV      V ++GN  + F  DA +  K+NLF G+   T  
Sbjct: 83  PLTNYNGNTVTLAVDVTNLYV------VAFSGNANSYFFKDATEVQKSNLFVGTKQNTLS 142

Query: 159 FGGNYNSLEPAAGITRRNLVLGIQNLDGAIRTVYGKQESELNKGNAEARFFLIAIQMVAE 218
           F GNY++LE AA   R ++ LG   LDGAI ++Y         G++ AR  L+ IQMV+E
Sbjct: 143 FTGNYDNLETAANTRRESIELGPSPLDGAITSLY--------HGDSVARSLLVVIQMVSE 202

Query: 219 AARFKFMEEAIVRN-----DNTPDFKRKMVAFQNDWDPISTAIHRA 256
           AARF+++E+ + R+       TP+    M++ +N+W  +S  I +A
Sbjct: 203 AARFRYIEQEVRRSLQQATSFTPN--ALMLSMENNWSSMSLEIQQA 232

[Close]

BLAST of Spo21771.1 vs. ExPASy Swiss-Prot
Match: RIPA_PHYAM (Antiviral protein alpha OS=Phytolacca americana PE=1 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 3.100e-21
Identity = 97/304 (31.91%), Postives = 144/304 (47.37%), Query Frame = 1


		  
Query: 15  MMMVVVVAVA----VVPPSLAVVEDEEMIENLRYTTVTFELTNG-VTGYSSFMTRLRNAV 74
           MM+VVVV +     + PPS   +            T+TF++ N  +  Y++FM  + N  
Sbjct: 3   MMVVVVVMMLSWLILKPPSTWAIN-----------TITFDVGNATINKYATFMKSIHNQA 62

Query: 75  EAPTRACG----LQSTRSTPLPGAEYIHVDLKISNTQWVTLGIDVKDFYVWGYQDNVRYN 134
           + PT  C     L +T  TP    +Y+ V L+ S+ + +TL +   + YV GY D   YN
Sbjct: 63  KDPTLKCYGIPMLPNTNLTP----KYLLVTLQDSSLKTITLMLKRNNLYVMGYADT--YN 122

Query: 135 GNYRASFLNDAPQAAKNNLFRGSTI--------RTTRFGGNYNSLEPAAGITRRNLVLGI 194
           G  R     D     + N    +          +   +  +Y +LE   G  R  + LGI
Sbjct: 123 GKCRYHIFKDISNTTERNDVMTTLCPNPSSRVGKNINYDSSYPALEKKVGRPRSQVQLGI 182

Query: 195 QNLDGAIRTVYGKQESELNKGNAEARFFLIAIQMVAEAARFKFMEEAIVRNDNTPDF-KR 254
           Q L+  I  +YG  +S   K   EA F L+AIQMV+EAARFK++E  +  N N   +   
Sbjct: 183 QILNSGIGKIYGV-DSFTEK--TEAEFLLVAIQMVSEAARFKYIENQVKTNFNRAFYPNA 242

Query: 255 KMVAFQNDWDPISTAIHRAEAATPKCTRITPTLVISNTE-YRQEVNTVAEIKDDMGLLKY 300
           K++  +  W  ISTAIH A     K   +T  L + N    +  V  V +I+ D+GLLKY
Sbjct: 243 KVLNLEESWGKISTAIHNA-----KNGALTSPLELKNANGSKWIVLRVDDIEPDVGLLKY 281

The following BLAST results are available for this feature:

BLAST of Spo21771.1 vs. NCBI nr
BLAST of Spo21771.1 vs. UniProtKB/TrEMBL
BLAST of Spo21771.1 vs. ExPASy Swiss-Prot
BLAST of Spo21771.1 vs. TAIR (Arabidopsis)

BLAST of Spo21771.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|18149181\|dbj\|BAB83507.1\|	1.6e-171	98.4	ribosome-inactivating protein ...	[more]
gi\|99646716\|emb\|CAK22417.1\|	7.7e-113	67.8	ribosome-inactivating protein ...	[more]
gi\|731354502\|ref\|XP_010688631.1\|	1.5e-111	67.5	PREDICTED: ribosome-inactivati...	[more]
gi\|1021023582\|gb\|KZM81370.1\|	7.3e-95	65.2	hypothetical protein DCAR_0289...	[more]
gi\|870850460\|gb\|KMT02560.1\|	2.1e-81	68.2	hypothetical protein BVRB_9g20...	[more]

BLAST of Spo21771.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
Q8VWY2_SPIOL	1.1e-171	98.4	rRNA N-glycosidase OS=Spinacia...	[more]
Q1H8N0_BETVU	5.4e-113	67.8	rRNA N-glycosidase OS=Beta vul...	[more]
A0A0J8BMK1_BETVU	1.4e-81	68.2	rRNA N-glycosidase OS=Beta vul...	[more]
A0A0K9R3P2_SPIOL	3.2e-81	54.8	rRNA N-glycosidase OS=Spinacia...	[more]
A0A0J8BRN0_BETVU	1.5e-78	57.7	rRNA N-glycosidase OS=Beta vul...	[more]

BLAST of Spo21771.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
RIPP_MIRJA	6.3e-30	36.7	Antiviral protein MAP OS=Mirab...	[more]
RIPL2_PHYDI	5.7e-23	34.2	Ribosome-inactivating protein ...	[more]
RIPS_PHYAM	4.8e-22	36.1	Antiviral protein S OS=Phytola...	[more]
NIGB_SAMNI	8.2e-22	34.0	Nigrin b OS=Sambucus nigra PE=...	[more]
RIPA_PHYAM	3.1e-21	31.9	Antiviral protein alpha OS=Phy...	[more]

BLAST of Spo21771.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 0


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 57..252 score: 1.6
IPR001574	Ribosome-inactivating protein	unknown	SSF56371	Ribosome inactivating proteins (RIP)	coord: 46..299 score: 1.57
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10		coord: 44..216 score: 6.4
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10		coord: 217..297 score: 5.4
IPR017988	Ribosome-inactivating protein conserved site	PROSITE	PS00275	SHIGA_RICIN	coord: 209..225 scor
IPR017989	Ribosome-inactivating protein subgroup	PRINTS	PR00396	SHIGARICIN	coord: 205..225 score: 2.1E-23coord: 236..253 score: 2.1E-23coord: 103..118 score: 2.1E-23coord: 170..184 score: 2.1E-23coord: 56..69 score: 2.1

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0017148	negative regulation of translation
biological_process	GO:0006073	cellular glucan metabolic process
biological_process	GO:0071555	cell wall organization
biological_process	GO:0008152	metabolic process
cellular_component	GO:0048046	apoplast
cellular_component	GO:0005618	cell wall
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function	GO:0016762	xyloglucan:xyloglucosyl transferase activity
molecular_function	GO:0047672	anthranilate N-benzoyltransferase activity
molecular_function	GO:0016597	amino acid binding
molecular_function	GO:0046983	protein dimerization activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo08965	0.74	Barchart \| Table
Spo26705	0.69	Barchart \| Table
Spo19675	0.68	Barchart \| Table
Spo19175	0.67	Barchart \| Table