Spo21908 (gene)

Overview
NameSpo21908
Typegene
OrganismSpinacia oleracea (Spinach)
DescriptionReplication protein A 70 kDa DNA-binding subunit
Locationchr5 : 35359805 .. 35361199 (-)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTTATAATTGATCAAGAGTAAGTAACATATACTACGACAAAATGTATCTACACTTTTATTATAATCAAACTACCTAATGTCCTGTTCTTCTTGATTTTCCCCAGATTGAAGCCAATAATGCTGACATTATGGGATGATTTCGCCTCTAAAGAAGGACAAAAAATCGCTCAGATGATCCAAACAAAACCAATCATCATAGGGATGCGTTTGAAAGTCGTTTCTTATCATGGTAAAATCATGTTATTCCCCTTATACTTGTATGTATTAAAATTTGTGTTATACTTTTTAAAATAGCAACTATAATTAACATATTACATATTTCTTCATATTTTTAAGGCACATCTTTAGCGACAAGATCTTCAAGCACTTTTCTTATAAACATCAGCACTCCAGAAAGTGAAGCTCTACAAGAGTGGTAATAAAATAACTCTATGCACTATAATATACATTTTTTACCTATTTTTACCTATTCCAAAAACTGACACAAGACTACACAGGTTACTACAAAATGAAGACAAAATACAAGTTCTGATTAACAAGAATTCTGAAGCTCCTCTTGCACCATCAATGAAAGAACCTACTGCAGATGACATCATACCAGTCAATTCACTTATAACCGCTGCAAAGGTAAAAGAAAGTCACAAAATGAACACAACTTACAACATTTCAATAACAAAAGTAACCTTTTTAAACTAAAATGTGTACAGAATACATCATCATTTTGGATTAAAGCACATGCTACAGTAACAGATTTCAACCAAAAGTTCTGGTACACTGGTTGCAACACATGTTGGAGGGAAATACATGCGGACCACGGCCAGACATTTAACTGCAGAAACTGCTCGGGACAAGAGCGTATTGTAGAAGCAAGGTATACATATAATTAAGCGTTCATATTATTTTAATAAAACCTTGAATTTTAGTTACTTGTGACTATTTACTGAAACTACAAAATCTTTGTGGGTATATCCATAGATGTCGCTTTAACATAGAACTCAAAGACCCAACTGGTACCATTTCAGCAACTATCTTTGGAAAGATTGCAGAAGAAATTTTTGCACTGGACCCTCAAAAGCTCATGCAATTTAGTGATAAGGTAATAAATATCACTATTTTTATTAATTCTTACATTAAATTCAGGCACTAACTCTAACTCAAATACAGAATGATAAGATTGATACAGCTGCTCTGGGAATATGCTCATCCACTAAAAAATACTACTTCCAACTGAAGCCAAACAGAAACAACGAAAATCAATACATCATCACAAAGGCTTTGGACAAGCTGCAACAAGACCACGCAGAGGATGCACAAAAAAAGACCCCTCTCAAACAAGAAGCTAATGAAAACAGTGTTCACCAACAACAGATAAAAGACAACACAAGCACTTAG

mRNA sequence

ATGCTTATAATTGATCAAGAATTGAAGCCAATAATGCTGACATTATGGGATGATTTCGCCTCTAAAGAAGGACAAAAAATCGCTCAGATGATCCAAACAAAACCAATCATCATAGGGATGCGTTTGAAAGTCGTTTCTTATCATGGCACATCTTTAGCGACAAGATCTTCAAGCACTTTTCTTATAAACATCAGCACTCCAGAAAGTGAAGCTCTACAAGAGTGGTTACTACAAAATGAAGACAAAATACAAGTTCTGATTAACAAGAATTCTGAAGCTCCTCTTGCACCATCAATGAAAGAACCTACTGCAGATGACATCATACCAGTCAATTCACTTATAACCGCTGCAAAGAATACATCATCATTTTGGATTAAAGCACATGCTACAGTAACAGATTTCAACCAAAAGTTCTGGTACACTGGTTGCAACACATGTTGGAGGGAAATACATGCGGACCACGGCCAGACATTTAACTGCAGAAACTGCTCGGGACAAGAGCGTATTGTAGAAGCAAGATGTCGCTTTAACATAGAACTCAAAGACCCAACTGGTACCATTTCAGCAACTATCTTTGGAAAGATTGCAGAAGAAATTTTTGCACTGGACCCTCAAAAGCTCATGCAATTTAGTGATAAGGCACTAACTCTAACTCAAATACAGAATGATAAGATTGATACAGCTGCTCTGGGAATATGCTCATCCACTAAAAAATACTACTTCCAACTGAAGCCAAACAGAAACAACGAAAATCAATACATCATCACAAAGGCTTTGGACAAGCTGCAACAAGACCACGCAGAGGATGCACAAAAAAAGACCCCTCTCAAACAAGAAGCTAATGAAAACAGTGTTCACCAACAACAGATAAAAGACAACACAAGCACTTAG

Coding sequence (CDS)

ATGCTTATAATTGATCAAGAATTGAAGCCAATAATGCTGACATTATGGGATGATTTCGCCTCTAAAGAAGGACAAAAAATCGCTCAGATGATCCAAACAAAACCAATCATCATAGGGATGCGTTTGAAAGTCGTTTCTTATCATGGCACATCTTTAGCGACAAGATCTTCAAGCACTTTTCTTATAAACATCAGCACTCCAGAAAGTGAAGCTCTACAAGAGTGGTTACTACAAAATGAAGACAAAATACAAGTTCTGATTAACAAGAATTCTGAAGCTCCTCTTGCACCATCAATGAAAGAACCTACTGCAGATGACATCATACCAGTCAATTCACTTATAACCGCTGCAAAGAATACATCATCATTTTGGATTAAAGCACATGCTACAGTAACAGATTTCAACCAAAAGTTCTGGTACACTGGTTGCAACACATGTTGGAGGGAAATACATGCGGACCACGGCCAGACATTTAACTGCAGAAACTGCTCGGGACAAGAGCGTATTGTAGAAGCAAGATGTCGCTTTAACATAGAACTCAAAGACCCAACTGGTACCATTTCAGCAACTATCTTTGGAAAGATTGCAGAAGAAATTTTTGCACTGGACCCTCAAAAGCTCATGCAATTTAGTGATAAGGCACTAACTCTAACTCAAATACAGAATGATAAGATTGATACAGCTGCTCTGGGAATATGCTCATCCACTAAAAAATACTACTTCCAACTGAAGCCAAACAGAAACAACGAAAATCAATACATCATCACAAAGGCTTTGGACAAGCTGCAACAAGACCACGCAGAGGATGCACAAAAAAAGACCCCTCTCAAACAAGAAGCTAATGAAAACAGTGTTCACCAACAACAGATAAAAGACAACACAAGCACTTAG

Protein sequence

MLIIDQELKPIMLTLWDDFASKEGQKIAQMIQTKPIIIGMRLKVVSYHGTSLATRSSSTFLINISTPESEALQEWLLQNEDKIQVLINKNSEAPLAPSMKEPTADDIIPVNSLITAAKNTSSFWIKAHATVTDFNQKFWYTGCNTCWREIHADHGQTFNCRNCSGQERIVEARCRFNIELKDPTGTISATIFGKIAEEIFALDPQKLMQFSDKALTLTQIQNDKIDTAALGICSSTKKYYFQLKPNRNNENQYIITKALDKLQQDHAEDAQKKTPLKQEANENSVHQQQIKDNTST
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo21908.1Spo21908.1mRNA


Homology
[Close]
BLAST of Spo21908.1 vs. NCBI nr
Match: gi|902234132|gb|KNA23339.1| (hypothetical protein SOVF_025630 isoform B [Spinacia oleracea])

HSP 1 Score: 566.6 bits (1459), Expect = 2.500e-158
Identity = 288/296 (97.30%), Postives = 288/296 (97.30%), Query Frame = 1 

		  

Query: 1   MLIIDQELKPIMLTLWDDFASKEGQKIAQMIQTKPIIIGMRLKVVSYHGTSLATRSSSTF 60
           MLIIDQELKPIMLTLWDDFASKEGQKIAQMIQTKPIIIGMRLKVVSYHGTSLATRSSSTF
Sbjct: 138 MLIIDQELKPIMLTLWDDFASKEGQKIAQMIQTKPIIIGMRLKVVSYHGTSLATRSSSTF 197

Query: 61  LINISTPESEALQEWLLQNEDKIQVLINKNSEAPLAPSMKEPTADDIIPVNSLITAAKNT 120
           LINISTPESEALQEWLLQNEDKIQVLINKNSEAPLAPSMKEPTADDIIPVNSLITAAKNT
Sbjct: 198 LINISTPESEALQEWLLQNEDKIQVLINKNSEAPLAPSMKEPTADDIIPVNSLITAAKNT 257

Query: 121 SSFWIKAHATVTDFNQKFWYTGCNTCWREIHADHGQTFNCRNCSGQERIVEARCRFNIEL 180
           SSFWIKAHATVTDFNQKFWYTGCNTCWREIHADHGQTFNCRNCSGQERIVEARCRFNIEL
Sbjct: 258 SSFWIKAHATVTDFNQKFWYTGCNTCWREIHADHGQTFNCRNCSGQERIVEARCRFNIEL 317

Query: 181 KDPTGTISATIFGKIAEEIFALDPQKLMQFSDKALTLTQIQNDKIDTAALGICSSTKKYY 240
           KDPTGTISATIFGKIAEEIFALDPQKLMQFSDK        NDKIDTAALGICSSTKKYY
Sbjct: 318 KDPTGTISATIFGKIAEEIFALDPQKLMQFSDK--------NDKIDTAALGICSSTKKYY 377

Query: 241 FQLKPNRNNENQYIITKALDKLQQDHAEDAQKKTPLKQEANENSVHQQQIKDNTST 297
           FQLKPNRNNENQYIITKALDKLQQDHAEDAQKKTPLKQEANENSVHQQQIKDNTST
Sbjct: 378 FQLKPNRNNENQYIITKALDKLQQDHAEDAQKKTPLKQEANENSVHQQQIKDNTST 425
[Close]
BLAST of Spo21908.1 vs. NCBI nr
Match: gi|902234131|gb|KNA23338.1| (hypothetical protein SOVF_025630 isoform A [Spinacia oleracea])

HSP 1 Score: 566.6 bits (1459), Expect = 2.500e-158
Identity = 288/296 (97.30%), Postives = 288/296 (97.30%), Query Frame = 1 

		  

Query: 1   MLIIDQELKPIMLTLWDDFASKEGQKIAQMIQTKPIIIGMRLKVVSYHGTSLATRSSSTF 60
           MLIIDQELKPIMLTLWDDFASKEGQKIAQMIQTKPIIIGMRLKVVSYHGTSLATRSSSTF
Sbjct: 1   MLIIDQELKPIMLTLWDDFASKEGQKIAQMIQTKPIIIGMRLKVVSYHGTSLATRSSSTF 60

Query: 61  LINISTPESEALQEWLLQNEDKIQVLINKNSEAPLAPSMKEPTADDIIPVNSLITAAKNT 120
           LINISTPESEALQEWLLQNEDKIQVLINKNSEAPLAPSMKEPTADDIIPVNSLITAAKNT
Sbjct: 61  LINISTPESEALQEWLLQNEDKIQVLINKNSEAPLAPSMKEPTADDIIPVNSLITAAKNT 120

Query: 121 SSFWIKAHATVTDFNQKFWYTGCNTCWREIHADHGQTFNCRNCSGQERIVEARCRFNIEL 180
           SSFWIKAHATVTDFNQKFWYTGCNTCWREIHADHGQTFNCRNCSGQERIVEARCRFNIEL
Sbjct: 121 SSFWIKAHATVTDFNQKFWYTGCNTCWREIHADHGQTFNCRNCSGQERIVEARCRFNIEL 180

Query: 181 KDPTGTISATIFGKIAEEIFALDPQKLMQFSDKALTLTQIQNDKIDTAALGICSSTKKYY 240
           KDPTGTISATIFGKIAEEIFALDPQKLMQFSDK        NDKIDTAALGICSSTKKYY
Sbjct: 181 KDPTGTISATIFGKIAEEIFALDPQKLMQFSDK--------NDKIDTAALGICSSTKKYY 240

Query: 241 FQLKPNRNNENQYIITKALDKLQQDHAEDAQKKTPLKQEANENSVHQQQIKDNTST 297
           FQLKPNRNNENQYIITKALDKLQQDHAEDAQKKTPLKQEANENSVHQQQIKDNTST
Sbjct: 241 FQLKPNRNNENQYIITKALDKLQQDHAEDAQKKTPLKQEANENSVHQQQIKDNTST 288
[Close]
BLAST of Spo21908.1 vs. NCBI nr
Match: gi|661891754|emb|CDP04679.1| (unnamed protein product [Coffea canephora])

HSP 1 Score: 143.3 bits (360), Expect = 6.900e-31
Identity = 85/272 (31.25%), Postives = 146/272 (53.68%), Query Frame = 1 

		  

Query: 2   LIIDQELKPIMLTLWDDFASKEGQKIAQMIQTKPIIIGMRLKVVSYHGTSLATRSSSTFL 61
           +++++E KP++LTLW++F   +G ++A  I    +IIGM+LK+ +++  SL T+  S  L
Sbjct: 215 IVVNEEKKPMLLTLWNEFEQNQGTQLANSIGNANVIIGMKLKITTFNYLSLTTKPGSGLL 274

Query: 62  INISTPESEALQEWLLQNEDKIQVLINKNSEAPLAPSMKEPTADDIIPVNSLITAAKNTS 121
           IN ST E+ AL+EW   N+++I  LI + +    +  +  P A+DII V + +   K+  
Sbjct: 275 INPSTSEANALKEWYNANKEEIAELIQQIAYKDSSKLLPPPIANDIISVANALNTLKDVK 334

Query: 122 SFWIKAHATVTDFNQKFWYTGCNTCWREIHADHGQTFNCRNCSGQERIVEARCRFNIELK 181
           + WI    +++   QKFW+  C  C + I+ D G    C +C    R+V AR R  I + 
Sbjct: 335 TAWITGKISLSPGQQKFWFEACENCQKTINVDVGWVMRCPSCKEDSRVV-ARTRVGIAVD 394

Query: 182 DPTGTISATIFGKIAEEIFALDPQKLMQFSDKALTLTQIQNDKIDTAALGICSSTKKY-- 241
           D TG+I+  IFG        LD +KL+ F+  AL   +   ++++ +A  + S+ +K+  
Sbjct: 395 DGTGSINTVIFG--------LDAEKLIPFT--ALQFWEAHTEELNFSA-ELASALRKHAI 454

Query: 242 -----YFQLKPNRNNENQYIITKALDKLQQDH 267
                Y++       E +Y I KA    +  H
Sbjct: 455 VCFIRYYESDYQGRKEAKYNIVKAYTTEESAH 474
[Close]
BLAST of Spo21908.1 vs. NCBI nr
Match: gi|1009110771|ref|XP_015897487.1| (PREDICTED: replication protein A 70 kDa DNA-binding subunit B-like [Ziziphus jujuba])

HSP 1 Score: 139.0 bits (349), Expect = 1.300e-29
Identity = 76/214 (35.51%), Postives = 113/214 (52.80%), Query Frame = 1 

		  

Query: 11  IMLTLWDDFASKEGQKIAQMIQTKPIIIGMRLKVVSYHGTSLATRSSSTFLINISTPESE 70
           +MLT+W+ F + E  +I  ++  KPIIIG RLKV SY+G SL+T+SSS+F+I +  P++ 
Sbjct: 4   LMLTMWNQFVNNECTQILNIMNRKPIIIGCRLKVSSYNGISLSTKSSSSFVIELEIPQAA 63

Query: 71  ALQEWLLQNEDKIQVLINKNSEAPLAPSMKEPTADDIIPVNSLITAAKNTSSFWIKAHAT 130
           AL  W   N +K   +I              P+A+DII +  +        SFWI  H  
Sbjct: 64  ALSIWYNANAEKAMDIICGRIGLETKSLSTLPSAEDIITIKMIDNQLNTRRSFWINGHIK 123

Query: 131 VTDFNQKFWYTGCNTCWREIHADHGQTFNCRNCSGQERIVEARCRFNIELKDPTGTISAT 190
           VT+  Q FWY  C  C +    +  Q FNC  C   +     RCR  ++L D + +++AT
Sbjct: 124 VTNLIQHFWYLSCEKCTKATGYEFEQRFNCLYCRHNQVKAIPRCRVIVDLIDESSSLNAT 183

Query: 191 IFGKIAEEIFALDPQKLMQFSDKALTLTQIQNDK 225
           +FG  AE+   LD +K+   S     L Q++  K
Sbjct: 184 LFGNQAEKF--LDIEKIATLSSSHKLLIQVKASK 215
[Close]
BLAST of Spo21908.1 vs. NCBI nr
Match: gi|1009110792|ref|XP_015897626.1| (PREDICTED: replication protein A 70 kDa DNA-binding subunit B-like [Ziziphus jujuba])

HSP 1 Score: 138.7 bits (348), Expect = 1.700e-29
Identity = 73/212 (34.43%), Postives = 110/212 (51.89%), Query Frame = 1 

		  

Query: 1   MLIIDQELKPIMLTLWDDFASKEGQKIAQMIQTKPIIIGMRLKVVSYHGTSLATRSSSTF 60
           M +++  +  +MLT+W+ F + E  +I  ++  KPIIIG RLKV SY+G SL+T+SSS+F
Sbjct: 120 MTLMNDNMDTLMLTMWNQFVNNECTQILNIMNRKPIIIGCRLKVSSYNGISLSTKSSSSF 179

Query: 61  LINISTPESEALQEWLLQNEDKIQVLINKNSEAPLAPSMKEPTADDIIPVNSLITAAKNT 120
           +I    P++ AL  W   N +K   +I              P+ +DII +  +       
Sbjct: 180 VIESEIPQAAALSIWYNANAEKAMDIICGRIGLETKSLSTLPSVEDIITIKMIDNQLNTG 239

Query: 121 SSFWIKAHATVTDFNQKFWYTGCNTCWREIHADHGQTFNCRNCSGQERIVEARCRFNIEL 180
            SFWI  H  VT+  Q FWY  C  C +    +  Q FNC  C   +     RCR  ++L
Sbjct: 240 RSFWINGHIKVTNLIQPFWYLSCEKCTKATRYEFEQRFNCLYCRHDQVKAMPRCRVIVDL 299

Query: 181 KDPTGTISATIFGKIAEEIFALDPQKLMQFSD 213
            D + +++AT+FG  AE+       +LM  SD
Sbjct: 300 IDESSSLNATLFGNQAEKFLGCTAYELMNKSD 331
[Close]
BLAST of Spo21908.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RWP0_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_025630 PE=4 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 1.800e-158
Identity = 288/296 (97.30%), Postives = 288/296 (97.30%), Query Frame = 1 

		  

Query: 1   MLIIDQELKPIMLTLWDDFASKEGQKIAQMIQTKPIIIGMRLKVVSYHGTSLATRSSSTF 60
           MLIIDQELKPIMLTLWDDFASKEGQKIAQMIQTKPIIIGMRLKVVSYHGTSLATRSSSTF
Sbjct: 138 MLIIDQELKPIMLTLWDDFASKEGQKIAQMIQTKPIIIGMRLKVVSYHGTSLATRSSSTF 197

Query: 61  LINISTPESEALQEWLLQNEDKIQVLINKNSEAPLAPSMKEPTADDIIPVNSLITAAKNT 120
           LINISTPESEALQEWLLQNEDKIQVLINKNSEAPLAPSMKEPTADDIIPVNSLITAAKNT
Sbjct: 198 LINISTPESEALQEWLLQNEDKIQVLINKNSEAPLAPSMKEPTADDIIPVNSLITAAKNT 257

Query: 121 SSFWIKAHATVTDFNQKFWYTGCNTCWREIHADHGQTFNCRNCSGQERIVEARCRFNIEL 180
           SSFWIKAHATVTDFNQKFWYTGCNTCWREIHADHGQTFNCRNCSGQERIVEARCRFNIEL
Sbjct: 258 SSFWIKAHATVTDFNQKFWYTGCNTCWREIHADHGQTFNCRNCSGQERIVEARCRFNIEL 317

Query: 181 KDPTGTISATIFGKIAEEIFALDPQKLMQFSDKALTLTQIQNDKIDTAALGICSSTKKYY 240
           KDPTGTISATIFGKIAEEIFALDPQKLMQFSDK        NDKIDTAALGICSSTKKYY
Sbjct: 318 KDPTGTISATIFGKIAEEIFALDPQKLMQFSDK--------NDKIDTAALGICSSTKKYY 377

Query: 241 FQLKPNRNNENQYIITKALDKLQQDHAEDAQKKTPLKQEANENSVHQQQIKDNTST 297
           FQLKPNRNNENQYIITKALDKLQQDHAEDAQKKTPLKQEANENSVHQQQIKDNTST
Sbjct: 378 FQLKPNRNNENQYIITKALDKLQQDHAEDAQKKTPLKQEANENSVHQQQIKDNTST 425
[Close]
BLAST of Spo21908.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RV40_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_025630 PE=4 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 1.800e-158
Identity = 288/296 (97.30%), Postives = 288/296 (97.30%), Query Frame = 1 

		  

Query: 1   MLIIDQELKPIMLTLWDDFASKEGQKIAQMIQTKPIIIGMRLKVVSYHGTSLATRSSSTF 60
           MLIIDQELKPIMLTLWDDFASKEGQKIAQMIQTKPIIIGMRLKVVSYHGTSLATRSSSTF
Sbjct: 1   MLIIDQELKPIMLTLWDDFASKEGQKIAQMIQTKPIIIGMRLKVVSYHGTSLATRSSSTF 60

Query: 61  LINISTPESEALQEWLLQNEDKIQVLINKNSEAPLAPSMKEPTADDIIPVNSLITAAKNT 120
           LINISTPESEALQEWLLQNEDKIQVLINKNSEAPLAPSMKEPTADDIIPVNSLITAAKNT
Sbjct: 61  LINISTPESEALQEWLLQNEDKIQVLINKNSEAPLAPSMKEPTADDIIPVNSLITAAKNT 120

Query: 121 SSFWIKAHATVTDFNQKFWYTGCNTCWREIHADHGQTFNCRNCSGQERIVEARCRFNIEL 180
           SSFWIKAHATVTDFNQKFWYTGCNTCWREIHADHGQTFNCRNCSGQERIVEARCRFNIEL
Sbjct: 121 SSFWIKAHATVTDFNQKFWYTGCNTCWREIHADHGQTFNCRNCSGQERIVEARCRFNIEL 180

Query: 181 KDPTGTISATIFGKIAEEIFALDPQKLMQFSDKALTLTQIQNDKIDTAALGICSSTKKYY 240
           KDPTGTISATIFGKIAEEIFALDPQKLMQFSDK        NDKIDTAALGICSSTKKYY
Sbjct: 181 KDPTGTISATIFGKIAEEIFALDPQKLMQFSDK--------NDKIDTAALGICSSTKKYY 240

Query: 241 FQLKPNRNNENQYIITKALDKLQQDHAEDAQKKTPLKQEANENSVHQQQIKDNTST 297
           FQLKPNRNNENQYIITKALDKLQQDHAEDAQKKTPLKQEANENSVHQQQIKDNTST
Sbjct: 241 FQLKPNRNNENQYIITKALDKLQQDHAEDAQKKTPLKQEANENSVHQQQIKDNTST 288
[Close]
BLAST of Spo21908.1 vs. UniProtKB/TrEMBL
Match: A0A068U942_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00018731001 PE=4 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 4.800e-31
Identity = 85/272 (31.25%), Postives = 146/272 (53.68%), Query Frame = 1 

		  

Query: 2   LIIDQELKPIMLTLWDDFASKEGQKIAQMIQTKPIIIGMRLKVVSYHGTSLATRSSSTFL 61
           +++++E KP++LTLW++F   +G ++A  I    +IIGM+LK+ +++  SL T+  S  L
Sbjct: 215 IVVNEEKKPMLLTLWNEFEQNQGTQLANSIGNANVIIGMKLKITTFNYLSLTTKPGSGLL 274

Query: 62  INISTPESEALQEWLLQNEDKIQVLINKNSEAPLAPSMKEPTADDIIPVNSLITAAKNTS 121
           IN ST E+ AL+EW   N+++I  LI + +    +  +  P A+DII V + +   K+  
Sbjct: 275 INPSTSEANALKEWYNANKEEIAELIQQIAYKDSSKLLPPPIANDIISVANALNTLKDVK 334

Query: 122 SFWIKAHATVTDFNQKFWYTGCNTCWREIHADHGQTFNCRNCSGQERIVEARCRFNIELK 181
           + WI    +++   QKFW+  C  C + I+ D G    C +C    R+V AR R  I + 
Sbjct: 335 TAWITGKISLSPGQQKFWFEACENCQKTINVDVGWVMRCPSCKEDSRVV-ARTRVGIAVD 394

Query: 182 DPTGTISATIFGKIAEEIFALDPQKLMQFSDKALTLTQIQNDKIDTAALGICSSTKKY-- 241
           D TG+I+  IFG        LD +KL+ F+  AL   +   ++++ +A  + S+ +K+  
Sbjct: 395 DGTGSINTVIFG--------LDAEKLIPFT--ALQFWEAHTEELNFSA-ELASALRKHAI 454

Query: 242 -----YFQLKPNRNNENQYIITKALDKLQQDH 267
                Y++       E +Y I KA    +  H
Sbjct: 455 VCFIRYYESDYQGRKEAKYNIVKAYTTEESAH 474
[Close]
BLAST of Spo21908.1 vs. UniProtKB/TrEMBL
Match: A0A068UXN1_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00035908001 PE=4 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 7.900e-26
Identity = 77/255 (30.20%), Postives = 133/255 (52.16%), Query Frame = 1 

		  

Query: 11  IMLTLWDDFASKEGQKIAQMIQTKPIIIGMRLKVVSYHGTSLATRSSSTFLINISTPESE 70
           ++LTLW++F   +G  I   I    +IIG++LK+ +++  SL T+  S+ LIN  T E+ 
Sbjct: 1   MLLTLWNEFEQNQGTHITNSIGNANVIIGIKLKITTFNYLSLTTKPGSSHLINPPTSEAN 60

Query: 71  ALQEWLLQNEDKIQVLINKNSEAPLAPSMKEPTADDIIPVNSLITAAKNTSSFWIKAHAT 130
            L+EW   N+++I  LI + +    +  +  P+A+DII V + +   K+  + WI    +
Sbjct: 61  VLKEWYNANKEEITELIQQMAYKDSSKLLPSPSANDIISVANALNTLKDVKTAWIIGKIS 120

Query: 131 VTDFNQKFWYTGCNTCWREIHADHGQTFNCRNCSGQERIVEARCRFNIELKDPTGTISAT 190
           +T   QKFW+  C  C + I+ D G    C  C  + R+V AR +  I + D TG+I+ +
Sbjct: 121 LTPGQQKFWFETCENCQKAINVDVGCVMRCPFCKEESRVV-ARTQIGIAVDDGTGSINTS 180

Query: 191 IFGKIAEEIFALDPQKLMQFSDKALTLTQIQNDKIDTAALGICSSTKKY-------YFQL 250
           IFG        LD +KL  F+  AL   +   ++++  A  + S+ +++       Y++ 
Sbjct: 181 IFG--------LDAEKLTPFT--ALQFWEAHTEELNFFA-ELASAIRRHLIVCFIRYYES 240

Query: 251 KPNRNNENQYIITKA 259
                 E +Y I KA
Sbjct: 241 NYQGQKEGKYNIVKA 243
[Close]
BLAST of Spo21908.1 vs. UniProtKB/TrEMBL
Match: A0A068USR3_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00031434001 PE=4 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 1.500e-24
Identity = 70/207 (33.82%), Postives = 112/207 (54.11%), Query Frame = 1 

		  

Query: 11  IMLTLWDDFASKEGQKIAQMIQTKPIIIGMRLKVVSYHGTSLATRSSSTFLINISTPESE 70
           ++L+LWDDF + EGQ +   +Q+ P+IIG RLKV +Y+G SL+T   S  L++    E+ 
Sbjct: 60  VLLSLWDDFLNNEGQILLNNMQSYPVIIGRRLKVNNYNGVSLSTWFDSALLVDPPIQEAR 119

Query: 71  ALQEWLLQNEDKIQVLINKNSEAPLAPSMKEPTADDIIPVNSLITAAKNTSSFWIKAHAT 130
            L+ W ++N   I  +IN  S     P++     D    +   +T+++ T+  W+KA  +
Sbjct: 120 QLKNWAMRNAKSIAEIINAKSYIKYNPAL-SLKRDQKTTLICNVTSSQKTA--WVKAKLS 179

Query: 131 VTDFNQKFWYTGCNTCWREIHADHGQTFNCRNCSGQERIVEARCRFNIELKDPTGTISAT 190
                QK+ Y  C  C+R   AD+G  F C +C  ++     RCRF+++L D +G I A+
Sbjct: 180 FEHIFQKYCYMSCVKCYRATAADYGIEFTCNSCK-EKGPAMPRCRFDVDLTDDSGVIPAS 239

Query: 191 IFGKIAEEIFALDPQKLMQFSDKALTL 218
           IFG  AE I      + M   ++ L L
Sbjct: 240 IFGDPAETILTFTGLEAMDQFNQNLEL 262
[Close]
BLAST of Spo21908.1 vs. ExPASy Swiss-Prot
Match: RFA1B_ORYSJ (Replication protein A 70 kDa DNA-binding subunit B OS=Oryza sativa subsp. japonica GN=RPA1B PE=1 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 6.300e-16
Identity = 56/213 (26.29%), Postives = 96/213 (45.07%), Query Frame = 1 

		  

Query: 1   MLIIDQELKPIMLTLWDDFASKEGQKIAQMIQTKPIIIGMRLKVVSYHGTSLATRSSSTF 60
           +++ D   K + ++LW+D A+  GQ++  M+ + PII    LKV  + G SL+T   ST 
Sbjct: 350 IVVADDSSKTVTISLWNDLATTTGQELLDMVDSAPIIAIKSLKVSDFQGLSLSTVGRSTI 409

Query: 61  LINISTPESEALQEWL-LQNEDKIQVLINKNSEAPLAPSMKEPTADDIIPVNSLITAAKN 120
           ++N   PE+E L+ W   + +      I  +  A      +   +D +    S IT+  N
Sbjct: 410 VVNPDLPEAEQLRAWYDSEGKGTSMASIGSDMGASRVGGARSMYSDRVF--LSHITSDPN 469

Query: 121 TSS-----FWIKAHATVTDFNQKFWYTGCNTCWREIHADHGQTFNCRNCSGQERIVEARC 180
                   F + A+ ++   +Q  WY  C TC +++    G  + C  C   +     R 
Sbjct: 470 LGQDKPVFFSLNAYISLIKPDQTMWYRACKTCNKKVTEAIGSGYWCEGCQKNDAECSLRY 529

Query: 181 RFNIELKDPTGTISATIFGKIAEEIFALDPQKL 208
              I++ DPTG    ++F   AE I      +L
Sbjct: 530 IMVIKVSDPTGEAWLSLFNDQAERIVGCSADEL 560
[Close]
BLAST of Spo21908.1 vs. ExPASy Swiss-Prot
Match: RFA1B_ARATH (Replication protein A 70 kDa DNA-binding subunit B OS=Arabidopsis thaliana GN=RPA1B PE=3 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 3.200e-12
Identity = 51/224 (22.77%), Postives = 98/224 (43.75%), Query Frame = 1 

		  

Query: 3   IIDQELKPIMLTLWDDFASKEGQKIAQMIQTKPIIIGMRLKVVSYHGTSLATRSSSTFLI 62
           + D+  K ++++LW+D A+  GQ++  M    P+I    LKV ++ G SL+T S S  +I
Sbjct: 324 LADETKKTVVVSLWNDLATGIGQELLDMADNHPVIAIKSLKVGAFQGVSLSTISRSNVVI 383

Query: 63  NISTPESEALQEWL-LQNEDKIQVLINKNSEAPLAPSMKEPTADDI----IPVNSLITAA 122
           N ++PE+  L+ W   + ++     I     +      +   +D +    I  N  +   
Sbjct: 384 NPNSPEATKLKSWYDAEGKETSMSAIGSGMSSSANNGSRSMYSDRVFLSHITSNPSLGEE 443

Query: 123 KNTSSFWIKAHATVTDFNQKFWYTGCNTCWREIHADHGQTFNCRNCSGQERIVEARCRFN 182
           K    F  +A+ +    +Q  WY  C TC +++       + C +C  +++    R    
Sbjct: 444 KPV-FFSTRAYISFIKPDQTMWYRACKTCNKKVTEAMDSGYWCESCQKKDQECSLRYIMA 503

Query: 183 IELKDPTGTISATIFGKIAEEIFALDPQKLMQFSDKALTLTQIQ 222
           +++ D TG    + F   AE+I       L     +   + + Q
Sbjct: 504 VKVSDSTGETWLSAFNDEAEKIIGCTADDLNDLKSEEGEVNEFQ 546
[Close]
BLAST of Spo21908.1 vs. ExPASy Swiss-Prot
Match: RFA1A_ORYSJ (Replication protein A 70 kDa DNA-binding subunit A OS=Oryza sativa subsp. japonica GN=RPA1A PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 4.800e-8
Identity = 49/234 (20.94%), Postives = 94/234 (40.17%), Query Frame = 1 

		  

Query: 1   MLIIDQELKPIMLTLWDDFASKEGQKIAQMIQTK--PIIIGMRLKVVSYHGTSLATRSSS 60
           M + D   + + +T+W DF ++EG ++  M++    P++     KV  + G S+ T SS+
Sbjct: 374 MNLKDMSGRSVEVTMWGDFCNREGSQLQGMVERGIFPVLAVKAGKVSDFSGKSVGTISST 433

Query: 61  TFLINISTPESEALQEWLLQNEDKIQVLINKNSEAPLAPSMKEPTADDIIPVNSLITAAK 120
              IN  + E+ +L++W                ++    +  +  + DI P  S     K
Sbjct: 434 QLFINPDSAEAHSLRQWF---------------DSGGRDASTQSISRDITPGASRNEIRK 493

Query: 121 NTSSF------------WIKAHATVTDF-NQKFWYTGC------NTCWREIHADHGQTFN 180
             +              WI   ATV  F N+ F+YT C        C +++       + 
Sbjct: 494 TVAQIKDEGLGMGDKPDWITVKATVIFFKNESFFYTACPNMIGDRQCNKKVTKSTNGNWT 553

Query: 181 CRNCSGQERIVEARCRFNIELKDPTGTISATIFGKIAEEIFALDPQKLMQFSDK 214
           C  C  +    + R     +++D +GT   T F +  +E+      +L    ++
Sbjct: 554 CDKCDREFEECDYRYLLQFQIQDHSGTAWVTAFQEAGQELLGCSATELNALKER 592
[Close]
BLAST of Spo21908.1 vs. ExPASy Swiss-Prot
Match: RFA1C_ORYSJ (Replication protein A 70 kDa DNA-binding subunit C OS=Oryza sativa subsp. japonica GN=RPA1C PE=1 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 1.200e-6
Identity = 49/202 (24.26%), Postives = 85/202 (42.08%), Query Frame = 1 

		  

Query: 9   KPIMLTLWDDFASKEGQKIAQMIQT--KPIIIGMRLKVVSYHGTSLATRSSSTFLINIST 68
           + I +TLW +F   EGQ++     +   PII     +V  ++G S++T  S+  +IN   
Sbjct: 479 RSIEVTLWGNFCDAEGQQLQLQCDSGSNPIIAFKGARVGDFNGKSVSTIGSTQLIINPDF 538

Query: 69  PESEALQEWLLQNEDKIQVLINKNSEAPLAPSMKEPTADDIIPVNSLITAAKNTSSFWIK 128
           PE E L++W +  E K    I+ + E     +M    A   I         +     WI 
Sbjct: 539 PEVERLRQWYM-TEGKTAPCISLSRE---MLNMGRTDARKTIAQIKDENLGRLEKPDWIT 598

Query: 129 AHATVTDF-NQKFWYTGC-------NTCWREIHADHGQTFNCRNCSGQERIVEARCRFNI 188
             A ++    + F Y  C         C ++   +    ++C  C    +  E R     
Sbjct: 599 VKAAISHVTTESFCYPACPKLLPVGRQCNKKAINNGDGMWHCDRCDESFQNPEYRYMLRF 658

Query: 189 ELKDPTGTISATIFGKIAEEIF 201
           +++D TG+  A+ F +  E+IF
Sbjct: 659 QIQDHTGSTYASAFDEAGEQIF 676
[Close]
BLAST of Spo21908.1 vs. TAIR (Arabidopsis)
Match: AT5G08020.1 (RPA70-kDa subunit B)

HSP 1 Score: 73.9 bits (180), Expect = 1.800e-13
Identity = 51/224 (22.77%), Postives = 98/224 (43.75%), Query Frame = 1 

		  

Query: 3   IIDQELKPIMLTLWDDFASKEGQKIAQMIQTKPIIIGMRLKVVSYHGTSLATRSSSTFLI 62
           + D+  K ++++LW+D A+  GQ++  M    P+I    LKV ++ G SL+T S S  +I
Sbjct: 324 LADETKKTVVVSLWNDLATGIGQELLDMADNHPVIAIKSLKVGAFQGVSLSTISRSNVVI 383

Query: 63  NISTPESEALQEWL-LQNEDKIQVLINKNSEAPLAPSMKEPTADDI----IPVNSLITAA 122
           N ++PE+  L+ W   + ++     I     +      +   +D +    I  N  +   
Sbjct: 384 NPNSPEATKLKSWYDAEGKETSMSAIGSGMSSSANNGSRSMYSDRVFLSHITSNPSLGEE 443

Query: 123 KNTSSFWIKAHATVTDFNQKFWYTGCNTCWREIHADHGQTFNCRNCSGQERIVEARCRFN 182
           K    F  +A+ +    +Q  WY  C TC +++       + C +C  +++    R    
Sbjct: 444 KPV-FFSTRAYISFIKPDQTMWYRACKTCNKKVTEAMDSGYWCESCQKKDQECSLRYIMA 503

Query: 183 IELKDPTGTISATIFGKIAEEIFALDPQKLMQFSDKALTLTQIQ 222
           +++ D TG    + F   AE+I       L     +   + + Q
Sbjct: 504 VKVSDSTGETWLSAFNDEAEKIIGCTADDLNDLKSEEGEVNEFQ 546
The following BLAST results are available for this feature:
BLAST of Spo21908.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902234132|gb|KNA23339.1|2.5e-15897.3hypothetical protein SOVF_0256... [more]
gi|902234131|gb|KNA23338.1|2.5e-15897.3hypothetical protein SOVF_0256... [more]
gi|661891754|emb|CDP04679.1|6.9e-3131.2unnamed protein product [Coffe... [more]
gi|1009110771|ref|XP_015897487.1|1.3e-2935.5PREDICTED: replication protein... [more]
gi|1009110792|ref|XP_015897626.1|1.7e-2934.4PREDICTED: replication protein... [more]
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BLAST of Spo21908.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9RWP0_SPIOL1.8e-15897.3Uncharacterized protein OS=Spi... [more]
A0A0K9RV40_SPIOL1.8e-15897.3Uncharacterized protein OS=Spi... [more]
A0A068U942_COFCA4.8e-3131.2Uncharacterized protein OS=Cof... [more]
A0A068UXN1_COFCA7.9e-2630.2Uncharacterized protein OS=Cof... [more]
A0A068USR3_COFCA1.5e-2433.8Uncharacterized protein OS=Cof... [more]
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BLAST of Spo21908.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 4
Match NameE-valueIdentityDescription
RFA1B_ORYSJ6.3e-1626.2Replication protein A 70 kDa D... [more]
RFA1B_ARATH3.2e-1222.7Replication protein A 70 kDa D... [more]
RFA1A_ORYSJ4.8e-820.9Replication protein A 70 kDa D... [more]
RFA1C_ORYSJ1.2e-624.2Replication protein A 70 kDa D... [more]
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BLAST of Spo21908.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1
Match NameE-valueIdentityDescription
AT5G08020.11.8e-1322.7RPA70-kDa subunit B[more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012340Nucleic acid-binding, OB-foldGENE3D2.40.50.140coord: 3..81
score: 3.4E-10coord: 136..270
score: 2.7
IPR012340Nucleic acid-binding, OB-foldunknownSSF50249Nucleic acid-binding proteinscoord: 2..79
score: 1.11E-5coord: 117..253
score: 4.71
IPR013955Replication factor A, C-terminalPFAMPF08646Rep_fac-A_Ccoord: 124..244
score: 7.5
NoneNo IPR availablePANTHERPTHR23273REPLICATION FACTOR A 1, RFA1coord: 5..211
score: 4.5
NoneNo IPR availablePANTHERPTHR23273:SF15REPLICATION PROTEIN A 70 KDA DNA-BINDING SUBUNIT Acoord: 5..211
score: 4.5

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0036092 phosphatidylinositol-3-phosphate biosynthetic process
biological_process GO:0006355 regulation of transcription, DNA-templated
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0009755 hormone-mediated signaling pathway
biological_process GO:1901701 cellular response to oxygen-containing compound
biological_process GO:0071229 cellular response to acid chemical
biological_process GO:0044237 cellular metabolic process
biological_process GO:0009069 serine family amino acid metabolic process
biological_process GO:0009607 response to biotic stimulus
biological_process GO:0006952 defense response
biological_process GO:0007033 vacuole organization
biological_process GO:0007018 microtubule-based movement
biological_process GO:0006468 protein phosphorylation
biological_process GO:0010218 response to far red light
biological_process GO:0009585 red, far-red light phototransduction
biological_process GO:0008284 positive regulation of cell proliferation
biological_process GO:0008152 metabolic process
biological_process GO:0009630 gravitropism
biological_process GO:0006487 protein N-linked glycosylation
biological_process GO:0000956 nuclear-transcribed mRNA catabolic process
biological_process GO:0042753 positive regulation of circadian rhythm
biological_process GO:0045893 positive regulation of transcription, DNA-templated
biological_process GO:0009628 response to abiotic stimulus
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005667 transcription factor complex
cellular_component GO:0005871 kinesin complex
cellular_component GO:0005874 microtubule
cellular_component GO:0045298 tubulin complex
cellular_component GO:0005634 nucleus
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0008017 microtubule binding
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0005515 protein binding
molecular_function GO:0005516 calmodulin binding
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0005488 binding
molecular_function GO:0008568 microtubule-severing ATPase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0042578 phosphoric ester hydrolase activity
molecular_function GO:0003777 microtubule motor activity
RNA-Seq Expression