Spo22466 (gene)

Overview
NameSpo22466
Typegene
OrganismSpinacia oleracea (Spinach)
DescriptionrRNA intron-encoded homing endonuclease
LocationSpoScf_26019 : 123 .. 1352 (+)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAGGGCTCATGCGTGCCGGTGTCGATCGAGGAGTGCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACTAATTCAGACTGTGAAACTGCGAATGGCTCATTAAATCAGTTATAGTTTGTTTGATGGTACCTGCTACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAACAAACCCCGACTTCTGGAAGGGACGCATTTATTAGATAAAAGGTCAACGCGGGCTTTTAGCCCGTTGCTCTGATGATTCATGATAACTCGACGGATCGCACGGCCTTTGCGCCGGCGACGCATCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCCTACCATGGTGGTGACGGGTGACGGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACGGGGAGGTAGTGACAATAAATAACAATACCGGGCTCATTGAGTCTGGTAATTGGAATGAGTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCTAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGACCTTGGGGTGGTACGACCGGTCCGCCTTTTGGTGTGCACCGGCCGTCTCGCCTCTTTCGCCGGCGATGCGCTCCTGGCCTTAATTGGCCGGGTCGTGCCTCCGGCACTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGTATACATTAGCATGGGATAACATTATAGGATTCCGGTCCTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGAATAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTTAGGACGCCGCCGGCACCTTATGAGATATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAA

mRNA sequence

ATGGGAGGGCTCATGCGTGCCGGTGTCGATCGAGGAGTGCTACCTGTTAAAAAGCTCGTAGTTGGACCTTGGGGTGGTACGACCGGTCCGCCTTTTGGTGTGCACCGGCCGTCTCGCCTCTTTCGCCGGCGATGCGCTCCTGGCCTTAATTGGCCGGGTCGTGCCTCCGGCACTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGTATACATTAGCATGGGATAACATTATAGGATTCCGACGAATAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTTAGGACGCCGCCGGCACCTTATGAGATATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAA

Coding sequence (CDS)

ATGGGAGGGCTCATGCGTGCCGGTGTCGATCGAGGAGTGCTACCTGTTAAAAAGCTCGTAGTTGGACCTTGGGGTGGTACGACCGGTCCGCCTTTTGGTGTGCACCGGCCGTCTCGCCTCTTTCGCCGGCGATGCGCTCCTGGCCTTAATTGGCCGGGTCGTGCCTCCGGCACTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGTATACATTAGCATGGGATAACATTATAGGATTCCGACGAATAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTTAGGACGCCGCCGGCACCTTATGAGATATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAA

Protein sequence

MGGLMRAGVDRGVLPVKKLVVGPWGGTTGPPFGVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWDNIIGFRRITAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEISKFLGSGGSMVARLKLKGIDGRAPPGVEPAA
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo22466.1Spo22466.1mRNA


Homology
[Close]
BLAST of Spo22466.1 vs. NCBI nr
Match: gi|703137890|ref|XP_010106597.1| (hypothetical protein L484_002552 [Morus notabilis])

HSP 1 Score: 260.0 bits (663), Expect = 2.900e-66
Identity = 129/138 (93.48%), Postives = 130/138 (94.20%), Query Frame = 1 

		  

Query: 33  GVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWDNIIGFRRITAKAF 92
           GVHR +R F RRCAPGLNWPGRASG VTLKKLECSKQAYALYTLAWDNIIGFRR TAKAF
Sbjct: 55  GVHRSARPFCRRCAPGLNWPGRASGAVTLKKLECSKQAYALYTLAWDNIIGFRRTTAKAF 114

Query: 93  AKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEISKFLGSGGSMVAR 152
           AKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTP APYE SKFLGSGGSMVAR
Sbjct: 115 AKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKFLGSGGSMVAR 174

Query: 153 LKLKGIDGRAPPGVEPAA 171
           LKLKGIDGRAPPGVEPAA
Sbjct: 175 LKLKGIDGRAPPGVEPAA 192
[Close]
BLAST of Spo22466.1 vs. NCBI nr
Match: gi|823127874|ref|XP_012440607.1| (PREDICTED: uncharacterized protein LOC105765883 [Gossypium raimondii])

HSP 1 Score: 223.0 bits (567), Expect = 3.900e-55
Identity = 116/152 (76.32%), Postives = 121/152 (79.61%), Query Frame = 1 

		  

Query: 16  VKKLVVGPWGGTTGPPFGVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYT 75
           VKKLVVG  GG+ GPP G HR +R + RRCAPGLNWPGR+S  VTLKKLECSKQAYA   
Sbjct: 40  VKKLVVGLRGGSAGPPHGEHRSARPYCRRCAPGLNWPGRSSDAVTLKKLECSKQAYA--- 99

Query: 76  LAWDNIIGFRRITAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPA 135
                          +FAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTPPA
Sbjct: 100 ---------------SFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPPA 159

Query: 136 PYEISKFLGSGGSMVARLKLKGIDGRAPPGVE 168
           PYE SK LGSGGSMVARLKLKGIDGRAPPGVE
Sbjct: 160 PYEKSKSLGSGGSMVARLKLKGIDGRAPPGVE 173
[Close]
BLAST of Spo22466.1 vs. NCBI nr
Match: gi|694380941|ref|XP_009366572.1| (PREDICTED: uncharacterized protein LOC103956333 [Pyrus x bretschneideri])

HSP 1 Score: 219.9 bits (559), Expect = 3.300e-54
Identity = 116/142 (81.69%), Postives = 119/142 (83.80%), Query Frame = 1 

		  

Query: 33  GVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWD-----NIIGFRRI 92
           GVHR +R F RR APGLNWPGRASG VTLKKLECSKQ +    LA+      N     R 
Sbjct: 228 GVHRSARPFCRRYAPGLNWPGRASGAVTLKKLECSKQDFGPILLAFGIGVMINRDSRGRT 287

Query: 93  TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEISKFLGSGG 152
           TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYE SKFLGSGG
Sbjct: 288 TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLGSGG 347

Query: 153 SMVARLKLKGIDGRAPPGVEPA 170
           SMVARLKLKGIDGRAPPGVEPA
Sbjct: 348 SMVARLKLKGIDGRAPPGVEPA 369
[Close]
BLAST of Spo22466.1 vs. NCBI nr
Match: gi|922327155|ref|XP_013442970.1| (hypothetical protein MTR_0055s0130 [Medicago truncatula])

HSP 1 Score: 213.8 bits (543), Expect = 2.400e-52
Identity = 114/143 (79.72%), Postives = 117/143 (81.82%), Query Frame = 1 

		  

Query: 32  FGVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWD-----NIIGFRR 91
           +GVHR +R F RRCAPGLNWPGRASG VTLKKLECSKQ      LA+      N     R
Sbjct: 485 YGVHRLARPFCRRCAPGLNWPGRASGAVTLKKLECSKQDSDPIVLAFGIGVMINRDSRGR 544

Query: 92  ITAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEISKFLGSG 151
            TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTP APYE SK LGSG
Sbjct: 545 TTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKSLGSG 604

Query: 152 GSMVARLKLKGIDGRAPPGVEPA 170
           GSMVARLKLKGIDGRAPPGVEPA
Sbjct: 605 GSMVARLKLKGIDGRAPPGVEPA 627
[Close]
BLAST of Spo22466.1 vs. NCBI nr
Match: gi|947068904|gb|KRH17795.1| (hypothetical protein GLYMA_13G016700 [Glycine max])

HSP 1 Score: 211.1 bits (536), Expect = 1.500e-51
Identity = 114/143 (79.72%), Postives = 116/143 (81.12%), Query Frame = 1 

		  

Query: 33  GVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWD-----NIIGFRRI 92
           GVHR +R F RRCAP LNWPGRASG VTLKKLECSKQ      LA+      N     R 
Sbjct: 128 GVHRSARPFCRRCAPVLNWPGRASGAVTLKKLECSKQDSDPIVLAFGIGVMINRDSRGRT 187

Query: 93  TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEISKFLGSGG 152
           TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTP APYE SK LGSGG
Sbjct: 188 TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKSLGSGG 247

Query: 153 SMVARLKLKGIDGRAPPGVEPAA 171
           SMVARLKLKGIDGRAPPGVEPAA
Sbjct: 248 SMVARLKLKGIDGRAPPGVEPAA 270
[Close]
BLAST of Spo22466.1 vs. UniProtKB/TrEMBL
Match: M1DUP9_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400044276 PE=4 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 2.400e-67
Identity = 131/141 (92.91%), Postives = 131/141 (92.91%), Query Frame = 1 

		  

Query: 30  PPFGVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWDNIIGFRRITA 89
           P   VHR SR F RRCAPGLNWPGRASG VTLKKLECSKQAYALYTLAWDNIIGFRR TA
Sbjct: 45  PCICVHRSSRPFCRRCAPGLNWPGRASGAVTLKKLECSKQAYALYTLAWDNIIGFRRTTA 104

Query: 90  KAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEISKFLGSGGSM 149
           KAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTPPAPYE SKFLGSGGSM
Sbjct: 105 KAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPPAPYEKSKFLGSGGSM 164

Query: 150 VARLKLKGIDGRAPPGVEPAA 171
           VARLKLKGIDGRAPPGVEPAA
Sbjct: 165 VARLKLKGIDGRAPPGVEPAA 185
[Close]
BLAST of Spo22466.1 vs. UniProtKB/TrEMBL
Match: W9SJ11_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_002552 PE=4 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 2.000e-66
Identity = 129/138 (93.48%), Postives = 130/138 (94.20%), Query Frame = 1 

		  

Query: 33  GVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWDNIIGFRRITAKAF 92
           GVHR +R F RRCAPGLNWPGRASG VTLKKLECSKQAYALYTLAWDNIIGFRR TAKAF
Sbjct: 55  GVHRSARPFCRRCAPGLNWPGRASGAVTLKKLECSKQAYALYTLAWDNIIGFRRTTAKAF 114

Query: 93  AKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEISKFLGSGGSMVAR 152
           AKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTP APYE SKFLGSGGSMVAR
Sbjct: 115 AKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKFLGSGGSMVAR 174

Query: 153 LKLKGIDGRAPPGVEPAA 171
           LKLKGIDGRAPPGVEPAA
Sbjct: 175 LKLKGIDGRAPPGVEPAA 192
[Close]
BLAST of Spo22466.1 vs. UniProtKB/TrEMBL
Match: M1DB49_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400035737 PE=4 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 1.400e-62
Identity = 122/130 (93.85%), Postives = 124/130 (95.38%), Query Frame = 1 

		  

Query: 41  FRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWDNIIGFRRITAKAFAKDVFINQ 100
           F RRCAPGLNWPGRASG VTLKKLECSKQAYALYTLAWDNIIGFRR TAKAFAKDVFINQ
Sbjct: 5   FCRRCAPGLNWPGRASGAVTLKKLECSKQAYALYTLAWDNIIGFRRTTAKAFAKDVFINQ 64

Query: 101 ERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEISKFLGSGGSMVARLKLKGIDG 160
           ERKLGARRRSDTVLVSTINDADQGSADV FRTPPAPYE SKFLGSGGS+VARLKLKGIDG
Sbjct: 65  ERKLGARRRSDTVLVSTINDADQGSADVAFRTPPAPYEKSKFLGSGGSIVARLKLKGIDG 124

Query: 161 RAPPGVEPAA 171
           +APPGVE AA
Sbjct: 125 KAPPGVELAA 134
[Close]
BLAST of Spo22466.1 vs. UniProtKB/TrEMBL
Match: A0A0D2ZQ67_BRAOL (Uncharacterized protein OS=Brassica oleracea var. oleracea PE=4 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 3.500e-58
Identity = 119/142 (83.80%), Postives = 123/142 (86.62%), Query Frame = 1 

		  

Query: 33  GVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWDNIIGFRRITA--- 92
           G+HR +  F R+ APGLNWPGRASG VTLKKLECSKQAY LYTLAWDNIIGF    A   
Sbjct: 189 GMHRSACPFCRQYAPGLNWPGRASGAVTLKKLECSKQAYTLYTLAWDNIIGFLSYCAGLQ 248

Query: 93  -KAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEISKFLGSGGS 152
            + FAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTP APYE SKFLGSGGS
Sbjct: 249 DRTFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKFLGSGGS 308

Query: 153 MVARLKLKGIDGRAPPGVEPAA 171
           MVARLKLKGIDGRAPPGVEPAA
Sbjct: 309 MVARLKLKGIDGRAPPGVEPAA 330
[Close]
BLAST of Spo22466.1 vs. UniProtKB/TrEMBL
Match: A0A072TTW7_MEDTR (Uncharacterized protein OS=Medicago truncatula GN=MTR_0055s0130 PE=4 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 1.700e-52
Identity = 114/143 (79.72%), Postives = 117/143 (81.82%), Query Frame = 1 

		  

Query: 32  FGVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWD-----NIIGFRR 91
           +GVHR +R F RRCAPGLNWPGRASG VTLKKLECSKQ      LA+      N     R
Sbjct: 485 YGVHRLARPFCRRCAPGLNWPGRASGAVTLKKLECSKQDSDPIVLAFGIGVMINRDSRGR 544

Query: 92  ITAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEISKFLGSG 151
            TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTP APYE SK LGSG
Sbjct: 545 TTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKSLGSG 604

Query: 152 GSMVARLKLKGIDGRAPPGVEPA 170
           GSMVARLKLKGIDGRAPPGVEPA
Sbjct: 605 GSMVARLKLKGIDGRAPPGVEPA 627
The following BLAST results are available for this feature:
BLAST of Spo22466.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|703137890|ref|XP_010106597.1|2.9e-6693.4hypothetical protein L484_0025... [more]
gi|823127874|ref|XP_012440607.1|3.9e-5576.3PREDICTED: uncharacterized pro... [more]
gi|694380941|ref|XP_009366572.1|3.3e-5481.6PREDICTED: uncharacterized pro... [more]
gi|922327155|ref|XP_013442970.1|2.4e-5279.7hypothetical protein MTR_0055s... [more]
gi|947068904|gb|KRH17795.1|1.5e-5179.7hypothetical protein GLYMA_13G... [more]
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BLAST of Spo22466.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
M1DUP9_SOLTU2.4e-6792.9Uncharacterized protein OS=Sol... [more]
W9SJ11_9ROSA2.0e-6693.4Uncharacterized protein OS=Mor... [more]
M1DB49_SOLTU1.4e-6293.8Uncharacterized protein OS=Sol... [more]
A0A0D2ZQ67_BRAOL3.5e-5883.8Uncharacterized protein OS=Bra... [more]
A0A072TTW7_MEDTR1.7e-5279.7Uncharacterized protein OS=Med... [more]
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BLAST of Spo22466.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Spo22466.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 0
Match NameE-valueIdentityDescription
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR34410FAMILY NOT NAMEDcoord: 75..170
score: 1.8
NoneNo IPR availablePANTHERPTHR34410:SF2SUBFAMILY NOT NAMEDcoord: 75..170
score: 1.8

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006506 GPI anchor biosynthetic process
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0009772 photosynthetic electron transport in photosystem II
biological_process GO:0009635 response to herbicide
cellular_component GO:0009535 chloroplast thylakoid membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009523 photosystem II
cellular_component GO:0005739 mitochondrion
molecular_function GO:0004519 endonuclease activity
molecular_function GO:0016758 transferase activity, transferring hexosyl groups
molecular_function GO:0016168 chlorophyll binding
molecular_function GO:0045156 electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity
molecular_function GO:0005506 iron ion binding
molecular_function GO:0010242 oxygen evolving activity
RNA-Seq Expression
   



Co-expression
Gener valueExpression
Spo161960.71Barchart | Table
Spo111220.69Barchart | Table