Spo22897 (gene) | SpinachBase

Overview


Name	Spo22897
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Chaperone protein ClpB1 (ATP-dependent Clp protease ATP-binding subunit ClpB homolog 1) (Casein lytic proteinase B1) (Heat shock protein 101)
Location	SpoScf_01568 : 106443 .. 109707 (+)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACATCTAATCTTGAGGTGGAGCATTTCCTCTCTGGATTCATGGGCAAGAGCACCATGGAGGCAGCTCAAGAGATCGAAAGTTATGGCACAGGTATTGATCTTTGAAACCAAAATATTTCTTAGTGTATTTCTTTAATGAGTAAATTTAAATATTAACAATGTTGCATTATTTTCGACAGCTGAGAAAACACTTACAGCCTGAACTGCTAAATCGTGTAGATGAAGTTATGATCTTTGATCCTCTAACACATGAGAAACTAAGGGTGGTTGCTAGGCTGCAGATGAAGGATGTGGCTAAACCTCTTGCTGAGAGGGGTATTACCTTGGCAGTCGCTGATGCTGCTCTAGATTATGTCTTAGCGGAGAGCTACGATCCAACAAGTAAATCTGGTGCCTTAATTTTATCCCTTCTTAAAATTTATTCCTTCTTCAACGGTATAAACTTTATTGTAAGTGTATGTACTAACTTCTAACTTCTTTCCCGTCATTAGGTTTATGGTGCAAGGCCTCTCAGGAGGTGGTTAGAAAAGAAGGTGGTTACAGAATTGTCAAAAATGCTCATTAAGGAGGAGATTGACAAGAACTCCACTGTCTTTATTGATTTCGGGATGGATGGCCTTGCTTATCGCGTTGAGAAAACTGGGGGAATGGTGGATGCTGCAACTGGACAGAGGGCAGAGGTCCTGATTCAAGTCCCCAACGGACAAAAGAGCAATGCCACTACTCAAGCTGCAAAGAAGTTAGAAATTGAGGTATTCCCTATTTTCTCTCCTCTCTCACTCTTTTAATAGGTTACAGATTCACCGTGTTTGATTTTTTTTTTTTTTTGGGCATTTGATCTGGATAATCAAGCTCTTATAAGCAATTTTGTTGCATCGCTTTGTCAGTTCATGTGTTGTGAAATGTGACAGAGGGAATCATTTCAGTGTCATGCGGTTGAGTTTCATAACCAAGAGGTGGAAATGTTGCCGGCTCATTCTTCAACAGGCACGCGGTCAGGGATTAGTAATCACTTTTCAATTCCAATTCTATTATGCCCTATATTTTTCTGTTGGTTTGATTTATATGATACAAAATATTGGTGCGCTTATGTTTTCTTTAATTATTAACTATTAGGAATCACCAGGTAATAGAATTTACTGAGTTTTATTGGCTTAGTTTTTGTACTTTAGCTATGCGTAAGTGTTATGTGTCCATCAAGATCTTTTTTGTGGCCTTGAGGGTTTATTCTAGATTCTTATGATATAATGTTATGGCATTCTATTTAAGGTATGAAATTTGGGGTCAATAATTAGTTGTTGTAATGTGTGACTGATATATAGAAGCCATGGAATTTGGGATTAAACTTGGGCTATGAAGCACAAGTGGTTGTTGCAACTTACTGCAGGGATAATAAAAATCCTTGAGTTGGGAACAAAGGCTACCGATTCACCATTTCACCATGATTTTGTGAGCGACTCACCATCTATATTTCTTCTTTTGCATATGGTATTTAGGTGGGATTTGGTTTTCGATATATATTTCAATTTTTTTGCTTACATTTTTTTTACTTCTTTTGGGTTGTTTTTTCTAAATTTGGATTTTTAATTCTAATTTTTGGAATTTCTAAATTGAGGTTTCAATTAGCTTTGTGAAATCTAGACCTTTAAATGAGTCTTTAAGTGTTTATGCTATAATTTGTCGAGATGATTTCGTTCCCTATAATTTGTTAGGATCTTTATGTGACACTAGCTTTAGAATTAATTTCTTTTTTGGGCTGTCATAACTCTTGCAATAAAAGAATTCTACTTCTACCTTACCTTTAATTAATGATTTACATCCGATCTGAGGGGATTTCTTATCTATCCAAGAATTAGGAACTGAGATTGCAGAACTTGAGAAGAAGAGAGATGAACTTGAAGCAGAACTGAAAAAGGTTTTGGTTGCTTTTTCTAATGCCTATCTATTATATGCTTTTAGTCTTTCCATGCACGGATCTTTGGTTCACTCCTCCTTGTCTTTGAATTTGTGTTTTCATTCCTTATTATATTTTGTAACTGTTTTACCCTGGCTTTTACTTGTGGAGTTGGGATTATTATTATTGTTGCACCATGACTTTTACATGTGTAGCTGATGAGGGCTATGCATGACATCTGTTAGTATGCAAAACTTGTAATCACCCAATTCTATGTGGGCTATTATTACACTTGTGGCTGTTGATTATGTGCGGGCTTAGTGAATTACTTTCTTCCTTTACTAGCTCTATGTACCCTGAAACACTTAAAGGCTCATTTAAAGGTCTAAATACCCAATGCAAGCTTTCATATTTAAATTGGTTTAAGAGATATATATATATATATATATATATATATATATATATATATATATATATATTTGCACTCAAATGAGAATGAGCCTTAAAATGAGAAGATATCTGGACCATTTAAATCAACCGTTAGGATTAGACTAGATTCTTTTATTTTTCTCCCGATTTTTTATATTCCCGTAGTACTTATGGGACCATTCTCTCTTTCCTCTTAGAAAAATCAAACGTCTTGCAATGGAGCCTTTCGTCAGTTTGTTGGAAAAGAATGCCCTGGAAGAGGACGAAGAGGATGACATTGTTACAAAGTTTTTTTTTTTTTTGAAGGATGTTACAAATTAGTTTGGTATGAAGTAAACCTTCTCTTTTGTGAACCATTTGCTAAGCTAGGATACTTGTAATTTTTTTTAACATAAAACGGAGACAATTTTATTATTTAGGACTCAAGTCCATCATTAAGTTTGTTACAAAAGGGGTCACACCAAAAACTTACAACCCATCTATACACACAACAAACTTGTTTATCCATGAAGTTTCGCAAAATAATAGGTATTGACTCCATGCTACTCCAGCCGAAACTTGAAAAACCGCAAAATATAGTTGTTTTATCCAAACTGGAGAGCTTCAACTTCCAACTATCTTCATTCCTTCCTGGTGGAGACTTCAGTCTCCTTAGTAACATCAGCACTGTGACTTGCATGTAACTCCACAGTCCCACCTTCAGTGTCTCTATTCCCCATTGGTACATCAGCAGGGATTGAACCAAACATAATTTGAGTAGTACTAGCACTTAAAGCAGAAGTTGTTCCTGAGTTGGCCTCTAAATCGATGACTTGTGGAGGTTCTCCTTCAGTAGCAAAAGTTCTGACCAGACCTTGTAAATCACCATGGTACGCAACTCTAGTAAAAGGAGGAGCATCCGCGAAAACTTTATGCCCCACAGCCATAGAGATAGAGTAA

mRNA sequence

ATGACATCTAATCTTGAGGTGGAGCATTTCCTCTCTGGATTCATGGGCAAGAGCACCATGGAGGCAGCTCAAGAGATCGAAAGTTATGGCACAGATGAAGTTATGATCTTTGATCCTCTAACACATGAGAAACTAAGGGTGGTTGCTAGGCTGCAGATGAAGGATGTGGCTAAACCTCTTGCTGAGAGGGGTATTACCTTGGCAGTCGCTGATGCTGCTCTAGATTATGTCTTAGCGGAGAGCTACGATCCAACAAGTAAATCTGGTGCCTTAATTTTATCCCTTCTTAAAATTTATTCCTTCTTCAACGGTATAAACTTTATTGTTTATGGTGCAAGGCCTCTCAGGAGGTGGTTAGAAAAGAAGGTGGTTACAGAATTGTCAAAAATGCTCATTAAGGAGGAGATTGACAAGAACTCCACTGTCTTTATTGATTTCGGGATGGATGGCCTTGCTTATCGCGTTGAGAAAACTGGGGGAATGGTGGATGCTGCAACTGGACAGAGGGCAGAGGTCCTGATTCAAGTCCCCAACGGACAAAAGAGCAATGCCACTACTCAAGCTGCAAAGAAGTTAGAAATTGAGGAATCACCAGAAGTTGTTCCTGAGTTGGCCTCTAAATCGATGACTTGTGGAGGTTCTCCTTCAGTAGCAAAAGTTCTGACCAGACCTTGTAAATCACCATGGTACGCAACTCTAGTAAAAGGAGGAGCATCCGCGAAAACTTTATGCCCCACAGCCATAGAGATAGAGTAA

Coding sequence (CDS)

ATGACATCTAATCTTGAGGTGGAGCATTTCCTCTCTGGATTCATGGGCAAGAGCACCATGGAGGCAGCTCAAGAGATCGAAAGTTATGGCACAGATGAAGTTATGATCTTTGATCCTCTAACACATGAGAAACTAAGGGTGGTTGCTAGGCTGCAGATGAAGGATGTGGCTAAACCTCTTGCTGAGAGGGGTATTACCTTGGCAGTCGCTGATGCTGCTCTAGATTATGTCTTAGCGGAGAGCTACGATCCAACAAGTAAATCTGGTGCCTTAATTTTATCCCTTCTTAAAATTTATTCCTTCTTCAACGGTATAAACTTTATTGTTTATGGTGCAAGGCCTCTCAGGAGGTGGTTAGAAAAGAAGGTGGTTACAGAATTGTCAAAAATGCTCATTAAGGAGGAGATTGACAAGAACTCCACTGTCTTTATTGATTTCGGGATGGATGGCCTTGCTTATCGCGTTGAGAAAACTGGGGGAATGGTGGATGCTGCAACTGGACAGAGGGCAGAGGTCCTGATTCAAGTCCCCAACGGACAAAAGAGCAATGCCACTACTCAAGCTGCAAAGAAGTTAGAAATTGAGGAATCACCAGAAGTTGTTCCTGAGTTGGCCTCTAAATCGATGACTTGTGGAGGTTCTCCTTCAGTAGCAAAAGTTCTGACCAGACCTTGTAAATCACCATGGTACGCAACTCTAGTAAAAGGAGGAGCATCCGCGAAAACTTTATGCCCCACAGCCATAGAGATAGAGTAA

Protein sequence

MTSNLEVEHFLSGFMGKSTMEAAQEIESYGTDEVMIFDPLTHEKLRVVARLQMKDVAKPLAERGITLAVADAALDYVLAESYDPTSKSGALILSLLKIYSFFNGINFIVYGARPLRRWLEKKVVTELSKMLIKEEIDKNSTVFIDFGMDGLAYRVEKTGGMVDAATGQRAEVLIQVPNGQKSNATTQAAKKLEIEESPEVVPELASKSMTCGGSPSVAKVLTRPCKSPWYATLVKGGASAKTLCPTAIEIE

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo22897.1	Spo22897.1	mRNA

Homology

[Close]

BLAST of Spo22897.1 vs. NCBI nr
Match: gi|902196130|gb|KNA13045.1| (hypothetical protein SOVF_120460 [Spinacia oleracea])

HSP 1 Score: 253.8 bits (647), Expect = 3.100e-64
Identity = 145/208 (69.71%), Postives = 158/208 (75.96%), Query Frame = 1


		  
Query: 1   MTSNLEVEHFLSGFMGKSTMEAAQ-----EIESY-------GTDEVMIFDPLTHEKLRVV 60
           MTSNL  E+ LSG MGKSTME A+     E+  +         DEV+IFDPL+HE+LR+V
Sbjct: 709 MTSNLGAEYLLSGLMGKSTMETARDKVMAEVRRHFKPELLNRLDEVVIFDPLSHEQLRLV 768

Query: 61  ARLQMKDVAKPLAERGITLAVADAALDYVLAESYDPTSKSGALILSLLKIYSFFNGINFI 120
           ARLQMKDVAK LAERGI LAV DAALDYVLAESYDP                        
Sbjct: 769 ARLQMKDVAKRLAERGIALAVTDAALDYVLAESYDP------------------------ 828

Query: 121 VYGARPLRRWLEKKVVTELSKMLIKEEIDKNSTVFIDFGMDGLAYRVEKTGGMVDAATGQ 180
           VYGARP+RRWLEKKVVTELSKMLIKEEID+NSTVFID GMDGL+YRVE+TGGMVDAATGQ
Sbjct: 829 VYGARPIRRWLEKKVVTELSKMLIKEEIDENSTVFIDAGMDGLSYRVEQTGGMVDAATGQ 888

Query: 181 RAEVLIQVPNGQKSNATTQAAKKLEIEE 197
           RAEVLIQVPNG KSNATTQAAKKL+IEE
Sbjct: 889 RAEVLIQVPNGPKSNATTQAAKKLKIEE 892

[Close]

BLAST of Spo22897.1 vs. NCBI nr
Match: gi|731366669|ref|XP_010695194.1| (PREDICTED: chaperone protein ClpB1 isoform X1 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 219.9 bits (559), Expect = 4.900e-54
Identity = 126/208 (60.58%), Postives = 150/208 (72.12%), Query Frame = 1


		  
Query: 1   MTSNLEVEHFLSGFMGKSTMEAAQE--IESYGT----------DEVMIFDPLTHEKLRVV 60
           MTSNL  EH LSG +GKSTM+AA+E  +E              DE+++FDPL+HE+L  V
Sbjct: 717 MTSNLGAEHLLSGLVGKSTMDAAREKVMEQVRRHFKPELLNRLDEIVVFDPLSHEQLMRV 776

Query: 61  ARLQMKDVAKPLAERGITLAVADAALDYVLAESYDPTSKSGALILSLLKIYSFFNGINFI 120
           ARLQMKDVAK LAERGI LAV DAALDYVL ESYDP                        
Sbjct: 777 ARLQMKDVAKRLAERGIALAVTDAALDYVLTESYDP------------------------ 836

Query: 121 VYGARPLRRWLEKKVVTELSKMLIKEEIDKNSTVFIDFGMDGLAYRVEKTGGMVDAATGQ 180
           VYGARP+RRWLEKKVVTE+SKMLI+EEID+NSTV+I+ G++GL YRVEK GGMV+AATGQ
Sbjct: 837 VYGARPIRRWLEKKVVTEMSKMLIREEIDENSTVYIEAGIEGLVYRVEKNGGMVNAATGQ 896

Query: 181 RAEVLIQVPNGQKSNATTQAAKKLEIEE 197
           +A+VLIQ+PNG  S   TQAAKK++I+E
Sbjct: 897 KADVLIQLPNGPGS-GLTQAAKKIKIQE 899

[Close]

BLAST of Spo22897.1 vs. NCBI nr
Match: gi|702496407|ref|XP_010037232.1| (PREDICTED: chaperone protein ClpB1 [Eucalyptus grandis])

HSP 1 Score: 212.2 bits (539), Expect = 1.000e-51
Identity = 122/210 (58.10%), Postives = 149/210 (70.95%), Query Frame = 1


		  
Query: 1   MTSNLEVEHFLSGFMGKSTMEAA-----QEIESY-------GTDEVMIFDPLTHEKLRVV 60
           MTSNL  EH LSG MGK TM+ A     QE+  +         DE+++FDPL+HE+LR V
Sbjct: 716 MTSNLGAEHLLSGLMGKCTMQVARDRVMQEVRRHFRPELLNRLDEIVVFDPLSHEQLRKV 775

Query: 61  ARLQMKDVAKPLAERGITLAVADAALDYVLAESYDPTSKSGALILSLLKIYSFFNGINFI 120
           ARLQMKDVA  LAERGI LAV DAALD+VLAESYDP                        
Sbjct: 776 ARLQMKDVASRLAERGIALAVTDAALDFVLAESYDP------------------------ 835

Query: 121 VYGARPLRRWLEKKVVTELSKMLIKEEIDKNSTVFIDFGMDG--LAYRVEKTGGMVDAAT 180
           VYGARP+RRWLEKKVVTELS+ML++EEID+NSTV+ID G +G  LAYRVEK GG V+AAT
Sbjct: 836 VYGARPIRRWLEKKVVTELSRMLLREEIDENSTVYIDAGPNGQDLAYRVEKNGGFVNAAT 895

Query: 181 GQRAEVLIQVPNGQKSNATTQAAKKLEIEE 197
           GQ++++LI++PNG +S+A  QA KK++IEE
Sbjct: 896 GQKSDLLIEIPNGPRSDA-AQAVKKMKIEE 900

[Close]

BLAST of Spo22897.1 vs. NCBI nr
Match: gi|526117643|ref|NP_001267822.1| (heat shock protein 101 [Vitis vinifera])

HSP 1 Score: 210.7 bits (535), Expect = 3.000e-51
Identity = 123/218 (56.42%), Postives = 152/218 (69.72%), Query Frame = 1


		  
Query: 1   MTSNLEVEHFLSGFMGKSTMEAA-----QEIESY-------GTDEVMIFDPLTHEKLRVV 60
           MTSNL  EH LSG +GK TM+ A     QE+  +         DE+++FDPL+H++LR V
Sbjct: 717 MTSNLGAEHLLSGLVGKCTMQDARDRVMQEVRRHFRPELLNRLDEIVVFDPLSHDQLRKV 776

Query: 61  ARLQMKDVAKPLAERGITLAVADAALDYVLAESYDPTSKSGALILSLLKIYSFFNGINFI 120
           ARLQMKDVA  LAERGI LAV DAALD VLAESYDP                        
Sbjct: 777 ARLQMKDVASRLAERGIALAVTDAALDVVLAESYDP------------------------ 836

Query: 121 VYGARPLRRWLEKKVVTELSKMLIKEEIDKNSTVFIDFGMD--GLAYRVEKTGGMVDAAT 180
           VYGARP+RRWLEKKVVTELSKMLI+EEID+NSTV+ID G+   GLAYRVE  GG+V+A+T
Sbjct: 837 VYGARPIRRWLEKKVVTELSKMLIREEIDENSTVYIDAGVSGKGLAYRVENNGGLVNAST 896

Query: 181 GQRAEVLIQVPNGQKSNATTQAAKKLEIEESPEVVPEL 205
           GQ+++VLI++PNGQ+S+A  QA KK++IEE  +   E+
Sbjct: 897 GQKSDVLIRIPNGQRSDA-AQAVKKMKIEEIEDEEDEM 909

[Close]

BLAST of Spo22897.1 vs. NCBI nr
Match: gi|254952729|gb|ACT97165.1| (heat shock protein [Vitis labrusca x Vitis vinifera])

HSP 1 Score: 210.7 bits (535), Expect = 3.000e-51
Identity = 123/218 (56.42%), Postives = 152/218 (69.72%), Query Frame = 1


		  
Query: 1   MTSNLEVEHFLSGFMGKSTMEAA-----QEIESY-------GTDEVMIFDPLTHEKLRVV 60
           MTSNL  EH LSG +GK TM+ A     QE+  +         DE+++FDPL+H++LR V
Sbjct: 717 MTSNLGAEHLLSGLVGKCTMQDARDRVMQEVRRHFRPELLNRLDEIVVFDPLSHDQLRKV 776

Query: 61  ARLQMKDVAKPLAERGITLAVADAALDYVLAESYDPTSKSGALILSLLKIYSFFNGINFI 120
           ARLQMKDVA  LAERGI LAV DAALD VLAESYDP                        
Sbjct: 777 ARLQMKDVASRLAERGIALAVTDAALDVVLAESYDP------------------------ 836

Query: 121 VYGARPLRRWLEKKVVTELSKMLIKEEIDKNSTVFIDFGMD--GLAYRVEKTGGMVDAAT 180
           VYGARP+RRWLEKKVVTELSKMLI+EEID+NSTV+ID G+   GLAYRVE  GG+V+A+T
Sbjct: 837 VYGARPIRRWLEKKVVTELSKMLIREEIDENSTVYIDAGVSGKGLAYRVENNGGLVNAST 896

Query: 181 GQRAEVLIQVPNGQKSNATTQAAKKLEIEESPEVVPEL 205
           GQ+++VLI++PNGQ+S+A  QA KK++IEE  +   E+
Sbjct: 897 GQKSDVLIRIPNGQRSDA-AQAVKKMKIEEIEDEEDEM 909

[Close]

BLAST of Spo22897.1 vs. UniProtKB/TrEMBL
Match: A0A0K9R0K3_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_120460 PE=3 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 2.100e-64
Identity = 145/208 (69.71%), Postives = 158/208 (75.96%), Query Frame = 1


		  
Query: 1   MTSNLEVEHFLSGFMGKSTMEAAQ-----EIESY-------GTDEVMIFDPLTHEKLRVV 60
           MTSNL  E+ LSG MGKSTME A+     E+  +         DEV+IFDPL+HE+LR+V
Sbjct: 709 MTSNLGAEYLLSGLMGKSTMETARDKVMAEVRRHFKPELLNRLDEVVIFDPLSHEQLRLV 768

Query: 61  ARLQMKDVAKPLAERGITLAVADAALDYVLAESYDPTSKSGALILSLLKIYSFFNGINFI 120
           ARLQMKDVAK LAERGI LAV DAALDYVLAESYDP                        
Sbjct: 769 ARLQMKDVAKRLAERGIALAVTDAALDYVLAESYDP------------------------ 828

Query: 121 VYGARPLRRWLEKKVVTELSKMLIKEEIDKNSTVFIDFGMDGLAYRVEKTGGMVDAATGQ 180
           VYGARP+RRWLEKKVVTELSKMLIKEEID+NSTVFID GMDGL+YRVE+TGGMVDAATGQ
Sbjct: 829 VYGARPIRRWLEKKVVTELSKMLIKEEIDENSTVFIDAGMDGLSYRVEQTGGMVDAATGQ 888

Query: 181 RAEVLIQVPNGQKSNATTQAAKKLEIEE 197
           RAEVLIQVPNG KSNATTQAAKKL+IEE
Sbjct: 889 RAEVLIQVPNGPKSNATTQAAKKLKIEE 892

[Close]

BLAST of Spo22897.1 vs. UniProtKB/TrEMBL
Match: A0A0J8E3S6_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_5g123390 PE=3 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 3.400e-54
Identity = 126/208 (60.58%), Postives = 150/208 (72.12%), Query Frame = 1


		  
Query: 1   MTSNLEVEHFLSGFMGKSTMEAAQE--IESYGT----------DEVMIFDPLTHEKLRVV 60
           MTSNL  EH LSG +GKSTM+AA+E  +E              DE+++FDPL+HE+L  V
Sbjct: 717 MTSNLGAEHLLSGLVGKSTMDAAREKVMEQVRRHFKPELLNRLDEIVVFDPLSHEQLMRV 776

Query: 61  ARLQMKDVAKPLAERGITLAVADAALDYVLAESYDPTSKSGALILSLLKIYSFFNGINFI 120
           ARLQMKDVAK LAERGI LAV DAALDYVL ESYDP                        
Sbjct: 777 ARLQMKDVAKRLAERGIALAVTDAALDYVLTESYDP------------------------ 836

Query: 121 VYGARPLRRWLEKKVVTELSKMLIKEEIDKNSTVFIDFGMDGLAYRVEKTGGMVDAATGQ 180
           VYGARP+RRWLEKKVVTE+SKMLI+EEID+NSTV+I+ G++GL YRVEK GGMV+AATGQ
Sbjct: 837 VYGARPIRRWLEKKVVTEMSKMLIREEIDENSTVYIEAGIEGLVYRVEKNGGMVNAATGQ 896

Query: 181 RAEVLIQVPNGQKSNATTQAAKKLEIEE 197
           +A+VLIQ+PNG  S   TQAAKK++I+E
Sbjct: 897 KADVLIQLPNGPGS-GLTQAAKKIKIQE 899

[Close]

BLAST of Spo22897.1 vs. UniProtKB/TrEMBL
Match: A0A059A5G2_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_K02521 PE=3 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 7.100e-52
Identity = 122/210 (58.10%), Postives = 149/210 (70.95%), Query Frame = 1


		  
Query: 1   MTSNLEVEHFLSGFMGKSTMEAA-----QEIESY-------GTDEVMIFDPLTHEKLRVV 60
           MTSNL  EH LSG MGK TM+ A     QE+  +         DE+++FDPL+HE+LR V
Sbjct: 716 MTSNLGAEHLLSGLMGKCTMQVARDRVMQEVRRHFRPELLNRLDEIVVFDPLSHEQLRKV 775

Query: 61  ARLQMKDVAKPLAERGITLAVADAALDYVLAESYDPTSKSGALILSLLKIYSFFNGINFI 120
           ARLQMKDVA  LAERGI LAV DAALD+VLAESYDP                        
Sbjct: 776 ARLQMKDVASRLAERGIALAVTDAALDFVLAESYDP------------------------ 835

Query: 121 VYGARPLRRWLEKKVVTELSKMLIKEEIDKNSTVFIDFGMDG--LAYRVEKTGGMVDAAT 180
           VYGARP+RRWLEKKVVTELS+ML++EEID+NSTV+ID G +G  LAYRVEK GG V+AAT
Sbjct: 836 VYGARPIRRWLEKKVVTELSRMLLREEIDENSTVYIDAGPNGQDLAYRVEKNGGFVNAAT 895

Query: 181 GQRAEVLIQVPNGQKSNATTQAAKKLEIEE 197
           GQ++++LI++PNG +S+A  QA KK++IEE
Sbjct: 896 GQKSDLLIEIPNGPRSDA-AQAVKKMKIEE 900

[Close]

BLAST of Spo22897.1 vs. UniProtKB/TrEMBL
Match: C7FB07_9ROSI (Heat shock protein OS=Vitis labrusca x Vitis vinifera PE=2 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 2.100e-51
Identity = 123/218 (56.42%), Postives = 152/218 (69.72%), Query Frame = 1


		  
Query: 1   MTSNLEVEHFLSGFMGKSTMEAA-----QEIESY-------GTDEVMIFDPLTHEKLRVV 60
           MTSNL  EH LSG +GK TM+ A     QE+  +         DE+++FDPL+H++LR V
Sbjct: 717 MTSNLGAEHLLSGLVGKCTMQDARDRVMQEVRRHFRPELLNRLDEIVVFDPLSHDQLRKV 776

Query: 61  ARLQMKDVAKPLAERGITLAVADAALDYVLAESYDPTSKSGALILSLLKIYSFFNGINFI 120
           ARLQMKDVA  LAERGI LAV DAALD VLAESYDP                        
Sbjct: 777 ARLQMKDVASRLAERGIALAVTDAALDVVLAESYDP------------------------ 836

Query: 121 VYGARPLRRWLEKKVVTELSKMLIKEEIDKNSTVFIDFGMD--GLAYRVEKTGGMVDAAT 180
           VYGARP+RRWLEKKVVTELSKMLI+EEID+NSTV+ID G+   GLAYRVE  GG+V+A+T
Sbjct: 837 VYGARPIRRWLEKKVVTELSKMLIREEIDENSTVYIDAGVSGKGLAYRVENNGGLVNAST 896

Query: 181 GQRAEVLIQVPNGQKSNATTQAAKKLEIEESPEVVPEL 205
           GQ+++VLI++PNGQ+S+A  QA KK++IEE  +   E+
Sbjct: 897 GQKSDVLIRIPNGQRSDA-AQAVKKMKIEEIEDEEDEM 909

[Close]

BLAST of Spo22897.1 vs. UniProtKB/TrEMBL
Match: Q3L1D0_VITVI (Heat shock protein 101 OS=Vitis vinifera GN=hsp101 PE=2 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 2.100e-51
Identity = 123/218 (56.42%), Postives = 152/218 (69.72%), Query Frame = 1


		  
Query: 1   MTSNLEVEHFLSGFMGKSTMEAA-----QEIESY-------GTDEVMIFDPLTHEKLRVV 60
           MTSNL  EH LSG +GK TM+ A     QE+  +         DE+++FDPL+H++LR V
Sbjct: 717 MTSNLGAEHLLSGLVGKCTMQDARDRVMQEVRRHFRPELLNRLDEIVVFDPLSHDQLRKV 776

Query: 61  ARLQMKDVAKPLAERGITLAVADAALDYVLAESYDPTSKSGALILSLLKIYSFFNGINFI 120
           ARLQMKDVA  LAERGI LAV DAALD VLAESYDP                        
Sbjct: 777 ARLQMKDVASRLAERGIALAVTDAALDVVLAESYDP------------------------ 836

Query: 121 VYGARPLRRWLEKKVVTELSKMLIKEEIDKNSTVFIDFGMD--GLAYRVEKTGGMVDAAT 180
           VYGARP+RRWLEKKVVTELSKMLI+EEID+NSTV+ID G+   GLAYRVE  GG+V+A+T
Sbjct: 837 VYGARPIRRWLEKKVVTELSKMLIREEIDENSTVYIDAGVSGKGLAYRVENNGGLVNAST 896

Query: 181 GQRAEVLIQVPNGQKSNATTQAAKKLEIEESPEVVPEL 205
           GQ+++VLI++PNGQ+S+A  QA KK++IEE  +   E+
Sbjct: 897 GQKSDVLIRIPNGQRSDA-AQAVKKMKIEEIEDEEDEM 909

[Close]

BLAST of Spo22897.1 vs. ExPASy Swiss-Prot
Match: CLPB1_ARATH (Chaperone protein ClpB1 OS=Arabidopsis thaliana GN=CLPB1 PE=1 SV=2)

HSP 1 Score: 199.5 bits (506), Expect = 4.300e-50
Identity = 112/208 (53.85%), Postives = 141/208 (67.79%), Query Frame = 1


		  
Query: 1   MTSNLEVEHFLSGFMGKSTMEAAQ-----EIESY-------GTDEVMIFDPLTHEKLRVV 60
           MTSNL  EH L+G  GK TME A+     E+  +         DE+++FDPL+H++LR V
Sbjct: 717 MTSNLGAEHLLAGLTGKVTMEVARDCVMREVRKHFRPELLNRLDEIVVFDPLSHDQLRKV 776

Query: 61  ARLQMKDVAKPLAERGITLAVADAALDYVLAESYDPTSKSGALILSLLKIYSFFNGINFI 120
           ARLQMKDVA  LAERG+ LAV DAALDY+LAESYDP                        
Sbjct: 777 ARLQMKDVAVRLAERGVALAVTDAALDYILAESYDP------------------------ 836

Query: 121 VYGARPLRRWLEKKVVTELSKMLIKEEIDKNSTVFIDFGMDGLAYRVEKTGGMVDAATGQ 180
           VYGARP+RRW+EKKVVTELSKM+++EEID+NSTV+ID G   L YRVE +GG+VDA+TG+
Sbjct: 837 VYGARPIRRWMEKKVVTELSKMVVREEIDENSTVYIDAGAGDLVYRVE-SGGLVDASTGK 896

Query: 181 RAEVLIQVPNGQKSNATTQAAKKLEIEE 197
           +++VLI + NG K +   QA KK+ IEE
Sbjct: 897 KSDVLIHIANGPKRSDAAQAVKKMRIEE 899

[Close]

BLAST of Spo22897.1 vs. ExPASy Swiss-Prot
Match: CLPB1_ORYSJ (Chaperone protein ClpB1 OS=Oryza sativa subsp. japonica GN=CLPB1 PE=2 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 4.900e-46
Identity = 110/218 (50.46%), Postives = 143/218 (65.60%), Query Frame = 1


		  
Query: 1   MTSNLEVEHFLSGFMGKSTMEAA-----QEIESY-------GTDEVMIFDPLTHEKLRVV 60
           MTSNL  EH L+G +GK++M+ A     QE+  +         DE++IFDPL+HE+LR V
Sbjct: 718 MTSNLGAEHLLAGMVGKNSMKVARDLVMQEVRRHFRPELLNRLDEIVIFDPLSHEQLRKV 777

Query: 61  ARLQMKDVAKPLAERGITLAVADAALDYVLAESYDPTSKSGALILSLLKIYSFFNGINFI 120
           ARLQMKDVA  LAERG+ LAV DAALD +L+ SYDP                        
Sbjct: 778 ARLQMKDVAVRLAERGVALAVTDAALDVILSLSYDP------------------------ 837

Query: 121 VYGARPLRRWLEKKVVTELSKMLIKEEIDKNSTVFIDFG--MDGLAYRVEKTGGMVDAAT 180
           VYGARP+RRW+EK+VVT+LSKMLI+EEID+N TV+ID     D LAYRV+  GG+V+A T
Sbjct: 838 VYGARPIRRWIEKRVVTQLSKMLIQEEIDENCTVYIDAAPHKDELAYRVDNRGGLVNAET 897

Query: 181 GQRAEVLIQVPNGQKSNA-TTQAAKKLEIEESPEVVPE 204
           GQ++++LIQVPNG  + +   QA KK+ I E  + + E
Sbjct: 898 GQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDE 911

[Close]

BLAST of Spo22897.1 vs. ExPASy Swiss-Prot
Match: CLPB_BACTN (Chaperone protein ClpB OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482) GN=clpB PE=3 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 4.200e-13
Identity = 40/123 (32.52%), Postives = 68/123 (55.28%), Query Frame = 1


		  
Query: 32  DEVMIFDPLTHEKLRVVARLQMKDVAKPLAERGITLAVADAALDYVLAESYDPTSKSGAL 91
           DE ++F PLT  ++R +  LQ+K V K LAE G+ L + DAAL+++    YDP       
Sbjct: 763 DETIMFLPLTETEIRQIVLLQIKGVQKMLAENGVELEMTDAALNFLSQVGYDPE------ 822

Query: 92  ILSLLKIYSFFNGINFIVYGARPLRRWLEKKVVTELSKMLIKEEIDKNSTVFIDFGMDGL 151
                             +GARP++R +++ ++ +LSK L+ +E+D++  + +D   DGL
Sbjct: 823 ------------------FGARPVKRAIQRYLLNDLSKKLLSQEVDRSKAIIVDSNGDGL 861

Query: 152 AYR 155
            +R
Sbjct: 883 VFR 861

[Close]

BLAST of Spo22897.1 vs. ExPASy Swiss-Prot
Match: CLPB_MEIRU (Chaperone protein ClpB OS=Meiothermus ruber GN=clpB PE=3 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 6.700e-11
Identity = 37/127 (29.13%), Postives = 67/127 (52.76%), Query Frame = 1


		  
Query: 32  DEVMIFDPLTHEKLRVVARLQMKDVAKPLAERGITLAVADAALDYVLAESYDPTSKSGAL 91
           DE+++F PL  E++  + ++Q++ V K LAER ITL ++  ALD++    YDP       
Sbjct: 749 DEIVVFRPLAREQIAAIVQIQLEAVRKRLAERRITLELSQEALDFLAQRGYDP------- 808

Query: 92  ILSLLKIYSFFNGINFIVYGARPLRRWLEKKVVTELSKMLIKEEIDKNSTVFIDFGMDGL 151
                            V+GARPL+R +++++ T LS+ ++  E+   + +++  G  GL
Sbjct: 809 -----------------VFGARPLKRVIQRELETPLSRKILAGEVADGAHLYVGSGPLGL 851

Query: 152 AYRVEKT 159
            +    T
Sbjct: 869 TFEARPT 851

[Close]

BLAST of Spo22897.1 vs. ExPASy Swiss-Prot
Match: CLPB_GLOVI (Chaperone protein ClpB OS=Gloeobacter violaceus (strain PCC 7421) GN=clpB PE=3 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 8.800e-11
Identity = 37/123 (30.08%), Postives = 67/123 (54.47%), Query Frame = 1


		  
Query: 32  DEVMIFDPLTHEKLRVVARLQMKDVAKPLAERGITLAVADAALDYVLAESYDPTSKSGAL 91
           D+++IF  L  ++L  + +LQ+  + K LA+R ITL +++AA+DY++   YDP       
Sbjct: 766 DDIIIFRNLRRDQLAAIVKLQIARLEKRLADRKITLKLSEAAIDYIVEAGYDP------- 825

Query: 92  ILSLLKIYSFFNGINFIVYGARPLRRWLEKKVVTELSKMLIKEEIDKNSTVFIDFGMDGL 151
                            VYGARPL+R ++ ++V  L++ L+K + +   T+F+D   +  
Sbjct: 826 -----------------VYGARPLKRAIQNELVNPLARGLLKGDFNDGDTIFVDIENERP 864

Query: 152 AYR 155
            +R
Sbjct: 886 VFR 864

[Close]

BLAST of Spo22897.1 vs. TAIR (Arabidopsis)
Match: AT1G74310.1 (heat shock protein 101)

HSP 1 Score: 199.5 bits (506), Expect = 2.400e-51
Identity = 112/208 (53.85%), Postives = 141/208 (67.79%), Query Frame = 1


		  
Query: 1   MTSNLEVEHFLSGFMGKSTMEAAQ-----EIESY-------GTDEVMIFDPLTHEKLRVV 60
           MTSNL  EH L+G  GK TME A+     E+  +         DE+++FDPL+H++LR V
Sbjct: 717 MTSNLGAEHLLAGLTGKVTMEVARDCVMREVRKHFRPELLNRLDEIVVFDPLSHDQLRKV 776

Query: 61  ARLQMKDVAKPLAERGITLAVADAALDYVLAESYDPTSKSGALILSLLKIYSFFNGINFI 120
           ARLQMKDVA  LAERG+ LAV DAALDY+LAESYDP                        
Sbjct: 777 ARLQMKDVAVRLAERGVALAVTDAALDYILAESYDP------------------------ 836

Query: 121 VYGARPLRRWLEKKVVTELSKMLIKEEIDKNSTVFIDFGMDGLAYRVEKTGGMVDAATGQ 180
           VYGARP+RRW+EKKVVTELSKM+++EEID+NSTV+ID G   L YRVE +GG+VDA+TG+
Sbjct: 837 VYGARPIRRWMEKKVVTELSKMVVREEIDENSTVYIDAGAGDLVYRVE-SGGLVDASTGK 896

Query: 181 RAEVLIQVPNGQKSNATTQAAKKLEIEE 197
           +++VLI + NG K +   QA KK+ IEE
Sbjct: 897 KSDVLIHIANGPKRSDAAQAVKKMRIEE 899

[Close]

BLAST of Spo22897.1 vs. TAIR (Arabidopsis)
Match: AT5G15450.1 (casein lytic proteinase B3)

HSP 1 Score: 57.0 bits (136), Expect = 1.900e-8
Identity = 31/114 (27.19%), Postives = 58/114 (50.88%), Query Frame = 1


		  
Query: 32  DEVMIFDPLTHEKLRVVARLQMKDVAKPLAERGITLAVADAALDYVLAESYDPTSKSGAL 91
           DE ++F PL  E++  + RLQ+  V K +A+R + + + DAA+D + +  YDP       
Sbjct: 842 DEYIVFKPLDREQINRIVRLQLARVQKRIADRKMKINITDAAVDLLGSLGYDPN------ 901

Query: 92  ILSLLKIYSFFNGINFIVYGARPLRRWLEKKVVTELSKMLIKEEIDKNSTVFID 146
                             YGARP++R +++ +  EL+K +++ +  +   + ID
Sbjct: 902 ------------------YGARPVKRVIQQNIENELAKGILRGDFKEEDGILID 931

The following BLAST results are available for this feature:

BLAST of Spo22897.1 vs. NCBI nr
BLAST of Spo22897.1 vs. UniProtKB/TrEMBL
BLAST of Spo22897.1 vs. ExPASy Swiss-Prot
BLAST of Spo22897.1 vs. TAIR (Arabidopsis)

BLAST of Spo22897.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902196130\|gb\|KNA13045.1\|	3.1e-64	69.7	hypothetical protein SOVF_1204...	[more]
gi\|731366669\|ref\|XP_010695194.1\|	4.9e-54	60.5	PREDICTED: chaperone protein C...	[more]
gi\|702496407\|ref\|XP_010037232.1\|	1.0e-51	58.1	PREDICTED: chaperone protein C...	[more]
gi\|526117643\|ref\|NP_001267822.1\|	3.0e-51	56.4	heat shock protein 101 [Vitis ...	[more]
gi\|254952729\|gb\|ACT97165.1\|	3.0e-51	56.4	heat shock protein [Vitis labr...	[more]

BLAST of Spo22897.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9R0K3_SPIOL	2.1e-64	69.7	Uncharacterized protein OS=Spi...	[more]
A0A0J8E3S6_BETVU	3.4e-54	60.5	Uncharacterized protein OS=Bet...	[more]
A0A059A5G2_EUCGR	7.1e-52	58.1	Uncharacterized protein OS=Euc...	[more]
C7FB07_9ROSI	2.1e-51	56.4	Heat shock protein OS=Vitis la...	[more]
Q3L1D0_VITVI	2.1e-51	56.4	Heat shock protein 101 OS=Viti...	[more]

BLAST of Spo22897.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
CLPB1_ARATH	4.3e-50	53.8	Chaperone protein ClpB1 OS=Ara...	[more]
CLPB1_ORYSJ	4.9e-46	50.4	Chaperone protein ClpB1 OS=Ory...	[more]
CLPB_BACTN	4.2e-13	32.5	Chaperone protein ClpB OS=Bact...	[more]
CLPB_MEIRU	6.7e-11	29.1	Chaperone protein ClpB OS=Meio...	[more]
CLPB_GLOVI	8.8e-11	30.0	Chaperone protein ClpB OS=Gloe...	[more]

BLAST of Spo22897.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 2


Match Name	E-value	Identity	Description
AT1G74310.1	2.4e-51	53.8	heat shock protein 101	[more]
AT5G15450.1	1.9e-8	27.1	casein lytic proteinase B3	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019489	Clp ATPase, C-terminal	PFAM	PF10431	ClpB_D2-small	coord: 40..85 score: 1.4E-6coord: 109..142 score: 1.
IPR019489	Clp ATPase, C-terminal	SMART	SM01086	ClpB_D2_small_2	coord: 40..153 score: 4.5
None	No IPR available	GENE3D	1.10.8.60		coord: 109..157 score: 5.2E-10coord: 40..85 score: 2.
None	No IPR available	PANTHER	PTHR11638	ATP-DEPENDENT CLP PROTEASE	coord: 1..84 score: 2.0E-55coord: 109..172 score: 2.0
None	No IPR available	PANTHER	PTHR11638:SF116	SUBFAMILY NOT NAMED	coord: 109..172 score: 2.0E-55coord: 1..84 score: 2.0

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006886	intracellular protein transport
biological_process	GO:0016192	vesicle-mediated transport
cellular_component	GO:0030132	clathrin coat of coated pit
cellular_component	GO:0030130	clathrin coat of trans-Golgi network vesicle
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005515	protein binding
molecular_function	GO:0005488	binding
molecular_function	GO:0005198	structural molecule activity
molecular_function	GO:0003676	nucleic acid binding

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo22896	0.80	Barchart \| Table
Spo04492	0.67	Barchart \| Table
Spo13095	0.67	Barchart \| Table
Spo05407	0.66	Barchart \| Table
Spo18997	0.66	Barchart \| Table