Spo23654 (gene)

Overview
NameSpo23654
Typegene
OrganismSpinacia oleracea (Spinach)
DescriptionCalcium binding protein
Locationchr2 : 52143915 .. 52144436 (+)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGACCACCACTACTGAAGAAAACAGCAACAAATCCTCATCACACTCTCTCTCCTCCGTTTACCTCTCTGACACAGCGGAGGTCAAGAAAGTCTTCGACCGATTCGACGTCAACGGCGACGGAAAAATCTCCGCTGAAGAACTCATCGGCGTTATGAAGGCGCTCGGATCGGAAACCTCACCGGAAGAAGTTGCGCAGATGATGGAGGAGATGGATACCGATCGCGATGGCTTCATTAACCTCGAGGAATTCGCCGATTTCTGCTCTCGCAAAGTTGAAGGTGATGAATCCGGGTCGCAGGAGCTTCATGACGCGTTCAAGATGTATGACCAGGATGAAAATGGCTTGATCTCTGCTGCTGAGCTACATCTCGTTCTGAGTCGACTCGGTGAGGGTTGCTCTGTTGAAGATTGCTCGAAGATGATTCAGTCTGTTGATGCTGATGGCGATGGTAATGTTAGCTACGAGGAGTTTCGTAAGATGATGACCAAAGGTAAATCGACGTCGTCGTCAAATTAG

mRNA sequence

ATGGCGACCACCACTACTGAAGAAAACAGCAACAAATCCTCATCACACTCTCTCTCCTCCGTTTACCTCTCTGACACAGCGGAGGTCAAGAAAGTCTTCGACCGATTCGACGTCAACGGCGACGGAAAAATCTCCGCTGAAGAACTCATCGGCGTTATGAAGGCGCTCGGATCGGAAACCTCACCGGAAGAAGTTGCGCAGATGATGGAGGAGATGGATACCGATCGCGATGGCTTCATTAACCTCGAGGAATTCGCCGATTTCTGCTCTCGCAAAGTTGAAGGTGATGAATCCGGGTCGCAGGAGCTTCATGACGCGTTCAAGATGTATGACCAGGATGAAAATGGCTTGATCTCTGCTGCTGAGCTACATCTCGTTCTGAGTCGACTCGGTGAGGGTTGCTCTGTTGAAGATTGCTCGAAGATGATTCAGTCTGTTGATGCTGATGGCGATGGTAATGTTAGCTACGAGGAGTTTCGTAAGATGATGACCAAAGGTAAATCGACGTCGTCGTCAAATTAG

Coding sequence (CDS)

ATGGCGACCACCACTACTGAAGAAAACAGCAACAAATCCTCATCACACTCTCTCTCCTCCGTTTACCTCTCTGACACAGCGGAGGTCAAGAAAGTCTTCGACCGATTCGACGTCAACGGCGACGGAAAAATCTCCGCTGAAGAACTCATCGGCGTTATGAAGGCGCTCGGATCGGAAACCTCACCGGAAGAAGTTGCGCAGATGATGGAGGAGATGGATACCGATCGCGATGGCTTCATTAACCTCGAGGAATTCGCCGATTTCTGCTCTCGCAAAGTTGAAGGTGATGAATCCGGGTCGCAGGAGCTTCATGACGCGTTCAAGATGTATGACCAGGATGAAAATGGCTTGATCTCTGCTGCTGAGCTACATCTCGTTCTGAGTCGACTCGGTGAGGGTTGCTCTGTTGAAGATTGCTCGAAGATGATTCAGTCTGTTGATGCTGATGGCGATGGTAATGTTAGCTACGAGGAGTTTCGTAAGATGATGACCAAAGGTAAATCGACGTCGTCGTCAAATTAG

Protein sequence

MATTTTEENSNKSSSHSLSSVYLSDTAEVKKVFDRFDVNGDGKISAEELIGVMKALGSETSPEEVAQMMEEMDTDRDGFINLEEFADFCSRKVEGDESGSQELHDAFKMYDQDENGLISAAELHLVLSRLGEGCSVEDCSKMIQSVDADGDGNVSYEEFRKMMTKGKSTSSSN
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo23654.1Spo23654.1mRNA


Homology
[Close]
BLAST of Spo23654.1 vs. NCBI nr
Match: gi|902185423|gb|KNA10488.1| (hypothetical protein SOVF_143520 [Spinacia oleracea])

HSP 1 Score: 330.9 bits (847), Expect = 1.400e-87
Identity = 173/173 (100.00%), Postives = 173/173 (100.00%), Query Frame = 1 

		  

Query: 1   MATTTTEENSNKSSSHSLSSVYLSDTAEVKKVFDRFDVNGDGKISAEELIGVMKALGSET 60
           MATTTTEENSNKSSSHSLSSVYLSDTAEVKKVFDRFDVNGDGKISAEELIGVMKALGSET
Sbjct: 1   MATTTTEENSNKSSSHSLSSVYLSDTAEVKKVFDRFDVNGDGKISAEELIGVMKALGSET 60

Query: 61  SPEEVAQMMEEMDTDRDGFINLEEFADFCSRKVEGDESGSQELHDAFKMYDQDENGLISA 120
           SPEEVAQMMEEMDTDRDGFINLEEFADFCSRKVEGDESGSQELHDAFKMYDQDENGLISA
Sbjct: 61  SPEEVAQMMEEMDTDRDGFINLEEFADFCSRKVEGDESGSQELHDAFKMYDQDENGLISA 120

Query: 121 AELHLVLSRLGEGCSVEDCSKMIQSVDADGDGNVSYEEFRKMMTKGKSTSSSN 174
           AELHLVLSRLGEGCSVEDCSKMIQSVDADGDGNVSYEEFRKMMTKGKSTSSSN
Sbjct: 121 AELHLVLSRLGEGCSVEDCSKMIQSVDADGDGNVSYEEFRKMMTKGKSTSSSN 173
[Close]
BLAST of Spo23654.1 vs. NCBI nr
Match: gi|731311858|ref|XP_010671432.1| (PREDICTED: calcium-binding allergen Ole e 8-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 271.6 bits (693), Expect = 9.800e-70
Identity = 139/169 (82.25%), Postives = 154/169 (91.12%), Query Frame = 1 

		  

Query: 5   TTEENSNKSSSHSLSSVYLSDTAEVKKVFDRFDVNGDGKISAEELIGVMKALGSETSPEE 64
           +TE NSN SSSHSLSSVYLSD AEVKKVFDRFD NGDGKISA EL  VMKALGSETSPEE
Sbjct: 4   STENNSNSSSSHSLSSVYLSDMAEVKKVFDRFDTNGDGKISATELKNVMKALGSETSPEE 63

Query: 65  VAQMMEEMDTDRDGFINLEEFADFCSRKVEGDESGSQELHDAFKMYDQDENGLISAAELH 124
           V QMMEEMD D+D +I+LEEFA+FCS+KVEGDE+GSQELHDAF+MYDQD+NGLISA ELH
Sbjct: 64  VKQMMEEMDIDKDSYISLEEFAEFCSKKVEGDENGSQELHDAFEMYDQDKNGLISATELH 123

Query: 125 LVLSRLGEGCSVEDCSKMIQSVDADGDGNVSYEEFRKMMTKGKSTSSSN 174
           LVLS+LGE C+V DC KMIQSVDADGDGNVS++EFRKMMT+GKS+SS N
Sbjct: 124 LVLSQLGEHCTVVDCEKMIQSVDADGDGNVSFDEFRKMMTRGKSSSSQN 172
[Close]
BLAST of Spo23654.1 vs. NCBI nr
Match: gi|661898388|emb|CDO98355.1| (unnamed protein product [Coffea canephora])

HSP 1 Score: 208.8 bits (530), Expect = 7.800e-51
Identity = 108/172 (62.79%), Postives = 134/172 (77.91%), Query Frame = 1 

		  

Query: 1   MATTTTEENSNKSSSHSLSSVYLSDTAEVKKVFDRFDVNGDGKISAEELIGVMKALGSET 60
           MATTTT   +N  S+ S  S YL D  EV+KVF+RFD N DGKIS+EEL GVMKALGS+T
Sbjct: 46  MATTTT---NNADSNSSKPSAYLQDADEVQKVFERFDANSDGKISSEELAGVMKALGSDT 105

Query: 61  SPEEVAQMMEEMDTDRDGFINLEEFADFCSR-----KVEGDESGSQELHDAFKMYDQDEN 120
           SP+E+++MM E+DTD+DGFINLEEF  FCS        +G + G +ELHDAF++YDQD +
Sbjct: 106 SPDEISRMMVEIDTDKDGFINLEEFTAFCSSNSLYGSADGGDGGVKELHDAFELYDQDHD 165

Query: 121 GLISAAELHLVLSRLGEGCSVEDCSKMIQSVDADGDGNVSYEEFRKMMTKGK 168
           G ISA ELHL+L+RLGE CSV+DC KMI+SVD+DGDG VS++EF+ MMT  K
Sbjct: 166 GFISATELHLILTRLGERCSVQDCEKMIKSVDSDGDGRVSFQEFKIMMTNSK 214
[Close]
BLAST of Spo23654.1 vs. NCBI nr
Match: gi|698502976|ref|XP_009797110.1| (PREDICTED: calcium-binding allergen Ole e 8-like [Nicotiana sylvestris])

HSP 1 Score: 205.3 bits (521), Expect = 8.600e-50
Identity = 104/164 (63.41%), Postives = 131/164 (79.88%), Query Frame = 1 

		  

Query: 5   TTEENSNKSSSHSLSSVYLSDTAEVKKVFDRFDVNGDGKISAEELIGVMKALGSETSPEE 64
           T   N+N ++S S  S+YL D  EV+KVF RFD NGDG ISA+EL GV+ ALGS+TSP+E
Sbjct: 4   TDNVNNNNNNSISKPSLYLQDMDEVQKVFKRFDTNGDGMISADELSGVINALGSDTSPDE 63

Query: 65  VAQMMEEMDTDRDGFINLEEFADFCSRKVEG-DESGSQELHDAFKMYDQDENGLISAAEL 124
           VA+MM+E+DTDRDG+INL+EFA+FC   V G  + G  EL +AF +YDQD NGLISAAEL
Sbjct: 64  VARMMDEIDTDRDGYINLDEFAEFCKGDVNGVGDGGVNELREAFDLYDQDSNGLISAAEL 123

Query: 125 HLVLSRLGEGCSVEDCSKMIQSVDADGDGNVSYEEFRKMMTKGK 168
           H +L+RLGE CSV+DC+KMI +VDADGDG V+++EF+KMMT  K
Sbjct: 124 HQILTRLGESCSVQDCTKMINTVDADGDGYVNFDEFKKMMTNNK 167
[Close]
BLAST of Spo23654.1 vs. NCBI nr
Match: gi|697175630|ref|XP_009596756.1| (PREDICTED: calcium-binding allergen Ole e 8-like [Nicotiana tomentosiformis])

HSP 1 Score: 204.5 bits (519), Expect = 1.500e-49
Identity = 104/163 (63.80%), Postives = 129/163 (79.14%), Query Frame = 1 

		  

Query: 6   TEENSNKSSSHSLSSVYLSDTAEVKKVFDRFDVNGDGKISAEELIGVMKALGSETSPEEV 65
           T   +N ++S S  S+YL D  EV+KVF RFD NGDG ISA+EL GV+ ALGS+TSP+EV
Sbjct: 4   TNNGNNNNNSVSKPSLYLQDMDEVQKVFKRFDTNGDGMISADELSGVINALGSDTSPDEV 63

Query: 66  AQMMEEMDTDRDGFINLEEFADFCSRKVEG-DESGSQELHDAFKMYDQDENGLISAAELH 125
           A+MM+E+DTDRDG+INL+EFA+FC   V G  + G  EL +AF +YDQD NGLISAAELH
Sbjct: 64  ARMMDEIDTDRDGYINLDEFAEFCKGDVNGVGDGGVNELREAFDLYDQDSNGLISAAELH 123

Query: 126 LVLSRLGEGCSVEDCSKMIQSVDADGDGNVSYEEFRKMMTKGK 168
            +L RLGE CSV+DC+KMI SVDADGDG V+++EF+KMMT  K
Sbjct: 124 QILIRLGESCSVQDCTKMISSVDADGDGYVNFDEFKKMMTNSK 166
[Close]
BLAST of Spo23654.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QUU9_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_143520 PE=4 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 9.500e-88
Identity = 173/173 (100.00%), Postives = 173/173 (100.00%), Query Frame = 1 

		  

Query: 1   MATTTTEENSNKSSSHSLSSVYLSDTAEVKKVFDRFDVNGDGKISAEELIGVMKALGSET 60
           MATTTTEENSNKSSSHSLSSVYLSDTAEVKKVFDRFDVNGDGKISAEELIGVMKALGSET
Sbjct: 1   MATTTTEENSNKSSSHSLSSVYLSDTAEVKKVFDRFDVNGDGKISAEELIGVMKALGSET 60

Query: 61  SPEEVAQMMEEMDTDRDGFINLEEFADFCSRKVEGDESGSQELHDAFKMYDQDENGLISA 120
           SPEEVAQMMEEMDTDRDGFINLEEFADFCSRKVEGDESGSQELHDAFKMYDQDENGLISA
Sbjct: 61  SPEEVAQMMEEMDTDRDGFINLEEFADFCSRKVEGDESGSQELHDAFKMYDQDENGLISA 120

Query: 121 AELHLVLSRLGEGCSVEDCSKMIQSVDADGDGNVSYEEFRKMMTKGKSTSSSN 174
           AELHLVLSRLGEGCSVEDCSKMIQSVDADGDGNVSYEEFRKMMTKGKSTSSSN
Sbjct: 121 AELHLVLSRLGEGCSVEDCSKMIQSVDADGDGNVSYEEFRKMMTKGKSTSSSN 173
[Close]
BLAST of Spo23654.1 vs. UniProtKB/TrEMBL
Match: A0A0J8D893_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_1g005460 PE=4 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 6.800e-70
Identity = 139/169 (82.25%), Postives = 154/169 (91.12%), Query Frame = 1 

		  

Query: 5   TTEENSNKSSSHSLSSVYLSDTAEVKKVFDRFDVNGDGKISAEELIGVMKALGSETSPEE 64
           +TE NSN SSSHSLSSVYLSD AEVKKVFDRFD NGDGKISA EL  VMKALGSETSPEE
Sbjct: 4   STENNSNSSSSHSLSSVYLSDMAEVKKVFDRFDTNGDGKISATELKNVMKALGSETSPEE 63

Query: 65  VAQMMEEMDTDRDGFINLEEFADFCSRKVEGDESGSQELHDAFKMYDQDENGLISAAELH 124
           V QMMEEMD D+D +I+LEEFA+FCS+KVEGDE+GSQELHDAF+MYDQD+NGLISA ELH
Sbjct: 64  VKQMMEEMDIDKDSYISLEEFAEFCSKKVEGDENGSQELHDAFEMYDQDKNGLISATELH 123

Query: 125 LVLSRLGEGCSVEDCSKMIQSVDADGDGNVSYEEFRKMMTKGKSTSSSN 174
           LVLS+LGE C+V DC KMIQSVDADGDGNVS++EFRKMMT+GKS+SS N
Sbjct: 124 LVLSQLGEHCTVVDCEKMIQSVDADGDGNVSFDEFRKMMTRGKSSSSQN 172
[Close]
BLAST of Spo23654.1 vs. UniProtKB/TrEMBL
Match: A0A068TPW5_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00022422001 PE=4 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 5.400e-51
Identity = 108/172 (62.79%), Postives = 134/172 (77.91%), Query Frame = 1 

		  

Query: 1   MATTTTEENSNKSSSHSLSSVYLSDTAEVKKVFDRFDVNGDGKISAEELIGVMKALGSET 60
           MATTTT   +N  S+ S  S YL D  EV+KVF+RFD N DGKIS+EEL GVMKALGS+T
Sbjct: 46  MATTTT---NNADSNSSKPSAYLQDADEVQKVFERFDANSDGKISSEELAGVMKALGSDT 105

Query: 61  SPEEVAQMMEEMDTDRDGFINLEEFADFCSR-----KVEGDESGSQELHDAFKMYDQDEN 120
           SP+E+++MM E+DTD+DGFINLEEF  FCS        +G + G +ELHDAF++YDQD +
Sbjct: 106 SPDEISRMMVEIDTDKDGFINLEEFTAFCSSNSLYGSADGGDGGVKELHDAFELYDQDHD 165

Query: 121 GLISAAELHLVLSRLGEGCSVEDCSKMIQSVDADGDGNVSYEEFRKMMTKGK 168
           G ISA ELHL+L+RLGE CSV+DC KMI+SVD+DGDG VS++EF+ MMT  K
Sbjct: 166 GFISATELHLILTRLGERCSVQDCEKMIKSVDSDGDGRVSFQEFKIMMTNSK 214
[Close]
BLAST of Spo23654.1 vs. UniProtKB/TrEMBL
Match: K4BM30_SOLLC (Uncharacterized protein OS=Solanum lycopersicum PE=4 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 4.000e-46
Identity = 102/168 (60.71%), Postives = 127/168 (75.60%), Query Frame = 1 

		  

Query: 1   MATTTTEENSNKSSSHSLSSVYLSDTAEVKKVFDRFDVNGDGKISAEELIGVMKALGSET 60
           MA T    N++ S      S+YL +  EV+KVF RFD NGDG IS EEL GV+ ALGS T
Sbjct: 1   MAATDNVNNNSVSKP----SLYLQNMDEVQKVFQRFDTNGDGMISGEELSGVISALGSGT 60

Query: 61  SPEEVAQMMEEMDTDRDGFINLEEFADFCSRKVEG-DESGSQELHDAFKMYDQDENGLIS 120
           SPEEV++MM E+D+DRDGFI+L+EFA+FC   +    + G +EL +AF +YDQD NGLIS
Sbjct: 61  SPEEVSRMMNEIDSDRDGFISLQEFAEFCKGDINSIGDGGVRELREAFDLYDQDSNGLIS 120

Query: 121 AAELHLVLSRLGEGCSVEDCSKMIQSVDADGDGNVSYEEFRKMMTKGK 168
           AAELH +L+RLGE CSV+DC+KMI SVD+DGDG VS+EEF+KMMT  K
Sbjct: 121 AAELHQILTRLGENCSVQDCTKMINSVDSDGDGYVSFEEFKKMMTTSK 164
[Close]
BLAST of Spo23654.1 vs. UniProtKB/TrEMBL
Match: A0A0V0HFM4_SOLCH (Putative calcium-binding allergen Ole e 8-like OS=Solanum chacoense PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 5.300e-46
Identity = 102/168 (60.71%), Postives = 127/168 (75.60%), Query Frame = 1 

		  

Query: 1   MATTTTEENSNKSSSHSLSSVYLSDTAEVKKVFDRFDVNGDGKISAEELIGVMKALGSET 60
           MA T    N++ S      S+YL +  EV+KVF RFD NGDG IS +EL GV+ ALGS T
Sbjct: 1   MAATDNVNNNSVSKP----SLYLQNMDEVQKVFQRFDTNGDGMISGDELSGVISALGSGT 60

Query: 61  SPEEVAQMMEEMDTDRDGFINLEEFADFCSRKVEG-DESGSQELHDAFKMYDQDENGLIS 120
           SPEEVA+MM+E+D DRDGFI+L+EFA+FC   +    + G +EL +AF +YDQD NGLIS
Sbjct: 61  SPEEVARMMDEIDADRDGFISLKEFAEFCKGDINSVGDGGVKELREAFDLYDQDSNGLIS 120

Query: 121 AAELHLVLSRLGEGCSVEDCSKMIQSVDADGDGNVSYEEFRKMMTKGK 168
           AAELH +L+RLGE CSV+DC+KMI SVD+DGDG VS+EEF+KMMT  K
Sbjct: 121 AAELHQILTRLGESCSVQDCTKMITSVDSDGDGYVSFEEFKKMMTTSK 164
[Close]
BLAST of Spo23654.1 vs. ExPASy Swiss-Prot
Match: ALL8_OLEEU (Calcium-binding allergen Ole e 8 OS=Olea europaea PE=1 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 4.600e-43
Identity = 90/158 (56.96%), Postives = 118/158 (74.68%), Query Frame = 1 

		  

Query: 16  HSLSSVYLSDTAEVKKVFDRFDVNGDGKISAEELIGVMKALGSETSPEEVAQMMEEMDTD 75
           +S  SVYL +  EV+ VF+RFD NGDGKIS +EL GV+KALGS TS EE+ ++MEE+DTD
Sbjct: 8   NSKPSVYLQEPNEVQGVFNRFDANGDGKISGDELAGVLKALGSNTSKEEIGRIMEEIDTD 67

Query: 76  RDGFINLEEFADFCSRKVE--GDESGSQELHDAFKMYDQDENGLISAAELHLVLSRLGEG 135
           +DGFIN++EFA F   + +      G  EL +AF++YDQD NGLIS+ ELH +L+RLGE 
Sbjct: 68  KDGFINVQEFAAFVKAETDPYPSSGGENELKEAFELYDQDHNGLISSVELHKILTRLGER 127

Query: 136 CSVEDCSKMIQSVDADGDGNVSYEEFRKMMTKGKSTSS 172
            +  DC +MI+SVD+DGDG VS+EEF+KMMT     +S
Sbjct: 128 YAEHDCVEMIKSVDSDGDGYVSFEEFKKMMTNKSGNNS 165
[Close]
BLAST of Spo23654.1 vs. ExPASy Swiss-Prot
Match: CML27_ARATH (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana GN=CML27 PE=1 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 5.600e-41
Identity = 86/154 (55.84%), Postives = 117/154 (75.97%), Query Frame = 1 

		  

Query: 19  SSVYLSDTAEVKKVFDRFDVNGDGKISAEELIGVMKALGSETSPEEVAQMMEEMDTDRDG 78
           ++V +++  E+KKVFD+FD NGDGKIS  EL GV KA+G+  +  E+ +++EE+DTDRDG
Sbjct: 14  ATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDG 73

Query: 79  FINLEEFADFCSRKVEGDESGSQELHDAFKMYDQDENGLISAAELHLVLSRLGEGCSVED 138
           +INL+EF+  C        S + E+ DAF +YDQD+NGLISA+ELH VL+RLG  CSVED
Sbjct: 74  YINLDEFSTLCR-----SSSSAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVED 133

Query: 139 CSKMIQSVDADGDGNVSYEEFRKMMTKGKSTSSS 173
           C++MI  VDADGDGNV++EEF+KMMT     +S+
Sbjct: 134 CTRMIGPVDADGDGNVNFEEFQKMMTSSSLLNSN 162
[Close]
BLAST of Spo23654.1 vs. ExPASy Swiss-Prot
Match: CML18_ORYSJ (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica GN=CML18 PE=2 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 1.700e-37
Identity = 85/151 (56.29%), Postives = 110/151 (72.85%), Query Frame = 1 

		  

Query: 27  AEVKKVFDRFDVNGDGKISAEELIGVMKALGSETSPEEVAQMMEEMDTDRDGFINLEEFA 86
           AE+++VF R+D NGDGKISAEEL  V++ALG+   P EV +MM+EMD+DRDGF++L EFA
Sbjct: 4   AELEQVFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEFA 63

Query: 87  DF-CSRK---------VEGDESGSQ-ELHDAFKMYDQDENGLISAAELHLVLSRLGEGCS 146
            F C             +  E+ S+ EL +AF+MYD D NG ISA ELH VL +LG+ CS
Sbjct: 64  AFHCGPTPAHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGDKCS 123

Query: 147 VEDCSKMIQSVDADGDGNVSYEEFRKMMTKG 167
           V DCS+MI+SVDADGDG V+++EF+KMM  G
Sbjct: 124 VADCSRMIRSVDADGDGCVNFDEFKKMMGGG 154
[Close]
BLAST of Spo23654.1 vs. ExPASy Swiss-Prot
Match: CML26_ARATH (Probable calcium-binding protein CML26 OS=Arabidopsis thaliana GN=CML26 PE=1 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 5.400e-36
Identity = 82/160 (51.25%), Postives = 113/160 (70.62%), Query Frame = 1 

		  

Query: 5   TTEENSNKSSSHSLSSVYLSDTAEVKKVFDRFDVNGDGKISAEELIGVMKALGSETSPEE 64
           T  E++NKS++ S          E+KKVFD+FD NGDGKIS  EL  V K++G+  + EE
Sbjct: 4   TNLESTNKSTTPSTDM-------ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEE 63

Query: 65  VAQMMEEMDTDRDGFINLEEFADFCSRKVEGDESGSQELHDAFKMYDQDENGLISAAELH 124
           + ++++E+D D DGFIN EEFA  C        S + E+ +AF +YDQ++NGLIS++E+H
Sbjct: 64  LNRVLDEIDIDCDGFINQEEFATICR-----SSSSAVEIREAFDLYDQNKNGLISSSEIH 123

Query: 125 LVLSRLGEGCSVEDCSKMIQSVDADGDGNVSYEEFRKMMT 165
            VL+RLG  CSVEDC +MI  VD DGDGNV++EEF+KMM+
Sbjct: 124 KVLNRLGMTCSVEDCVRMIGHVDTDGDGNVNFEEFQKMMS 151
[Close]
BLAST of Spo23654.1 vs. ExPASy Swiss-Prot
Match: CML24_ARATH (Calcium-binding protein CML24 OS=Arabidopsis thaliana GN=CML24 PE=2 SV=2)

HSP 1 Score: 141.7 bits (356), Expect = 7.300e-33
Identity = 76/157 (48.41%), Postives = 102/157 (64.97%), Query Frame = 1 

		  

Query: 13  SSSHSLSSVYLSDTAEVKKVFDRFDVNGDGKISAEELIGVMKALGSETSPEEVAQMMEEM 72
           SS + +    L    ++KKVF RFD NGDGKIS +EL  V++AL    SPEE   MM++ 
Sbjct: 2   SSKNGVVRSCLGSMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQF 61

Query: 73  DTDRDGFINLEEFADFCSRKVEG---DESGSQELHDAFKMYDQDENGLISAAELHLVLSR 132
           D D +GFI+L+EF       + G   + +   +L +AF++YD D NG ISA ELH V+  
Sbjct: 62  DLDGNGFIDLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKN 121

Query: 133 LGEGCSVEDCSKMIQSVDADGDGNVSYEEFRKMMTKG 167
           LGE CSV+DC KMI  VD DGDG V+++EF+KMM+ G
Sbjct: 122 LGEKCSVQDCKKMISKVDIDGDGCVNFDEFKKMMSNG 158
[Close]
BLAST of Spo23654.1 vs. TAIR (Arabidopsis)
Match: AT1G18210.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 168.7 bits (426), Expect = 3.200e-42
Identity = 86/154 (55.84%), Postives = 117/154 (75.97%), Query Frame = 1 

		  

Query: 19  SSVYLSDTAEVKKVFDRFDVNGDGKISAEELIGVMKALGSETSPEEVAQMMEEMDTDRDG 78
           ++V +++  E+KKVFD+FD NGDGKIS  EL GV KA+G+  +  E+ +++EE+DTDRDG
Sbjct: 14  ATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDG 73

Query: 79  FINLEEFADFCSRKVEGDESGSQELHDAFKMYDQDENGLISAAELHLVLSRLGEGCSVED 138
           +INL+EF+  C        S + E+ DAF +YDQD+NGLISA+ELH VL+RLG  CSVED
Sbjct: 74  YINLDEFSTLCR-----SSSSAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVED 133

Query: 139 CSKMIQSVDADGDGNVSYEEFRKMMTKGKSTSSS 173
           C++MI  VDADGDGNV++EEF+KMMT     +S+
Sbjct: 134 CTRMIGPVDADGDGNVNFEEFQKMMTSSSLLNSN 162
[Close]
BLAST of Spo23654.1 vs. TAIR (Arabidopsis)
Match: AT1G73630.1 (EF hand calcium-binding protein family)

HSP 1 Score: 152.1 bits (383), Expect = 3.100e-37
Identity = 82/160 (51.25%), Postives = 113/160 (70.62%), Query Frame = 1 

		  

Query: 5   TTEENSNKSSSHSLSSVYLSDTAEVKKVFDRFDVNGDGKISAEELIGVMKALGSETSPEE 64
           T  E++NKS++ S          E+KKVFD+FD NGDGKIS  EL  V K++G+  + EE
Sbjct: 4   TNLESTNKSTTPSTDM-------ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEE 63

Query: 65  VAQMMEEMDTDRDGFINLEEFADFCSRKVEGDESGSQELHDAFKMYDQDENGLISAAELH 124
           + ++++E+D D DGFIN EEFA  C        S + E+ +AF +YDQ++NGLIS++E+H
Sbjct: 64  LNRVLDEIDIDCDGFINQEEFATICR-----SSSSAVEIREAFDLYDQNKNGLISSSEIH 123

Query: 125 LVLSRLGEGCSVEDCSKMIQSVDADGDGNVSYEEFRKMMT 165
            VL+RLG  CSVEDC +MI  VD DGDGNV++EEF+KMM+
Sbjct: 124 KVLNRLGMTCSVEDCVRMIGHVDTDGDGNVNFEEFQKMMS 151
[Close]
BLAST of Spo23654.1 vs. TAIR (Arabidopsis)
Match: AT5G37770.1 (EF hand calcium-binding protein family)

HSP 1 Score: 141.7 bits (356), Expect = 4.100e-34
Identity = 76/157 (48.41%), Postives = 102/157 (64.97%), Query Frame = 1 

		  

Query: 13  SSSHSLSSVYLSDTAEVKKVFDRFDVNGDGKISAEELIGVMKALGSETSPEEVAQMMEEM 72
           SS + +    L    ++KKVF RFD NGDGKIS +EL  V++AL    SPEE   MM++ 
Sbjct: 2   SSKNGVVRSCLGSMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQF 61

Query: 73  DTDRDGFINLEEFADFCSRKVEG---DESGSQELHDAFKMYDQDENGLISAAELHLVLSR 132
           D D +GFI+L+EF       + G   + +   +L +AF++YD D NG ISA ELH V+  
Sbjct: 62  DLDGNGFIDLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKN 121

Query: 133 LGEGCSVEDCSKMIQSVDADGDGNVSYEEFRKMMTKG 167
           LGE CSV+DC KMI  VD DGDG V+++EF+KMM+ G
Sbjct: 122 LGEKCSVQDCKKMISKVDIDGDGCVNFDEFKKMMSNG 158
[Close]
BLAST of Spo23654.1 vs. TAIR (Arabidopsis)
Match: AT1G66400.1 (calmodulin like 23)

HSP 1 Score: 136.7 bits (343), Expect = 1.300e-32
Identity = 75/150 (50.00%), Postives = 97/150 (64.67%), Query Frame = 1 

		  

Query: 15  SHSLSSVYLSDTAEVKKVFDRFDVNGDGKISAEELIGVMKALGSETSPEEVAQMMEEMDT 74
           S ++S   L    ++KKVF RFD N DGKIS +EL  V+ AL    S EE   MM+E D 
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 61

Query: 75  DRDGFINLEEF-ADFCSRKVEGDESGSQELHDAFKMYDQDENGLISAAELHLVLSRLGEG 134
           D +GFI+L+EF A F       + S  ++L +AF +YD D NG ISA ELH V+  LGE 
Sbjct: 62  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 121

Query: 135 CSVEDCSKMIQSVDADGDGNVSYEEFRKMM 164
           CS++DC +MI  VD+DGDG V +EEF+KMM
Sbjct: 122 CSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151
[Close]
BLAST of Spo23654.1 vs. TAIR (Arabidopsis)
Match: AT1G24620.1 (EF hand calcium-binding protein family)

HSP 1 Score: 131.3 bits (329), Expect = 5.600e-31
Identity = 68/143 (47.55%), Postives = 97/143 (67.83%), Query Frame = 1 

		  

Query: 24  SDTAEVKKVFDRFDVNGDGKISAEELIGVMKALGSETSPEEVAQMMEEMDTDRDGFINLE 83
           ++  E++ VF +FDVNGDGKIS++EL  +M +LG E   EE+ + + E+D   DG+IN E
Sbjct: 33  TEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFE 92

Query: 84  EFADFCSRKVEGDESGSQELHDAFKMYDQDENGLISAAELHLVLSRLGEGCSVEDCSKMI 143
           EF +  ++ ++ ++   + L DAF +YD D NG ISA ELH VL  LG+ CS+ +C KMI
Sbjct: 93  EFVELNTKGMDQNDV-LENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMI 152

Query: 144 QSVDADGDGNVSYEEFRKMMTKG 167
             VD DGDG + +EEF+ MMT G
Sbjct: 153 GGVDKDGDGTIDFEEFKIMMTMG 174
The following BLAST results are available for this feature:
BLAST of Spo23654.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902185423|gb|KNA10488.1|1.4e-87100.hypothetical protein SOVF_1435... [more]
gi|731311858|ref|XP_010671432.1|9.8e-7082.2PREDICTED: calcium-binding all... [more]
gi|661898388|emb|CDO98355.1|7.8e-5162.7unnamed protein product [Coffe... [more]
gi|698502976|ref|XP_009797110.1|8.6e-5063.4PREDICTED: calcium-binding all... [more]
gi|697175630|ref|XP_009596756.1|1.5e-4963.8PREDICTED: calcium-binding all... [more]
back to top
BLAST of Spo23654.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9QUU9_SPIOL9.5e-88100.Uncharacterized protein OS=Spi... [more]
A0A0J8D893_BETVU6.8e-7082.2Uncharacterized protein OS=Bet... [more]
A0A068TPW5_COFCA5.4e-5162.7Uncharacterized protein OS=Cof... [more]
K4BM30_SOLLC4.0e-4660.7Uncharacterized protein OS=Sol... [more]
A0A0V0HFM4_SOLCH5.3e-4660.7Putative calcium-binding aller... [more]
back to top
BLAST of Spo23654.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5
Match NameE-valueIdentityDescription
ALL8_OLEEU4.6e-4356.9Calcium-binding allergen Ole e... [more]
CML27_ARATH5.6e-4155.8Probable calcium-binding prote... [more]
CML18_ORYSJ1.7e-3756.2Probable calcium-binding prote... [more]
CML26_ARATH5.4e-3651.2Probable calcium-binding prote... [more]
CML24_ARATH7.3e-3348.4Calcium-binding protein CML24 ... [more]
back to top
BLAST of Spo23654.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5
Match NameE-valueIdentityDescription
AT1G18210.13.2e-4255.8Calcium-binding EF-hand family... [more]
AT1G73630.13.1e-3751.2EF hand calcium-binding protei... [more]
AT5G37770.14.1e-3448.4EF hand calcium-binding protei... [more]
AT1G66400.11.3e-3250.0calmodulin like 23[more]
AT1G24620.15.6e-3147.5EF hand calcium-binding protei... [more]
back to top
InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 101..163
score: 8.9E-13coord: 29..89
score: 8.8
IPR002048EF-hand domainSMARTSM00054efh_1coord: 102..130
score: 4.8E-4coord: 64..92
score: 1.7E-6coord: 28..56
score: 1.9E-7coord: 138..166
score: 6.
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 134..169
score: 13.174coord: 98..133
score: 12.923coord: 24..59
score: 16.94coord: 60..95
score: 13
IPR011992EF-hand domain pairGENE3D1.10.238.10coord: 26..165
score: 2.1
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 25..166
score: 3.82
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 147..159
score: -coord: 111..123
score: -coord: 73..85
score: -coord: 37..49
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 28..166
score: 5.4
NoneNo IPR availablePANTHERPTHR10891:SF605CALCIUM-BINDING PROTEIN CML25-RELATEDcoord: 28..166
score: 5.4

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010466 negative regulation of peptidase activity
cellular_component GO:0005618 cell wall
cellular_component GO:0005576 extracellular region
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0004869 cysteine-type endopeptidase inhibitor activity
RNA-Seq Expression
   



Co-expression
Gener valueExpression
Spo201700.68Barchart | Table
Spo237800.67Barchart | Table