Spo24307 (gene)

Overview
NameSpo24307
Typegene
OrganismSpinacia oleracea (Spinach)
DescriptionMitotic-spindle organizing protein 1 (Mitotic-spindle organizing protein associated with a ring of gamma-tubulin 1)
LocationSpoScf_00067 : 33658 .. 33867 (+)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCAGAAGCTGCACGCACTGCGCGAGACTCTTTAGAATTGGCATTTCAAATGTCAAACATACTTGATACAGGAGTAGATCGCCACACTCTCTCTGTCCTAATCGCATTATGTGATCTTGGCTTAAACCCTGAAGCGCTTGCCGCTGTTGTCAAGGAGCTTAGAACAGACCCCTCACTTCCTTCAACCATTCCACCTGATTCCTGA

mRNA sequence

ATGGATTCAGAAGCTGCACGCACTGCGCGAGACTCTTTAGAATTGGCATTTCAAATGTCAAACATACTTGATACAGGAGTAGATCGCCACACTCTCTCTGTCCTAATCGCATTATGTGATCTTGGCTTAAACCCTGAAGCGCTTGCCGCTGTTGTCAAGGAGCTTAGAACAGACCCCTCACTTCCTTCAACCATTCCACCTGATTCCTGA

Coding sequence (CDS)

ATGGATTCAGAAGCTGCACGCACTGCGCGAGACTCTTTAGAATTGGCATTTCAAATGTCAAACATACTTGATACAGGAGTAGATCGCCACACTCTCTCTGTCCTAATCGCATTATGTGATCTTGGCTTAAACCCTGAAGCGCTTGCCGCTGTTGTCAAGGAGCTTAGAACAGACCCCTCACTTCCTTCAACCATTCCACCTGATTCCTGA

Protein sequence

MDSEAARTARDSLELAFQMSNILDTGVDRHTLSVLIALCDLGLNPEALAAVVKELRTDPSLPSTIPPDS
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo24307.1Spo24307.1mRNA


Homology
[Close]
BLAST of Spo24307.1 vs. NCBI nr
Match: gi|902048919|gb|KNA02850.1| (hypothetical protein SOVF_214750 [Spinacia oleracea])

HSP 1 Score: 133.7 bits (335), Expect = 1.300e-28
Identity = 69/69 (100.00%), Postives = 69/69 (100.00%), Query Frame = 1 

		  

Query: 1  MDSEAARTARDSLELAFQMSNILDTGVDRHTLSVLIALCDLGLNPEALAAVVKELRTDPS 60
          MDSEAARTARDSLELAFQMSNILDTGVDRHTLSVLIALCDLGLNPEALAAVVKELRTDPS
Sbjct: 1  MDSEAARTARDSLELAFQMSNILDTGVDRHTLSVLIALCDLGLNPEALAAVVKELRTDPS 60

Query: 61 LPSTIPPDS 70
          LPSTIPPDS
Sbjct: 61 LPSTIPPDS 69
[Close]
BLAST of Spo24307.1 vs. NCBI nr
Match: gi|731324600|ref|XP_010673057.1| (PREDICTED: mitotic-spindle organizing protein 1B-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 118.6 bits (296), Expect = 4.200e-24
Identity = 62/69 (89.86%), Postives = 65/69 (94.20%), Query Frame = 1 

		  

Query: 1  MDSEAARTARDSLELAFQMSNILDTGVDRHTLSVLIALCDLGLNPEALAAVVKELRTDPS 60
          MDSEAARTARDSLELAFQMSNILDTG+DRHTLSVLIALCDLGLNPEALAAVVKELR +PS
Sbjct: 1  MDSEAARTARDSLELAFQMSNILDTGLDRHTLSVLIALCDLGLNPEALAAVVKELRREPS 60

Query: 61 LPSTIPPDS 70
            STIP D+
Sbjct: 61 PSSTIPADA 69
[Close]
BLAST of Spo24307.1 vs. NCBI nr
Match: gi|1000941381|ref|XP_015582673.1| (PREDICTED: mitotic-spindle organizing protein 1A [Ricinus communis])

HSP 1 Score: 105.1 bits (261), Expect = 4.800e-20
Identity = 54/66 (81.82%), Postives = 59/66 (89.39%), Query Frame = 1 

		  

Query: 1  MDSEAARTARDSLELAFQMSNILDTGVDRHTLSVLIALCDLGLNPEALAAVVKELRTDPS 60
          MD EAA+TAR+SL+LAF MSNILDTG+DRHTLSVLIALCDLGLNPEALAAVVKELR  PS
Sbjct: 1  MDPEAAKTARESLDLAFHMSNILDTGLDRHTLSVLIALCDLGLNPEALAAVVKELRQQPS 60

Query: 61 LPSTIP 67
             +IP
Sbjct: 61 SSPSIP 66
[Close]
BLAST of Spo24307.1 vs. NCBI nr
Match: gi|920689027|gb|KOM33010.1| (hypothetical protein LR48_Vigan01g256600 [Vigna angularis])

HSP 1 Score: 104.8 bits (260), Expect = 6.300e-20
Identity = 55/67 (82.09%), Postives = 62/67 (92.54%), Query Frame = 1 

		  

Query: 1  MDSEAARTARDSLELAFQMSNILDTGVDRHTLSVLIALCDLGLNPEALAAVVKELRTD-P 60
          MDSEAAR AR+SL+LAF MSNILDTG+DRHTLSVLIALCDLG+NPEALAAVVKELR + P
Sbjct: 1  MDSEAARAARESLDLAFHMSNILDTGLDRHTLSVLIALCDLGVNPEALAAVVKELRKEKP 60

Query: 61 SLPSTIP 67
          SL S++P
Sbjct: 61 SLSSSLP 67
[Close]
BLAST of Spo24307.1 vs. NCBI nr
Match: gi|356504256|ref|XP_003520913.1| (PREDICTED: mitotic-spindle organizing protein 1B [Glycine max])

HSP 1 Score: 104.8 bits (260), Expect = 6.300e-20
Identity = 55/67 (82.09%), Postives = 62/67 (92.54%), Query Frame = 1 

		  

Query: 1  MDSEAARTARDSLELAFQMSNILDTGVDRHTLSVLIALCDLGLNPEALAAVVKELRTD-P 60
          MD EAARTAR+SL+LAF MSNILDTG+DRHTLSVLIALCDLG+NPEALAAVVKELR + P
Sbjct: 1  MDPEAARTARESLDLAFHMSNILDTGLDRHTLSVLIALCDLGVNPEALAAVVKELRKEKP 60

Query: 61 SLPSTIP 67
          SL S++P
Sbjct: 61 SLSSSLP 67
[Close]
BLAST of Spo24307.1 vs. UniProtKB/TrEMBL
Match: A0A0K9Q6X9_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_214750 PE=4 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 8.900e-29
Identity = 69/69 (100.00%), Postives = 69/69 (100.00%), Query Frame = 1 

		  

Query: 1  MDSEAARTARDSLELAFQMSNILDTGVDRHTLSVLIALCDLGLNPEALAAVVKELRTDPS 60
          MDSEAARTARDSLELAFQMSNILDTGVDRHTLSVLIALCDLGLNPEALAAVVKELRTDPS
Sbjct: 1  MDSEAARTARDSLELAFQMSNILDTGVDRHTLSVLIALCDLGLNPEALAAVVKELRTDPS 60

Query: 61 LPSTIPPDS 70
          LPSTIPPDS
Sbjct: 61 LPSTIPPDS 69
[Close]
BLAST of Spo24307.1 vs. UniProtKB/TrEMBL
Match: A0A0J8FH33_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g063220 PE=4 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 2.900e-24
Identity = 62/69 (89.86%), Postives = 65/69 (94.20%), Query Frame = 1 

		  

Query: 1  MDSEAARTARDSLELAFQMSNILDTGVDRHTLSVLIALCDLGLNPEALAAVVKELRTDPS 60
          MDSEAARTARDSLELAFQMSNILDTG+DRHTLSVLIALCDLGLNPEALAAVVKELR +PS
Sbjct: 1  MDSEAARTARDSLELAFQMSNILDTGLDRHTLSVLIALCDLGLNPEALAAVVKELRREPS 60

Query: 61 LPSTIPPDS 70
            STIP D+
Sbjct: 61 PSSTIPADA 69
[Close]
BLAST of Spo24307.1 vs. UniProtKB/TrEMBL
Match: B9T1I8_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0186670 PE=4 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 3.400e-20
Identity = 54/66 (81.82%), Postives = 59/66 (89.39%), Query Frame = 1 

		  

Query: 1  MDSEAARTARDSLELAFQMSNILDTGVDRHTLSVLIALCDLGLNPEALAAVVKELRTDPS 60
          MD EAA+TAR+SL+LAF MSNILDTG+DRHTLSVLIALCDLGLNPEALAAVVKELR  PS
Sbjct: 1  MDPEAAKTARESLDLAFHMSNILDTGLDRHTLSVLIALCDLGLNPEALAAVVKELRQQPS 60

Query: 61 LPSTIP 67
             +IP
Sbjct: 61 SSPSIP 66
[Close]
BLAST of Spo24307.1 vs. UniProtKB/TrEMBL
Match: A0A0B2PDD0_GLYSO (Mitotic-spindle organizing protein 1B OS=Glycine soja GN=glysoja_038443 PE=4 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 4.400e-20
Identity = 55/67 (82.09%), Postives = 62/67 (92.54%), Query Frame = 1 

		  

Query: 1  MDSEAARTARDSLELAFQMSNILDTGVDRHTLSVLIALCDLGLNPEALAAVVKELRTD-P 60
          MD EAARTAR+SL+LAF MSNILDTG+DRHTLSVLIALCDLG+NPEALAAVVKELR + P
Sbjct: 1  MDPEAARTARESLDLAFHMSNILDTGLDRHTLSVLIALCDLGVNPEALAAVVKELRKEKP 60

Query: 61 SLPSTIP 67
          SL S++P
Sbjct: 61 SLSSSLP 67
[Close]
BLAST of Spo24307.1 vs. UniProtKB/TrEMBL
Match: C6T5S8_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_03G068900 PE=2 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 4.400e-20
Identity = 55/67 (82.09%), Postives = 62/67 (92.54%), Query Frame = 1 

		  

Query: 1  MDSEAARTARDSLELAFQMSNILDTGVDRHTLSVLIALCDLGLNPEALAAVVKELRTD-P 60
          MD EAARTAR+SL+LAF MSNILDTG+DRHTLSVLIALCDLG+NPEALAAVVKELR + P
Sbjct: 1  MDPEAARTARESLDLAFHMSNILDTGLDRHTLSVLIALCDLGVNPEALAAVVKELRKEKP 60

Query: 61 SLPSTIP 67
          SL S++P
Sbjct: 61 SLSSSLP 67
[Close]
BLAST of Spo24307.1 vs. ExPASy Swiss-Prot
Match: MZT1B_ARATH (Mitotic-spindle organizing protein 1B OS=Arabidopsis thaliana GN=GIP1 PE=1 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 2.200e-20
Identity = 50/65 (76.92%), Postives = 59/65 (90.77%), Query Frame = 1 

		  

Query: 1  MDSEAARTARDSLELAFQMSNILDTGVDRHTLSVLIALCDLGLNPEALAAVVKELRTDPS 60
          MD EA+RTAR+SLEL F+MSNILDTG+DRHTLSVLIALCDLG+NPEALA VVKELR + S
Sbjct: 1  MDEEASRTARESLELVFRMSNILDTGLDRHTLSVLIALCDLGVNPEALATVVKELRRE-S 60

Query: 61 LPSTI 66
          +P ++
Sbjct: 61 IPDSV 64
[Close]
BLAST of Spo24307.1 vs. ExPASy Swiss-Prot
Match: MZT1A_ARATH (Mitotic-spindle organizing protein 1A OS=Arabidopsis thaliana GN=GIP2 PE=1 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 4.100e-19
Identity = 47/64 (73.44%), Postives = 56/64 (87.50%), Query Frame = 1 

		  

Query: 1  MDSEAARTARDSLELAFQMSNILDTGVDRHTLSVLIALCDLGLNPEALAAVVKELRTDPS 60
          M+ EAA TAR+SLEL F+MSNIL+TG+DRHTLSVLIALCD+GLNPEALA +VKELR D +
Sbjct: 1  MNQEAAETARESLELVFRMSNILETGLDRHTLSVLIALCDIGLNPEALATLVKELRRDSA 60

Query: 61 LPST 65
            +T
Sbjct: 61 TTTT 64
[Close]
BLAST of Spo24307.1 vs. ExPASy Swiss-Prot
Match: MZT1_PICSI (Mitotic-spindle organizing protein 1 OS=Picea sitchensis PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 7.200e-16
Identity = 41/58 (70.69%), Postives = 50/58 (86.21%), Query Frame = 1 

		  

Query: 1  MDSEAARTARDSLELAFQMSNILDTGVDRHTLSVLIALCDLGLNPEALAAVVKELRTD 59
          MD  AA+ AR+SL+LAF +SN L TG+DRHTLS+LIALC+ G+NPEALAAVVKELR +
Sbjct: 1  MDRTAAQNARESLDLAFSISNFLQTGLDRHTLSILIALCEHGVNPEALAAVVKELRRE 58
[Close]
BLAST of Spo24307.1 vs. ExPASy Swiss-Prot
Match: MZT1_TAEGU (Mitotic-spindle organizing protein 1 OS=Taeniopygia guttata GN=mzt1 PE=3 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.500e-8
Identity = 28/58 (48.28%), Postives = 44/58 (75.86%), Query Frame = 1 

		  

Query: 1  MDSEAA--RTARDSLELAFQMSNILDTGVDRHTLSVLIALCDLGLNPEALAAVVKELR 57
          M S AA     R+++++ F++S IL+TG+D  TLS+ + LC+ G+NPEAL++V+KELR
Sbjct: 1  MASNAASLNAVRETMDVLFEISRILNTGLDMETLSICVRLCEQGINPEALSSVIKELR 58
[Close]
BLAST of Spo24307.1 vs. ExPASy Swiss-Prot
Match: MZT1_MOUSE (Mitotic-spindle organizing protein 1 OS=Mus musculus GN=Mzt1 PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 9.500e-8
Identity = 23/47 (48.94%), Postives = 39/47 (82.98%), Query Frame = 1 

		  

Query: 10 RDSLELAFQMSNILDTGVDRHTLSVLIALCDLGLNPEALAAVVKELR 57
          R+++++  ++S IL+TG+D  TLS+ + LC+ G+NPEAL++V+KELR
Sbjct: 19 RETMDVLLEISRILNTGLDMETLSICVRLCEQGINPEALSSVIKELR 65
[Close]
BLAST of Spo24307.1 vs. TAIR (Arabidopsis)
Match: AT4G09550.1 (AtGCP3 interacting protein 1)

HSP 1 Score: 99.0 bits (245), Expect = 1.200e-21
Identity = 50/65 (76.92%), Postives = 59/65 (90.77%), Query Frame = 1 

		  

Query: 1  MDSEAARTARDSLELAFQMSNILDTGVDRHTLSVLIALCDLGLNPEALAAVVKELRTDPS 60
          MD EA+RTAR+SLEL F+MSNILDTG+DRHTLSVLIALCDLG+NPEALA VVKELR + S
Sbjct: 1  MDEEASRTARESLELVFRMSNILDTGLDRHTLSVLIALCDLGVNPEALATVVKELRRE-S 60

Query: 61 LPSTI 66
          +P ++
Sbjct: 61 IPDSV 64
[Close]
BLAST of Spo24307.1 vs. TAIR (Arabidopsis)
Match: AT1G73790.1 (Protein of unknown function (DUF3743))

HSP 1 Score: 94.7 bits (234), Expect = 2.300e-20
Identity = 47/64 (73.44%), Postives = 56/64 (87.50%), Query Frame = 1 

		  

Query: 1  MDSEAARTARDSLELAFQMSNILDTGVDRHTLSVLIALCDLGLNPEALAAVVKELRTDPS 60
          M+ EAA TAR+SLEL F+MSNIL+TG+DRHTLSVLIALCD+GLNPEALA +VKELR D +
Sbjct: 1  MNQEAAETARESLELVFRMSNILETGLDRHTLSVLIALCDIGLNPEALATLVKELRRDSA 60

Query: 61 LPST 65
            +T
Sbjct: 61 TTTT 64
The following BLAST results are available for this feature:
BLAST of Spo24307.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902048919|gb|KNA02850.1|1.3e-28100.hypothetical protein SOVF_2147... [more]
gi|731324600|ref|XP_010673057.1|4.2e-2489.8PREDICTED: mitotic-spindle org... [more]
gi|1000941381|ref|XP_015582673.1|4.8e-2081.8PREDICTED: mitotic-spindle org... [more]
gi|920689027|gb|KOM33010.1|6.3e-2082.0hypothetical protein LR48_Viga... [more]
gi|356504256|ref|XP_003520913.1|6.3e-2082.0PREDICTED: mitotic-spindle org... [more]
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BLAST of Spo24307.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9Q6X9_SPIOL8.9e-29100.Uncharacterized protein OS=Spi... [more]
A0A0J8FH33_BETVU2.9e-2489.8Uncharacterized protein OS=Bet... [more]
B9T1I8_RICCO3.4e-2081.8Putative uncharacterized prote... [more]
A0A0B2PDD0_GLYSO4.4e-2082.0Mitotic-spindle organizing pro... [more]
C6T5S8_SOYBN4.4e-2082.0Uncharacterized protein OS=Gly... [more]
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BLAST of Spo24307.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5
Match NameE-valueIdentityDescription
MZT1B_ARATH2.2e-2076.9Mitotic-spindle organizing pro... [more]
MZT1A_ARATH4.1e-1973.4Mitotic-spindle organizing pro... [more]
MZT1_PICSI7.2e-1670.6Mitotic-spindle organizing pro... [more]
MZT1_TAEGU1.5e-848.2Mitotic-spindle organizing pro... [more]
MZT1_MOUSE9.5e-848.9Mitotic-spindle organizing pro... [more]
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BLAST of Spo24307.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 2
Match NameE-valueIdentityDescription
AT4G09550.11.2e-2176.9AtGCP3 interacting protein 1[more]
AT1G73790.12.3e-2073.4Protein of unknown function (D... [more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR022214Mitotic-spindle organizing protein 1PANTHERPTHR28520FAMILY NOT NAMEDcoord: 1..58
score: 4.8
IPR022214Mitotic-spindle organizing protein 1PFAMPF12554MOZART1coord: 11..56
score: 1.8

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0010155 regulation of proton transport
biological_process GO:0035304 regulation of protein dephosphorylation
biological_process GO:0046777 protein autophosphorylation
biological_process GO:0042793 plastid transcription
biological_process GO:0016556 mRNA modification
biological_process GO:0044237 cellular metabolic process
biological_process GO:0016226 iron-sulfur cluster assembly
biological_process GO:0006289 nucleotide-excision repair
biological_process GO:0019761 glucosinolate biosynthetic process
biological_process GO:0009902 chloroplast relocation
biological_process GO:0009073 aromatic amino acid family biosynthetic process
biological_process GO:0010207 photosystem II assembly
biological_process GO:0033566 gamma-tubulin complex localization
biological_process GO:0006285 base-excision repair, AP site formation
biological_process GO:0000737 DNA catabolic process, endonucleolytic
biological_process GO:0006364 rRNA processing
biological_process GO:0006368 transcription elongation from RNA polymerase II promoter
biological_process GO:0015031 protein transport
biological_process GO:0045893 positive regulation of transcription, DNA-templated
biological_process GO:0009733 response to auxin
biological_process GO:0009407 toxin catabolic process
biological_process GO:0009636 response to toxic substance
biological_process GO:0010583 response to cyclopentenone
biological_process GO:0010731 protein glutathionylation
biological_process GO:0008152 metabolic process
biological_process GO:0019852 L-ascorbic acid metabolic process
biological_process GO:0050794 regulation of cellular process
biological_process GO:0006351 transcription, DNA-templated
biological_process GO:0006749 glutathione metabolic process
biological_process GO:0006412 translation
biological_process GO:0010027 thylakoid membrane organization
biological_process GO:0016578 histone deubiquitination
biological_process GO:0006406 mRNA export from nucleus
biological_process GO:0006306 DNA methylation
cellular_component GO:0005634 nucleus
cellular_component GO:0009570 chloroplast stroma
cellular_component GO:0008274 gamma-tubulin ring complex
cellular_component GO:0009941 chloroplast envelope
cellular_component GO:0005667 transcription factor complex
cellular_component GO:0071819 DUBm complex
cellular_component GO:0005643 nuclear pore
cellular_component GO:0000124 SAGA complex
cellular_component GO:0070390 transcription export complex 2
cellular_component GO:0009534 chloroplast thylakoid
cellular_component GO:0009507 chloroplast
cellular_component GO:0005886 plasma membrane
cellular_component GO:0009840 chloroplastic endopeptidase Clp complex
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0022857 transmembrane transporter activity
molecular_function GO:0045174 glutathione dehydrogenase (ascorbate) activity
molecular_function GO:0004364 glutathione transferase activity
molecular_function GO:0005515 protein binding
molecular_function GO:0005524 ATP binding
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0016874 ligase activity
molecular_function GO:0016757 transferase activity, transferring glycosyl groups
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003723 RNA binding
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0003684 damaged DNA binding
molecular_function GO:0003713 transcription coactivator activity
molecular_function GO:0016655 oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0046914 transition metal ion binding
molecular_function GO:0008534 oxidized purine nucleobase lesion DNA N-glycosylase activity
molecular_function GO:0046983 protein dimerization activity
RNA-Seq Expression