Spo24774 (gene)

Overview
NameSpo24774
Typegene
OrganismSpinacia oleracea (Spinach)
DescriptionMediator of RNA polymerase II transcription subunit 13, putative
Locationchr5 : 5443520 .. 5443966 (+)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAACAAAACAAGAGAGAACAAATAGTTGAACAAAAACAACATGGAAGAATAATGGAATATGAACTCATAAAAGCCGTTGCTCAAGCTTGGCATAGTCACTTCAACAATGAAAAGGAAGCCACGAGCGAATTCGATGCTCGAAGGCGTAACTTTCGTAACAAGCCAACGAGGTTTAAACTCGAGGTCGAGGCTAAGAACAAGTTGAAGGTTGATGAAGAAGCGAGTAAAGGGATATGGAACTTTAACCAGTCTCTTTGGGATCCCTTTGAGATCGTTGCGGTGTCTAAAAGGTTGGAAACCGGGTTAGTTTTGAACTATGATTTTGATGATTTGCAAAAATTGAATGAGCAATTGAGGGGGTTTAAGAAAAGGAAGGAGAGTAAGAATAGTCTTAGGAACTTGTTTAATATCATGTCTTCTAGGAGATTTAGTGATGATAGATAA

mRNA sequence

ATGAAACAAAACAAGAGAGAACAAATAGTTGAACAAAAACAACATGGAAGAATAATGGAATATGAACTCATAAAAGCCGTTGCTCAAGCTTGGCATAGTCACTTCAACAATGAAAAGGAAGCCACGAGCGAATTCGATGCTCGAAGGCGTAACTTTCGTAACAAGCCAACGAGGTTTAAACTCGAGGTCGAGGCTAAGAACAAGTTGAAGGTTGATGAAGAAGCGAGTAAAGGGATATGGAACTTTAACCAGTCTCTTTGGGATCCCTTTGAGATCGTTGCGGTGTCTAAAAGGTTGGAAACCGGGTTAGTTTTGAACTATGATTTTGATGATTTGCAAAAATTGAATGAGCAATTGAGGGGGTTTAAGAAAAGGAAGGAGAGTAAGAATAGTCTTAGGAACTTGTTTAATATCATGTCTTCTAGGAGATTTAGTGATGATAGATAA

Coding sequence (CDS)

ATGAAACAAAACAAGAGAGAACAAATAGTTGAACAAAAACAACATGGAAGAATAATGGAATATGAACTCATAAAAGCCGTTGCTCAAGCTTGGCATAGTCACTTCAACAATGAAAAGGAAGCCACGAGCGAATTCGATGCTCGAAGGCGTAACTTTCGTAACAAGCCAACGAGGTTTAAACTCGAGGTCGAGGCTAAGAACAAGTTGAAGGTTGATGAAGAAGCGAGTAAAGGGATATGGAACTTTAACCAGTCTCTTTGGGATCCCTTTGAGATCGTTGCGGTGTCTAAAAGGTTGGAAACCGGGTTAGTTTTGAACTATGATTTTGATGATTTGCAAAAATTGAATGAGCAATTGAGGGGGTTTAAGAAAAGGAAGGAGAGTAAGAATAGTCTTAGGAACTTGTTTAATATCATGTCTTCTAGGAGATTTAGTGATGATAGATAA

Protein sequence

MKQNKREQIVEQKQHGRIMEYELIKAVAQAWHSHFNNEKEATSEFDARRRNFRNKPTRFKLEVEAKNKLKVDEEASKGIWNFNQSLWDPFEIVAVSKRLETGLVLNYDFDDLQKLNEQLRGFKKRKESKNSLRNLFNIMSSRRFSDDR
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo24774.1Spo24774.1mRNA


Homology
[Close]
BLAST of Spo24774.1 vs. NCBI nr
Match: gi|731335741|ref|XP_010678875.1| (PREDICTED: uncharacterized protein LOC104894355 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 215.3 bits (547), Expect = 7.100e-53
Identity = 110/154 (71.43%), Postives = 128/154 (83.12%), Query Frame = 1 

		  

Query: 1   MKQNKRE-------QIVEQKQHGRIMEYELIKAVAQAWHSHFNNEKEATSEFDARRRNFR 60
           MKQNKRE       Q  + +QH +  + E++KAVAQAWHSHFNN +EAT EFD RRRNFR
Sbjct: 1   MKQNKREVHLEHQQQQQQHQQHDQQKKVEVLKAVAQAWHSHFNNAREATCEFDVRRRNFR 60

Query: 61  NKPTRFKLEVEAKNKLKVDEEASKGIWNFNQSLWDPFEIVAVSKRLETGLVLNYDFDDLQ 120
           NKP+RFKLE+EA NKLK+DEEA   +WNFNQSLWDP+EIVAVSKRLETGLVLNYDFDD+ 
Sbjct: 61  NKPSRFKLELEATNKLKIDEEAKNTLWNFNQSLWDPYEIVAVSKRLETGLVLNYDFDDMN 120

Query: 121 KLNEQLRGFKKRKESKNSLRNLFNIMSSRRFSDD 148
            ++  +R FKKRKESKNSLRNLFNIMSSRRF+D+
Sbjct: 121 NIDGHMRVFKKRKESKNSLRNLFNIMSSRRFNDN 154
[Close]
BLAST of Spo24774.1 vs. NCBI nr
Match: gi|697156507|ref|XP_009587008.1| (PREDICTED: uncharacterized protein LOC104084761 [Nicotiana tomentosiformis])

HSP 1 Score: 122.5 bits (306), Expect = 6.300e-25
Identity = 72/147 (48.98%), Postives = 104/147 (70.75%), Query Frame = 1 

		  

Query: 1   MKQNKRE-QIVEQKQHGRIMEYELIKAVAQAWHSHFNNEKEATSEFDARRRNFRNKPTRF 60
           MK NK++ +  + +QH    + E++KAVAQAWH H ++ +  T+EFDA R NF+NKP+RF
Sbjct: 1   MKNNKQQLEPYQDEQH----DIEILKAVAQAWHGH-SSSRRTTAEFDAHRLNFKNKPSRF 60

Query: 61  KLEVEAKNKLKVDEEASKGIWNFNQSLWDPFEIVAVSKRLETGLVLNYDFDDLQKLNEQL 120
           KLE  +K   + +     G W+F+QSLWD +EIV +SK+LE GL L++ F     L+E +
Sbjct: 61  KLEAMSKATAR-EYYGGTGSWDFSQSLWDSYEIVNLSKKLEAGLALDHSFSG---LDETI 120

Query: 121 RGFKKRKESKNSLRNLFNIMSSRRFSD 147
           R  +KRKESKNSLR+LFN +SSRR+++
Sbjct: 121 RIGQKRKESKNSLRSLFNRVSSRRYNE 138
[Close]
BLAST of Spo24774.1 vs. NCBI nr
Match: gi|922371161|ref|XP_013457742.1| (hypothetical protein MTR_4g104870 [Medicago truncatula])

HSP 1 Score: 122.1 bits (305), Expect = 8.200e-25
Identity = 72/139 (51.80%), Postives = 96/139 (69.06%), Query Frame = 1 

		  

Query: 10  VEQKQH--GRIMEYELIKAVAQAWHSHFNNEKEATSEFDARRRNFRNKPTRFKLEVEAKN 69
           +++KQH      + E++KAVAQAW++H  + K   SEFDARRRNFR KP+RFKLE   K+
Sbjct: 1   MKKKQHHENHNQDLEVLKAVAQAWYNHSGSVK-PLSEFDARRRNFRGKPSRFKLETLTKS 60

Query: 70  KLKVDEEASKGIWNFNQSLWDPFEIVAVSKRLETGLVLNYDFDDL-QKLNEQLRGFKKRK 129
               +  +S    +F  SLWDP+E+V VS+R+ETGL L+  FDD     + QL+G   ++
Sbjct: 61  SSSRETNSSTYYCDFQHSLWDPYELVTVSRRIETGLALDNPFDDFCVSKSVQLKG---KR 120

Query: 130 ESKNSLRNLFNIMSSRRFS 146
           ESKNSLRNLFN MSSRRF+
Sbjct: 121 ESKNSLRNLFNNMSSRRFN 135
[Close]
BLAST of Spo24774.1 vs. NCBI nr
Match: gi|645268004|ref|XP_008239335.1| (PREDICTED: uncharacterized protein LOC103337939 [Prunus mume])

HSP 1 Score: 121.7 bits (304), Expect = 1.100e-24
Identity = 70/134 (52.24%), Postives = 95/134 (70.90%), Query Frame = 1 

		  

Query: 14  QHGRIMEYELIKAVAQAWHSHFNNEKEATSEFDARRRNFRNK-PTRFKLEVEAKNKLKVD 73
           Q G   E E++KAVAQAW+SH +  +  TSEFDA RRNFR + P+RFKLE   K+    +
Sbjct: 6   QPGDDRELEILKAVAQAWYSHSSGSR-PTSEFDAHRRNFRGRLPSRFKLEAMRKSTSASN 65

Query: 74  EEASKGIWNFNQSLWDPFEIVAVSKRLETGLVLNYDFDDLQKLNEQLRGFKKRKESKNSL 133
           + +    W+F QSLWD +EIVAVSKRLETGL +  D +   +L+ Q R  ++R+ESK+SL
Sbjct: 66  QASGYAAWDFGQSLWDAYEIVAVSKRLETGLFV--DENPFNQLDSQTRVIRRRRESKHSL 125

Query: 134 RNLFNIMSSRRFSD 147
           R+LF+ MSSRRF++
Sbjct: 126 RSLFDKMSSRRFNE 136
[Close]
BLAST of Spo24774.1 vs. NCBI nr
Match: gi|1009175864|ref|XP_015869119.1| (PREDICTED: uncharacterized protein LOC107406504 [Ziziphus jujuba])

HSP 1 Score: 121.3 bits (303), Expect = 1.400e-24
Identity = 68/127 (53.54%), Postives = 88/127 (69.29%), Query Frame = 1 

		  

Query: 20  EYELIKAVAQAWHSHFNNEKEATSEFDARRRNFRNKPTRFKLEVEAKNKLKVDEEASKGI 79
           + E++KAVAQAWHSH  + +   +EFDA RRNF  KP+RFK  +EA  K   +   S G 
Sbjct: 16  QVEILKAVAQAWHSHSGSSRPM-NEFDAHRRNFNGKPSRFK--IEAMGKRAAERNGSCGT 75

Query: 80  WNFNQSLWDPFEIVAVSKRLETGLVLNYDFDDLQKLNEQLRGFKKRKESKNSLRNLFNIM 139
           W+F QSLWD +EIV +SKRLE GLV  +D     + +  +R  +KRKESKNSLR+LFN M
Sbjct: 76  WDFKQSLWDSYEIVTMSKRLEAGLV--FDNIPFTEADAHVRVHRKRKESKNSLRSLFNQM 135

Query: 140 SSRRFSD 147
           SSRRF++
Sbjct: 136 SSRRFNE 137
[Close]
BLAST of Spo24774.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CAR8_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_5g118160 PE=4 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 5.000e-53
Identity = 110/154 (71.43%), Postives = 128/154 (83.12%), Query Frame = 1 

		  

Query: 1   MKQNKRE-------QIVEQKQHGRIMEYELIKAVAQAWHSHFNNEKEATSEFDARRRNFR 60
           MKQNKRE       Q  + +QH +  + E++KAVAQAWHSHFNN +EAT EFD RRRNFR
Sbjct: 1   MKQNKREVHLEHQQQQQQHQQHDQQKKVEVLKAVAQAWHSHFNNAREATCEFDVRRRNFR 60

Query: 61  NKPTRFKLEVEAKNKLKVDEEASKGIWNFNQSLWDPFEIVAVSKRLETGLVLNYDFDDLQ 120
           NKP+RFKLE+EA NKLK+DEEA   +WNFNQSLWDP+EIVAVSKRLETGLVLNYDFDD+ 
Sbjct: 61  NKPSRFKLELEATNKLKIDEEAKNTLWNFNQSLWDPYEIVAVSKRLETGLVLNYDFDDMN 120

Query: 121 KLNEQLRGFKKRKESKNSLRNLFNIMSSRRFSDD 148
            ++  +R FKKRKESKNSLRNLFNIMSSRRF+D+
Sbjct: 121 NIDGHMRVFKKRKESKNSLRNLFNIMSSRRFNDN 154
[Close]
BLAST of Spo24774.1 vs. UniProtKB/TrEMBL
Match: A0A072URT5_MEDTR (Uncharacterized protein OS=Medicago truncatula GN=MTR_4g104870 PE=4 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 5.700e-25
Identity = 72/139 (51.80%), Postives = 96/139 (69.06%), Query Frame = 1 

		  

Query: 10  VEQKQH--GRIMEYELIKAVAQAWHSHFNNEKEATSEFDARRRNFRNKPTRFKLEVEAKN 69
           +++KQH      + E++KAVAQAW++H  + K   SEFDARRRNFR KP+RFKLE   K+
Sbjct: 1   MKKKQHHENHNQDLEVLKAVAQAWYNHSGSVK-PLSEFDARRRNFRGKPSRFKLETLTKS 60

Query: 70  KLKVDEEASKGIWNFNQSLWDPFEIVAVSKRLETGLVLNYDFDDL-QKLNEQLRGFKKRK 129
               +  +S    +F  SLWDP+E+V VS+R+ETGL L+  FDD     + QL+G   ++
Sbjct: 61  SSSRETNSSTYYCDFQHSLWDPYELVTVSRRIETGLALDNPFDDFCVSKSVQLKG---KR 120

Query: 130 ESKNSLRNLFNIMSSRRFS 146
           ESKNSLRNLFN MSSRRF+
Sbjct: 121 ESKNSLRNLFNNMSSRRFN 135
[Close]
BLAST of Spo24774.1 vs. UniProtKB/TrEMBL
Match: A0A068UWG0_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00037338001 PE=4 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 3.700e-24
Identity = 70/147 (47.62%), Postives = 97/147 (65.99%), Query Frame = 1 

		  

Query: 5   KREQIVEQKQHGRIMEY--ELIKAVAQAWHSHFNNEKEATSEFDARRRNFRNKPTRFKLE 64
           K++Q  EQ+ H    E+  E+IKAVAQAW+++ ++ K + +EFDA RRNF+ KP+RFKLE
Sbjct: 3   KKQQQQEQRVHDGENEHDLEIIKAVAQAWYANSSSSKSSPNEFDAHRRNFKGKPSRFKLE 62

Query: 65  VEAKNKL---KVDEEASKGIWNFNQSLWDPFEIVAVSKRLETGLVLNYDFDDLQKLNEQL 124
              K           ++   W+F QSLWD +EIV +++RLE GLVL++ F +        
Sbjct: 63  AARKASSGDGYGSSSSTSSNWDFGQSLWDSYEIVTIARRLERGLVLDHTFSEKDDAGHV- 122

Query: 125 RGFKKRKESKNSLRNLFNIMSSRRFSD 147
             FKKRKESKNSLRNL N +SSRRF++
Sbjct: 123 --FKKRKESKNSLRNLLNRISSRRFNE 146
[Close]
BLAST of Spo24774.1 vs. UniProtKB/TrEMBL
Match: A0A103XWI5_CYNCS (Uncharacterized protein OS=Cynara cardunculus var. scolymus GN=Ccrd_023601 PE=4 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 3.700e-24
Identity = 72/135 (53.33%), Postives = 87/135 (64.44%), Query Frame = 1 

		  

Query: 12  QKQHGRIMEYELIKAVAQAWHSHFNN----EKEATSEFDARRRNFRNKPTRFKLEVEAKN 71
           Q+QH    E E+IKAVAQAWH H       +  AT+EFDARR NF+NKPTRFKLE   K 
Sbjct: 9   QEQH----ELEIIKAVAQAWHGHSTATAAPDPTATNEFDARRLNFKNKPTRFKLEAIMKP 68

Query: 72  KLKVDEEASKGIWNFNQSLWDPFEIVAVSKRLETGLVLNYDFDDLQKLNEQLRGFKKRKE 131
               D       W+F QSLWD +EIVAVS+RLE GL+L  DFD+    + Q +  K++KE
Sbjct: 69  SRNKDGSERATSWDFRQSLWDSYEIVAVSRRLENGLLLGDDFDE----STQSQVGKRKKE 128

Query: 132 SKNSLRNLFNIMSSR 143
           SKNSLRN+ N  S R
Sbjct: 129 SKNSLRNVLNRTSKR 135
[Close]
BLAST of Spo24774.1 vs. UniProtKB/TrEMBL
Match: M5WLX8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa024800mg PE=4 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 1.100e-23
Identity = 67/128 (52.34%), Postives = 91/128 (71.09%), Query Frame = 1 

		  

Query: 20  EYELIKAVAQAWHSHFNNEKEATSEFDARRRNFRNK-PTRFKLEVEAKNKLKVDEEASKG 79
           E E++KAVAQAW+SH +  +  TSEFDA RRNF  + P+RFKLE   K+    ++ +   
Sbjct: 12  ELEILKAVAQAWYSHSSGSR-PTSEFDAHRRNFTGRLPSRFKLEAMRKSTSASNQASGYA 71

Query: 80  IWNFNQSLWDPFEIVAVSKRLETGLVLNYDFDDLQKLNEQLRGFKKRKESKNSLRNLFNI 139
            W+F QSLWD +EIVAVSKRLETGL +  D +   KL+   R  ++R+ESK+SLR+LF+ 
Sbjct: 72  AWDFGQSLWDAYEIVAVSKRLETGLFV--DENPFNKLDSPTRVIRRRRESKHSLRSLFDK 131

Query: 140 MSSRRFSD 147
           MSSRRF++
Sbjct: 132 MSSRRFNE 136
[Close]
BLAST of Spo24774.1 vs. TAIR (Arabidopsis)
Match: AT4G24700.1 (unknown protein)

HSP 1 Score: 84.7 bits (208), Expect = 5.100e-17
Identity = 59/151 (39.07%), Postives = 85/151 (56.29%), Query Frame = 1 

		  

Query: 2   KQNKREQIVEQKQHGRIMEYELIKAVAQAWHSHFNNEKEATSEFDARRRN--FRNKPTRF 61
           K+ + +   E+++  +    EL+KA AQAW SH    K    EFDA+R++   + K +RF
Sbjct: 3   KKREAKDENEEEEEEKKKRLELMKAAAQAWLSHSQTSKHTVLEFDAQRKHAFVKGKASRF 62

Query: 62  KLEVEAKNKLKVDEEASKGIWNFNQSLWDPFEIVAVSKRLETGLVL------NYDFDDLQ 121
           K E      L      S   W F QSLWDP+EI++VSK+LE  L L      + D D L+
Sbjct: 63  KTEA-----LSAKHHPSFLDWEFGQSLWDPYEILSVSKKLERELTLEEQTFSSSDNDGLK 122

Query: 122 KLNEQLRGFKKRKESKNSLRNLFNIMSSRRF 145
           K+ +     KK ++S+NSLR+LF   SS+RF
Sbjct: 123 KMKK-----KKTRDSRNSLRSLFTRSSSKRF 143
The following BLAST results are available for this feature:
BLAST of Spo24774.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|731335741|ref|XP_010678875.1|7.1e-5371.4PREDICTED: uncharacterized pro... [more]
gi|697156507|ref|XP_009587008.1|6.3e-2548.9PREDICTED: uncharacterized pro... [more]
gi|922371161|ref|XP_013457742.1|8.2e-2551.8hypothetical protein MTR_4g104... [more]
gi|645268004|ref|XP_008239335.1|1.1e-2452.2PREDICTED: uncharacterized pro... [more]
gi|1009175864|ref|XP_015869119.1|1.4e-2453.5PREDICTED: uncharacterized pro... [more]
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BLAST of Spo24774.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0J8CAR8_BETVU5.0e-5371.4Uncharacterized protein OS=Bet... [more]
A0A072URT5_MEDTR5.7e-2551.8Uncharacterized protein OS=Med... [more]
A0A068UWG0_COFCA3.7e-2447.6Uncharacterized protein OS=Cof... [more]
A0A103XWI5_CYNCS3.7e-2453.3Uncharacterized protein OS=Cyn... [more]
M5WLX8_PRUPE1.1e-2352.3Uncharacterized protein OS=Pru... [more]
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BLAST of Spo24774.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Spo24774.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1
Match NameE-valueIdentityDescription
AT4G24700.15.1e-1739.0unknown protein[more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableunknownCoilCoilcoord: 112..132
scor
NoneNo IPR availablePANTHERPTHR34665FAMILY NOT NAMEDcoord: 22..146
score: 4.3

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030163 protein catabolic process
biological_process GO:0044262 cellular carbohydrate metabolic process
biological_process GO:0006351 transcription, DNA-templated
biological_process GO:0006355 regulation of transcription, DNA-templated
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0006396 RNA processing
biological_process GO:0006508 proteolysis
biological_process GO:0006090 pyruvate metabolic process
biological_process GO:0006108 malate metabolic process
biological_process GO:0046487 glyoxylate metabolic process
biological_process GO:0019643 reductive tricarboxylic acid cycle
biological_process GO:0006810 transport
biological_process GO:0008152 metabolic process
cellular_component GO:0009536 plastid
cellular_component GO:0016020 membrane
cellular_component GO:0005667 transcription factor complex
cellular_component GO:0005622 intracellular
cellular_component GO:0005840 ribosome
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016758 transferase activity, transferring hexosyl groups
molecular_function GO:0016757 transferase activity, transferring glycosyl groups
molecular_function GO:0043565 sequence-specific DNA binding
molecular_function GO:0046982 protein heterodimerization activity
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0020037 heme binding
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0005506 iron ion binding
molecular_function GO:0008233 peptidase activity
molecular_function GO:0030060 L-malate dehydrogenase activity
molecular_function GO:0005515 protein binding
molecular_function GO:0016740 transferase activity
molecular_function GO:0005215 transporter activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0003677 DNA binding
RNA-Seq Expression