Spo24931 (gene) | SpinachBase

Overview


Name	Spo24931
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Cold-regulated protein, putative
Location	chr1 : 29915032 .. 29915262 (-)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCAATGGTGGGGGTGCCAATATGTGTTGAGTGTGGGACCAGGAGCAACCCTTGTAGGTGCAAGATAGTGGGTCCCACAGTCGGGTTCTTAGCGTTTGTGGTGGCGGCGGTGGTGGAGTGGCCGGTGGGGATGTTGGTGTACCCGTTTAAGCACAGGAAAGGCCGTAAGATCATGGGTCATCCTGTTTCTGTAGTTTATCCTAAAGTTAACAATGCTATTCCTATTTGA

mRNA sequence

ATGCCAATGGTGGGGGTGCCAATATGTGTTGAGTGTGGGACCAGGAGCAACCCTTGTAGGTGCAAGATAGTGGGTCCCACAGTCGGGTTCTTAGCGTTTGTGGTGGCGGCGGTGGTGGAGTGGCCGGTGGGGATGTTGGTGTACCCGTTTAAGCACAGGAAAGGCCGTAAGATCATGGGTCATCCTGTTTCTGTAGTTTATCCTAAAGTTAACAATGCTATTCCTATTTGA

Coding sequence (CDS)

ATGCCAATGGTGGGGGTGCCAATATGTGTTGAGTGTGGGACCAGGAGCAACCCTTGTAGGTGCAAGATAGTGGGTCCCACAGTCGGGTTCTTAGCGTTTGTGGTGGCGGCGGTGGTGGAGTGGCCGGTGGGGATGTTGGTGTACCCGTTTAAGCACAGGAAAGGCCGTAAGATCATGGGTCATCCTGTTTCTGTAGTTTATCCTAAAGTTAACAATGCTATTCCTATTTGA

Protein sequence

MPMVGVPICVECGTRSNPCRCKIVGPTVGFLAFVVAAVVEWPVGMLVYPFKHRKGRKIMGHPVSVVYPKVNNAIPI

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo24931.1	Spo24931.1	mRNA

Homology

[Close]

BLAST of Spo24931.1 vs. NCBI nr
Match: gi|902239343|gb|KNA25569.1| (hypothetical protein SOVF_005220 [Spinacia oleracea])

HSP 1 Score: 162.9 bits (411), Expect = 2.200e-37
Identity = 76/76 (100.00%), Postives = 76/76 (100.00%), Query Frame = 1


		  
Query: 1  MPMVGVPICVECGTRSNPCRCKIVGPTVGFLAFVVAAVVEWPVGMLVYPFKHRKGRKIMG 60
          MPMVGVPICVECGTRSNPCRCKIVGPTVGFLAFVVAAVVEWPVGMLVYPFKHRKGRKIMG
Sbjct: 1  MPMVGVPICVECGTRSNPCRCKIVGPTVGFLAFVVAAVVEWPVGMLVYPFKHRKGRKIMG 60

Query: 61 HPVSVVYPKVNNAIPI 77
          HPVSVVYPKVNNAIPI
Sbjct: 61 HPVSVVYPKVNNAIPI 76

[Close]

BLAST of Spo24931.1 vs. NCBI nr
Match: gi|731316004|ref|XP_010693833.1| (PREDICTED: uncharacterized protein LOC104906731 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 149.8 bits (377), Expect = 1.900e-33
Identity = 68/76 (89.47%), Postives = 72/76 (94.74%), Query Frame = 1


		  
Query: 1  MPMVGVPICVECGTRSNPCRCKIVGPTVGFLAFVVAAVVEWPVGMLVYPFKHRKGRKIMG 60
          MP++GVPICVECGTRSNPCRCKIVGPTVGFLAF VAAVVEWPVG+LVYPFKH KGRKIMG
Sbjct: 1  MPILGVPICVECGTRSNPCRCKIVGPTVGFLAFAVAAVVEWPVGLLVYPFKHMKGRKIMG 60

Query: 61 HPVSVVYPKVNNAIPI 77
          HP SVVYP V+NAIPI
Sbjct: 61 HPASVVYPSVSNAIPI 76

[Close]

BLAST of Spo24931.1 vs. NCBI nr
Match: gi|922380840|ref|XP_013460649.1| (cold-regulated protein, putative [Medicago truncatula])

HSP 1 Score: 137.5 bits (345), Expect = 9.700e-30
Identity = 61/73 (83.56%), Postives = 68/73 (93.15%), Query Frame = 1


		  
Query: 4  VGVPICVECGTRSNPCRCKIVGPTVGFLAFVVAAVVEWPVGMLVYPFKHRKGRKIMGHPV 63
          VGVPICV+CGTRSNPCRCK+VGPT+GF+AFV AAVVEWPVG LVY FKH+KGR+IMGHP 
Sbjct: 4  VGVPICVQCGTRSNPCRCKVVGPTLGFVAFVAAAVVEWPVGALVYIFKHKKGRRIMGHPA 63

Query: 64 SVVYPKVNNAIPI 77
          +VVYPKVN AIPI
Sbjct: 64 TVVYPKVNRAIPI 76

[Close]

BLAST of Spo24931.1 vs. NCBI nr
Match: gi|922380842|ref|XP_013460650.1| (cold-regulated protein, putative [Medicago truncatula])

HSP 1 Score: 135.2 bits (339), Expect = 4.800e-29
Identity = 59/72 (81.94%), Postives = 66/72 (91.67%), Query Frame = 1


		  
Query: 5  GVPICVECGTRSNPCRCKIVGPTVGFLAFVVAAVVEWPVGMLVYPFKHRKGRKIMGHPVS 64
          GVP+CV+CGTRSNPCRCK+VGPT+GF+AFVVA VVEWPVG LVY FKHRKGR+IMGHP +
Sbjct: 5  GVPLCVQCGTRSNPCRCKVVGPTIGFVAFVVAGVVEWPVGALVYIFKHRKGRRIMGHPAT 64

Query: 65 VVYPKVNNAIPI 77
          VVYPKV  AIPI
Sbjct: 65 VVYPKVTRAIPI 76

[Close]

BLAST of Spo24931.1 vs. NCBI nr
Match: gi|950951494|ref|XP_014495693.1| (PREDICTED: uncharacterized protein LOC106757537 [Vigna radiata var. radiata])

HSP 1 Score: 134.4 bits (337), Expect = 8.200e-29
Identity = 59/73 (80.82%), Postives = 65/73 (89.04%), Query Frame = 1


		  
Query: 4  VGVPICVECGTRSNPCRCKIVGPTVGFLAFVVAAVVEWPVGMLVYPFKHRKGRKIMGHPV 63
          +GVPICVECGTRSNPCRCK+VGPT+GF+AF  AAVVEWPVG LVY F+H KGRKIMGHP 
Sbjct: 4  IGVPICVECGTRSNPCRCKVVGPTLGFVAFAAAAVVEWPVGALVYCFRHAKGRKIMGHPA 63

Query: 64 SVVYPKVNNAIPI 77
          SV+YP V NAIPI
Sbjct: 64 SVIYPSVTNAIPI 76

[Close]

BLAST of Spo24931.1 vs. UniProtKB/TrEMBL
Match: A0A0K9S2Z0_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_005220 PE=4 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 1.500e-37
Identity = 76/76 (100.00%), Postives = 76/76 (100.00%), Query Frame = 1


		  
Query: 1  MPMVGVPICVECGTRSNPCRCKIVGPTVGFLAFVVAAVVEWPVGMLVYPFKHRKGRKIMG 60
          MPMVGVPICVECGTRSNPCRCKIVGPTVGFLAFVVAAVVEWPVGMLVYPFKHRKGRKIMG
Sbjct: 1  MPMVGVPICVECGTRSNPCRCKIVGPTVGFLAFVVAAVVEWPVGMLVYPFKHRKGRKIMG 60

Query: 61 HPVSVVYPKVNNAIPI 77
          HPVSVVYPKVNNAIPI
Sbjct: 61 HPVSVVYPKVNNAIPI 76

[Close]

BLAST of Spo24931.1 vs. UniProtKB/TrEMBL
Match: A0A072UZW1_MEDTR (Cold-regulated protein, putative OS=Medicago truncatula GN=MTR_3g067715 PE=4 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 6.800e-30
Identity = 61/73 (83.56%), Postives = 68/73 (93.15%), Query Frame = 1


		  
Query: 4  VGVPICVECGTRSNPCRCKIVGPTVGFLAFVVAAVVEWPVGMLVYPFKHRKGRKIMGHPV 63
          VGVPICV+CGTRSNPCRCK+VGPT+GF+AFV AAVVEWPVG LVY FKH+KGR+IMGHP 
Sbjct: 4  VGVPICVQCGTRSNPCRCKVVGPTLGFVAFVAAAVVEWPVGALVYIFKHKKGRRIMGHPA 63

Query: 64 SVVYPKVNNAIPI 77
          +VVYPKVN AIPI
Sbjct: 64 TVVYPKVNRAIPI 76

[Close]

BLAST of Spo24931.1 vs. UniProtKB/TrEMBL
Match: A0A072UXZ4_MEDTR (Cold-regulated protein, putative OS=Medicago truncatula GN=MTR_3g067720 PE=4 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 3.400e-29
Identity = 59/72 (81.94%), Postives = 66/72 (91.67%), Query Frame = 1


		  
Query: 5  GVPICVECGTRSNPCRCKIVGPTVGFLAFVVAAVVEWPVGMLVYPFKHRKGRKIMGHPVS 64
          GVP+CV+CGTRSNPCRCK+VGPT+GF+AFVVA VVEWPVG LVY FKHRKGR+IMGHP +
Sbjct: 5  GVPLCVQCGTRSNPCRCKVVGPTIGFVAFVVAGVVEWPVGALVYIFKHRKGRRIMGHPAT 64

Query: 65 VVYPKVNNAIPI 77
          VVYPKV  AIPI
Sbjct: 65 VVYPKVTRAIPI 76

[Close]

BLAST of Spo24931.1 vs. UniProtKB/TrEMBL
Match: V7D2E4_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_001G231900g PE=4 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 1.300e-28
Identity = 58/73 (79.45%), Postives = 65/73 (89.04%), Query Frame = 1


		  
Query: 4   VGVPICVECGTRSNPCRCKIVGPTVGFLAFVVAAVVEWPVGMLVYPFKHRKGRKIMGHPV 63
           +GVPICV+CGTRSNPCRCK+VGPT+GF+AF  AAVVEWPVG LVY F+H KGRKIMGHP 
Sbjct: 62  IGVPICVQCGTRSNPCRCKVVGPTLGFVAFAAAAVVEWPVGALVYCFRHTKGRKIMGHPA 121

Query: 64  SVVYPKVNNAIPI 77
           SV+YP V NAIPI
Sbjct: 122 SVIYPSVTNAIPI 134

[Close]

BLAST of Spo24931.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BWG0_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_8g183490 PE=4 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 1.300e-28
Identity = 58/74 (78.38%), Postives = 66/74 (89.19%), Query Frame = 1


		  
Query: 3  MVGVPICVECGTRSNPCRCKIVGPTVGFLAFVVAAVVEWPVGMLVYPFKHRKGRKIMGHP 62
          M   PICVECGTR NPCRCK+VGPT+GFLAFVV A+VEWPVGM+VY F H KGRKIMGHP
Sbjct: 1  MTKAPICVECGTRDNPCRCKVVGPTLGFLAFVVTAIVEWPVGMVVYIFNHSKGRKIMGHP 60

Query: 63 VSVVYPKVNNAIPI 77
          ++VVYPK++NAIPI
Sbjct: 61 LTVVYPKISNAIPI 74

[Close]

BLAST of Spo24931.1 vs. TAIR (Arabidopsis)
Match: AT3G03341.1 (unknown protein)

HSP 1 Score: 118.2 bits (295), Expect = 2.100e-27
Identity = 50/71 (70.42%), Postives = 60/71 (84.51%), Query Frame = 1


		  
Query: 6  VPICVECGTRSNPCRCKIVGPTVGFLAFVVAAVVEWPVGMLVYPFKHRKGRKIMGHPVSV 65
          +PICV+CGT  NPCRCK+VGPT+G +AFV A +VEWPVG LVY FKH KGR+IMGHP + 
Sbjct: 2  IPICVQCGTGGNPCRCKVVGPTLGLVAFVAAGIVEWPVGALVYIFKHAKGRRIMGHPATH 61

Query: 66 VYPKVNNAIPI 77
          VYPKV+ +IPI
Sbjct: 62 VYPKVSRSIPI 72

The following BLAST results are available for this feature:

BLAST of Spo24931.1 vs. NCBI nr
BLAST of Spo24931.1 vs. UniProtKB/TrEMBL
BLAST of Spo24931.1 vs. ExPASy Swiss-Prot
BLAST of Spo24931.1 vs. TAIR (Arabidopsis)

BLAST of Spo24931.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902239343\|gb\|KNA25569.1\|	2.2e-37	100.	hypothetical protein SOVF_0052...	[more]
gi\|731316004\|ref\|XP_010693833.1\|	1.9e-33	89.4	PREDICTED: uncharacterized pro...	[more]
gi\|922380840\|ref\|XP_013460649.1\|	9.7e-30	83.5	cold-regulated protein, putati...	[more]
gi\|922380842\|ref\|XP_013460650.1\|	4.8e-29	81.9	cold-regulated protein, putati...	[more]
gi\|950951494\|ref\|XP_014495693.1\|	8.2e-29	80.8	PREDICTED: uncharacterized pro...	[more]

BLAST of Spo24931.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9S2Z0_SPIOL	1.5e-37	100.	Uncharacterized protein OS=Spi...	[more]
A0A072UZW1_MEDTR	6.8e-30	83.5	Cold-regulated protein, putati...	[more]
A0A072UXZ4_MEDTR	3.4e-29	81.9	Cold-regulated protein, putati...	[more]
V7D2E4_PHAVU	1.3e-28	79.4	Uncharacterized protein OS=Pha...	[more]
A0A0J8BWG0_BETVU	1.3e-28	78.3	Uncharacterized protein OS=Bet...	[more]

BLAST of Spo24931.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Spo24931.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1


Match Name	E-value	Identity	Description
AT3G03341.1	2.1e-27	70.4	unknown protein	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR34673	FAMILY NOT NAMED	coord: 1..76 score: 3.9

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009649	entrainment of circadian clock
biological_process	GO:0032259	methylation
biological_process	GO:0006857	oligopeptide transport
biological_process	GO:0006771	riboflavin metabolic process
biological_process	GO:0045892	negative regulation of transcription, DNA-templated
biological_process	GO:0006012	galactose metabolic process
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
biological_process	GO:0019497	hexachlorocyclohexane metabolic process
biological_process	GO:0016311	dephosphorylation
biological_process	GO:0000160	phosphorelay signal transduction system
biological_process	GO:0051762	sesquiterpene biosynthetic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0010167	response to nitrate
biological_process	GO:0042752	regulation of circadian rhythm
biological_process	GO:0006355	regulation of transcription, DNA-templated
biological_process	GO:0009408	response to heat
biological_process	GO:0043693	monoterpene biosynthetic process
biological_process	GO:0033383	geranyl diphosphate metabolic process
biological_process	GO:0042542	response to hydrogen peroxide
biological_process	GO:0048573	photoperiodism, flowering
biological_process	GO:0009644	response to high light intensity
biological_process	GO:0006508	proteolysis
biological_process	GO:0006950	response to stress
biological_process	GO:0006457	protein folding
biological_process	GO:0002238	response to molecule of fungal origin
biological_process	GO:0030001	metal ion transport
biological_process	GO:0015706	nitrate transport
biological_process	GO:0042753	positive regulation of circadian rhythm
biological_process	GO:0009585	red, far-red light phototransduction
biological_process	GO:0009909	regulation of flower development
biological_process	GO:0010114	response to red light
biological_process	GO:0006096	glycolytic process
biological_process	GO:0006396	RNA processing
biological_process	GO:0070475	rRNA base methylation
biological_process	GO:0030488	tRNA methylation
biological_process	GO:0006094	gluconeogenesis
biological_process	GO:0042273	ribosomal large subunit biogenesis
biological_process	GO:0006446	regulation of translational initiation
biological_process	GO:0009845	seed germination
biological_process	GO:0000381	regulation of alternative mRNA splicing, via spliceosome
biological_process	GO:0006448	regulation of translational elongation
biological_process	GO:0042256	mature ribosome assembly
biological_process	GO:0009846	pollen germination
biological_process	GO:0009793	embryo development ending in seed dormancy
biological_process	GO:0071215	cellular response to abscisic acid stimulus
biological_process	GO:0010106	cellular response to iron ion starvation
biological_process	GO:0006826	iron ion transport
biological_process	GO:0008152	metabolic process
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005730	nucleolus
cellular_component	GO:0009506	plasmodesma
cellular_component	GO:0017177	glucosidase II complex
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005634	nucleus
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0000932	P-body
cellular_component	GO:0005840	ribosome
cellular_component	GO:0005622	intracellular
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0031965	nuclear membrane
cellular_component	GO:0016020	membrane
cellular_component	GO:0009570	chloroplast stroma
cellular_component	GO:0005667	transcription factor complex
cellular_component	GO:0005773	vacuole
molecular_function	GO:0003743	translation initiation factor activity
molecular_function	GO:0010334	sesquiterpene synthase activity
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0008236	serine-type peptidase activity
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0043022	ribosome binding
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0042803	protein homodimerization activity
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0008173	RNA methyltransferase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0005515	protein binding
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0003924	GTPase activity
molecular_function	GO:0005525	GTP binding
molecular_function	GO:0003746	translation elongation factor activity
molecular_function	GO:0005215	transporter activity
molecular_function	GO:0043023	ribosomal large subunit binding
molecular_function	GO:0004558	alpha-1,4-glucosidase activity
molecular_function	GO:0030246	carbohydrate binding
molecular_function	GO:0032450	maltose alpha-glucosidase activity
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0000166	nucleotide binding
molecular_function	GO:0008234	cysteine-type peptidase activity
molecular_function	GO:0003993	acid phosphatase activity
molecular_function	GO:0004034	aldose 1-epimerase activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo11195	0.77	Barchart \| Table
Spo08124	0.76	Barchart \| Table
Spo06002	0.75	Barchart \| Table
Spo10269	0.74	Barchart \| Table
Spo09573	0.73	Barchart \| Table
Spo19748	0.72	Barchart \| Table
Spo25748	0.71	Barchart \| Table
Spo02239	0.71	Barchart \| Table
Spo03527	0.71	Barchart \| Table
Spo22023	0.71	Barchart \| Table
Spo12064	0.70	Barchart \| Table
Spo24573	0.70	Barchart \| Table
Spo25361	0.69	Barchart \| Table
Spo15731	0.69	Barchart \| Table
Spo23690	0.69	Barchart \| Table
Spo04137	0.69	Barchart \| Table
Spo13621	0.69	Barchart \| Table
Spo16460	0.68	Barchart \| Table
Spo20326	0.68	Barchart \| Table
Spo10165	0.68	Barchart \| Table
Spo16367	0.68	Barchart \| Table
Spo16857	0.68	Barchart \| Table
Spo00341	0.67	Barchart \| Table
Spo01314	0.67	Barchart \| Table
Spo01806	0.67	Barchart \| Table
Spo03739	0.67	Barchart \| Table
Spo15007	0.67	Barchart \| Table
Spo19724	0.67	Barchart \| Table
Spo26735	0.66	Barchart \| Table
Spo20053	0.66	Barchart \| Table
Spo21280	0.66	Barchart \| Table
Spo24503	0.66	Barchart \| Table
Spo11780	0.66	Barchart \| Table
Spo24785	0.66	Barchart \| Table
Spo04430	0.66	Barchart \| Table
Spo01110	0.66	Barchart \| Table
Spo18040	0.65	Barchart \| Table