Spo24940 (gene) | SpinachBase

Overview


Name	Spo24940
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Calcium binding protein
Location	chr1 : 30150216 .. 30150668 (+)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAAAAACTCAAACTCTCAGAAAAAATCAACAACCGAAATCGAGAGCATATTCCAGAAATTCGACGCAAACGGCGATGGAAAGATCTCAGCGACAGAACTGCAATCTCTGTTACAAGCCCTAGGATCGGAGACGTCGTCGGAAGAGGTGAAGAAGATGATGGACGAGATGGACAAAGATGGCGACGGTTACGTCGATCTAAGGGAACTCGAGGAGTTTCACGCCGGATCTGGCGGCAATGCGGGGTCCACTAAGGAGCTTCGAGAAGCATTCAGCTTGTATGATAAGGATGGCAATGGTAAGATTTCAGCGCGTGAGTTGTACGATGTGTTGAAGAGTCTTGGAGAGAAGTGTTCGTTGAAGGATTGTAAGAAGATGATTCAATCTGTTGATGCGGATGGTGATGGTCATGTTGATTTCGAGGAGTTTAAGAAGATGATGGCCGCTTGA

mRNA sequence

ATGGCGAAAAACTCAAACTCTCAGAAAAAATCAACAACCGAAATCGAGAGCATATTCCAGAAATTCGACGCAAACGGCGATGGAAAGATCTCAGCGACAGAACTGCAATCTCTGTTACAAGCCCTAGGATCGGAGACGTCGTCGGAAGAGGTGAAGAAGATGATGGACGAGATGGACAAAGATGGCGACGGTTACGTCGATCTAAGGGAACTCGAGGAGTTTCACGCCGGATCTGGCGGCAATGCGGGGTCCACTAAGGAGCTTCGAGAAGCATTCAGCTTGTATGATAAGGATGGCAATGGTAAGATTTCAGCGCGTGAGTTGTACGATGTGTTGAAGAGTCTTGGAGAGAAGTGTTCGTTGAAGGATTGTAAGAAGATGATTCAATCTGTTGATGCGGATGGTGATGGTCATGTTGATTTCGAGGAGTTTAAGAAGATGATGGCCGCTTGA

Coding sequence (CDS)

ATGGCGAAAAACTCAAACTCTCAGAAAAAATCAACAACCGAAATCGAGAGCATATTCCAGAAATTCGACGCAAACGGCGATGGAAAGATCTCAGCGACAGAACTGCAATCTCTGTTACAAGCCCTAGGATCGGAGACGTCGTCGGAAGAGGTGAAGAAGATGATGGACGAGATGGACAAAGATGGCGACGGTTACGTCGATCTAAGGGAACTCGAGGAGTTTCACGCCGGATCTGGCGGCAATGCGGGGTCCACTAAGGAGCTTCGAGAAGCATTCAGCTTGTATGATAAGGATGGCAATGGTAAGATTTCAGCGCGTGAGTTGTACGATGTGTTGAAGAGTCTTGGAGAGAAGTGTTCGTTGAAGGATTGTAAGAAGATGATTCAATCTGTTGATGCGGATGGTGATGGTCATGTTGATTTCGAGGAGTTTAAGAAGATGATGGCCGCTTGA

Protein sequence

MAKNSNSQKKSTTEIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDKDGDGYVDLRELEEFHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCSLKDCKKMIQSVDADGDGHVDFEEFKKMMAA

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo24940.1	Spo24940.1	mRNA

Homology

[Close]

BLAST of Spo24940.1 vs. NCBI nr
Match: gi|902208551|gb|KNA15979.1| (hypothetical protein SOVF_093330 [Spinacia oleracea])

HSP 1 Score: 287.0 bits (733), Expect = 2.000e-74
Identity = 149/150 (99.33%), Postives = 149/150 (99.33%), Query Frame = 1

		  
Query: 1   MAKNSNSQKKSTTEIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDK 60
           MAKNSNSQKKSTTEIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDK
Sbjct: 1   MAKNSNSQKKSTTEIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDK 60

Query: 61  DGDGYVDLRELEEFHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCS 120
           DGDGYVDLRE EEFHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCS
Sbjct: 61  DGDGYVDLREFEEFHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCS 120

Query: 121 LKDCKKMIQSVDADGDGHVDFEEFKKMMAA 151
           LKDCKKMIQSVDADGDGHVDFEEFKKMMAA
Sbjct: 121 LKDCKKMIQSVDADGDGHVDFEEFKKMMAA 150

[Close]

BLAST of Spo24940.1 vs. NCBI nr
Match: gi|731316034|ref|XP_010693960.1| (PREDICTED: probable calcium-binding protein CML18 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 246.1 bits (627), Expect = 3.800e-62
Identity = 130/150 (86.67%), Postives = 134/150 (89.33%), Query Frame = 1

		  
Query: 1   MAKNSNSQKKSTTEIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDK 60
           MAKNS        EIE IF KFDANGDGKISATEL+SLLQALGSETS+EEVKKMM+EMDK
Sbjct: 1   MAKNS--------EIERIFSKFDANGDGKISATELKSLLQALGSETSTEEVKKMMEEMDK 60

Query: 61  DGDGYVDLRELEEFHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCS 120
           DGDG+VDL E  EFHAGSGGNAGSTKEL EAF LYDKDGNGKISARELYDVLKSLGEKCS
Sbjct: 61  DGDGFVDLLEFSEFHAGSGGNAGSTKELEEAFRLYDKDGNGKISARELYDVLKSLGEKCS 120

Query: 121 LKDCKKMIQSVDADGDGHVDFEEFKKMMAA 151
           LKDCKKMI SVDADGDGHVDFEEFKKMMAA
Sbjct: 121 LKDCKKMISSVDADGDGHVDFEEFKKMMAA 142

[Close]

BLAST of Spo24940.1 vs. NCBI nr
Match: gi|356501039|ref|XP_003519336.1| (PREDICTED: probable calcium-binding protein CML18 [Glycine max])

HSP 1 Score: 180.6 bits (457), Expect = 2.000e-42
Identity = 85/135 (62.96%), Postives = 111/135 (82.22%), Query Frame = 1

		  
Query: 14  EIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDKDGDGYVDLRELEE 73
           E+  IF KFD NGDGKIS TEL+ +L ALGS+T+ EE+K+MM+E+D++GDG++DL+E  +
Sbjct: 4   EVRQIFNKFDKNGDGKISVTELKDMLAALGSKTTDEELKRMMEELDQNGDGFIDLKEFAD 63

Query: 74  FHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCSLKDCKKMIQSVDA 133
           FH   G     +KELR+AF LYD D NG ISA+EL+DVL++LGEKCSL DC++MI +VDA
Sbjct: 64  FHCNGGAGKDDSKELRDAFDLYDVDKNGLISAKELHDVLRNLGEKCSLSDCRRMISNVDA 123

Query: 134 DGDGHVDFEEFKKMM 149
           DGDG+V+FEEFKKMM
Sbjct: 124 DGDGNVNFEEFKKMM 138

[Close]

BLAST of Spo24940.1 vs. NCBI nr
Match: gi|950987490|ref|XP_014503786.1| (PREDICTED: probable calcium-binding protein CML23 [Vigna radiata var. radiata])

HSP 1 Score: 179.1 bits (453), Expect = 5.700e-42
Identity = 88/136 (64.71%), Postives = 110/136 (80.88%), Query Frame = 1

		  
Query: 14  EIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDKDGDGYVDLRELEE 73
           E+  IF KFD NGDGKIS TEL+ +L  LGS+T+ EE+K+MM+E+DK+GDGY+DL+E  +
Sbjct: 4   EVRQIFNKFDKNGDGKISITELRDMLGTLGSKTTKEELKRMMEELDKNGDGYIDLKEFAD 63

Query: 74  FHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCSLKDCKKMIQSVDA 133
           FH    GN  S KELR+AF LYD D NG I+A+EL+DVL+ LGEKCSL DC++MI +VDA
Sbjct: 64  FHCNDAGNDDS-KELRDAFDLYDVDKNGLITAKELHDVLRKLGEKCSLSDCRRMISNVDA 123

Query: 134 DGDGHVDFEEFKKMMA 150
           DGDG V+FEEFKKMMA
Sbjct: 124 DGDGSVNFEEFKKMMA 138

[Close]

BLAST of Spo24940.1 vs. NCBI nr
Match: gi|593800710|ref|XP_007163392.1| (hypothetical protein PHAVU_001G231000g [Phaseolus vulgaris])

HSP 1 Score: 178.7 bits (452), Expect = 7.500e-42
Identity = 88/135 (65.19%), Postives = 111/135 (82.22%), Query Frame = 1

		  
Query: 14  EIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDKDGDGYVDLRELEE 73
           E+  IF KFD NGDGKIS +EL+ L+ ALGS+T++EEV++MM E+D++GDGY+DL+E  E
Sbjct: 4   EVRKIFNKFDKNGDGKISCSELRELMAALGSKTTTEEVRRMMAELDRNGDGYIDLKEFGE 63

Query: 74  FHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCSLKDCKKMIQSVDA 133
           FH G GG  G  KELREAF LYD D NG ISA+EL+ V++ LGEKCSL DC++MI +VDA
Sbjct: 64  FHCGGGGGDG--KELREAFELYDLDKNGLISAKELHSVMRRLGEKCSLSDCRRMIGNVDA 123

Query: 134 DGDGHVDFEEFKKMM 149
           DGDG+V+FEEFKKMM
Sbjct: 124 DGDGNVNFEEFKKMM 136

[Close]

BLAST of Spo24940.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RAE4_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_093330 PE=4 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 1.400e-74
Identity = 149/150 (99.33%), Postives = 149/150 (99.33%), Query Frame = 1

		  
Query: 1   MAKNSNSQKKSTTEIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDK 60
           MAKNSNSQKKSTTEIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDK
Sbjct: 1   MAKNSNSQKKSTTEIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDK 60

Query: 61  DGDGYVDLRELEEFHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCS 120
           DGDGYVDLRE EEFHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCS
Sbjct: 61  DGDGYVDLREFEEFHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCS 120

Query: 121 LKDCKKMIQSVDADGDGHVDFEEFKKMMAA 151
           LKDCKKMIQSVDADGDGHVDFEEFKKMMAA
Sbjct: 121 LKDCKKMIQSVDADGDGHVDFEEFKKMMAA 150

[Close]

BLAST of Spo24940.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CYG9_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g027860 PE=4 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 2.700e-62
Identity = 130/150 (86.67%), Postives = 134/150 (89.33%), Query Frame = 1

		  
Query: 1   MAKNSNSQKKSTTEIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDK 60
           MAKNS        EIE IF KFDANGDGKISATEL+SLLQALGSETS+EEVKKMM+EMDK
Sbjct: 1   MAKNS--------EIERIFSKFDANGDGKISATELKSLLQALGSETSTEEVKKMMEEMDK 60

Query: 61  DGDGYVDLRELEEFHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCS 120
           DGDG+VDL E  EFHAGSGGNAGSTKEL EAF LYDKDGNGKISARELYDVLKSLGEKCS
Sbjct: 61  DGDGFVDLLEFSEFHAGSGGNAGSTKELEEAFRLYDKDGNGKISARELYDVLKSLGEKCS 120

Query: 121 LKDCKKMIQSVDADGDGHVDFEEFKKMMAA 151
           LKDCKKMI SVDADGDGHVDFEEFKKMMAA
Sbjct: 121 LKDCKKMISSVDADGDGHVDFEEFKKMMAA 142

[Close]

BLAST of Spo24940.1 vs. UniProtKB/TrEMBL
Match: I1JHX0_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_02G245700 PE=4 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 1.400e-42
Identity = 85/135 (62.96%), Postives = 111/135 (82.22%), Query Frame = 1

		  
Query: 14  EIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDKDGDGYVDLRELEE 73
           E+  IF KFD NGDGKIS TEL+ +L ALGS+T+ EE+K+MM+E+D++GDG++DL+E  +
Sbjct: 4   EVRQIFNKFDKNGDGKISVTELKDMLAALGSKTTDEELKRMMEELDQNGDGFIDLKEFAD 63

Query: 74  FHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCSLKDCKKMIQSVDA 133
           FH   G     +KELR+AF LYD D NG ISA+EL+DVL++LGEKCSL DC++MI +VDA
Sbjct: 64  FHCNGGAGKDDSKELRDAFDLYDVDKNGLISAKELHDVLRNLGEKCSLSDCRRMISNVDA 123

Query: 134 DGDGHVDFEEFKKMM 149
           DGDG+V+FEEFKKMM
Sbjct: 124 DGDGNVNFEEFKKMM 138

[Close]

BLAST of Spo24940.1 vs. UniProtKB/TrEMBL
Match: V7D2D5_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_001G231000g PE=4 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 5.200e-42
Identity = 88/135 (65.19%), Postives = 111/135 (82.22%), Query Frame = 1

		  
Query: 14  EIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDKDGDGYVDLRELEE 73
           E+  IF KFD NGDGKIS +EL+ L+ ALGS+T++EEV++MM E+D++GDGY+DL+E  E
Sbjct: 4   EVRKIFNKFDKNGDGKISCSELRELMAALGSKTTTEEVRRMMAELDRNGDGYIDLKEFGE 63

Query: 74  FHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCSLKDCKKMIQSVDA 133
           FH G GG  G  KELREAF LYD D NG ISA+EL+ V++ LGEKCSL DC++MI +VDA
Sbjct: 64  FHCGGGGGDG--KELREAFELYDLDKNGLISAKELHSVMRRLGEKCSLSDCRRMIGNVDA 123

Query: 134 DGDGHVDFEEFKKMM 149
           DGDG+V+FEEFKKMM
Sbjct: 124 DGDGNVNFEEFKKMM 136

[Close]

BLAST of Spo24940.1 vs. UniProtKB/TrEMBL
Match: A0A0L9UWX6_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan07g073800 PE=4 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 1.500e-41
Identity = 88/136 (64.71%), Postives = 109/136 (80.15%), Query Frame = 1

		  
Query: 14  EIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDKDGDGYVDLRELEE 73
           E+  IF KFD NGDGKIS TEL+ +L  LGS+T+ EE+K+MM+E+DK+GDGY+DL+E  +
Sbjct: 4   EVRQIFNKFDKNGDGKISITELRDMLGTLGSKTTKEELKRMMEELDKNGDGYIDLKEFAD 63

Query: 74  FHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCSLKDCKKMIQSVDA 133
           FH    GN  S KELR+AF LYD D NG I+A+EL+DVL  LGEKCSL DC++MI +VDA
Sbjct: 64  FHCNDAGNDDS-KELRDAFDLYDVDKNGLITAKELHDVLCKLGEKCSLSDCRRMISNVDA 123

Query: 134 DGDGHVDFEEFKKMMA 150
           DGDG V+FEEFKKMMA
Sbjct: 124 DGDGSVNFEEFKKMMA 138

[Close]

BLAST of Spo24940.1 vs. ExPASy Swiss-Prot
Match: CML18_ORYSJ (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica GN=CML18 PE=2 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 9.200e-40
Identity = 87/147 (59.18%), Postives = 107/147 (72.79%), Query Frame = 1

		  
Query: 14  EIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDKDGDGYVDLRELEE 73
           E+E +F+++DANGDGKISA EL S+L+ALG+     EV++MMDEMD D DG+VDL E   
Sbjct: 5   ELEQVFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEFAA 64

Query: 74  FHAGS----GGNAGSTK--------ELREAFSLYDKDGNGKISARELYDVLKSLGEKCSL 133
           FH G     GG  G  K        ELREAF +YD D NGKISAREL+ VL+ LG+KCS+
Sbjct: 65  FHCGPTPAHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGDKCSV 124

Query: 134 KDCKKMIQSVDADGDGHVDFEEFKKMM 149
            DC +MI+SVDADGDG V+F+EFKKMM
Sbjct: 125 ADCSRMIRSVDADGDGCVNFDEFKKMM 151

[Close]

BLAST of Spo24940.1 vs. ExPASy Swiss-Prot
Match: CML24_ARATH (Calcium-binding protein CML24 OS=Arabidopsis thaliana GN=CML24 PE=2 SV=2)

HSP 1 Score: 150.2 bits (378), Expect = 1.800e-35
Identity = 76/143 (53.15%), Postives = 100/143 (69.93%), Query Frame = 1

		  
Query: 11  STTEIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDKDGDGYVDLRE 70
           S  +I+ +FQ+FD NGDGKIS  EL+ +++AL    S EE   MM + D DG+G++DL E
Sbjct: 14  SMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDE 73

Query: 71  LEEFH----AGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCSLKDCKK 130
                     G G N     +L+EAF LYD DGNG+ISA+EL+ V+K+LGEKCS++DCKK
Sbjct: 74  FVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKK 133

Query: 131 MIQSVDADGDGHVDFEEFKKMMA 150
           MI  VD DGDG V+F+EFKKMM+
Sbjct: 134 MISKVDIDGDGCVNFDEFKKMMS 156

[Close]

BLAST of Spo24940.1 vs. ExPASy Swiss-Prot
Match: CML23_ARATH (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 9.800e-34
Identity = 78/151 (51.66%), Postives = 104/151 (68.87%), Query Frame = 1

		  
Query: 1   MAKN-SNSQKKSTTEIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMD 60
           M+KN S +   S  +I+ +FQ+FD N DGKIS  EL+ ++ AL    S EE K MM E D
Sbjct: 1   MSKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFD 60

Query: 61  KDGDGYVDLRELEEFH--AGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGE 120
            DG+G++DL E       +    N  + ++L+EAF LYD D NG+ISA EL+ V+K+LGE
Sbjct: 61  LDGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGE 120

Query: 121 KCSLKDCKKMIQSVDADGDGHVDFEEFKKMM 149
           KCS++DC++MI  VD+DGDG VDFEEFKKMM
Sbjct: 121 KCSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151

[Close]

BLAST of Spo24940.1 vs. ExPASy Swiss-Prot
Match: CML25_ARATH (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 1.700e-33
Identity = 71/146 (48.63%), Postives = 103/146 (70.55%), Query Frame = 1

		  
Query: 3   KNSNSQKKSTTEIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDKDG 62
           + + S K    E+E++F+KFD NGDGKIS+ EL +++ +LG E   EE++K + E+D+ G
Sbjct: 26  QKARSGKTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKG 85

Query: 63  DGYVDLRELEEFHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCSLK 122
           DGY++  E  E +          + L++AFS+YD DGNG ISA EL++VL+SLG++CS+ 
Sbjct: 86  DGYINFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIA 145

Query: 123 DCKKMIQSVDADGDGHVDFEEFKKMM 149
           +C+KMI  VD DGDG +DFEEFK MM
Sbjct: 146 ECRKMIGGVDKDGDGTIDFEEFKIMM 171

[Close]

BLAST of Spo24940.1 vs. ExPASy Swiss-Prot
Match: ALL8_OLEEU (Calcium-binding allergen Ole e 8 OS=Olea europaea PE=1 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 7.000e-32
Identity = 75/157 (47.77%), Postives = 107/157 (68.15%), Query Frame = 1

		  
Query: 1   MAKNSNSQKKSTT------EIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKM 60
           MA N++   K +       E++ +F +FDANGDGKIS  EL  +L+ALGS TS EE+ ++
Sbjct: 1   MAANTDRNSKPSVYLQEPNEVQGVFNRFDANGDGKISGDELAGVLKALGSNTSKEEIGRI 60

Query: 61  MDEMDKDGDGYVDLRELEEFHAGSGG---NAGSTKELREAFSLYDKDGNGKISARELYDV 120
           M+E+D D DG+++++E   F         ++G   EL+EAF LYD+D NG IS+ EL+ +
Sbjct: 61  MEEIDTDKDGFINVQEFAAFVKAETDPYPSSGGENELKEAFELYDQDHNGLISSVELHKI 120

Query: 121 LKSLGEKCSLKDCKKMIQSVDADGDGHVDFEEFKKMM 149
           L  LGE+ +  DC +MI+SVD+DGDG+V FEEFKKMM
Sbjct: 121 LTRLGERYAEHDCVEMIKSVDSDGDGYVSFEEFKKMM 157

[Close]

BLAST of Spo24940.1 vs. TAIR (Arabidopsis)
Match: AT5G37770.1 (EF hand calcium-binding protein family)

HSP 1 Score: 150.2 bits (378), Expect = 1.000e-36
Identity = 76/143 (53.15%), Postives = 100/143 (69.93%), Query Frame = 1

		  
Query: 11  STTEIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDKDGDGYVDLRE 70
           S  +I+ +FQ+FD NGDGKIS  EL+ +++AL    S EE   MM + D DG+G++DL E
Sbjct: 14  SMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDE 73

Query: 71  LEEFH----AGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCSLKDCKK 130
                     G G N     +L+EAF LYD DGNG+ISA+EL+ V+K+LGEKCS++DCKK
Sbjct: 74  FVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKK 133

Query: 131 MIQSVDADGDGHVDFEEFKKMMA 150
           MI  VD DGDG V+F+EFKKMM+
Sbjct: 134 MISKVDIDGDGCVNFDEFKKMMS 156

[Close]

BLAST of Spo24940.1 vs. TAIR (Arabidopsis)
Match: AT1G66400.1 (calmodulin like 23)

HSP 1 Score: 144.4 bits (363), Expect = 5.500e-35
Identity = 78/151 (51.66%), Postives = 104/151 (68.87%), Query Frame = 1

		  
Query: 1   MAKN-SNSQKKSTTEIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMD 60
           M+KN S +   S  +I+ +FQ+FD N DGKIS  EL+ ++ AL    S EE K MM E D
Sbjct: 1   MSKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFD 60

Query: 61  KDGDGYVDLRELEEFH--AGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGE 120
            DG+G++DL E       +    N  + ++L+EAF LYD D NG+ISA EL+ V+K+LGE
Sbjct: 61  LDGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGE 120

Query: 121 KCSLKDCKKMIQSVDADGDGHVDFEEFKKMM 149
           KCS++DC++MI  VD+DGDG VDFEEFKKMM
Sbjct: 121 KCSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151

[Close]

BLAST of Spo24940.1 vs. TAIR (Arabidopsis)
Match: AT1G24620.1 (EF hand calcium-binding protein family)

HSP 1 Score: 143.7 bits (361), Expect = 9.400e-35
Identity = 71/146 (48.63%), Postives = 103/146 (70.55%), Query Frame = 1

		  
Query: 3   KNSNSQKKSTTEIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDKDG 62
           + + S K    E+E++F+KFD NGDGKIS+ EL +++ +LG E   EE++K + E+D+ G
Sbjct: 26  QKARSGKTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKG 85

Query: 63  DGYVDLRELEEFHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCSLK 122
           DGY++  E  E +          + L++AFS+YD DGNG ISA EL++VL+SLG++CS+ 
Sbjct: 86  DGYINFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIA 145

Query: 123 DCKKMIQSVDADGDGHVDFEEFKKMM 149
           +C+KMI  VD DGDG +DFEEFK MM
Sbjct: 146 ECRKMIGGVDKDGDGTIDFEEFKIMM 171

[Close]

BLAST of Spo24940.1 vs. TAIR (Arabidopsis)
Match: AT1G18210.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 131.3 bits (329), Expect = 4.800e-31
Identity = 66/137 (48.18%), Postives = 95/137 (69.34%), Query Frame = 1

		  
Query: 14  EIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEMDKDGDGYVDLRELEE 73
           E++ +F +FD+NGDGKIS  EL  + +A+G+  +  E+ ++++E+D D DGY++L E   
Sbjct: 23  ELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFST 82

Query: 74  FHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEKCSLKDCKKMIQSVDA 133
               S     S  E+R+AF LYD+D NG ISA EL+ VL  LG  CS++DC +MI  VDA
Sbjct: 83  LCRSS----SSAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDA 142

Query: 134 DGDGHVDFEEFKKMMAA 151
           DGDG+V+FEEF+KMM +
Sbjct: 143 DGDGNVNFEEFQKMMTS 155

[Close]

BLAST of Spo24940.1 vs. TAIR (Arabidopsis)
Match: AT1G73630.1 (EF hand calcium-binding protein family)

HSP 1 Score: 129.4 bits (324), Expect = 1.800e-30
Identity = 70/152 (46.05%), Postives = 103/152 (67.76%), Query Frame = 1

		  
Query: 4   NSNSQKKSTT-----EIESIFQKFDANGDGKISATELQSLLQALGSETSSEEVKKMMDEM 63
           N  S  KSTT     E++ +F KFDANGDGKIS +EL ++ +++G+  + EE+ +++DE+
Sbjct: 5   NLESTNKSTTPSTDMELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEI 64

Query: 64  DKDGDGYVDLRELEEFHAGSGGNAGSTKELREAFSLYDKDGNGKISARELYDVLKSLGEK 123
           D D DG+++  E       S     S  E+REAF LYD++ NG IS+ E++ VL  LG  
Sbjct: 65  DIDCDGFINQEEFATICRSSS----SAVEIREAFDLYDQNKNGLISSSEIHKVLNRLGMT 124

Query: 124 CSLKDCKKMIQSVDADGDGHVDFEEFKKMMAA 151
           CS++DC +MI  VD DGDG+V+FEEF+KMM++
Sbjct: 125 CSVEDCVRMIGHVDTDGDGNVNFEEFQKMMSS 152

The following BLAST results are available for this feature:

BLAST of Spo24940.1 vs. NCBI nr
BLAST of Spo24940.1 vs. UniProtKB/TrEMBL
BLAST of Spo24940.1 vs. ExPASy Swiss-Prot
BLAST of Spo24940.1 vs. TAIR (Arabidopsis)

BLAST of Spo24940.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5

Position :

Zoom :

x 1


Match Name	E-value	Identity	Description
gi\|902208551\|gb\|KNA15979.1\|	2.0e-74	99.3	hypothetical protein SOVF_0933...	[more]
gi\|731316034\|ref\|XP_010693960.1\|	3.8e-62	86.6	PREDICTED: probable calcium-bi...	[more]
gi\|356501039\|ref\|XP_003519336.1\|	2.0e-42	62.9	PREDICTED: probable calcium-bi...	[more]
gi\|950987490\|ref\|XP_014503786.1\|	5.7e-42	64.7	PREDICTED: probable calcium-bi...	[more]
gi\|593800710\|ref\|XP_007163392.1\|	7.5e-42	65.1	hypothetical protein PHAVU_001...	[more]

BLAST of Spo24940.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5

Position :

Zoom :

x 1


Match Name	E-value	Identity	Description
A0A0K9RAE4_SPIOL	1.4e-74	99.3	Uncharacterized protein OS=Spi...	[more]
A0A0J8CYG9_BETVU	2.7e-62	86.6	Uncharacterized protein OS=Bet...	[more]
I1JHX0_SOYBN	1.4e-42	62.9	Uncharacterized protein OS=Gly...	[more]
V7D2D5_PHAVU	5.2e-42	65.1	Uncharacterized protein OS=Pha...	[more]
A0A0L9UWX6_PHAAN	1.5e-41	64.7	Uncharacterized protein OS=Pha...	[more]

BLAST of Spo24940.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5

Position :

Zoom :

x 1


Match Name	E-value	Identity	Description
CML18_ORYSJ	9.2e-40	59.1	Probable calcium-binding prote...	[more]
CML24_ARATH	1.8e-35	53.1	Calcium-binding protein CML24 ...	[more]
CML23_ARATH	9.8e-34	51.6	Probable calcium-binding prote...	[more]
CML25_ARATH	1.7e-33	48.6	Probable calcium-binding prote...	[more]
ALL8_OLEEU	7.0e-32	47.7	Calcium-binding allergen Ole e...	[more]

BLAST of Spo24940.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5

Position :

Zoom :

x 1


Match Name	E-value	Identity	Description
AT5G37770.1	1.0e-36	53.1	EF hand calcium-binding protei...	[more]
AT1G66400.1	5.5e-35	51.6	calmodulin like 23	[more]
AT1G24620.1	9.4e-35	48.6	EF hand calcium-binding protei...	[more]
AT1G18210.1	4.8e-31	48.1	Calcium-binding EF-hand family...	[more]
AT1G73630.1	1.8e-30	46.0	EF hand calcium-binding protei...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 86..148 score: 8.3E-13coord: 14..74 score: 5.6
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 87..115 score: 3.5E-7coord: 50..78 score: 6.7E-4coord: 123..150 score: 0.0021coord: 14..42 score: 4.
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 119..150 score: 12.7coord: 83..118 score: 15.741coord: 10..45 score: 15.406coord: 46..81 score: 14
IPR011992	EF-hand domain pair	GENE3D	1.10.238.10		coord: 6..148 score: 4.6
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 9..148 score: 4.43
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 96..108 score: -coord: 23..35 score: -coord: 132..144 score: -coord: 59..71 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 14..148 score: 8.6
None	No IPR available	PANTHER	PTHR10891:SF605	CALCIUM-BINDING PROTEIN CML25-RELATED	coord: 14..148 score: 8.6

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009862	systemic acquired resistance, salicylic acid mediated signaling pathway
biological_process	GO:0030001	metal ion transport
biological_process	GO:0006446	regulation of translational initiation
biological_process	GO:0006470	protein dephosphorylation
biological_process	GO:0050832	defense response to fungus
biological_process	GO:0009595	detection of biotic stimulus
biological_process	GO:0006468	protein phosphorylation
biological_process	GO:0006984	ER-nucleus signaling pathway
biological_process	GO:0055085	transmembrane transport
biological_process	GO:0006888	ER to Golgi vesicle-mediated transport
biological_process	GO:0009069	serine family amino acid metabolic process
biological_process	GO:0048767	root hair elongation
biological_process	GO:0010817	regulation of hormone levels
biological_process	GO:0000271	polysaccharide biosynthetic process
biological_process	GO:0045454	cell redox homeostasis
biological_process	GO:0009825	multidimensional cell growth
biological_process	GO:0019344	cysteine biosynthetic process
biological_process	GO:0071555	cell wall organization
biological_process	GO:0042742	defense response to bacterium
biological_process	GO:0015802	basic amino acid transport
biological_process	GO:0043481	anthocyanin accumulation in tissues in response to UV light
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0043069	negative regulation of programmed cell death
biological_process	GO:0006366	transcription by RNA polymerase II
biological_process	GO:0000394	RNA splicing, via endonucleolytic cleavage and ligation
biological_process	GO:0001101	response to acid chemical
biological_process	GO:0009987	cellular process
biological_process	GO:0044260	cellular macromolecule metabolic process
biological_process	GO:0044262	cellular carbohydrate metabolic process
biological_process	GO:0009725	response to hormone
biological_process	GO:0048193	Golgi vesicle transport
biological_process	GO:1901700	response to oxygen-containing compound
biological_process	GO:0006073	cellular glucan metabolic process
biological_process	GO:0030968	endoplasmic reticulum unfolded protein response
biological_process	GO:0010091	trichome branching
biological_process	GO:0043090	amino acid import
biological_process	GO:0015696	ammonium transport
biological_process	GO:0044237	cellular metabolic process
biological_process	GO:0065007	biological regulation
biological_process	GO:0009932	cell tip growth
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0000165	MAPK cascade
biological_process	GO:0042538	hyperosmotic salinity response
biological_process	GO:0009620	response to fungus
biological_process	GO:0010200	response to chitin
biological_process	GO:0010363	regulation of plant-type hypersensitive response
biological_process	GO:0031348	negative regulation of defense response
biological_process	GO:0006612	protein targeting to membrane
biological_process	GO:0009867	jasmonic acid mediated signaling pathway
biological_process	GO:0009695	jasmonic acid biosynthetic process
biological_process	GO:0052542	defense response by callose deposition
biological_process	GO:0034976	response to endoplasmic reticulum stress
biological_process	GO:0043269	regulation of ion transport
biological_process	GO:0009697	salicylic acid biosynthetic process
biological_process	GO:0009627	systemic acquired resistance
biological_process	GO:0002237	response to molecule of bacterial origin
biological_process	GO:0031347	regulation of defense response
biological_process	GO:0043900	regulation of multi-organism process
biological_process	GO:0010337	regulation of salicylic acid metabolic process
biological_process	GO:0002679	respiratory burst involved in defense response
biological_process	GO:0009414	response to water deprivation
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0009611	response to wounding
biological_process	GO:0009651	response to salt stress
biological_process	GO:0009755	hormone-mediated signaling pathway
biological_process	GO:0048519	negative regulation of biological process
biological_process	GO:0016310	phosphorylation
biological_process	GO:0098542	defense response to other organism
biological_process	GO:0009863	salicylic acid mediated signaling pathway
biological_process	GO:0043067	regulation of programmed cell death
biological_process	GO:0033554	cellular response to stress
biological_process	GO:0035556	intracellular signal transduction
biological_process	GO:1901701	cellular response to oxygen-containing compound
biological_process	GO:0071229	cellular response to acid chemical
biological_process	GO:0006464	cellular protein modification process
biological_process	GO:0006862	nucleotide transport
biological_process	GO:0008152	metabolic process
biological_process	GO:0010310	regulation of hydrogen peroxide metabolic process
biological_process	GO:0045087	innate immune response
cellular_component	GO:0008287	protein serine/threonine phosphatase complex
cellular_component	GO:0005736	DNA-directed RNA polymerase I complex
cellular_component	GO:0005665	DNA-directed RNA polymerase II, core complex
cellular_component	GO:0000419	DNA-directed RNA polymerase V complex
cellular_component	GO:0000418	DNA-directed RNA polymerase IV complex
cellular_component	GO:0005666	DNA-directed RNA polymerase III complex
cellular_component	GO:0016020	membrane
cellular_component	GO:0005618	cell wall
cellular_component	GO:0048046	apoplast
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0005840	ribosome
cellular_component	GO:0005774	vacuolar membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0015035	protein disulfide oxidoreductase activity
molecular_function	GO:0009055	electron transfer activity
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0051087	chaperone binding
molecular_function	GO:0016757	transferase activity, transferring glycosyl groups
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004722	protein serine/threonine phosphatase activity
molecular_function	GO:0016874	ligase activity
molecular_function	GO:0005515	protein binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity
molecular_function	GO:0004721	phosphoprotein phosphatase activity
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016301	kinase activity
molecular_function	GO:0016773	phosphotransferase activity, alcohol group as acceptor
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0004672	protein kinase activity
molecular_function	GO:0016740	transferase activity
molecular_function	GO:0050662	coenzyme binding
molecular_function	GO:0003743	translation initiation factor activity
molecular_function	GO:0004674	protein serine/threonine kinase activity
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function	GO:0016762	xyloglucan:xyloglucosyl transferase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0001054	RNA polymerase I activity
molecular_function	GO:0001055	RNA polymerase II activity
molecular_function	GO:0001056	RNA polymerase III activity
molecular_function	GO:0047918	GDP-mannose 3,5-epimerase activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo27165	0.80	Barchart \| Table
Spo23852	0.79	Barchart \| Table
Spo04379	0.77	Barchart \| Table
Spo05047	0.73	Barchart \| Table
Spo01226	0.73	Barchart \| Table
Spo08769	0.73	Barchart \| Table
Spo14295	0.72	Barchart \| Table
Spo17197	0.72	Barchart \| Table
Spo11150	0.71	Barchart \| Table
Spo13184	0.71	Barchart \| Table
Spo21785	0.71	Barchart \| Table
Spo22033	0.70	Barchart \| Table
Spo02554	0.70	Barchart \| Table
Spo00782	0.70	Barchart \| Table
Spo05496	0.69	Barchart \| Table
Spo01514	0.69	Barchart \| Table
Spo14902	0.69	Barchart \| Table
Spo04872	0.69	Barchart \| Table
Spo12884	0.69	Barchart \| Table
Spo13478	0.68	Barchart \| Table
Spo11975	0.68	Barchart \| Table
Spo19694	0.68	Barchart \| Table
Spo21878	0.68	Barchart \| Table
Spo21362	0.67	Barchart \| Table
Spo00611	0.67	Barchart \| Table
Spo05464	0.67	Barchart \| Table
Spo07646	0.67	Barchart \| Table
Spo08009	0.67	Barchart \| Table
Spo14667	0.67	Barchart \| Table
Spo18589	0.67	Barchart \| Table
Spo20380	0.67	Barchart \| Table
Spo24808	0.67	Barchart \| Table
Spo24818	0.67	Barchart \| Table
Spo15447	0.66	Barchart \| Table
Spo01137	0.66	Barchart \| Table
Spo05814	0.66	Barchart \| Table
Spo22397	0.66	Barchart \| Table
Spo23490	0.66	Barchart \| Table
Spo15639	0.66	Barchart \| Table
Spo18575	0.66	Barchart \| Table
Spo26790	0.66	Barchart \| Table
Spo21525	0.66	Barchart \| Table
Spo09635	0.66	Barchart \| Table
Spo20562	0.65	Barchart \| Table
Spo00396	0.65	Barchart \| Table
Spo24712	0.65	Barchart \| Table
Spo11776	0.65	Barchart \| Table
Spo18618	0.65	Barchart \| Table
Spo05781	0.65	Barchart \| Table