Spo25185 (gene)

Overview
NameSpo25185
Typegene
OrganismSpinacia oleracea (Spinach)
DescriptionBnaC03g00880D protein
LocationSuper_scaffold_70 : 1893961 .. 1894329 (-)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCTCCTCGACCTGCATATCAAGCTCTTCCGACTCCCGGTTTTCGAAACCGATGGATCTTGGTGGTGGTGGTGGTGGTGTAAGGGTGGTCGACAGCAAATCATGCCGGCAATTATACTTAAGAACTGCCTACACGTTTTCACGGCCGGATGAGGAGGAGGAGGAGGATGATGGGAATATGAAGAAGGCTAAGAAGTGCTTGGGTAGGGTTAGAAACAGAGTTTATAGAAGAGATGATCATCATAACAATAATAAGGGTAGTATGGTAATGTTTAGAAAGGTTAAGGAATTCTCTTGTGCTTCTCTCTTGGCTATGATTCGTAGGATGCTTGCTTGTACTGCAAAAGTTGATGCTGCTCACCATTAA

mRNA sequence

ATGTCCTCCTCGACCTGCATATCAAGCTCTTCCGACTCCCGGTTTTCGAAACCGATGGATCTTGGTGGTGGTGGTGGTGGTGTAAGGGTGGTCGACAGCAAATCATGCCGGCAATTATACTTAAGAACTGCCTACACGTTTTCACGGCCGGATGAGGAGGAGGAGGAGGATGATGGGAATATGAAGAAGGCTAAGAAGTGCTTGGGTAGGGTTAGAAACAGAGTTTATAGAAGAGATGATCATCATAACAATAATAAGGGTAGTATGGTAATGTTTAGAAAGGTTAAGGAATTCTCTTGTGCTTCTCTCTTGGCTATGATTCGTAGGATGCTTGCTTGTACTGCAAAAGTTGATGCTGCTCACCATTAA

Coding sequence (CDS)

ATGTCCTCCTCGACCTGCATATCAAGCTCTTCCGACTCCCGGTTTTCGAAACCGATGGATCTTGGTGGTGGTGGTGGTGGTGTAAGGGTGGTCGACAGCAAATCATGCCGGCAATTATACTTAAGAACTGCCTACACGTTTTCACGGCCGGATGAGGAGGAGGAGGAGGATGATGGGAATATGAAGAAGGCTAAGAAGTGCTTGGGTAGGGTTAGAAACAGAGTTTATAGAAGAGATGATCATCATAACAATAATAAGGGTAGTATGGTAATGTTTAGAAAGGTTAAGGAATTCTCTTGTGCTTCTCTCTTGGCTATGATTCGTAGGATGCTTGCTTGTACTGCAAAAGTTGATGCTGCTCACCATTAA

Protein sequence

MSSSTCISSSSDSRFSKPMDLGGGGGGVRVVDSKSCRQLYLRTAYTFSRPDEEEEEDDGNMKKAKKCLGRVRNRVYRRDDHHNNNKGSMVMFRKVKEFSCASLLAMIRRMLACTAKVDAAHH
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo25185.1Spo25185.1mRNA


Homology
[Close]
BLAST of Spo25185.1 vs. NCBI nr
Match: gi|902231363|gb|KNA22240.1| (hypothetical protein SOVF_035880 [Spinacia oleracea])

HSP 1 Score: 244.2 bits (622), Expect = 1.200e-61
Identity = 122/122 (100.00%), Postives = 122/122 (100.00%), Query Frame = 1 

		  

Query: 1   MSSSTCISSSSDSRFSKPMDLGGGGGGVRVVDSKSCRQLYLRTAYTFSRPDEEEEEDDGN 60
           MSSSTCISSSSDSRFSKPMDLGGGGGGVRVVDSKSCRQLYLRTAYTFSRPDEEEEEDDGN
Sbjct: 1   MSSSTCISSSSDSRFSKPMDLGGGGGGVRVVDSKSCRQLYLRTAYTFSRPDEEEEEDDGN 60

Query: 61  MKKAKKCLGRVRNRVYRRDDHHNNNKGSMVMFRKVKEFSCASLLAMIRRMLACTAKVDAA 120
           MKKAKKCLGRVRNRVYRRDDHHNNNKGSMVMFRKVKEFSCASLLAMIRRMLACTAKVDAA
Sbjct: 61  MKKAKKCLGRVRNRVYRRDDHHNNNKGSMVMFRKVKEFSCASLLAMIRRMLACTAKVDAA 120

Query: 121 HH 123
           HH
Sbjct: 121 HH 122
[Close]
BLAST of Spo25185.1 vs. NCBI nr
Match: gi|731349793|ref|XP_010686179.1| (PREDICTED: uncharacterized protein LOC104900461 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 149.1 bits (375), Expect = 5.200e-33
Identity = 88/141 (62.41%), Postives = 103/141 (73.05%), Query Frame = 1 

		  

Query: 1   MSSSTCISSS-------SDSRFSKPMDLGG-GGGGVRVVDSKSCRQLYLRTAYTFSRPDE 60
           MSSSTCISSS       S++RFS+P+D  G GGGGVRVVDSKSCRQ+YLRTAYTFSR   
Sbjct: 1   MSSSTCISSSNSNINPVSEARFSRPVDTDGCGGGGVRVVDSKSCRQMYLRTAYTFSR--- 60

Query: 61  EEEEDDGNMKKAKKCLGRVRNRV---YRRDD--------HHNNNKGSMVMFRKVKEFSCA 120
            E++ D  M+KAKKCLG+V++RV   Y+RD         +   N  SMV+F KVKEFSCA
Sbjct: 61  REDQTDNGMEKAKKCLGKVKDRVTLVYKRDHDKSFTIMRNSTTNNKSMVIFSKVKEFSCA 120

Query: 121 SLLAMIRRMLACTAKVDAAHH 123
           S L+MIRRMLACTAKVD A H
Sbjct: 121 SFLSMIRRMLACTAKVDVAEH 138
[Close]
BLAST of Spo25185.1 vs. NCBI nr
Match: gi|661889952|emb|CDP06672.1| (unnamed protein product [Coffea canephora])

HSP 1 Score: 81.3 bits (199), Expect = 1.300e-12
Identity = 51/129 (39.53%), Postives = 68/129 (52.71%), Query Frame = 1 

		  

Query: 11  SDSRFSKPMDLGGGGGGVR---------VVDSKSCRQLYLRTAYTFSRPDEEEEEDDGNM 70
           SD+ F K     GG G +          VVDS SCRQLYLR+AYTFSR       ++G  
Sbjct: 32  SDAEFVKSTGNLGGKGRLHNNYLHRSGAVVDSASCRQLYLRSAYTFSR-------EEGVQ 91

Query: 71  KKAKKCLGRVRNRVY--------RRDDHHNNNKGSMVMFRKVKEFSCASLLAMIRRMLAC 123
           ++ KKC GRV+ +V         R        +   VM R+VK+FSC +L  + +R+L+C
Sbjct: 92  ERTKKCFGRVKEKVAIARTATTSRFQPRRRKTRTRFVMVRRVKDFSCGALRTIFQRLLSC 151
[Close]
BLAST of Spo25185.1 vs. NCBI nr
Match: gi|565343878|ref|XP_006339051.1| (PREDICTED: uncharacterized protein LOC102602765 [Solanum tuberosum])

HSP 1 Score: 77.0 bits (188), Expect = 2.500e-11
Identity = 45/97 (46.39%), Postives = 56/97 (57.73%), Query Frame = 1 

		  

Query: 29  RVVDSKSCRQLYLRTAYTFSRPDEEEEEDDGNMKKAKKCLGRVRNRVYR---RDDHHNNN 88
           RVVDS SCRQLYLR+AYTFSR +   +       K KKCLG+VR RV     +       
Sbjct: 25  RVVDSVSCRQLYLRSAYTFSRKETVPQ-------KTKKCLGKVRERVANGKGKIGWRRRR 84

Query: 89  KGSMVMFRKVKEFSCASLLAMIRRMLACTAKVDAAHH 123
             S    R+VK+  CA+L  + R++LACTA VD   H
Sbjct: 85  VRSFTAVRRVKKICCAALCGVFRKLLACTATVDVVDH 114
[Close]
BLAST of Spo25185.1 vs. NCBI nr
Match: gi|802614805|ref|XP_012074847.1| (PREDICTED: uncharacterized protein LOC105636235 [Jatropha curcas])

HSP 1 Score: 76.6 bits (187), Expect = 3.300e-11
Identity = 50/115 (43.48%), Postives = 67/115 (58.26%), Query Frame = 1 

		  

Query: 11  SDSRFSKPMDLGGGG--GGVRVVDSKSCRQLYLRTAYTFSRPDEEEEEDDGNMKKAKKCL 70
           SD+ F K +   G G  G  RVVDS SCRQ+YLR+ YTFSR +   E       K KKC 
Sbjct: 33  SDAEFIKTVRRVGQGLHGQPRVVDSISCRQMYLRS-YTFSRKETVPE-------KTKKCF 92

Query: 71  GRVRNRV--YRRDDHHNNNKGSMVMFRKVKEFSCASLLAMIRRMLACTAKVDAAH 122
           GRV+ +V  + +       K   ++FRKVKEFSC +L  +  ++L+C A VD A+
Sbjct: 93  GRVKEKVTNHGKKRKEKGKKRKCLVFRKVKEFSCGALFRIFHKLLSCGASVDVAN 139
[Close]
BLAST of Spo25185.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RRS2_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_035880 PE=4 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 8.200e-62
Identity = 122/122 (100.00%), Postives = 122/122 (100.00%), Query Frame = 1 

		  

Query: 1   MSSSTCISSSSDSRFSKPMDLGGGGGGVRVVDSKSCRQLYLRTAYTFSRPDEEEEEDDGN 60
           MSSSTCISSSSDSRFSKPMDLGGGGGGVRVVDSKSCRQLYLRTAYTFSRPDEEEEEDDGN
Sbjct: 1   MSSSTCISSSSDSRFSKPMDLGGGGGGVRVVDSKSCRQLYLRTAYTFSRPDEEEEEDDGN 60

Query: 61  MKKAKKCLGRVRNRVYRRDDHHNNNKGSMVMFRKVKEFSCASLLAMIRRMLACTAKVDAA 120
           MKKAKKCLGRVRNRVYRRDDHHNNNKGSMVMFRKVKEFSCASLLAMIRRMLACTAKVDAA
Sbjct: 61  MKKAKKCLGRVRNRVYRRDDHHNNNKGSMVMFRKVKEFSCASLLAMIRRMLACTAKVDAA 120

Query: 121 HH 123
           HH
Sbjct: 121 HH 122
[Close]
BLAST of Spo25185.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BUT8_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_7g174700 PE=4 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 3.600e-33
Identity = 88/141 (62.41%), Postives = 103/141 (73.05%), Query Frame = 1 

		  

Query: 1   MSSSTCISSS-------SDSRFSKPMDLGG-GGGGVRVVDSKSCRQLYLRTAYTFSRPDE 60
           MSSSTCISSS       S++RFS+P+D  G GGGGVRVVDSKSCRQ+YLRTAYTFSR   
Sbjct: 1   MSSSTCISSSNSNINPVSEARFSRPVDTDGCGGGGVRVVDSKSCRQMYLRTAYTFSR--- 60

Query: 61  EEEEDDGNMKKAKKCLGRVRNRV---YRRDD--------HHNNNKGSMVMFRKVKEFSCA 120
            E++ D  M+KAKKCLG+V++RV   Y+RD         +   N  SMV+F KVKEFSCA
Sbjct: 61  REDQTDNGMEKAKKCLGKVKDRVTLVYKRDHDKSFTIMRNSTTNNKSMVIFSKVKEFSCA 120

Query: 121 SLLAMIRRMLACTAKVDAAHH 123
           S L+MIRRMLACTAKVD A H
Sbjct: 121 SFLSMIRRMLACTAKVDVAEH 138
[Close]
BLAST of Spo25185.1 vs. UniProtKB/TrEMBL
Match: A0A068UEK4_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00023600001 PE=4 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 9.200e-13
Identity = 51/129 (39.53%), Postives = 68/129 (52.71%), Query Frame = 1 

		  

Query: 11  SDSRFSKPMDLGGGGGGVR---------VVDSKSCRQLYLRTAYTFSRPDEEEEEDDGNM 70
           SD+ F K     GG G +          VVDS SCRQLYLR+AYTFSR       ++G  
Sbjct: 32  SDAEFVKSTGNLGGKGRLHNNYLHRSGAVVDSASCRQLYLRSAYTFSR-------EEGVQ 91

Query: 71  KKAKKCLGRVRNRVY--------RRDDHHNNNKGSMVMFRKVKEFSCASLLAMIRRMLAC 123
           ++ KKC GRV+ +V         R        +   VM R+VK+FSC +L  + +R+L+C
Sbjct: 92  ERTKKCFGRVKEKVAIARTATTSRFQPRRRKTRTRFVMVRRVKDFSCGALRTIFQRLLSC 151
[Close]
BLAST of Spo25185.1 vs. UniProtKB/TrEMBL
Match: M1ARK7_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400011054 PE=4 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 1.700e-11
Identity = 45/97 (46.39%), Postives = 56/97 (57.73%), Query Frame = 1 

		  

Query: 29  RVVDSKSCRQLYLRTAYTFSRPDEEEEEDDGNMKKAKKCLGRVRNRVYR---RDDHHNNN 88
           RVVDS SCRQLYLR+AYTFSR +   +       K KKCLG+VR RV     +       
Sbjct: 25  RVVDSVSCRQLYLRSAYTFSRKETVPQ-------KTKKCLGKVRERVANGKGKIGWRRRR 84

Query: 89  KGSMVMFRKVKEFSCASLLAMIRRMLACTAKVDAAHH 123
             S    R+VK+  CA+L  + R++LACTA VD   H
Sbjct: 85  VRSFTAVRRVKKICCAALCGVFRKLLACTATVDVVDH 114
[Close]
BLAST of Spo25185.1 vs. UniProtKB/TrEMBL
Match: A0A067KUN5_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_08997 PE=4 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 2.300e-11
Identity = 50/115 (43.48%), Postives = 67/115 (58.26%), Query Frame = 1 

		  

Query: 11  SDSRFSKPMDLGGGG--GGVRVVDSKSCRQLYLRTAYTFSRPDEEEEEDDGNMKKAKKCL 70
           SD+ F K +   G G  G  RVVDS SCRQ+YLR+ YTFSR +   E       K KKC 
Sbjct: 33  SDAEFIKTVRRVGQGLHGQPRVVDSISCRQMYLRS-YTFSRKETVPE-------KTKKCF 92

Query: 71  GRVRNRV--YRRDDHHNNNKGSMVMFRKVKEFSCASLLAMIRRMLACTAKVDAAH 122
           GRV+ +V  + +       K   ++FRKVKEFSC +L  +  ++L+C A VD A+
Sbjct: 93  GRVKEKVTNHGKKRKEKGKKRKCLVFRKVKEFSCGALFRIFHKLLSCGASVDVAN 139
[Close]
BLAST of Spo25185.1 vs. TAIR (Arabidopsis)
Match: AT5G02640.1 (unknown protein)

HSP 1 Score: 65.9 bits (159), Expect = 2.000e-11
Identity = 43/122 (35.25%), Postives = 62/122 (50.82%), Query Frame = 1 

		  

Query: 11  SDSRFSKPMDLGGGGGGVRVVDSKSCRQLYLRTAYTFSRPDEEEEEDDGNMKKAKKCLGR 70
           SD+ F + +  GGG    RVVDS SCRQ+YLR +YTFSR D+E + +  +      CLGR
Sbjct: 36  SDAEFVRSVRRGGGLPAARVVDSISCRQMYLR-SYTFSREDDESKSEKVSSGTQTSCLGR 95

Query: 71  VRNRVYRRDDHHNNN--------------KGSMVMFRKVKEFSCASLLAMIRRMLACTAK 119
           V+     R      N              +   V  +  +E +C+ +L + RR+L+C A 
Sbjct: 96  VKGTASSRRKSKEENGIIIVESTKPRRRGEKRRVSRKNKQEQACSVILRIFRRLLSCAAT 155
The following BLAST results are available for this feature:
BLAST of Spo25185.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902231363|gb|KNA22240.1|1.2e-61100.hypothetical protein SOVF_0358... [more]
gi|731349793|ref|XP_010686179.1|5.2e-3362.4PREDICTED: uncharacterized pro... [more]
gi|661889952|emb|CDP06672.1|1.3e-1239.5unnamed protein product [Coffe... [more]
gi|565343878|ref|XP_006339051.1|2.5e-1146.3PREDICTED: uncharacterized pro... [more]
gi|802614805|ref|XP_012074847.1|3.3e-1143.4PREDICTED: uncharacterized pro... [more]
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BLAST of Spo25185.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9RRS2_SPIOL8.2e-62100.Uncharacterized protein OS=Spi... [more]
A0A0J8BUT8_BETVU3.6e-3362.4Uncharacterized protein OS=Bet... [more]
A0A068UEK4_COFCA9.2e-1339.5Uncharacterized protein OS=Cof... [more]
M1ARK7_SOLTU1.7e-1146.3Uncharacterized protein OS=Sol... [more]
A0A067KUN5_JATCU2.3e-1143.4Uncharacterized protein OS=Jat... [more]
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BLAST of Spo25185.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Spo25185.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1
Match NameE-valueIdentityDescription
AT5G02640.12.0e-1135.2unknown protein[more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR35304FAMILY NOT NAMEDcoord: 19..122
score: 1.4

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009788 negative regulation of abscisic acid-activated signaling pathway
biological_process GO:0019761 glucosinolate biosynthetic process
biological_process GO:0009411 response to UV
biological_process GO:0045490 pectin catabolic process
biological_process GO:0009813 flavonoid biosynthetic process
biological_process GO:0042545 cell wall modification
biological_process GO:0008152 metabolic process
biological_process GO:0009966 regulation of signal transduction
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0055085 transmembrane transport
biological_process GO:0043132 NAD transport
biological_process GO:0006468 protein phosphorylation
biological_process GO:0015843 methylammonium transport
biological_process GO:0072488 ammonium transmembrane transport
biological_process GO:0006811 ion transport
biological_process GO:0010264 myo-inositol hexakisphosphate biosynthetic process
biological_process GO:0006855 drug transmembrane transport
biological_process GO:0005982 starch metabolic process
biological_process GO:0009069 serine family amino acid metabolic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0000272 polysaccharide catabolic process
biological_process GO:0042967 obsolete acyl-carrier-protein biosynthetic process
biological_process GO:0005985 sucrose metabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0031969 chloroplast membrane
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005618 cell wall
cellular_component GO:0005886 plasma membrane
cellular_component GO:0005737 cytoplasm
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0016740 transferase activity
molecular_function GO:0016829 lyase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0005506 iron ion binding
molecular_function GO:0043531 ADP binding
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0008080 N-acetyltransferase activity
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016161 beta-amylase activity
molecular_function GO:0051724 NAD transmembrane transporter activity
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0008519 ammonium transmembrane transporter activity
molecular_function GO:0015238 drug transmembrane transporter activity
molecular_function GO:0015297 antiporter activity
molecular_function GO:0030599 pectinesterase activity
RNA-Seq Expression