Spo25312 (gene) | SpinachBase

Overview


Name	Spo25312
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Tho complex subunit-like protein
Location	SpoScf_03555 : 8727 .. 10085 (-)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGCTTTCAGCTTCTTCAATGCTGAAAGGAAGAAGGGTTGTTGGCAAGGGAGAGGCTGTTGCTGCTCATTATGCCTTTGGTCCTCATGAAGATGATGCAATTATAAAGCACAGGCTTCTAACTCGCACAACCACAACAAGAGGTGAACCTCCATTGAAGAAGCTACAAAAGAAATTTACTGCCTTGGTCGTTGAGGTTGAGAAGAGTACAGATAATTACAATGATTGTGAGAAACTTGCAAAGGCCTTTTTGCAGGAACTTACCACTTTCGAGATTCCGTTATTGAAAAGCAAAGCAGTTGTTGATGCAAACTTGAGGGAAAAGGAGCATTTCAACGAGTTGAATGATGAGATAAATAGCCAGATTCTTGAGGTACAGACGGAAATTGAAGATCTAAAGAAAGAACTTGAAGCCAGCAAGATTGAGAGAAAACACAAGGAGGAGTGTGAGACGATACGGAAATTAATAGCCACGCAGCCTCCTAGGTCAGAAACAGAGAAGATGATAGCTGAACTTGAGAAAGAGATAGCTGCCTTGGAAGCAGAAGACATGGCTAGTGCAAGGCTGCTGGAACTTCGCAAGAAACAGTTTGCTCTGTTATTACATGTGGTATGTCTTAAATTTGATTCATTACTCGTTCGTTCTAATTTTGAGTTGTGACATGTAAGTCTGACTATACATTCTCAATGGATGTCAACTCCACATCATTATGCACAATTCTATCTGGGAAAAGTTGTTTTGCCGAACAAGTTAGGTTACTGAACCAAGCAACTTTTTAAATATGCCCTTAGTGGATCAAACTAGAGTTAGCTTTTGGTTAAGGAGTAAGGGAATGAATGCATGATAGACTTGTCGCTCCCTGTTAGGCATTTTGTTCTATTCCTCTAACTGTAAGTGCGATTTTGTAATGCAATTGGTTGAAGAGATAAAACATCTTTTGACTAATCATGTTTTGTGTAGGTGGATGAGTTGCAGGTGACAATAGAGGATGAACAAAGGAGTTTAGTGGAAGAGATGAAAACTCTTTCTGAAGAGCAGAAGGTTGGTCCAGAAGATCCAAGCGGGAGTGCAGAAGCCATGAACATTGATTAGCAATTTAGCAATAATTATGTAGTATTATGATCTGTGAATGATCCAACAATTACTGCTTTGCAGTATTAGAAAATCGATGTACAATTTGAATTTTGAAGTGCGAGTGATTTTTTTGATCTGTTCTTCAACGTTCGAAATTAATGAACTGCAGCTCTGAAGTAACAAAGGCAGATGTTTATGCTGCAAACATCAATACAGTTTCCTCAGATTGCTCTACACTTTAACTCATTAACGTACTTATTAACACAGTGATTGATTATATTT

mRNA sequence

ATGTTGCTTTCAGCTTCTTCAATGCTGAAAGGAAGAAGGGTTGTTGGCAAGGGAGAGGCTGTTGCTGCTCATTATGCCTTTGGTCCTCATGAAGATGATGCAATTATAAAGCACAGGCTTCTAACTCGCACAACCACAACAAGAGGTGAACCTCCATTGAAGAAGCTACAAAAGAAATTTACTGCCTTGGTCGTTGAGGTTGAGAAGAGTACAGATAATTACAATGATTGTGAGAAACTTGCAAAGGCCTTTTTGCAGGAACTTACCACTTTCGAGATTCCGTTATTGAAAAGCAAAGCAGTTGTTGATGCAAACTTGAGGGAAAAGGAGCATTTCAACGAGTTGAATGATGAGATAAATAGCCAGATTCTTGAGGTACAGACGGAAATTGAAGATCTAAAGAAAGAACTTGAAGCCAGCAAGATTGAGAGAAAACACAAGGAGGAGTGTGAGACGATACGGAAATTAATAGCCACGCAGCCTCCTAGGTCAGAAACAGAGAAGATGATAGCTGAACTTGAGAAAGAGATAGCTGCCTTGGAAGCAGAAGACATGGCTAGTGCAAGGCTGCTGGAACTTCGCAAGAAACAGTTTGCTCTGTTATTACATGTGGTGGATGAGTTGCAGGTGACAATAGAGGATGAACAAAGGAGTTTAGTGGAAGAGATGAAAACTCTTTCTGAAGAGCAGAAGGTTGGTCCAGAAGATCCAAGCGGGAGTGCAGAAGCCATGAACATTGATTAGCAATTTAGCAATAATTATGTAGTATTATGATCTGTGAATGATCCAACAATTACTGCTTTGCAGTATTAGAAAATCGATGTACAATTTGAATTTTGAAGTGCGAGTGATTTTTTTGATCTGTTCTTCAACGTTCGAAATTAATGAACTGCAGCTCTGAAGTAACAAAGGCAGATGTTTATGCTGCAAACATCAATACAGTTTCCTCAGATTGCTCTACACTTTAACTCATTAACGTACTTATTAACACAGTGATTGATTATATTT

Coding sequence (CDS)

ATGTTGCTTTCAGCTTCTTCAATGCTGAAAGGAAGAAGGGTTGTTGGCAAGGGAGAGGCTGTTGCTGCTCATTATGCCTTTGGTCCTCATGAAGATGATGCAATTATAAAGCACAGGCTTCTAACTCGCACAACCACAACAAGAGGTGAACCTCCATTGAAGAAGCTACAAAAGAAATTTACTGCCTTGGTCGTTGAGGTTGAGAAGAGTACAGATAATTACAATGATTGTGAGAAACTTGCAAAGGCCTTTTTGCAGGAACTTACCACTTTCGAGATTCCGTTATTGAAAAGCAAAGCAGTTGTTGATGCAAACTTGAGGGAAAAGGAGCATTTCAACGAGTTGAATGATGAGATAAATAGCCAGATTCTTGAGGTACAGACGGAAATTGAAGATCTAAAGAAAGAACTTGAAGCCAGCAAGATTGAGAGAAAACACAAGGAGGAGTGTGAGACGATACGGAAATTAATAGCCACGCAGCCTCCTAGGTCAGAAACAGAGAAGATGATAGCTGAACTTGAGAAAGAGATAGCTGCCTTGGAAGCAGAAGACATGGCTAGTGCAAGGCTGCTGGAACTTCGCAAGAAACAGTTTGCTCTGTTATTACATGTGGTGGATGAGTTGCAGGTGACAATAGAGGATGAACAAAGGAGTTTAGTGGAAGAGATGAAAACTCTTTCTGAAGAGCAGAAGGTTGGTCCAGAAGATCCAAGCGGGAGTGCAGAAGCCATGAACATTGATTAG

Protein sequence

MLLSASSMLKGRRVVGKGEAVAAHYAFGPHEDDAIIKHRLLTRTTTTRGEPPLKKLQKKFTALVVEVEKSTDNYNDCEKLAKAFLQELTTFEIPLLKSKAVVDANLREKEHFNELNDEINSQILEVQTEIEDLKKELEASKIERKHKEECETIRKLIATQPPRSETEKMIAELEKEIAALEAEDMASARLLELRKKQFALLLHVVDELQVTIEDEQRSLVEEMKTLSEEQKVGPEDPSGSAEAMNID

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo25312.1	Spo25312.1	mRNA

Homology

[Close]

BLAST of Spo25312.1 vs. NCBI nr
Match: gi|902163832|gb|KNA06890.1| (hypothetical protein SOVF_176910 [Spinacia oleracea])

HSP 1 Score: 446.0 bits (1146), Expect = 4.100e-122
Identity = 240/240 (100.00%), Postives = 240/240 (100.00%), Query Frame = 1


		  
Query: 8   MLKGRRVVGKGEAVAAHYAFGPHEDDAIIKHRLLTRTTTTRGEPPLKKLQKKFTALVVEV 67
           MLKGRRVVGKGEAVAAHYAFGPHEDDAIIKHRLLTRTTTTRGEPPLKKLQKKFTALVVEV
Sbjct: 1   MLKGRRVVGKGEAVAAHYAFGPHEDDAIIKHRLLTRTTTTRGEPPLKKLQKKFTALVVEV 60

Query: 68  EKSTDNYNDCEKLAKAFLQELTTFEIPLLKSKAVVDANLREKEHFNELNDEINSQILEVQ 127
           EKSTDNYNDCEKLAKAFLQELTTFEIPLLKSKAVVDANLREKEHFNELNDEINSQILEVQ
Sbjct: 61  EKSTDNYNDCEKLAKAFLQELTTFEIPLLKSKAVVDANLREKEHFNELNDEINSQILEVQ 120

Query: 128 TEIEDLKKELEASKIERKHKEECETIRKLIATQPPRSETEKMIAELEKEIAALEAEDMAS 187
           TEIEDLKKELEASKIERKHKEECETIRKLIATQPPRSETEKMIAELEKEIAALEAEDMAS
Sbjct: 121 TEIEDLKKELEASKIERKHKEECETIRKLIATQPPRSETEKMIAELEKEIAALEAEDMAS 180

Query: 188 ARLLELRKKQFALLLHVVDELQVTIEDEQRSLVEEMKTLSEEQKVGPEDPSGSAEAMNID 247
           ARLLELRKKQFALLLHVVDELQVTIEDEQRSLVEEMKTLSEEQKVGPEDPSGSAEAMNID
Sbjct: 181 ARLLELRKKQFALLLHVVDELQVTIEDEQRSLVEEMKTLSEEQKVGPEDPSGSAEAMNID 240

[Close]

BLAST of Spo25312.1 vs. NCBI nr
Match: gi|731339190|ref|XP_010680719.1| (PREDICTED: THO complex subunit 7A-like isoform X1 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 384.4 bits (986), Expect = 1.500e-103
Identity = 202/240 (84.17%), Postives = 218/240 (90.83%), Query Frame = 1


		  
Query: 8   MLKGRRVVGKGEAVAAHYAFGPHEDDAIIKHRLLTRTTTTRGEPPLKKLQKKFTALVVEV 67
           MLK R+VVGKGEA+AAHYAF PHEDD IIKHRLLTRTTTTRGEPPLKKLQKKFTA  +E+
Sbjct: 1   MLKARKVVGKGEAMAAHYAFSPHEDDVIIKHRLLTRTTTTRGEPPLKKLQKKFTAFALEI 60

Query: 68  EKSTDNYNDCEKLAKAFLQELTTFEIPLLKSKAVVDANLREKEHFNELNDEINSQILEVQ 127
           EK  DNYNDCE+LAKAFLQEL TFEIPL KSK V+DAN+REKEHFNELNDEINSQIL+VQ
Sbjct: 61  EKDADNYNDCERLAKAFLQELNTFEIPLWKSKVVIDANVREKEHFNELNDEINSQILQVQ 120

Query: 128 TEIEDLKKELEASKIERKHKEECETIRKLIATQPPRSETEKMIAELEKEIAALEAEDMAS 187
            EIEDLKKELEASKIERKHKEECE IRK+IA Q PRSETE+MIA LEKEIAALEAED AS
Sbjct: 121 AEIEDLKKELEASKIERKHKEECEAIRKIIAMQRPRSETERMIAALEKEIAALEAEDTAS 180

Query: 188 ARLLELRKKQFALLLHVVDELQVTIEDEQRSLVEEMKTLSEEQKVGPEDPSGSAEAMNID 247
           +RLLELRKKQFALLLHVVDELQ+TIEDEQ+SL EEMK LS+EQKVGPED +  A AMN+D
Sbjct: 181 SRLLELRKKQFALLLHVVDELQMTIEDEQKSLAEEMKNLSDEQKVGPEDMNSGAAAMNVD 240

[Close]

BLAST of Spo25312.1 vs. NCBI nr
Match: gi|731339467|ref|XP_010680871.1| (PREDICTED: uncharacterized protein LOC104895923 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 379.0 bits (972), Expect = 6.200e-102
Identity = 200/245 (81.63%), Postives = 219/245 (89.39%), Query Frame = 1


		  
Query: 2   LLSASSMLKGRRVVGKGEAVAAHYAFGPHEDDAIIKHRLLTRTTTTRGEPPLKKLQKKFT 61
           +L+ +   K R+VVGKGEA+AAHYAF PHEDD IIKHRLLTRTTTTRGEPPLKKLQKKFT
Sbjct: 378 VLTCTVKYKARKVVGKGEAMAAHYAFSPHEDDVIIKHRLLTRTTTTRGEPPLKKLQKKFT 437

Query: 62  ALVVEVEKSTDNYNDCEKLAKAFLQELTTFEIPLLKSKAVVDANLREKEHFNELNDEINS 121
           A  +E+EK  DNYNDCE+LAKAFLQEL TFEIPL KSK V+DAN+REKEHFNELNDEINS
Sbjct: 438 AFALEIEKDADNYNDCERLAKAFLQELNTFEIPLWKSKVVIDANVREKEHFNELNDEINS 497

Query: 122 QILEVQTEIEDLKKELEASKIERKHKEECETIRKLIATQPPRSETEKMIAELEKEIAALE 181
           QIL+VQ EIEDLKKELEASKIERKHKEECE IRK+IA Q PRSETE+MIA LEKEIAALE
Sbjct: 498 QILQVQAEIEDLKKELEASKIERKHKEECEAIRKIIAMQRPRSETERMIAALEKEIAALE 557

Query: 182 AEDMASARLLELRKKQFALLLHVVDELQVTIEDEQRSLVEEMKTLSEEQKVGPEDPSGSA 241
           AED AS+RLLELRKKQFALLLHVVDELQ+TIEDEQ+SL EEMK LS+EQKVGPED +  A
Sbjct: 558 AEDTASSRLLELRKKQFALLLHVVDELQMTIEDEQKSLAEEMKNLSDEQKVGPEDMNSGA 617

Query: 242 EAMNI 247
            AMN+
Sbjct: 618 AAMNL 622

[Close]

BLAST of Spo25312.1 vs. NCBI nr
Match: gi|731339196|ref|XP_010680722.1| (PREDICTED: THO complex subunit 7A-like isoform X2 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 364.4 bits (934), Expect = 1.600e-97
Identity = 192/224 (85.71%), Postives = 206/224 (91.96%), Query Frame = 1


		  
Query: 8   MLKGRRVVGKGEAVAAHYAFGPHEDDAIIKHRLLTRTTTTRGEPPLKKLQKKFTALVVEV 67
           MLK R+VVGKGEA+AAHYAF PHEDD IIKHRLLTRTTTTRGEPPLKKLQKKFTA  +E+
Sbjct: 1   MLKARKVVGKGEAMAAHYAFSPHEDDVIIKHRLLTRTTTTRGEPPLKKLQKKFTAFALEI 60

Query: 68  EKSTDNYNDCEKLAKAFLQELTTFEIPLLKSKAVVDANLREKEHFNELNDEINSQILEVQ 127
           EK  DNYNDCE+LAKAFLQEL TFEIPL KSK V+DAN+REKEHFNELNDEINSQIL+VQ
Sbjct: 61  EKDADNYNDCERLAKAFLQELNTFEIPLWKSKVVIDANVREKEHFNELNDEINSQILQVQ 120

Query: 128 TEIEDLKKELEASKIERKHKEECETIRKLIATQPPRSETEKMIAELEKEIAALEAEDMAS 187
            EIEDLKKELEASKIERKHKEECE IRK+IA Q PRSETE+MIA LEKEIAALEAED AS
Sbjct: 121 AEIEDLKKELEASKIERKHKEECEAIRKIIAMQRPRSETERMIAALEKEIAALEAEDTAS 180

Query: 188 ARLLELRKKQFALLLHVVDELQVTIEDEQRSLVEEMKTLSEEQK 232
           +RLLELRKKQFALLLHVVDELQ+TIEDEQ+SL EEMK LS+EQK
Sbjct: 181 SRLLELRKKQFALLLHVVDELQMTIEDEQKSLAEEMKNLSDEQK 224

[Close]

BLAST of Spo25312.1 vs. NCBI nr
Match: gi|661886750|emb|CDP09699.1| (unnamed protein product [Coffea canephora])

HSP 1 Score: 349.0 bits (894), Expect = 6.900e-93
Identity = 184/240 (76.67%), Postives = 210/240 (87.50%), Query Frame = 1


		  
Query: 8   MLKGRRVVGKGEAVAAHYAFGPHEDDAIIKHRLLTRTTTTRGEPPLKKLQKKFTALVVEV 67
           ++KGR+V GKGEAVAAHYAFGP EDD IIKHRLLTRTTTTRGEPPLKKLQKKFTA  +EV
Sbjct: 2   LVKGRKVAGKGEAVAAHYAFGPLEDDIIIKHRLLTRTTTTRGEPPLKKLQKKFTAFALEV 61

Query: 68  EKSTDNYNDCEKLAKAFLQELTTFEIPLLKSKAVVDANLREKEHFNELNDEINSQILEVQ 127
           EK  DNY DCE+LAKAFLQEL TFEIPLLKSKAV+DAN+REKE+FNEL D+IN QIL+ Q
Sbjct: 62  EKEGDNYGDCERLAKAFLQELNTFEIPLLKSKAVIDANVREKENFNELKDDINKQILQAQ 121

Query: 128 TEIEDLKKELEASKIERKHKEECETIRKLIATQPPRSETEKMIAELEKEIAALEAEDMAS 187
            +IEDLKK+LEASKIER+HKEECE IR+LIA QPPRSET+K+I+ELE+EI  LEAE+ A 
Sbjct: 122 DDIEDLKKQLEASKIERQHKEECEAIRRLIALQPPRSETQKVISELEREIELLEAENTAG 181

Query: 188 ARLLELRKKQFALLLHVVDELQVTIEDEQRSLVEEMKTLSEEQKVGPEDPSGSAEAMNID 247
           +R LELRKKQFALLLHVVDELQ TIE+EQRSLVEEM+ + +E K G ED +G  EAM +D
Sbjct: 182 SRTLELRKKQFALLLHVVDELQNTIEEEQRSLVEEMRMVIDEHKNGVEDANGGPEAMTVD 241

[Close]

BLAST of Spo25312.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QI28_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_176910 PE=4 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 2.900e-122
Identity = 240/240 (100.00%), Postives = 240/240 (100.00%), Query Frame = 1


		  
Query: 8   MLKGRRVVGKGEAVAAHYAFGPHEDDAIIKHRLLTRTTTTRGEPPLKKLQKKFTALVVEV 67
           MLKGRRVVGKGEAVAAHYAFGPHEDDAIIKHRLLTRTTTTRGEPPLKKLQKKFTALVVEV
Sbjct: 1   MLKGRRVVGKGEAVAAHYAFGPHEDDAIIKHRLLTRTTTTRGEPPLKKLQKKFTALVVEV 60

Query: 68  EKSTDNYNDCEKLAKAFLQELTTFEIPLLKSKAVVDANLREKEHFNELNDEINSQILEVQ 127
           EKSTDNYNDCEKLAKAFLQELTTFEIPLLKSKAVVDANLREKEHFNELNDEINSQILEVQ
Sbjct: 61  EKSTDNYNDCEKLAKAFLQELTTFEIPLLKSKAVVDANLREKEHFNELNDEINSQILEVQ 120

Query: 128 TEIEDLKKELEASKIERKHKEECETIRKLIATQPPRSETEKMIAELEKEIAALEAEDMAS 187
           TEIEDLKKELEASKIERKHKEECETIRKLIATQPPRSETEKMIAELEKEIAALEAEDMAS
Sbjct: 121 TEIEDLKKELEASKIERKHKEECETIRKLIATQPPRSETEKMIAELEKEIAALEAEDMAS 180

Query: 188 ARLLELRKKQFALLLHVVDELQVTIEDEQRSLVEEMKTLSEEQKVGPEDPSGSAEAMNID 247
           ARLLELRKKQFALLLHVVDELQVTIEDEQRSLVEEMKTLSEEQKVGPEDPSGSAEAMNID
Sbjct: 181 ARLLELRKKQFALLLHVVDELQVTIEDEQRSLVEEMKTLSEEQKVGPEDPSGSAEAMNID 240

[Close]

BLAST of Spo25312.1 vs. UniProtKB/TrEMBL
Match: A0A0J8F0B3_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_6g136790 PE=4 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 1.000e-103
Identity = 202/240 (84.17%), Postives = 218/240 (90.83%), Query Frame = 1


		  
Query: 8   MLKGRRVVGKGEAVAAHYAFGPHEDDAIIKHRLLTRTTTTRGEPPLKKLQKKFTALVVEV 67
           MLK R+VVGKGEA+AAHYAF PHEDD IIKHRLLTRTTTTRGEPPLKKLQKKFTA  +E+
Sbjct: 1   MLKARKVVGKGEAMAAHYAFSPHEDDVIIKHRLLTRTTTTRGEPPLKKLQKKFTAFALEI 60

Query: 68  EKSTDNYNDCEKLAKAFLQELTTFEIPLLKSKAVVDANLREKEHFNELNDEINSQILEVQ 127
           EK  DNYNDCE+LAKAFLQEL TFEIPL KSK V+DAN+REKEHFNELNDEINSQIL+VQ
Sbjct: 61  EKDADNYNDCERLAKAFLQELNTFEIPLWKSKVVIDANVREKEHFNELNDEINSQILQVQ 120

Query: 128 TEIEDLKKELEASKIERKHKEECETIRKLIATQPPRSETEKMIAELEKEIAALEAEDMAS 187
            EIEDLKKELEASKIERKHKEECE IRK+IA Q PRSETE+MIA LEKEIAALEAED AS
Sbjct: 121 AEIEDLKKELEASKIERKHKEECEAIRKIIAMQRPRSETERMIAALEKEIAALEAEDTAS 180

Query: 188 ARLLELRKKQFALLLHVVDELQVTIEDEQRSLVEEMKTLSEEQKVGPEDPSGSAEAMNID 247
           +RLLELRKKQFALLLHVVDELQ+TIEDEQ+SL EEMK LS+EQKVGPED +  A AMN+D
Sbjct: 181 SRLLELRKKQFALLLHVVDELQMTIEDEQKSLAEEMKNLSDEQKVGPEDMNSGAAAMNVD 240

[Close]

BLAST of Spo25312.1 vs. UniProtKB/TrEMBL
Match: A0A068UNB5_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00030133001 PE=4 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 4.800e-93
Identity = 184/240 (76.67%), Postives = 210/240 (87.50%), Query Frame = 1


		  
Query: 8   MLKGRRVVGKGEAVAAHYAFGPHEDDAIIKHRLLTRTTTTRGEPPLKKLQKKFTALVVEV 67
           ++KGR+V GKGEAVAAHYAFGP EDD IIKHRLLTRTTTTRGEPPLKKLQKKFTA  +EV
Sbjct: 2   LVKGRKVAGKGEAVAAHYAFGPLEDDIIIKHRLLTRTTTTRGEPPLKKLQKKFTAFALEV 61

Query: 68  EKSTDNYNDCEKLAKAFLQELTTFEIPLLKSKAVVDANLREKEHFNELNDEINSQILEVQ 127
           EK  DNY DCE+LAKAFLQEL TFEIPLLKSKAV+DAN+REKE+FNEL D+IN QIL+ Q
Sbjct: 62  EKEGDNYGDCERLAKAFLQELNTFEIPLLKSKAVIDANVREKENFNELKDDINKQILQAQ 121

Query: 128 TEIEDLKKELEASKIERKHKEECETIRKLIATQPPRSETEKMIAELEKEIAALEAEDMAS 187
            +IEDLKK+LEASKIER+HKEECE IR+LIA QPPRSET+K+I+ELE+EI  LEAE+ A 
Sbjct: 122 DDIEDLKKQLEASKIERQHKEECEAIRRLIALQPPRSETQKVISELEREIELLEAENTAG 181

Query: 188 ARLLELRKKQFALLLHVVDELQVTIEDEQRSLVEEMKTLSEEQKVGPEDPSGSAEAMNID 247
           +R LELRKKQFALLLHVVDELQ TIE+EQRSLVEEM+ + +E K G ED +G  EAM +D
Sbjct: 182 SRTLELRKKQFALLLHVVDELQNTIEEEQRSLVEEMRMVIDEHKNGVEDANGGPEAMTVD 241

[Close]

BLAST of Spo25312.1 vs. UniProtKB/TrEMBL
Match: A0A061F6H5_THECC (Tho complex subunit 7/Mft1p OS=Theobroma cacao GN=TCM_025487 PE=4 SV=1)

HSP 1 Score: 347.4 bits (890), Expect = 1.400e-92
Identity = 183/238 (76.89%), Postives = 211/238 (88.66%), Query Frame = 1


		  
Query: 10  KGRRVVGKGEAVAAHYAFGPHEDDAIIKHRLLTRTTTTRGEPPLKKLQKKFTALVVEVEK 69
           KGR+V  +GE VAA+YAFGP EDD IIKHRLLTRTTTTRGEPPLKKLQKKFT+ V+EVEK
Sbjct: 4   KGRKVSSRGETVAANYAFGPAEDDIIIKHRLLTRTTTTRGEPPLKKLQKKFTSFVLEVEK 63

Query: 70  STDNYNDCEKLAKAFLQELTTFEIPLLKSKAVVDANLREKEHFNELNDEINSQILEVQTE 129
             DNYN+C KL+K+FLQEL+TFEIPLLKSKAV+DANLREKE+FNEL DEIN QIL+ QT+
Sbjct: 64  DEDNYNECAKLSKSFLQELSTFEIPLLKSKAVIDANLREKENFNELKDEINRQILQAQTD 123

Query: 130 IEDLKKELEASKIERKHKEECETIRKLIATQPPRSETEKMIAELEKEIAALEAEDMASAR 189
           IEDLKK+LE SKIER+HKEECE IRKLI+ QPPRSET+K I ELEKEIAA EAE+ A +R
Sbjct: 124 IEDLKKQLEESKIERQHKEECEAIRKLISAQPPRSETQKSITELEKEIAAWEAENTAGSR 183

Query: 190 LLELRKKQFALLLHVVDELQVTIEDEQRSLVEEMKTLSEEQKVGPEDPSGSAEAMNID 248
           LLELRKKQFALLLHVVDELQ TIE+EQ+SL+EEM+ ++EEQK G ED +G +EAM +D
Sbjct: 184 LLELRKKQFALLLHVVDELQNTIEEEQKSLIEEMRMVTEEQKSGMEDANGGSEAMAVD 241

[Close]

BLAST of Spo25312.1 vs. UniProtKB/TrEMBL
Match: A0A0B0PUA3_GOSAR (THO complex subunit 7 OS=Gossypium arboreum GN=F383_12023 PE=4 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 1.800e-92
Identity = 183/238 (76.89%), Postives = 212/238 (89.08%), Query Frame = 1


		  
Query: 10  KGRRVVGKGEAVAAHYAFGPHEDDAIIKHRLLTRTTTTRGEPPLKKLQKKFTALVVEVEK 69
           KGR+V G+GE V A+YAFGP EDDAIIKHRLLTRTTTTRGEPPLKKLQ+KFT+ V+EVEK
Sbjct: 4   KGRKVSGRGETVTANYAFGPAEDDAIIKHRLLTRTTTTRGEPPLKKLQRKFTSFVLEVEK 63

Query: 70  STDNYNDCEKLAKAFLQELTTFEIPLLKSKAVVDANLREKEHFNELNDEINSQILEVQTE 129
             +NYNDC KL+KAFLQEL+TFEIPLLKSKAV+DANLREKE+FNEL DEIN QI  VQT+
Sbjct: 64  DEENYNDCAKLSKAFLQELSTFEIPLLKSKAVIDANLREKENFNELKDEINRQISLVQTD 123

Query: 130 IEDLKKELEASKIERKHKEECETIRKLIATQPPRSETEKMIAELEKEIAALEAEDMASAR 189
           IE+LKK+LE SKIER+HKEECE IRKLI+ QPPRSET+K I E+EKEIAALEAE+ A +R
Sbjct: 124 IEELKKQLEESKIERQHKEECEAIRKLISAQPPRSETQKSIIEIEKEIAALEAENTAGSR 183

Query: 190 LLELRKKQFALLLHVVDELQVTIEDEQRSLVEEMKTLSEEQKVGPEDPSGSAEAMNID 248
           LLELRKKQFALLLHVVDELQ TIE++Q+SL+EEM+ ++EEQK G ED SG +EAM +D
Sbjct: 184 LLELRKKQFALLLHVVDELQNTIEEDQKSLIEEMRMVTEEQKSGMEDASGGSEAMAVD 241

[Close]

BLAST of Spo25312.1 vs. ExPASy Swiss-Prot
Match: THO7B_ARATH (THO complex subunit 7B OS=Arabidopsis thaliana GN=THO7B PE=1 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 1.100e-79
Identity = 163/241 (67.63%), Postives = 199/241 (82.57%), Query Frame = 1


		  
Query: 9   LKGRRVVGKGEAVAA--HYAFGPHEDDAIIKHRLLTRTTTTRGEPPLKKLQKKFTALVVE 68
           +K RR+ G+ E V    +YAF P +DD II++RLLTRTTTTRGEPPLKKLQKKFT+ V+E
Sbjct: 3   VKARRISGRLETVVTKVNYAFDPVDDDKIIRNRLLTRTTTTRGEPPLKKLQKKFTSFVLE 62

Query: 69  VEKSTDNYNDCEKLAKAFLQELTTFEIPLLKSKAVVDANLREKEHFNELNDEINSQILEV 128
           V+K  +NYNDC +LAKAFLQEL+TFEIPLLKS+AVV+ANLREKE FNE+ DE   QI++ 
Sbjct: 63  VDKEEENYNDCGRLAKAFLQELSTFEIPLLKSQAVVEANLREKESFNEVKDETERQIMQA 122

Query: 129 QTEIEDLKKELEASKIERKHKEECETIRKLIATQPPRSETEKMIAELEKEIAALEAEDMA 188
           + EIEDLKK+LE SKI+R+HKEECETIRKLI+ QPPRSETEK+I EL KEIA LEAE  A
Sbjct: 123 KAEIEDLKKQLEESKIDRQHKEECETIRKLISAQPPRSETEKVIYELNKEIAELEAESTA 182

Query: 189 SARLLELRKKQFALLLHVVDELQVTIEDEQRSLVEEMKTLSEEQKVGPEDPSGSAEAMNI 248
           S RLLELRKKQFALL+HVVDELQ T+EDEQ+SLV+E+++ SE+Q+          +AM++
Sbjct: 183 SWRLLELRKKQFALLMHVVDELQNTMEDEQKSLVDEIRSASEDQR-------NITDAMSV 236

[Close]

BLAST of Spo25312.1 vs. ExPASy Swiss-Prot
Match: THO7A_ARATH (THO complex subunit 7A OS=Arabidopsis thaliana GN=THO7A PE=1 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 2.800e-78
Identity = 163/240 (67.92%), Postives = 196/240 (81.67%), Query Frame = 1


		  
Query: 9   LKGRRVV-GKGEAVAAHYAFGPHEDDAIIKHRLLTRTTTTRGEPPLKKLQKKFTALVVEV 68
           ++ RR+V G+ E VAA+YAF P  DD II++RLLTRTTTTRGEPPLKKLQKKFT+ V+E+
Sbjct: 3   VRARRIVSGRSETVAANYAFDPLHDDKIIRNRLLTRTTTTRGEPPLKKLQKKFTSFVIEL 62

Query: 69  EKSTDNYNDCEKLAKAFLQELTTFEIPLLKSKAVVDANLREKEHFNELNDEINSQILEVQ 128
           +K  DNY++C +LAKAFLQEL+ FEIPLLKS+ VV ANLREKE+FNEL DE N QI++ Q
Sbjct: 63  DKEEDNYSECGRLAKAFLQELSAFEIPLLKSQVVVAANLREKENFNELKDETNRQIMQAQ 122

Query: 129 TEIEDLKKELEASKIERKHKEECETIRKLIATQPPRSETEKMIAELEKEIAALEAEDMAS 188
            +IEDLKK+LE SKIER+ KEECE IRKLI+ QPPRSET+K+I EL+KEIA LEAE+ AS
Sbjct: 123 ADIEDLKKQLEESKIERQQKEECEAIRKLISAQPPRSETQKVIHELKKEIAELEAENTAS 182

Query: 189 ARLLELRKKQFALLLHVVDELQVTIEDEQRSLVEEMKTLSEEQKVGPEDPSGSAEAMNID 248
            RLLELRKKQFALLLHVVDELQ T+EDEQ+S+VEEM+             +  AEAM+ID
Sbjct: 183 WRLLELRKKQFALLLHVVDELQETMEDEQKSMVEEMQR---------NITADGAEAMSID 233

[Close]

BLAST of Spo25312.1 vs. ExPASy Swiss-Prot
Match: THOC7_NEMVE (THO complex subunit 7 homolog OS=Nematostella vectensis GN=thoc7 PE=3 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 1.900e-13
Identity = 66/201 (32.84%), Postives = 102/201 (50.75%), Query Frame = 1


		  
Query: 32  DDAIIKHRLLTRTTTTRGEPPLKKLQKKFTALVVEVEKSTDNYNDCEKLAKAFLQELTTF 91
           DD +I+ RLL        +  +  L K F    V+   STD+ ND     +  L +L   
Sbjct: 3   DDEVIRKRLLIDGEGVGDDRRITTLMKTF----VKWCNSTDDDNDASY--QKLLAQLAQC 62

Query: 92  EIPLLKSKAVVDANLREKEHFNELNDEINSQILEVQTEIEDLKKELEASKIERKHKEECE 151
           E  + K++ V   N RE E++ +   E+   I     EI   K  L+ +K  R++K+E +
Sbjct: 63  EFAMGKTEMVHAMNERETENYEKAYKEVEQSITHAYEEIATCKTNLQEAKRIRRNKQEYD 122

Query: 152 TIRKLIATQPPRSETEKMIAELEKEIAALEAEDMASARLLELRKKQFALLLHVVDELQVT 211
            + K I   P R ET + I+ELEK++ +L     +    LELRKKQF LL++ + ELQ  
Sbjct: 123 ALAKEITKHPERQETTRQISELEKDLNSLTETKESLVSKLELRKKQFYLLINTIHELQRM 182

Query: 212 IEDEQRSLVEEMKTLSEEQKV 233
           I+DE+    +E   LS +Q +
Sbjct: 183 IDDEK---TDEGTALSPQQSI 194

[Close]

BLAST of Spo25312.1 vs. ExPASy Swiss-Prot
Match: THOC7_XENLA (THO complex subunit 7 homolog OS=Xenopus laevis GN=thoc7 PE=2 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.000e-11
Identity = 60/203 (29.56%), Postives = 102/203 (50.25%), Query Frame = 1


		  
Query: 28  GPHEDDAIIKHRLLTRTTTTRGEPPLKKLQKKFTALVVEVEKSTDNYNDCEKLAKAFLQE 87
           G   DD +I+ RLL        +  +  L K F        +  + Y+  +++    L  
Sbjct: 2   GAVTDDEVIRKRLLIDGDGAGDDRRINLLVKSFVKWCNSGSQE-EGYSQYQRM----LSS 61

Query: 88  LTTFEIPLLKSKAVVDANLREKEHFNELNDEINSQILEVQTEIEDLKKELEASKIERKHK 147
           L+  E  + K+  V D NLRE E++ ++  +I S I     +I + KK++  +K  RK++
Sbjct: 62  LSQCEYSMGKTLLVHDMNLREMENYEKIYVDIESSIAAAHEKIAECKKQILQAKRIRKNR 121

Query: 148 EECETIRKLIATQPPRSETEKMIAELEKEIAALEAEDMASARLLELRKKQFALLLHVVDE 207
           +E + + K+I   P R ET K +  L+KE+  L      +   LELR+KQF +LL  + E
Sbjct: 122 QEYDALAKVIEQHPDRHETLKQLEALDKELKQLSHTKENAEDKLELRRKQFHVLLSTIHE 181

Query: 208 LQVTIEDEQRSLVEEMKTLSEEQ 231
           LQ T+E++ +   E  ++  E Q
Sbjct: 182 LQQTLENDDKLAEESQESPMETQ 199

[Close]

BLAST of Spo25312.1 vs. ExPASy Swiss-Prot
Match: THOC7_XENTR (THO complex subunit 7 homolog OS=Xenopus tropicalis GN=thoc7 PE=2 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 2.300e-11
Identity = 60/203 (29.56%), Postives = 97/203 (47.78%), Query Frame = 1


		  
Query: 28  GPHEDDAIIKHRLLTRTTTTRGEPPLKKLQKKFTALVVEVEKSTDNYNDCEKLAKAFLQE 87
           G   DD +I+ RLL        +  +  L K F        +    Y       +  L  
Sbjct: 2   GAVTDDEVIRKRLLIDGDGAGDDRRINLLVKSFLKWCNSGSQEEGQY-------QRMLSS 61

Query: 88  LTTFEIPLLKSKAVVDANLREKEHFNELNDEINSQILEVQTEIEDLKKELEASKIERKHK 147
           L+  E  + K+  V D NLRE E++ ++  +I S I     +I + KK++  +K  RK++
Sbjct: 62  LSQCEFSMGKTLLVHDMNLREMENYEKIYVDIESSIAAAHEKIAECKKQILQAKRIRKNR 121

Query: 148 EECETIRKLIATQPPRSETEKMIAELEKEIAALEAEDMASARLLELRKKQFALLLHVVDE 207
           +E + + K+I   P R ET K +  L+KE+  L          LELR+KQF +LL  + E
Sbjct: 122 QEYDALAKVIQQHPDRHETLKQLEALDKELKQLSHTKENVEDKLELRRKQFHVLLSTIHE 181

Query: 208 LQVTIEDEQRSLVEEMKTLSEEQ 231
           LQ T+E++ +   E  ++  E Q
Sbjct: 182 LQQTLENDDKLAEESQESPMETQ 197

[Close]

BLAST of Spo25312.1 vs. TAIR (Arabidopsis)
Match: AT3G02950.1 (Tho complex subunit 7/Mft1p)

HSP 1 Score: 297.7 bits (761), Expect = 6.400e-81
Identity = 163/241 (67.63%), Postives = 199/241 (82.57%), Query Frame = 1


		  
Query: 9   LKGRRVVGKGEAVAA--HYAFGPHEDDAIIKHRLLTRTTTTRGEPPLKKLQKKFTALVVE 68
           +K RR+ G+ E V    +YAF P +DD II++RLLTRTTTTRGEPPLKKLQKKFT+ V+E
Sbjct: 3   VKARRISGRLETVVTKVNYAFDPVDDDKIIRNRLLTRTTTTRGEPPLKKLQKKFTSFVLE 62

Query: 69  VEKSTDNYNDCEKLAKAFLQELTTFEIPLLKSKAVVDANLREKEHFNELNDEINSQILEV 128
           V+K  +NYNDC +LAKAFLQEL+TFEIPLLKS+AVV+ANLREKE FNE+ DE   QI++ 
Sbjct: 63  VDKEEENYNDCGRLAKAFLQELSTFEIPLLKSQAVVEANLREKESFNEVKDETERQIMQA 122

Query: 129 QTEIEDLKKELEASKIERKHKEECETIRKLIATQPPRSETEKMIAELEKEIAALEAEDMA 188
           + EIEDLKK+LE SKI+R+HKEECETIRKLI+ QPPRSETEK+I EL KEIA LEAE  A
Sbjct: 123 KAEIEDLKKQLEESKIDRQHKEECETIRKLISAQPPRSETEKVIYELNKEIAELEAESTA 182

Query: 189 SARLLELRKKQFALLLHVVDELQVTIEDEQRSLVEEMKTLSEEQKVGPEDPSGSAEAMNI 248
           S RLLELRKKQFALL+HVVDELQ T+EDEQ+SLV+E+++ SE+Q+          +AM++
Sbjct: 183 SWRLLELRKKQFALLMHVVDELQNTMEDEQKSLVDEIRSASEDQR-------NITDAMSV 236

[Close]

BLAST of Spo25312.1 vs. TAIR (Arabidopsis)
Match: AT5G16790.1 (Tho complex subunit 7/Mft1p)

HSP 1 Score: 293.1 bits (749), Expect = 1.600e-79
Identity = 163/240 (67.92%), Postives = 196/240 (81.67%), Query Frame = 1


		  
Query: 9   LKGRRVV-GKGEAVAAHYAFGPHEDDAIIKHRLLTRTTTTRGEPPLKKLQKKFTALVVEV 68
           ++ RR+V G+ E VAA+YAF P  DD II++RLLTRTTTTRGEPPLKKLQKKFT+ V+E+
Sbjct: 3   VRARRIVSGRSETVAANYAFDPLHDDKIIRNRLLTRTTTTRGEPPLKKLQKKFTSFVIEL 62

Query: 69  EKSTDNYNDCEKLAKAFLQELTTFEIPLLKSKAVVDANLREKEHFNELNDEINSQILEVQ 128
           +K  DNY++C +LAKAFLQEL+ FEIPLLKS+ VV ANLREKE+FNEL DE N QI++ Q
Sbjct: 63  DKEEDNYSECGRLAKAFLQELSAFEIPLLKSQVVVAANLREKENFNELKDETNRQIMQAQ 122

Query: 129 TEIEDLKKELEASKIERKHKEECETIRKLIATQPPRSETEKMIAELEKEIAALEAEDMAS 188
            +IEDLKK+LE SKIER+ KEECE IRKLI+ QPPRSET+K+I EL+KEIA LEAE+ AS
Sbjct: 123 ADIEDLKKQLEESKIERQQKEECEAIRKLISAQPPRSETQKVIHELKKEIAELEAENTAS 182

Query: 189 ARLLELRKKQFALLLHVVDELQVTIEDEQRSLVEEMKTLSEEQKVGPEDPSGSAEAMNID 248
            RLLELRKKQFALLLHVVDELQ T+EDEQ+S+VEEM+             +  AEAM+ID
Sbjct: 183 WRLLELRKKQFALLLHVVDELQETMEDEQKSMVEEMQR---------NITADGAEAMSID 233

The following BLAST results are available for this feature:

BLAST of Spo25312.1 vs. NCBI nr
BLAST of Spo25312.1 vs. UniProtKB/TrEMBL
BLAST of Spo25312.1 vs. ExPASy Swiss-Prot
BLAST of Spo25312.1 vs. TAIR (Arabidopsis)

BLAST of Spo25312.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902163832\|gb\|KNA06890.1\|	4.1e-122	100.	hypothetical protein SOVF_1769...	[more]
gi\|731339190\|ref\|XP_010680719.1\|	1.5e-103	84.1	PREDICTED: THO complex subunit...	[more]
gi\|731339467\|ref\|XP_010680871.1\|	6.2e-102	81.6	PREDICTED: uncharacterized pro...	[more]
gi\|731339196\|ref\|XP_010680722.1\|	1.6e-97	85.7	PREDICTED: THO complex subunit...	[more]
gi\|661886750\|emb\|CDP09699.1\|	6.9e-93	76.6	unnamed protein product [Coffe...	[more]

BLAST of Spo25312.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9QI28_SPIOL	2.9e-122	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8F0B3_BETVU	1.0e-103	84.1	Uncharacterized protein OS=Bet...	[more]
A0A068UNB5_COFCA	4.8e-93	76.6	Uncharacterized protein OS=Cof...	[more]
A0A061F6H5_THECC	1.4e-92	76.8	Tho complex subunit 7/Mft1p OS...	[more]
A0A0B0PUA3_GOSAR	1.8e-92	76.8	THO complex subunit 7 OS=Gossy...	[more]

BLAST of Spo25312.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
THO7B_ARATH	1.1e-79	67.6	THO complex subunit 7B OS=Arab...	[more]
THO7A_ARATH	2.8e-78	67.9	THO complex subunit 7A OS=Arab...	[more]
THOC7_NEMVE	1.9e-13	32.8	THO complex subunit 7 homolog ...	[more]
THOC7_XENLA	1.0e-11	29.5	THO complex subunit 7 homolog ...	[more]
THOC7_XENTR	2.3e-11	29.5	THO complex subunit 7 homolog ...	[more]

BLAST of Spo25312.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 2


Match Name	E-value	Identity	Description
AT3G02950.1	6.4e-81	67.6	Tho complex subunit 7/Mft1p	[more]
AT5G16790.1	1.6e-79	67.9	Tho complex subunit 7/Mft1p	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008501	THO complex subunit 7/Mft1	PFAM	PF05615	THOC7	coord: 33..169 score: 8.5
None	No IPR available	unknown	Coil	Coil	coord: 116..150 score: -coord: 163..190 scor
None	No IPR available	PANTHER	PTHR23405	MAINTENANCE OF KILLER 16 MAK16 PROTEIN-RELATED	coord: 30..247 score: 8.7

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006397	mRNA processing
biological_process	GO:0000398	mRNA splicing, via spliceosome
biological_process	GO:0001510	RNA methylation
cellular_component	GO:0000445	THO complex part of transcription export complex
cellular_component	GO:0015030	Cajal body
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005730	nucleolus
cellular_component	GO:1990904	ribonucleoprotein complex
cellular_component	GO:0019013	viral nucleocapsid
molecular_function	GO:0005515	protein binding
molecular_function	GO:0003723	RNA binding

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo20239	0.67	Barchart \| Table
Spo21179	0.66	Barchart \| Table