Spo25621 (gene) | SpinachBase

Overview


Name	Spo25621
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Branched-chain-amino-acid aminotransferase-like protein 3
Location	chr1 : 37927110 .. 37930187 (+)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGATGTAGAATGTATAGTGATGCACCCTCTTGCATTATCCTCTACGGCTCTACAAATAATTGGTCATTGCTTCCATGGCTGCTCCTCTCTGCATTCCCCCACAGCACAGCTTACAAAACCAAAATAAATATGCAAAATCCTTTACTAAACATTATAATTTTCAATTTCTTGTTACAAGAAATCCCCCAAGTACTTTATTTGGGCTTCAATCGTCTAATCTTGGGGTTCCTAGACTCTCAAATCAAGCTCAGTCTTTAATTAGTAAATTCCTTCTTTTTTTTTTCTTTAATTTCCTTCCATTATCAGCTTTTAAAATTCTCGATTACTGTATTATAATCTATTTATTCTTGATATTCATTCAGTTTAACACTCATCTCTGATCATAATTTGGCTGTTTGGTTACTTGAAAACTTTAATTTTGACAGATTCAACCCCAATTTCGGAAGTTCCACTCTTTTCACGTGAAGAGGTTTGATACATTTTATCTGAATTGTTGTTTCAATATCATAAATTACGTTATTCATTTTTAGATAGTATCATCTGGTTTAAGTCTGTAATACGGAGATCATGAGGATCTATTTCTTTCGCCAATGTGTTCACATGCTAGGTACAACATGGGCAAAGTAATAGTCTCGTTTCATGTGATGCCAGAAGCAAGCCTAGTTCTTGTCAACAAAATGTATTATACACGTATTATTTAAATGAATGATCACTTTTCCAATGTGTTCAAAAATTGCAGGCAATTGAGAGGTTAAGGTTGAGTAAAGAGAGGCACAAGGGAAAACAACAATTTCTGGCTATGTACTGCAGTATATTTGGTGGGATAACAACAGACCCCGCGGCGATGGTGATCCCCATGGACGATCACATGGTACATAGAGGGCATGGTATATTTGACACCGCTGCTATTGTAGACGGGTATCATTTTGAGATGCTTCATAAAATTGAAATTATAGAAATATTTTTGTATTTTTTACAAATAATGGTGTGGCATAATTTGGACTCAGCCCCGGGTTTTGATTACTTCTTGTATGCAGTCATCTATACGAGTTGGATCAACACCTTGATCGTTGTGTGAGGTCTGCAACTATGGCGAAAATCAGACTACCATTCGATAGCGAAACCATGAGAAACATACTTATACAGACAGTAATTGCTTCCAAGTGTAAAAATGGATCTTTAAGATATTGGCTTTCGGCAGGATACGGCAACTTTCTTCTCTCTCCGTCTGGGTGTGCTGAGCCAACATTTTACGCCATTGTAATTCAAACCTCGGCCTCACAGCCCGATCACAAGGGTGTTAAAGTAATTACTTCTTCCATCCCAATAAAGCCTCCTGTGTTTGCTACAATGAAAAGTGTCAATTACCTTCCAAATGCACTATGCCAAATGGAAGCTGAAGAAAACGGTTGCTTCACATCTGTTTGGTTGGACACTGAAGGATTCGTTGCAGAGGGTCCGAATATGAACGTGGCCTTTGTCACGTATGATAAAGAGCTTTTGATGCCGAGGTTTGATAAAGTTCTTGGTGGATGTACACTCAAGAGAATGCGTACTCTAGCACAGGAACTGGTTAAGAAGGGCGACATTCGCGGAGTAAGCACGAGGGACGTGACCGTGGAAGAAGGGAAAAGGGCTGAGGAGATGATGCTTTTTGGAAGCGGCGTCCTAGTGCGTCCAGTTGTTCAATGGGATGACAAGATCATAGGCAATGGTAATAATTTTGCTTACATTTAAATGCTCTCTTTAGTTTATAGTAAAATGACGGAAGTTGAGATGCTAACAGCTTAAAAACCCAAGCTTTGAACATGTATACAACTCTGCATCTTAGCATTTGCACCTTCTTTACAATTACGACGAGTAGATTGTATTCGTGTCAGTACCTTATAGAGAACATAAGTCTTTTTGATATGGCCCCTCTGTCCCTAGTTAATAGAATTTAAGGAAATAAAGTACAACACATTAATCCATCAAAATTAAAACCAACCAAATGAGATTACAAAGCCTTTTACTAATAAACTAATAAAATTACAACGTTAAAAAGGAAAGAAAAGAAATGGAAAGATCGTTTTCAGACACCTTAAAAGGAAACGGTAAAAACAATTAGGCTCCGTTTGGCAGGGCGTAAAAGGTTTTCATGGAAAACGATTTTCCCCTTTTAAATCATTTCACGTTGTTTGGTTGGGTAAGGGATGGAAAACAATTTTCTATTACTCTCTCAATGATGGAAAACCCTTTTCCATTTGAAAGGGAGGGAAACCACTTTTCCTTCTTTCCTCCTTACCTCCTCTCCTTTCTTCCACTCAATCTTCACCATCTTCTCCCAGTTACCAAACAAATGAAAACTAGTTTGAAATTGTATTTTCCCTTGAAAACTGTTTTACATGAAAAATTGTTTTACAATGAAACGTTTTACGCCTTACCAAACAGAGCCTTAGGGCCGGAGGGAGTATATTTCTAAACATATGATTGTGTCTTCGTGGTGATGGATATTGTGCAGGAAATGAAGGTCCTATTGCTCGAGCACTTCTCGACCTCCTCCTAGAGGACATGATATCAGGCCCATCAACAGTTCGGACAGCCGTTCCTTACTAGGCACAATTCGCAAGAAGAGCATGGTTCTGTTATTTGTACATCAACGTACAGCACTTGGGATTATCAAAAGGCTGGAAAATCTTGTGCTATTTCTCCAGGTCCGATCCCCTAAAGGAAAAAGTAAATAAAAGAGCTCCACTGGTTGAAAATAAAAGGCCAAGGAAATGGATGGTGTACTGGTGGTTAATGCATCCAGTTATTCAGTGGATGAATAGGTCATTACGCCTCATTAGTCACAGGAAAAAAGAATTTCATATCCCCTTTTTTTTTTTCAAGTCCTGTAAAAACTACTTCCTGCTCTGGTTTATAATTTACCACGAGAACATGCAGCTTTGTTATTTTTACCTCCAATAAAAAAGATACGGGAATAATAAGGTTAGACAAATTCAAAGAAGGGCTAGTCATAACAGGTGCAGTAATTATAACTCCAAGCATAGCTGTATAAGTTCGGTTAAAGGTTTACAGTCCAGTACAATTCCCTCC

mRNA sequence

TGATGTAGAATGTATAGTGATGCACCCTCTTGCATTATCCTCTACGGCTCTACAAATAATTGGTCATTGCTTCCATGGCTGCTCCTCTCTGCATTCCCCCACAGCACAGCTTACAAAACCAAAATAAATATGCAAAATCCTTTACTAAACATTATAATTTTCAATTTCTTGTTACAAGAAATCCCCCAAGTACTTTATTTGGGCTTCAATCGTCTAATCTTGGGGTTCCTAGACTCTCAAATCAAGCTCAGTCTTTAATTAATTCAACCCCAATTTCGGAAGTTCCACTCTTTTCACGTGAAGAGGCAATTGAGAGGTTAAGGTTGAGTAAAGAGAGGCACAAGGGAAAACAACAATTTCTGGCTATGTACTGCAGTATATTTGGTGGGATAACAACAGACCCCGCGGCGATGGTGATCCCCATGGACGATCACATGGTACATAGAGGGCATGGTATATTTGACACCGCTGCTATTGTAGACGGTCATCTATACGAGTTGGATCAACACCTTGATCGTTGTGTGAGGTCTGCAACTATGGCGAAAATCAGACTACCATTCGATAGCGAAACCATGAGAAACATACTTATACAGACAGTAATTGCTTCCAAGTGTAAAAATGGATCTTTAAGATATTGGCTTTCGGCAGGATACGGCAACTTTCTTCTCTCTCCGTCTGGGTGTGCTGAGCCAACATTTTACGCCATTGTAATTCAAACCTCGGCCTCACAGCCCGATCACAAGGGTGTTAAAGTAATTACTTCTTCCATCCCAATAAAGCCTCCTGTGTTTGCTACAATGAAAAGTGTCAATTACCTTCCAAATGCACTATGCCAAATGGAAGCTGAAGAAAACGGTTGCTTCACATCTGTTTGGTTGGACACTGAAGGATTCGTTGCAGAGGGTCCGAATATGAACGTGGCCTTTGTCACGTATGATAAAGAGCTTTTGATGCCGAGGTTTGATAAAGTTCTTGGTGGATGTACACTCAAGAGAATGCGTACTCTAGCACAGGAACTGGTTAAGAAGGGCGACATTCGCGGAGTAAGCACGAGGGACGTGACCGTGGAAGAAGGGAAAAGGGCTGAGGAGATGATGCTTTTTGGAAGCGGCGTCCTAGTGCGTCCAGTTGTTCAATGGGATGACAAGATCATAGGCAATGGAAATGAAGGTCCTATTGCTCGAGCACTTCTCGACCTCCTCCTAGAGGACATGATATCAGGCCCATCAACAGTTCGGACAGCCGTTCCTTACTAGGCACAATTCGCAAGAAGAGCATGGTTCTGTTATTTGTACATCAACGTACAGCACTTGGGATTATCAAAAGGCTGGAAAATCTTGTGCTATTTCTCCAGGTCCGATCCCCTAAAGGAAAAAGTAAATAAAAGAGCTCCACTGGTTGAAAATAAAAGGCCAAGGAAATGGATGGTGTACTGGTGGTTAATGCATCCAGTTATTCAGTGGATGAATAGGTCATTACGCCTCATTAGTCACAGGAAAAAAGAATTTCATATCCCCTTTTTTTTTTTCAAGTCCTGTAAAAACTACTTCCTGCTCTGGTTTATAATTTACCACGAGAACATGCAGCTTTGTTATTTTTACCTCCAATAAAAAAGATACGGGAATAATAAGGTTAGACAAATTCAAAGAAGGGCTAGTCATAACAGGTGCAGTAATTATAACTCCAAGCATAGCTGTATAAGTTCGGTTAAAGGTTTACAGTCCAGTACAATTCCCTCC

Coding sequence (CDS)

ATGGCTGCTCCTCTCTGCATTCCCCCACAGCACAGCTTACAAAACCAAAATAAATATGCAAAATCCTTTACTAAACATTATAATTTTCAATTTCTTGTTACAAGAAATCCCCCAAGTACTTTATTTGGGCTTCAATCGTCTAATCTTGGGGTTCCTAGACTCTCAAATCAAGCTCAGTCTTTAATTAATTCAACCCCAATTTCGGAAGTTCCACTCTTTTCACGTGAAGAGGCAATTGAGAGGTTAAGGTTGAGTAAAGAGAGGCACAAGGGAAAACAACAATTTCTGGCTATGTACTGCAGTATATTTGGTGGGATAACAACAGACCCCGCGGCGATGGTGATCCCCATGGACGATCACATGGTACATAGAGGGCATGGTATATTTGACACCGCTGCTATTGTAGACGGTCATCTATACGAGTTGGATCAACACCTTGATCGTTGTGTGAGGTCTGCAACTATGGCGAAAATCAGACTACCATTCGATAGCGAAACCATGAGAAACATACTTATACAGACAGTAATTGCTTCCAAGTGTAAAAATGGATCTTTAAGATATTGGCTTTCGGCAGGATACGGCAACTTTCTTCTCTCTCCGTCTGGGTGTGCTGAGCCAACATTTTACGCCATTGTAATTCAAACCTCGGCCTCACAGCCCGATCACAAGGGTGTTAAAGTAATTACTTCTTCCATCCCAATAAAGCCTCCTGTGTTTGCTACAATGAAAAGTGTCAATTACCTTCCAAATGCACTATGCCAAATGGAAGCTGAAGAAAACGGTTGCTTCACATCTGTTTGGTTGGACACTGAAGGATTCGTTGCAGAGGGTCCGAATATGAACGTGGCCTTTGTCACGTATGATAAAGAGCTTTTGATGCCGAGGTTTGATAAAGTTCTTGGTGGATGTACACTCAAGAGAATGCGTACTCTAGCACAGGAACTGGTTAAGAAGGGCGACATTCGCGGAGTAAGCACGAGGGACGTGACCGTGGAAGAAGGGAAAAGGGCTGAGGAGATGATGCTTTTTGGAAGCGGCGTCCTAGTGCGTCCAGTTGTTCAATGGGATGACAAGATCATAGGCAATGGAAATGAAGGTCCTATTGCTCGAGCACTTCTCGACCTCCTCCTAGAGGACATGATATCAGGCCCATCAACAGTTCGGACAGCCGTTCCTTACTAG

Protein sequence

MAAPLCIPPQHSLQNQNKYAKSFTKHYNFQFLVTRNPPSTLFGLQSSNLGVPRLSNQAQSLINSTPISEVPLFSREEAIERLRLSKERHKGKQQFLAMYCSIFGGITTDPAAMVIPMDDHMVHRGHGIFDTAAIVDGHLYELDQHLDRCVRSATMAKIRLPFDSETMRNILIQTVIASKCKNGSLRYWLSAGYGNFLLSPSGCAEPTFYAIVIQTSASQPDHKGVKVITSSIPIKPPVFATMKSVNYLPNALCQMEAEENGCFTSVWLDTEGFVAEGPNMNVAFVTYDKELLMPRFDKVLGGCTLKRMRTLAQELVKKGDIRGVSTRDVTVEEGKRAEEMMLFGSGVLVRPVVQWDDKIIGNGNEGPIARALLDLLLEDMISGPSTVRTAVPY

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo25621.1	Spo25621.1	mRNA

Homology

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BLAST of Spo25621.1 vs. NCBI nr
Match: gi|870867103|gb|KMT18002.1| (hypothetical protein BVRB_2g031960 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 581.6 bits (1498), Expect = 1.000e-162
Identity = 294/393 (74.81%), Postives = 327/393 (83.21%), Query Frame = 1


		  
Query: 3   APLCIPPQHSLQNQNKYAKSFTKHYNFQFLVTRNPPSTLFGLQSSNLGVPRLSNQAQS-- 62
           A L    QH LQ Q     SF    N     +R+     FG+  S LG+ R SN A +  
Sbjct: 2   ASLSNSSQHILQTQQISTNSFPNLNNSLCATSRDVSFLRFGIPRSRLGLLRFSNHADAHP 61

Query: 63  LINSTPISEVPLFSREEAIERLRLSKERHKGKQQFLAMYCSIFGGITTDPAAMVIPMDDH 122
            I++T I E+PL S EEAIERLRL + RHKGKQQ+LAMY SIFGGITTDPAAMVIPMDDH
Sbjct: 62  SIDTTQIFELPLLSSEEAIERLRLCRVRHKGKQQYLAMYSSIFGGITTDPAAMVIPMDDH 121

Query: 123 MVHRGHGIFDTAAIVDGHLYELDQHLDRCVRSATMAKIRLPFDSETMRNILIQTVIASKC 182
           MVHRGHG+FDTAAIVDG+LYELDQHLDR V+SA+MAKI  PFD ETMR+IL+QTV AS+C
Sbjct: 122 MVHRGHGVFDTAAIVDGNLYELDQHLDRFVQSASMAKINQPFDRETMRSILLQTVSASQC 181

Query: 183 KNGSLRYWLSAGYGNFLLSPSGCAEPTFYAIVIQTSASQPDHKGVKVITSSIPIKPPVFA 242
           KNGSLRYWLSAGYG+FLLSPSGC E TFYAIV Q+S S  D KG+KVITSSIPIKPPVFA
Sbjct: 182 KNGSLRYWLSAGYGDFLLSPSGCVESTFYAIVYQSSNSY-DPKGIKVITSSIPIKPPVFA 241

Query: 243 TMKSVNYLPNALCQMEAEENGCFTSVWLDTEGFVAEGPNMNVAFVTYDKELLMPRFDKVL 302
           TMKSVNYLPNALCQMEAEENGCF S+WLD EGF+AEGPNMNVAFVTY+KELL+P+FD +L
Sbjct: 242 TMKSVNYLPNALCQMEAEENGCFASIWLDLEGFIAEGPNMNVAFVTYEKELLIPKFDNIL 301

Query: 303 GGCTLKRMRTLAQELVKKGDIRGVSTRDVTVEEGKRAEEMMLFGSGVLVRPVVQWDDKII 362
            GCT KR  +L+QELVKKG+IRGVS RDVTVEEGKRAEEMMLFGSGVLVRP++QWDD++I
Sbjct: 302 NGCTAKRALSLSQELVKKGEIRGVSVRDVTVEEGKRAEEMMLFGSGVLVRPIIQWDDQVI 361

Query: 363 GNGNEGPIARALLDLLLEDMISGPSTVRTAVPY 394
           GNG EGPIARALLDLLLEDM SGPSTVRT VPY
Sbjct: 362 GNGKEGPIARALLDLLLEDMKSGPSTVRTPVPY 393

[Close]

BLAST of Spo25621.1 vs. NCBI nr
Match: gi|731317190|ref|XP_010668094.1| (PREDICTED: D-amino-acid transaminase, chloroplastic [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 575.5 bits (1482), Expect = 7.200e-161
Identity = 294/398 (73.87%), Postives = 327/398 (82.16%), Query Frame = 1


		  
Query: 3   APLCIPPQHSLQNQNKYAKSFTKHYNFQFLVTRNPPSTLFGLQSSNLGVPRLSNQAQS-- 62
           A L    QH LQ Q     SF    N     +R+     FG+  S LG+ R SN A +  
Sbjct: 2   ASLSNSSQHILQTQQISTNSFPNLNNSLCATSRDVSFLRFGIPRSRLGLLRFSNHADAHP 61

Query: 63  LINSTPISEVPLFSREE-----AIERLRLSKERHKGKQQFLAMYCSIFGGITTDPAAMVI 122
            I++T I E+PL S EE     AIERLRL + RHKGKQQ+LAMY SIFGGITTDPAAMVI
Sbjct: 62  SIDTTQIFELPLLSSEEVETFQAIERLRLCRVRHKGKQQYLAMYSSIFGGITTDPAAMVI 121

Query: 123 PMDDHMVHRGHGIFDTAAIVDGHLYELDQHLDRCVRSATMAKIRLPFDSETMRNILIQTV 182
           PMDDHMVHRGHG+FDTAAIVDG+LYELDQHLDR V+SA+MAKI  PFD ETMR+IL+QTV
Sbjct: 122 PMDDHMVHRGHGVFDTAAIVDGNLYELDQHLDRFVQSASMAKINQPFDRETMRSILLQTV 181

Query: 183 IASKCKNGSLRYWLSAGYGNFLLSPSGCAEPTFYAIVIQTSASQPDHKGVKVITSSIPIK 242
            AS+CKNGSLRYWLSAGYG+FLLSPSGC E TFYAIV Q+S S  D KG+KVITSSIPIK
Sbjct: 182 SASQCKNGSLRYWLSAGYGDFLLSPSGCVESTFYAIVYQSSNSY-DPKGIKVITSSIPIK 241

Query: 243 PPVFATMKSVNYLPNALCQMEAEENGCFTSVWLDTEGFVAEGPNMNVAFVTYDKELLMPR 302
           PPVFATMKSVNYLPNALCQMEAEENGCF S+WLD EGF+AEGPNMNVAFVTY+KELL+P+
Sbjct: 242 PPVFATMKSVNYLPNALCQMEAEENGCFASIWLDLEGFIAEGPNMNVAFVTYEKELLIPK 301

Query: 303 FDKVLGGCTLKRMRTLAQELVKKGDIRGVSTRDVTVEEGKRAEEMMLFGSGVLVRPVVQW 362
           FD +L GCT KR  +L+QELVKKG+IRGVS RDVTVEEGKRAEEMMLFGSGVLVRP++QW
Sbjct: 302 FDNILNGCTAKRALSLSQELVKKGEIRGVSVRDVTVEEGKRAEEMMLFGSGVLVRPIIQW 361

Query: 363 DDKIIGNGNEGPIARALLDLLLEDMISGPSTVRTAVPY 394
           DD++IGNG EGPIARALLDLLLEDM SGPSTVRT VPY
Sbjct: 362 DDQVIGNGKEGPIARALLDLLLEDMKSGPSTVRTPVPY 398

[Close]

BLAST of Spo25621.1 vs. NCBI nr
Match: gi|902140766|gb|KNA04650.1| (hypothetical protein SOVF_197660 [Spinacia oleracea])

HSP 1 Score: 512.3 bits (1318), Expect = 7.500e-142
Identity = 260/296 (87.84%), Postives = 262/296 (88.51%), Query Frame = 1


		  
Query: 98  MYCSIFGGITTDPAAMVIPMDDHMVHRGHGIFDTAAIVDGHLYELDQHLDRCVRSATMAK 157
           MYCSIFGGITTDPAAMVIPMDDHMVHRGHGIFDTAAIVDGHLYELDQHLDRCVRSATMAK
Sbjct: 1   MYCSIFGGITTDPAAMVIPMDDHMVHRGHGIFDTAAIVDGHLYELDQHLDRCVRSATMAK 60

Query: 158 IRLPFDSETMRNILIQTVIASKCKNGSLRYWLSAGYGNFLLSPSGCAEPTFYAIVIQTSA 217
           IRLPFD ETMRNILIQTVIASKCKNGSLRYWLSAGYGNFLLSPSGCAEPTFYAIVIQTSA
Sbjct: 61  IRLPFDRETMRNILIQTVIASKCKNGSLRYWLSAGYGNFLLSPSGCAEPTFYAIVIQTSA 120

Query: 218 SQPDHKGVKVITSSIPIKPPVFATMKSVNYLPNALCQMEAEENGCFTSVWLDTEGFVAEG 277
           SQPDHKGVK+               K+V                  TSVWLDTEGFVAEG
Sbjct: 121 SQPDHKGVKL--------------KKTVA-----------------TSVWLDTEGFVAEG 180

Query: 278 PNMNVAFVTYDKELLMPRFDKVLGGCTLKRMRTLAQELVKKGDIRGVSTRDVTVEEGKRA 337
           PNMNVAFVTYDKELLMPRFDKVLGGCTLKRMRTLAQELVKKGDIRGVSTRDVTVEEGKRA
Sbjct: 181 PNMNVAFVTYDKELLMPRFDKVLGGCTLKRMRTLAQELVKKGDIRGVSTRDVTVEEGKRA 240

Query: 338 EEMMLFGSGVLVRPVVQWDDKIIGNGNEGPIARALLDLLLEDMISGPSTVRTAVPY 394
           EEMMLFGSGVLVRPVVQWDDKIIGNGNEGPIARALLDLLLEDMISGPSTVRTAVPY
Sbjct: 241 EEMMLFGSGVLVRPVVQWDDKIIGNGNEGPIARALLDLLLEDMISGPSTVRTAVPY 265

[Close]

BLAST of Spo25621.1 vs. NCBI nr
Match: gi|743842006|ref|XP_011026599.1| (PREDICTED: D-amino-acid transaminase, chloroplastic-like isoform X1 [Populus euphratica])

HSP 1 Score: 494.2 bits (1271), Expect = 2.100e-136
Identity = 250/390 (64.10%), Postives = 305/390 (78.21%), Query Frame = 1


		  
Query: 9   PQHSLQNQNKYAKSFTKHYNFQFLVTRNPPSTLFGLQSSN-----LGVPRLSNQAQSLIN 68
           P+ S Q+ N  + +   H +   LV RN      GL S +     + + R S+QA++L++
Sbjct: 9   PKPSFQHPNT-SINLPDHLHNSCLVPRNLSFQRLGLISQHGLFGKMKIARCSHQAEALVD 68

Query: 69  S-TPISEVPLFSREEAIERLRLSKERHKGKQQFLAMYCSIFGGITTDPAAMVIPMDDHMV 128
           S T IS+VP+ +  EA ERL+ ++E  KGKQQFLAMY SIFGGITTD +AMVIP+DDHMV
Sbjct: 69  SNTQISDVPILTCSEAFERLKKNRENQKGKQQFLAMYSSIFGGITTDTSAMVIPLDDHMV 128

Query: 129 HRGHGIFDTAAIVDGHLYELDQHLDRCVRSATMAKIRLPFDSETMRNILIQTVIASKCKN 188
           HRGHG+FDTAAIVDGHLYE DQHLDR +RSA++AKI LPFD E +R ILIQTV ASKCK 
Sbjct: 129 HRGHGVFDTAAIVDGHLYEFDQHLDRILRSASLAKINLPFDRENIRRILIQTVSASKCKT 188

Query: 189 GSLRYWLSAGYGNFLLSPSGCAEPTFYAIVIQTSASQPDHKGVKVITSSIPIKPPVFATM 248
           GSLRYWLSAG G+F LSPS C +P  YAIVIQ  + + D +G+KV+TSS+PIKPP FAT+
Sbjct: 189 GSLRYWLSAGPGDFQLSPSDCHQPALYAIVIQDQSPR-DSRGIKVVTSSVPIKPPQFATV 248

Query: 249 KSVNYLPNALCQMEAEENGCFTSVWLDTEGFVAEGPNMNVAFVTYDKELLMPRFDKVLGG 308
           KSVNYLPNAL +MEAEENG + S+WLD +GFVAEGP+MNVAFVT +K+LLMP FDK+L G
Sbjct: 249 KSVNYLPNALSKMEAEENGAYASIWLDNDGFVAEGPSMNVAFVTKEKDLLMPAFDKILSG 308

Query: 309 CTLKRMRTLAQELVKKGDIRGVSTRDVTVEEGKRAEEMMLFGSGVLVRPVVQWDDKIIGN 368
           CT KR+ TLA+ LVK+G + G+   DVTVEEGK+A+EMML GSGVLVRPVVQWD+++IG+
Sbjct: 309 CTAKRVLTLAEGLVKEGKLHGIKIDDVTVEEGKKADEMMLIGSGVLVRPVVQWDNQVIGD 368

Query: 369 GNEGPIARALLDLLLEDMISGPSTVRTAVP 393
           G EGPI RALL L+LEDM SGP  VR  VP
Sbjct: 369 GKEGPITRALLALILEDMKSGPPAVRVPVP 396

[Close]

BLAST of Spo25621.1 vs. NCBI nr
Match: gi|566213507|ref|XP_002324812.2| (hypothetical protein POPTR_0018s00630g [Populus trichocarpa])

HSP 1 Score: 489.2 bits (1258), Expect = 6.800e-135
Identity = 249/390 (63.85%), Postives = 301/390 (77.18%), Query Frame = 1


		  
Query: 9   PQHSLQNQNKYAKSFTKHYNFQFLVTRNPPSTLFGLQSSN-----LGVPRLSNQAQSLIN 68
           P+ S Q+ N  + +   H +   LV RN      GL S       + + R S+QA++L++
Sbjct: 9   PKPSFQHPNT-SINLPDHLHNSCLVPRNLSFQRLGLISQQGLFGKVKIARCSHQAEALVD 68

Query: 69  S-TPISEVPLFSREEAIERLRLSKERHKGKQQFLAMYCSIFGGITTDPAAMVIPMDDHMV 128
           S T IS+VP+ +  EA ERL+ ++E  KGKQQFLAMY SIFGGITTD +AMVIP+DDHMV
Sbjct: 69  SNTQISDVPILTCSEAFERLKKNRENQKGKQQFLAMYSSIFGGITTDTSAMVIPLDDHMV 128

Query: 129 HRGHGIFDTAAIVDGHLYELDQHLDRCVRSATMAKIRLPFDSETMRNILIQTVIASKCKN 188
           HRGHG+FDTAAIVDGHLYE DQHLDR +RSA++AKI LPFD E +R ILIQTV ASKCK 
Sbjct: 129 HRGHGVFDTAAIVDGHLYEFDQHLDRILRSASLAKINLPFDRENIRRILIQTVSASKCKT 188

Query: 189 GSLRYWLSAGYGNFLLSPSGCAEPTFYAIVIQTSASQPDHKGVKVITSSIPIKPPVFATM 248
           GSLRYWLSAG G+F LSPS C +P  YAIVIQ   S  D +G+KV+TSS+PIKPP FAT+
Sbjct: 189 GSLRYWLSAGPGDFQLSPSDCHQPALYAIVIQDK-SPHDSRGIKVVTSSVPIKPPQFATV 248

Query: 249 KSVNYLPNALCQMEAEENGCFTSVWLDTEGFVAEGPNMNVAFVTYDKELLMPRFDKVLGG 308
           KSVNYLPNAL +MEAEEN  + S+WLD +GFVAEGP+MNVAFVT +K+LLMP FDK+L G
Sbjct: 249 KSVNYLPNALSKMEAEENDAYASIWLDNDGFVAEGPSMNVAFVTKEKDLLMPAFDKILSG 308

Query: 309 CTLKRMRTLAQELVKKGDIRGVSTRDVTVEEGKRAEEMMLFGSGVLVRPVVQWDDKIIGN 368
           CT KR+ TLA+ LVK+G + G+   DVTVEEGK+A+EMML GSGVLVRP VQWD+++IG+
Sbjct: 309 CTAKRVLTLAEGLVKEGKLHGIKIDDVTVEEGKKADEMMLIGSGVLVRPAVQWDNQVIGD 368

Query: 369 GNEGPIARALLDLLLEDMISGPSTVRTAVP 393
           G EGPI RALL L+LEDM SGP  VR  VP
Sbjct: 369 GKEGPITRALLALILEDMKSGPPAVRVPVP 396

[Close]

BLAST of Spo25621.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CWP4_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g031960 PE=3 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 7.000e-163
Identity = 294/393 (74.81%), Postives = 327/393 (83.21%), Query Frame = 1


		  
Query: 3   APLCIPPQHSLQNQNKYAKSFTKHYNFQFLVTRNPPSTLFGLQSSNLGVPRLSNQAQS-- 62
           A L    QH LQ Q     SF    N     +R+     FG+  S LG+ R SN A +  
Sbjct: 2   ASLSNSSQHILQTQQISTNSFPNLNNSLCATSRDVSFLRFGIPRSRLGLLRFSNHADAHP 61

Query: 63  LINSTPISEVPLFSREEAIERLRLSKERHKGKQQFLAMYCSIFGGITTDPAAMVIPMDDH 122
            I++T I E+PL S EEAIERLRL + RHKGKQQ+LAMY SIFGGITTDPAAMVIPMDDH
Sbjct: 62  SIDTTQIFELPLLSSEEAIERLRLCRVRHKGKQQYLAMYSSIFGGITTDPAAMVIPMDDH 121

Query: 123 MVHRGHGIFDTAAIVDGHLYELDQHLDRCVRSATMAKIRLPFDSETMRNILIQTVIASKC 182
           MVHRGHG+FDTAAIVDG+LYELDQHLDR V+SA+MAKI  PFD ETMR+IL+QTV AS+C
Sbjct: 122 MVHRGHGVFDTAAIVDGNLYELDQHLDRFVQSASMAKINQPFDRETMRSILLQTVSASQC 181

Query: 183 KNGSLRYWLSAGYGNFLLSPSGCAEPTFYAIVIQTSASQPDHKGVKVITSSIPIKPPVFA 242
           KNGSLRYWLSAGYG+FLLSPSGC E TFYAIV Q+S S  D KG+KVITSSIPIKPPVFA
Sbjct: 182 KNGSLRYWLSAGYGDFLLSPSGCVESTFYAIVYQSSNSY-DPKGIKVITSSIPIKPPVFA 241

Query: 243 TMKSVNYLPNALCQMEAEENGCFTSVWLDTEGFVAEGPNMNVAFVTYDKELLMPRFDKVL 302
           TMKSVNYLPNALCQMEAEENGCF S+WLD EGF+AEGPNMNVAFVTY+KELL+P+FD +L
Sbjct: 242 TMKSVNYLPNALCQMEAEENGCFASIWLDLEGFIAEGPNMNVAFVTYEKELLIPKFDNIL 301

Query: 303 GGCTLKRMRTLAQELVKKGDIRGVSTRDVTVEEGKRAEEMMLFGSGVLVRPVVQWDDKII 362
            GCT KR  +L+QELVKKG+IRGVS RDVTVEEGKRAEEMMLFGSGVLVRP++QWDD++I
Sbjct: 302 NGCTAKRALSLSQELVKKGEIRGVSVRDVTVEEGKRAEEMMLFGSGVLVRPIIQWDDQVI 361

Query: 363 GNGNEGPIARALLDLLLEDMISGPSTVRTAVPY 394
           GNG EGPIARALLDLLLEDM SGPSTVRT VPY
Sbjct: 362 GNGKEGPIARALLDLLLEDMKSGPSTVRTPVPY 393

[Close]

BLAST of Spo25621.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QBM5_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_197660 PE=3 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 5.200e-142
Identity = 260/296 (87.84%), Postives = 262/296 (88.51%), Query Frame = 1


		  
Query: 98  MYCSIFGGITTDPAAMVIPMDDHMVHRGHGIFDTAAIVDGHLYELDQHLDRCVRSATMAK 157
           MYCSIFGGITTDPAAMVIPMDDHMVHRGHGIFDTAAIVDGHLYELDQHLDRCVRSATMAK
Sbjct: 1   MYCSIFGGITTDPAAMVIPMDDHMVHRGHGIFDTAAIVDGHLYELDQHLDRCVRSATMAK 60

Query: 158 IRLPFDSETMRNILIQTVIASKCKNGSLRYWLSAGYGNFLLSPSGCAEPTFYAIVIQTSA 217
           IRLPFD ETMRNILIQTVIASKCKNGSLRYWLSAGYGNFLLSPSGCAEPTFYAIVIQTSA
Sbjct: 61  IRLPFDRETMRNILIQTVIASKCKNGSLRYWLSAGYGNFLLSPSGCAEPTFYAIVIQTSA 120

Query: 218 SQPDHKGVKVITSSIPIKPPVFATMKSVNYLPNALCQMEAEENGCFTSVWLDTEGFVAEG 277
           SQPDHKGVK+               K+V                  TSVWLDTEGFVAEG
Sbjct: 121 SQPDHKGVKL--------------KKTVA-----------------TSVWLDTEGFVAEG 180

Query: 278 PNMNVAFVTYDKELLMPRFDKVLGGCTLKRMRTLAQELVKKGDIRGVSTRDVTVEEGKRA 337
           PNMNVAFVTYDKELLMPRFDKVLGGCTLKRMRTLAQELVKKGDIRGVSTRDVTVEEGKRA
Sbjct: 181 PNMNVAFVTYDKELLMPRFDKVLGGCTLKRMRTLAQELVKKGDIRGVSTRDVTVEEGKRA 240

Query: 338 EEMMLFGSGVLVRPVVQWDDKIIGNGNEGPIARALLDLLLEDMISGPSTVRTAVPY 394
           EEMMLFGSGVLVRPVVQWDDKIIGNGNEGPIARALLDLLLEDMISGPSTVRTAVPY
Sbjct: 241 EEMMLFGSGVLVRPVVQWDDKIIGNGNEGPIARALLDLLLEDMISGPSTVRTAVPY 265

[Close]

BLAST of Spo25621.1 vs. UniProtKB/TrEMBL
Match: B9IKL7_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0018s00630g PE=3 SV=2)

HSP 1 Score: 489.2 bits (1258), Expect = 4.700e-135
Identity = 249/390 (63.85%), Postives = 301/390 (77.18%), Query Frame = 1


		  
Query: 9   PQHSLQNQNKYAKSFTKHYNFQFLVTRNPPSTLFGLQSSN-----LGVPRLSNQAQSLIN 68
           P+ S Q+ N  + +   H +   LV RN      GL S       + + R S+QA++L++
Sbjct: 9   PKPSFQHPNT-SINLPDHLHNSCLVPRNLSFQRLGLISQQGLFGKVKIARCSHQAEALVD 68

Query: 69  S-TPISEVPLFSREEAIERLRLSKERHKGKQQFLAMYCSIFGGITTDPAAMVIPMDDHMV 128
           S T IS+VP+ +  EA ERL+ ++E  KGKQQFLAMY SIFGGITTD +AMVIP+DDHMV
Sbjct: 69  SNTQISDVPILTCSEAFERLKKNRENQKGKQQFLAMYSSIFGGITTDTSAMVIPLDDHMV 128

Query: 129 HRGHGIFDTAAIVDGHLYELDQHLDRCVRSATMAKIRLPFDSETMRNILIQTVIASKCKN 188
           HRGHG+FDTAAIVDGHLYE DQHLDR +RSA++AKI LPFD E +R ILIQTV ASKCK 
Sbjct: 129 HRGHGVFDTAAIVDGHLYEFDQHLDRILRSASLAKINLPFDRENIRRILIQTVSASKCKT 188

Query: 189 GSLRYWLSAGYGNFLLSPSGCAEPTFYAIVIQTSASQPDHKGVKVITSSIPIKPPVFATM 248
           GSLRYWLSAG G+F LSPS C +P  YAIVIQ   S  D +G+KV+TSS+PIKPP FAT+
Sbjct: 189 GSLRYWLSAGPGDFQLSPSDCHQPALYAIVIQDK-SPHDSRGIKVVTSSVPIKPPQFATV 248

Query: 249 KSVNYLPNALCQMEAEENGCFTSVWLDTEGFVAEGPNMNVAFVTYDKELLMPRFDKVLGG 308
           KSVNYLPNAL +MEAEEN  + S+WLD +GFVAEGP+MNVAFVT +K+LLMP FDK+L G
Sbjct: 249 KSVNYLPNALSKMEAEENDAYASIWLDNDGFVAEGPSMNVAFVTKEKDLLMPAFDKILSG 308

Query: 309 CTLKRMRTLAQELVKKGDIRGVSTRDVTVEEGKRAEEMMLFGSGVLVRPVVQWDDKIIGN 368
           CT KR+ TLA+ LVK+G + G+   DVTVEEGK+A+EMML GSGVLVRP VQWD+++IG+
Sbjct: 309 CTAKRVLTLAEGLVKEGKLHGIKIDDVTVEEGKKADEMMLIGSGVLVRPAVQWDNQVIGD 368

Query: 369 GNEGPIARALLDLLLEDMISGPSTVRTAVP 393
           G EGPI RALL L+LEDM SGP  VR  VP
Sbjct: 369 GKEGPITRALLALILEDMKSGPPAVRVPVP 396

[Close]

BLAST of Spo25621.1 vs. UniProtKB/TrEMBL
Match: V4WBW3_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10008593mg PE=3 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 1.500e-133
Identity = 239/342 (69.88%), Postives = 283/342 (82.75%), Query Frame = 1


		  
Query: 53  RLSNQAQSLINSTP-ISEVPLFSREEAIERLRLSKERHKGKQQFLAMYCSIFGGITTDPA 112
           R   + ++LI+S+  +S+VPL S  EAIER++ ++   K KQQFLAMY SIFGGITTDPA
Sbjct: 47  RSCGRTEALIDSSAQLSDVPLLSCSEAIERIKSTQANQKSKQQFLAMYSSIFGGITTDPA 106

Query: 113 AMVIPMDDHMVHRGHGIFDTAAIVDGHLYELDQHLDRCVRSATMAKIRLPFDSETMRNIL 172
           AMVIPMDDHMVHRGHG+FDTAAI DG+LYELDQHLDR +RSA+MAKI+LPFD E++R IL
Sbjct: 107 AMVIPMDDHMVHRGHGVFDTAAICDGYLYELDQHLDRIIRSASMAKIQLPFDRESLRRIL 166

Query: 173 IQTVIASKCKNGSLRYWLSAGYGNFLLSPSGCAEPTFYAIVIQTSASQPDHKGVKVITSS 232
           IQTV ASKC+ GSLRYWLSAG G+F LSP GC + TFY I IQ   S    KGVKVITSS
Sbjct: 167 IQTVSASKCRKGSLRYWLSAGVGDFQLSPVGCHQSTFYVIEIQDD-SPFVSKGVKVITSS 226

Query: 233 IPIKPPVFATMKSVNYLPNALCQMEAEENGCFTSVWLDTEGFVAEGPNMNVAFVTYDKEL 292
           IPIKPP F T+KSVNYLPN L +MEAEE G F ++WLD EGF+AEGPNMNVAFVT ++ L
Sbjct: 227 IPIKPPQFGTVKSVNYLPNVLSKMEAEETGAFAAIWLDGEGFIAEGPNMNVAFVTKERLL 286

Query: 293 LMPRFDKVLGGCTLKRMRTLAQELVKKGDIRGVSTRDVTVEEGKRAEEMMLFGSGVLVRP 352
           LMP+FDK+L GCT KR+ TLA+ LV++G + G+   +VTVEEGK+AEEMML GSGVLVRP
Sbjct: 287 LMPQFDKILSGCTAKRVLTLAKALVREGKLHGIKVGNVTVEEGKKAEEMMLLGSGVLVRP 346

Query: 353 VVQWDDKIIGNGNEGPIARALLDLLLEDMISGPSTVRTAVPY 394
           VVQWD+++IGNG EGPIA+ALLDL+LEDM SGP +VR AVPY
Sbjct: 347 VVQWDEQVIGNGKEGPIAQALLDLILEDMQSGPPSVRVAVPY 387

[Close]

BLAST of Spo25621.1 vs. UniProtKB/TrEMBL
Match: A0A0D2SQS1_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_010G108900 PE=3 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 2.000e-133
Identity = 241/381 (63.25%), Postives = 300/381 (78.74%), Query Frame = 1


		  
Query: 18  KYAKSFTKHYNFQF----LVTRNPPSTLFGLQSSNLGVPRLSNQAQSLINSTP-ISEVPL 77
           KY  +F   Y        LV RNPP    G   S+L + R SNQ +S+I+ST  +S++PL
Sbjct: 13  KYTSNFPTRYTHLLYDLCLVPRNPPLMKKG-SFSDLTIVRSSNQTESMIDSTDKLSDIPL 72

Query: 78  FSREEAIERLRLSKERHKGKQQFLAMYCSIFGGITTDPAAMVIPMDDHMVHRGHGIFDTA 137
            S  EAIE+L+ ++E +KG QQ+LAMY SIFGGI T+ AAMVIPMDDHMVHRGHG+FDTA
Sbjct: 73  LSCLEAIEKLKRNRENNKGNQQYLAMYSSIFGGIVTEEAAMVIPMDDHMVHRGHGVFDTA 132

Query: 138 AIVDGHLYELDQHLDRCVRSATMAKIRLPFDSETMRNILIQTVIASKCKNGSLRYWLSAG 197
            I + HLYELDQH+DR V+SATMAKI LPFD ET++ ILIQTV  SKC NGSLRYW+SAG
Sbjct: 133 IIANRHLYELDQHVDRIVKSATMAKIILPFDRETIQRILIQTVSVSKCINGSLRYWISAG 192

Query: 198 YGNFLLSPSGCAEPTFYAIVIQTSASQPDHKGVKVITSSIPIKPPVFATMKSVNYLPNAL 257
            G+F LSPSGC +P  YAIVI+   S  D KG+KV+TSSIP+KPP FATMKSVNYLPNAL
Sbjct: 193 PGDFQLSPSGCHQPALYAIVIEDK-SLFDSKGIKVVTSSIPMKPPQFATMKSVNYLPNAL 252

Query: 258 CQMEAEENGCFTSVWLDTEGFVAEGPNMNVAFVTYDKELLMPRFDKVLGGCTLKRMRTLA 317
            +MEAEE G + ++WLD +GFVAEGP+MNVAF+T +KE+LMP+FDK+L GCT KR+ TLA
Sbjct: 253 SKMEAEEKGAYAAIWLDDDGFVAEGPSMNVAFITKEKEMLMPKFDKILNGCTAKRVFTLA 312

Query: 318 QELVKKGDIRGVSTRDVTVEEGKRAEEMMLFGSGVLVRPVVQWDDKIIGNGNEGPIARAL 377
           + LV++G +RG+   +V+V+EGK A+EMML GSGVLVRPV+QWD+++IG+G EGPI + L
Sbjct: 313 EGLVREGKLRGIRVDNVSVDEGKSADEMMLIGSGVLVRPVIQWDEQVIGDGKEGPITQTL 372

Query: 378 LDLLLEDMISGPSTVRTAVPY 394
           L+ +LEDM SGPS+VR  VPY
Sbjct: 373 LNFILEDMKSGPSSVRVPVPY 391

[Close]

BLAST of Spo25621.1 vs. ExPASy Swiss-Prot
Match: DAAA_ARATH (D-amino-acid transaminase, chloroplastic OS=Arabidopsis thaliana GN=DAAT PE=1 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 4.300e-121
Identity = 223/346 (64.45%), Postives = 270/346 (78.03%), Query Frame = 1


		  
Query: 53  RLSNQAQSLINSTPISE---VPLFSREEAIERLRLSKERHKGKQQFLAMYCSIFGGITTD 112
           RL+ +   L+ S   S+   VP+ S  E  ERL+L++    G QQFLAMY S+  GITTD
Sbjct: 34  RLTRRRTILMCSDSSSQSWNVPVLSSYEVGERLKLAR----GGQQFLAMYSSVVDGITTD 93

Query: 113 PAAMVIPMDDHMVHRGHGIFDTAAIVDGHLYELDQHLDRCVRSATMAKIRLPFDSETMRN 172
           PAAMV+P+DDHMVHRGHG+FDTA I++G+LYELDQHLDR +RSA+MAKI LPFD ET++ 
Sbjct: 94  PAAMVLPLDDHMVHRGHGVFDTALIINGYLYELDQHLDRILRSASMAKIPLPFDRETIKR 153

Query: 173 ILIQTVIASKCKNGSLRYWLSAGYGNFLLSPSGCAEPTFYAIVIQTS-ASQPDHKGVKVI 232
           ILIQTV  S C++GSLRYWLSAG G+FLLSPS C +PT YAIVI+T+ A  P   GVKV+
Sbjct: 154 ILIQTVSVSGCRDGSLRYWLSAGPGDFLLSPSQCLKPTLYAIVIKTNFAINP--IGVKVV 213

Query: 233 TSSIPIKPPVFATMKSVNYLPNALCQMEAEENGCFTSVWLDTEGFVAEGPNMNVAFVTY- 292
           TSSIPIKPP FAT+KSVNYLPN L QMEAE  G +  +W+  +GF+AEGPNMNVAFV   
Sbjct: 214 TSSIPIKPPEFATVKSVNYLPNVLSQMEAEAKGAYAGIWVCKDGFIAEGPNMNVAFVVNG 273

Query: 293 DKELLMPRFDKVLGGCTLKRMRTLAQELVKKGDIRGVSTRDVTVEEGKRAEEMMLFGSGV 352
            KEL+MPRFD VL GCT KR  TLA++LV KG ++ V   DVTVE+GK+A+EMML GSG+
Sbjct: 274 GKELVMPRFDNVLSGCTAKRTLTLAEQLVSKGILKTVKVMDVTVEDGKKADEMMLIGSGI 333

Query: 353 LVRPVVQWDDKIIGNGNEGPIARALLDLLLEDMISGPSTVRTAVPY 394
            +RPV+QWD++ IG G EGPIA+ALLDLLLEDM SGP +VR  VPY
Sbjct: 334 PIRPVIQWDEEFIGEGKEGPIAKALLDLLLEDMRSGPPSVRVLVPY 373

[Close]

BLAST of Spo25621.1 vs. ExPASy Swiss-Prot
Match: ILVE_METJA (Putative branched-chain-amino-acid aminotransferase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) GN=ilvE PE=3 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 2.700e-22
Identity = 71/256 (27.73%), Postives = 125/256 (48.83%), Query Frame = 1


		  
Query: 119 DHMVHRGHGIFDTAAIVDGHLYELDQHLDRCVRSATMAKIRLPFDSETMRNILIQTVIAS 178
           DH +  G G+F+     DG ++ L +H+DR   SA    I +P   E M +++++T+  +
Sbjct: 21  DHGLLYGDGVFEGIRAYDGVVFMLKEHIDRLYDSAKSLCIDIPLTKEEMIDVVLETLRVN 80

Query: 179 KCKNGSLRYWLSAGYGNFLLSPSGCAEPTFYAIVIQTSASQPDHKGVKVITSSIPIKP-- 238
             ++  +R  ++ G G+  L P  C +PT + I I       +  G++ IT S+   P  
Sbjct: 81  NLRDAYIRLVVTRGVGDLGLDPRKCGKPTIFCIAIPMPPLLGE-DGIRAITVSVRRLPVD 140

Query: 239 PVFATMKSVNYLPNALCQMEAEENGCFTSVWLDTEGFVAEGPNMNVAFVTYDKELLMPRF 298
            +   +KS+NYL + L +++A   G   +  LD +GFV EG   N+  V        P +
Sbjct: 141 VLNPAVKSLNYLNSVLAKIQANYAGVDEAFLLDDKGFVVEGTGDNIFIVKNGVLKTPPVY 200

Query: 299 DKVLGGCTLKRMRTLAQELVKKGDIRGVSTRDVTVEEGKRAEEMMLFGSGVLVRPVVQWD 358
             +L G T    R +  +L K+  I  V    +T+ +   A+E+ + G+   + PV + D
Sbjct: 201 QSILKGIT----RDVVIKLAKEEGIE-VVEEPLTLHDLYTADELFITGTAAEIVPVFEID 260

Query: 359 DKIIGNGNEGPIARAL 373
            ++I N   G I + L
Sbjct: 261 GRVINNKQVGEITKKL 270

[Close]

BLAST of Spo25621.1 vs. ExPASy Swiss-Prot
Match: ILVE_ARCFU (Putative branched-chain-amino-acid aminotransferase OS=Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) GN=ilvE PE=3 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 1.100e-20
Identity = 72/263 (27.38%), Postives = 124/263 (47.15%), Query Frame = 1


		  
Query: 119 DHMVHRGHGIFDTAAIVDGHLYELDQHLDRCVRSATMAKIRLPFDSETMRNILIQTVIAS 178
           DH    G G+F+     +G ++ L +H+DR   SA    + +P   E    I+++T+  +
Sbjct: 22  DHGFLYGDGVFEGIRAYNGRVFRLKEHIDRLYDSAKAIDLEIPITKEEFMEIILETLRKN 81

Query: 179 KCKNGSLRYWLSAGYGNFLLSPSGCAEPTFYAIVIQTSASQPD--HKGVKVIT-----SS 238
             ++  +R  ++ G G+  L P  C  P+   I         D   KG+  IT     +S
Sbjct: 82  NLRDAYIRPIVTRGIGDLGLDPRKCQNPSIIVITKPWGKLYGDLYEKGLTAITVAVRRNS 141

Query: 239 IPIKPPVFATMKSVNYLPNALCQMEAEENGCFTSVWLDTEGFVAEGPNMNVAFVTYDKEL 298
               PP    +KS+NYL N L ++EA   G   +++LD  G+V+EG   N+ FV  +  +
Sbjct: 142 FDALPP---NIKSLNYLNNILAKIEANAKGGDEAIFLDRNGYVSEGSGDNI-FVVKNGAI 201

Query: 299 LMPRFDKVLGGCTLKRMRTLAQELVKKGDIRGVSTRDVTVEEGKRAEEMMLFGSGVLVRP 358
             P     L G T    R    E++ +  I    T ++ + +   A+E+ + G+   + P
Sbjct: 202 TTPPTINNLRGIT----REAVIEIINRLGIPFKET-NIGLYDLYTADEVFVTGTAAEIAP 261

Query: 359 VVQWDDKIIGNGNEGPIARALLD 375
           +V  D + IG+G  G I R L++
Sbjct: 262 IVVIDGRKIGDGKPGEITRKLME 275

[Close]

BLAST of Spo25621.1 vs. ExPASy Swiss-Prot
Match: DAAA_BACLI (D-alanine aminotransferase OS=Bacillus licheniformis GN=dat PE=3 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 7.000e-15
Identity = 70/288 (24.31%), Postives = 127/288 (44.10%), Query Frame = 1


		  
Query: 102 IFGGITTDPAAMVIPMDDHMVHRGHGIFDTAAIVDGHLYELDQHLDRCVRSATMAKIRLP 161
           +F G   + +   + ++D     G G+++   I +G L+ LD+H+ R  +SA    I L 
Sbjct: 4   LFNGRLMERSECAVDIEDRGYQFGDGVYEVIRIYNGILFTLDEHIARLYKSAAEIGIDLS 63

Query: 162 FDSETMRNILIQTVIASKCKNGSLRYWLSAG-------YGNFLLSPSGCAEPTFYAIVIQ 221
           F    +++ L + V  ++ ++G L   ++ G       YG  L       + T Y   IQ
Sbjct: 64  FSEAELKSQLKELVDINQRRDGGLYLQVTRGKAPRKHQYGAGL-----TPQVTAYTFPIQ 123

Query: 222 TSASQPDHKGVKVITSSIPIKPPVFATMKSVNYLPNALCQMEAEENGCFTSVWLDTEGFV 281
               +    GV  IT+       +   +KS+N L N + + +A+E   F ++ L  +G V
Sbjct: 124 -KPEKEQQNGVSAITAD--DMRWLRCDIKSLNLLYNVMIKQKAQEASAFEAI-LIRDGLV 183

Query: 282 AEGPNMNVAFVTYDKELLMPRFDKVLGGCTLKRMRTLAQELVKKGDIRGVSTRDVTVEEG 341
            EG + NV     +     P    +L G T  ++  L +E     +      + VT +E 
Sbjct: 184 TEGTSSNVYVAKQNVIYTHPVTTLILNGITRMKVLQLCEE-----NGLNYEEKAVTKDEL 243

Query: 342 KRAEEMMLFGSGVLVRPVVQWDDKIIGNGNEGPIARALLDLLLEDMIS 383
             A+E+ +  +   V PV   D + IG+G  GP+ + +   L   ++S
Sbjct: 244 LNADEVFITSTTAEVIPVTSIDGQTIGSGAPGPLTKNVQTALQNSILS 277

[Close]

BLAST of Spo25621.1 vs. ExPASy Swiss-Prot
Match: DAAA_BACSU (D-alanine aminotransferase OS=Bacillus subtilis (strain 168) GN=dat PE=3 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 7.000e-15
Identity = 75/260 (28.85%), Postives = 115/260 (44.23%), Query Frame = 1


		  
Query: 115 IPMDDHMVHRGHGIFDTAAIVDGHLYELDQHLDRCVRSATMAKIRLPFDSETMRNILIQT 174
           I ++D     G GI++   +  G L+ L +H +R  RSA    I LPF  E +   L + 
Sbjct: 17  IDLEDRGYQFGDGIYEVIRVYKGVLFGLREHAERFFRSAAEIGISLPFSIEDLEWDLQKL 76

Query: 175 VIASKCKNGSLRYWLSAGYGNFLLSPSGCAEP--TFYAIVIQTSASQPDHKGVKVITSSI 234
           V  +    G++    + G            EP  T Y   ++    Q    GV  IT   
Sbjct: 77  VQENAVSEGAVYIQTTRGVAPRKHQYEAGLEPQTTAYTFTVK-KPEQEQAYGVAAITDED 136

Query: 235 PIKPPVFATMKSVNYLPNALCQMEAEENGCFTSVWLDTEGFVAEGPNMNVAFVTYDKELL 294
                +   +KS+N L N + +  A E G F ++ L  +G V EG + NV  V       
Sbjct: 137 --LRWLRCDIKSLNLLYNVMTKQRAYEAGAFEAILL-RDGVVTEGTSSNVYAVINGTVRT 196

Query: 295 MPRFDKVLGGCTLKRMRTLAQELVKKGDIRGVSTRDVTVEEGKRAEEMMLFGSGVLVRPV 354
            P    +L G T  RM  L   L++K  I+ +    V+ EE K+AEE+ +  +   + PV
Sbjct: 197 HPANRLILNGIT--RMNILG--LIEKNGIK-LDETPVSEEELKQAEEIFISSTTAEIIPV 256

Query: 355 VQWDDKIIGNGNEGPIARAL 373
           V  D + IG+G  GP+ + L
Sbjct: 257 VTLDGQSIGSGKPGPVTKQL 267

[Close]

BLAST of Spo25621.1 vs. TAIR (Arabidopsis)
Match: AT5G57850.1 (D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein)

HSP 1 Score: 436.0 bits (1120), Expect = 2.400e-122
Identity = 223/346 (64.45%), Postives = 270/346 (78.03%), Query Frame = 1


		  
Query: 53  RLSNQAQSLINSTPISE---VPLFSREEAIERLRLSKERHKGKQQFLAMYCSIFGGITTD 112
           RL+ +   L+ S   S+   VP+ S  E  ERL+L++    G QQFLAMY S+  GITTD
Sbjct: 34  RLTRRRTILMCSDSSSQSWNVPVLSSYEVGERLKLAR----GGQQFLAMYSSVVDGITTD 93

Query: 113 PAAMVIPMDDHMVHRGHGIFDTAAIVDGHLYELDQHLDRCVRSATMAKIRLPFDSETMRN 172
           PAAMV+P+DDHMVHRGHG+FDTA I++G+LYELDQHLDR +RSA+MAKI LPFD ET++ 
Sbjct: 94  PAAMVLPLDDHMVHRGHGVFDTALIINGYLYELDQHLDRILRSASMAKIPLPFDRETIKR 153

Query: 173 ILIQTVIASKCKNGSLRYWLSAGYGNFLLSPSGCAEPTFYAIVIQTS-ASQPDHKGVKVI 232
           ILIQTV  S C++GSLRYWLSAG G+FLLSPS C +PT YAIVI+T+ A  P   GVKV+
Sbjct: 154 ILIQTVSVSGCRDGSLRYWLSAGPGDFLLSPSQCLKPTLYAIVIKTNFAINP--IGVKVV 213

Query: 233 TSSIPIKPPVFATMKSVNYLPNALCQMEAEENGCFTSVWLDTEGFVAEGPNMNVAFVTY- 292
           TSSIPIKPP FAT+KSVNYLPN L QMEAE  G +  +W+  +GF+AEGPNMNVAFV   
Sbjct: 214 TSSIPIKPPEFATVKSVNYLPNVLSQMEAEAKGAYAGIWVCKDGFIAEGPNMNVAFVVNG 273

Query: 293 DKELLMPRFDKVLGGCTLKRMRTLAQELVKKGDIRGVSTRDVTVEEGKRAEEMMLFGSGV 352
            KEL+MPRFD VL GCT KR  TLA++LV KG ++ V   DVTVE+GK+A+EMML GSG+
Sbjct: 274 GKELVMPRFDNVLSGCTAKRTLTLAEQLVSKGILKTVKVMDVTVEDGKKADEMMLIGSGI 333

Query: 353 LVRPVVQWDDKIIGNGNEGPIARALLDLLLEDMISGPSTVRTAVPY 394
            +RPV+QWD++ IG G EGPIA+ALLDLLLEDM SGP +VR  VPY
Sbjct: 334 PIRPVIQWDEEFIGEGKEGPIAKALLDLLLEDMRSGPPSVRVLVPY 373

[Close]

BLAST of Spo25621.1 vs. TAIR (Arabidopsis)
Match: AT3G05190.1 (D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein)

HSP 1 Score: 76.3 bits (186), Expect = 4.800e-14
Identity = 67/263 (25.48%), Postives = 120/263 (45.63%), Query Frame = 1


		  
Query: 119 DHMVHRGHGIFDTAAIVDGHLYELDQHLDRCVRSATMAKIRLPFDSETMRNILIQTVIAS 178
           D +V  G  +++   I  G +++L++HLDR   SA      L FD+   R  + + +  +
Sbjct: 282 DSVVQGGDSVWEGLRIYKGKIFKLEEHLDRLFDSAKA----LAFDNVPAREEVKEAIFRT 341

Query: 179 KCKNG---SLRYWLSAGYGNFLLSPSGCAEPTFYAIVIQTSASQP----DHKGVKVITSS 238
              NG   +    LS   G  + S    A   +   +I  +  +P    +  G+ ++T++
Sbjct: 342 LITNGMFDNTHIRLSLTRGKKVTSGMSPAYNRYGCTLIVLAEWKPPVYDNEGGIVLVTAT 401

Query: 239 IPIKPP--VFATMKSVNYLPNALCQMEAEENGCFTSVWLDTEGFVAEGPNMNVAFVTYDK 298
                P  + + +   N L N L ++E+       ++ LD +G+V+E    N+ F+    
Sbjct: 402 TRRNSPNNLDSKIHHNNLLNNILAKIESNNTNAADAIMLDKDGYVSETNATNI-FMVKKG 461

Query: 299 ELLMPRFDKVLGGCTLKRMRTLAQELVKKGDIRGVSTRDVTVEEGKRAEEMMLFGSGVLV 358
            +L P  D  L G T    R    ELV K +   +  R +++ E   A E+   G+   +
Sbjct: 462 CVLTPHADYCLPGIT----RATVMELVVKENFI-LEERRISLSEFHTANEVWTTGTMGEL 521

Query: 359 RPVVQWDDKIIGNGNEGPIARAL 373
            PVV+ D ++IG+G  GP+ R L
Sbjct: 522 SPVVKIDGRVIGDGKVGPVTRTL 534

[Close]

BLAST of Spo25621.1 vs. TAIR (Arabidopsis)
Match: AT5G27410.1 (D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein)

HSP 1 Score: 76.3 bits (186), Expect = 4.800e-14
Identity = 68/261 (26.05%), Postives = 121/261 (46.36%), Query Frame = 1


		  
Query: 119 DHMVHRGHGIFDTAAIVDGHLYELDQHLDRCVRSATMAKIRLPFDSETMRNILIQTVIAS 178
           D +V  G  +++   I  G +++L++HLDR   SA           E ++  + +T+I +
Sbjct: 279 DSVVQGGDSVWEGLRIYKGKVFKLEEHLDRLSDSAKALAFNNVPTREEIKEAIFRTLITN 338

Query: 179 KC-KNGSLRYWLSAGYGNFLLSPSGCAEPTFYAIVIQTSASQP----DHKGVKVITSSIP 238
               N  +R  LS   G  + S    A   +   +I  +  +P    +  G+ ++T++  
Sbjct: 339 GMFDNTHIR--LSLTRGKKVTSGMSPAFNRYGCTLIVLAEWKPPVYDNDGGIVLVTATTR 398

Query: 239 IKPP--VFATMKSVNYLPNALCQMEAEENGCFTSVWLDTEGFVAEGPNMNVAFVTYDKEL 298
              P  + + +   N L N L ++E+       ++ LD +GFV+E    N+  V  D+ +
Sbjct: 399 RNSPNNLDSKIHHNNLLNNILAKIESNNANVDDAIMLDKDGFVSETNATNIFMVKKDR-V 458

Query: 299 LMPRFDKVLGGCTLKRMRTLAQELVKKGDIRGVSTRDVTVEEGKRAEEMMLFGSGVLVRP 358
           L P  D  L G T    R    ELV K +   +  R +++ E   A+E+   G+   + P
Sbjct: 459 LTPHADYCLPGIT----RATVMELVVKENFI-LEERRISLSEFHTADEVWTTGTMGELSP 518

Query: 359 VVQWDDKIIGNGNEGPIARAL 373
           VV+ D ++IG G  GP+ R L
Sbjct: 519 VVKIDGRVIGEGKVGPVTRRL 531

The following BLAST results are available for this feature:

BLAST of Spo25621.1 vs. NCBI nr
BLAST of Spo25621.1 vs. UniProtKB/TrEMBL
BLAST of Spo25621.1 vs. ExPASy Swiss-Prot
BLAST of Spo25621.1 vs. TAIR (Arabidopsis)

BLAST of Spo25621.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|870867103\|gb\|KMT18002.1\|	1.0e-162	74.8	hypothetical protein BVRB_2g03...	[more]
gi\|731317190\|ref\|XP_010668094.1\|	7.2e-161	73.8	PREDICTED: D-amino-acid transa...	[more]
gi\|902140766\|gb\|KNA04650.1\|	7.5e-142	87.8	hypothetical protein SOVF_1976...	[more]
gi\|743842006\|ref\|XP_011026599.1\|	2.1e-136	64.1	PREDICTED: D-amino-acid transa...	[more]
gi\|566213507\|ref\|XP_002324812.2\|	6.8e-135	63.8	hypothetical protein POPTR_001...	[more]

BLAST of Spo25621.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0J8CWP4_BETVU	7.0e-163	74.8	Uncharacterized protein OS=Bet...	[more]
A0A0K9QBM5_SPIOL	5.2e-142	87.8	Uncharacterized protein OS=Spi...	[more]
B9IKL7_POPTR	4.7e-135	63.8	Uncharacterized protein OS=Pop...	[more]
V4WBW3_9ROSI	1.5e-133	69.8	Uncharacterized protein OS=Cit...	[more]
A0A0D2SQS1_GOSRA	2.0e-133	63.2	Uncharacterized protein OS=Gos...	[more]

BLAST of Spo25621.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
DAAA_ARATH	4.3e-121	64.4	D-amino-acid transaminase, chl...	[more]
ILVE_METJA	2.7e-22	27.7	Putative branched-chain-amino-...	[more]
ILVE_ARCFU	1.1e-20	27.3	Putative branched-chain-amino-...	[more]
DAAA_BACLI	7.0e-15	24.3	D-alanine aminotransferase OS=...	[more]
DAAA_BACSU	7.0e-15	28.8	D-alanine aminotransferase OS=...	[more]

BLAST of Spo25621.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 3


Match Name	E-value	Identity	Description
AT5G57850.1	2.4e-122	64.4	D-aminoacid aminotransferase-l...	[more]
AT3G05190.1	4.8e-14	25.4	D-aminoacid aminotransferase-l...	[more]
AT5G27410.1	4.8e-14	26.0	D-aminoacid aminotransferase-l...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001544	Aminotransferase class IV	PANTHER	PTHR11825	SUBGROUP IIII AMINOTRANSFERASE	coord: 69..393 score: 5.5E
IPR001544	Aminotransferase class IV	PFAM	PF01063	Aminotran_4	coord: 127..354 score: 5.6
IPR001544	Aminotransferase class IV	unknown	SSF56752	D-aminoacid aminotransferase-like PLP-dependent enzymes	coord: 103..381 score: 1.7
None	No IPR available	GENE3D	3.20.10.10		coord: 222..379 score: 1.2
None	No IPR available	GENE3D	3.30.470.10		coord: 103..213 score: 4.3
None	No IPR available	PANTHER	PTHR11825:SF48	SUBFAMILY NOT NAMED	coord: 69..393 score: 5.5E

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006525	arginine metabolic process
biological_process	GO:0046436	D-alanine metabolic process
biological_process	GO:0005985	sucrose metabolic process
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0000272	polysaccharide catabolic process
biological_process	GO:0006457	protein folding
biological_process	GO:0010286	heat acclimation
biological_process	GO:0042967	obsolete acyl-carrier-protein biosynthetic process
biological_process	GO:0008152	metabolic process
biological_process	GO:0046654	tetrahydrofolate biosynthetic process
biological_process	GO:0006560	proline metabolic process
biological_process	GO:0009252	peptidoglycan biosynthetic process
biological_process	GO:0008153	para-aminobenzoic acid biosynthetic process
biological_process	GO:0006591	ornithine metabolic process
biological_process	GO:0009085	lysine biosynthetic process
biological_process	GO:0006558	L-phenylalanine metabolic process
cellular_component	GO:0009570	chloroplast stroma
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008696	4-amino-4-deoxychorismate lyase activity
molecular_function	GO:0047810	D-alanine:2-oxoglutarate aminotransferase activity
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0043531	ADP binding
molecular_function	GO:0008080	N-acetyltransferase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016161	beta-amylase activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo09712	0.68	Barchart \| Table
Spo09821	0.66	Barchart \| Table
Spo21193	0.66	Barchart \| Table
Spo01174	0.65	Barchart \| Table
Spo06783	0.65	Barchart \| Table