Spo25701 (gene)

Overview
NameSpo25701
Typegene
OrganismSpinacia oleracea (Spinach)
Description(1-aminocyclopropane-1-carboxylate oxidase-like protein) (Gibberellin 20 oxidase)
LocationSuper_scaffold_205 : 835614 .. 837869 (-)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTAATCCGCGATCCTTGGCATGAACCACGGGTCTCAGTTGTTGTGTTCTTCAACCCTGGCATGATGGAGAACTTGTATGGAACGATACCAGAGATAGTATCACCTGAAGAGCCTGCACAACCTTCAAATTATTCTAATGAGCTGTTAGAGCATGAGCTCTTTCTTGCTTCCTCGGTGCAAGATTTCAACGAAGGTATTCATTTACAAATGATTCTAGCTTGTTAAACTTGATTTCCCCATAACAATGAAGAATAGAGTGCCTTGATCTTGAAAGATCGAGGGTTATGTGCAGTATATGTGTATAGCATCTGTCACGTATGAGACACATATCTTTTTCCCTCCCCAATAGGTGTATAGTATCGATCTTGTATCTAGTCACTGCTCAGGTTAAGACTAAGACTTTAAACATCAGGATCATATTTATAAGATGAGGCCAGAGTAACAGAAATTATTCAAATAAAAAACAATTATAAACAGAGTGAAAAGTACATTTTCAGAAGGCATAAGGAAAGGAGAGAGAGAACAAGAGTAGGCTGTAGAGAGTAGGGAGTAATAGGGTGTGCAGAACCCTACATATAGTACAATAGGAATTATCAGGAGAATGTAGTTAAAAAATTGAAACAGAATTACTGATTTCTGACTAGCTAGTGATGATGTGACGTTGACGGGGTCTGATAGTGTATGGATCAAATGTATTACTGCAGTCTGTAGTCTGTAATCTGTTTATACAATACGTGTTCTTGTAGATTTTTCTCTTATGTGAATAAAATTTGATTGGTTGCCTTATTTTAGTCTTCGTTCTTGTTATGCAGAATGAATTAAATGGTTAATGCACTAAACATCGAGTTGTGAGGATGAAAATTGTTGTTCAAAAGCTGGAGTTGAAACAGGTGCAAGTGGGTATTAATTCCTGTCCGGCACCTGTTTCATCAGTAATCGACATTGGTTAAGTTTACAAATTTGGGTACTTCCTGAAAGTTCAATATTCACTCATTTTCTGAAAAACAGTGCATTGAATCCCTGGTCCAATGTGTTTATGTGGGTTATGTATGATCTCAAGTGTTCCTAAAGAATCTTAACCAGAAAACTCGCTAATGATCCTCTTTAGTTTCTATGGTACAGGCTTCTATTAGAATACGGTTGAATGAAATATCACTTGCTGATTTTTTTATACATGTTACATGTCTTTGCGAGGTTACATACAGCTTTTGTAGCACCTAGCTCTCGTGTCTTTGGAAGAGGTGAACTTGTATTAGATAGGAAGGAGATTGCTATGAGGTATATCAGATCAGGTTTCTTCATTGATCTCATTGCAGCTTTACCTTTTCCTCATATACAACTAATTCTTAGTTTTTTATTACCAAACATTAACATTTTAATGTCTACTACTTGTTATGGAAAGAAAATTGTTATGTTTTTTCGCTGCAGATTGTGATATTGTTTATAATTCCAGCAATTAAGCTGTCTAAATCAAGAATTGAAGCACCTATAATGCACTCCAATTGATTGTTTTCTTCCAATATATTCCACGGTCTTATCAGATATTCCCTTTGAGTGTTGAGATTATAAAGTCGGCTGGTGTTGTCACGAAAACAGCCTGGGCAGGAGCTGCTTATAATCTCATGTTCTACCAAGTGGATACAGATACAAGTATGTCCTATGATTTATGTTTATTGCAATTTTTATTTGTTTTTTAAAGTTTTCAAAAGTAATTGATTGTATTTTTCCTGTGTAGTGTCCTCGGCTAAAGAATGTCATGGATTCTTGATATTATGTCACGAGATACATGAAAGAGATCATCGACCATAAGCAAACTCGAATATCGGAAGATGTATGTATTTTTCTTTATTCAATTAAGCCTAGACATTAGTATATGCATTGTCACTAATTATGTTAATTTTATCATTTTGTAGTACTTTCATGATTGCAATATTCCGAATTACGATCAAGGGCAAATTGATGAAGTCATAGCTCAATGGACGCGTCATGTTCTTCTCGTGTGAATATTGTGATGTATTAATTATGTTTTTCGACTAGAATTTCTCTAGAACGTTTTAATTTTATTTTATTGGTTTGAAATTAAAAGAAGTACTATATGACAACGATACTTCTTTCTGTTTTCTAGTGTGTTTTTAGTCTACTTGTATTTAGGCATATATGATTTTAAGTTATGCACGAGACATCGAGACGTTTATTGATATGATAGAAATGACGTTGGATCTTATATATATTCAATTTGATTACTTTGTGTAG

mRNA sequence

ATGCTAATCCGCGATCCTTGGCATGAACCACGGGTCTCAGTTGTTGTGTTCTTCAACCCTGGCATGATGGAGAACTTGTATGGAACGATACCAGAGATAGTATCACCTGAAGAGCCTGCACAACCTTCAAATTATTCTAATGAGCTGTTAGAGCATGAGCTCTTTCTTGCTTCCTCGGTGCAAGATTTCAACGAAGAATGAATTAAATGGTTAATGCACTAAACATCGAGTTGTGAGGATGAAAATTGTTGTTCAAAAGCTGGAGTTGAAACAGGTGCAAGTGGGTATTAATTCCTGTCCGGCACCTGTTTCATCAGTAATCGACATTGGTTAAGTTTACAAATTTGGCTTTTGTAGCACCTAGCTCTCGTGTCTTTGGAAGAGGTGAACTTGTATTAGATAGGAAGGAGATTGCTATGAGATTGTGATATTGTTTATAATTCCAGCAATTAAGCTGTCTAAATCAAGAATTGAAGCACCTATAATGCACTCCAATTGATTGTTTTCTTCCAATATATTCCACGGTCTTATCAGATATTCCCTTTGAGTGTTGAGATTATAAAGTCGGCTGGTGTTGTCACGAAAACAGCCTGGGCAGGAGCTGCTTATAATCTCATGTTCTACCAAGTGGATACAGATACAATGTCCTCGGCTAAAGAATGTCATGGATTCTTGATATTATGTCACGAGATACATGAAAGAGATCATCGACCATAAGCAAACTCGAATATCGGAAGATTACTTTCATGATTGCAATATTCCGAATTACGATCAAGGGCAAATTGATGAAGTCATAGCTCAATGGACGCGTCATGTTCTTCTCGTGTGAATATTGTGATGTATTAATTATGTTTTTCGACTAGAATTTCTCTAGAACGTTTTAATTTTATTTTATTGGTTTGAAATTAAAAGAAGTACTATATGACAACGATACTTCTTTCTGTTTTCTAGTGTGTTTTTAGTCTACTTGTATTTAGGCATATATGATTTTAAGTTATGCACGAGACATCGAGACGTTTATTGATATGATAGAAATGACGTTGGATCTTATATATATTCAATTTGATTACTTTGTGTAG

Coding sequence (CDS)

ATGCTAATCCGCGATCCTTGGCATGAACCACGGGTCTCAGTTGTTGTGTTCTTCAACCCTGGCATGATGGAGAACTTGTATGGAACGATACCAGAGATAGTATCACCTGAAGAGCCTGCACAACCTTCAAATTATTCTAATGAGCTGTTAGAGCATGAGCTCTTTCTTGCTTCCTCGGTGCAAGATTTCAACGAAGAATGA

Protein sequence

MLIRDPWHEPRVSVVVFFNPGMMENLYGTIPEIVSPEEPAQPSNYSNELLEHELFLASSVQDFNEE
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo25701.1Spo25701.1mRNA


Homology
[Close]
BLAST of Spo25701.1 vs. NCBI nr
Match: gi|902209559|gb|KNA16231.1| (hypothetical protein SOVF_090750 [Spinacia oleracea])

HSP 1 Score: 117.5 bits (293), Expect = 9.000e-24
Identity = 55/61 (90.16%), Postives = 57/61 (93.44%), Query Frame = 1 

		  

Query: 5   DPWHEPRVSVVVFFNPGMMENLYGTIPEIVSPEEPAQPSNYSNELLEHELFLASSVQDFN 64
           +PWHEPRVSVVVFFNP MMENLYG IPEIVSPEEPAQPSNYSNE LE ELFLASSVQDFN
Sbjct: 91  NPWHEPRVSVVVFFNPSMMENLYGPIPEIVSPEEPAQPSNYSNEQLERELFLASSVQDFN 150

Query: 65  E 66
           +
Sbjct: 151 Q 151
[Close]
BLAST of Spo25701.1 vs. NCBI nr
Match: gi|902237995|gb|KNA25000.1| (hypothetical protein SOVF_010500 [Spinacia oleracea])

HSP 1 Score: 63.2 bits (152), Expect = 2.000e-7
Identity = 27/36 (75.00%), Postives = 30/36 (83.33%), Query Frame = 1 

		  

Query: 5   DPWHEPRVSVVVFFNPGMMENLYGTIPEIVSPEEPA 41
           +P HEPRVS+ VFFNP M +NLYG IPE VSPEEPA
Sbjct: 301 NPQHEPRVSIAVFFNPSMRDNLYGPIPETVSPEEPA 336
[Close]
BLAST of Spo25701.1 vs. NCBI nr
Match: gi|731361633|ref|XP_010692464.1| (PREDICTED: 1-aminocyclopropane-1-carboxylate oxidase homolog 4-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 62.8 bits (151), Expect = 2.600e-7
Identity = 34/67 (50.75%), Postives = 42/67 (62.69%), Query Frame = 1 

		  

Query: 5   DPWHEPRVSVVVFFNPGMMENLYGTIPEIVSPEEPAQP-----SNYSNELLEHELFLASS 64
           +P HEPRVS+ VFFNPG+ ENLYG +PEIVS EEPA       +N+     + EL   S 
Sbjct: 276 NPLHEPRVSIAVFFNPGVRENLYGPLPEIVSAEEPAHYQQFTFTNFITRFFKKELDGKSL 335

Query: 65  VQDFNEE 67
           V  F+ E
Sbjct: 336 VNYFSLE 342
[Close]
BLAST of Spo25701.1 vs. NCBI nr
Match: gi|870847771|gb|KMT00119.1| (hypothetical protein BVRB_1g019420 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 62.8 bits (151), Expect = 2.600e-7
Identity = 34/67 (50.75%), Postives = 42/67 (62.69%), Query Frame = 1 

		  

Query: 5   DPWHEPRVSVVVFFNPGMMENLYGTIPEIVSPEEPAQP-----SNYSNELLEHELFLASS 64
           +P HEPRVS+ VFFNPG+ ENLYG +PEIVS EEPA       +N+     + EL   S 
Sbjct: 301 NPLHEPRVSIAVFFNPGVRENLYGPLPEIVSAEEPAHYQQFTFTNFITRFFKKELDGKSL 360

Query: 65  VQDFNEE 67
           V  F+ E
Sbjct: 361 VNYFSLE 367
[Close]
BLAST of Spo25701.1 vs. NCBI nr
Match: gi|731361299|ref|XP_010692279.1| (PREDICTED: 1-aminocyclopropane-1-carboxylate oxidase homolog 4-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 62.4 bits (150), Expect = 3.400e-7
Identity = 27/36 (75.00%), Postives = 30/36 (83.33%), Query Frame = 1 

		  

Query: 5   DPWHEPRVSVVVFFNPGMMENLYGTIPEIVSPEEPA 41
           +P HEPRVSV VFFNP M ENLYG +PE+VS EEPA
Sbjct: 296 NPLHEPRVSVAVFFNPSMRENLYGPLPEVVSAEEPA 331
[Close]
BLAST of Spo25701.1 vs. UniProtKB/TrEMBL
Match: A0A0K9R9P7_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_090750 PE=4 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 6.300e-24
Identity = 55/61 (90.16%), Postives = 57/61 (93.44%), Query Frame = 1 

		  

Query: 5   DPWHEPRVSVVVFFNPGMMENLYGTIPEIVSPEEPAQPSNYSNELLEHELFLASSVQDFN 64
           +PWHEPRVSVVVFFNP MMENLYG IPEIVSPEEPAQPSNYSNE LE ELFLASSVQDFN
Sbjct: 91  NPWHEPRVSVVVFFNPSMMENLYGPIPEIVSPEEPAQPSNYSNEQLERELFLASSVQDFN 150

Query: 65  E 66
           +
Sbjct: 151 Q 151
[Close]
BLAST of Spo25701.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RZS0_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_010500 PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.400e-7
Identity = 27/36 (75.00%), Postives = 30/36 (83.33%), Query Frame = 1 

		  

Query: 5   DPWHEPRVSVVVFFNPGMMENLYGTIPEIVSPEEPA 41
           +P HEPRVS+ VFFNP M +NLYG IPE VSPEEPA
Sbjct: 301 NPQHEPRVSIAVFFNPSMRDNLYGPIPETVSPEEPA 336
[Close]
BLAST of Spo25701.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BJ88_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_1g019420 PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 1.800e-7
Identity = 34/67 (50.75%), Postives = 42/67 (62.69%), Query Frame = 1 

		  

Query: 5   DPWHEPRVSVVVFFNPGMMENLYGTIPEIVSPEEPAQP-----SNYSNELLEHELFLASS 64
           +P HEPRVS+ VFFNPG+ ENLYG +PEIVS EEPA       +N+     + EL   S 
Sbjct: 301 NPLHEPRVSIAVFFNPGVRENLYGPLPEIVSAEEPAHYQQFTFTNFITRFFKKELDGKSL 360

Query: 65  VQDFNEE 67
           V  F+ E
Sbjct: 361 VNYFSLE 367
[Close]
BLAST of Spo25701.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BJ89_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_1g019410 PE=3 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 2.400e-7
Identity = 27/36 (75.00%), Postives = 30/36 (83.33%), Query Frame = 1 

		  

Query: 5   DPWHEPRVSVVVFFNPGMMENLYGTIPEIVSPEEPA 41
           +P HEPRVSV VFFNP M ENLYG +PE+VS EEPA
Sbjct: 296 NPLHEPRVSVAVFFNPSMRENLYGPLPEVVSAEEPA 331
[Close]
BLAST of Spo25701.1 vs. UniProtKB/TrEMBL
Match: A0A0V0HU63_SOLCH (Putative 1-aminocyclopropane-1-carboxylate oxidase-like OS=Solanum chacoense PE=3 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 4.500e-6
Identity = 22/39 (56.41%), Postives = 32/39 (82.05%), Query Frame = 1 

		  

Query: 2   LIRDPWHEPRVSVVVFFNPGMMENLYGTIPEIVSPEEPA 41
           ++ +P+HEPRVSV +FFNPG  EN +G +PE++S E+PA
Sbjct: 172 VLANPFHEPRVSVAIFFNPGQRENSFGPLPELISDEKPA 210
The following BLAST results are available for this feature:
BLAST of Spo25701.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902209559|gb|KNA16231.1|9.0e-2490.1hypothetical protein SOVF_0907... [more]
gi|902237995|gb|KNA25000.1|2.0e-775.0hypothetical protein SOVF_0105... [more]
gi|731361633|ref|XP_010692464.1|2.6e-750.7PREDICTED: 1-aminocyclopropane... [more]
gi|870847771|gb|KMT00119.1|2.6e-750.7hypothetical protein BVRB_1g01... [more]
gi|731361299|ref|XP_010692279.1|3.4e-775.0PREDICTED: 1-aminocyclopropane... [more]
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BLAST of Spo25701.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9R9P7_SPIOL6.3e-2490.1Uncharacterized protein OS=Spi... [more]
A0A0K9RZS0_SPIOL1.4e-775.0Uncharacterized protein OS=Spi... [more]
A0A0J8BJ88_BETVU1.8e-750.7Uncharacterized protein OS=Bet... [more]
A0A0J8BJ89_BETVU2.4e-775.0Uncharacterized protein OS=Bet... [more]
A0A0V0HU63_SOLCH4.5e-656.4Putative 1-aminocyclopropane-1... [more]
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BLAST of Spo25701.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Spo25701.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 0
Match NameE-valueIdentityDescription
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GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001101 response to acid chemical
biological_process GO:0071806 protein transmembrane transport
biological_process GO:0006950 response to stress
biological_process GO:0006189 'de novo' IMP biosynthetic process
biological_process GO:0006810 transport
biological_process GO:0048868 pollen tube development
biological_process GO:0009628 response to abiotic stimulus
biological_process GO:0034655 nucleobase-containing compound catabolic process
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0006260 DNA replication
biological_process GO:0006281 DNA repair
biological_process GO:0044265 cellular macromolecule catabolic process
biological_process GO:0009737 response to abscisic acid
biological_process GO:0016192 vesicle-mediated transport
biological_process GO:0009113 purine nucleobase biosynthetic process
biological_process GO:1901700 response to oxygen-containing compound
cellular_component GO:0005744 mitochondrial inner membrane presequence translocase complex
cellular_component GO:0009941 chloroplast envelope
cellular_component GO:0009527 plastid outer membrane
cellular_component GO:0005739 mitochondrion
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0044444 cytoplasmic part
cellular_component GO:0009570 chloroplast stroma
cellular_component GO:0031981 nuclear lumen
molecular_function GO:0046872 metal ion binding
molecular_function GO:0015450 P-P-bond-hydrolysis-driven protein transmembrane transporter activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0004527 exonuclease activity
molecular_function GO:0004519 endonuclease activity
molecular_function GO:0004637 phosphoribosylamine-glycine ligase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0005515 protein binding
RNA-Seq Expression