Spo25805 (gene) | SpinachBase

Overview


Name	Spo25805
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Tic20 family protein Ycf60
Location	chr4 : 11597632 .. 11598303 (+)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCTCAATCCCACTCCTCCGCCTCTCCCACCTCCCAATCACCACCGCAACAACCACCACACACCACCACAAATACACCCAAATTCGCCCTCTCTCCCTCCTCTACCACCCACTAAAATCACCCCCCACCACCAAAACCCCCTTCCAAATCACCCCCAAAAAACCCAAAACCCTAAAACCCAATCAAAAACCCATTTCCATGTCCGCAACAACCCCAGCAACAGACCGCTTAATCTCCTCCATCTCCTACTTCCTCCCCTTCGTCAATGGCCTCCATTACGGCCGCTTCCTCTTCGCTCAATACCCACCACTCTCTCTCCTCTTCGAACCCATCTTACCCTTTCTCTCTCTCTACAAATCAATCCCTTATGCCAGCTTCGTCTCCTTCTTCGGCCTCTACCTCGGCGTTGTCAGAAACCCTAAATTCTCGAACTACGTGAGATTCAACGCAATGCAGGCCCTTGTTCTTGATGTTCTCCTCGTTCTTCCTTTGCTTTTGCAGCGGATTTTCACCCCTGGGAAAGGGCTAGGGTTTAAGATTATGGTGATGGGTCATAATTTCTTGTTTGTGTTCATTGTTTCTTGTTTTGTTTATGGGGTTGTTTCTTCTGTTTTGGGGAAAACTCCTTATTTGCCTCTTGTTGCTGATGCTGCTGGCAGACAAATGTGA

mRNA sequence

ATGGCCTCAATCCCACTCCTCCGCCTCTCCCACCTCCCAATCACCACCGCAACAACCACCACACACCACCACAAATACACCCAAATTCGCCCTCTCTCCCTCCTCTACCACCCACTAAAATCACCCCCCACCACCAAAACCCCCTTCCAAATCACCCCCAAAAAACCCAAAACCCTAAAACCCAATCAAAAACCCATTTCCATGTCCGCAACAACCCCAGCAACAGACCGCTTAATCTCCTCCATCTCCTACTTCCTCCCCTTCGTCAATGGCCTCCATTACGGCCGCTTCCTCTTCGCTCAATACCCACCACTCTCTCTCCTCTTCGAACCCATCTTACCCTTTCTCTCTCTCTACAAATCAATCCCTTATGCCAGCTTCGTCTCCTTCTTCGGCCTCTACCTCGGCGTTGTCAGAAACCCTAAATTCTCGAACTACGTGAGATTCAACGCAATGCAGGCCCTTGTTCTTGATGTTCTCCTCGTTCTTCCTTTGCTTTTGCAGCGGATTTTCACCCCTGGGAAAGGGCTAGGGTTTAAGATTATGGTGATGGGTCATAATTTCTTGTTTGTGTTCATTGTTTCTTGTTTTGTTTATGGGGTTGTTTCTTCTGTTTTGGGGAAAACTCCTTATTTGCCTCTTGTTGCTGATGCTGCTGGCAGACAAATGTGA

Coding sequence (CDS)

ATGGCCTCAATCCCACTCCTCCGCCTCTCCCACCTCCCAATCACCACCGCAACAACCACCACACACCACCACAAATACACCCAAATTCGCCCTCTCTCCCTCCTCTACCACCCACTAAAATCACCCCCCACCACCAAAACCCCCTTCCAAATCACCCCCAAAAAACCCAAAACCCTAAAACCCAATCAAAAACCCATTTCCATGTCCGCAACAACCCCAGCAACAGACCGCTTAATCTCCTCCATCTCCTACTTCCTCCCCTTCGTCAATGGCCTCCATTACGGCCGCTTCCTCTTCGCTCAATACCCACCACTCTCTCTCCTCTTCGAACCCATCTTACCCTTTCTCTCTCTCTACAAATCAATCCCTTATGCCAGCTTCGTCTCCTTCTTCGGCCTCTACCTCGGCGTTGTCAGAAACCCTAAATTCTCGAACTACGTGAGATTCAACGCAATGCAGGCCCTTGTTCTTGATGTTCTCCTCGTTCTTCCTTTGCTTTTGCAGCGGATTTTCACCCCTGGGAAAGGGCTAGGGTTTAAGATTATGGTGATGGGTCATAATTTCTTGTTTGTGTTCATTGTTTCTTGTTTTGTTTATGGGGTTGTTTCTTCTGTTTTGGGGAAAACTCCTTATTTGCCTCTTGTTGCTGATGCTGCTGGCAGACAAATGTGA

Protein sequence

MASIPLLRLSHLPITTATTTTHHHKYTQIRPLSLLYHPLKSPPTTKTPFQITPKKPKTLKPNQKPISMSATTPATDRLISSISYFLPFVNGLHYGRFLFAQYPPLSLLFEPILPFLSLYKSIPYASFVSFFGLYLGVVRNPKFSNYVRFNAMQALVLDVLLVLPLLLQRIFTPGKGLGFKIMVMGHNFLFVFIVSCFVYGVVSSVLGKTPYLPLVADAAGRQM

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo25805.1	Spo25805.1	mRNA

Homology

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BLAST of Spo25805.1 vs. NCBI nr
Match: gi|731326196|ref|XP_010673897.1| (PREDICTED: protein TIC 20-II, chloroplastic [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 337.8 bits (865), Expect = 1.400e-89
Identity = 183/225 (81.33%), Postives = 197/225 (87.56%), Query Frame = 1

		  
Query: 1   MASIPLLRLSHLPITTATTTTHHHKYTQIRPLSLLYHPLKSPPTTKTPFQITPKKPKTLK 60
           MASIPLLRLS LP TT TTT +H + T   PLSLL+HPLKSPP TKT F I+P KPKTLK
Sbjct: 1   MASIPLLRLSLLPSTTTTTTLNHRRKTLTPPLSLLFHPLKSPPITKTQFPISP-KPKTLK 60

Query: 61  PNQKPISM--SATTPATDRLISSISYFLPFVNGLHYGRFLFAQYPPLSLLFEPILPFLSL 120
             QK ISM  S+T PAT+RLISSISYFLPF+NGLHYGRFL AQ+PPLSL FEP LPF SL
Sbjct: 61  -TQKLISMSSSSTVPATERLISSISYFLPFLNGLHYGRFLLAQFPPLSLFFEPFLPFFSL 120

Query: 121 YKSIPYASFVSFFGLYLGVVRNPKFSNYVRFNAMQALVLDVLLVLPLLLQRIFTPGKGLG 180
           YKSIPYASFVSFF +YLGVVRNP FS+YVRFNAMQALVLDVLLVLPLLLQ+IFTPGKGLG
Sbjct: 121 YKSIPYASFVSFFAIYLGVVRNPSFSHYVRFNAMQALVLDVLLVLPLLLQKIFTPGKGLG 180

Query: 181 FKIMVMGHNFLFVFIVSCFVYGVVSSVLGKTPYLPLVADAAGRQM 224
           FKIMVM HNFLFVF+VSCFVYGVV S+LGKTPYLP VADAAGRQ+
Sbjct: 181 FKIMVMCHNFLFVFVVSCFVYGVVCSLLGKTPYLPFVADAAGRQL 223

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BLAST of Spo25805.1 vs. NCBI nr
Match: gi|902229084|gb|KNA21374.1| (hypothetical protein SOVF_043780 [Spinacia oleracea])

HSP 1 Score: 304.3 bits (778), Expect = 1.800e-79
Identity = 156/156 (100.00%), Postives = 156/156 (100.00%), Query Frame = 1

		  
Query: 68  MSATTPATDRLISSISYFLPFVNGLHYGRFLFAQYPPLSLLFEPILPFLSLYKSIPYASF 127
           MSATTPATDRLISSISYFLPFVNGLHYGRFLFAQYPPLSLLFEPILPFLSLYKSIPYASF
Sbjct: 1   MSATTPATDRLISSISYFLPFVNGLHYGRFLFAQYPPLSLLFEPILPFLSLYKSIPYASF 60

Query: 128 VSFFGLYLGVVRNPKFSNYVRFNAMQALVLDVLLVLPLLLQRIFTPGKGLGFKIMVMGHN 187
           VSFFGLYLGVVRNPKFSNYVRFNAMQALVLDVLLVLPLLLQRIFTPGKGLGFKIMVMGHN
Sbjct: 61  VSFFGLYLGVVRNPKFSNYVRFNAMQALVLDVLLVLPLLLQRIFTPGKGLGFKIMVMGHN 120

Query: 188 FLFVFIVSCFVYGVVSSVLGKTPYLPLVADAAGRQM 224
           FLFVFIVSCFVYGVVSSVLGKTPYLPLVADAAGRQM
Sbjct: 121 FLFVFIVSCFVYGVVSSVLGKTPYLPLVADAAGRQM 156

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BLAST of Spo25805.1 vs. NCBI nr
Match: gi|870863562|gb|KMT14726.1| (hypothetical protein BVRB_4g074910 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 271.9 bits (694), Expect = 9.700e-70
Identity = 136/158 (86.08%), Postives = 147/158 (93.04%), Query Frame = 1

		  
Query: 66  ISMSATTPATDRLISSISYFLPFVNGLHYGRFLFAQYPPLSLLFEPILPFLSLYKSIPYA 125
           +S S+T PAT+RLISSISYFLPF+NGLHYGRFL AQ+PPLSL FEP LPF SLYKSIPYA
Sbjct: 1   MSSSSTVPATERLISSISYFLPFLNGLHYGRFLLAQFPPLSLFFEPFLPFFSLYKSIPYA 60

Query: 126 SFVSFFGLYLGVVRNPKFSNYVRFNAMQALVLDVLLVLPLLLQRIFTPGKGLGFKIMVMG 185
           SFVSFF +YLGVVRNP FS+YVRFNAMQALVLDVLLVLPLLLQ+IFTPGKGLGFKIMVM 
Sbjct: 61  SFVSFFAIYLGVVRNPSFSHYVRFNAMQALVLDVLLVLPLLLQKIFTPGKGLGFKIMVMC 120

Query: 186 HNFLFVFIVSCFVYGVVSSVLGKTPYLPLVADAAGRQM 224
           HNFLFVF+VSCFVYGVV S+LGKTPYLP VADAAGRQ+
Sbjct: 121 HNFLFVFVVSCFVYGVVCSLLGKTPYLPFVADAAGRQL 158

[Close]

BLAST of Spo25805.1 vs. NCBI nr
Match: gi|703116818|ref|XP_010101228.1| (hypothetical protein L484_005577 [Morus notabilis])

HSP 1 Score: 236.9 bits (603), Expect = 3.400e-59
Identity = 139/227 (61.23%), Postives = 165/227 (72.69%), Query Frame = 1

		  
Query: 1   MASIPL--LRLSHLPIT-TATTTTHHHKYTQIRPLSLLYHPLKSPPTTKTPFQITPKKPK 60
           MASIP+  L   H+  T T  T       + I P SLL HPL    T +T +Q  PK   
Sbjct: 1   MASIPIQILHFHHVSATETPKTLAFKSPKSAISP-SLLPHPLLR--THQTKWQAKPKNST 60

Query: 61  TLKPNQKPISMSATTPATDRLISSISYFLPFVNGLHYGRFLFAQYPPLSLLFEPILPFLS 120
                 + +S S TTPATDRLIS+++Y LPF N L YGR+LF QYP L LLF+P+LP +S
Sbjct: 61  LTGYGIRMMSSSTTTPATDRLISAVAYSLPFFNSLQYGRYLFLQYPKLGLLFDPLLPIIS 120

Query: 121 LYKSIPYASFVSFFGLYLGVVRNPKFSNYVRFNAMQALVLDVLLVLPLLLQRIFTPGK-G 180
           LY+SIPY+SFV+FF LYLGVVRNP FS YVRFN+MQA+ LDVLLVLPLL+QRIF+PG+ G
Sbjct: 121 LYRSIPYSSFVAFFALYLGVVRNPSFSRYVRFNSMQAVTLDVLLVLPLLVQRIFSPGRSG 180

Query: 181 LGFKIMVMGHNFLFVFIVSCFVYGVVSSVLGKTPYLPLVADAAGRQM 224
           LGF+IMV  H+ LFVF V CFVYG  SSVLG+TPYLP VADAAGRQ+
Sbjct: 181 LGFRIMVWVHSALFVFSVLCFVYGSASSVLGRTPYLPFVADAAGRQI 224

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BLAST of Spo25805.1 vs. NCBI nr
Match: gi|764596660|ref|XP_011466077.1| (PREDICTED: protein TIC 20-II, chloroplastic isoform X1 [Fragaria vesca subsp. vesca])

HSP 1 Score: 233.8 bits (595), Expect = 2.900e-58
Identity = 121/168 (72.02%), Postives = 139/168 (82.74%), Query Frame = 1

		  
Query: 60  KPNQKPI---SMSATTPATDRLISSISYFLPFVNGLHYGRFLFAQYPPLSLLFEPILPFL 119
           KP  K I   S    TPATDRLIS++SY LPF N L YGR+LF Q+P L LLFEP+LP L
Sbjct: 37  KPKSKTITCMSSGTPTPATDRLISAVSYTLPFFNSLQYGRYLFLQFPKLGLLFEPLLPLL 96

Query: 120 SLYKSIPYASFVSFFGLYLGVVRNPKFSNYVRFNAMQALVLDVLLVLPLLLQRIFTPGK- 179
            LY+SIPY+SFV+FF LYLGVVRNP FS+Y RFNAMQA+ LDVLLVLPLL+QRIF+PG+ 
Sbjct: 97  GLYRSIPYSSFVAFFALYLGVVRNPSFSHYARFNAMQAVTLDVLLVLPLLVQRIFSPGQA 156

Query: 180 GLGFKIMVMGHNFLFVFIVSCFVYGVVSSVLGKTPYLPLVADAAGRQM 224
           GLGFK+MV GHN +FVF V CFVYG+VSSVLG+TPYLP VADAAGRQ+
Sbjct: 157 GLGFKLMVWGHNGIFVFSVFCFVYGLVSSVLGRTPYLPFVADAAGRQL 204

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BLAST of Spo25805.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RR52_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_043780 PE=4 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 1.200e-79
Identity = 156/156 (100.00%), Postives = 156/156 (100.00%), Query Frame = 1

		  
Query: 68  MSATTPATDRLISSISYFLPFVNGLHYGRFLFAQYPPLSLLFEPILPFLSLYKSIPYASF 127
           MSATTPATDRLISSISYFLPFVNGLHYGRFLFAQYPPLSLLFEPILPFLSLYKSIPYASF
Sbjct: 1   MSATTPATDRLISSISYFLPFVNGLHYGRFLFAQYPPLSLLFEPILPFLSLYKSIPYASF 60

Query: 128 VSFFGLYLGVVRNPKFSNYVRFNAMQALVLDVLLVLPLLLQRIFTPGKGLGFKIMVMGHN 187
           VSFFGLYLGVVRNPKFSNYVRFNAMQALVLDVLLVLPLLLQRIFTPGKGLGFKIMVMGHN
Sbjct: 61  VSFFGLYLGVVRNPKFSNYVRFNAMQALVLDVLLVLPLLLQRIFTPGKGLGFKIMVMGHN 120

Query: 188 FLFVFIVSCFVYGVVSSVLGKTPYLPLVADAAGRQM 224
           FLFVFIVSCFVYGVVSSVLGKTPYLPLVADAAGRQM
Sbjct: 121 FLFVFIVSCFVYGVVSSVLGKTPYLPLVADAAGRQM 156

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BLAST of Spo25805.1 vs. UniProtKB/TrEMBL
Match: A0A0J8FFX6_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_4g074910 PE=4 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 6.700e-70
Identity = 136/158 (86.08%), Postives = 147/158 (93.04%), Query Frame = 1

		  
Query: 66  ISMSATTPATDRLISSISYFLPFVNGLHYGRFLFAQYPPLSLLFEPILPFLSLYKSIPYA 125
           +S S+T PAT+RLISSISYFLPF+NGLHYGRFL AQ+PPLSL FEP LPF SLYKSIPYA
Sbjct: 1   MSSSSTVPATERLISSISYFLPFLNGLHYGRFLLAQFPPLSLFFEPFLPFFSLYKSIPYA 60

Query: 126 SFVSFFGLYLGVVRNPKFSNYVRFNAMQALVLDVLLVLPLLLQRIFTPGKGLGFKIMVMG 185
           SFVSFF +YLGVVRNP FS+YVRFNAMQALVLDVLLVLPLLLQ+IFTPGKGLGFKIMVM 
Sbjct: 61  SFVSFFAIYLGVVRNPSFSHYVRFNAMQALVLDVLLVLPLLLQKIFTPGKGLGFKIMVMC 120

Query: 186 HNFLFVFIVSCFVYGVVSSVLGKTPYLPLVADAAGRQM 224
           HNFLFVF+VSCFVYGVV S+LGKTPYLP VADAAGRQ+
Sbjct: 121 HNFLFVFVVSCFVYGVVCSLLGKTPYLPFVADAAGRQL 158

[Close]

BLAST of Spo25805.1 vs. UniProtKB/TrEMBL
Match: W9RQC5_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_005577 PE=4 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 2.400e-59
Identity = 139/227 (61.23%), Postives = 165/227 (72.69%), Query Frame = 1

		  
Query: 1   MASIPL--LRLSHLPIT-TATTTTHHHKYTQIRPLSLLYHPLKSPPTTKTPFQITPKKPK 60
           MASIP+  L   H+  T T  T       + I P SLL HPL    T +T +Q  PK   
Sbjct: 1   MASIPIQILHFHHVSATETPKTLAFKSPKSAISP-SLLPHPLLR--THQTKWQAKPKNST 60

Query: 61  TLKPNQKPISMSATTPATDRLISSISYFLPFVNGLHYGRFLFAQYPPLSLLFEPILPFLS 120
                 + +S S TTPATDRLIS+++Y LPF N L YGR+LF QYP L LLF+P+LP +S
Sbjct: 61  LTGYGIRMMSSSTTTPATDRLISAVAYSLPFFNSLQYGRYLFLQYPKLGLLFDPLLPIIS 120

Query: 121 LYKSIPYASFVSFFGLYLGVVRNPKFSNYVRFNAMQALVLDVLLVLPLLLQRIFTPGK-G 180
           LY+SIPY+SFV+FF LYLGVVRNP FS YVRFN+MQA+ LDVLLVLPLL+QRIF+PG+ G
Sbjct: 121 LYRSIPYSSFVAFFALYLGVVRNPSFSRYVRFNSMQAVTLDVLLVLPLLVQRIFSPGRSG 180

Query: 181 LGFKIMVMGHNFLFVFIVSCFVYGVVSSVLGKTPYLPLVADAAGRQM 224
           LGF+IMV  H+ LFVF V CFVYG  SSVLG+TPYLP VADAAGRQ+
Sbjct: 181 LGFRIMVWVHSALFVFSVLCFVYGSASSVLGRTPYLPFVADAAGRQI 224

[Close]

BLAST of Spo25805.1 vs. UniProtKB/TrEMBL
Match: A0A022RH21_ERYGU (Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a013812mg PE=4 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 5.900e-58
Identity = 135/224 (60.27%), Postives = 164/224 (73.21%), Query Frame = 1

		  
Query: 1   MASIPLLRLSHLPITTATTTTHHHKYTQIRPLSLLYHPLKSPPTTKTPFQITPKKPKTLK 60
           MASIPLLRLS LP+       HH +++    L LL HPLK    +    +  P+K  T K
Sbjct: 1   MASIPLLRLS-LPL-------HHRQHSGPTAL-LLPHPLKHTLISPPSLRQAPRKTTTTK 60

Query: 61  PNQKPISMSATTPATDRLISSISYFLPFVNGLHYGRFLFAQYPPLSLLFEPILPFLSLYK 120
               P      TPATDRLIS+ +YFLP  NGL YGRFLFAQYP L+  F+P+ P LSLY 
Sbjct: 61  CTYTP------TPATDRLISAAAYFLPLFNGLQYGRFLFAQYPILAAPFQPLFPLLSLYH 120

Query: 121 SIPYASFVSFFGLYLGVVRNPKFSNYVRFNAMQALVLDVLLVLPLLLQRIFTPGK-GLGF 180
           S+P ASFV+FFGLYLGVV+NP  S YVRFNA+QALVLDVLLVLP+L+QRIFTPG+ G+  
Sbjct: 121 SVPSASFVTFFGLYLGVVKNPNLSRYVRFNALQALVLDVLLVLPVLVQRIFTPGRSGIAL 180

Query: 181 KIMVMGHNFLFVFIVSCFVYGVVSSVLGKTPYLPLVADAAGRQM 224
           K+    ++ LF+F+V+CFVYG+VSSVLGKTPYLPLVA+AAGRQ+
Sbjct: 181 KLTAWAYSGLFMFVVACFVYGLVSSVLGKTPYLPLVAEAAGRQL 209

[Close]

BLAST of Spo25805.1 vs. UniProtKB/TrEMBL
Match: A5C9T8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_04s0023g00790 PE=4 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 1.000e-57
Identity = 135/239 (56.49%), Postives = 167/239 (69.87%), Query Frame = 1

		  
Query: 1   MASIPLLRLSHLPITTATTTTHHHKYTQIRPLSLLY-------------HPLKSPPTTKT 60
           MASIPLLRLS             H+ T ++PL   Y             HPLK  PT + 
Sbjct: 1   MASIPLLRLSL------------HRQTPLKPLHQPYSAASITAQLPNFPHPLK--PTLQI 60

Query: 61  PFQITPKKPKTLKPNQKP-ISMS-ATTPATDRLISSISYFLPFVNGLHYGRFLFAQYPPL 120
                   P+ L+P  +P I+MS +  PAT+RLIS+ +Y LPF NGL YGRFL  QYP L
Sbjct: 61  -------SPRPLRPRSRPSITMSYSAVPATERLISAAAYCLPFFNGLQYGRFLLMQYPAL 120

Query: 121 SLLFEPILPFLSLYKSIPYASFVSFFGLYLGVVRNPKFSNYVRFNAMQALVLDVLLVLPL 180
            L+ EPILP LSLY+SIPYASFV+FF LYLGVVRNP FS +VRFN+MQA+VLDVLLV+PL
Sbjct: 121 GLVLEPILPILSLYRSIPYASFVAFFALYLGVVRNPSFSRFVRFNSMQAVVLDVLLVVPL 180

Query: 181 LLQRIFTPGK-GLGFKIMVMGHNFLFVFIVSCFVYGVVSSVLGKTPYLPLVADAAGRQM 224
           L+ RIF PG+ G+GF++MVMGHN +FVF+V+CF+Y +   +LG+TPYLP VADAAGRQ+
Sbjct: 181 LVNRIFNPGRAGVGFRLMVMGHNAIFVFVVACFLYSLGFCILGRTPYLPFVADAAGRQL 218

[Close]

BLAST of Spo25805.1 vs. ExPASy Swiss-Prot
Match: TI202_ARATH (Protein TIC 20-II, chloroplastic OS=Arabidopsis thaliana GN=TIC20-II PE=2 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 2.800e-53
Identity = 123/217 (56.68%), Postives = 149/217 (68.66%), Query Frame = 1

		  
Query: 9   LSHLPITTATTTTHHHKYTQIRPLSLLYHPLKSPPTTKTPFQITPKKPKTLKPNQKPISM 68
           ++ L ++   T T+     + RPLSL +      P + T F I P   +      +  + 
Sbjct: 1   MASLCLSLHQTLTNPLSAPRCRPLSLSF------PGSST-FSIRPSSRRATALTTR--AS 60

Query: 69  SATTPATDRLISSISYFLPFVNGLHYGRFLFAQYPPLSLLFEPILPFLSLYKSIPYASFV 128
              TPAT+R+IS  SY LPF N L YGRFLFAQYP L LLFEPI P L+LY+S+PYASFV
Sbjct: 61  YTPTPATERVISIASYALPFFNSLQYGRFLFAQYPRLGLLFEPIFPILNLYRSVPYASFV 120

Query: 129 SFFGLYLGVVRNPKFSNYVRFNAMQALVLDVLLVLPLLLQRIFTPGKGLGF--KIMVMGH 188
           +FFGLYLGVVRN  FS YVRFNAMQA+ LDVLL +P+LL RI  PG+G GF  K M+ GH
Sbjct: 121 AFFGLYLGVVRNTSFSRYVRFNAMQAVTLDVLLAVPVLLTRILDPGQGGGFGMKAMMWGH 180

Query: 189 NFLFVFIVSCFVYGVVSSVLGKTPYLPLVADAAGRQM 224
             +FVF   CFVYGVVSS+LGKTPY+P VADAAGRQ+
Sbjct: 181 TGVFVFSFMCFVYGVVSSLLGKTPYIPFVADAAGRQL 208

[Close]

BLAST of Spo25805.1 vs. ExPASy Swiss-Prot
Match: TI205_ARATH (Protein TIC 20-v, chloroplastic OS=Arabidopsis thaliana GN=TIC20-V PE=2 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.000e-34
Identity = 71/150 (47.33%), Postives = 102/150 (68.00%), Query Frame = 1

		  
Query: 74  ATDRLISSISYFLPFVNGLHYGRFLFAQYPPLSLLFEPILPFLSLYKSIPYASFVSFFGL 133
           A+DR+IS++ YF PF +G+ YG+F+  QY P  +L +P+ P +  +KS P+  F+ F  L
Sbjct: 59  ASDRIISAVCYFYPFFDGIQYGKFIITQYQPFQILIQPLFPAIRAFKSFPFNGFLIFITL 118

Query: 134 YLGVVRNPKFSNYVRFNAMQALVLDVLLVLPLLLQRIFTPGKGLGFKIMVMGHNFLFVFI 193
           Y  VVRNP FS YVRFN MQA+VLDVLL+ P LL+R F P  G G  +++   + +F+F+
Sbjct: 119 YFVVVRNPNFSRYVRFNTMQAIVLDVLLIFPDLLERSFNPRDGFGLDVVMSLDSTVFLFL 178

Query: 194 VSCFVYGVVSSVLGKTPYLPLVADAAGRQM 224
           +   +YG  + + G TP LPLVA+AA RQ+
Sbjct: 179 LVSLIYGFSACLFGLTPRLPLVAEAADRQV 208

[Close]

BLAST of Spo25805.1 vs. ExPASy Swiss-Prot
Match: YC60L_SYNY3 (Tic20 family protein OS=Synechocystis sp. (strain PCC 6803 / Kazusa) GN=sll1737 PE=3 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 5.200e-23
Identity = 57/159 (35.85%), Postives = 98/159 (61.64%), Query Frame = 1

		  
Query: 66  ISMSATTPATDRLISSISYFLPFVNGLHYGRFLFAQYPPLSLLFEPILPFLSLYKSIPYA 125
           ++ ++T    DR  S++ Y +P ++   +G FL  Q+P L +++ PI+P L  Y   P+A
Sbjct: 1   MASNSTADGKDRFFSALIYVIPLIDAFMFGGFLLQQFPVLQIIYLPIMPLLQFYYQFPFA 60

Query: 126 SFVSFFGLYLGVVRNPKFSNYVRFNAMQALVLDVLLVL-PLLLQRIFTPGKGLGFKIMVM 185
           SF+ F  L++ VVRN   S+++RFNAMQA+++ +LL L  L++  +  P  G G     +
Sbjct: 61  SFIIFIVLFMAVVRNNNISHFIRFNAMQAILIGILLSLFGLIVAYVIQPVFGQGLVTETL 120

Query: 186 GHNFLFVFIVSCFVYGVVSSVLGKTPYLPLVADAAGRQM 224
            +NF F+  ++C  +G+V SVLG+   +P ++DAA  Q+
Sbjct: 121 -YNFAFLGALACGFFGIVQSVLGRYAEIPTISDAAYSQV 158

[Close]

BLAST of Spo25805.1 vs. ExPASy Swiss-Prot
Match: YCF60_PYRYE (Tic20 family protein Ycf60 OS=Pyropia yezoensis GN=ycf60 PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.300e-10
Identity = 55/176 (31.25%), Postives = 95/176 (53.98%), Query Frame = 1

		  
Query: 57  KTLKPNQKPISMSATTPATDRLISSISYFLPFVNGL-HYGRFLFAQYP--PLSLLFEPIL 116
           K +K N+K  +  + T  + RL+S++ Y+LP   GL ++G+++   YP   + L  + IL
Sbjct: 27  KYIKLNKKINNTESKTRLSIRLVSTVPYYLPLFEGLQNFGQYVLPDYPVAAIPLYKKIIL 86

Query: 117 PFLSLYKSIPYASFVSFFGLYLGVVRNPK---FSNYVRFNAMQALVLDVLLVLPLLLQRI 176
           P L  Y +      V+FF LY  +VRN         VRFN+MQ+++L ++  L   + R 
Sbjct: 87  PMLIFYMNHAILGLVTFFALYYVLVRNKSPIPVHQLVRFNSMQSILLFLVGSLFGAVFRA 146

Query: 177 FTPGKGLGFKIMVMGH---NFLFVFIVSCFVYGVVSSVLGKTPYLPLVADAAGRQM 224
           F     + F+I  +G    N +F F++S   Y +V +V GK   +P++++A   Q+
Sbjct: 147 FP----IEFRISFLGLMVCNMMFWFVLSTITYSIVKAVQGKYSNIPVISEAVRIQI 198

[Close]

BLAST of Spo25805.1 vs. ExPASy Swiss-Prot
Match: YCF60_PORPU (Tic20 family protein Ycf60 OS=Porphyra purpurea GN=ycf60 PE=3 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 3.300e-9
Identity = 50/156 (32.05%), Postives = 84/156 (53.85%), Query Frame = 1

		  
Query: 77  RLISSISYFLPFVNGL-HYGRFLFAQYP--PLSLLFEPILPFLSLYKSIPYASFVSFFGL 136
           RL+S I Y+LP   GL ++G+++   YP   + L  + +LP L  Y +      V+FF L
Sbjct: 47  RLVSIIPYYLPLFEGLQNFGQYVLPDYPVGAIPLYKKILLPMLIFYMNHAILGLVTFFAL 106

Query: 137 YLGVVRNPK---FSNYVRFNAMQALVLDVLLVLPLLLQRIFTPGKGLGFKIMVMGH---N 196
           Y  +VRN         VRFN+MQ+++L ++  L   + R F     + F+I  +G    N
Sbjct: 107 YYVLVRNKSPITVHQLVRFNSMQSILLFLVGSLFGAIFRAFP----IEFRISFIGLTVCN 166

Query: 197 FLFVFIVSCFVYGVVSSVLGKTPYLPLVADAAGRQM 224
            +F FI+S   Y +V ++ GK   +P++++A   Q+
Sbjct: 167 MMFWFILSTITYSIVKAIQGKYSNIPVISEAVRIQI 198

[Close]

BLAST of Spo25805.1 vs. TAIR (Arabidopsis)
Match: AT2G47840.1 (Uncharacterised conserved protein ycf60)

HSP 1 Score: 209.9 bits (533), Expect = 1.600e-54
Identity = 123/217 (56.68%), Postives = 149/217 (68.66%), Query Frame = 1

		  
Query: 9   LSHLPITTATTTTHHHKYTQIRPLSLLYHPLKSPPTTKTPFQITPKKPKTLKPNQKPISM 68
           ++ L ++   T T+     + RPLSL +      P + T F I P   +      +  + 
Sbjct: 1   MASLCLSLHQTLTNPLSAPRCRPLSLSF------PGSST-FSIRPSSRRATALTTR--AS 60

Query: 69  SATTPATDRLISSISYFLPFVNGLHYGRFLFAQYPPLSLLFEPILPFLSLYKSIPYASFV 128
              TPAT+R+IS  SY LPF N L YGRFLFAQYP L LLFEPI P L+LY+S+PYASFV
Sbjct: 61  YTPTPATERVISIASYALPFFNSLQYGRFLFAQYPRLGLLFEPIFPILNLYRSVPYASFV 120

Query: 129 SFFGLYLGVVRNPKFSNYVRFNAMQALVLDVLLVLPLLLQRIFTPGKGLGF--KIMVMGH 188
           +FFGLYLGVVRN  FS YVRFNAMQA+ LDVLL +P+LL RI  PG+G GF  K M+ GH
Sbjct: 121 AFFGLYLGVVRNTSFSRYVRFNAMQAVTLDVLLAVPVLLTRILDPGQGGGFGMKAMMWGH 180

Query: 189 NFLFVFIVSCFVYGVVSSVLGKTPYLPLVADAAGRQM 224
             +FVF   CFVYGVVSS+LGKTPY+P VADAAGRQ+
Sbjct: 181 TGVFVFSFMCFVYGVVSSLLGKTPYIPFVADAAGRQL 208

[Close]

BLAST of Spo25805.1 vs. TAIR (Arabidopsis)
Match: AT5G55710.1 (FUNCTIONS IN: molecular_function unknown)

HSP 1 Score: 148.3 bits (373), Expect = 5.700e-36
Identity = 71/150 (47.33%), Postives = 102/150 (68.00%), Query Frame = 1

		  
Query: 74  ATDRLISSISYFLPFVNGLHYGRFLFAQYPPLSLLFEPILPFLSLYKSIPYASFVSFFGL 133
           A+DR+IS++ YF PF +G+ YG+F+  QY P  +L +P+ P +  +KS P+  F+ F  L
Sbjct: 59  ASDRIISAVCYFYPFFDGIQYGKFIITQYQPFQILIQPLFPAIRAFKSFPFNGFLIFITL 118

Query: 134 YLGVVRNPKFSNYVRFNAMQALVLDVLLVLPLLLQRIFTPGKGLGFKIMVMGHNFLFVFI 193
           Y  VVRNP FS YVRFN MQA+VLDVLL+ P LL+R F P  G G  +++   + +F+F+
Sbjct: 119 YFVVVRNPNFSRYVRFNTMQAIVLDVLLIFPDLLERSFNPRDGFGLDVVMSLDSTVFLFL 178

Query: 194 VSCFVYGVVSSVLGKTPYLPLVADAAGRQM 224
           +   +YG  + + G TP LPLVA+AA RQ+
Sbjct: 179 LVSLIYGFSACLFGLTPRLPLVAEAADRQV 208

The following BLAST results are available for this feature:

BLAST of Spo25805.1 vs. NCBI nr
BLAST of Spo25805.1 vs. UniProtKB/TrEMBL
BLAST of Spo25805.1 vs. ExPASy Swiss-Prot
BLAST of Spo25805.1 vs. TAIR (Arabidopsis)

BLAST of Spo25805.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
gi\|731326196\|ref\|XP_010673897.1\|	1.4e-89	81.3	PREDICTED: protein TIC 20-II, ...	[more]
gi\|902229084\|gb\|KNA21374.1\|	1.8e-79	100.	hypothetical protein SOVF_0437...	[more]
gi\|870863562\|gb\|KMT14726.1\|	9.7e-70	86.0	hypothetical protein BVRB_4g07...	[more]
gi\|703116818\|ref\|XP_010101228.1\|	3.4e-59	61.2	hypothetical protein L484_0055...	[more]
gi\|764596660\|ref\|XP_011466077.1\|	2.9e-58	72.0	PREDICTED: protein TIC 20-II, ...	[more]

BLAST of Spo25805.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
A0A0K9RR52_SPIOL	1.2e-79	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8FFX6_BETVU	6.7e-70	86.0	Uncharacterized protein OS=Bet...	[more]
W9RQC5_9ROSA	2.4e-59	61.2	Uncharacterized protein OS=Mor...	[more]
A0A022RH21_ERYGU	5.9e-58	60.2	Uncharacterized protein OS=Ery...	[more]
A5C9T8_VITVI	1.0e-57	56.4	Putative uncharacterized prote...	[more]

BLAST of Spo25805.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
TI202_ARATH	2.8e-53	56.6	Protein TIC 20-II, chloroplast...	[more]
TI205_ARATH	1.0e-34	47.3	Protein TIC 20-v, chloroplasti...	[more]
YC60L_SYNY3	5.2e-23	35.8	Tic20 family protein OS=Synech...	[more]
YCF60_PYRYE	1.3e-10	31.2	Tic20 family protein Ycf60 OS=...	[more]
YCF60_PORPU	3.3e-9	32.0	Tic20 family protein Ycf60 OS=...	[more]

BLAST of Spo25805.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 2

Position :

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x 1


Match Name	E-value	Identity	Description
AT2G47840.1	1.6e-54	56.6	Uncharacterised conserved prot...	[more]
AT5G55710.1	5.7e-36	47.3	FUNCTIONS IN: molecular_functi...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005691	Chloroplast protein import component Tic20	PFAM	PF16166	TIC20	coord: 76..219 score: 8.3
None	No IPR available	PANTHER	PTHR33510	FAMILY NOT NAMED	coord: 1..223 score: 6.7
None	No IPR available	PANTHER	PTHR33510:SF1	PROTEIN TIC 20-II, CHLOROPLASTIC	coord: 1..223 score: 6.7

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010207	photosystem II assembly
biological_process	GO:0032543	mitochondrial translation
biological_process	GO:0010155	regulation of proton transport
biological_process	GO:0006479	protein methylation
biological_process	GO:0046777	protein autophosphorylation
biological_process	GO:0042742	defense response to bacterium
biological_process	GO:0015031	protein transport
biological_process	GO:0006655	phosphatidylglycerol biosynthetic process
biological_process	GO:0070681	glutaminyl-tRNAGln biosynthesis via transamidation
biological_process	GO:0009407	toxin catabolic process
biological_process	GO:0006536	glutamate metabolic process
biological_process	GO:0006979	response to oxidative stress
biological_process	GO:0006418	tRNA aminoacylation for protein translation
biological_process	GO:0009099	valine biosynthetic process
biological_process	GO:0009098	leucine biosynthetic process
biological_process	GO:0009097	isoleucine biosynthetic process
biological_process	GO:0010583	response to cyclopentenone
biological_process	GO:0006189	'de novo' IMP biosynthetic process
biological_process	GO:0010020	chloroplast fission
biological_process	GO:0051607	defense response to virus
biological_process	GO:0009306	protein secretion
biological_process	GO:0016075	rRNA catabolic process
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process	GO:0051252	regulation of RNA metabolic process
biological_process	GO:0010267	production of ta-siRNAs involved in RNA interference
biological_process	GO:0035196	production of miRNAs involved in gene silencing by miRNA
biological_process	GO:0006206	pyrimidine nucleobase metabolic process
biological_process	GO:0006541	glutamine metabolic process
biological_process	GO:0006508	proteolysis
biological_process	GO:0006230	TMP biosynthetic process
biological_process	GO:0010027	thylakoid membrane organization
biological_process	GO:0009793	embryo development ending in seed dormancy
biological_process	GO:0000478	endonucleolytic cleavage involved in rRNA processing
biological_process	GO:0009113	purine nucleobase biosynthetic process
biological_process	GO:0009116	nucleoside metabolic process
biological_process	GO:0000918	division septum site selection
biological_process	GO:0006090	pyruvate metabolic process
biological_process	GO:0006631	fatty acid metabolic process
biological_process	GO:0015995	chlorophyll biosynthetic process
biological_process	GO:0006412	translation
biological_process	GO:0042254	ribosome biogenesis
biological_process	GO:0006099	tricarboxylic acid cycle
biological_process	GO:0006355	regulation of transcription, DNA-templated
biological_process	GO:0019288	isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
biological_process	GO:0006353	DNA-templated transcription, termination
biological_process	GO:0009813	flavonoid biosynthetic process
biological_process	GO:0019344	cysteine biosynthetic process
biological_process	GO:0009073	aromatic amino acid family biosynthetic process
biological_process	GO:0030154	cell differentiation
biological_process	GO:0009965	leaf morphogenesis
biological_process	GO:0019243	methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione
biological_process	GO:0045893	positive regulation of transcription, DNA-templated
biological_process	GO:0006364	rRNA processing
biological_process	GO:0009735	response to cytokinin
biological_process	GO:0009640	photomorphogenesis
biological_process	GO:0006782	protoporphyrinogen IX biosynthetic process
biological_process	GO:0000338	protein deneddylation
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0009220	pyrimidine ribonucleotide biosynthetic process
cellular_component	GO:0005634	nucleus
cellular_component	GO:0005840	ribosome
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0022626	cytosolic ribosome
cellular_component	GO:0009706	chloroplast inner membrane
cellular_component	GO:0009570	chloroplast stroma
cellular_component	GO:0009535	chloroplast thylakoid membrane
cellular_component	GO:0031969	chloroplast membrane
cellular_component	GO:0000311	plastid large ribosomal subunit
cellular_component	GO:0044424	intracellular part
cellular_component	GO:0048046	apoplast
cellular_component	GO:0009579	thylakoid
cellular_component	GO:0009536	plastid
cellular_component	GO:0009505	plant-type cell wall
cellular_component	GO:0009941	chloroplast envelope
cellular_component	GO:0030956	glutamyl-tRNA(Gln) amidotransferase complex
cellular_component	GO:0005654	nucleoplasm
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0005730	nucleolus
cellular_component	GO:0016020	membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0045430	chalcone isomerase activity
molecular_function	GO:0005488	binding
molecular_function	GO:0004449	isocitrate dehydrogenase (NAD+) activity
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0004797	thymidine kinase activity
molecular_function	GO:0005504	fatty acid binding
molecular_function	GO:0051920	peroxiredoxin activity
molecular_function	GO:0004601	peroxidase activity
molecular_function	GO:0008565	protein transporter activity
molecular_function	GO:0050567	glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity
molecular_function	GO:0004525	ribonuclease III activity
molecular_function	GO:0030899	calcium-dependent ATPase activity
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0008266	poly(U) RNA binding
molecular_function	GO:0019843	rRNA binding
molecular_function	GO:0003735	structural constituent of ribosome
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0000166	nucleotide binding
molecular_function	GO:0004109	coproporphyrinogen oxidase activity
molecular_function	GO:0042803	protein homodimerization activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003852	2-isopropylmalate synthase activity
molecular_function	GO:0004252	serine-type endopeptidase activity
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity
molecular_function	GO:0000049	tRNA binding
molecular_function	GO:0016740	transferase activity
molecular_function	GO:0046406	magnesium protoporphyrin IX methyltransferase activity
molecular_function	GO:0008276	protein methyltransferase activity
molecular_function	GO:0005515	protein binding
molecular_function	GO:0004044	amidophosphoribosyltransferase activity
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0070181	small ribosomal subunit rRNA binding
molecular_function	GO:0051287	NAD binding

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo04445	0.79	Barchart \| Table
Spo18209	0.79	Barchart \| Table
Spo21020	0.78	Barchart \| Table
Spo22182	0.76	Barchart \| Table
Spo17491	0.75	Barchart \| Table
Spo00568	0.73	Barchart \| Table
Spo22530	0.73	Barchart \| Table
Spo18908	0.72	Barchart \| Table
Spo26836	0.72	Barchart \| Table
Spo06615	0.71	Barchart \| Table
Spo14353	0.71	Barchart \| Table
Spo07490	0.71	Barchart \| Table
Spo23767	0.70	Barchart \| Table
Spo11597	0.70	Barchart \| Table
Spo09458	0.70	Barchart \| Table
Spo19197	0.69	Barchart \| Table
Spo10961	0.69	Barchart \| Table
Spo16806	0.69	Barchart \| Table
Spo19901	0.69	Barchart \| Table
Spo25370	0.68	Barchart \| Table
Spo11248	0.68	Barchart \| Table
Spo00806	0.68	Barchart \| Table
Spo06411	0.67	Barchart \| Table
Spo10461	0.67	Barchart \| Table
Spo20387	0.67	Barchart \| Table
Spo23512	0.67	Barchart \| Table
Spo11235	0.67	Barchart \| Table
Spo26006	0.67	Barchart \| Table
Spo12865	0.67	Barchart \| Table
Spo25451	0.66	Barchart \| Table
Spo19220	0.66	Barchart \| Table
Spo22941	0.66	Barchart \| Table
Spo05699	0.65	Barchart \| Table
Spo09210	0.65	Barchart \| Table