Spo25880 (gene) | SpinachBase

Overview


Name	Spo25880
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	(2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein) (Hyoscyamine 6-dioxygenase hydroxylase, putative)
Location	chr6 : 41499049 .. 41503447 (-)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ACGTGAAACATGAAAAGTCGTCAGATGACAAAATCGATATTAATTTCTAATGACGTCTAATTATTATATTGACTGTCCTAAAACTAAACTCATAATACAAGAGAACTTTCATAGTGTTGTTATTAGCAAAGGAGGATCGATCGAGGATGGGAGTCGGAGAGGAGACTACTACGACGGTGGCTAACCTTCCCATTATCGACTTATCGGCGCCTGATCGAGAATCCACGGCTGCAACACTTCGCCAGGTAGATTCGATCTATATATATCTGTCTCAAATTAGTTTTTACACTTTTATGATATAATCTCGATCTACTGATATATTTTGCTTCTAACTTAAATTAAATTACTTTTACGTATTAAAAATGTAACACCCTTTTGGTATTTGTGATTATAATATAATATAGGCATGCATTGACTTCGGTTTCTTCTACATTGTGAACCATGGAGTGGACAAGGATCTGTTTGGGAGATTGTTTGAGGAGAGTCAAAAGTTCTTTACACTTCCTTTGGATGATAAAATGAAAATAGCTCGCAAAGGAATTCGTGGATATACTCCTTTATATCACGAGAAGTTGGATACCGCTACCTCTACCAAAGGTTTTTCTACCTCTAAAACTATATAGCTCTTTTTACCGCTATTTTTTACTCCTTCCGTCCCGGAATATTTGAACCGATTTAACCGACACAGAGTTTAGTACAAAGTAATTGGCTTATTATTTAATGAGATTGTAGTTGATAGTGTTGTATTTTTTAATATAGATAGGAATATGTGACAACTTTTTAAAGGGGTTAAAGAGTAGGGGGAGGGTGCATGAGACCACAAAATGACATGTGAAAGAGGTAGTTGATATAATATTTATAAAAATTTACCATTTATAGAAGTAATCTGAAAAGGCTTTATAAGGAAAACTGTGCAAGTAGTCCGGGACGGAGGGAATATTTATTTATTGGTATAAAAGTTTTACGTGGTAGATAAACTTGGAATCGAATCGTGTACATGTTACTGTGAAAGTGTGAATAACAATTGTGCTGCAAATTTGAAGGGAGTTTTTTGATGTTAGGTGATTCTAAGGAGTTTTTGGACATTGGGCCGCTTCATGATGAAGTTAGAGATTCGAACCAGTGGCCTTCTGAAGGTAAGAAAACAAAGACATAAAGGAAGGAATAGTCTCCTAAATAAGACGCGCTGACGTGGTAAAGCCACCCCTCTAATTTCCTCATCAAAATATATATGTACAAACAAATTAAGCCAAATGGAATAATATTTAAAATGGTGAGGGACTACTCTAGAATCTAGATTCATCTAAAAGTCAAGTTGATTTTGGCATATAGTATGGACAATCTGTCCATCTCTGCCTCAATTTAGTTGCGACAAAATTTTTGTACGATAATGGTCCACATAAACTTAATTATGTGTAAGATTAGGGTGTTTATCTTTATCTAAGGGGTGCCTATACTTTTTTTGCATAATTTTTTATTGTTCTGCGAGCAATAAAAAAAGAGTGAAAATTAGTTTAAATAACAATATCAATGTGACAACTAATGTTAAAGCAAAAATAAACTATTATAGGATAAAACCTTAAGTATATAATTCTTAGTTTCAAAGTTAAAAAAATGTTAAAGCAAAAATCGGGAACAATATCAACGTGACAACTAATGTTAAAGCAAAAATAGTGAAAATTAGTTAAAAATAACAATATCAACGTGACAACTAATGTTAAAGCATACATCCAATGTACTTTAGGATTTAATATAAGAATTTGATTAGAATTCGGATACTAATTAATTTCTTTAATCATCTACTGATGATTGTGGCATTCCAGAGCTTCTTCCAAACTGGAAAACAACCGTGGAAGCATACCATGATAAAATCATGTAAGTTATGAAGTACTAAATTTTCATTGCCTTATTTGTTCGCTTTCCTTGCATATCAATATTAGTGGTCGCTTTGTCTAATTACCGCTTACTACCTCCGTTGTATAATGTTTTTTACGGTTATTAACTTATTATTTGCACAAATATTAAGGAAAAAAGAGAAGCTATAGTGTTTAAAAATGTGGGTGAATGAAATAAAATATGAATAAAATAAGTGAGGGAGAGTATAAGGTGGGTGGAGAAAAAATGAATAAAATTAGAGAAAGTGAGTGAAAAGTGATAAATGTTGTGGAGGTAATAGGGGTAAGATAGTAAAGTTTCATGTCAAGAAATAGAATAAAACACAATGTAAGAACAATAAGAATAGATTAAAACAGAAAGCGCGCGTAAAGATCATTTTAAAACGGAGGTAGTATTTGTTAAGATAATTTTGGTGTTAGGCAAGGGCAGATTCTGAACAGTTTTACGAGGGGGGTCGGATTGGCCGGTAGAAAAAAAATTAAGCAATATACTATGTAATAATACAACTATATACAAATTTAGGGGGCTGTAACTTAAAATACACTATAAAATTTTCCGGCCGAGGGAGGGGGGAGAAGTCCCACCACAACACCAACTAAGATCTGCCATTGCCCATTGGTGCTAGGGTGTGTTCTATTTAGCTGATTTTCACTTATTTTCTGAACTTGTCAAAATTTATTTTAGTTATAAATTGTACTCCTTAATAACTTGGTCAACTTTTATTTCTCCTAAACTTATTTTATCTGATTAAACTTATTTTTCCCGAGATAAGTAAAAATAGGGTGAACATAAGAAAACCTTGGTCATATCGTGATATTTAGTTTGTTTTAAATACTCTCAATGTAAGTATGTAACACAAAGTGTGGTGCCTTAATTAGGAAAGCTGGAAAAAGATTATATTCACTTCTTGCTGTGGCCTTAAACCTAAGTGAGAACTATTTCGAAGACATTGGAGCTTTGGATCCACCAATGCCTTTCATTCGCCTCTTACGTTACCCAGGTTAGCTAGCTGTAACGAAACATGATACTACGTATATAATTATATATGTATAGGGGACTTGAGCTGTTATATTTTGGATTTGCTTAATTGGCTGTAATTAGTGTTTGATTAAACATGTTTAGGGGACCTCAGTTACTACACTAATGAAGTTCTAGGTGCTTCTGCTCATTGTGATTATGGAATGATGACCCTATTAGCGACTGATGGTGTTGCTGGACTACAGGTACACCTATGCATATATATGTTACAATTAATATGTTAGTTACATTTCCTAATTGTTATTGTAAACTGTAAGGTTACATAATTAATCTACCATGTTTCCTCAGGTTTGCCGGGAAAAAGATCGTCAGCCACGTATTTGGGAGGATGTTCTTCATATTGAGGGGTGAGTCATCCTACATATATTAGATCTGCCTGATCATGAAGCTTTTCAAATTAAGCCTGAACTATGGACACATGTCCATGATGATCTAAATTGCGCTCTTGTACGTGATTATTGACAAGTTTGTTACATTTGCAGGGCCTTAGTTGTCAACATTGGGGACCTTACTGAGAGGTGGACGAATAACGTGTTTAGGTAAATGTTTAATTAATTAACGGGCATTCTACTTATTGTCAGTTATGAAACCCTAATTTTGAATGACAATGATGTACTGTATCAAGAACTACGGATCGAATCAATCGTTTTACATCATTACCGTTTGTAACTCAGTACCCTAATTGCTTTAATATAAACATGCATGTCAAAAGATATCGTGTTTTTCGCCTTAAATAAGTCTGCTGGTTTATGACAGGTCAACATTGCATAGAGTGGTGCCAACAGGACAAGAACGTTACTCAGTACGTAATTTATAGTTTTATTCCTTTTGCTTCTCATATATCCTTCGTTTCTTTTGTTGTTCAACTTACTATATATACTTCGTCGATCTATTTGTTCCCTTTATCTCTAAAGAATTTGTTACTTTGTGTATTGATTTACTAGTTGAATGATGAAGTGATAGAACTATTAAATTGTCCTGCAGGTGGCTTTCTATCTTGACCCATCGCATGATCTTATGGTGGAGTGCTTAGAAACTTGTTGCAGCGAATCAAATCCTCCAAGGTGATCTTTTTTAAGTGCACGTACCCTCTCACATTTTCCCCTCACGTTATAGGTTCTACTTTAAATGTGTATTATCTAATCAAGACCTCTTCTCTGTTCGATTGCAGATTTCCTCCATTCCGTTATATTGACTACCTTTTAGAACGTTTTGAGGGCACATTTGAAGCTTGAATTCAAGTTATATATACGGATTAGTAAATAAGTATGAAGTATCATGTAAACTTGTAAAGCCCTATAATTTAAAAGGCTGAATTATATTATGTTAAGGGCTTTGGTATAAGGTTATGTATAGACTTACCCTTGTCAGCTTTGATGGTAAGGGCAGCTTCATCTCTTGTTAATTCGTCTTATGTTAAATACTTTATTCGTTCCTTAAATATTGCACCATTTTAACTTTTATA

mRNA sequence

ACGTGAAACATGAAAAGTCGTCAGATGACAAAATCGATATTAATTTCTAATGACGTCTAATTATTATATTGACTGTCCTAAAACTAAACTCATAATACAAGAGAACTTTCATAGTGTTGTTATTAGCAAAGGAGGATCGATCGAGGATGGGAGTCGGAGAGGAGACTACTACGACGGTGGCTAACCTTCCCATTATCGACTTATCGGCGCCTGATCGAGAATCCACGGCTGCAACACTTCGCCAGGCATGCATTGACTTCGGTTTCTTCTACATTGTGAACCATGGAGTGGACAAGGATCTGTTTGGGAGATTGTTTGAGGAGAGTCAAAAGTTCTTTACACTTCCTTTGGATGATAAAATGAAAATAGCTCGCAAAGGAATTCGTGGATATACTCCTTTATATCACGAGAAGTTGGATACCGCTACCTCTACCAAAGGTGATTCTAAGGAGTTTTTGGACATTGGGCCGCTTCATGATGAAGTTAGAGATTCGAACCAGTGGCCTTCTGAAGAGCTTCTTCCAAACTGGAAAACAACCGTGGAAGCATACCATGATAAAATCATGAAAGCTGGAAAAAGATTATATTCACTTCTTGCTGTGGCCTTAAACCTAAGTGAGAACTATTTCGAAGACATTGGAGCTTTGGATCCACCAATGCCTTTCATTCGCCTCTTACGTTACCCAGGGGACCTCAGTTACTACACTAATGAAGTTCTAGGTGCTTCTGCTCATTGTGATTATGGAATGATGACCCTATTAGCGACTGATGGTGTTGCTGGACTACAGGTTTGCCGGGAAAAAGATCGTCAGCCACGTATTTGGGAGGATGTTCTTCATATTGAGGGGGCCTTAGTTGTCAACATTGGGGACCTTACTGAGAGGTGGACGAATAACGTGTTTAGGTCAACATTGCATAGAGTGGTGCCAACAGGACAAGAACGTTACTCAGTGGCTTTCTATCTTGACCCATCGCATGATCTTATGGTGGAGTGCTTAGAAACTTGTTGCAGCGAATCAAATCCTCCAAGATTTCCTCCATTCCGTTATATTGACTACCTTTTAGAACGTTTTGAGGGCACATTTGAAGCTTGAATTCAAGTTATATATACGGATTAGTAAATAAGTATGAAGTATCATGTAAACTTGTAAAGCCCTATAATTTAAAAGGCTGAATTATATTATGTTAAGGGCTTTGGTATAAGGTTATGTATAGACTTACCCTTGTCAGCTTTGATGGTAAGGGCAGCTTCATCTCTTGTTAATTCGTCTTATGTTAAATACTTTATTCGTTCCTTAAATATTGCACCATTTTAACTTTTATA

Coding sequence (CDS)

ATGGGAGTCGGAGAGGAGACTACTACGACGGTGGCTAACCTTCCCATTATCGACTTATCGGCGCCTGATCGAGAATCCACGGCTGCAACACTTCGCCAGGCATGCATTGACTTCGGTTTCTTCTACATTGTGAACCATGGAGTGGACAAGGATCTGTTTGGGAGATTGTTTGAGGAGAGTCAAAAGTTCTTTACACTTCCTTTGGATGATAAAATGAAAATAGCTCGCAAAGGAATTCGTGGATATACTCCTTTATATCACGAGAAGTTGGATACCGCTACCTCTACCAAAGGTGATTCTAAGGAGTTTTTGGACATTGGGCCGCTTCATGATGAAGTTAGAGATTCGAACCAGTGGCCTTCTGAAGAGCTTCTTCCAAACTGGAAAACAACCGTGGAAGCATACCATGATAAAATCATGAAAGCTGGAAAAAGATTATATTCACTTCTTGCTGTGGCCTTAAACCTAAGTGAGAACTATTTCGAAGACATTGGAGCTTTGGATCCACCAATGCCTTTCATTCGCCTCTTACGTTACCCAGGGGACCTCAGTTACTACACTAATGAAGTTCTAGGTGCTTCTGCTCATTGTGATTATGGAATGATGACCCTATTAGCGACTGATGGTGTTGCTGGACTACAGGTTTGCCGGGAAAAAGATCGTCAGCCACGTATTTGGGAGGATGTTCTTCATATTGAGGGGGCCTTAGTTGTCAACATTGGGGACCTTACTGAGAGGTGGACGAATAACGTGTTTAGGTCAACATTGCATAGAGTGGTGCCAACAGGACAAGAACGTTACTCAGTGGCTTTCTATCTTGACCCATCGCATGATCTTATGGTGGAGTGCTTAGAAACTTGTTGCAGCGAATCAAATCCTCCAAGATTTCCTCCATTCCGTTATATTGACTACCTTTTAGAACGTTTTGAGGGCACATTTGAAGCTTGA

Protein sequence

MGVGEETTTTVANLPIIDLSAPDRESTAATLRQACIDFGFFYIVNHGVDKDLFGRLFEESQKFFTLPLDDKMKIARKGIRGYTPLYHEKLDTATSTKGDSKEFLDIGPLHDEVRDSNQWPSEELLPNWKTTVEAYHDKIMKAGKRLYSLLAVALNLSENYFEDIGALDPPMPFIRLLRYPGDLSYYTNEVLGASAHCDYGMMTLLATDGVAGLQVCREKDRQPRIWEDVLHIEGALVVNIGDLTERWTNNVFRSTLHRVVPTGQERYSVAFYLDPSHDLMVECLETCCSESNPPRFPPFRYIDYLLERFEGTFEA

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo25880.1	Spo25880.1	mRNA

Homology

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BLAST of Spo25880.1 vs. NCBI nr
Match: gi|902237595|gb|KNA24851.1| (hypothetical protein SOVF_011890 [Spinacia oleracea])

HSP 1 Score: 486.1 bits (1250), Expect = 4.600e-134
Identity = 227/312 (72.76%), Postives = 263/312 (84.29%), Query Frame = 1

		  
Query: 5   EETTTTVANLPIIDLSAPDRESTAATLRQACIDFGFFYIVNHGVDKDLFGRLFEESQKFF 64
           E+  + + NLPIIDL APDR STAATLRQACI+ GFFYIVNHGVDKDL GRLFEES+KFF
Sbjct: 4   EDEDSLIVNLPIIDLLAPDRVSTAATLRQACIEVGFFYIVNHGVDKDLLGRLFEESKKFF 63

Query: 65  TLPLDDKMKIARKGIRGYTPLYHEKLDTATSTKGDSKEFLDIGPLHDEVRDSNQWPSEEL 124
            LP+++KMK+ RK IRGYTPLY EKLDT+ +TKGDSKE   IGPLHDE+ + NQWPSEEL
Sbjct: 64  DLPVEEKMKLDRKEIRGYTPLYSEKLDTSLNTKGDSKESFYIGPLHDELCNLNQWPSEEL 123

Query: 125 LPNWKTTVEAYHDKIMKAGKRLYSLLAVALNLSENYFEDIGALDPPMPFIRLLRYP-GDL 184
           LPNW+ T+EAYH +I+ AGK L SLLA ALNL+EN+FE++GALD PMP +RLL YP GD 
Sbjct: 124 LPNWRATMEAYHKQILNAGKSLLSLLAPALNLNENFFENVGALDQPMPVLRLLHYPEGDN 183

Query: 185 SYYTNEVLGASAHCDYGMMTLLATDGVAGLQVCREKDRQPRIWEDVLHIEGALVVNIGDL 244
           +   +EVLGASAH DYGM+TLL TDGV GLQVCREKDRQPRIWEDV H+EGA++VNIGD+
Sbjct: 184 NCNNDEVLGASAHSDYGMITLLVTDGVPGLQVCREKDRQPRIWEDVPHVEGAIIVNIGDI 243

Query: 245 TERWTNNVFRSTLHRVVPTGQERYSVAFYLDPSHDLMVECLETCCSESNPPRFPPFRYID 304
           TERWTN +FRSTLHRVVP G+ER+S A +LDP H+  VECLETCCSESNPPRFPP RY D
Sbjct: 244 TERWTNCLFRSTLHRVVPKGRERFSAALFLDPPHNFSVECLETCCSESNPPRFPPIRYND 303

Query: 305 YLLERFEGTFEA 316
           YL ER+  T+ A
Sbjct: 304 YLKERYRVTYGA 315

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BLAST of Spo25880.1 vs. NCBI nr
Match: gi|731332211|ref|XP_010677081.1| (PREDICTED: flavonol synthase/flavanone 3-hydroxylase-like isoform X1 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 485.3 bits (1248), Expect = 7.900e-134
Identity = 224/310 (72.26%), Postives = 262/310 (84.52%), Query Frame = 1

		  
Query: 6   ETTTTVANLPIIDLSAPDRESTAATLRQACIDFGFFYIVNHGVDKDLFGRLFEESQKFFT 65
           E  + + NLPIIDLSAPDR +TAATLRQACI  GFFYIVNHGV+KDL  RLFEES+KFF+
Sbjct: 2   EEESGIINLPIIDLSAPDRLTTAATLRQACIGVGFFYIVNHGVEKDLLNRLFEESKKFFS 61

Query: 66  LPLDDKMKIARKGIRGYTPLYHEKLDTATSTKGDSKEFLDIGPLHDEVRDSNQWPSEELL 125
           LPL++KM++ RK  RGYT LY EKLD + STKGDSKE   IGPLHDEV + NQWPSEELL
Sbjct: 62  LPLEEKMRLNRKEYRGYTALYSEKLDPSLSTKGDSKESFYIGPLHDEVCNLNQWPSEELL 121

Query: 126 PNWKTTVEAYHDKIMKAGKRLYSLLAVALNLSENYFEDIGALDPPMPFIRLLRYPGDLSY 185
           P+W+  +E YH +++  GKRL SLLA+ALNL E +FE++GALD PM F+R + YPGD+SY
Sbjct: 122 PSWRPIMEEYHRQLLSVGKRLLSLLALALNLDEKFFENVGALDSPMAFLRTIHYPGDISY 181

Query: 186 YTNEVLGASAHCDYGMMTLLATDGVAGLQVCREKDRQPRIWEDVLHIEGALVVNIGDLTE 245
           + +EVLGASAH DYGM+TLLATDGV GLQVCREKDRQPRIWED+L IEGA++VNIGD+TE
Sbjct: 182 HNDEVLGASAHSDYGMITLLATDGVPGLQVCREKDRQPRIWEDILDIEGAIIVNIGDMTE 241

Query: 246 RWTNNVFRSTLHRVVPTGQERYSVAFYLDPSHDLMVECLETCCSESNPPRFPPFRYIDYL 305
           RWTN +FRSTLHRVVP GQERYSVAF+LDP HD +VECLET C+ESNPPRFPP R+IDYL
Sbjct: 242 RWTNCLFRSTLHRVVPKGQERYSVAFFLDPPHDFLVECLETACTESNPPRFPPIRFIDYL 301

Query: 306 LERFEGTFEA 316
            ER+  TF A
Sbjct: 302 QERYRLTFGA 311

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BLAST of Spo25880.1 vs. NCBI nr
Match: gi|731332215|ref|XP_010677083.1| (PREDICTED: probable iron/ascorbate oxidoreductase DDB_G0283291 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 465.7 bits (1197), Expect = 6.400e-128
Identity = 216/313 (69.01%), Postives = 257/313 (82.11%), Query Frame = 1

		  
Query: 3   VGEETTTTVANLPIIDLSAPDRESTAATLRQACIDFGFFYIVNHGVDKDLFGRLFEESQK 62
           +GEE+   + NLP+IDL+APDR  TAATLRQACI+ GFFYIVNHGVDKDL  R+FEES K
Sbjct: 1   MGEESA--IVNLPVIDLAAPDRLVTAATLRQACIEVGFFYIVNHGVDKDLLNRVFEESNK 60

Query: 63  FFTLPLDDKMKIARKGIRGYTPLYHEKLDTATSTKGDSKEFLDIGPLHDEVRDSNQWPSE 122
           FF++PL++K ++  K  RGYT LY E +D + STKGDSKE   IGPLH+EV + NQWPSE
Sbjct: 61  FFSVPLEEKTRLNLKEFRGYTALYSENVDPS-STKGDSKESFYIGPLHNEVCNLNQWPSE 120

Query: 123 ELLPNWKTTVEAYHDKIMKAGKRLYSLLAVALNLSENYFEDIGALDPPMPFIRLLRYPGD 182
           ELLP+W+ T+EAYH +I+ AGK L SLLA+ LNL EN+F+ +GA+DP MPF+RL+ YPGD
Sbjct: 121 ELLPSWRPTIEAYHRQILSAGKNLLSLLALTLNLDENFFDKVGAVDPLMPFLRLIHYPGD 180

Query: 183 LSYYTNEVLGASAHCDYGMMTLLATDGVAGLQVCREKDRQPRIWEDVLHIEGALVVNIGD 242
           L     EV+GA AH DYGM+TLLATDGV GLQVCREKDRQPRIWEDV HIEGA++VNIGD
Sbjct: 181 LRCDNEEVVGAGAHSDYGMITLLATDGVPGLQVCREKDRQPRIWEDVPHIEGAIIVNIGD 240

Query: 243 LTERWTNNVFRSTLHRVVPTGQERYSVAFYLDPSHDLMVECLETCCSESNPPRFPPFRYI 302
           +TERWTN +FRSTLHRVVPTGQER+SVA +LDP H+  VECL+TCCSESNPPRFP  RYI
Sbjct: 241 MTERWTNRLFRSTLHRVVPTGQERFSVACFLDPPHNFSVECLDTCCSESNPPRFPGIRYI 300

Query: 303 DYLLERFEGTFEA 316
           DY+ E +   FEA
Sbjct: 301 DYIQESYRNIFEA 310

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BLAST of Spo25880.1 vs. NCBI nr
Match: gi|694433837|ref|XP_009344185.1| (PREDICTED: 1-aminocyclopropane-1-carboxylate oxidase-like [Pyrus x bretschneideri])

HSP 1 Score: 428.7 bits (1101), Expect = 8.700e-117
Identity = 192/307 (62.54%), Postives = 249/307 (81.11%), Query Frame = 1

		  
Query: 8   TTTVANLPIIDLSAPDRESTAATLRQACIDFGFFYIVNHGVDKDLFGRLFEESQKFFTLP 67
           TT   NLPIIDLS+ D  S+A ++RQACID+GFFY+VNHGV+++LF  +F+ S  FF+LP
Sbjct: 2   TTAAVNLPIIDLSSSDSISSANSIRQACIDYGFFYLVNHGVEEELFAEVFDRSSNFFSLP 61

Query: 68  LDDKMKIARKGIRGYTPLYHEKLDTATSTKGDSKEFLDIGPLHDEVRDS-NQWPSEELLP 127
           L+DK+K+ RK  RGYTPL+ E LD A+S+KGDSKE   +GPL D  + + NQWP E++LP
Sbjct: 62  LEDKLKLTRKHHRGYTPLFSENLDFASSSKGDSKESFYVGPLDDPTQSNLNQWPPEDILP 121

Query: 128 NWKTTVEAYHDKIMKAGKRLYSLLAVALNLSENYFEDIGALDPPMPFIRLLRYPGDLSYY 187
           +W+ T+E+Y+ K+M  GKRL SL+A+ALNL E++FE +GA+D P  F+RLL YPG+L   
Sbjct: 122 SWRPTMESYYRKVMSTGKRLISLIALALNLDEDFFEKVGAMDNPRGFLRLLHYPGELGST 181

Query: 188 TNEVLGASAHCDYGMMTLLATDGVAGLQVCREKDRQPRIWEDVLHIEGALVVNIGDLTER 247
             E+ GASAH DYGM+TLLA++GV GLQVCR+K +QPRIWEDVLHI+GA +VNIGD+ ER
Sbjct: 182 DEEIYGASAHSDYGMVTLLASNGVPGLQVCRDKSKQPRIWEDVLHIDGAFIVNIGDMMER 241

Query: 248 WTNNVFRSTLHRVVPTGQERYSVAFYLDPSHDLMVECLETCCSESNPPRFPPFRYIDYLL 307
           WTN +FRSTLHRV+PTGQERYSVAF++DP+ D +VECL++CCSE++PPRFPP R  DYL 
Sbjct: 242 WTNCLFRSTLHRVMPTGQERYSVAFFMDPNADCVVECLKSCCSEASPPRFPPVRSGDYLE 301

Query: 308 ERFEGTF 314
           ERF  T+
Sbjct: 302 ERFRLTY 308

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BLAST of Spo25880.1 vs. NCBI nr
Match: gi|595847723|ref|XP_007209384.1| (hypothetical protein PRUPE_ppa009079mg [Prunus persica])

HSP 1 Score: 426.8 bits (1096), Expect = 3.300e-116
Identity = 194/305 (63.61%), Postives = 246/305 (80.66%), Query Frame = 1

		  
Query: 9   TTVANLPIIDLSAPDRESTAATLRQACIDFGFFYIVNHGVDKDLFGRLFEESQKFFTLPL 68
           T   NLPIIDLS+PD  S+A ++RQACI+ GFFY+VNHGV+++L G +FEES K F+LPL
Sbjct: 2   TAALNLPIIDLSSPDSLSSATSIRQACIECGFFYLVNHGVEEELLGHVFEESSKLFSLPL 61

Query: 69  DDKMKIARKGIRGYTPLYHEKLDTATSTKGDSKEFLDIGPLHDEVRDSNQWPSEELLPNW 128
           +DK+K+ARK  RGYTPL+ E LD  +++KGDSKE   IGPL D   + NQWPSE++LP+W
Sbjct: 62  EDKLKLARKQHRGYTPLFSENLDPTSTSKGDSKESFYIGPL-DTRSNLNQWPSEDILPSW 121

Query: 129 KTTVEAYHDKIMKAGKRLYSLLAVALNLSENYFEDIGALDPPMPFIRLLRYPGDLSYYTN 188
           + T+E+Y+ K++ AGKRL SL+A+ALNL E +FE +GAL+ P   +RLL YPG L     
Sbjct: 122 RPTMESYYRKVLSAGKRLISLIALALNLDEEFFEKVGALNKPTAVLRLLHYPGQLGSTDE 181

Query: 189 EVLGASAHCDYGMMTLLATDGVAGLQVCREKDRQPRIWEDVLHIEGALVVNIGDLTERWT 248
           E+ GASAH DYGM+TLLA++GV GLQVCREK +QPRIWEDVLHI+GA +VNIGD+ ERWT
Sbjct: 182 EIYGASAHSDYGMVTLLASNGVPGLQVCREKSKQPRIWEDVLHIDGAFIVNIGDMLERWT 241

Query: 249 NNVFRSTLHRVVPTGQERYSVAFYLDPSHDLMVECLETCCSESNPPRFPPFRYIDYLLER 308
           N +FRSTLHRV+P GQERYSVAF+LDP+ D +VECL++CCSE++PPRFPP R  DYL ER
Sbjct: 242 NCLFRSTLHRVMPAGQERYSVAFFLDPNEDFVVECLKSCCSEASPPRFPPIRSGDYLKER 301

Query: 309 FEGTF 314
           F  T+
Sbjct: 302 FRLTY 305

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BLAST of Spo25880.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RZB1_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_011890 PE=3 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 3.200e-134
Identity = 227/312 (72.76%), Postives = 263/312 (84.29%), Query Frame = 1

		  
Query: 5   EETTTTVANLPIIDLSAPDRESTAATLRQACIDFGFFYIVNHGVDKDLFGRLFEESQKFF 64
           E+  + + NLPIIDL APDR STAATLRQACI+ GFFYIVNHGVDKDL GRLFEES+KFF
Sbjct: 4   EDEDSLIVNLPIIDLLAPDRVSTAATLRQACIEVGFFYIVNHGVDKDLLGRLFEESKKFF 63

Query: 65  TLPLDDKMKIARKGIRGYTPLYHEKLDTATSTKGDSKEFLDIGPLHDEVRDSNQWPSEEL 124
            LP+++KMK+ RK IRGYTPLY EKLDT+ +TKGDSKE   IGPLHDE+ + NQWPSEEL
Sbjct: 64  DLPVEEKMKLDRKEIRGYTPLYSEKLDTSLNTKGDSKESFYIGPLHDELCNLNQWPSEEL 123

Query: 125 LPNWKTTVEAYHDKIMKAGKRLYSLLAVALNLSENYFEDIGALDPPMPFIRLLRYP-GDL 184
           LPNW+ T+EAYH +I+ AGK L SLLA ALNL+EN+FE++GALD PMP +RLL YP GD 
Sbjct: 124 LPNWRATMEAYHKQILNAGKSLLSLLAPALNLNENFFENVGALDQPMPVLRLLHYPEGDN 183

Query: 185 SYYTNEVLGASAHCDYGMMTLLATDGVAGLQVCREKDRQPRIWEDVLHIEGALVVNIGDL 244
           +   +EVLGASAH DYGM+TLL TDGV GLQVCREKDRQPRIWEDV H+EGA++VNIGD+
Sbjct: 184 NCNNDEVLGASAHSDYGMITLLVTDGVPGLQVCREKDRQPRIWEDVPHVEGAIIVNIGDI 243

Query: 245 TERWTNNVFRSTLHRVVPTGQERYSVAFYLDPSHDLMVECLETCCSESNPPRFPPFRYID 304
           TERWTN +FRSTLHRVVP G+ER+S A +LDP H+  VECLETCCSESNPPRFPP RY D
Sbjct: 244 TERWTNCLFRSTLHRVVPKGRERFSAALFLDPPHNFSVECLETCCSESNPPRFPPIRYND 303

Query: 305 YLLERFEGTFEA 316
           YL ER+  T+ A
Sbjct: 304 YLKERYRVTYGA 315

[Close]

BLAST of Spo25880.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CD92_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_5g104690 PE=3 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 5.500e-134
Identity = 224/310 (72.26%), Postives = 262/310 (84.52%), Query Frame = 1

		  
Query: 6   ETTTTVANLPIIDLSAPDRESTAATLRQACIDFGFFYIVNHGVDKDLFGRLFEESQKFFT 65
           E  + + NLPIIDLSAPDR +TAATLRQACI  GFFYIVNHGV+KDL  RLFEES+KFF+
Sbjct: 2   EEESGIINLPIIDLSAPDRLTTAATLRQACIGVGFFYIVNHGVEKDLLNRLFEESKKFFS 61

Query: 66  LPLDDKMKIARKGIRGYTPLYHEKLDTATSTKGDSKEFLDIGPLHDEVRDSNQWPSEELL 125
           LPL++KM++ RK  RGYT LY EKLD + STKGDSKE   IGPLHDEV + NQWPSEELL
Sbjct: 62  LPLEEKMRLNRKEYRGYTALYSEKLDPSLSTKGDSKESFYIGPLHDEVCNLNQWPSEELL 121

Query: 126 PNWKTTVEAYHDKIMKAGKRLYSLLAVALNLSENYFEDIGALDPPMPFIRLLRYPGDLSY 185
           P+W+  +E YH +++  GKRL SLLA+ALNL E +FE++GALD PM F+R + YPGD+SY
Sbjct: 122 PSWRPIMEEYHRQLLSVGKRLLSLLALALNLDEKFFENVGALDSPMAFLRTIHYPGDISY 181

Query: 186 YTNEVLGASAHCDYGMMTLLATDGVAGLQVCREKDRQPRIWEDVLHIEGALVVNIGDLTE 245
           + +EVLGASAH DYGM+TLLATDGV GLQVCREKDRQPRIWED+L IEGA++VNIGD+TE
Sbjct: 182 HNDEVLGASAHSDYGMITLLATDGVPGLQVCREKDRQPRIWEDILDIEGAIIVNIGDMTE 241

Query: 246 RWTNNVFRSTLHRVVPTGQERYSVAFYLDPSHDLMVECLETCCSESNPPRFPPFRYIDYL 305
           RWTN +FRSTLHRVVP GQERYSVAF+LDP HD +VECLET C+ESNPPRFPP R+IDYL
Sbjct: 242 RWTNCLFRSTLHRVVPKGQERYSVAFFLDPPHDFLVECLETACTESNPPRFPPIRFIDYL 301

Query: 306 LERFEGTFEA 316
            ER+  TF A
Sbjct: 302 QERYRLTFGA 311

[Close]

BLAST of Spo25880.1 vs. UniProtKB/TrEMBL
Match: A0A0J8F728_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_5g104700 PE=3 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 4.500e-128
Identity = 216/313 (69.01%), Postives = 257/313 (82.11%), Query Frame = 1

		  
Query: 3   VGEETTTTVANLPIIDLSAPDRESTAATLRQACIDFGFFYIVNHGVDKDLFGRLFEESQK 62
           +GEE+   + NLP+IDL+APDR  TAATLRQACI+ GFFYIVNHGVDKDL  R+FEES K
Sbjct: 1   MGEESA--IVNLPVIDLAAPDRLVTAATLRQACIEVGFFYIVNHGVDKDLLNRVFEESNK 60

Query: 63  FFTLPLDDKMKIARKGIRGYTPLYHEKLDTATSTKGDSKEFLDIGPLHDEVRDSNQWPSE 122
           FF++PL++K ++  K  RGYT LY E +D + STKGDSKE   IGPLH+EV + NQWPSE
Sbjct: 61  FFSVPLEEKTRLNLKEFRGYTALYSENVDPS-STKGDSKESFYIGPLHNEVCNLNQWPSE 120

Query: 123 ELLPNWKTTVEAYHDKIMKAGKRLYSLLAVALNLSENYFEDIGALDPPMPFIRLLRYPGD 182
           ELLP+W+ T+EAYH +I+ AGK L SLLA+ LNL EN+F+ +GA+DP MPF+RL+ YPGD
Sbjct: 121 ELLPSWRPTIEAYHRQILSAGKNLLSLLALTLNLDENFFDKVGAVDPLMPFLRLIHYPGD 180

Query: 183 LSYYTNEVLGASAHCDYGMMTLLATDGVAGLQVCREKDRQPRIWEDVLHIEGALVVNIGD 242
           L     EV+GA AH DYGM+TLLATDGV GLQVCREKDRQPRIWEDV HIEGA++VNIGD
Sbjct: 181 LRCDNEEVVGAGAHSDYGMITLLATDGVPGLQVCREKDRQPRIWEDVPHIEGAIIVNIGD 240

Query: 243 LTERWTNNVFRSTLHRVVPTGQERYSVAFYLDPSHDLMVECLETCCSESNPPRFPPFRYI 302
           +TERWTN +FRSTLHRVVPTGQER+SVA +LDP H+  VECL+TCCSESNPPRFP  RYI
Sbjct: 241 MTERWTNRLFRSTLHRVVPTGQERFSVACFLDPPHNFSVECLDTCCSESNPPRFPGIRYI 300

Query: 303 DYLLERFEGTFEA 316
           DY+ E +   FEA
Sbjct: 301 DYIQESYRNIFEA 310

[Close]

BLAST of Spo25880.1 vs. UniProtKB/TrEMBL
Match: M5WMS3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009079mg PE=3 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 2.300e-116
Identity = 194/305 (63.61%), Postives = 246/305 (80.66%), Query Frame = 1

		  
Query: 9   TTVANLPIIDLSAPDRESTAATLRQACIDFGFFYIVNHGVDKDLFGRLFEESQKFFTLPL 68
           T   NLPIIDLS+PD  S+A ++RQACI+ GFFY+VNHGV+++L G +FEES K F+LPL
Sbjct: 2   TAALNLPIIDLSSPDSLSSATSIRQACIECGFFYLVNHGVEEELLGHVFEESSKLFSLPL 61

Query: 69  DDKMKIARKGIRGYTPLYHEKLDTATSTKGDSKEFLDIGPLHDEVRDSNQWPSEELLPNW 128
           +DK+K+ARK  RGYTPL+ E LD  +++KGDSKE   IGPL D   + NQWPSE++LP+W
Sbjct: 62  EDKLKLARKQHRGYTPLFSENLDPTSTSKGDSKESFYIGPL-DTRSNLNQWPSEDILPSW 121

Query: 129 KTTVEAYHDKIMKAGKRLYSLLAVALNLSENYFEDIGALDPPMPFIRLLRYPGDLSYYTN 188
           + T+E+Y+ K++ AGKRL SL+A+ALNL E +FE +GAL+ P   +RLL YPG L     
Sbjct: 122 RPTMESYYRKVLSAGKRLISLIALALNLDEEFFEKVGALNKPTAVLRLLHYPGQLGSTDE 181

Query: 189 EVLGASAHCDYGMMTLLATDGVAGLQVCREKDRQPRIWEDVLHIEGALVVNIGDLTERWT 248
           E+ GASAH DYGM+TLLA++GV GLQVCREK +QPRIWEDVLHI+GA +VNIGD+ ERWT
Sbjct: 182 EIYGASAHSDYGMVTLLASNGVPGLQVCREKSKQPRIWEDVLHIDGAFIVNIGDMLERWT 241

Query: 249 NNVFRSTLHRVVPTGQERYSVAFYLDPSHDLMVECLETCCSESNPPRFPPFRYIDYLLER 308
           N +FRSTLHRV+P GQERYSVAF+LDP+ D +VECL++CCSE++PPRFPP R  DYL ER
Sbjct: 242 NCLFRSTLHRVMPAGQERYSVAFFLDPNEDFVVECLKSCCSEASPPRFPPIRSGDYLKER 301

Query: 309 FEGTF 314
           F  T+
Sbjct: 302 FRLTY 305

[Close]

BLAST of Spo25880.1 vs. UniProtKB/TrEMBL
Match: A0A067KUL8_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_03437 PE=3 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 1.100e-115
Identity = 195/302 (64.57%), Postives = 244/302 (80.79%), Query Frame = 1

		  
Query: 12  ANLPIIDLSAPDRESTAATLRQACIDFGFFYIVNHGVDKDLFGRLFEESQKFFTLPLDDK 71
           A LP+IDLS+PDR +TA ++RQAC+++GFFY+VNHGV+++L  R+FEES KFF+LPLD+K
Sbjct: 5   AKLPVIDLSSPDRFATANSIRQACVEYGFFYLVNHGVEEELLSRVFEESSKFFSLPLDEK 64

Query: 72  MKIARKGIRGYTPLYHEKLDTATSTKGDSKEFLDIGPLHDEVRDSNQWPSEELLPNWKTT 131
           MK+ RK  RGYTPL+ E LD ++ +KGDSKE   IGPL     + NQWPSEE LP+W+ T
Sbjct: 65  MKLPRKEHRGYTPLHAENLDPSSGSKGDSKEGFYIGPLEKNA-NLNQWPSEEDLPSWRLT 124

Query: 132 VEAYHDKIMKAGKRLYSLLAVALNLSENYFEDIGALDPPMPFIRLLRYPGDLSYYTNEVL 191
           +EAY++K+M A KRL SL+A+ALNL E+YFE +GALD P  F+RLL YPGDL     E+ 
Sbjct: 125 LEAYYNKVMSAAKRLVSLIALALNLDEHYFEKVGALDNPQGFLRLLHYPGDLGNPEEEIF 184

Query: 192 GASAHCDYGMMTLLATDGVAGLQVCREKDRQPRIWEDVLHIEGALVVNIGDLTERWTNNV 251
           GASAH DYGM+TLLATDGV GLQVCREK ++PRIWE+V H+ GA +VNIGD+TERWTN +
Sbjct: 185 GASAHSDYGMITLLATDGVPGLQVCREKFKEPRIWENVSHMPGAFIVNIGDMTERWTNCL 244

Query: 252 FRSTLHRVVPTGQERYSVAFYLDPSHDLMVECLETCCSESNPPRFPPFRYIDYLLERFEG 311
           FRSTLHRV+PTGQERYS+AF+LDP+ D +V+CLE+CCSES P RFPP    DYL ER   
Sbjct: 245 FRSTLHRVMPTGQERYSLAFFLDPNPDCIVQCLESCCSESFPQRFPPICSGDYLQERLRV 304

Query: 312 TF 314
           T+
Sbjct: 305 TY 305

[Close]

BLAST of Spo25880.1 vs. ExPASy Swiss-Prot
Match: Y3291_DICDI (Probable iron/ascorbate oxidoreductase DDB_G0283291 OS=Dictyostelium discoideum GN=DDB_G0283291 PE=3 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 2.700e-33
Identity = 108/309 (34.95%), Postives = 155/309 (50.16%), Query Frame = 1

		  
Query: 10  TVANLPIIDLSA--PDREST------AATLRQACIDFGFFYIVNHGVDKDLFGRLFEESQ 69
           T+  LPIID+ +   D+E        +  +  AC +FGFFYI  HG+D++L  RL   S+
Sbjct: 9   TIEQLPIIDIESYGNDKEEELKKKLISKEIENACKNFGFFYIKGHGIDQELIDRLERLSK 68

Query: 70  KFFTLPLDDKMK----IARKGIRGYTPLYHEKLDTATSTKGDSKEFLDIG-PLHDE---- 129
           KFF+L    KMK    +A+K  RGY  +  E     TS   D KE L +G  L+D+    
Sbjct: 69  KFFSLDQSIKMKYRMELAQKAWRGYFVVGGE----LTSGLKDWKEGLYLGTELNDDHPLV 128

Query: 130 -----VRDSNQWPS--EEL---LPNWKTTVEAYHDKIMKAGKRLYSLLAVALNLSENYFE 189
                +   N +P+  EE+   +  +K T+  Y DK+ K G  L  L+A++LNLS +YF 
Sbjct: 129 IAQTPLHGLNLFPTLEEEIEYDIVGFKDTILTYIDKVTKLGHSLMELIAISLNLSADYFS 188

Query: 190 DIGALDPPMPFIRLLRYP-----GDLSYYTNE--------VLGASAHCDYGMMTLLATDG 249
                D P+   R+  YP     GD +  T E          G   H DYG++T+L  D 
Sbjct: 189 SRYTKD-PLILYRIFNYPSIISSGDDNKTTGESSDDNDKVEWGVGEHTDYGVLTILYQDD 248

Query: 250 VAGLQVCREKDRQPRIWEDVLHIEGALVVNIGDLTERWTNNVFRSTLHRVV--PTGQERY 277
           V GLQV  +       W     I+G  V NIGD+ +R T  ++RST HRV    +G++R 
Sbjct: 249 VGGLQVHSKNG-----WISAPPIKGTFVCNIGDMLDRMTGGLYRSTPHRVELNRSGRDRI 307

[Close]

BLAST of Spo25880.1 vs. ExPASy Swiss-Prot
Match: DIOX2_ARATH (Probable 2-oxoglutarate-dependent dioxygenase At3g49630 OS=Arabidopsis thaliana GN=At3g50210 PE=2 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 6.200e-30
Identity = 98/310 (31.61%), Postives = 149/310 (48.06%), Query Frame = 1

		  
Query: 9   TTVANLPIIDLSA-------PDRESTAAT------LRQACIDFGFFYIVNHGVDKDLFGR 68
           T   +LP+ID+S        PD             L +AC D GFFY++ HG+ +D+  +
Sbjct: 3   TDFKSLPVIDISRLLLKCDDPDMAEDVGVAEVVQQLDKACRDAGFFYVIGHGISEDVINK 62

Query: 69  LFEESQKFFTLPLDDKMKI---ARKGIRGY----------TPLYHEKLDTATSTKGDSKE 128
           + E +++FF LP ++K+KI      G RGY           P  HE +D     K    +
Sbjct: 63  VREITREFFKLPYEEKLKIKMTPAAGYRGYQRIGENVTKGIPDIHEAIDCYREIK--QGK 122

Query: 129 FLDIGPLHDEVRDSNQWPSEELLPNWKTTVEAYHDKIMKAGKRLYSLLAVALNLSENYFE 188
           + DIG +   +   NQWP  E    +K  +E Y        +++   +++AL  S   FE
Sbjct: 123 YGDIGKV---MEGPNQWP--ENPQEFKELMEEYIKLCTDLSRKILRGISLALAGSPYEFE 182

Query: 189 DIGALDPPMPFIRLLRYPGDLSYYTN----EVLGASAHCDYGMMTLLATDGVAGLQVCRE 248
              A D P   +RL+ YPG  + +TN      +G  AH DYG++TL+  D        R 
Sbjct: 183 GKMAGD-PFWVMRLIGYPG--AEFTNGQPENDIGCGAHTDYGLLTLVNQDDDKTALQVRN 242

Query: 249 KDRQPRIWEDVLHIEGALVVNIGDLTERWTNNVFRSTLHRVVPTG-QERYSVAFYLDPSH 288
              +   W   + I G+ V NIGD+ +  +N V+ STLHRV+    Q R  VAF+ + + 
Sbjct: 243 LGGE---WISAIPIPGSFVCNIGDMLKILSNGVYESTLHRVINNSPQYRVCVAFFYETNF 299

[Close]

BLAST of Spo25880.1 vs. ExPASy Swiss-Prot
Match: ACCO1_DICDI (1-aminocyclopropane-1-carboxylate oxidase OS=Dictyostelium discoideum GN=aco PE=2 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 8.100e-30
Identity = 88/308 (28.57%), Postives = 148/308 (48.05%), Query Frame = 1

		  
Query: 11  VANLPIIDLSA-----PDRESTAATLRQACIDFGFFYIVNHGVDKDLFGRLFEESQKFFT 70
           +  LPIID+S+      D+   A  + +AC ++GFFYI NHGVD++L   L    +KFF+
Sbjct: 5   IEKLPIIDISSFQNNENDKNQVAKEINKACKEYGFFYIKNHGVDQELIENLQNVIKKFFS 64

Query: 71  LPLDDKMK----IARKGIRGYTPLYHE----KLDTATSTKGDSKEFLDIGPLHD-EVRDS 130
           LPL+ KMK    +  +   G+  +  E    ++D        S+   DI  +H+  +  +
Sbjct: 65  LPLEIKMKWKMGLTNREWLGFFKVGQEITYGQVDWKEGCYYSSEMDGDINTIHNVPLYPT 124

Query: 131 NQWPSEELLPNWKTTVEAYHDKIMKAGKRLYSLLAVALNLSENYFEDIGALDPPMPFIRL 190
            +   +  +  +K+T+  Y +K+    +++   ++++LNL ++YF      D P   + L
Sbjct: 125 AEQEEQYEIQGFKSTIHTYIEKLTHLSQQIIEAISLSLNLPQDYFFKNYTYD-PFILMGL 184

Query: 191 LRYPG--------------------------DLSYYTNEVLGASAHCDYGMMTLLATDGV 250
           L YP                           +      E  G   H D+G++T+L  D V
Sbjct: 185 LHYPSFHHQEQEEEQEDDESNNGGKKSPNPDESKKPEVEKFGTGQHTDWGLLTVLYQDDV 244

Query: 251 AGLQVCREKDRQPRIWEDVLHIEGALVVNIGDLTERWTNNVFRSTLHRVV--PTGQERYS 277
            GLQV   K +    + D   I G  + NIGD+ ++ T   + S LHRV    +G++R+S
Sbjct: 245 GGLQV---KSKNSEEYIDAPPIPGTFICNIGDMLDKMTGGYYLSNLHRVKYNKSGRDRFS 304

[Close]

BLAST of Spo25880.1 vs. ExPASy Swiss-Prot
Match: ACCO1_DICMU (1-aminocyclopropane-1-carboxylate oxidase OS=Dictyostelium mucoroides GN=aco PE=3 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 8.100e-30
Identity = 88/308 (28.57%), Postives = 148/308 (48.05%), Query Frame = 1

		  
Query: 11  VANLPIIDLSA-----PDRESTAATLRQACIDFGFFYIVNHGVDKDLFGRLFEESQKFFT 70
           +  LPIID+S+      D+   A  + +AC ++GFFYI NHGVD++L   L    +KFF+
Sbjct: 5   IEKLPIIDISSFQNNENDKNQVAKEINKACKEYGFFYIKNHGVDQELIENLQNVIKKFFS 64

Query: 71  LPLDDKMK----IARKGIRGYTPLYHE----KLDTATSTKGDSKEFLDIGPLHD-EVRDS 130
           LPL+ KMK    +  +   G+  +  E    ++D        S+   DI  +H+  +  +
Sbjct: 65  LPLEIKMKWKMGLTNREWLGFFKVGQEITYGQVDWKEGCYYSSEMDGDINTIHNVPLYPT 124

Query: 131 NQWPSEELLPNWKTTVEAYHDKIMKAGKRLYSLLAVALNLSENYFEDIGALDPPMPFIRL 190
            +   +  +  +K+T+  Y +K+    +++   ++++LNL ++YF      D P   + L
Sbjct: 125 AEQEEQYEIQGFKSTIHTYIEKLTHLSQQIIEAISLSLNLPQDYFFKNYTYD-PFILMGL 184

Query: 191 LRYPG--------------------------DLSYYTNEVLGASAHCDYGMMTLLATDGV 250
           L YP                           +      E  G   H D+G++T+L  D V
Sbjct: 185 LHYPSFHHQEQEEEQEDDESNNGGKKSPNPDESKKPEVEKFGTGQHTDWGLLTVLYQDDV 244

Query: 251 AGLQVCREKDRQPRIWEDVLHIEGALVVNIGDLTERWTNNVFRSTLHRVV--PTGQERYS 277
            GLQV   K +    + D   I G  + NIGD+ ++ T   + S LHRV    +G++R+S
Sbjct: 245 GGLQV---KSKNSEEYIDAPPIPGTFICNIGDMLDKMTGGYYLSNLHRVKYNKSGRDRFS 304

[Close]

BLAST of Spo25880.1 vs. ExPASy Swiss-Prot
Match: FLS_MATIN (Flavonol synthase/flavanone 3-hydroxylase (Fragment) OS=Matthiola incana PE=2 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 9.000e-29
Identity = 78/293 (26.62%), Postives = 138/293 (47.10%), Query Frame = 1

		  
Query: 14  LPIIDLSAPDRESTAATLRQACIDFGFFYIVNHGVDKDLFGRLFEESQKFFTLPLDDKMK 73
           +P++DLS PD E  A T+ +A  D+G F +VNHG+  +L  RL +  ++FF LP  +K  
Sbjct: 2   VPVVDLSCPDEELVARTVVKASEDWGVFQVVNHGIPTELIQRLQKVGREFFELPEAEKRS 61

Query: 74  IARKGIRGYTPLYHEKLDTATSTKGDSKEFLDIGPLHDEVRDSNQWPSEELLPNWKTTVE 133
            AR+   G    Y  +++     +    + + +        +   WP     P+++   E
Sbjct: 62  CAREA--GSVEGYGRRIELDIKKRKGIVDQIYLSTWPPSSVNYRYWPKSP--PDYREVNE 121

Query: 134 AYHDKIMKAGKRLYSLLAVALNLSENYFEDIGALDPPMPFIRLLRYPGDLSYYTNEVLGA 193
            Y   +    +++   L+  L L     +++      M       YP     +++   G 
Sbjct: 122 EYARHVKTLSEKIMEWLSEGLGLGREAIKEVNGCWYVMNINHYPPYP-----HSDSFNGL 181

Query: 194 SAHCDYGMMTLLATDGVAGLQVCREKDRQPRIWEDVLHIEGALVVNIGDLTERWTNNVFR 253
             H D   +TL+ T+ + GLQV ++       W +V +I  A++VNIGD     +N  ++
Sbjct: 182 EPHTDINGLTLIITNEIPGLQVFKDDH-----WIEVEYIPSAIIVNIGDQIMMLSNGKYK 241

Query: 254 STLHR-VVPTGQERYSVAFYLDPSHDLMVECLETCCSESNPPRFPPFRYIDYL 306
           + LH+  V   + R S    + P++D++V  L    SE +PP+F P  Y DY+
Sbjct: 242 NVLHKTTVDKEKTRMSWPVLVSPTYDMVVGPLPELTSEDDPPKFKPIAYKDYV 280

[Close]

BLAST of Spo25880.1 vs. TAIR (Arabidopsis)
Match: AT4G16770.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 375.9 bits (964), Expect = 2.400e-104
Identity = 171/305 (56.07%), Postives = 226/305 (74.10%), Query Frame = 1

		  
Query: 10  TVANLPIIDLSAPDRESTAATLRQACIDFGFFYIVNHGVDKDLFGRLFEESQKFFTLPLD 69
           T   LPIIDLS+P++ ST+  +RQAC+D GFFY+ NHGV ++L   +  ES+K F+LPLD
Sbjct: 13  TALKLPIIDLSSPEKLSTSRLIRQACLDHGFFYLTNHGVSEELMEGVLIESKKLFSLPLD 72

Query: 70  DKMKIARKGIRGYTPLYHEKLDTATSTKGDSKEFLDIGPLHDEVRD--SNQWPSEELLPN 129
           +KM +AR G RGY+PLY EKL++++++ GDSKE    G     +     N+WP EELLP 
Sbjct: 73  EKMVMARHGFRGYSPLYDEKLESSSTSIGDSKEMFTFGSSEGVLGQLYPNKWPLEELLPL 132

Query: 130 WKTTVEAYHDKIMKAGKRLYSLLAVALNLSENYFEDIGALDPPMPFIRLLRYPGDLSYYT 189
           W+ T+E Y+  +M  GK+L+ L+A+ALNL ENYFE +GA +     +RLLRY G+ +   
Sbjct: 133 WRPTMECYYKNVMDVGKKLFGLVALALNLEENYFEQVGAFNDQAAVVRLLRYSGESNSSG 192

Query: 190 NEVLGASAHCDYGMMTLLATDGVAGLQVCREKDRQPRIWEDVLHIEGALVVNIGDLTERW 249
            E  GASAH D+GM+TLLATDGVAGLQVCR+KD++P++WEDV  I+G  VVNIGDL ERW
Sbjct: 193 EETCGASAHSDFGMITLLATDGVAGLQVCRDKDKEPKVWEDVAGIKGTFVVNIGDLMERW 252

Query: 250 TNNVFRSTLHRVVPTGQERYSVAFYLDPSHDLMVECLETCCSESNPPRFPPFRYIDYLLE 309
           TN +FRSTLHRVV  G+ER+SVA ++DP  + +VECLE+CCSE++PP+FPP R  DY  E
Sbjct: 253 TNGLFRSTLHRVVSVGKERFSVAVFVDPDPNCVVECLESCCSETSPPKFPPVRARDYFHE 312

Query: 310 RFEGT 313
           RF  T
Sbjct: 313 RFSQT 317

[Close]

BLAST of Spo25880.1 vs. TAIR (Arabidopsis)
Match: AT4G16765.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 301.2 bits (770), Expect = 7.400e-82
Identity = 138/244 (56.56%), Postives = 179/244 (73.36%), Query Frame = 1

		  
Query: 72  MKIARKGIRGYTPLYHEKLDTATSTKGDSKEFLDIGPLHDEV--RDSNQWPSEELLPNWK 131
           M + R+ + GYTPLY EKLD + S+ GDSKE    G L   +  R  NQWPSE +LP+W+
Sbjct: 1   MALLRRDLLGYTPLYAEKLDPSLSSIGDSKESFYFGSLEGVLAQRYPNQWPSEGILPSWR 60

Query: 132 TTVEAYHDKIMKAGKRLYSLLAVALNLSENYFEDIGALDPPMPFIRLLRYPGDLSYYTNE 191
            T+E Y+  ++  G++L  L+A+AL+L E++FE +GAL+ P   +RLLRYPG++     E
Sbjct: 61  QTMETYYKNVLSVGRKLLGLIALALDLDEDFFEKVGALNDPTAVVRLLRYPGEVISSDVE 120

Query: 192 VLGASAHCDYGMMTLLATDGVAGLQVCREKDRQPRIWEDVLHIEGALVVNIGDLTERWTN 251
             GASAH DYGM+TLL TDGV GLQVCR+K +QP IWEDV  I+GA +VNIGD+ ERWTN
Sbjct: 121 TYGASAHSDYGMVTLLLTDGVPGLQVCRDKSKQPHIWEDVPGIKGAFIVNIGDMMERWTN 180

Query: 252 NVFRSTLHRVVPTGQERYSVAFYLDPSHDLMVECLETCCSESNPPRFPPFRYIDYLLERF 311
            +FRSTLHRV+P G+ERYSV F+LDP+ D  V+CLE+CCSE+ PPRFPP    DY+ ERF
Sbjct: 181 GLFRSTLHRVMPVGKERYSVVFFLDPNPDCNVKCLESCCSETCPPRFPPILAGDYIKERF 240

Query: 312 EGTF 314
             T+
Sbjct: 241 RLTY 244

[Close]

BLAST of Spo25880.1 vs. TAIR (Arabidopsis)
Match: AT1G35190.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 301.2 bits (770), Expect = 7.400e-82
Identity = 144/315 (45.71%), Postives = 204/315 (64.76%), Query Frame = 1

		  
Query: 7   TTTTVANLPIIDLSAPDRESTAATLRQACIDFGFFYIVNHGVDKDLFGRLFEESQKFFTL 66
           TT  V++L  IDL+  D   +  +L+QAC+D GFFY++NHG+ ++    +FE+S+K F L
Sbjct: 5   TTMKVSSLNCIDLANDDLNHSVVSLKQACLDCGFFYVINHGISEEFMDDVFEQSKKLFAL 64

Query: 67  PLDDKMKIAR-KGIRGYTPLYHEKLDTATSTKGDSKEFLDIG---PLHDEVRDS-----N 126
           PL++KMK+ R +  RGYTP+  E LD      GD KE   IG   P  D   D      N
Sbjct: 65  PLEEKMKVLRNEKHRGYTPVLDELLDPKNQINGDHKEGYYIGIEVPKDDPHWDKPFYGPN 124

Query: 127 QWPSEELLPNWKTTVEAYHDKIMKAGKRLYSLLAVALNLSENYFEDIGALDPPMPFIRLL 186
            WP  ++LP W+ T+E YH + ++    +  LLA+AL+L   YF+    L  P+  +RLL
Sbjct: 125 PWPDADVLPGWRETMEKYHQEALRVSMAIARLLALALDLDVGYFDRTEMLGKPIATMRLL 184

Query: 187 RYPGDLSYYTNEVLGASAHCDYGMMTLLATDGVAGLQVCREKDRQPRIWEDVLHIEGALV 246
           RY G +S  +  +    AH D+GMMTLLATDGV GLQ+C++K+  P+ WE V  I+GA +
Sbjct: 185 RYQG-ISDPSKGIYACGAHSDFGMMTLLATDGVMGLQICKDKNAMPQKWEYVPPIKGAFI 244

Query: 247 VNIGDLTERWTNNVFRSTLHRVVPTGQERYSVAFYLDPSHDLMVECLETCCSESNPPRFP 306
           VN+GD+ ERW+N  F+STLHRV+  GQERYS+ F+++P+HD +VECL TC SES  P++P
Sbjct: 245 VNLGDMLERWSNGFFKSTLHRVLGNGQERYSIPFFVEPNHDCLVECLPTCKSESELPKYP 304

Query: 307 PFRYIDYLLERFEGT 313
           P +   YL +R+E T
Sbjct: 305 PIKCSTYLTQRYEET 318

[Close]

BLAST of Spo25880.1 vs. TAIR (Arabidopsis)
Match: AT3G46490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 280.8 bits (717), Expect = 1.000e-75
Identity = 135/315 (42.86%), Postives = 199/315 (63.17%), Query Frame = 1

		  
Query: 5   EETTTTVANLPIIDLSAPDRESTAATLRQACIDFGFFYIVNHGVDKDLFGRLFEESQKFF 64
           E +   V++L  IDL   D   +A  L+QAC+D GFFY++NHG+ ++L    FE S+KFF
Sbjct: 8   EVSIIKVSSLTCIDLDNSDLHQSAVLLKQACLDSGFFYVINHGISEELKDEAFEHSKKFF 67

Query: 65  TLPLDDKMKIAR-KGIRGYTPLYHEKLDTATSTKGDSKEFLDIG-------PLHDEVRDS 124
            LPL++KMK+ R +  RGY P +   LD     +GD KE   IG       P  D+   S
Sbjct: 68  ALPLEEKMKVLRNEKYRGYAPFHDSLLDPENQVRGDYKEGFTIGFEGSKDGPHWDKPFHS 127

Query: 125 -NQWPSEELLPNWKTTVEAYHDKIMKAGKRLYSLLAVALNLSENYFEDIGALDPPMPFIR 184
            N WP+ ++LP W+ T+E Y+ + ++  K +  ++A+AL+L  +YF     L  P+  + 
Sbjct: 128 PNIWPNPDVLPGWRETMEKYYQEALRVCKSIAKIMALALDLDVDYFNTPEMLGNPIADMV 187

Query: 185 LLRYPGDLSYYTNEVLGASAHCDYGMMTLLATDGVAGLQVCREKDRQPRIWEDVLHIEGA 244
           L  Y G  S  +  +    AH D+GMM+LLATDGV GLQ+C++KD +P+ WE    I+GA
Sbjct: 188 LFHYEGK-SDPSKGIYACGAHSDFGMMSLLATDGVMGLQICKDKDVKPQKWEYTPSIKGA 247

Query: 245 LVVNIGDLTERWTNNVFRSTLHRVVPTGQERYSVAFYLDPSHDLMVECLETCCSESNPPR 304
            +VN+GDL ERW+N  F+STLHRV+  GQ+RYS+ F+L PSHD ++ECL TC SE+N P+
Sbjct: 248 YIVNLGDLLERWSNGYFKSTLHRVLGNGQDRYSIPFFLKPSHDCIIECLPTCQSENNLPK 307

Query: 305 FPPFRYIDYLLERFE 311
           +P  +   Y+ +R++
Sbjct: 308 YPAIKCSTYISQRYK 321

[Close]

BLAST of Spo25880.1 vs. TAIR (Arabidopsis)
Match: AT3G46480.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 250.4 bits (638), Expect = 1.500e-66
Identity = 128/307 (41.69%), Postives = 185/307 (60.26%), Query Frame = 1

		  
Query: 5   EETTTTVANLPIIDLSAPDRESTAATLRQACIDFGFFYIVNHGVDKDLFGRLFEESQKFF 64
           E +T  V++L  IDL+ P+ + +A +L+Q                          S+KFF
Sbjct: 8   EASTIKVSSLTCIDLANPNFQQSAVSLKQ--------------------------SKKFF 67

Query: 65  TLPLDDKMKIAR-KGIRGYTPLYHEKLDTATSTKGDSKEFLDIGPLHDEVRDSNQWPSEE 124
            LPL++KMK+ R +  RGY+P+  + LD      GD KE   IG              E 
Sbjct: 68  ALPLEEKMKVLRNEKHRGYSPVLDQILDPENQVDGDYKESFFIG-------------IEV 127

Query: 125 LLPNWKTTVEAYHDKIMKAGKRLYSLLAVALNLSENYFEDIGALDPPMPFIRLLRYPGDL 184
           +LP W+ T+E YH + ++  K +  LLA+AL+L  NYF+    L  P+  +RLLRY G +
Sbjct: 128 VLPGWRATMEKYHQEALRVCKAIARLLALALDLDTNYFDKPEMLGNPIAVMRLLRYEG-M 187

Query: 185 SYYTNEVLGASAHCDYGMMTLLATDGVAGLQVCREKDRQPRIWEDVLHIEGALVVNIGDL 244
           S     + G  AH DYGM+TLLATD V GLQ+C++KD +PR WE V  I+GA +VN+GDL
Sbjct: 188 SDPLKGIFGCGAHSDYGMLTLLATDSVTGLQICKDKDVKPRKWEYVPSIKGAYIVNLGDL 247

Query: 245 TERWTNNVFRSTLHRVVPTGQERYSVAFYLDPSHDLMVECLETCCSESNPPRFPPFRYID 304
            ERW+N +F+STLHRV+  GQ+RYS+ F+++PSHD +VECL TC SE+N P++P  +   
Sbjct: 248 LERWSNGIFKSTLHRVLGNGQDRYSIPFFIEPSHDCLVECLPTCQSENNLPKYPAIKCST 274

Query: 305 YLLERFE 311
           +L +R++
Sbjct: 308 FLTQRYQ 274

The following BLAST results are available for this feature:

BLAST of Spo25880.1 vs. NCBI nr
BLAST of Spo25880.1 vs. UniProtKB/TrEMBL
BLAST of Spo25880.1 vs. ExPASy Swiss-Prot
BLAST of Spo25880.1 vs. TAIR (Arabidopsis)

BLAST of Spo25880.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
gi\|902237595\|gb\|KNA24851.1\|	4.6e-134	72.7	hypothetical protein SOVF_0118...	[more]
gi\|731332211\|ref\|XP_010677081.1\|	7.9e-134	72.2	PREDICTED: flavonol synthase/f...	[more]
gi\|731332215\|ref\|XP_010677083.1\|	6.4e-128	69.0	PREDICTED: probable iron/ascor...	[more]
gi\|694433837\|ref\|XP_009344185.1\|	8.7e-117	62.5	PREDICTED: 1-aminocyclopropane...	[more]
gi\|595847723\|ref\|XP_007209384.1\|	3.3e-116	63.6	hypothetical protein PRUPE_ppa...	[more]

BLAST of Spo25880.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
A0A0K9RZB1_SPIOL	3.2e-134	72.7	Uncharacterized protein OS=Spi...	[more]
A0A0J8CD92_BETVU	5.5e-134	72.2	Uncharacterized protein OS=Bet...	[more]
A0A0J8F728_BETVU	4.5e-128	69.0	Uncharacterized protein OS=Bet...	[more]
M5WMS3_PRUPE	2.3e-116	63.6	Uncharacterized protein OS=Pru...	[more]
A0A067KUL8_JATCU	1.1e-115	64.5	Uncharacterized protein OS=Jat...	[more]

BLAST of Spo25880.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
Y3291_DICDI	2.7e-33	34.9	Probable iron/ascorbate oxidor...	[more]
DIOX2_ARATH	6.2e-30	31.6	Probable 2-oxoglutarate-depend...	[more]
ACCO1_DICDI	8.1e-30	28.5	1-aminocyclopropane-1-carboxyl...	[more]
ACCO1_DICMU	8.1e-30	28.5	1-aminocyclopropane-1-carboxyl...	[more]
FLS_MATIN	9.0e-29	26.6	Flavonol synthase/flavanone 3-...	[more]

BLAST of Spo25880.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
AT4G16770.1	2.4e-104	56.0	2-oxoglutarate (2OG) and Fe(II...	[more]
AT4G16765.1	7.4e-82	56.5	2-oxoglutarate (2OG) and Fe(II...	[more]
AT1G35190.1	7.4e-82	45.7	2-oxoglutarate (2OG) and Fe(II...	[more]
AT3G46490.1	1.0e-75	42.8	2-oxoglutarate (2OG) and Fe(II...	[more]
AT3G46480.1	1.5e-66	41.6	2-oxoglutarate (2OG) and Fe(II...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002283	Isopenicillin N synthase	PRINTS	PR00682	IPNSYNTHASE	coord: 263..281 score: 1.6E-11coord: 31..48 score: 1.6E-11coord: 236..262 score: 1.6E-11coord: 145..161 score: 1.6
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PFAM	PF03171	2OG-FeII_Oxy	coord: 177..273 score: 3.6
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROFILE	PS51471	FE2OG_OXY	coord: 168..275 score: 11
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 14..122 score: 1.5
IPR027443	Isopenicillin N synthase-like	GENE3D	2.60.120.330		coord: 10..314 score: 1.2
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 13..314 score: 2.5E
None	No IPR available	PANTHER	PTHR10209:SF214	SUBFAMILY NOT NAMED	coord: 13..314 score: 2.5E
None	No IPR available	unknown	SSF51197	Clavaminate synthase-like	coord: 9..313 score: 9.61

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0009607	response to biotic stimulus
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016706	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0005515	protein binding
molecular_function	GO:0016491	oxidoreductase activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo08095	0.69	Barchart \| Table
Spo13051	0.68	Barchart \| Table