Spo26569 (gene)

Overview
NameSpo26569
Typegene
OrganismSpinacia oleracea (Spinach)
DescriptionChalcone-flavonone isomerase family protein
LocationSpoScf_01513 : 61945 .. 64797 (+)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAAAGTAGTAAGTATAAGTTGTATATTCATATCTTTTGTTAAATATGGTTCAAATATTTAAAGGAGAAATAAATATACCCGAAGTTCCATATATAATATGGACATACGGTGTATATTTAAGAGCTCGTACCATACATAAGGGCCCCTTTGGAGCACTTTTATCGCGATTCGCCCTGAGCTCCTAAAATATCAGGATCGACCCTGTCCATTTCCACCATTTATTACGTGCTACCATACAGACCGTTAAGGTACAAAGTAATAATGAAGTCGCTCTATTTTTATTTCATCAAAAGTTCAAAACTAAAATCTAGGTAAAATAATCTTTTTATGTTTCTCCTTCAACATATGTGGGCCCGTATAATCGTAGTCCACATAAAGGAAAATGTCATTGAAGGAGGGCGAAAACAGAATTAAAGAAAAGCAACGAAAAATTGACTTGAAAACTCAGAACGTAGTGAAGTAAGCAACCTCTCATAAGCTTACCTACCTCCTTTGCCTTTGAATGTTAGGTACCACTCTATTTATATTTCACTCATTTTGTAACATTCAACTTCTTTAATACAAGCATCATTTACTTTACATACTCCTTCATCAATCGTTAATCTCTTCTCAATTCTACGATAATCAGTAAGTACTTCTGTTTAATCTCCTGATTTACTAGATTTTCGTTTTATTTATTTGTTTTTATTTATGTTTTCAATGTGATAAAGACTGTCCAAAGTTCAAGTTGTTCATCATTAATGATATTTTATTATTAATCATACTTACACACACCATTTATGTGTCTATGTTAATACTCTTGGTTCATTTGTATGAATGACATGGCATCTTGTAATCTTGTTAAGTGTATCAATATAGTGGAGATGTCGTATGACCATTATTTAAGGGAAGTTTTTTACAAATCCGATCCTCTGATCCGTGGATCATTACTAGATTGTTTGATTGAAGGTTTGCTTAAAACCCCAAGTTGGATCTTTTCAAGTTATTTATAAACAACCTTAACTAAATAAATTTGTTAAAACGGTCCTGAATTTTACTTATGTTCAATCAACCCCCATATATATAAAAGGAATATTTAAATGAACAAATAAATTTAAAAAATATTGAGTGGTTGTTTACATAACTACACTACACTATAGGTCGAGTTTGTGTTGAGATAAGTGCGCGTGTGCCAATGTCAACTAGTCTAACTGAGCTGATAAAATATCTGAATGGGTATCCAAAAACTTTGGATCTGAGTCTTTTATATACGGATCCAGTGTCCCTTACGACTTTCTATGCATGTAAATAGTAGGTACCTTAAGTCCTAAATTCCAATTGTATCCATAAATTGTAGTACGTGACTAGGTGTTGGTGCATTATGTGGATGAAAAGAGTAACAAAAACTTGATACCCTTTGTTTTTGAATGGTATGACTAATTAGATTATTTACTGATGATGCAGTGGGAAACGATGTAATAGTGGATGATATTGCTTTTGCTCCTCAAGTTACATTAACCAAATCGCTACCCCTCTTGGGTTATGGTAAGAGATTCAACTTAATCAAGCTTGATTGTATTATTACTGATCAAGAATTTATTTGAACAAAACTAACTTGTTTCCTGAATTATTTTGACTTAATCGCGTAAACCAATTTACATACATAGGATGATCCTGTCCAAAGTTTTCATGAGATTCTTATCAATCCATATTAGAAATCAACTGAAAGCATTCATAAGAATATCAATCCGTTTAGATGGTGAAATTGCTTTAGTAGTTGTTAAGTGTGGTACGAAAAGTGAGCGTTCTGTTATGGTTATGGACTGAACAGGGTGCACAGAACTTGAAATCCACTTCCTGCAAATAAAGTTCACAGCAATTGGAGTGTACATGGAGAGGGAAGTGGTACAACACTTGCAGAAATGGAAGGGTAAATCAGGAAAGGAGCTCGCCGATGATGATGATTTCTTTGATGCTCTTACTGCTGGTAAAACCAGTAGCATTTTTATTTTATATTTACTCCTACATTTATCAAGTTCAGAACAATGCTGATGTAGGTTGTTGTGAATATTTTGTGCAGCCCCTGTGGAAACAGTAATTAAAGCTGTGGTGATCAAAGAAATTAAGGCTTCACAATTAGGCACACAGCTGGAGGGTTCAGTAAGGGACCGATTGGCAGCTGCAGACAAGTATGAAGAAGAGGAAGAAGCTGCACTTGAGAAGGTTGCCGAGTTCTTCCCCACCAAATACCTTAAGAAAGACTCTTTCTTCACTTTCAGCTTCTTTGCTTCAGGCACAGCTCAGGTTAACTTGTTATTCATTTATCCTTGAAGAATTCAACTCCATATAAGTAAGGTCAGTCAGCTGTTAACAGATATTCGAATTTTATTGACAGATAACTATCACCACGGATGAGAAAGAAGAAACAACGACAGCCGTGGAGAACAAAAATGTGGTGGAGATGATAAAGAAATGGTATTTAGGTGGAAGCAGGTCAGTGACTCCTTCGACTATAGAGTGCTTGTGTAACAACCTCTCAGCCGAGTTATCCAACTGAATGTAATGTAATGCAAATGAGTGTAAAAGGATCCTATTCAGATTGTGCCCTGTTTTTAATCTCTGCTAATAATACATGTCCATGTCCTCTTTGGTAATAATAGAGTGATCTTTCGTTTTTATCATTGCTTTCTGACATACCATTTGAGGCGATATCGGACAGGAATGGTAGTGTTTTCTGAGTTAGTACTAATACTAGTATATAGAAAATCAAGTATCACTTGATCCATATAGAATCCAAAAACATAACACCAGAGTGTGACCATAGCCGATTCAACCCAAACAAACCCAACCCGGACAATGAATTTTCCAC

mRNA sequence

ATGGAGAAAGTACATCATTTACTTTACATACTCCTTCATCAATCGTTAATCTCTTCTCAATTCTACGATAATCAATTATTTACTGATGATGCAGTGGGAAACGATGTAATAGTGGATGATATTGCTTTTGCTCCTCAAGTTACATTAACCAAATCGCTACCCCTCTTGGGTTATGGGTGCACAGAACTTGAAATCCACTTCCTGCAAATAAAGTTCACAGCAATTGGAGTGTACATGGAGAGGGAAGTGGTACAACACTTGCAGAAATGGAAGGGTAAATCAGGAAAGGAGCTCGCCGATGATGATGATTTCTTTGATGCTCTTACTGCTGCCCCTGTGGAAACAGTAATTAAAGCTGTGGTGATCAAAGAAATTAAGGCTTCACAATTAGGCACACAGCTGGAGGGTTCAGTAAGGGACCGATTGGCAGCTGCAGACAAGTATGAAGAAGAGGAAGAAGCTGCACTTGAGAAGGTTGCCGAGTTCTTCCCCACCAAATACCTTAAGAAAGACTCTTTCTTCACTTTCAGCTTCTTTGCTTCAGGCACAGCTCAGATAACTATCACCACGGATGAGAAAGAAGAAACAACGACAGCCGTGGAGAACAAAAATGTGGTGGAGATGATAAAGAAATGGTATTTAGGTGGAAGCAGGTCAGTGACTCCTTCGACTATAGAGTGCTTGTGTAACAACCTCTCAGCCGAGTTATCCAACTGAATGTAATGTAATGCAAATGAGTGTAAAAGGATCCTATTCAGATTGTGCCCTGTTTTTAATCTCTGCTAATAATACATGTCCATGTCCTCTTTGGTAATAATAGAGTGATCTTTCGTTTTTATCATTGCTTTCTGACATACCATTTGAGGCGATATCGGACAGGAATGGTAGTGTTTTCTGAGTTAGTACTAATACTAGTATATAGAAAATCAAGTATCACTTGATCCATATAGAATCCAAAAACATAACACCAGAGTGTGACCATAGCCGATTCAACCCAAACAAACCCAACCCGGACAATGAATTTTCCAC

Coding sequence (CDS)

ATGGAGAAAGTACATCATTTACTTTACATACTCCTTCATCAATCGTTAATCTCTTCTCAATTCTACGATAATCAATTATTTACTGATGATGCAGTGGGAAACGATGTAATAGTGGATGATATTGCTTTTGCTCCTCAAGTTACATTAACCAAATCGCTACCCCTCTTGGGTTATGGGTGCACAGAACTTGAAATCCACTTCCTGCAAATAAAGTTCACAGCAATTGGAGTGTACATGGAGAGGGAAGTGGTACAACACTTGCAGAAATGGAAGGGTAAATCAGGAAAGGAGCTCGCCGATGATGATGATTTCTTTGATGCTCTTACTGCTGCCCCTGTGGAAACAGTAATTAAAGCTGTGGTGATCAAAGAAATTAAGGCTTCACAATTAGGCACACAGCTGGAGGGTTCAGTAAGGGACCGATTGGCAGCTGCAGACAAGTATGAAGAAGAGGAAGAAGCTGCACTTGAGAAGGTTGCCGAGTTCTTCCCCACCAAATACCTTAAGAAAGACTCTTTCTTCACTTTCAGCTTCTTTGCTTCAGGCACAGCTCAGATAACTATCACCACGGATGAGAAAGAAGAAACAACGACAGCCGTGGAGAACAAAAATGTGGTGGAGATGATAAAGAAATGGTATTTAGGTGGAAGCAGGTCAGTGACTCCTTCGACTATAGAGTGCTTGTGTAACAACCTCTCAGCCGAGTTATCCAACTGA

Protein sequence

MEKVHHLLYILLHQSLISSQFYDNQLFTDDAVGNDVIVDDIAFAPQVTLTKSLPLLGYGCTELEIHFLQIKFTAIGVYMEREVVQHLQKWKGKSGKELADDDDFFDALTAAPVETVIKAVVIKEIKASQLGTQLEGSVRDRLAAADKYEEEEEAALEKVAEFFPTKYLKKDSFFTFSFFASGTAQITITTDEKEETTTAVENKNVVEMIKKWYLGGSRSVTPSTIECLCNNLSAELSN
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo26569.1Spo26569.1mRNA


Homology
[Close]
BLAST of Spo26569.1 vs. NCBI nr
Match: gi|731320056|ref|XP_010670602.1| (PREDICTED: probable chalcone--flavonone isomerase 3 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 354.4 bits (908), Expect = 1.600e-94
Identity = 179/206 (86.89%), Postives = 194/206 (94.17%), Query Frame = 1 

		  

Query: 32  VGNDVIVDDIAFAPQVTLTKSLPLLGYGCTELEIHFLQIKFTAIGVYMEREVVQHLQKWK 91
           +GNDV+VDD+AFAP+ TLTK L L GYGCTELEIH+LQIKFTAIGVY+E+EVVQHLQKWK
Sbjct: 1   MGNDVMVDDVAFAPEFTLTKPLSLFGYGCTELEIHYLQIKFTAIGVYLEKEVVQHLQKWK 60

Query: 92  GKSGKELADDDDFFDALTAAPVETVIKAVVIKEIKASQLGTQLEGSVRDRLAAADKYEEE 151
           GKSGKELADDD+FFDAL AAPVE+VIKAVVIKEIKASQLGTQLEGSVRDRLAA DKYE+E
Sbjct: 61  GKSGKELADDDEFFDALAAAPVESVIKAVVIKEIKASQLGTQLEGSVRDRLAAIDKYEDE 120

Query: 152 EEAALEKVAEFFPTKYLKKDSFFTFSFFASGTAQITITTDEKEETTTAVENKNVVEMIKK 211
           EE ALEKVAEFFPTKYLKK+S+FTFSFFASGTA+I ITTDEKEE+ T VENKNVVEMIKK
Sbjct: 121 EEVALEKVAEFFPTKYLKKNSYFTFSFFASGTAEIAITTDEKEESKTTVENKNVVEMIKK 180

Query: 212 WYLGGSRSVTPSTIECLCNNLSAELS 238
           WYLGGSRSVTPSTIECL N L+AELS
Sbjct: 181 WYLGGSRSVTPSTIECLANTLAAELS 206
[Close]
BLAST of Spo26569.1 vs. NCBI nr
Match: gi|870865722|gb|KMT16760.1| (hypothetical protein BVRB_2g042450 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 345.5 bits (885), Expect = 7.300e-92
Identity = 175/201 (87.06%), Postives = 189/201 (94.03%), Query Frame = 1 

		  

Query: 37  IVDDIAFAPQVTLTKSLPLLGYGCTELEIHFLQIKFTAIGVYMEREVVQHLQKWKGKSGK 96
           +VDD+AFAP+ TLTK L L GYGCTELEIH+LQIKFTAIGVY+E+EVVQHLQKWKGKSGK
Sbjct: 1   MVDDVAFAPEFTLTKPLSLFGYGCTELEIHYLQIKFTAIGVYLEKEVVQHLQKWKGKSGK 60

Query: 97  ELADDDDFFDALTAAPVETVIKAVVIKEIKASQLGTQLEGSVRDRLAAADKYEEEEEAAL 156
           ELADDD+FFDAL AAPVE+VIKAVVIKEIKASQLGTQLEGSVRDRLAA DKYE+EEE AL
Sbjct: 61  ELADDDEFFDALAAAPVESVIKAVVIKEIKASQLGTQLEGSVRDRLAAIDKYEDEEEVAL 120

Query: 157 EKVAEFFPTKYLKKDSFFTFSFFASGTAQITITTDEKEETTTAVENKNVVEMIKKWYLGG 216
           EKVAEFFPTKYLKK+S+FTFSFFASGTA+I ITTDEKEE+ T VENKNVVEMIKKWYLGG
Sbjct: 121 EKVAEFFPTKYLKKNSYFTFSFFASGTAEIAITTDEKEESKTTVENKNVVEMIKKWYLGG 180

Query: 217 SRSVTPSTIECLCNNLSAELS 238
           SRSVTPSTIECL N L+AELS
Sbjct: 181 SRSVTPSTIECLANTLAAELS 201
[Close]
BLAST of Spo26569.1 vs. NCBI nr
Match: gi|816196829|dbj|BAR72208.1| (enhancer of flavonoid production [Dianthus caryophyllus])

HSP 1 Score: 319.3 bits (817), Expect = 5.600e-84
Identity = 163/204 (79.90%), Postives = 181/204 (88.73%), Query Frame = 1 

		  

Query: 34  NDVIVDDIAFAPQVTLTKSLPLLGYGCTELEIHFLQIKFTAIGVYMEREVVQHLQKWKGK 93
           NDV+VDDIAFA + TLTK L LLGYGCT+LEIHFLQIK TAIGVY E  VV HLQKWKGK
Sbjct: 3   NDVLVDDIAFAKETTLTKPLSLLGYGCTDLEIHFLQIKHTAIGVYFEPTVVDHLQKWKGK 62

Query: 94  SGKELADDDDFFDALTAAPVETVIKAVVIKEIKASQLGTQLEGSVRDRLAAADKYEEEEE 153
           SGKELA+DD+FF AL +APVE+V+K VVIKEIKASQ GTQLEG+VRDRLAA DKYE+EEE
Sbjct: 63  SGKELAEDDEFFAALASAPVESVVKVVVIKEIKASQYGTQLEGAVRDRLAAVDKYEDEEE 122

Query: 154 AALEKVAEFFPTKYLKKDSFFTFSFFASGTAQITITTDEKEETTTAVENKNVVEMIKKWY 213
           AALEKVAEFFP KYLKK+SFFTF FFASGTA+IT+TT+ KE++   V N NVVEMIKKWY
Sbjct: 123 AALEKVAEFFPIKYLKKNSFFTFKFFASGTAEITMTTEAKEDSKIEVGNANVVEMIKKWY 182

Query: 214 LGGSRSVTPSTIECLCNNLSAELS 238
           LGGSR+V+PSTIECL NNLSAELS
Sbjct: 183 LGGSRAVSPSTIECLANNLSAELS 206
[Close]
BLAST of Spo26569.1 vs. NCBI nr
Match: gi|902226877|gb|KNA20513.1| (hypothetical protein SOVF_051680 [Spinacia oleracea])

HSP 1 Score: 307.4 bits (786), Expect = 2.200e-80
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 1 

		  

Query: 79  MEREVVQHLQKWKGKSGKELADDDDFFDALTAAPVETVIKAVVIKEIKASQLGTQLEGSV 138
           MEREVVQHLQKWKGKSGKELADDDDFFDALTAAPVETVIKAVVIKEIKASQLGTQLEGSV
Sbjct: 1   MEREVVQHLQKWKGKSGKELADDDDFFDALTAAPVETVIKAVVIKEIKASQLGTQLEGSV 60

Query: 139 RDRLAAADKYEEEEEAALEKVAEFFPTKYLKKDSFFTFSFFASGTAQITITTDEKEETTT 198
           RDRLAAADKYEEEEEAALEKVAEFFPTKYLKKDSFFTFSFFASGTAQITITTDEKEETTT
Sbjct: 61  RDRLAAADKYEEEEEAALEKVAEFFPTKYLKKDSFFTFSFFASGTAQITITTDEKEETTT 120

Query: 199 AVENKNVVEMIKKWYLGGSRSVTPSTIECLCNNLSAELSN 239
           AVENKNVVEMIKKWYLGGSRSVTPSTIECLCNNLSAELSN
Sbjct: 121 AVENKNVVEMIKKWYLGGSRSVTPSTIECLCNNLSAELSN 160
[Close]
BLAST of Spo26569.1 vs. NCBI nr
Match: gi|444475579|gb|AGE10598.1| (chalcone isomerase [Lonicera japonica])

HSP 1 Score: 268.5 bits (685), Expect = 1.100e-68
Identity = 137/207 (66.18%), Postives = 169/207 (81.64%), Query Frame = 1 

		  

Query: 32  VGNDVIVDDIAFAPQVTLTKSLPLLGYGCTELEIHFLQIKFTAIGVYMEREVVQHLQKWK 91
           +G++V+VD++ F PQVT+TK L LLG+G T++EIHFLQIKFTAIGVY++ E+V HLQKWK
Sbjct: 1   MGSEVMVDEVPFPPQVTVTKPLSLLGHGITDIEIHFLQIKFTAIGVYLDSEIVSHLQKWK 60

Query: 92  GKSGKELADDDDFFDALTAAPVETVIKAVVIKEIKASQLGTQLEGSVRDRLAAADKYEEE 151
           GK+G ELA+DDDFFD+L +APV+  ++ VVIKEIK SQ G QLE +VRDRLAAADKYEEE
Sbjct: 61  GKTGTELAEDDDFFDSLISAPVDKCLRVVVIKEIKGSQYGVQLESAVRDRLAAADKYEEE 120

Query: 152 EEAALEKVAEFFPTKYLKKDSFFTFSFFA-SGTAQITITTDEKEETTTAVENKNVVEMIK 211
           EE ALE++ EFF +KY KKDS  T++F A S TA+I  TT  KE++   VEN+NVVEMIK
Sbjct: 121 EEEALEQLVEFFQSKYFKKDSVITYTFPATSSTAEILFTTGGKEDSKIKVENENVVEMIK 180

Query: 212 KWYLGGSRSVTPSTIECLCNNLSAELS 238
           KWYLGG+R V+PSTI  L N LS ELS
Sbjct: 181 KWYLGGTRGVSPSTITNLANTLSVELS 207
[Close]
BLAST of Spo26569.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CSJ0_BETVU (Chalcone-flavonone isomerase family protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g042450 PE=3 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 5.100e-92
Identity = 175/201 (87.06%), Postives = 189/201 (94.03%), Query Frame = 1 

		  

Query: 37  IVDDIAFAPQVTLTKSLPLLGYGCTELEIHFLQIKFTAIGVYMEREVVQHLQKWKGKSGK 96
           +VDD+AFAP+ TLTK L L GYGCTELEIH+LQIKFTAIGVY+E+EVVQHLQKWKGKSGK
Sbjct: 1   MVDDVAFAPEFTLTKPLSLFGYGCTELEIHYLQIKFTAIGVYLEKEVVQHLQKWKGKSGK 60

Query: 97  ELADDDDFFDALTAAPVETVIKAVVIKEIKASQLGTQLEGSVRDRLAAADKYEEEEEAAL 156
           ELADDD+FFDAL AAPVE+VIKAVVIKEIKASQLGTQLEGSVRDRLAA DKYE+EEE AL
Sbjct: 61  ELADDDEFFDALAAAPVESVIKAVVIKEIKASQLGTQLEGSVRDRLAAIDKYEDEEEVAL 120

Query: 157 EKVAEFFPTKYLKKDSFFTFSFFASGTAQITITTDEKEETTTAVENKNVVEMIKKWYLGG 216
           EKVAEFFPTKYLKK+S+FTFSFFASGTA+I ITTDEKEE+ T VENKNVVEMIKKWYLGG
Sbjct: 121 EKVAEFFPTKYLKKNSYFTFSFFASGTAEIAITTDEKEESKTTVENKNVVEMIKKWYLGG 180

Query: 217 SRSVTPSTIECLCNNLSAELS 238
           SRSVTPSTIECL N L+AELS
Sbjct: 181 SRSVTPSTIECLANTLAAELS 201
[Close]
BLAST of Spo26569.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RLZ4_SPIOL (Chalcone-flavonone isomerase family protein OS=Spinacia oleracea GN=SOVF_051680 PE=3 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 1.500e-80
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 1 

		  

Query: 79  MEREVVQHLQKWKGKSGKELADDDDFFDALTAAPVETVIKAVVIKEIKASQLGTQLEGSV 138
           MEREVVQHLQKWKGKSGKELADDDDFFDALTAAPVETVIKAVVIKEIKASQLGTQLEGSV
Sbjct: 1   MEREVVQHLQKWKGKSGKELADDDDFFDALTAAPVETVIKAVVIKEIKASQLGTQLEGSV 60

Query: 139 RDRLAAADKYEEEEEAALEKVAEFFPTKYLKKDSFFTFSFFASGTAQITITTDEKEETTT 198
           RDRLAAADKYEEEEEAALEKVAEFFPTKYLKKDSFFTFSFFASGTAQITITTDEKEETTT
Sbjct: 61  RDRLAAADKYEEEEEAALEKVAEFFPTKYLKKDSFFTFSFFASGTAQITITTDEKEETTT 120

Query: 199 AVENKNVVEMIKKWYLGGSRSVTPSTIECLCNNLSAELSN 239
           AVENKNVVEMIKKWYLGGSRSVTPSTIECLCNNLSAELSN
Sbjct: 121 AVENKNVVEMIKKWYLGGSRSVTPSTIECLCNNLSAELSN 160
[Close]
BLAST of Spo26569.1 vs. UniProtKB/TrEMBL
Match: L7Z972_LONJA (Chalcone-flavonone isomerase family protein OS=Lonicera japonica GN=CHI1 PE=2 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 8.000e-69
Identity = 137/207 (66.18%), Postives = 169/207 (81.64%), Query Frame = 1 

		  

Query: 32  VGNDVIVDDIAFAPQVTLTKSLPLLGYGCTELEIHFLQIKFTAIGVYMEREVVQHLQKWK 91
           +G++V+VD++ F PQVT+TK L LLG+G T++EIHFLQIKFTAIGVY++ E+V HLQKWK
Sbjct: 1   MGSEVMVDEVPFPPQVTVTKPLSLLGHGITDIEIHFLQIKFTAIGVYLDSEIVSHLQKWK 60

Query: 92  GKSGKELADDDDFFDALTAAPVETVIKAVVIKEIKASQLGTQLEGSVRDRLAAADKYEEE 151
           GK+G ELA+DDDFFD+L +APV+  ++ VVIKEIK SQ G QLE +VRDRLAAADKYEEE
Sbjct: 61  GKTGTELAEDDDFFDSLISAPVDKCLRVVVIKEIKGSQYGVQLESAVRDRLAAADKYEEE 120

Query: 152 EEAALEKVAEFFPTKYLKKDSFFTFSFFA-SGTAQITITTDEKEETTTAVENKNVVEMIK 211
           EE ALE++ EFF +KY KKDS  T++F A S TA+I  TT  KE++   VEN+NVVEMIK
Sbjct: 121 EEEALEQLVEFFQSKYFKKDSVITYTFPATSSTAEILFTTGGKEDSKIKVENENVVEMIK 180

Query: 212 KWYLGGSRSVTPSTIECLCNNLSAELS 238
           KWYLGG+R V+PSTI  L N LS ELS
Sbjct: 181 KWYLGGTRGVSPSTITNLANTLSVELS 207
[Close]
BLAST of Spo26569.1 vs. UniProtKB/TrEMBL
Match: A0A0A1G7T9_9ERIC (Chalcone-flavonone isomerase family protein OS=Actinidia chrysantha GN=CHI PE=3 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 3.900e-68
Identity = 138/203 (67.98%), Postives = 163/203 (80.30%), Query Frame = 1 

		  

Query: 36  VIVDDIAFAPQVTLTKSLPLLGYGCTELEIHFLQIKFTAIGVYMEREVVQHLQKWKGKSG 95
           ++ D+I F PQVT TK L LLG+G T++EIHFLQIKFTAIGVY++ E+V HLQ+WKGK+G
Sbjct: 7   MVADEIPFPPQVTTTKPLSLLGHGITDIEIHFLQIKFTAIGVYLDCEIVGHLQQWKGKTG 66

Query: 96  KELADDDDFFDALTAAPVETVIKAVVIKEIKASQLGTQLEGSVRDRLAAADKYEEEEEAA 155
           K+LA+DDDFF+AL +APVE  ++ VVIKEIK SQ G QLE +VRDRLAA DKYEEEEEAA
Sbjct: 67  KDLAEDDDFFEALVSAPVEKFLRVVVIKEIKGSQYGVQLESAVRDRLAADDKYEEEEEAA 126

Query: 156 LEKVAEFFPTKYLKKDSFFTFSFFA-SGTAQITITTDEKEETTTAVENKNVVEMIKKWYL 215
           LE V EFF +KY KKDS  TF F A SGTA+I  +T+ K E+   VEN NVVEMIKKWYL
Sbjct: 127 LELVVEFFQSKYFKKDSVITFHFPASSGTAEIAFSTEGKGESKICVENANVVEMIKKWYL 186

Query: 216 GGSRSVTPSTIECLCNNLSAELS 238
           GG+R V+PSTI CL   LSAELS
Sbjct: 187 GGTRGVSPSTISCLATTLSAELS 209
[Close]
BLAST of Spo26569.1 vs. UniProtKB/TrEMBL
Match: T2DL26_ACTCH (Chalcone-flavonone isomerase family protein OS=Actinidia chinensis PE=2 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 2.000e-67
Identity = 136/203 (67.00%), Postives = 162/203 (79.80%), Query Frame = 1 

		  

Query: 36  VIVDDIAFAPQVTLTKSLPLLGYGCTELEIHFLQIKFTAIGVYMEREVVQHLQKWKGKSG 95
           ++ D+I F PQVT TK L LLG+G  ++EIHFLQIKFTAIGVY++ E+V HLQ+WKGK+G
Sbjct: 7   MVADEIPFPPQVTTTKPLSLLGHGIADIEIHFLQIKFTAIGVYLDCEIVGHLQQWKGKTG 66

Query: 96  KELADDDDFFDALTAAPVETVIKAVVIKEIKASQLGTQLEGSVRDRLAAADKYEEEEEAA 155
           K+LA+DDDFF+AL +APVE  ++ VVIKE+K SQ G QLE +VRDRLAA DKYEEEEEAA
Sbjct: 67  KDLAEDDDFFEALVSAPVEKFLRVVVIKEVKGSQYGVQLESAVRDRLAADDKYEEEEEAA 126

Query: 156 LEKVAEFFPTKYLKKDSFFTFSFFA-SGTAQITITTDEKEETTTAVENKNVVEMIKKWYL 215
           LE V EFF +KY KKDS  TF F A SGTA+I  +T+ K E+   VEN NVVEMIKKWYL
Sbjct: 127 LELVVEFFQSKYFKKDSVITFHFPASSGTAEIAFSTEGKGESKICVENANVVEMIKKWYL 186

Query: 216 GGSRSVTPSTIECLCNNLSAELS 238
           GG+R V+PSTI CL   LSAELS
Sbjct: 187 GGTRGVSPSTISCLATTLSAELS 209
[Close]
BLAST of Spo26569.1 vs. ExPASy Swiss-Prot
Match: CFI3_ARATH (Probable chalcone--flavonone isomerase 3 OS=Arabidopsis thaliana GN=CHI3 PE=1 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 1.900e-55
Identity = 116/204 (56.86%), Postives = 147/204 (72.06%), Query Frame = 1 

		  

Query: 36  VIVDDIAFAPQVTLTKSLPLLGYGCTELEIHFLQIKFTAIGVYME-REVVQHLQKWKGKS 95
           V+V ++ F PQ+  +K L LLG G T++EIHFLQ+KFTAIGVY++  +V  HL  WKGK+
Sbjct: 6   VMVHEVPFPPQIITSKPLSLLGQGITDIEIHFLQVKFTAIGVYLDPSDVKTHLDNWKGKT 65

Query: 96  GKELADDDDFFDALTAAPVETVIKAVVIKEIKASQLGTQLEGSVRDRLAAADKYEEEEEA 155
           GKELA DDDFFDAL +A +E VI+ VVIKEIK +Q G QLE +VRDRLA  DKYEEEEE 
Sbjct: 66  GKELAGDDDFFDALASAEMEKVIRVVVIKEIKGAQYGVQLENTVRDRLAEEDKYEEEEET 125

Query: 156 ALEKVAEFFPTKYLKKDSFFTFSFFA-SGTAQITITTDEKEETTTAVENKNVVEMIKKWY 215
            LEKV  FF +KY K +S  T+ F A  G  +I   T+ KEE    VEN NVV M+++WY
Sbjct: 126 ELEKVVGFFQSKYFKANSVITYHFSAKDGICEIGFETEGKEEEKLKVENANVVGMMQRWY 185

Query: 216 LGGSRSVTPSTIECLCNNLSAELS 238
           L GSR V+PSTI  + +++SA L+
Sbjct: 186 LSGSRGVSPSTIVSIADSISAVLT 209
[Close]
BLAST of Spo26569.1 vs. ExPASy Swiss-Prot
Match: CFI_VERHY (Chalcone--flavonone isomerase OS=Verbena hybrida GN=CHI PE=2 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 8.000e-14
Identity = 62/205 (30.24%), Postives = 96/205 (46.83%), Query Frame = 1 

		  

Query: 35  DVIVDDIAFAPQVT---LTKSLPLLGYGCTELEIHFLQIKFTAIGVYMEREVVQHLQ-KW 94
           +V V+ + F P V      K+  L G G   LEI    IKFTAIGVY+E + V  L  KW
Sbjct: 9   EVQVESVVFPPAVKPPGSDKTFFLGGAGVRGLEIEGKFIKFTAIGVYLENDAVTSLAAKW 68

Query: 95  KGKSGKELADDDDFFDALTAAPVETVIKAVVIKEIKASQLGTQLEGSVRDRLAAADKYEE 154
           KGK+ +E+AD  DFF  +   P E   K   I  +   Q   ++  +      A  K+ +
Sbjct: 69  KGKTAEEVADSGDFFAEIVTGPFEKFTKVTTILPLTGQQYSEKVVENCVAYWKAIGKFTD 128

Query: 155 EEEAALEKVAEFFPTKYLKKDSFFTFSFFASGTAQITITTDEK--EETTTAVENKNVVEM 214
            E+ A +K  + F  +     +   F+    G+  I+ + D    E+    +ENK + E 
Sbjct: 129 AEDEATKKFLQVFKNEMFHPGASILFTQSPQGSLTISFSKDGSIPEKENAIIENKQLSEA 188

Query: 215 IKKWYLGGSRSVTPSTIECLCNNLS 234
           + +  + G + V+PS  + L   LS
Sbjct: 189 VLESII-GKKGVSPSAKQSLAARLS 212
[Close]
BLAST of Spo26569.1 vs. ExPASy Swiss-Prot
Match: CFI1_LOTJA (Chalcone--flavonone isomerase 1 OS=Lotus japonicus GN=CHI1 PE=2 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.200e-12
Identity = 62/201 (30.85%), Postives = 94/201 (46.77%), Query Frame = 1 

		  

Query: 38  VDDIAFAPQVT---LTKSLPLLGYGCTELEIHFLQIKFTAIGVYMEREVVQHLQ-KWKGK 97
           V+++ F   VT     KS  L G G   L I    IKFT IGVY+E   V  L  KWKGK
Sbjct: 14  VENLQFPASVTSPATAKSYFLGGAGERGLTIEGKFIKFTGIGVYLEDTAVDSLATKWKGK 73

Query: 98  SGKELADDDDFFDALTAAPVETVIKAVVIKEIKASQLGTQLEGSVRDRLAAADKYEEEEE 157
           S +EL D  DFF  + ++P E +I+   ++ +   +   ++  +    + +A  Y E E 
Sbjct: 74  SSQELQDSLDFFRDIISSPSEKLIRGSKLRPLSGVEYSRKVMENCVAHMKSAGTYGEAEA 133

Query: 158 AALEKVAEFFPTKYLKKDSFFTFSFFASGTAQITITTDE--KEETTTAVENKNVVEMIKK 217
            A+EK AE F        S   +     G   ++ + D+   EE    +ENK + E + +
Sbjct: 134 TAIEKFAEAFRKVDFPPGSSVFYRQSTDGKLGLSFSLDDTIPEEEAVVIENKALSEAVLE 193

Query: 218 WYLGGSRSVTPSTIECLCNNL 233
             + G  +V+P    CL   L
Sbjct: 194 TMI-GEHAVSPDLKRCLAERL 213
[Close]
BLAST of Spo26569.1 vs. ExPASy Swiss-Prot
Match: CFI_EUSER (Chalcone--flavonone isomerase OS=Eustoma exaltatum subsp. russellianum GN=CHI PE=2 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 2.000e-12
Identity = 61/210 (29.05%), Postives = 98/210 (46.67%), Query Frame = 1 

		  

Query: 34  NDVIVDDIAFAPQVT---LTKSLPLLGYGCTELEIHFLQIKFTAIGVYMEREVVQHLQ-K 93
           ++V V+   F P +T    TKS  L G G   LEI    +KFTAIG+Y++   +  L  K
Sbjct: 8   SEVQVESYLFPPTITPPASTKSFLLGGAGVRGLEIGGNFVKFTAIGIYLDETAIPWLAGK 67

Query: 94  WKGKSGKELADDDDFFDALTAAPVETVIKAVVIKEIKASQLGTQLEGSVRDRLAAADKYE 153
           WKGK+ +ELAD  DFF  +   P E   +  +I  +   Q   ++  +   +  AA  Y 
Sbjct: 68  WKGKTAEELADSVDFFADIITGPFEKFTQVTLILPLTGQQYSGKVSENCTAQWKAAGVYT 127

Query: 154 EEEEAALEKVAEFF-PTKYLKKDSFFTFSFFASGTAQITITTDEKEETTTAVENKNVVEM 213
             +  A+EK  + F P  +   DS        S T       D  E +   +ENK + E 
Sbjct: 128 PADGKAIEKFLQVFEPQSFSPGDSILFMHSPESLTISFGKAGDIPETSNAVIENKKLSEA 187

Query: 214 IKKWYLGGSRSVTPSTIECLCNNLSAELSN 239
           + +  + G + V+P+  + L   ++  L++
Sbjct: 188 VIESVI-GEKGVSPAAKKSLATRIAHVLNH 216
[Close]
BLAST of Spo26569.1 vs. ExPASy Swiss-Prot
Match: CFI_GENTR (Chalcone--flavonone isomerase OS=Gentiana triflora GN=CHI PE=2 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 5.800e-12
Identity = 64/213 (30.05%), Postives = 103/213 (48.36%), Query Frame = 1 

		  

Query: 35  DVIVDDIAFAPQV---TLTKSLPLLGYGCTELEIHFLQIKFTAIGVYMEREVVQHLQ-KW 94
           +V V+   F P V   + TKS  L G G   LEI+   +KFTAIGVY+E   V  L  KW
Sbjct: 11  EVKVESYVFPPSVKPPSSTKSFLLGGAGVRGLEINGNFVKFTAIGVYLEESGVAVLSGKW 70

Query: 95  KGKSGKELADDDDFFDALTAAPVETVIKAVVIKEIKASQLGTQLEGSVRDRLAAADKYEE 154
           KGK+ +EL+D  +FF  +   P E   +  +I  +   Q   ++  +   +  AA  Y +
Sbjct: 71  KGKTAEELSDSVEFFTDIITGPFEKFTQVTLILPVTGQQYSPKVAENCAAQWKAAGIYTD 130

Query: 155 EEEAALEKVAEFFPTK-YLKKDSFFTFSFFASGTAQITITTDEK----EETTTAVENKNV 214
            +  A+EK  + F T+ +   DS      F      +TI+  +     E +   +ENK +
Sbjct: 131 ADGIAIEKFLQVFQTESFTPGDSI----LFTHSPESLTISFGKNGAIPEVSNAVIENKKL 190

Query: 215 VEMIKKWYLGGSRSVTPSTIECLCNNLSAELSN 239
            E + +  + G + V+P+  + L   + AE+ N
Sbjct: 191 SEAVIESII-GEKGVSPAAKKSLATRI-AEILN 217
[Close]
BLAST of Spo26569.1 vs. TAIR (Arabidopsis)
Match: AT5G05270.1 (Chalcone-flavanone isomerase family protein)

HSP 1 Score: 217.2 bits (552), Expect = 1.100e-56
Identity = 116/204 (56.86%), Postives = 147/204 (72.06%), Query Frame = 1 

		  

Query: 36  VIVDDIAFAPQVTLTKSLPLLGYGCTELEIHFLQIKFTAIGVYME-REVVQHLQKWKGKS 95
           V+V ++ F PQ+  +K L LLG G T++EIHFLQ+KFTAIGVY++  +V  HL  WKGK+
Sbjct: 6   VMVHEVPFPPQIITSKPLSLLGQGITDIEIHFLQVKFTAIGVYLDPSDVKTHLDNWKGKT 65

Query: 96  GKELADDDDFFDALTAAPVETVIKAVVIKEIKASQLGTQLEGSVRDRLAAADKYEEEEEA 155
           GKELA DDDFFDAL +A +E VI+ VVIKEIK +Q G QLE +VRDRLA  DKYEEEEE 
Sbjct: 66  GKELAGDDDFFDALASAEMEKVIRVVVIKEIKGAQYGVQLENTVRDRLAEEDKYEEEEET 125

Query: 156 ALEKVAEFFPTKYLKKDSFFTFSFFA-SGTAQITITTDEKEETTTAVENKNVVEMIKKWY 215
            LEKV  FF +KY K +S  T+ F A  G  +I   T+ KEE    VEN NVV M+++WY
Sbjct: 126 ELEKVVGFFQSKYFKANSVITYHFSAKDGICEIGFETEGKEEEKLKVENANVVGMMQRWY 185

Query: 216 LGGSRSVTPSTIECLCNNLSAELS 238
           L GSR V+PSTI  + +++SA L+
Sbjct: 186 LSGSRGVSPSTIVSIADSISAVLT 209
[Close]
BLAST of Spo26569.1 vs. TAIR (Arabidopsis)
Match: AT3G55120.1 (Chalcone-flavanone isomerase family protein)

HSP 1 Score: 62.4 bits (150), Expect = 4.400e-10
Identity = 61/206 (29.61%), Postives = 87/206 (42.23%), Query Frame = 1 

		  

Query: 38  VDDIAFAPQVTLTKS---LPLLGYGCTELEIHFLQIKFTAIGVYMEREVVQHLQ-KWKGK 97
           VD + F P V    S   L L G G   L+I    + FT IGVY+E   V  L  KWKGK
Sbjct: 21  VDSVTFVPSVKSPASSNPLFLGGAGVRGLDIQGKFVIFTVIGVYLEGNAVPSLSVKWKGK 80

Query: 98  SGKELADDDDFFDALTAAPVETVIKAVVIKEIKASQLGTQLEGSVRDRLAAADK----YE 157
           + +EL +   FF  +     E  IK      +K    G Q    V +   A  K    Y 
Sbjct: 81  TTEELTESIPFFREIVTGAFEKFIKVT----MKLPLTGQQYSEKVTENCVAIWKQLGLYT 140

Query: 158 EEEEAALEKVAEFFPTKYLKKDSFFTFSFFASGTAQITITTDEK--EETTTAVENKNVVE 217
           + E  A+EK  E F  +     S   F+   +G+  +  + D+   E     +ENK + E
Sbjct: 141 DCEAKAVEKFLEIFKEETFPPGSSILFALSPTGSLTVAFSKDDSIPETGIAVIENKLLAE 200

Query: 218 MIKKWYLGGSRSVTPSTIECLCNNLS 234
            + +  + G   V+P T   +   LS
Sbjct: 201 AVLESII-GKNGVSPGTRLSVAERLS 221
The following BLAST results are available for this feature:
BLAST of Spo26569.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|731320056|ref|XP_010670602.1|1.6e-9486.8PREDICTED: probable chalcone--... [more]
gi|870865722|gb|KMT16760.1|7.3e-9287.0hypothetical protein BVRB_2g04... [more]
gi|816196829|dbj|BAR72208.1|5.6e-8479.9enhancer of flavonoid producti... [more]
gi|902226877|gb|KNA20513.1|2.2e-80100.hypothetical protein SOVF_0516... [more]
gi|444475579|gb|AGE10598.1|1.1e-6866.1chalcone isomerase [Lonicera j... [more]
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BLAST of Spo26569.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0J8CSJ0_BETVU5.1e-9287.0Chalcone-flavonone isomerase f... [more]
A0A0K9RLZ4_SPIOL1.5e-80100.Chalcone-flavonone isomerase f... [more]
L7Z972_LONJA8.0e-6966.1Chalcone-flavonone isomerase f... [more]
A0A0A1G7T9_9ERIC3.9e-6867.9Chalcone-flavonone isomerase f... [more]
T2DL26_ACTCH2.0e-6767.0Chalcone-flavonone isomerase f... [more]
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BLAST of Spo26569.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5
Match NameE-valueIdentityDescription
CFI3_ARATH1.9e-5556.8Probable chalcone--flavonone i... [more]
CFI_VERHY8.0e-1430.2Chalcone--flavonone isomerase ... [more]
CFI1_LOTJA1.2e-1230.8Chalcone--flavonone isomerase ... [more]
CFI_EUSER2.0e-1229.0Chalcone--flavonone isomerase ... [more]
CFI_GENTR5.8e-1230.0Chalcone--flavonone isomerase ... [more]
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BLAST of Spo26569.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 2
Match NameE-valueIdentityDescription
AT5G05270.11.1e-5656.8Chalcone-flavanone isomerase f... [more]
AT3G55120.14.4e-1029.6Chalcone-flavanone isomerase f... [more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016087Chalcone isomerasePFAMPF02431Chalconecoord: 38..234
score: 1.3
IPR016087Chalcone isomeraseunknownSSF54626Chalcone isomerasecoord: 36..237
score: 2.35
IPR016088Chalcone isomerase, 3-layer sandwichGENE3D3.50.70.10coord: 35..213
score: 4.1
NoneNo IPR availablePANTHERPTHR28039FAMILY NOT NAMEDcoord: 36..238
score: 2.4
NoneNo IPR availablePANTHERPTHR28039:SF4CHALCONE--FLAVONONE ISOMERASE 3-RELATEDcoord: 36..238
score: 2.4

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009813 flavonoid biosynthetic process
biological_process GO:0009805 coumarin biosynthetic process
biological_process GO:0006855 drug transmembrane transport
biological_process GO:0050896 response to stimulus
biological_process GO:0009809 lignin biosynthetic process
biological_process GO:0009811 stilbene biosynthetic process
biological_process GO:0008152 metabolic process
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0009706 chloroplast inner membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016872 intramolecular lyase activity
molecular_function GO:0016887 ATPase activity
molecular_function GO:0015238 drug transmembrane transporter activity
molecular_function GO:0015297 antiporter activity
molecular_function GO:0047077 Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing) activity
molecular_function GO:0045430 chalcone isomerase activity
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0016207 4-coumarate-CoA ligase activity
molecular_function GO:0016757 transferase activity, transferring glycosyl groups
molecular_function GO:0003723 RNA binding
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0045486 naringenin 3-dioxygenase activity
RNA-Seq Expression